JP2006521829A5 - - Google Patents
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- JP2006521829A5 JP2006521829A5 JP2006509721A JP2006509721A JP2006521829A5 JP 2006521829 A5 JP2006521829 A5 JP 2006521829A5 JP 2006509721 A JP2006509721 A JP 2006509721A JP 2006509721 A JP2006509721 A JP 2006509721A JP 2006521829 A5 JP2006521829 A5 JP 2006521829A5
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Families Citing this family (165)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6221654B1 (en) | 1996-09-25 | 2001-04-24 | California Institute Of Technology | Method and apparatus for analysis and sorting of polynucleotides based on size |
| US7214298B2 (en) * | 1997-09-23 | 2007-05-08 | California Institute Of Technology | Microfabricated cell sorter |
| US7144616B1 (en) | 1999-06-28 | 2006-12-05 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| US8052792B2 (en) | 2001-04-06 | 2011-11-08 | California Institute Of Technology | Microfluidic protein crystallography techniques |
| US8709153B2 (en) | 1999-06-28 | 2014-04-29 | California Institute Of Technology | Microfludic protein crystallography techniques |
| US7052545B2 (en) * | 2001-04-06 | 2006-05-30 | California Institute Of Technology | High throughput screening of crystallization of materials |
| US7459022B2 (en) | 2001-04-06 | 2008-12-02 | California Institute Of Technology | Microfluidic protein crystallography |
| US7306672B2 (en) | 2001-04-06 | 2007-12-11 | California Institute Of Technology | Microfluidic free interface diffusion techniques |
| IL147302A0 (en) | 1999-06-28 | 2002-08-14 | California Inst Of Techn | Microfabricated elastomeric valve and pump systems |
| US7867763B2 (en) * | 2004-01-25 | 2011-01-11 | Fluidigm Corporation | Integrated chip carriers with thermocycler interfaces and methods of using the same |
| US20050118073A1 (en) | 2003-11-26 | 2005-06-02 | Fluidigm Corporation | Devices and methods for holding microfluidic devices |
| US7351376B1 (en) | 2000-06-05 | 2008-04-01 | California Institute Of Technology | Integrated active flux microfluidic devices and methods |
| WO2002023163A1 (en) | 2000-09-15 | 2002-03-21 | California Institute Of Technology | Microfabricated crossflow devices and methods |
| US7378280B2 (en) * | 2000-11-16 | 2008-05-27 | California Institute Of Technology | Apparatus and methods for conducting assays and high throughput screening |
| US8440093B1 (en) | 2001-10-26 | 2013-05-14 | Fuidigm Corporation | Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels |
| US7118910B2 (en) * | 2001-11-30 | 2006-10-10 | Fluidigm Corporation | Microfluidic device and methods of using same |
| US7691333B2 (en) * | 2001-11-30 | 2010-04-06 | Fluidigm Corporation | Microfluidic device and methods of using same |
| CA2480728A1 (en) | 2002-04-01 | 2003-10-16 | Fluidigm Corporation | Microfluidic particle-analysis systems |
| US8220494B2 (en) * | 2002-09-25 | 2012-07-17 | California Institute Of Technology | Microfluidic large scale integration |
| JP2006501056A (ja) * | 2002-09-25 | 2006-01-12 | カリフォルニア インスティテュート オブ テクノロジー | ミクロ流体大規模集積 |
| AU2003299541A1 (en) | 2002-10-02 | 2004-05-25 | California Institute Of Technology | Microfluidic nucleic acid analysis |
| US7419638B2 (en) * | 2003-01-14 | 2008-09-02 | Micronics, Inc. | Microfluidic devices for fluid manipulation and analysis |
| US7476363B2 (en) * | 2003-04-03 | 2009-01-13 | Fluidigm Corporation | Microfluidic devices and methods of using same |
| US20050145496A1 (en) | 2003-04-03 | 2005-07-07 | Federico Goodsaid | Thermal reaction device and method for using the same |
| US7604965B2 (en) | 2003-04-03 | 2009-10-20 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| US8828663B2 (en) * | 2005-03-18 | 2014-09-09 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| WO2004103563A2 (en) | 2003-05-20 | 2004-12-02 | Fluidigm Corporation | Method and system for microfluidic device and imaging thereof |
| SG145697A1 (en) * | 2003-07-28 | 2008-09-29 | Fluidigm Corp | Image processing method and system for microfluidic devices |
| US7413712B2 (en) | 2003-08-11 | 2008-08-19 | California Institute Of Technology | Microfluidic rotary flow reactor matrix |
| US20050047967A1 (en) * | 2003-09-03 | 2005-03-03 | Industrial Technology Research Institute | Microfluidic component providing multi-directional fluid movement |
| DE102004062281A1 (de) * | 2003-12-29 | 2005-07-28 | Siemens Ag | Verfahren und Spotting-Lösung zum Herstellen von Microarrays |
| US7407799B2 (en) | 2004-01-16 | 2008-08-05 | California Institute Of Technology | Microfluidic chemostat |
| SG10201405756WA (en) * | 2004-01-25 | 2014-11-27 | Fluidigm Corp | Crystal forming devices and systems and methods for making and using the same |
| US8058056B2 (en) * | 2004-03-12 | 2011-11-15 | The Regents Of The University Of California | Method and apparatus for integrated cell handling and measurements |
| WO2005107938A2 (en) * | 2004-05-02 | 2005-11-17 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| US20060030036A1 (en) * | 2004-05-28 | 2006-02-09 | Victor Joseph | Chips for multiplex analyses |
| EP2752874A1 (en) | 2004-06-07 | 2014-07-09 | Fluidigm Corporation | Method and apparatus for imaging a device under temperature control |
| EP1838431A4 (en) * | 2004-12-03 | 2012-08-22 | California Inst Of Techn | MICROFLUIDIC DEVICES WITH CIRCUITRY OF CHEMICAL REACTIONS |
| WO2006060748A2 (en) | 2004-12-03 | 2006-06-08 | California Institute Of Technology | Microfluidic sieve valves |
| EP1861512A4 (en) * | 2005-03-18 | 2009-12-09 | Fluidigm Corp | THERMAL REACTION DEVICE AND USE METHOD THEREFOR |
| EP1882189A2 (en) | 2005-04-20 | 2008-01-30 | Fluidigm Corporation | Analysis engine and database for manipulating parameters for fluidic systems on a chip |
| EP2477029A1 (en) * | 2005-06-02 | 2012-07-18 | Fluidigm Corporation | Analysis using microfluidic partitioning devices |
| US20070054293A1 (en) * | 2005-08-30 | 2007-03-08 | California Institute Of Technology | Microfluidic chaotic mixing systems and methods |
| US7598788B2 (en) * | 2005-09-06 | 2009-10-06 | Broadcom Corporation | Current-controlled CMOS (C3MOS) fully differential integrated delay cell with variable delay and high bandwidth |
| WO2007033385A2 (en) * | 2005-09-13 | 2007-03-22 | Fluidigm Corporation | Microfluidic assay devices and methods |
| US20070107809A1 (en) * | 2005-11-14 | 2007-05-17 | The Regents Of The Univerisity Of California | Process for making corrosion-resistant amorphous-metal coatings from gas-atomized amorphous-metal powders having relatively high critical cooling rates through particle-size optimization (PSO) and variations thereof |
| US8480864B2 (en) * | 2005-11-14 | 2013-07-09 | Joseph C. Farmer | Compositions of corrosion-resistant Fe-based amorphous metals suitable for producing thermal spray coatings |
| US7618500B2 (en) | 2005-11-14 | 2009-11-17 | Lawrence Livermore National Security, Llc | Corrosion resistant amorphous metals and methods of forming corrosion resistant amorphous metals |
| TR201910868T4 (tr) * | 2006-02-02 | 2019-08-21 | Univ Leland Stanford Junior | Dijital analizle invazif olmayan fetal genetik tarama. |
| US11237171B2 (en) | 2006-02-21 | 2022-02-01 | Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of target analyte concentration in solution |
| US8492098B2 (en) | 2006-02-21 | 2013-07-23 | The Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of reaction components that affect a reaction |
| US7815868B1 (en) * | 2006-02-28 | 2010-10-19 | Fluidigm Corporation | Microfluidic reaction apparatus for high throughput screening |
| CN100392316C (zh) * | 2006-03-27 | 2008-06-04 | 博奥生物有限公司 | 控制液体在微管路中连续流动的流路结构 |
| US8293524B2 (en) * | 2006-03-31 | 2012-10-23 | Fluxion Biosciences Inc. | Methods and apparatus for the manipulation of particle suspensions and testing thereof |
| US20090302228A1 (en) * | 2006-04-20 | 2009-12-10 | The Regents Of The University Of California | Device for quantification of radioisotope concentrations in a micro-fluidic platform |
| US8828661B2 (en) * | 2006-04-24 | 2014-09-09 | Fluidigm Corporation | Methods for detection and quantification of nucleic acid or protein targets in a sample |
| US8245661B2 (en) * | 2006-06-05 | 2012-08-21 | Lawrence Livermore National Security, Llc | Magnetic separation of devitrified particles from corrosion-resistant iron-based amorphous metal powders |
| WO2007147074A2 (en) | 2006-06-14 | 2007-12-21 | Living Microsystems, Inc. | Use of highly parallel snp genotyping for fetal diagnosis |
| US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
| EP2589668A1 (en) * | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Rare cell analysis using sample splitting and DNA tags |
| US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
| DE102006030068A1 (de) * | 2006-06-28 | 2008-01-03 | M2P-Labs Gmbh | Vorrichtung und Verfahren zur Zu- und Abfuhr von Fluiden in geschüttelten Mikroreaktoren Arrays |
| US8055034B2 (en) | 2006-09-13 | 2011-11-08 | Fluidigm Corporation | Methods and systems for image processing of microfluidic devices |
| WO2008067552A2 (en) * | 2006-11-30 | 2008-06-05 | Fluidigm Corporation | Method and apparatus for biological sample analysis |
| US8681743B2 (en) * | 2006-12-08 | 2014-03-25 | Samsung Electronics Co., Ltd. | Apparatus and method for selecting frame structure in multihop relay broadband wireless access communication system |
| GB2445738A (en) * | 2007-01-16 | 2008-07-23 | Lab901 Ltd | Microfluidic device |
| EP2125219B1 (en) | 2007-01-19 | 2016-08-10 | Fluidigm Corporation | High precision microfluidic devices and methods |
| WO2008091626A1 (en) | 2007-01-22 | 2008-07-31 | Wafergen, Inc. | Apparatus for high throughput chemical reactions |
| LT2557517T (lt) | 2007-07-23 | 2023-01-10 | The Chinese University Of Hong Kong | Nukleino rūgščių sekos disbalanso nustatymas |
| US12180549B2 (en) | 2007-07-23 | 2024-12-31 | The Chinese University Of Hong Kong | Diagnosing fetal chromosomal aneuploidy using genomic sequencing |
| JP2009042103A (ja) * | 2007-08-09 | 2009-02-26 | Sony Corp | 基板、これを用いた反応処理装置並びに反応制御方法 |
| WO2009029073A1 (en) | 2007-08-30 | 2009-03-05 | The Trustees Of Tufts College | Methods for determining the concentration of an analyte in solution. |
| CA2698545C (en) | 2007-09-07 | 2014-07-08 | Fluidigm Corporation | Copy number variation determination, methods and systems |
| EP2212437A4 (en) | 2007-11-07 | 2011-09-28 | Univ British Columbia | MICROFLUIDIC DEVICE AND METHOD OF USING THE DEVICE |
| US9157116B2 (en) | 2008-02-08 | 2015-10-13 | Fluidigm Corporation | Combinatorial amplification and detection of nucleic acids |
| CN102056838B (zh) | 2008-04-11 | 2013-07-03 | 弗卢丁公司 | 微流体装置和方法 |
| JPWO2009131043A1 (ja) * | 2008-04-25 | 2011-08-18 | コニカミノルタエムジー株式会社 | マイクロチップ |
| JP5109824B2 (ja) * | 2008-06-16 | 2012-12-26 | 凸版印刷株式会社 | 反応チップ処理装置 |
| SG2014012777A (en) | 2008-07-25 | 2014-06-27 | Fluidigm Corp | Method and system for manufacturing integratedfluidic chips |
| US8617488B2 (en) | 2008-08-07 | 2013-12-31 | Fluidigm Corporation | Microfluidic mixing and reaction systems for high efficiency screening |
| HUE031848T2 (en) | 2008-09-20 | 2017-08-28 | Univ Leland Stanford Junior | Non-invasive diagnosis of fetal aneuploidy by sequencing |
| CA2738578C (en) * | 2008-09-23 | 2021-05-04 | Quantalife, Inc. | Droplet-based assay system |
| EP2349566B1 (en) | 2008-10-03 | 2016-01-06 | Micronics, Inc. | Microfluidic apparatus and methods for performing blood typing and crossmatching |
| WO2011109440A1 (en) | 2010-03-01 | 2011-09-09 | Caris Life Sciences Luxembourg Holdings | Biomarkers for theranostics |
| US8058630B2 (en) | 2009-01-16 | 2011-11-15 | Fluidigm Corporation | Microfluidic devices and methods |
| US8790916B2 (en) | 2009-05-14 | 2014-07-29 | Genestream, Inc. | Microfluidic method and system for isolating particles from biological fluid |
| US8551787B2 (en) * | 2009-07-23 | 2013-10-08 | Fluidigm Corporation | Microfluidic devices and methods for binary mixing |
| SG169918A1 (en) | 2009-10-02 | 2011-04-29 | Fluidigm Corp | Microfluidic devices with removable cover and methods of fabrication and application |
| CN102686246B (zh) | 2009-11-30 | 2016-04-06 | 富鲁达公司 | 微流体装置的再生 |
| US8236574B2 (en) | 2010-03-01 | 2012-08-07 | Quanterix Corporation | Ultra-sensitive detection of molecules or particles using beads or other capture objects |
| WO2011127219A1 (en) | 2010-04-06 | 2011-10-13 | Caris Life Sciences Luxembourg Holdings | Circulating biomarkers for disease |
| EP2567395B1 (en) * | 2010-05-07 | 2019-12-18 | UT-Battelle, LLC | System and method for extracting a sample from a surface |
| EP2582850B1 (en) * | 2010-06-21 | 2016-11-09 | Life Technologies Corporation | Compositions, kits, and methods for synthesis and/or detection of nucleic acids |
| US8486703B2 (en) | 2010-09-30 | 2013-07-16 | Ut-Battelle, Llc | Surface sampling concentration and reaction probe |
| US8519330B2 (en) | 2010-10-01 | 2013-08-27 | Ut-Battelle, Llc | Systems and methods for laser assisted sample transfer to solution for chemical analysis |
| US9353406B2 (en) | 2010-10-22 | 2016-05-31 | Fluidigm Corporation | Universal probe assay methods |
| US9952237B2 (en) | 2011-01-28 | 2018-04-24 | Quanterix Corporation | Systems, devices, and methods for ultra-sensitive detection of molecules or particles |
| US9168531B2 (en) | 2011-03-24 | 2015-10-27 | Fluidigm Corporation | Method for thermal cycling of microfluidic samples |
| WO2012142301A2 (en) | 2011-04-12 | 2012-10-18 | Quanterix Corporation | Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury |
| US9644231B2 (en) | 2011-05-09 | 2017-05-09 | Fluidigm Corporation | Nucleic acid detection using probes |
| SG194722A1 (en) | 2011-05-09 | 2013-12-30 | Fluidigm Corp | Probe based nucleic acid detection |
| JP4934750B1 (ja) * | 2011-05-31 | 2012-05-16 | 株式会社メトラン | ポンプユニット、呼吸補助装置 |
| WO2013126483A1 (en) | 2012-02-21 | 2013-08-29 | Fluidigm Corporation | Method and systems for microfluidic logic devices |
| JP5636555B2 (ja) * | 2012-04-02 | 2014-12-10 | 株式会社メトラン | ポンプユニット、呼吸補助装置 |
| EP2852682B1 (en) | 2012-05-21 | 2017-10-04 | Fluidigm Corporation | Single-particle analysis of particle populations |
| AU2013340414B2 (en) | 2012-10-23 | 2019-05-02 | Caris Science, Inc. | Aptamers and uses thereof |
| US10942184B2 (en) | 2012-10-23 | 2021-03-09 | Caris Science, Inc. | Aptamers and uses thereof |
| US9857333B2 (en) | 2012-10-31 | 2018-01-02 | Berkeley Lights, Inc. | Pens for biological micro-objects |
| EP2935628B1 (en) | 2012-12-19 | 2018-03-21 | Caris Life Sciences Switzerland Holdings GmbH | Compositions and methods for aptamer screening |
| US9932626B2 (en) | 2013-01-15 | 2018-04-03 | Quanterix Corporation | Detection of DNA or RNA using single molecule arrays and other techniques |
| US9163277B2 (en) | 2013-03-14 | 2015-10-20 | Formulatrix, Inc. | Microfluidic device |
| US9039993B2 (en) | 2013-03-14 | 2015-05-26 | Formulatrix, Inc. | Microfluidic device |
| US20140323819A1 (en) * | 2013-04-29 | 2014-10-30 | Elwha LLC, a limited liability company of the State of Delaware | Multi-parameter test units for initial indication of medical symptoms |
| EP2994750B1 (en) | 2013-05-07 | 2020-08-12 | PerkinElmer Health Sciences, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
| WO2014207258A1 (en) * | 2013-06-28 | 2014-12-31 | Danmarks Tekniske Universitet | A microfluidic device with a diffusion barrier |
| US9834747B2 (en) | 2013-07-31 | 2017-12-05 | Massachusetts Institute Of Technology | Methods and apparatus for transplantation of nucleic acid molecules |
| AU2014312211A1 (en) | 2013-08-28 | 2016-03-10 | Caris Science, Inc. | Oligonucleotide probes and uses thereof |
| US9889445B2 (en) | 2013-10-22 | 2018-02-13 | Berkeley Lights, Inc. | Micro-fluidic devices for assaying biological activity |
| EP3060645B1 (en) | 2013-10-22 | 2019-01-02 | Berkeley Lights, Inc. | Microfluidic devices having isolation pens and methods of testing biological micro-objects with same |
| US11192107B2 (en) | 2014-04-25 | 2021-12-07 | Berkeley Lights, Inc. | DEP force control and electrowetting control in different sections of the same microfluidic apparatus |
| US20150352547A1 (en) | 2014-06-06 | 2015-12-10 | Berkeley Lights, Inc. | Isolating Microfluidic Structures and Trapping Bubbles |
| DE102014221309B4 (de) | 2014-10-21 | 2025-04-24 | Robert Bosch Gmbh | Mikrofluidisches System sowie Verfahren zum Analysieren einer Probenlösung und Verfahren zum Herstellen eines mikrofluidischen Systems zum Analysieren einer Probenlösung |
| KR102260910B1 (ko) * | 2014-11-11 | 2021-06-04 | 에이아이엠 바이오테크 피티이. 엘티디. | 세포 기반 상호 작용 연구를 위한 마이크로 유체 플랫폼 |
| WO2016094333A1 (en) * | 2014-12-08 | 2016-06-16 | Berkeley Lights, Inc. | Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof |
| WO2016094522A1 (en) | 2014-12-09 | 2016-06-16 | Berkeley Lights, Inc. | Automated detection of assay-positive areas in microfluidic devices |
| US9717644B2 (en) | 2014-12-22 | 2017-08-01 | John H. Shadduck | Wearable sensing and actuator systems, and methods of use |
| US10364467B2 (en) | 2015-01-13 | 2019-07-30 | The Chinese University Of Hong Kong | Using size and number aberrations in plasma DNA for detecting cancer |
| JP6620160B2 (ja) | 2015-02-20 | 2019-12-11 | タカラ バイオ ユーエスエー， インコーポレイテッド | 単一細胞の迅速かつ正確な分注、視覚化及び解析のための方法 |
| WO2016145128A1 (en) | 2015-03-09 | 2016-09-15 | Caris Science, Inc. | Oligonucleotide probes and uses thereof |
| US10167502B2 (en) | 2015-04-03 | 2019-01-01 | Fluxion Biosciences, Inc. | Molecular characterization of single cells and cell populations for non-invasive diagnostics |
| US10751715B1 (en) | 2015-04-22 | 2020-08-25 | Berkeley Lights, Inc. | Microfluidic reporter cell assay methods and kits thereof |
| AU2016252995B2 (en) | 2015-04-22 | 2020-12-03 | Berkeley Lights, Inc. | Freezing and archiving cells on a microfluidic device |
| US10723988B2 (en) | 2015-04-22 | 2020-07-28 | Berkeley Lights, Inc. | Microfluidic cell culture |
| CA2991045A1 (en) | 2015-06-29 | 2017-01-05 | Caris Science, Inc. | Therapeutic oligonucleotides binding c1q |
| AU2016298317B2 (en) | 2015-07-28 | 2021-02-18 | Caris Science, Inc. | Targeted oligonucleotides |
| US10799865B2 (en) | 2015-10-27 | 2020-10-13 | Berkeley Lights, Inc. | Microfluidic apparatus having an optimized electrowetting surface and related systems and methods |
| EP3380238A1 (en) | 2015-11-23 | 2018-10-03 | Berkeley Lights, Inc. | In situ-generated microfluidic isolation structures, kits and methods of use thereof |
| CA3005077C (en) | 2015-12-08 | 2023-02-28 | Berkeley Lights, Inc. | In situ-generated microfluidic assay structures, related kits, and methods of use thereof |
| KR102512607B1 (ko) | 2015-12-30 | 2023-03-21 | 버클리 라잇츠, 인크. | 광학적으로 구동되는 대류 및 변위를 위한 미세유체 디바이스들, 키트들 및 그 방법들 |
| WO2017117521A1 (en) | 2015-12-31 | 2017-07-06 | Berkeley Lights, Inc. | Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide |
| CA3011087A1 (en) | 2016-01-15 | 2017-07-20 | Berkeley Lights, Inc. | Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor |
| CN109922885B (zh) | 2016-03-16 | 2022-05-10 | 伯克利之光生命科技公司 | 用于基因组编辑克隆的选择和传代的方法、系统和装置 |
| EP4339288A3 (en) | 2016-03-18 | 2024-06-05 | Caris Science, Inc. | Oligonucleotide probes and uses thereof |
| US10675625B2 (en) | 2016-04-15 | 2020-06-09 | Berkeley Lights, Inc | Light sequencing and patterns for dielectrophoretic transport |
| EP4684881A3 (en) | 2016-04-15 | 2026-03-18 | Bruker Cellular Analysis, Inc. | Methods, systems, computer program and non-transitory computer-readable medium for in-pen assays |
| CA3025486A1 (en) | 2016-05-25 | 2017-11-30 | Caris Science, Inc. | Oligonucleotide probes and uses thereof |
| IL263274B2 (en) | 2016-05-26 | 2023-10-01 | Berkeley Lights Inc | Covalently adapted surfaces, kits and methods for their production and uses |
| WO2018018017A1 (en) | 2016-07-21 | 2018-01-25 | Berkeley Lights, Inc. | Sorting of t lymphocytes in a microfluidic device |
| CA3020629A1 (en) | 2016-07-21 | 2018-01-25 | Takara Bio Usa, Inc. | Multi-z imaging and dispensing with multi-well devices |
| CA3048645A1 (en) | 2016-12-30 | 2018-07-05 | The Regents Of The University Of California | Methods for selection and generation of genome edited t cells |
| WO2019075476A2 (en) | 2017-10-15 | 2019-04-18 | Berkeley Lights, Inc. | METHODS, SYSTEMS AND KITS FOR ENCLOSED TESTS |
| US11154864B2 (en) | 2018-01-17 | 2021-10-26 | Qiagen Sciences, Llc | Microfluidic device with vented microchambers |
| US11731126B2 (en) * | 2018-04-19 | 2023-08-22 | Nanyang Technological University | Microfluidic board and method of forming the same |
| CA3112813A1 (en) | 2018-09-21 | 2020-03-26 | Berkeley Lights, Inc. | Functionalized well plate, methods of preparation and use thereof |
| CN113195085B (zh) | 2018-10-11 | 2024-04-19 | 伯克利之光生命科技公司 | 用于识别优化蛋白产生的系统和方法以及其试剂盒 |
| CN113366312A (zh) | 2018-11-01 | 2021-09-07 | 伯克利之光生命科技公司 | 在微流体环境中测定生物细胞的方法 |
| JP7462632B2 (ja) | 2018-11-30 | 2024-04-05 | カリス エムピーアイ インコーポレイテッド | 次世代分子プロファイリング |
| WO2020168258A1 (en) | 2019-02-15 | 2020-08-20 | Berkeley Lights, Inc. | Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment |
| WO2020223555A1 (en) | 2019-04-30 | 2020-11-05 | Berkeley Lights, Inc. | Methods for encapsulating and assaying cells |
| WO2021112918A1 (en) | 2019-12-02 | 2021-06-10 | Caris Mpi, Inc. | Pan-cancer platinum response predictor |
| US11872551B2 (en) * | 2020-02-28 | 2024-01-16 | Revvity Health Sciences, Inc. | Multiplexed polymerase chain reaction in micropipette format |
| JP7561364B2 (ja) * | 2020-04-30 | 2024-10-04 | 国立大学法人東北大学 | 蛍光偏光免疫分析法 |
| AU2021338364A1 (en) | 2020-09-07 | 2023-03-30 | Berkeley Lights, Inc. | Methods of assaying a biological cell |
| US20240050950A1 (en) * | 2020-12-23 | 2024-02-15 | Cornell University | Gradient-based microfluidic circuit, device, and method for performing an assay |
| JPWO2024252453A1 (https=) * | 2023-06-05 | 2024-12-12 |
Family Cites Families (241)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3570515A (en) | 1969-06-19 | 1971-03-16 | Foxboro Co | Aminar stream cross-flow fluid diffusion logic gate |
| NL7102074A (https=) | 1971-02-17 | 1972-08-21 | ||
| US3839176A (en) | 1971-03-08 | 1974-10-01 | North American Rockwell | Method and apparatus for removing contaminants from liquids |
| US3984307A (en) | 1973-03-05 | 1976-10-05 | Bio/Physics Systems, Inc. | Combined particle sorter and segregation indicator |
| FR2287606A1 (fr) | 1974-10-08 | 1976-05-07 | Pegourie Jean Pierre | Circuits logiques pneumatiques et leurs circuits integres |
| JPS5941169B2 (ja) | 1975-12-25 | 1984-10-05 | シチズン時計株式会社 | エラストマ−ヒヨウジソウチ |
| US4153855A (en) | 1977-12-16 | 1979-05-08 | The United States Of America As Represented By The Secretary Of The Army | Method of making a plate having a pattern of microchannels |
| US4245673A (en) | 1978-03-01 | 1981-01-20 | La Telemechanique Electrique | Pneumatic logic circuit |
| US4373527B1 (en) | 1979-04-27 | 1995-06-27 | Univ Johns Hopkins | Implantable programmable medication infusion system |
| US4434704A (en) | 1980-04-14 | 1984-03-06 | Halliburton Company | Hydraulic digital stepper actuator |
| GB2097692B (en) | 1981-01-10 | 1985-05-22 | Shaw Stewart P D | Combining chemical reagents |
| FR2504010B1 (fr) | 1981-04-15 | 1985-10-25 | Sanofi Sa | Medicaments anticancereux contenant la chaine a de la ricine associee a un anticorps antimelanome et procede pour leur preparation |
| US4671958A (en) | 1982-03-09 | 1987-06-09 | Cytogen Corporation | Antibody conjugates for the delivery of compounds to target sites |
| DE3377484D1 (en) | 1982-04-19 | 1988-09-01 | Nissan Motor | Method for controlling reduction ratio of continuously variable transmission with acceleration compensation |
| US4575681A (en) | 1982-11-12 | 1986-03-11 | Teleco Oilfield Services Inc. | Insulating and electrode structure for a drill string |
| US4662710A (en) | 1982-12-03 | 1987-05-05 | Amp Incorporated | Method and apparatus for breaking an optical fiber |
| US4585209A (en) | 1983-10-27 | 1986-04-29 | Harry E. Aine | Miniature valve and method of making same |
| US4581624A (en) | 1984-03-01 | 1986-04-08 | Allied Corporation | Microminiature semiconductor valve |
| GB8408529D0 (en) | 1984-04-03 | 1984-05-16 | Health Lab Service Board | Concentration of biological particles |
| US4542225A (en) | 1984-08-29 | 1985-09-17 | Dana-Farber Cancer Institute, Inc. | Acid-cleavable compound |
| US4659839A (en) | 1984-10-10 | 1987-04-21 | Mallinckrodt, Inc. | Coupling agents for radiolabeled antibody fragments |
| JPH07104855B2 (ja) | 1985-03-28 | 1995-11-13 | インターナショナル・ビジネス・マシーンズ・コーポレーション | 数値シミュレーション装置 |
| US4963498A (en) | 1985-08-05 | 1990-10-16 | Biotrack | Capillary flow device |
| US4675300A (en) | 1985-09-18 | 1987-06-23 | The Board Of Trustees Of The Leland Stanford Junior University | Laser-excitation fluorescence detection electrokinetic separation |
| US4680338A (en) | 1985-10-17 | 1987-07-14 | Immunomedics, Inc. | Bifunctional linker |
| US4669784A (en) | 1986-02-24 | 1987-06-02 | Palmer Grasse | Oscillated head and connecting element, with bearing support, for floor stripping machine |
| US5088515A (en) | 1989-05-01 | 1992-02-18 | Kamen Dean L | Valve system with removable fluid interface |
| US4786165A (en) | 1986-07-10 | 1988-11-22 | Toa Medical Electronics Co., Ltd. | Flow cytometry and apparatus therefor |
| US5525464A (en) | 1987-04-01 | 1996-06-11 | Hyseq, Inc. | Method of sequencing by hybridization of oligonucleotide probes |
| WO1989001049A1 (en) | 1987-07-31 | 1989-02-09 | Gen-Probe Incorporated | Assay for polynucleotides employing oligonucleotides to eliminate undesirable cross reactions |
| US4990216A (en) | 1987-10-27 | 1991-02-05 | Fujitsu Limited | Process and apparatus for preparation of single crystal of biopolymer |
| US4843155A (en) | 1987-11-19 | 1989-06-27 | Piotr Chomczynski | Product and process for isolating RNA |
| US4936465A (en) | 1987-12-07 | 1990-06-26 | Zoeld Tibor | Method and apparatus for fast, reliable, and environmentally safe dispensing of fluids, gases and individual particles of a suspension through pressure control at well defined parts of a closed flow-through system |
| US5354695A (en) | 1992-04-08 | 1994-10-11 | Leedy Glenn J | Membrane dielectric isolation IC fabrication |
| US4908112A (en) | 1988-06-16 | 1990-03-13 | E. I. Du Pont De Nemours & Co. | Silicon semiconductor wafer for analyzing micronic biological samples |
| US5132012A (en) | 1988-06-24 | 1992-07-21 | Hitachi, Ltd. | Liquid chromatograph |
| US4965743A (en) | 1988-07-14 | 1990-10-23 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Discrete event simulation tool for analysis of qualitative models of continuous processing system |
| US4898582A (en) | 1988-08-09 | 1990-02-06 | Pharmetrix Corporation | Portable infusion device assembly |
| US5032381A (en) | 1988-12-20 | 1991-07-16 | Tropix, Inc. | Chemiluminescence-based static and flow cytometry |
| US5856092A (en) | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
| US4992312A (en) | 1989-03-13 | 1991-02-12 | Dow Corning Wright Corporation | Methods of forming permeation-resistant, silicone elastomer-containing composite laminates and devices produced thereby |
| CH679555A5 (https=) | 1989-04-11 | 1992-03-13 | Westonbridge Int Ltd | |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US6040138A (en) | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
| US5224843A (en) | 1989-06-14 | 1993-07-06 | Westonbridge International Ltd. | Two valve micropump with improved outlet |
| US5100627A (en) | 1989-11-30 | 1992-03-31 | The Regents Of The University Of California | Chamber for the optical manipulation of microscopic particles |
| KR910012538A (ko) | 1989-12-27 | 1991-08-08 | 야마무라 가쯔미 | 마이크로 펌프 및 그 제조 방법 |
| DE4006152A1 (de) | 1990-02-27 | 1991-08-29 | Fraunhofer Ges Forschung | Mikrominiaturisierte pumpe |
| US5126022A (en) | 1990-02-28 | 1992-06-30 | Soane Tecnologies, Inc. | Method and device for moving molecules by the application of a plurality of electrical fields |
| US5750015A (en) | 1990-02-28 | 1998-05-12 | Soane Biosciences | Method and device for moving molecules by the application of a plurality of electrical fields |
| US5096388A (en) | 1990-03-22 | 1992-03-17 | The Charles Stark Draper Laboratory, Inc. | Microfabricated pump |
| SE470347B (sv) | 1990-05-10 | 1994-01-31 | Pharmacia Lkb Biotech | Mikrostruktur för vätskeflödessystem och förfarande för tillverkning av ett sådant system |
| EP0465229B1 (en) | 1990-07-02 | 1994-12-28 | Seiko Epson Corporation | Micropump and process for manufacturing a micropump |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5846710A (en) | 1990-11-02 | 1998-12-08 | St. Louis University | Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension |
| DE69132531T2 (de) | 1990-12-06 | 2001-09-13 | Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) | Verbindungen und ihre Verwendung in einer binären Synthesestrategie |
| US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
| US5888819A (en) | 1991-03-05 | 1999-03-30 | Molecular Tool, Inc. | Method for determining nucleotide identity through primer extension |
| WO1992016657A1 (en) | 1991-03-13 | 1992-10-01 | E.I. Du Pont De Nemours And Company | Method of identifying a nucleotide present at a defined position in a nucleic acid |
| WO1992019960A1 (en) | 1991-05-09 | 1992-11-12 | Nanophore, Inc. | Methods for the electrophoretic separation of nucleic acids and other linear macromolecules in gel media with restrictive pore diameters |
| DE4119955C2 (de) | 1991-06-18 | 2000-05-31 | Danfoss As | Miniatur-Betätigungselement |
| US5164558A (en) | 1991-07-05 | 1992-11-17 | Massachusetts Institute Of Technology | Micromachined threshold pressure switch and method of manufacture |
| JP3328300B2 (ja) | 1991-07-18 | 2002-09-24 | アイシン精機株式会社 | 流体制御装置 |
| US5307186A (en) | 1991-08-09 | 1994-04-26 | Sharp Kabushiki Kaisha | Liquid crystal light valve having capability of providing high-contrast image |
| DE4143343C2 (de) | 1991-09-11 | 1994-09-22 | Fraunhofer Ges Forschung | Mikrominiaturisierte, elektrostatisch betriebene Mikromembranpumpe |
| US5265327A (en) | 1991-09-13 | 1993-11-30 | Faris Sadeg M | Microchannel plate technology |
| US6569382B1 (en) | 1991-11-07 | 2003-05-27 | Nanogen, Inc. | Methods apparatus for the electronic, homogeneous assembly and fabrication of devices |
| US5558998A (en) | 1992-02-25 | 1996-09-24 | The Regents Of The Univ. Of California | DNA fragment sizing and sorting by laser-induced fluorescence |
| JP2740705B2 (ja) | 1992-04-08 | 1998-04-15 | 本田技研工業株式会社 | 流体式トルクコンバータの特性シミュレーション方法および装置 |
| US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| US5498392A (en) | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| DE4220077A1 (de) | 1992-06-19 | 1993-12-23 | Bosch Gmbh Robert | Mikropumpe |
| US5710028A (en) | 1992-07-02 | 1998-01-20 | Eyal; Nurit | Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor |
| US5629147A (en) | 1992-07-17 | 1997-05-13 | Aprogenex, Inc. | Enriching and identifying fetal cells in maternal blood for in situ hybridization |
| US5364742A (en) | 1992-09-21 | 1994-11-15 | International Business Machines Corporation | Micro-miniature structures and method of fabrication thereof |
| DE69327389T2 (de) | 1992-10-29 | 2000-06-15 | Altera Corp., San Jose | Verfahren zum Prüfen von Entwürfen für programmierbare Logikschaltungen |
| US5477474A (en) | 1992-10-29 | 1995-12-19 | Altera Corporation | Computer logic simulation with dynamic modeling |
| JP2812629B2 (ja) | 1992-11-25 | 1998-10-22 | 宇宙開発事業団 | 結晶成長セル |
| US5290240A (en) | 1993-02-03 | 1994-03-01 | Pharmetrix Corporation | Electrochemical controlled dispensing assembly and method for selective and controlled delivery of a dispensing fluid |
| US5400741A (en) | 1993-05-21 | 1995-03-28 | Medical Foundation Of Buffalo, Inc. | Device for growing crystals |
| US5681024A (en) | 1993-05-21 | 1997-10-28 | Fraunhofer-Gesellschaft zur Forderung der angerwanden Forschung e.V. | Microvalve |
| SE501713C2 (sv) | 1993-09-06 | 1995-05-02 | Pharmacia Biosensor Ab | Ventil av membrantyp, speciellt för vätskehanteringsblock med mikroflödeskanaler |
| US5642015A (en) | 1993-07-14 | 1997-06-24 | The University Of British Columbia | Elastomeric micro electro mechanical systems |
| US5659171A (en) | 1993-09-22 | 1997-08-19 | Northrop Grumman Corporation | Micro-miniature diaphragm pump for the low pressure pumping of gases |
| DE69415683T2 (de) | 1993-10-04 | 1999-09-09 | Res International | Mikromaschinen-fluss-schalter |
| US5512131A (en) | 1993-10-04 | 1996-04-30 | President And Fellows Of Harvard College | Formation of microstamped patterns on surfaces and derivative articles |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| CH689836A5 (fr) | 1994-01-14 | 1999-12-15 | Westonbridge Int Ltd | Micropompe. |
| EP0670489A3 (de) | 1994-03-03 | 1996-03-06 | Ciba Geigy Ag | Vorrichtung und Verfahren zur Trennung fluider Substanzen. |
| US5580523A (en) | 1994-04-01 | 1996-12-03 | Bard; Allen J. | Integrated chemical synthesizers |
| US5587081A (en) | 1994-04-26 | 1996-12-24 | Jet-Tech, Inc. | Thermophilic aerobic waste treatment process |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| DE4433894A1 (de) | 1994-09-22 | 1996-03-28 | Fraunhofer Ges Forschung | Verfahren und Vorrichtung zur Ansteuerung einer Mikropumpe |
| DE69531430T2 (de) | 1994-10-07 | 2004-07-01 | Bayer Corp. | Entlastungsventil |
| US5496009A (en) | 1994-10-07 | 1996-03-05 | Bayer Corporation | Valve |
| DE4437274C2 (de) | 1994-10-18 | 1998-11-05 | Inst Chemo Biosensorik | Analytselektiver Sensor |
| US5500071A (en) | 1994-10-19 | 1996-03-19 | Hewlett-Packard Company | Miniaturized planar columns in novel support media for liquid phase analysis |
| US5571410A (en) | 1994-10-19 | 1996-11-05 | Hewlett Packard Company | Fully integrated miniaturized planar liquid sample handling and analysis device |
| US5641400A (en) | 1994-10-19 | 1997-06-24 | Hewlett-Packard Company | Use of temperature control devices in miniaturized planar column devices and miniaturized total analysis systems |
| DE4438785C2 (de) | 1994-10-24 | 1996-11-07 | Wita Gmbh Wittmann Inst Of Tec | Mikrochemische Reaktions- und Analyseeinheit |
| US5788468A (en) | 1994-11-03 | 1998-08-04 | Memstek Products, Llc | Microfabricated fluidic devices |
| US5632876A (en) | 1995-06-06 | 1997-05-27 | David Sarnoff Research Center, Inc. | Apparatus and methods for controlling fluid flow in microchannels |
| US5665070A (en) | 1995-01-19 | 1997-09-09 | I-Flow Corporation | Infusion pump with magnetic bag compression |
| JP3094880B2 (ja) | 1995-03-01 | 2000-10-03 | 住友金属工業株式会社 | 有機化合物の結晶化制御方法およびそれに用いる結晶化制御用固体素子 |
| US5775371A (en) | 1995-03-08 | 1998-07-07 | Abbott Laboratories | Valve control |
| US5876187A (en) | 1995-03-09 | 1999-03-02 | University Of Washington | Micropumps with fixed valves |
| US5608519A (en) | 1995-03-20 | 1997-03-04 | Gourley; Paul L. | Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells |
| US5661222A (en) | 1995-04-13 | 1997-08-26 | Dentsply Research & Development Corp. | Polyvinylsiloxane impression material |
| US5757482A (en) | 1995-04-20 | 1998-05-26 | Perseptive Biosystems, Inc. | Module for optical detection in microscale fluidic analyses |
| DE19520298A1 (de) | 1995-06-02 | 1996-12-05 | Bayer Ag | Sortiervorrichtung für biologische Zellen oder Viren |
| US5716852A (en) | 1996-03-29 | 1998-02-10 | University Of Washington | Microfabricated diffusion-based chemical sensor |
| US5589136A (en) | 1995-06-20 | 1996-12-31 | Regents Of The University Of California | Silicon-based sleeve devices for chemical reactions |
| US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| CA2183478C (en) | 1995-08-17 | 2004-02-24 | Stephen A. Carter | Digital gas metering system using tri-stable and bi-stable solenoids |
| US6130098A (en) | 1995-09-15 | 2000-10-10 | The Regents Of The University Of Michigan | Moving microdroplets |
| US5726751A (en) | 1995-09-27 | 1998-03-10 | University Of Washington | Silicon microchannel optical flow cytometer |
| US5705018A (en) | 1995-12-13 | 1998-01-06 | Hartley; Frank T. | Micromachined peristaltic pump |
| AU710425B2 (en) | 1995-12-18 | 1999-09-23 | Washington University | Method for nucleic acid analysis using fluorescence resonance energy transfer |
| KR100207410B1 (ko) | 1995-12-19 | 1999-07-15 | 전주범 | 광로 조절 장치의 제조방법 |
| JP2002515738A (ja) | 1996-01-23 | 2002-05-28 | アフィメトリックス，インコーポレイティド | 核酸分析法 |
| US5863502A (en) | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
| US5660370A (en) | 1996-03-07 | 1997-08-26 | Integrated Fludics, Inc. | Valve with flexible sheet member and two port non-flexing backer member |
| US6126899A (en) | 1996-04-03 | 2000-10-03 | The Perkins-Elmer Corporation | Device for multiple analyte detection |
| US5942443A (en) | 1996-06-28 | 1999-08-24 | Caliper Technologies Corporation | High throughput screening assay systems in microscale fluidic devices |
| US5863801A (en) | 1996-06-14 | 1999-01-26 | Sarnoff Corporation | Automated nucleic acid isolation |
| US5779868A (en) | 1996-06-28 | 1998-07-14 | Caliper Technologies Corporation | Electropipettor and compensation means for electrophoretic bias |
| CN1329729C (zh) | 1996-06-28 | 2007-08-01 | 卡钳生命科学股份有限公司 | 微流体系统 |
| US6429025B1 (en) | 1996-06-28 | 2002-08-06 | Caliper Technologies Corp. | High-throughput screening assay systems in microscale fluidic devices |
| US5800690A (en) | 1996-07-03 | 1998-09-01 | Caliper Technologies Corporation | Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces |
| EP0913507A4 (en) | 1996-07-15 | 2002-03-13 | Sumitomo Metal Ind | PLANT FOR CRYSTAL GROWTH AND THE METHOD FOR CRYSTAL GROWTH USING THIS METHOD |
| US5699157A (en) | 1996-07-16 | 1997-12-16 | Caliper Technologies Corp. | Fourier detection of species migrating in a microchannel |
| US6136212A (en) | 1996-08-12 | 2000-10-24 | The Regents Of The University Of Michigan | Polymer-based micromachining for microfluidic devices |
| US5738799A (en) | 1996-09-12 | 1998-04-14 | Xerox Corporation | Method and materials for fabricating an ink-jet printhead |
| US6221654B1 (en) | 1996-09-25 | 2001-04-24 | California Institute Of Technology | Method and apparatus for analysis and sorting of polynucleotides based on size |
| US5858187A (en) | 1996-09-26 | 1999-01-12 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing electrodynamic focusing on a microchip |
| US5854684A (en) | 1996-09-26 | 1998-12-29 | Sarnoff Corporation | Massively parallel detection |
| US6056428A (en) | 1996-11-12 | 2000-05-02 | Invention Machine Corporation | Computer based system for imaging and analyzing an engineering object system and indicating values of specific design changes |
| US5971355A (en) | 1996-11-27 | 1999-10-26 | Xerox Corporation | Microdevice valve structures to fluid control |
| EP0958593A4 (en) | 1997-01-27 | 2006-08-30 | California Inst Of Techn | MICROMECHANICAL ELECTRIC PRESSURE GASKETS FOR MASS SPECTROMETRY |
| US6376971B1 (en) | 1997-02-07 | 2002-04-23 | Sri International | Electroactive polymer electrodes |
| US5904824A (en) | 1997-03-07 | 1999-05-18 | Beckman Instruments, Inc. | Microfluidic electrophoresis device |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| CN1105914C (zh) | 1997-04-25 | 2003-04-16 | 卡钳技术有限公司 | 改进了通道几何结构的微型流体装置 |
| US5863736A (en) | 1997-05-23 | 1999-01-26 | Becton, Dickinson And Company | Method, apparatus and computer program products for determining quantities of nucleic acid sequences in samples |
| US5869004A (en) | 1997-06-09 | 1999-02-09 | Caliper Technologies Corp. | Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems |
| WO1999000655A2 (en) | 1997-06-27 | 1999-01-07 | Immunetics, Inc. | Rapid flow-through binding assay apparatus and method |
| WO1999004361A1 (en) | 1997-07-16 | 1999-01-28 | Diversified Scientific, Inc. | Method for acquiring, storing and analyzing crystal images |
| US5932799A (en) | 1997-07-21 | 1999-08-03 | Ysi Incorporated | Microfluidic analyzer module |
| US6073482A (en) | 1997-07-21 | 2000-06-13 | Ysi Incorporated | Fluid flow module |
| US6375871B1 (en) | 1998-06-18 | 2002-04-23 | 3M Innovative Properties Company | Methods of manufacturing microfluidic articles |
| US5965410A (en) | 1997-09-02 | 1999-10-12 | Caliper Technologies Corp. | Electrical current for controlling fluid parameters in microchannels |
| US7214298B2 (en) * | 1997-09-23 | 2007-05-08 | California Institute Of Technology | Microfabricated cell sorter |
| US6540895B1 (en) | 1997-09-23 | 2003-04-01 | California Institute Of Technology | Microfabricated cell sorter for chemical and biological materials |
| US5836750A (en) | 1997-10-09 | 1998-11-17 | Honeywell Inc. | Electrostatically actuated mesopump having a plurality of elementary cells |
| US5842787A (en) | 1997-10-09 | 1998-12-01 | Caliper Technologies Corporation | Microfluidic systems incorporating varied channel dimensions |
| US5958694A (en) | 1997-10-16 | 1999-09-28 | Caliper Technologies Corp. | Apparatus and methods for sequencing nucleic acids in microfluidic systems |
| AU1522299A (en) | 1997-11-12 | 1999-05-31 | California Institute Of Technology | Micromachined parylene membrane valve and pump |
| US6089534A (en) | 1998-01-08 | 2000-07-18 | Xerox Corporation | Fast variable flow microelectromechanical valves |
| US6155282A (en) | 1998-01-20 | 2000-12-05 | Triconex, Incorporated | Two out of three voting solenoid arrangement |
| US6167910B1 (en) | 1998-01-20 | 2001-01-02 | Caliper Technologies Corp. | Multi-layer microfluidic devices |
| US5997961A (en) | 1998-03-06 | 1999-12-07 | Battelle Memorial Institute | Method of bonding functional surface materials to substrates and applications in microtechnology and antifouling |
| WO1999052633A1 (en) | 1998-04-14 | 1999-10-21 | Ivd Systems | Test cartridge with a single inlet port |
| US6246330B1 (en) | 1998-05-29 | 2001-06-12 | Wyn Y. Nielsen | Elimination-absorber monitoring system |
| WO2000000678A1 (en) | 1998-06-26 | 2000-01-06 | University Of Washington | Crystallization media |
| US6406921B1 (en) | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
| US6576478B1 (en) | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
| US6132685A (en) | 1998-08-10 | 2000-10-17 | Caliper Technologies Corporation | High throughput microfluidic systems and methods |
| EP1876442A3 (en) | 1998-09-17 | 2008-03-05 | Advion BioSciences, Inc. | Integrated monolithic microfabricated liquid chromatography system and method |
| US6605472B1 (en) | 1998-10-09 | 2003-08-12 | The Governors Of The University Of Alberta | Microfluidic devices connected to glass capillaries with minimal dead volume |
| US6149787A (en) | 1998-10-14 | 2000-11-21 | Caliper Technologies Corp. | External material accession systems and methods |
| RU2143343C1 (ru) | 1998-11-03 | 1999-12-27 | Самсунг Электроникс Ко., Лтд. | Микроинжектор и способ изготовления микроинжектора |
| US6541539B1 (en) | 1998-11-04 | 2003-04-01 | President And Fellows Of Harvard College | Hierarchically ordered porous oxides |
| DE60045917D1 (de) | 1999-02-23 | 2011-06-16 | Caliper Life Sciences Inc | Sequenzierung durch inkorporation |
| WO2000050871A1 (en) | 1999-02-26 | 2000-08-31 | Orchid Biosciences, Inc. | Microstructures for use in biological assays and reactions |
| US6749814B1 (en) | 1999-03-03 | 2004-06-15 | Symyx Technologies, Inc. | Chemical processing microsystems comprising parallel flow microreactors and methods for using same |
| US6500323B1 (en) | 1999-03-26 | 2002-12-31 | Caliper Technologies Corp. | Methods and software for designing microfluidic devices |
| NZ514732A (en) | 1999-04-06 | 2004-01-30 | Uab Research Foundation | Method for screening crystallization conditions in solution crystal growth |
| JP2003501156A (ja) | 1999-06-08 | 2003-01-14 | メディカル リサーチ グループ インコーポレイテッド | 埋め込み可能な注入デバイスにおける化学反応を利用した液体の注入法およびその装置 |
| US6296673B1 (en) | 1999-06-18 | 2001-10-02 | The Regents Of The University Of California | Methods and apparatus for performing array microcrystallizations |
| US6878540B2 (en) | 1999-06-25 | 2005-04-12 | Cepheid | Device for lysing cells, spores, or microorganisms |
| US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US8550119B2 (en) * | 1999-06-28 | 2013-10-08 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| IL147302A0 (en) | 1999-06-28 | 2002-08-14 | California Inst Of Techn | Microfabricated elastomeric valve and pump systems |
| US6899137B2 (en) | 1999-06-28 | 2005-05-31 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| US6929030B2 (en) | 1999-06-28 | 2005-08-16 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| US6533914B1 (en) | 1999-07-08 | 2003-03-18 | Shaorong Liu | Microfabricated injector and capillary array assembly for high-resolution and high throughput separation |
| DE19933614C1 (de) | 1999-07-17 | 2000-11-30 | Moeller Gmbh | Kontaktsystem mit einem zweiarmigen Kontaktarm |
| DE60037433T2 (de) | 1999-07-20 | 2008-12-04 | Sri International, Menlo Park | Elektroaktive Polymergeneratoren |
| KR100677860B1 (ko) | 1999-07-23 | 2007-02-05 | 보드 오브 트러스티스 오브 유니버스티 오브 일리노이즈 | 미세하게 제조되는 장치 및 그의 제조방법 |
| US6977145B2 (en) | 1999-07-28 | 2005-12-20 | Serono Genetics Institute S.A. | Method for carrying out a biochemical protocol in continuous flow in a microreactor |
| US6811608B1 (en) | 1999-08-02 | 2004-11-02 | Emerald Biostructures, Inc. | Method and system for creating a crystallization results database |
| US6866785B2 (en) | 2001-08-13 | 2005-03-15 | The Board Of Trustees Of The Leland Stanford Junior University | Photopolymerized sol-gel column and associated methods |
| US6541071B1 (en) | 2000-03-23 | 2003-04-01 | Corning Incorporated | Method for fabricating supported bilayer-lipid membranes |
| US6358387B1 (en) | 2000-03-27 | 2002-03-19 | Caliper Technologies Corporation | Ultra high throughput microfluidic analytical systems and methods |
| AU5121801A (en) | 2000-03-31 | 2001-10-15 | Micronics Inc | Protein crystallization in microfluidic structures |
| US6690437B2 (en) * | 2000-04-18 | 2004-02-10 | Semiconductor Energy Laboratory Co., Ltd. | Electro-optical device |
| US7351376B1 (en) * | 2000-06-05 | 2008-04-01 | California Institute Of Technology | Integrated active flux microfluidic devices and methods |
| WO2002000343A2 (en) | 2000-06-27 | 2002-01-03 | Fluidigm Corporation | A microfluidic design automation method and system |
| US6885982B2 (en) | 2000-06-27 | 2005-04-26 | Fluidigm Corporation | Object oriented microfluidic design method and system |
| US6627159B1 (en) | 2000-06-28 | 2003-09-30 | 3M Innovative Properties Company | Centrifugal filling of sample processing devices |
| JP3542550B2 (ja) | 2000-07-19 | 2004-07-14 | 本田技研工業株式会社 | 燃料電池用シールの成形方法 |
| US6446706B1 (en) | 2000-07-25 | 2002-09-10 | Thermal Corp. | Flexible heat pipe |
| WO2002023163A1 (en) | 2000-09-15 | 2002-03-21 | California Institute Of Technology | Microfabricated crossflow devices and methods |
| US6787339B1 (en) | 2000-10-02 | 2004-09-07 | Motorola, Inc. | Microfluidic devices having embedded metal conductors and methods of fabricating said devices |
| US6508988B1 (en) | 2000-10-03 | 2003-01-21 | California Institute Of Technology | Combinatorial synthesis system |
| US7097809B2 (en) * | 2000-10-03 | 2006-08-29 | California Institute Of Technology | Combinatorial synthesis system |
| EP1322936A2 (en) | 2000-10-03 | 2003-07-02 | California Institute Of Technology | Microfluidic devices and methods of use |
| US7232109B2 (en) | 2000-11-06 | 2007-06-19 | California Institute Of Technology | Electrostatic valves for microfluidic devices |
| WO2002060582A2 (en) | 2000-11-16 | 2002-08-08 | Fluidigm Corporation | Microfluidic devices for introducing and dispensing fluids from microfluidic systems |
| US7378280B2 (en) * | 2000-11-16 | 2008-05-27 | California Institute Of Technology | Apparatus and methods for conducting assays and high throughput screening |
| US6736978B1 (en) | 2000-12-13 | 2004-05-18 | Iowa State University Research Foundation, Inc. | Method and apparatus for magnetoresistive monitoring of analytes in flow streams |
| US6674525B2 (en) * | 2001-04-03 | 2004-01-06 | Micronics, Inc. | Split focusing cytometer |
| WO2002082047A2 (en) | 2001-04-06 | 2002-10-17 | California Institute Of Technology | High throughput screening of crystallization of materials |
| US20020164816A1 (en) | 2001-04-06 | 2002-11-07 | California Institute Of Technology | Microfluidic sample separation device |
| AU2002307152A1 (en) * | 2001-04-06 | 2002-10-21 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
| US6752922B2 (en) | 2001-04-06 | 2004-06-22 | Fluidigm Corporation | Microfluidic chromatography |
| US6677131B2 (en) | 2001-05-14 | 2004-01-13 | Corning Incorporated | Well frame including connectors for biological fluids |
| US20030049659A1 (en) * | 2001-05-29 | 2003-03-13 | Lapidus Stanley N. | Devices and methods for isolating samples into subsamples for analysis |
| US6729352B2 (en) * | 2001-06-07 | 2004-05-04 | Nanostream, Inc. | Microfluidic synthesis devices and methods |
| US6847153B1 (en) | 2001-06-13 | 2005-01-25 | The United States Of America As Represented By The Secretary Of The Navy | Polyurethane electrostriction |
| CA2492423A1 (en) | 2001-07-15 | 2004-03-18 | Keck Graduate Institute | Exponential amplification of nucleic acids using nicking agents |
| US6444318B1 (en) | 2001-07-17 | 2002-09-03 | Surmodics, Inc. | Self assembling monolayer compositions |
| US7075162B2 (en) | 2001-08-30 | 2006-07-11 | Fluidigm Corporation | Electrostatic/electrostrictive actuation of elastomer structures using compliant electrodes |
| WO2003031066A1 (en) | 2001-10-11 | 2003-04-17 | California Institute Of Technology | Devices utilizing self-assembled gel and method of manufacture |
| US6627076B2 (en) | 2001-10-19 | 2003-09-30 | Sandia National Laboratories | Compact microchannel system |
| US20030175947A1 (en) | 2001-11-05 | 2003-09-18 | Liu Robin Hui | Enhanced mixing in microfluidic devices |
| US7118910B2 (en) | 2001-11-30 | 2006-10-10 | Fluidigm Corporation | Microfluidic device and methods of using same |
| US6893613B2 (en) | 2002-01-25 | 2005-05-17 | Bristol-Myers Squibb Company | Parallel chemistry reactor with interchangeable vessel carrying inserts |
| US6662818B2 (en) | 2002-02-01 | 2003-12-16 | Perseptive Biosystems, Inc. | Programmable tracking pressure regulator for control of higher pressures in microfluidic circuits |
| US6581441B1 (en) | 2002-02-01 | 2003-06-24 | Perseptive Biosystems, Inc. | Capillary column chromatography process and system |
| US7312085B2 (en) * | 2002-04-01 | 2007-12-25 | Fluidigm Corporation | Microfluidic particle-analysis systems |
| US7059348B2 (en) | 2002-05-13 | 2006-06-13 | Fluidigm Corporation | Drug delivery system |
| US20060086309A1 (en) * | 2002-06-24 | 2006-04-27 | Fluiding Corporation | Recirculating fluidic network and methods for using the same |
| JP2006501056A (ja) * | 2002-09-25 | 2006-01-12 | カリフォルニア インスティテュート オブ テクノロジー | ミクロ流体大規模集積 |
| US7186383B2 (en) | 2002-09-27 | 2007-03-06 | Ast Management Inc. | Miniaturized fluid delivery and analysis system |
| AU2003299541A1 (en) | 2002-10-02 | 2004-05-25 | California Institute Of Technology | Microfluidic nucleic acid analysis |
| EP1567638A1 (en) | 2002-11-08 | 2005-08-31 | Irm, Llc | Apparatus and methods to process substrate surface features |
| KR20050088476A (ko) | 2002-12-30 | 2005-09-06 | 더 리전트 오브 더 유니버시티 오브 캘리포니아 | 병원균 검출과 분석을 위한 방법과 기구 |
| US7476363B2 (en) | 2003-04-03 | 2009-01-13 | Fluidigm Corporation | Microfluidic devices and methods of using same |
| US7413712B2 (en) | 2003-08-11 | 2008-08-19 | California Institute Of Technology | Microfluidic rotary flow reactor matrix |
| US7407799B2 (en) | 2004-01-16 | 2008-08-05 | California Institute Of Technology | Microfluidic chemostat |
-
2004
- 2004-04-05 AU AU2004228678A patent/AU2004228678A1/en not_active Abandoned
- 2004-04-05 CA CA2521171A patent/CA2521171C/en not_active Expired - Lifetime
- 2004-04-05 EP EP10179295.0A patent/EP2340890B1/en not_active Expired - Lifetime
- 2004-04-05 US US10/818,642 patent/US7666361B2/en not_active Expired - Lifetime
- 2004-04-05 JP JP2006509721A patent/JP5419248B2/ja not_active Expired - Lifetime
- 2004-04-05 WO PCT/US2004/010492 patent/WO2004089810A2/en not_active Ceased
- 2004-04-05 EP EP04758896.7A patent/EP1615721B1/en not_active Expired - Lifetime
-
2007
- 2007-10-30 US US11/929,625 patent/US20080230387A1/en not_active Abandoned
-
2011
- 2011-02-10 JP JP2011027942A patent/JP2011097955A/ja not_active Withdrawn
-
2014
- 2014-03-24 JP JP2014059530A patent/JP2014110815A/ja not_active Withdrawn
-
2015
- 2015-11-19 JP JP2015226281A patent/JP2016028257A/ja not_active Withdrawn
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