JP2006521807A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2006521807A5 JP2006521807A5 JP2006506517A JP2006506517A JP2006521807A5 JP 2006521807 A5 JP2006521807 A5 JP 2006521807A5 JP 2006506517 A JP2006506517 A JP 2006506517A JP 2006506517 A JP2006506517 A JP 2006506517A JP 2006521807 A5 JP2006521807 A5 JP 2006521807A5
- Authority
- JP
- Japan
- Prior art keywords
- positive
- cells
- nestin
- ncam
- neural progenitor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cells Anatomy 0.000 claims description 72
- 102000008730 Nestin Human genes 0.000 claims description 37
- 108010088225 Nestin Proteins 0.000 claims description 37
- 239000002609 media Substances 0.000 claims description 37
- 230000001537 neural Effects 0.000 claims description 35
- 102100007544 NCAM1 Human genes 0.000 claims description 29
- 108010069196 Neural Cell Adhesion Molecules Proteins 0.000 claims description 29
- 210000000130 stem cell Anatomy 0.000 claims description 25
- 210000002569 neurons Anatomy 0.000 claims description 22
- 230000004069 differentiation Effects 0.000 claims description 16
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 16
- 230000003291 dopaminomimetic Effects 0.000 claims description 12
- 108090000715 Brain-Derived Neurotrophic Factor Proteins 0.000 claims description 10
- 102000004219 Brain-Derived Neurotrophic Factor Human genes 0.000 claims description 10
- 229940077737 Brain-Derived Neurotrophic Factor Drugs 0.000 claims description 10
- 210000002242 Embryoid Bodies Anatomy 0.000 claims description 10
- 102000003974 Fibroblast Growth Factor 2 Human genes 0.000 claims description 10
- 108090000379 Fibroblast Growth Factor 2 Proteins 0.000 claims description 10
- 102000004877 Insulin Human genes 0.000 claims description 8
- 108090001061 Insulin Proteins 0.000 claims description 8
- BVTBRVFYZUCAKH-UHFFFAOYSA-L Sodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 8
- 102000004338 Transferrin Human genes 0.000 claims description 8
- 108090000901 Transferrin Proteins 0.000 claims description 8
- 239000011781 sodium selenite Substances 0.000 claims description 8
- 229960001471 sodium selenite Drugs 0.000 claims description 8
- 235000015921 sodium selenite Nutrition 0.000 claims description 8
- 239000012581 transferrin Substances 0.000 claims description 8
- 229960004308 ACETYLCYSTEINE Drugs 0.000 claims description 7
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 claims description 7
- 108090000097 Transforming growth factor beta-3 Proteins 0.000 claims description 7
- 230000000862 serotonergic Effects 0.000 claims description 7
- 101700033006 EGF Proteins 0.000 claims description 6
- 102100010813 EGF Human genes 0.000 claims description 6
- 229940116977 Epidermal Growth Factor Drugs 0.000 claims description 6
- 102000003956 Fibroblast Growth Factor 8 Human genes 0.000 claims description 6
- 108090000368 Fibroblast Growth Factor 8 Proteins 0.000 claims description 6
- KIDHWZJUCRJVML-UHFFFAOYSA-N Putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 claims description 6
- 102100008658 FN1 Human genes 0.000 claims description 5
- 108010067306 Fibronectins Proteins 0.000 claims description 5
- 102000004329 Glial cell line-derived neurotrophic factor Human genes 0.000 claims description 5
- 108090000821 Glial cell line-derived neurotrophic factor Proteins 0.000 claims description 5
- 102000003777 Interleukin-1 beta Human genes 0.000 claims description 5
- 108090000193 Interleukin-1 beta Proteins 0.000 claims description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Vitamin C Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 5
- 239000011668 ascorbic acid Substances 0.000 claims description 5
- 235000010323 ascorbic acid Nutrition 0.000 claims description 5
- 229960005070 ascorbic acid Drugs 0.000 claims description 5
- 230000037320 fibronectin Effects 0.000 claims description 5
- 239000001963 growth media Substances 0.000 claims description 5
- 210000002966 Serum Anatomy 0.000 claims description 4
- 239000007640 basal medium Substances 0.000 claims description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- CJGYSWNGNKCJSB-YVLZZHOMSA-N Bucladesine Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](OC(=O)CCC)[C@@H]2N1C(N=CN=C2NC(=O)CCC)=C2N=C1 CJGYSWNGNKCJSB-YVLZZHOMSA-N 0.000 claims description 3
- 240000001340 Gmelina philippensis Species 0.000 claims description 3
- RJKFOVLPORLFTN-STHVQZNPSA-N Progesterone Natural products O=C(C)[C@@H]1[C@@]2(C)[C@H]([C@H]3[C@@H]([C@]4(C)C(=CC(=O)CC4)CC3)CC2)CC1 RJKFOVLPORLFTN-STHVQZNPSA-N 0.000 claims description 3
- 239000005700 Putrescine Substances 0.000 claims description 3
- RJKFOVLPORLFTN-LEKSSAKUSA-N Syngestrets Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 229960003387 progesterone Drugs 0.000 claims description 3
- 239000000186 progesterone Substances 0.000 claims description 3
- 102000018233 Fibroblast growth factor family Human genes 0.000 claims description 2
- 108050007372 Fibroblast growth factor family Proteins 0.000 claims description 2
- 210000004248 Oligodendroglia Anatomy 0.000 claims description 2
- 230000001605 fetal Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 7
- 210000001671 Embryonic Stem Cells Anatomy 0.000 claims 2
- 241000288906 Primates Species 0.000 claims 2
- 238000000608 laser ablation Methods 0.000 claims 2
- 210000003061 neural cell Anatomy 0.000 claims 2
- 102100018013 SHH Human genes 0.000 claims 1
- 101700020958 SHH Proteins 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- OAICVXFJPJFONN-UHFFFAOYSA-N phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 8
- 101700037605 LIF Proteins 0.000 description 4
- 229960003638 dopamine Drugs 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 3
- HRZFUMHJMZEROT-UHFFFAOYSA-L 7681-57-4 Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 102000001389 Glial Fibrillary Acidic Protein Human genes 0.000 description 2
- 108010093505 Glial Fibrillary Acidic Protein Proteins 0.000 description 2
- 108050003490 Insulin-like growth factor Proteins 0.000 description 2
- 102000014429 Insulin-like growth factor Human genes 0.000 description 2
- 108010025020 Nerve Growth Factor Proteins 0.000 description 2
- 102000015336 Nerve Growth Factor Human genes 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N Perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- 101700038204 TGFA Proteins 0.000 description 2
- SHGAZHPCJJPHSC-NWVFGJFESA-N Tretinoin Chemical compound OC(=O)/C=C(\C)/C=C/C=C(C)C=CC1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-NWVFGJFESA-N 0.000 description 2
- 229960001727 Tretinoin Drugs 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 230000000721 bacterilogical Effects 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 238000010185 immunofluorescence analysis Methods 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 229930002330 retinoic acid Natural products 0.000 description 2
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 description 2
- 229940001584 sodium metabisulfite Drugs 0.000 description 2
- 235000010262 sodium metabisulphite Nutrition 0.000 description 2
- 229960001322 trypsin Drugs 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N 2-mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
- 210000001130 Astrocytes Anatomy 0.000 description 1
- 101700052211 BDNF Proteins 0.000 description 1
- 102100015985 BDNF Human genes 0.000 description 1
- 101700000123 BMP2 Proteins 0.000 description 1
- 102100005377 BMP2 Human genes 0.000 description 1
- 101700005069 BMP4 Proteins 0.000 description 1
- 102100015886 BMP4 Human genes 0.000 description 1
- 102000000131 Beta tubulin Human genes 0.000 description 1
- 108050008483 Beta tubulin Proteins 0.000 description 1
- 210000000988 Bone and Bones Anatomy 0.000 description 1
- 102100007493 CNTF Human genes 0.000 description 1
- 108010005939 Ciliary Neurotrophic Factor Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102100007406 FGF4 Human genes 0.000 description 1
- 101700036125 FGF4 Proteins 0.000 description 1
- 108010014172 Factor V Proteins 0.000 description 1
- 102000016970 Follistatin Human genes 0.000 description 1
- 108010014612 Follistatin Proteins 0.000 description 1
- BTCSSZJGUNDROE-UHFFFAOYSA-N GABA Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 1
- 101700016442 GDF5 Proteins 0.000 description 1
- 108060008201 GDNF Proteins 0.000 description 1
- 102100000369 GDNF Human genes 0.000 description 1
- 229940049906 Glutamate Drugs 0.000 description 1
- 229960002989 Glutamic Acid Drugs 0.000 description 1
- 239000012593 Hanks’ Balanced Salt Solution Substances 0.000 description 1
- 102000003693 Hedgehog Proteins Human genes 0.000 description 1
- 108090000031 Hedgehog Proteins Proteins 0.000 description 1
- 108090000177 Interleukin-11 Proteins 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- 101710028765 KITLG Proteins 0.000 description 1
- 102100004938 MAP2 Human genes 0.000 description 1
- 101700017358 MAP2 Proteins 0.000 description 1
- 101710039851 METAP1D Proteins 0.000 description 1
- 101710012506 METAP2 Proteins 0.000 description 1
- 102000016776 Midkine Human genes 0.000 description 1
- 108010092801 Midkine Proteins 0.000 description 1
- 229940053128 Nerve Growth Factor Drugs 0.000 description 1
- 229940032018 Neurotrophin 3 Drugs 0.000 description 1
- 108090000742 Neurotrophin-3 Proteins 0.000 description 1
- 102000004230 Neurotrophin-3 Human genes 0.000 description 1
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 1
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 1
- 108010078762 Protein Precursors Proteins 0.000 description 1
- 102000014961 Protein Precursors Human genes 0.000 description 1
- 229940076279 Serotonin Drugs 0.000 description 1
- 102100014223 TGFA Human genes 0.000 description 1
- 102100002458 TGFB3 Human genes 0.000 description 1
- 102000006747 Transforming growth factor alpha Human genes 0.000 description 1
- 229960003692 aminobutyric acid Drugs 0.000 description 1
- 102000004965 antibodies Human genes 0.000 description 1
- 108090001123 antibodies Proteins 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000038129 antigens Human genes 0.000 description 1
- 108091007172 antigens Proteins 0.000 description 1
- 230000003078 antioxidant Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960005263 bucladesine Drugs 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000003834 intracellular Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000921 morphogenic Effects 0.000 description 1
- 108091005284 noggin protein Proteins 0.000 description 1
- 102000008872 noggin protein Human genes 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 235000011007 phosphoric acid Nutrition 0.000 description 1
- OZAIFHULBGXAKX-UHFFFAOYSA-N precursor Substances N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000004017 serum-free culture media Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000004936 stimulating Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 210000001519 tissues Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN269/MUM/2003 | 2003-03-12 | ||
IN269MU2003 | 2003-03-12 | ||
US52004803P | 2003-11-14 | 2003-11-14 | |
US60/520,048 | 2003-11-14 | ||
PCT/IB2004/001246 WO2004081172A2 (fr) | 2003-03-12 | 2004-03-11 | Derivation de neurones dopaminergiques a differentiation terminale a partir de cellules souches embryonnaires humaines |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2010035457A Division JP2010187669A (ja) | 2003-03-12 | 2010-02-19 | ヒト胚幹細胞由来の最終分化ドーパミン作動性神経細胞の誘導 |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2006521807A JP2006521807A (ja) | 2006-09-28 |
JP2006521807A5 true JP2006521807A5 (fr) | 2010-04-08 |
JP4900587B2 JP4900587B2 (ja) | 2012-03-21 |
Family
ID=34074054
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006506517A Expired - Fee Related JP4900587B2 (ja) | 2003-03-12 | 2004-03-11 | ヒト胚幹細胞由来の最終分化ドーパミン作動性神経細胞の誘導 |
Country Status (3)
Country | Link |
---|---|
JP (1) | JP4900587B2 (fr) |
CN (1) | CN1894401B (fr) |
ZA (1) | ZA200401646B (fr) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPWO2009148170A1 (ja) | 2008-06-06 | 2011-11-04 | 独立行政法人理化学研究所 | 幹細胞の培養方法 |
WO2010046136A1 (fr) * | 2008-10-24 | 2010-04-29 | B.R.A.H.M.S. Ag | Pronostic et évaluation des risques chez les patients frappés par une crise cardiaque par détermination du niveau de peptides marqueurs |
CN101824399B (zh) * | 2009-03-05 | 2012-05-30 | 中日友好医院 | 从人羊膜上皮细胞中诱导分化出多巴胺能神经元的方法及分离所得神经元的用途 |
CN101824398B (zh) * | 2009-03-05 | 2012-08-08 | 中日友好医院 | 人羊膜上皮细胞与神经干细胞共培养诱导分化多巴胺能神经元的方法 |
DK2496688T3 (en) | 2009-11-05 | 2017-04-03 | Riken | Method of Inducing Differentiation in Cultured Stem Cells |
JP5846608B2 (ja) * | 2010-03-03 | 2016-01-20 | 国立大学法人京都大学 | iPS細胞由来の神経細胞を用いた蛋白質ミスフォールディング病の診断方法 |
ES2779453T3 (es) * | 2011-11-04 | 2020-08-17 | Memorial Sloan Kettering Cancer Center | Neuronas de dopamina (DA) del mesencéfalo para injerto |
KR102073111B1 (ko) * | 2012-10-16 | 2020-02-04 | 메르츠 파마 게엠베하 운트 코. 카가아 | 신경독소 폴리펩티드의 생물학적 활성의 결정을 위한 세포 테스트 시스템 |
WO2015034012A1 (fr) * | 2013-09-05 | 2015-03-12 | 国立大学法人京都大学 | Nouveau procédé pour l'induction de cellules précurseurs neurales produisant de la dopamine |
JP6680681B2 (ja) * | 2014-09-08 | 2020-04-15 | 国立研究開発法人理化学研究所 | 小脳前駆組織の製造方法 |
CN105087475B (zh) * | 2015-09-17 | 2018-12-04 | 广州赛莱拉干细胞科技股份有限公司 | 一种细胞培养液及其应用以及诱导牙髓干细胞向神经样细胞分化的方法 |
AU2016335563B2 (en) * | 2015-10-08 | 2022-10-27 | Neurona Therapeutics Inc. | Neural precursor cell populations and uses thereof |
KR102312123B1 (ko) | 2016-04-22 | 2021-10-13 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 도파민 생산 신경전구세포의 제조방법 |
FR3058892B1 (fr) * | 2016-11-23 | 2021-04-09 | Univ Bordeaux | Unite de tissu neural et utilisation d'une telle unite pour l'implantation dans le systeme nerveux d'un mammifere |
US12053492B2 (en) * | 2016-12-12 | 2024-08-06 | Health And Biotech France (H & B France) | Perinatal tissue derived mesenchymal stem cells: method of preparation and uses thereof |
JPWO2020054647A1 (ja) * | 2018-09-14 | 2021-11-11 | 学校法人慶應義塾 | アストロサイトの製造方法 |
CN111304167B (zh) * | 2018-12-12 | 2024-03-26 | 上海泉眼生物科技有限公司 | 人源脂肪干细胞来源的神经元前体细胞及其制备方法和应用 |
WO2020153687A1 (fr) * | 2019-01-22 | 2020-07-30 | 고려대학교 산학협력단 | Procédé basé sur la conversion directe de cellules pour la différenciation de cellules souches neurales en astrocytes |
CN114606188A (zh) * | 2019-03-06 | 2022-06-10 | 安徽中盛溯源生物科技有限公司 | 一种中脑多巴胺能神经前体细胞的制备方法及其应用 |
CN110257332A (zh) * | 2019-07-08 | 2019-09-20 | 广东省赛莱拉干细胞研究院 | 一种诱导间充质干细胞分化成为多巴胺能神经元的培养基以及方法 |
CN110669143B (zh) * | 2019-10-10 | 2021-05-14 | 中国人民解放军军事科学院军事医学研究院 | 一种短肽及其应用 |
CN114657119B (zh) * | 2022-03-16 | 2023-10-20 | 广东省农业科学院动物科学研究所 | 一种初生仔猪肠神经胶质细胞的分离和原代培养方法 |
CN115591025B (zh) * | 2022-11-09 | 2024-01-02 | 深圳先进技术研究院 | 神经调控器件、制备方法及其应用 |
WO2024113351A1 (fr) * | 2022-12-02 | 2024-06-06 | Nuwacell Biotechnologies Co., Ltd. | Milieu de culture, matrice de revêtement et procédé de maturation de cellules progénitrices dopaminergiques du mésencéphale |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6277820B1 (en) * | 1998-04-09 | 2001-08-21 | Genentech, Inc. | Method of dopaminergic and serotonergic neuron formation from neuroprogenitor cells |
EP1287116A2 (fr) * | 2000-05-17 | 2003-03-05 | Geron Corporation | Populations de progeniteurs neuronaux |
US7250294B2 (en) * | 2000-05-17 | 2007-07-31 | Geron Corporation | Screening small molecule drugs using neural cells differentiated from human embryonic stem cells |
US20030036195A1 (en) * | 2001-04-20 | 2003-02-20 | Lorenz Studer | Generation of differentiated tissue from nuclear transfer embryonic stem cells and methods of use |
-
2004
- 2004-03-01 ZA ZA200401646A patent/ZA200401646B/xx unknown
- 2004-03-11 CN CN2004800127488A patent/CN1894401B/zh not_active Expired - Fee Related
- 2004-03-11 JP JP2006506517A patent/JP4900587B2/ja not_active Expired - Fee Related
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2006521807A5 (fr) | ||
EP2470645B1 (fr) | Progéniteur rétinien humain sensiblement pur, progéniteur de cerveau antérieur, et cultures de cellules d'épithélium pigmentaire rétinien et leurs procédés de fabrication | |
KR100763477B1 (ko) | 사람 배아 줄기세포로부터 최종적으로 분화된 도파민작용성 뉴런의 유도 | |
US8597945B2 (en) | Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells | |
JP4900587B2 (ja) | ヒト胚幹細胞由来の最終分化ドーパミン作動性神経細胞の誘導 | |
US20050255589A1 (en) | Generation of neural stem cells from undifferentiated human embryonic stem cells | |
US20040247571A1 (en) | Neural cells expressing tyrosine hydroxylase | |
ZA200607978B (en) | Dopaminergic neurons derived from corneal limbus, methods of isolation and uses thereof | |
IL173889A (en) | In vitro formation of GABAERGIC neurons from embryonic stem cells and their use in the treatment of neurological disorders | |
WO2011144901A1 (fr) | Expansion et différenciation dirigée des cellules souches épidermiques de la crête neurale | |
WO2006100088A1 (fr) | Cellules souches neuronales | |
KR100931887B1 (ko) | 각막 윤부에서 유도된 다능성 배아 유사 줄기 세포, 분리 방법 및 이들의 용도 | |
US8927276B2 (en) | Ex vivo progenitor and stem cell expansion and differentiation for use in the treatment of disease of the nervous system | |
US8187875B2 (en) | Dopaminergic neurons derived from corneal limbus, methods of isolation and uses thereof | |
KR101613677B1 (ko) | 줄기 세포의 신경 전구 세포로의 분화용 조성물 및 이를 이용한 방법 | |
EP1179046B1 (fr) | Cellules precurseurs a restriction de lignage, isolees a partir du tube neural de souris et de cellules souches embryonnaires de souris | |
Umur et al. | The Differentiation of Neuronal Cells from Mouse Embryonic Stem Cells | |
CN118147072A (zh) | 一种诱导人多能干细胞分化为多巴胺能神经前体细胞的培养基及多巴胺能神经前体细胞的制备方法与应用 | |
AU765517B2 (en) | Lineage-restricted precursor cells isolated from mouse neural tube and mouse embryonic stem cells | |
AU2006235917A1 (en) | Dopaminergic neurons derived from corneal limbus, methods of isolation and uses thereof | |
Tabar et al. | Embryonic Stem Cells for Grafting in Parkinson's Disease | |
Mak et al. | High Efficiency of Neural Induction in Parkinson’s Disease-Specific Induced Pluripotent Stem Cells | |
Bentz | Brain Microenvironment and its Influence on Gene Expression and Differentiation of Murine Embryonic Stem Cells. Implications for Cell Replacement Strategies |