JP2006506332A5 - - Google Patents
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- Publication number
- JP2006506332A5 JP2006506332A5 JP2004520457A JP2004520457A JP2006506332A5 JP 2006506332 A5 JP2006506332 A5 JP 2006506332A5 JP 2004520457 A JP2004520457 A JP 2004520457A JP 2004520457 A JP2004520457 A JP 2004520457A JP 2006506332 A5 JP2006506332 A5 JP 2006506332A5
- Authority
- JP
- Japan
- Prior art keywords
- inclusion bodies
- inclusion
- interleukin
- expression product
- lipid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000003000 Inclusion Bodies Anatomy 0.000 claims 6
- 229940028885 Interleukin-4 Drugs 0.000 claims 2
- 102000004388 Interleukin-4 Human genes 0.000 claims 2
- 108090000978 Interleukin-4 Proteins 0.000 claims 2
- 150000002632 lipids Chemical class 0.000 claims 2
- 210000002421 Cell Wall Anatomy 0.000 claims 1
- 102000008300 Mutant Proteins Human genes 0.000 claims 1
- 108010021466 Mutant Proteins Proteins 0.000 claims 1
- 210000004027 cells Anatomy 0.000 claims 1
- 230000036425 denaturation Effects 0.000 claims 1
- 238000004925 denaturation Methods 0.000 claims 1
- 239000003599 detergent Substances 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 238000003259 recombinant expression Methods 0.000 claims 1
- 230000003381 solubilizing Effects 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Claims (1)
- （ａ）封入体中に発現させること、
（ｂ）細胞を分解し、封入体を分離すること、
（ｃ）そのように得られた封入体を洗浄すること、
（ｄ）変性により封入体を可溶化させること、
（ｅ）発現生成物を復元すること、および
（ｆ）発現生成物を精製すること、
を含んで成り、（ｃ）で封入体が封入体の表面に結合された脂質もしくは細胞壁断片中に含まれる脂質を効果的に可溶化させる洗浄剤を含むバッファーで洗浄されることを特徴とする、組換え体発現によるインターロイキン‐４もしくはインターロイキン‐４の突然変異蛋白質の調製法。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02015589A EP1382614A1 (en) | 2002-07-15 | 2002-07-15 | Process for the purification of interleukin-4 and its muteins |
| PCT/EP2003/007022 WO2004007549A1 (en) | 2002-07-15 | 2003-07-02 | Process for the purification of interleukin-4 and its muteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010288204A Division JP2011088922A (ja) | 2002-07-15 | 2010-12-24 | インターロイキン−４およびその突然変異蛋白質の精製法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006506332A JP2006506332A (ja) | 2006-02-23 |
| JP2006506332A5 true JP2006506332A5 (ja) | 2006-07-06 |
Family
ID=29762614
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004520457A Pending JP2006506332A (ja) | 2002-07-15 | 2003-07-02 | インターロイキン−４およびその突然変異蛋白質の精製法 |
| JP2010288204A Pending JP2011088922A (ja) | 2002-07-15 | 2010-12-24 | インターロイキン−４およびその突然変異蛋白質の精製法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010288204A Pending JP2011088922A (ja) | 2002-07-15 | 2010-12-24 | インターロイキン−４およびその突然変異蛋白質の精製法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20060134690A1 (ja) |
| EP (2) | EP1382614A1 (ja) |
| JP (2) | JP2006506332A (ja) |
| AT (1) | ATE352566T1 (ja) |
| AU (1) | AU2003244635A1 (ja) |
| DE (1) | DE60311470T2 (ja) |
| ES (1) | ES2280764T3 (ja) |
| WO (1) | WO2004007549A1 (ja) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1382614A1 (en) * | 2002-07-15 | 2004-01-21 | Bayer HealthCare AG | Process for the purification of interleukin-4 and its muteins |
| US8189621B2 (en) | 2006-05-12 | 2012-05-29 | Microsoft Corporation | Stack signaling to application with lack of requested bandwidth |
| US8144793B2 (en) | 2006-12-12 | 2012-03-27 | Microsoft Corporation | Cognitive multi-user OFDMA |
| US8268604B2 (en) * | 2007-12-20 | 2012-09-18 | Abbott Point Of Care Inc. | Compositions for forming immobilized biological layers for sensing |
| US8241697B2 (en) | 2007-12-20 | 2012-08-14 | Abbott Point Of Care Inc. | Formation of immobilized biological layers for sensing |
| KR101113495B1 (ko) | 2008-01-23 | 2012-02-29 | 한미홀딩스 주식회사 | 인간 인터페론 베타의 정제방법 |
| JP2012520326A (ja) * | 2009-03-13 | 2012-09-06 | アエロヴァンス インコーポレイティッド | 組換えタンパク質の再生法 |
| US20120108512A1 (en) * | 2009-05-07 | 2012-05-03 | Novo Nordisk Health Care Ag | Stable pharmaceutical compositions of peptide derivatized using an oxime linker |
| KR20130117760A (ko) * | 2010-08-30 | 2013-10-28 | 에프. 호프만-라 로슈 아게 | 테트라넥틴-아포지단백질 a-i 입자의 제조 방법, 이에 의해 제조된 지질 입자 및 이의 용도 |
| EP2611419A2 (en) * | 2010-08-30 | 2013-07-10 | F.Hoffmann-La Roche Ag | Method for producing a tetranectin-apolipoprotein a-1 lipid particle, the lipid particle itself and its use |
| CN103320447A (zh) * | 2013-05-20 | 2013-09-25 | 深圳市亚太兴实业有限公司 | 重组人白介素4基因、含重组人白介素4基因的工程菌发酵方法 |
| CN108753753B (zh) * | 2018-05-28 | 2022-04-22 | 中国林业科学研究院林业研究所 | Lrr受体激酶-pxy的纯化方法 |
| TW202120521A (zh) | 2019-08-16 | 2021-06-01 | 美商應用分子運輸公司 | 組合物、配方及介白素生產及純化 |
| CN116874552B (zh) * | 2023-09-08 | 2023-12-08 | 成都华任康生物科技有限公司 | 一种目标蛋白的纯化方法及其试剂盒和相关应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4137333A1 (de) * | 1991-11-13 | 1993-05-19 | W Prof Dr Sebald | Therapeutische mittel, die antagonisten oder partielle agonisten des humanen interleukin 4 sind oder diese enthalten, hil-4-mutantenproteine sowie vefahren zu deren herstellung |
| ES2199959T3 (es) * | 1993-02-04 | 2004-03-01 | Borean Pharma A/S | Procedimiento mejorado para el replegamiento de proteinas. |
| US5563057A (en) * | 1994-10-31 | 1996-10-08 | Wisconsin Alumni Research Foundation | Method for refolding misfolded enzymes with detergent and cyclodextrin |
| JP3344618B2 (ja) * | 1997-06-20 | 2002-11-11 | 株式会社エイチ・エス・ピー研究所 | シャペロン発現プラスミド |
| US5932102A (en) * | 1998-01-12 | 1999-08-03 | Schering Corporation | Immobilized metal, affinity chromatography |
| EP1382614A1 (en) * | 2002-07-15 | 2004-01-21 | Bayer HealthCare AG | Process for the purification of interleukin-4 and its muteins |
-
2002
- 2002-07-15 EP EP02015589A patent/EP1382614A1/en not_active Withdrawn
-
2003
- 2003-07-02 EP EP03738109A patent/EP1523500B1/en not_active Expired - Lifetime
- 2003-07-02 AU AU2003244635A patent/AU2003244635A1/en not_active Abandoned
- 2003-07-02 AT AT03738109T patent/ATE352566T1/de not_active IP Right Cessation
- 2003-07-02 US US10/520,883 patent/US20060134690A1/en not_active Abandoned
- 2003-07-02 JP JP2004520457A patent/JP2006506332A/ja active Pending
- 2003-07-02 DE DE60311470T patent/DE60311470T2/de not_active Expired - Lifetime
- 2003-07-02 WO PCT/EP2003/007022 patent/WO2004007549A1/en active IP Right Grant
- 2003-07-02 ES ES03738109T patent/ES2280764T3/es not_active Expired - Lifetime
-
2010
- 2010-11-12 US US12/945,829 patent/US20110060128A1/en not_active Abandoned
- 2010-12-24 JP JP2010288204A patent/JP2011088922A/ja active Pending
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