JP2006502705A - ピキアパストリス（Ｐｉｃｈｉａｐａｓｔｏｒｉｓ）におけるタンパク質グリコシル化修飾 - Google Patents

ピキアパストリス（Ｐｉｃｈｉａｐａｓｔｏｒｉｓ）におけるタンパク質グリコシル化修飾 Download PDF

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JP2006502705A
JP2006502705A JP2004516676A JP2004516676A JP2006502705A JP 2006502705 A JP2006502705 A JP 2006502705A JP 2004516676 A JP2004516676 A JP 2004516676A JP 2004516676 A JP2004516676 A JP 2004516676A JP 2006502705 A JP2006502705 A JP 2006502705A
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vector
mannosidase
methylotrophic yeast
protein
gene
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JP2006502705A5 (https=
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ローランド コントレラス、
ニコ エル． エム． カレワート、
スティーブン シー． ジェイ． ジーセンズ、
ウラジミル カイゴロドフ、
ベルベッケン ウォーター、
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フランダース インターユニバーシティ インスティテュート フォア バイオテクノロジー （ヴィーアイビー）
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Publication of JP2006502705A publication Critical patent/JP2006502705A/ja
Publication of JP2006502705A5 publication Critical patent/JP2006502705A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01114—Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase (3.2.1.114)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01024—Alpha-mannosidase (3.2.1.24)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2004516676A 2002-06-26 2003-06-25 ピキアパストリス（Ｐｉｃｈｉａｐａｓｔｏｒｉｓ）におけるタンパク質グリコシル化修飾 Pending JP2006502705A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/185,475 US7252933B2 (en) 2002-06-26 2002-06-26 Protein glycosylation modification in methylotrophic yeast
PCT/EP2003/006711 WO2004003194A2 (en) 2002-06-26 2003-06-25 Protein glycosylation modification in pichia pastoris

Publications (2)

Publication Number Publication Date
JP2006502705A true JP2006502705A (ja) 2006-01-26
JP2006502705A5 JP2006502705A5 (https=) 2006-04-27

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JP2004516676A Pending JP2006502705A (ja) 2002-06-26 2003-06-25 ピキアパストリス（Ｐｉｃｈｉａｐａｓｔｏｒｉｓ）におけるタンパク質グリコシル化修飾

Country Status (10)

Country Link
US (2) US7252933B2 (https=)
EP (2) EP2302047A1 (https=)
JP (1) JP2006502705A (https=)
KR (1) KR101047167B1 (https=)
AU (1) AU2003238051B2 (https=)
CA (1) CA2490268C (https=)
IL (2) IL165717A0 (https=)
MX (1) MXPA04012983A (https=)
NO (1) NO20050410L (https=)
WO (1) WO2004003194A2 (https=)

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JP2010517520A (ja) * 2007-02-02 2010-05-27 グリコド 均質な糖タンパク質の生産のための遺伝的に修飾された酵母

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US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7598055B2 (en) 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
EP1294910B1 (en) * 2000-06-30 2008-11-19 VIB vzw Protein glycosylation modification in pichia pastoris
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
US7507573B2 (en) * 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
WO2006026992A1 (en) * 2004-09-07 2006-03-16 Novozymes A/S Altered structure of n-glycans in a fungus
EP1934605B1 (en) * 2005-09-22 2014-03-26 Prosci Incorporated Glycosylated polypeptides produced in yeast mutants and methods of use thereof
KR101105718B1 (ko) 2005-10-27 2012-01-17 한국생명공학연구원 돌리칠 포스페이트 만노스 의존알파-1,3-만노실트랜스퍼라제를 코딩하는 한세눌라폴리모르파 기원의 신규 유전자와 상기 유전자가 결손된한세눌라 폴리모르파 변이주를 이용한 재조합 당단백질생산 방법
CA2887752C (en) * 2007-04-03 2020-03-24 Vib Vzw Glycosylation of molecules
EP2435476A4 (en) * 2009-05-27 2013-04-17 Synageva Biopharma Corp ANTIBODIES OBTAINED FROM BIRDS
KR102000383B1 (ko) 2009-09-29 2019-07-15 유니버시테이트 젠트 만노스-1-포스포-6-만노스 결합의 포스포-6-만노스로의 가수분해
EP2494050A4 (en) * 2009-10-30 2013-10-30 Merck Sharp & Dohme GRANULOCYTIC COLONY STIMULATING FACTOR MADE IN GLYCOENGINEERED PICHIA PASTORIS
US20130011875A1 (en) * 2009-10-30 2013-01-10 Merck Sharpe & Dohme Corp Methods for the production of recombinant proteins with improved secretion efficiencies
WO2011061629A2 (en) * 2009-11-19 2011-05-26 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
US9347050B2 (en) 2010-09-29 2016-05-24 Oxyrane Uk Limited Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated N-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
CA2812872C (en) 2010-09-29 2021-08-03 Oxyrane Uk Limited De-mannosylation of phosphorylated n-glycans
WO2012170292A1 (en) * 2011-06-07 2012-12-13 Merck Sharp & Dohme Corp. Modulation of metabolic pathways for improving bioprocess performance and secreted protein productivity of yeast
CN104024849A (zh) 2011-08-12 2014-09-03 生命科技公司 用于高通量聚糖分析的装置、方法、计算机程序产品和试剂盒
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US9764006B2 (en) 2012-12-10 2017-09-19 The General Hospital Corporation Bivalent IL-2 fusion toxins
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JP2021534763A (ja) * 2018-08-21 2021-12-16 クララ フーズ カンパニー 微生物におけるタンパク質のグリコシル化の修飾
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Also Published As

Publication number Publication date
NO20050410L (no) 2005-03-23
US20080009037A1 (en) 2008-01-10
IL165717A (en) 2011-06-30
US8883445B2 (en) 2014-11-11
IL165717A0 (en) 2006-01-15
KR20050054875A (ko) 2005-06-10
EP1516048A2 (en) 2005-03-23
CA2490268C (en) 2012-06-05
US20040018588A1 (en) 2004-01-29
US7252933B2 (en) 2007-08-07
KR101047167B1 (ko) 2011-07-07
EP2302047A1 (en) 2011-03-30
AU2003238051B2 (en) 2008-03-13
AU2003238051A1 (en) 2004-01-19
WO2004003194A3 (en) 2004-04-22
MXPA04012983A (es) 2005-05-16
WO2004003194A2 (en) 2004-01-08
CA2490268A1 (en) 2004-01-08

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