AU2003238051B2 - Protein glycosylation modification in pichia pastoris - Google Patents

Protein glycosylation modification in pichia pastoris Download PDF

Info

Publication number
AU2003238051B2
AU2003238051B2 AU2003238051A AU2003238051A AU2003238051B2 AU 2003238051 B2 AU2003238051 B2 AU 2003238051B2 AU 2003238051 A AU2003238051 A AU 2003238051A AU 2003238051 A AU2003238051 A AU 2003238051A AU 2003238051 B2 AU2003238051 B2 AU 2003238051B2
Authority
AU
Australia
Prior art keywords
strain
mannosidase
gene
vector
glcnac
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU2003238051A
Other languages
English (en)
Other versions
AU2003238051A1 (en
Inventor
Nico L. M. Callewaert
Roland Contreras
Steven C. J. Geysens
Vladimir Kaigorodov
Vervecken Wouter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universiteit Gent
Vlaams Instituut voor Biotechnologie VIB
Research Corp Technologies Inc
Original Assignee
Universiteit Gent
Vlaams Instituut voor Biotechnologie VIB
Research Corp Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiteit Gent, Vlaams Instituut voor Biotechnologie VIB, Research Corp Technologies Inc filed Critical Universiteit Gent
Publication of AU2003238051A1 publication Critical patent/AU2003238051A1/en
Application granted granted Critical
Publication of AU2003238051B2 publication Critical patent/AU2003238051B2/en
Assigned to RESEARCH CORPORATION TECHNOLOGIES, INC., VIB, VZW, UNIVERSITEIT GENT reassignment RESEARCH CORPORATION TECHNOLOGIES, INC. Request for Assignment Assignors: FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01114—Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase (3.2.1.114)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01024—Alpha-mannosidase (3.2.1.24)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AU2003238051A 2002-06-26 2003-06-25 Protein glycosylation modification in pichia pastoris Expired AU2003238051B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/185,475 US7252933B2 (en) 2002-06-26 2002-06-26 Protein glycosylation modification in methylotrophic yeast
US10/185,475 2002-06-26
PCT/EP2003/006711 WO2004003194A2 (en) 2002-06-26 2003-06-25 Protein glycosylation modification in pichia pastoris

Publications (2)

Publication Number Publication Date
AU2003238051A1 AU2003238051A1 (en) 2004-01-19
AU2003238051B2 true AU2003238051B2 (en) 2008-03-13

Family

ID=29999263

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003238051A Expired AU2003238051B2 (en) 2002-06-26 2003-06-25 Protein glycosylation modification in pichia pastoris

Country Status (10)

Country Link
US (2) US7252933B2 (https=)
EP (2) EP2302047A1 (https=)
JP (1) JP2006502705A (https=)
KR (1) KR101047167B1 (https=)
AU (1) AU2003238051B2 (https=)
CA (1) CA2490268C (https=)
IL (2) IL165717A0 (https=)
MX (1) MXPA04012983A (https=)
NO (1) NO20050410L (https=)
WO (1) WO2004003194A2 (https=)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7625756B2 (en) * 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7598055B2 (en) 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
EP1294910B1 (en) * 2000-06-30 2008-11-19 VIB vzw Protein glycosylation modification in pichia pastoris
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
US7507573B2 (en) * 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
WO2006026992A1 (en) * 2004-09-07 2006-03-16 Novozymes A/S Altered structure of n-glycans in a fungus
EP1934605B1 (en) * 2005-09-22 2014-03-26 Prosci Incorporated Glycosylated polypeptides produced in yeast mutants and methods of use thereof
KR101105718B1 (ko) 2005-10-27 2012-01-17 한국생명공학연구원 돌리칠 포스페이트 만노스 의존알파-1,3-만노실트랜스퍼라제를 코딩하는 한세눌라폴리모르파 기원의 신규 유전자와 상기 유전자가 결손된한세눌라 폴리모르파 변이주를 이용한 재조합 당단백질생산 방법
FR2912154B1 (fr) * 2007-02-02 2012-11-02 Glycode Levures genetiquement modifiees pour la production de glycoproteines homogenes
CA2887752C (en) * 2007-04-03 2020-03-24 Vib Vzw Glycosylation of molecules
EP2435476A4 (en) * 2009-05-27 2013-04-17 Synageva Biopharma Corp ANTIBODIES OBTAINED FROM BIRDS
KR102000383B1 (ko) 2009-09-29 2019-07-15 유니버시테이트 젠트 만노스-1-포스포-6-만노스 결합의 포스포-6-만노스로의 가수분해
EP2494050A4 (en) * 2009-10-30 2013-10-30 Merck Sharp & Dohme GRANULOCYTIC COLONY STIMULATING FACTOR MADE IN GLYCOENGINEERED PICHIA PASTORIS
US20130011875A1 (en) * 2009-10-30 2013-01-10 Merck Sharpe & Dohme Corp Methods for the production of recombinant proteins with improved secretion efficiencies
WO2011061629A2 (en) * 2009-11-19 2011-05-26 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
US9347050B2 (en) 2010-09-29 2016-05-24 Oxyrane Uk Limited Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated N-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
CA2812872C (en) 2010-09-29 2021-08-03 Oxyrane Uk Limited De-mannosylation of phosphorylated n-glycans
WO2012170292A1 (en) * 2011-06-07 2012-12-13 Merck Sharp & Dohme Corp. Modulation of metabolic pathways for improving bioprocess performance and secreted protein productivity of yeast
CN104024849A (zh) 2011-08-12 2014-09-03 生命科技公司 用于高通量聚糖分析的装置、方法、计算机程序产品和试剂盒
EP3628326B1 (en) 2012-03-15 2024-02-28 Oxyrane UK Limited Methods and materials for treatment of pompe's disease
US9764006B2 (en) 2012-12-10 2017-09-19 The General Hospital Corporation Bivalent IL-2 fusion toxins
US9944689B2 (en) 2013-03-07 2018-04-17 The General Hospital Corporation Human CTLA4 mutants and use thereof
WO2015100058A1 (en) * 2013-12-23 2015-07-02 Research Corporation Technologies, Inc. Pichia pastoris surface display system
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
JP2021534763A (ja) * 2018-08-21 2021-12-16 クララ フーズ カンパニー 微生物におけるタンパク質のグリコシル化の修飾
EP3931330A4 (en) 2019-02-25 2023-03-15 Ginkgo Bioworks, Inc. BIOSYNTHESIS OF CANNABINOIDS AND CANNABINOID PRECURSORS
KR20220034848A (ko) 2019-07-11 2022-03-18 클라라 푸드즈 컴퍼니 단백질 조성물 및 이의 섭취 가능한 제품
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
WO2022108839A1 (en) * 2020-11-17 2022-05-27 Ginkgo Bioworks, Inc. Synthetic methanol inducible promoters and uses thereof
US11639515B2 (en) * 2021-05-21 2023-05-02 Taixing Dongsheng Bio-Tech Co., Ltd Genetically engineered strain for producing porcine myoglobin and food-grade fermentation and purification thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009694A2 (en) * 1990-11-30 1992-06-11 Hsc Research And Development Limited Partnership CLONING OF UDP-N-ACETYLGLUCOSAMINE:α-3-D-MANNOSIDE β-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE I
US5834251A (en) * 1994-12-30 1998-11-10 Alko Group Ltd. Methods of modifying carbohydrate moieties
WO2002000879A2 (en) * 2000-06-28 2002-01-03 Glycofi, Inc. Methods for producing modified glycoproteins

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US137134A (en) * 1873-03-25 Improvement in locomotive spark-arresters
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4837148A (en) 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4882279A (en) 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4812405A (en) 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
US5080522A (en) 1988-12-13 1992-01-14 Shafirkin David I Device for locking and sealing of objects
US5358967A (en) 1993-07-06 1994-10-25 Rohm And Haas Company Wool protecting compositons and methods
DE60032370T2 (de) 1999-08-19 2007-10-11 Kirin Beer K.K. Hefevarianten und verfahren zur herstellung von glykoprotein enthaltenden zuckerketten vom säugetiertyp
EP1294910B1 (en) 2000-06-30 2008-11-19 VIB vzw Protein glycosylation modification in pichia pastoris

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009694A2 (en) * 1990-11-30 1992-06-11 Hsc Research And Development Limited Partnership CLONING OF UDP-N-ACETYLGLUCOSAMINE:α-3-D-MANNOSIDE β-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE I
US5834251A (en) * 1994-12-30 1998-11-10 Alko Group Ltd. Methods of modifying carbohydrate moieties
WO2002000879A2 (en) * 2000-06-28 2002-01-03 Glycofi, Inc. Methods for producing modified glycoproteins

Also Published As

Publication number Publication date
NO20050410L (no) 2005-03-23
US20080009037A1 (en) 2008-01-10
IL165717A (en) 2011-06-30
US8883445B2 (en) 2014-11-11
IL165717A0 (en) 2006-01-15
KR20050054875A (ko) 2005-06-10
EP1516048A2 (en) 2005-03-23
CA2490268C (en) 2012-06-05
US20040018588A1 (en) 2004-01-29
US7252933B2 (en) 2007-08-07
JP2006502705A (ja) 2006-01-26
KR101047167B1 (ko) 2011-07-07
EP2302047A1 (en) 2011-03-30
AU2003238051A1 (en) 2004-01-19
WO2004003194A3 (en) 2004-04-22
MXPA04012983A (es) 2005-05-16
WO2004003194A2 (en) 2004-01-08
CA2490268A1 (en) 2004-01-08

Similar Documents

Publication Publication Date Title
AU2003238051B2 (en) Protein glycosylation modification in pichia pastoris
US8663971B2 (en) Protein glycosylation modification in methylotrophic yeast
AU2001277658A1 (en) Protein glycosylation modification in pichia pastoris
US8058053B2 (en) Modification of protein glycosylation in methylotrophic yeast
US7629163B2 (en) Methods for producing modified glycoproteins
US20090087882A1 (en) Protein secretion in eukaryotic cells
JP2012152211A (ja) 糖鎖改変酵母及びそれを用いた糖タンパク質の製造方法
US8003349B2 (en) YLMPO1 gene derived from yarrowia lipolytica and a process for preparing a glycoprotein not being mannosylphosphorylated by using a mutated yarrowia lipolytica in which YLMPO1 gene is disrupted
KR20040004089A (ko) 당외쇄 합성 결손 한세눌라 폴리모르파 변이주 및 그를이용한 재조합 당단백질의 제조 방법

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: RESEARCH CORPORATION TECHNOLOGIES, INC.; VIB, VZW;

Free format text: FORMER OWNER WAS: FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY

MK14 Patent ceased section 143(a) (annual fees not paid) or expired