JP2006296438A5 - - Google Patents

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Publication number
JP2006296438A5
JP2006296438A5 JP2006201808A JP2006201808A JP2006296438A5 JP 2006296438 A5 JP2006296438 A5 JP 2006296438A5 JP 2006201808 A JP2006201808 A JP 2006201808A JP 2006201808 A JP2006201808 A JP 2006201808A JP 2006296438 A5 JP2006296438 A5 JP 2006296438A5
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JP
Japan
Prior art keywords
product
gene
dna construct
host cell
eukaryotic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2006201808A
Other languages
English (en)
Japanese (ja)
Other versions
JP4528940B2 (ja
JP2006296438A (ja
Filing date
Publication date
Priority claimed from US08/286,740 external-priority patent/US5561053A/en
Application filed filed Critical
Publication of JP2006296438A publication Critical patent/JP2006296438A/ja
Publication of JP2006296438A5 publication Critical patent/JP2006296438A5/ja
Application granted granted Critical
Publication of JP4528940B2 publication Critical patent/JP4528940B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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JP2006201808A 1994-08-05 2006-07-25 高発現の宿主細胞を選択する方法 Expired - Lifetime JP4528940B2 (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/286,740 US5561053A (en) 1994-08-05 1994-08-05 Method for selecting high-expressing host cells

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP8506644A Division JPH10503376A (ja) 1994-08-05 1995-07-28 高発現の宿主細胞を選択する方法

Publications (3)

Publication Number Publication Date
JP2006296438A JP2006296438A (ja) 2006-11-02
JP2006296438A5 true JP2006296438A5 (https=) 2008-10-23
JP4528940B2 JP4528940B2 (ja) 2010-08-25

Family

ID=23099965

Family Applications (2)

Application Number Title Priority Date Filing Date
JP8506644A Withdrawn JPH10503376A (ja) 1994-08-05 1995-07-28 高発現の宿主細胞を選択する方法
JP2006201808A Expired - Lifetime JP4528940B2 (ja) 1994-08-05 2006-07-25 高発現の宿主細胞を選択する方法

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP8506644A Withdrawn JPH10503376A (ja) 1994-08-05 1995-07-28 高発現の宿主細胞を選択する方法

Country Status (13)

Country Link
US (1) US5561053A (https=)
EP (1) EP0770136B1 (https=)
JP (2) JPH10503376A (https=)
AT (1) ATE353970T1 (https=)
AU (1) AU704408B2 (https=)
CA (1) CA2195303C (https=)
DE (1) DE69535389T2 (https=)
DK (1) DK0770136T3 (https=)
ES (1) ES2282994T3 (https=)
IL (1) IL114820A0 (https=)
MX (1) MX9700852A (https=)
PT (1) PT770136E (https=)
WO (1) WO1996004391A1 (https=)

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US20030054400A1 (en) 1997-09-17 2003-03-20 Genentech, Inc. Secreted and transmembrane polypeptides and nucleic acids encoding the same
US7169906B2 (en) * 1997-09-17 2007-01-30 Genentech, Inc. PRO211 polypeptides
US6974689B1 (en) * 1997-09-18 2005-12-13 Genentech, Inc. Nucleic acid encoding PRO211 polypeptides
CN100582230C (zh) * 1997-09-26 2010-01-20 阿瑟西斯公司 通过载体构建体与细胞dna的非同源重组来表达内源基因
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US7316923B1 (en) 1997-09-26 2008-01-08 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
WO2000056914A1 (en) * 1999-03-24 2000-09-28 Gene Therapy Systems, Inc. Method for generating transcriptionally active dna fragments
DK1196566T3 (da) * 1999-07-12 2006-06-06 Genentech Inc Ekspressionsvektorer og -metoder
US20050005310A1 (en) * 1999-07-12 2005-01-06 Genentech, Inc. Expression vectors and methods
GB2355010A (en) * 1999-10-04 2001-04-11 Jan Joris Brosens Use of cell-specific splicing in gene therapy
US20020165175A1 (en) * 2001-04-17 2002-11-07 Xiaowu Liang Fast and enzymeless cloning of nucleic acid fragments
PL368972A1 (en) 2001-08-29 2005-04-04 Genentech, Inc. Bv8 nucleic acids and polypeptides with mitogenic activity
US20030157641A1 (en) * 2001-11-16 2003-08-21 Idec Pharmaceuticals Corporation Polycistronic expression of antibodies
EP2022799A2 (en) 2001-11-16 2009-02-11 Biogen Idec Inc. Polycistronic expression of antibodies
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ATE354645T1 (de) * 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
JP4912144B2 (ja) 2003-03-12 2012-04-11 ジェネンテック， インコーポレイテッド 造血促進のためのｂｖ８及び／又はｅｇ−ｖｅｇｆの使用
AU2005222963B2 (en) * 2004-03-15 2009-10-29 Biogen Idec Ma Inc. Methods and constructs for expressing polypeptide multimers in eukaryotic cells using alternative splicing
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) * 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060172382A1 (en) * 2004-11-08 2006-08-03 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP1809750B1 (en) 2004-11-08 2012-03-21 ChromaGenics B.V. Selection of host cells expressing protein at high levels
US7968700B2 (en) * 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof
US8852928B2 (en) * 2007-07-06 2014-10-07 Temasek Life Sciences Laboratory Limited LexA-based chemical-inducible gene expression system for use in transgenic animals
JP4976502B2 (ja) 2007-10-15 2012-07-18 中外製薬株式会社 抗体の製造方法
EP2443239B1 (en) 2009-06-15 2015-11-25 Cellagenics B.V. Novel stringent selectable markers
CN103038352B (zh) 2010-06-15 2015-12-16 萨拉基尼克有限公司 用于增强基因表达的新型基因间元件
CN103180443B (zh) 2010-09-01 2016-01-13 萨拉基尼克有限公司 用于增强基因表达的来自核糖体蛋白启动子的核酸片段
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
EP3578660A1 (en) 2012-07-05 2019-12-11 F. Hoffmann-La Roche AG Expression and secretion system
HK1211981A1 (en) 2012-09-02 2016-06-03 Abbvie Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
BR112015025041A2 (pt) * 2013-03-30 2017-09-12 Usha Biotech Ltd métodos e conceitos para expressar proteínas biologicamente ativas em células de mamíferos
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
WO2020020471A1 (en) 2018-07-27 2020-01-30 Umc Utrecht Holding B.V. Ascl2-responsive reporters for labeling of intestinal stem cell activity

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US4634665A (en) * 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
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FI86885C (fi) * 1984-04-20 1992-10-26 Genentech Inc Foerfarande foer framstaellning av human rekombinantfaktor viii och nukleinsyrasekvenser och vektorer anvaend daertill
CA1341228C (en) * 1985-06-27 2001-05-15 Mark J. Murray Expression of protein c
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US5043270A (en) * 1989-03-31 1991-08-27 The Board Of Trustees Of The Leland Stanford Junior University Intronic overexpression vectors
CA2104598C (en) * 1991-03-29 2003-01-07 Justus B. Cohen Methods for selection of recombinant host cells expressing high levels of a desired protein
MX9305183A (es) * 1992-08-27 1994-05-31 Us Agriculture Intron portatil, molecula de adn genomico viral, virus vivo y vacuna que los contiene y metodo para su obtencion.

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