JP2006280277A - 核酸の抽出方法 - Google Patents
核酸の抽出方法 Download PDFInfo
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- JP2006280277A JP2006280277A JP2005104816A JP2005104816A JP2006280277A JP 2006280277 A JP2006280277 A JP 2006280277A JP 2005104816 A JP2005104816 A JP 2005104816A JP 2005104816 A JP2005104816 A JP 2005104816A JP 2006280277 A JP2006280277 A JP 2006280277A
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- nucleic acid
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 128
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 126
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 126
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000002245 particle Substances 0.000 claims abstract description 74
- 125000003277 amino group Chemical group 0.000 claims abstract description 36
- 239000000243 solution Substances 0.000 claims abstract description 30
- 150000001450 anions Chemical class 0.000 claims abstract description 25
- 239000012266 salt solution Substances 0.000 claims abstract description 24
- 238000001514 detection method Methods 0.000 claims abstract description 19
- 238000000605 extraction Methods 0.000 claims description 41
- 238000003752 polymerase chain reaction Methods 0.000 claims description 38
- 241000894006 Bacteria Species 0.000 claims description 12
- 239000011324 bead Substances 0.000 claims description 9
- 239000000696 magnetic material Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 238000003205 genotyping method Methods 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 150000002739 metals Chemical class 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 abstract description 7
- 230000010354 integration Effects 0.000 abstract 1
- 230000005291 magnetic effect Effects 0.000 description 48
- 230000001580 bacterial effect Effects 0.000 description 37
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 17
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- FZHAPNGMFPVSLP-UHFFFAOYSA-N silanamine Chemical compound [SiH3]N FZHAPNGMFPVSLP-UHFFFAOYSA-N 0.000 description 14
- 239000000872 buffer Substances 0.000 description 11
- 239000000047 product Substances 0.000 description 11
- 239000012064 sodium phosphate buffer Substances 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 9
- 238000003795 desorption Methods 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 230000003321 amplification Effects 0.000 description 8
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- 238000006243 chemical reaction Methods 0.000 description 8
- 239000000412 dendrimer Substances 0.000 description 8
- 229920000736 dendritic polymer Polymers 0.000 description 8
- 238000003199 nucleic acid amplification method Methods 0.000 description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
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- 238000001179 sorption measurement Methods 0.000 description 7
- 239000000232 Lipid Bilayer Substances 0.000 description 6
- 229910021642 ultra pure water Inorganic materials 0.000 description 6
- 239000012498 ultrapure water Substances 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- BLSAPDZWVFWUTL-UHFFFAOYSA-N 2,5-dioxopyrrolidine-3-sulfonic acid Chemical compound OS(=O)(=O)C1CC(=O)NC1=O BLSAPDZWVFWUTL-UHFFFAOYSA-N 0.000 description 5
- 239000010419 fine particle Substances 0.000 description 5
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 5
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 239000006249 magnetic particle Substances 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- ASNTZYQMIUCEBV-UHFFFAOYSA-N 2,5-dioxo-1-[6-[3-(pyridin-2-yldisulfanyl)propanoylamino]hexanoyloxy]pyrrolidine-3-sulfonic acid Chemical compound O=C1C(S(=O)(=O)O)CC(=O)N1OC(=O)CCCCCNC(=O)CCSSC1=CC=CC=N1 ASNTZYQMIUCEBV-UHFFFAOYSA-N 0.000 description 4
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- 238000012408 PCR amplification Methods 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000002411 adverse Effects 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
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- 230000009881 electrostatic interaction Effects 0.000 description 3
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- 239000011859 microparticle Substances 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- 229940080818 propionamide Drugs 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- -1 succinimidyl group Chemical group 0.000 description 3
- 238000004381 surface treatment Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- HXMVNCMPQGPRLN-UHFFFAOYSA-N 2-hydroxyputrescine Chemical compound NCCC(O)CN HXMVNCMPQGPRLN-UHFFFAOYSA-N 0.000 description 2
- 101150038502 ALDH2 gene Proteins 0.000 description 2
- 102000005369 Aldehyde Dehydrogenase Human genes 0.000 description 2
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000003196 chaotropic effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 150000002430 hydrocarbons Chemical class 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- WHMDPDGBKYUEMW-UHFFFAOYSA-N pyridine-2-thiol Chemical compound SC1=CC=CC=N1 WHMDPDGBKYUEMW-UHFFFAOYSA-N 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 238000013382 DNA quantification Methods 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000006087 Silane Coupling Agent Substances 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- UTKFUXQDBUMJSX-UHFFFAOYSA-N boron neodymium Chemical compound [B].[Nd] UTKFUXQDBUMJSX-UHFFFAOYSA-N 0.000 description 1
- RDHPKYGYEGBMSE-UHFFFAOYSA-N bromoethane Chemical compound CCBr RDHPKYGYEGBMSE-UHFFFAOYSA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 150000004662 dithiols Chemical class 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000007614 genetic variation Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000005381 magnetic domain Effects 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000001921 nucleic acid quantification Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 150000003613 toluenes Chemical class 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005104816A JP2006280277A (ja) | 2005-03-31 | 2005-03-31 | 核酸の抽出方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005104816A JP2006280277A (ja) | 2005-03-31 | 2005-03-31 | 核酸の抽出方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006280277A true JP2006280277A (ja) | 2006-10-19 |
| JP2006280277A5 JP2006280277A5 (enExample) | 2008-04-24 |
Family
ID=37402823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005104816A Pending JP2006280277A (ja) | 2005-03-31 | 2005-03-31 | 核酸の抽出方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2006280277A (enExample) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010110435A1 (ja) * | 2009-03-27 | 2010-09-30 | 国立大学法人岡山大学 | 有機・無機複合材料及びその製造方法 |
| JP2011057813A (ja) * | 2009-09-09 | 2011-03-24 | Tokyo Univ Of Agriculture & Technology | デンドリマー被覆磁気微粒子並びにその製造方法及び用途 |
| JP2013505719A (ja) * | 2009-09-24 | 2013-02-21 | キアジェン ゲイサーズバーグ インコーポレイテッド | 陰イオン交換材料を使用した核酸の単離および分析のための組成物、方法およびキット |
| US8795546B2 (en) | 2009-12-15 | 2014-08-05 | National University Corporation Okayama University | Magnetic ceramic and process for production thereof |
| CN115041143A (zh) * | 2022-04-02 | 2022-09-13 | 中国医学科学院基础医学研究所 | 一种磁性聚合物、制备方法、试剂盒及应用 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002016528A1 (en) * | 2000-08-21 | 2002-02-28 | National Institute Of Advanced Industrial Science And Technology | Magnetic particles and process for producing the same |
| JP2004150797A (ja) * | 2002-09-17 | 2004-05-27 | Yokogawa Electric Corp | デンドリマーによる核酸またはタンパク質の抽出方法およびデンドリマー組成物 |
| JP2004329072A (ja) * | 2003-05-02 | 2004-11-25 | Canon Inc | 磁性体−生体物質複合体型構造体、磁性体に対して結合能を有するアミノ酸配列を有するペプチド断片及びその遺伝子、ならびに磁性体−生体物質複合体型構造体の製造方法 |
| JP2005017013A (ja) * | 2003-06-24 | 2005-01-20 | Hitachi Maxell Ltd | 生体物質結合用磁性担体 |
| WO2005012522A1 (en) * | 2003-07-23 | 2005-02-10 | Cyclops Genome Sciences Limited | Clean-up beads |
-
2005
- 2005-03-31 JP JP2005104816A patent/JP2006280277A/ja active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002016528A1 (en) * | 2000-08-21 | 2002-02-28 | National Institute Of Advanced Industrial Science And Technology | Magnetic particles and process for producing the same |
| JP2004150797A (ja) * | 2002-09-17 | 2004-05-27 | Yokogawa Electric Corp | デンドリマーによる核酸またはタンパク質の抽出方法およびデンドリマー組成物 |
| JP2004329072A (ja) * | 2003-05-02 | 2004-11-25 | Canon Inc | 磁性体−生体物質複合体型構造体、磁性体に対して結合能を有するアミノ酸配列を有するペプチド断片及びその遺伝子、ならびに磁性体−生体物質複合体型構造体の製造方法 |
| JP2005017013A (ja) * | 2003-06-24 | 2005-01-20 | Hitachi Maxell Ltd | 生体物質結合用磁性担体 |
| WO2005012522A1 (en) * | 2003-07-23 | 2005-02-10 | Cyclops Genome Sciences Limited | Clean-up beads |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010110435A1 (ja) * | 2009-03-27 | 2010-09-30 | 国立大学法人岡山大学 | 有機・無機複合材料及びその製造方法 |
| US20120034670A1 (en) * | 2009-03-27 | 2012-02-09 | Takashi Sakai | Organic-inorganic composite material and process for producing same |
| US8841105B2 (en) * | 2009-03-27 | 2014-09-23 | National University Corporation Okayama University | Organic-inorganic composite material and process for producing same |
| JP5622718B2 (ja) * | 2009-03-27 | 2014-11-12 | 国立大学法人岡山大学 | 有機・無機複合材料及びその製造方法 |
| JP2011057813A (ja) * | 2009-09-09 | 2011-03-24 | Tokyo Univ Of Agriculture & Technology | デンドリマー被覆磁気微粒子並びにその製造方法及び用途 |
| JP2013505719A (ja) * | 2009-09-24 | 2013-02-21 | キアジェン ゲイサーズバーグ インコーポレイテッド | 陰イオン交換材料を使用した核酸の単離および分析のための組成物、方法およびキット |
| US8795546B2 (en) | 2009-12-15 | 2014-08-05 | National University Corporation Okayama University | Magnetic ceramic and process for production thereof |
| CN115041143A (zh) * | 2022-04-02 | 2022-09-13 | 中国医学科学院基础医学研究所 | 一种磁性聚合物、制备方法、试剂盒及应用 |
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