JP2006217916A - (リグノ)セルロース材料の酵素による加水分解物のエタノール発酵からの蒸留残渣を用いてセルロース分解性およびヘミセルロース分解性の酵素を生産する方法 - Google Patents
(リグノ)セルロース材料の酵素による加水分解物のエタノール発酵からの蒸留残渣を用いてセルロース分解性およびヘミセルロース分解性の酵素を生産する方法 Download PDFInfo
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Abstract
【解決手段】本方法は、(リグノ)セルロース性の材料の酵素加水分解物のエタノール発酵からの残渣を用いる。本方法は、(リグノ)セルロース性の材料からエタノールを生産する方法であって、(リグノ)セルロース性の材料を化学的および/または物理的に前処理する工程と、セルロース分解性および/またはヘミセルロース分解性の酵素を用いて前処理された材料を酵素加水分解する工程と、適切なアルコール生成性の微生物によって前記加水分解物をエタノール発酵させ、発酵マストを生産する工程と、前記アルコール生成性の微生物を分離し、エタノールを分離/精製し、残渣を構成する水相を生産する工程とを包含し、該残渣は、工程2)のセルロース分解性および/またはヘミセルロース分解性の酵素の生産のために機能する、方法に統合されてもよい。
【選択図】図2
Description
セルロース分解性酵素の生産のための工業的な方法において、ラクトースは、相変わらず、最も適切な基質の一つおよび最も安価なものの一つであるが、しかしながら、依然として高価であり、酵素の費用価格の約3分の1を示す。なされた進歩全てにも拘わらず、セルロース性のバイオマスをエタノールに変換する場合の酵素の費用は相変わらず高く、30〜50%であり、さらに、セルラーゼの生産のための炭素源としてラクトースを用いる場合に、この方法は外的な炭素源に依存する。この理由のために、紡績糸(spinning)、例えば加水分解されたヘミ−セルロースからの炭素含有基質の使用は、誘導炭素源が容易に入手可能であれば大きな前進である。
セルラーゼの生産は、機械的に攪拌された発酵槽において行われた。培地は、以下の組成を有する:KOH,1.66g/l; 85%H3PO4,2ml/l, (NH4)2SO4,2.8g/l; MgSO4・7H2O,0.6g/l; CaCl2,0.6g/l; MnSO4,3.2mg/l; ZnSO4・7H2O,2.8mg/l; CoCl2,4.0mg/l; FeSO4・7H2O,10mg/l; コーン・スティープ,1.2g/l; 消泡剤,0.5ml/l。
酵素の生産は、実施例1と同一の条件下に行われた。
この実施例では、誘導基質としてラクトースが用いられ、初期のキシロース排出後の酵素生産期に単独で注入された。
実施例2に記載された条件下に発酵が行われた。バイオマスは、20g/lのキシロースにより生産されたバッチであった。キシロースが加えられた残渣から調製された溶液を有する供給バッチにおいて酵素が生産された。参照符号H1を有する残渣の溶液が次のようにして調製された。
実施例4に記載されたように、バイオマスは、20g/lのキシロースにより生産されたバッチであった。キシロースが加えられた残液から調製された基質の溶液を有する供給バッチにおいて酵素が生産された。本実施例の場合、参照符号H2を有する残液の溶液は、H1について記載されたように調製されたが、加水分解は、24時間の加水分解後の初期添加と同一の酵素を加えながら48時間まで延長された。加水分解物は、110.8g/lのグルコース、11.8g/lのキシロースを含んでいた。エタノールを除去し濃縮した後の残液H2が、全糖 200g/lを含む1.5リットルの基質溶液を調製するために用いられた。実施例4の条件下に発酵が行われた。発酵は、158時間後に停止された。
Claims (9)
- セルロース分解性の微生物によってセルロース分解性および/またはヘミセルロース分解性の酵素を生産する方法であって、
セルロース性またはリグノ−セルロース性材料の酵素加水分解物のエタノール発酵からの残渣を用いることを特徴とする方法。 - 前記残渣は、セルロース分解性微生物の生育およびセルロース分解性および/またはヘミセルロース分解性の酵素の生産のための炭素源として用いられる、請求項1に記載の方法。
- 前記残渣は、セルロース分解性微生物の生育のために用いられる他の炭素源への補給としての誘導炭素源として用いられる、請求項1または2に記載の方法。
- 前記残渣は、セルロース分解性の微生物の生育およびセルロース分解性および/またはヘミセルロース分解性の酵素の生産のための唯一の炭素源として用いられる、請求項1または2に記載の方法。
- セルロース分解性の微生物は、トリコデルマ、アスペルギルス、ペニシリウムおよびスエヒロタケ属からの菌類から選択される、請求項1〜4のいずれか1つに記載の方法。
- セルロース分解性の微生物は、トリコデルマ・リーゼイ種に属する、請求項5に記載の方法。
- セルロース性またはリグノ−セルロース性の材料は、わら、木材、森林栽培物、アルコール生成性植物、精糖所および穀類加工業者からの残渣、製紙産業からの残渣およびセルロース性またはリグノ−セルロース性材料からの変換産物から選択される、請求項1〜6のいずれか1つに記載の方法。
- セルロース性またはリグノ−セルロース性の材料からエタノールを生産する方法であって、
1)セルロース性またはリグノセルロース性の材料を化学的および/または物理的に前処理する工程と、
2)セルロース分解性および/またはヘミセルロース分解性の酵素を用いて、前処理された材料を酵素加水分解する工程と、
3)適切なアルコール生成性の微生物によって、工程2)からの加水分解物をエタノール発酵させ、発酵マストを生産する工程と、
4)工程3)において用いられたアルコール生成性の微生物を分離し、エタノールを分離/精製し、残渣を構成する水相を得る工程と
を包含する方法において、
該方法は、工程2)において用いられたセルロース分解性および/またはヘミセルロース分解性の酵素を請求項1〜7のいずれか1つに記載の方法によって生産するために、該残渣が用いられることを特徴とする方法。 - 工程4)において、蒸留によってエタノールが発酵マストから分離され、残渣は蒸留残液によって構成される請求項8に記載の方法。
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FR0501371A FR2881753B1 (fr) | 2005-02-09 | 2005-02-09 | Procede de production d'enzymes cellulolytiques et hemicellulolytiques utilisant les residus de distillation de fermentation ethanolique d'hydrolysats enzymatiques de materiaux (ligno-)cellulosique |
FR0501371 | 2005-02-09 |
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Cited By (4)
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WO2008090707A1 (ja) * | 2007-01-25 | 2008-07-31 | Japan International Research Center For Agricultural Sciences | エタノール又は乳酸の製造方法 |
WO2009008206A1 (ja) * | 2007-07-10 | 2009-01-15 | Shigadry With Earth Co., Ltd. | エタノールの製造方法 |
JP2010017084A (ja) * | 2008-07-08 | 2010-01-28 | Oji Paper Co Ltd | 糖化発酵システム |
JP2014521359A (ja) * | 2011-08-19 | 2014-08-28 | イエフペ エネルジ ヌヴェル | 低い酸素移動容量係数KLaを有する、発酵槽に適した糸状菌を用いるセルラーゼの生産方法 |
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EP1690944B1 (fr) | 2011-11-02 |
FR2881753A1 (fr) | 2006-08-11 |
ES2376636T3 (es) | 2012-03-15 |
FR2881753B1 (fr) | 2009-10-02 |
EP1690944A1 (fr) | 2006-08-16 |
CA2535246A1 (fr) | 2006-08-09 |
NO335190B1 (no) | 2014-10-20 |
US7494792B2 (en) | 2009-02-24 |
CA2535246C (fr) | 2014-04-15 |
ATE531810T1 (de) | 2011-11-15 |
DK1690944T3 (da) | 2012-02-27 |
BRPI0600409B1 (pt) | 2017-05-30 |
PL1690944T3 (pl) | 2012-04-30 |
JP5498642B2 (ja) | 2014-05-21 |
BRPI0600409A (pt) | 2006-10-03 |
NO20060599L (no) | 2006-08-10 |
US20060177917A1 (en) | 2006-08-10 |
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