JP2006169149A - Epidermal basal cell-growth promoter of skin - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain an epidermal basal cell-growth promoter of skin containing an extract of Garcinia mangostana Linne. <P>SOLUTION: The epidermal basal cell-growth promoter of skin contains an extract of Garcinia mangostana Linne. as an active ingredient obtained by immersing fruit of Garcinia mangostana Linne. in water and an organic solvent. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a skin epidermal basal cell proliferation promoter containing a mangosteen extract.

Skin aging causes various symptoms such as sagging, spots and wrinkles in the skin, and is significantly accelerated by ultraviolet rays. The main cause is considered to be active oxygen produced by ultraviolet rays (cosmetic science, 32 pages, published by Asakura Shoten). For this reason, many applications have been filed regarding ultraviolet absorbers and antioxidants. Recently, matrix metalloprotease inhibitors have been disclosed in JP-A-2003-252745 as one method for preventing aging.

However, all of these suppress aging, and none of the skin epidermal basal cells are activated to maintain the youthfulness of the skin by promoting cell proliferation.
JP 2003-252745 A

  This invention is made | formed in view of the said problem, and it aims at providing the skin epidermal basal cell proliferation promoter containing a mangosteen extract.

  As a result of screening the skin epidermal basal cell proliferation promoting effect on various plant extracts for the purpose of obtaining a useful skin epidermal basal cell proliferation promoting agent, the present inventors have found that the mangosteen extract has a skin epidermal basal cell proliferation promoting action. As a result, the present invention has been completed. That is, the present invention relates to a skin epidermal basal cell proliferation promoter comprising a mangosteen extract as an active ingredient.

  According to the present invention, the skin epidermal basal cell proliferation promoter comprising a mangosteen extract produced from edible mangosteen fruit as an active ingredient is safe and has a proliferation promoting effect on skin epidermal basal cells. , It can be used for skin external preparation and bath preparation. That is, by maintaining the youthfulness of the skin, it is expected to maintain elastic skin without spots, wrinkles or sagging, skin diseases such as eczema, atopic dermatitis and rough skin, and promote healing of wounds. .

Mangosteen said by the present invention (scientific name: Garcinia mangostana L.) is a hypericaceae plant originating in Thailand and is widely cultivated in the Southeast Asian region. The pericarp is used as a treatment for cholera and dysentery, to prevent diarrhea, and as a central nervous system sedative and blood pressure increasing agent. The fruit to be used may be either immature or fully ripe, meaning the whole fruit and the fruit skin, which may be dried or undried (raw), and is used after being crushed with a crusher or shredded with a slicer.

  The solvent used for extraction in the present invention is water and an organic solvent. Examples of the organic solvent include alcohols such as methanol, ethanol, propanol and butanol, hydrocarbons such as hexane and benzene, ethers such as diethyl ether, acetic acid. Esters such as methyl and ethyl acetate, ketones such as acetone, halogenated hydrocarbons such as methylene chloride and chloroform, organic acids that are liquid at room temperature such as acetic acid, glacial acetic acid and propionic acid, etc., single or mixed And use. The usage-amount is 1-50 parts with respect to 1 part of mangosteen fruit. If it is 1 part or less, the yield of the extract is low, and if it is 50 parts or more, it takes time to distill off the solvent, which is not preferable.

The mangosteen extract of the present invention can be produced as follows.
1 part (weight) of immature mangosteen fruit is sliced or crushed and heated in 1 to 50 parts of water. Floating yellow substance is collected by a conventional method and dried with a normal dryer.
Sliced or crushed 1 part (by weight) of unripe mangosteen fruit or ripe mangosteen fruit or fruit skin, immersed in water-containing organic solvent or 1 to 50 parts of organic solvent at room temperature to boiling temperature for several hours to several days, sliced or Remove the crushed fruit or peel. The filtrate is collected after concentration at normal pressure or reduced pressure by a conventional method, or the solvent is distilled off and dried in a normal dryer.

The extract can be purified by silica gel and reverse phase column chromatography.
The heating treatment referred to in the present invention is to bring the immersion liquid to a temperature of room temperature or higher, and preferably means an operation (step) of immersion at a temperature higher than 40 ° C.

  The skin epidermal basal cell proliferation promoter containing the mangosteen extract of the present invention as an active ingredient is used alone or in combination with ingredients or additives usually used in cosmetics, topical skin preparations and bath preparations. It can be used for the manufacture of external preparations and bath preparations. The blending amount is 0.0001 to 50% by mass (hereinafter, “%” means “% by mass”), preferably 0.001 to 40%. If 0.0001% or less, sufficient effect is obtained. I can't.

  Examples of ingredients and additives that are commonly used in cosmetics, external skin preparations, and bath preparations include moisturizers, antioxidants, whitening agents, UV absorbers, anti-inflammatory agents, bactericides, antiallergic agents, and blood flow improvement. Agent, surfactant, thickener, preservative, pH adjuster, refresher, stabilizer, emulsifier, solvent, chelating agent, colorant, fragrance, inorganic pigment, alcohol, fats and oils, waxes, fatty acids , Esters, polysaccharides, vitamins, hormones, enzymes, amino acids, peptides, water, aqueous components, powder components, and the like.

  Examples of cosmetics and skin external preparations using the skin epidermal basal cell proliferation promoter of the present invention include skin lotion, emulsion, cream, ointment, lotion, oil, pack, soap, shampoo, hair treatment, foundation, funnier, skin Examples include spraying drugs.

Hereinafter, the present invention will be described in detail with reference to examples and test examples.
Example 1
After 835 g of immature mangosteen fruit was sliced, it was immersed in 4.6 kg of water at 50 ° C. for 1 hour. A yellowish brown substance floating in the light brown liquid was collected with a filter cloth and dried at 65 ° C. to obtain 9.9 g of a mangosteen extract as a fine yellowish brown powder.

Example 2
173 g of ripened mangosteen peel was added to 500 g of 65% ethanol and immersed at 60 ° C. for 5 hours. After cooling, it was filtered through filter paper, and the filtrate was concentrated under reduced pressure to distill off ethanol. The concentrate was centrifuged to collect a pale yellow precipitate and dried under reduced pressure at 40 ° C. to obtain 3.1 g of a mangosteen extract as a pale yellow fine powder.

Example 3
After 3 kg of dried cut pieces of immature mangosteen fruit were soaked in 30 kg of 60% ethanol at 60 ° C. overnight, they were filtered with a filter press, and the filtrate was concentrated under reduced pressure to distill off the ethanol. The concentrate was lyophilized to obtain 810 g of a mangosteen extract as a light tan powder.

Test example 1
10-50 epidermal basal cells according to the method described in pages 194-5 of Modern Dermatology University Vol. 4 B Test II Experiment Method (published by Nakayama Shoten) Small cell masses were prepared. 2 ml of Eagle MEM in which this small cell mass is suspended is poured into a sterile petri dish with a diameter of 60 mm, cultured in a carbon dioxide incubator at 30 ° C. for 24 hours, and then a DMSO solution of the mangosteen extract obtained in Example 1 at each concentration. 1 ml of Eagle's MEM to which was added was added and further cultured for 9 days. The total area of the cell sheet generated in each petri dish was measured with an image analyzer. As a result, the total area of the mangosteen extract addition group when the total area of the control (addition concentration: 0%) is 100 is 115 when the addition concentration in the medium is 0.0025% and 129 when the addition concentration is 0.005%. , 0.01% for 157, 0.015% for 177, 0.02% for 186. As the concentration of mangosteen extract increased, the total area increased and the proliferation of epidermal basal cells was observed.

Test example 2
Appropriate amount of the mixture obtained by thoroughly mixing 40 g of the mangosteen extract obtained in Example 3, 30 g of polyethylene glycol 1000 and 30 g of kaolin was once a day on the affected area where the elbow joint was pigmented and itchy. For 7 days. As shown in FIG. 1, the itch of the affected area where pigmentation has progressed before application disappears as a result of application, and at the same time the proliferation of skin epidermal basal cells is promoted, and the exfoliation of the pigmented stratum corneum begins gradually. Seven days later, it completely returned to its original condition and was completely cured.

Figure showing changes in the affected skin before and after application

Claims (1)

A skin epidermal basal cell proliferation promoter comprising a mangosteen extract.