JPWO2008018133A1 - Moisturizer, cell activator, whitening agent, neutral fat accumulation inhibitor, antioxidant, and external preparation for skin - Google Patents

Abstract

The present invention relates to a moisturizer, a cell activator, a whitening agent, a neutral fat accumulation inhibitor, and an antioxidant containing one or more plants selected from Osmundaceae plants or extracts thereof as active ingredients. It relates to the agent. Furthermore, the skin external preparation containing the 1 type, 2 or more types of plant chosen from the Osmundaceae plant, or its extract is disclosed.

Description

The present invention relates to a moisturizer, a cell activator, a whitening agent, a neutral fat accumulation inhibitor, an antioxidant, and an external preparation for skin, each of which contains a naturally derived component as an active ingredient. More specifically, the present invention relates to a moisturizer, a cell activator, a whitening agent, a neutral fat accumulation inhibitor, an antioxidant, and an external preparation for skin, which contains a plant extract of Osmundaceae .

  Causes of skin symptoms such as aging, disease, stress, wrinkles due to ultraviolet rays, blemishes, reduced skin elasticity, dryness, decreased cellular function, melanin production and pigmentation due to ultraviolet rays, reduction and degeneration of dermal matrix components, ultraviolet rays, etc. Oxidative damage of cells due to. In order to prevent and ameliorate such skin symptoms, various active ingredients have been searched and formulated. In particular, naturally-derived components are known to have various pharmacological and cosmetic effects, and so far, applications of extracts such as plants and fungi to skin external preparations have been studied.

  For example, orchids (see JP 2002-205933), agave extracts (see JP 2002-193820), peanut seed coat extracts (see JP 2002-145757), psyllium as moisturizers Extracts (see JP 2002-145756 A), extract of longan seeds (see JP 2002-145732 A), and psyllium extracts (see JP 2002-145731 A) are known. The essence of Ponkan as a cell activator (see JP 2001-131045), the water and / or organic solvent extract of Hakutsuru Reishi as a whitening agent (see JP 2003-89630), a neutral fat accumulation inhibitor As an antioxidant, an extract of a plant belonging to the genus Sarphagaceae (see Japanese Patent Application Laid-Open No. 10-182413) is known as an antioxidant.

  Thus, various naturally-derived components have been applied so far. However, there are many naturally-derived ingredients whose effects are not yet known, and they have an excellent moisturizing action, cell activation action, whitening action, neutral fat accumulation inhibiting action, antioxidant action, etc. The development of ingredients was expected.

  As a result of examining various components derived from nature, the present inventors have found that the effect of the genus genus plant, which has not been known for its effects, is superior in moisturizing action, cell activation action, whitening action, and neutral fat accumulation. The present inventors have found that an inhibitory action and an antioxidant action exist, and have further studied to complete the present invention.

That is, the present invention relates to a moisturizer, cell activator, whitening agent, neutral fat accumulation inhibitor, or antioxidant comprising one or more plants selected from the spring family plants or an extract thereof as an active ingredient. About.
Another aspect of the present invention relates to a skin external preparation characterized by containing one or more plants selected from the genus genus plants or extracts thereof.

According to the present invention, a moisturizer, a cell activator, a whitening agent, a neutral fat having an excellent effect by using one or two or more kinds of plants selected from the spring plant or an extract thereof as an active ingredient. Accumulation inhibitors and antioxidants can be provided.
By blending these moisturizers, cell activators, whitening agents, neutral fat accumulation inhibitors, and antioxidants into skin external preparations such as cosmetics and external medicines, wrinkles, tarmi, reduced skin elasticity, Various compositions that exhibit excellent effects in preventing and improving the appearance of various skin symptoms such as dullness can be provided.

Spring family (Osmundaceae) plant is a primitive fern plant, osmunda (Osmunda), Todea genus (Todea), such as streptomycin Puterisu genus (Leptopteris) are known. The osmunda (Osmunda) plants mainspring (Osmunda japonica), Regal mainspring (Osmunda regalis), Yasha mainspring (Osmunda lancea), Obayashi catcher mainspring (Osmunda intermedia), Copper Pheasant mainspring (Osmunda cinnamomea), Onizenmai (Osmunda claytoniana), Shiroyama spring ( Osmunda banksifolia ) is known.
These plants can be used alone or in combination of two or more.

In the present invention, it is not particularly limited as long as it is a member of the genus genus, but an example is an oyster ( Osmunda japonica ) because of its relatively easy availability.

When using these nymphae plants, there are no particular limitations on the site of use, and any site such as the root, stem, stem, leaf, or young bud of a spore or gametophyte can be used. A plurality of parts may be used in combination.
They can be pulverized as they are, but it is preferable to use an extract from those parts.

For extraction, any part of the sporophyte or gametophyte of the spring family plant may be used, but for easy use, the whole plant, root, leaf blade, petiole, etc. of the spore body may be used. In that case, you may make it obtain an extract using a some site | part. Further, two or more kinds of extracts extracted using different solvents may be mixed and used.
In the extraction, the plant may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization.
The extraction can be performed by immersing in an arbitrary extraction solvent for a predetermined time. The extraction solvent may be heated as necessary. Alternatively, extraction can be performed using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, stirring or homogenization in an extraction solvent may be performed. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 1 hour to 14 days.

  As an extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether And solvents such as esters such as butyl acetate and ethyl acetate; ketones such as acetone and ethyl methyl ketone. These may be used alone or in combination of any two or more. Saline, phosphate buffer, phosphate buffered saline, and the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical liquids and subcritical liquids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  The extract of the oyster family plant can be used as it is, but it can be used by aging it for a certain period of time, or the concentrated and dried product can be dissolved again in water or a polar solvent. Can also be used. Alternatively, it may be used after performing purification treatment such as decolorization, deodorization, desalting, etc., or fractionation treatment by column chromatography or the like, as long as these physiological functions are not impaired. The above-mentioned extract of the genus genus plant and its treated product and fractionated product can be freeze-dried after each treatment and fractionation and dissolved in a solvent at the time of use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

Windmill plant or its extract has excellent moisturizing action, cell activation action, whitening action, neutral fat accumulation inhibitory action, antioxidant action, moisturizer, cell activator, whitening agent, neutral fat accumulation inhibitory It can be used as an agent and an antioxidant.
Each of these agents is not limited at all in terms of its form and the presence or absence of other components, as long as it includes a genus plant or an extract thereof as an active ingredient. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected according to its use and the like, vehicle (excipient), solvent, Other general additives (antioxidants, colorants, dispersants, etc.) can optionally be included.

  A moisturizing agent containing a spring family plant or its extract as an active ingredient provides an excellent moisturizing effect on the skin and hair, etc., and also exhibits an excellent effect on improving skin symptoms such as rough skin, fine lines, dullness, and the like. To improve skin transparency.

  A cell activator comprising a nymphae plant or an extract thereof as an active ingredient has an excellent dermal fibroblast activation effect and an epidermal cell activation effect, and exhibits an excellent cell activation effect.

  A whitening agent comprising a genus plant or its extract as an active ingredient has an excellent melanin production inhibitory effect and tyrosinase activity inhibitory effect, and prevents and improves pigmentation, stains, freckles, etc., and has an excellent whitening effect Demonstrate.

  A neutral fat accumulation inhibitor containing an apricot family plant or an extract thereof as an active ingredient has an excellent neutral fat accumulation inhibitory effect and exhibits an excellent neutral fat accumulation inhibitory effect.

  Antioxidants containing the oyster plant or its extract as an active ingredient have an excellent radical scavenging effect and a superoxide anion scavenging effect and exhibit an excellent antioxidant action.

  Prevents / improves various skin symptoms such as wrinkles, tarmi, stains, dullness, dryness, fine wrinkles, etc. by blending spring plants or their extracts into skin preparations such as cosmetics, topical medicines, and quasi-drugs An external preparation for skin that exhibits an excellent effect can be obtained. Therefore, for example, it can be preferably used as a skin external preparation for moisturizing or a skin external preparation for whitening.

  The amount of the spring plant or its extract in the external preparation for skin can be adjusted depending on the type and purpose of the external preparation for skin, but from the standpoint of effects and stability, In terms of solid content, 0.0001 to 10.0% by mass is preferable, more preferably 0.001 to 5.0% by mass, still more preferably 0.01 to 5% by mass, and still more preferably 0. .1 to 5% by mass.

  The dosage form of the external preparation for skin is arbitrary. For example, it should be provided in various dosage forms such as lotion, emulsion, gel, cream, ointment, powder, granule, pack, patch, patch, aerosol, etc. Can do.

  As an external preparation for skin, in addition to a genus plant or an extract thereof, an optional agent that is usually blended in pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics and cleansing agents, etc. Components such as water, oily components, moisturizers, powders, pigments, emulsifiers, solubilizers, gelling agents, cleaning agents, UV absorbers, anti-inflammatory agents, thickeners, pH adjusters, surfactants, Chelating agents, drugs (medicinal ingredients), fragrances, resins, antibacterial and antifungal agents, antioxidants, alcohols and the like can be appropriately blended. Furthermore, within the range not impairing the effects of the present invention, other moisturizers, cell activators, whitening agents, neutral fat accumulation inhibitors, antioxidants, or combinations with plants other than the genus plant or extracts thereof Is possible.

  In the following, preparation examples of spring plant extracts, tests for evaluating each action, formulation examples as a skin external preparation, and use tests will be described in more detail, but the technical scope of the present invention is not limited thereby. It is not something.

<Preparation method 1>
20 liters of a 50% by weight aqueous ethanol solution was added to 1 kg of a dry pulverized whole plant spores of Osmunda japonica and soaked at room temperature for 7 days. The extract was collected by filtration and the solvent was removed, and then 30 g of a spring family plant extract was obtained.

<Preparation method 2>
20 liters of water was added to 1 kg of a dry pulverized whole body of a sprout of a mainspring and extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration and the solvent was removed, and then 20 g of a spring family plant extract was obtained.

<Preparation method 3>
20 kg of methanol was added to 1 kg of a dry pulverized whole body of a sprout of a mainspring and immersed at room temperature for 7 days. The extract was collected by filtration and the solvent was removed, and then 50 g of a genus plant extract was obtained.

<Preparation method 4>
100 g of a dry pulverized whole body of a sprout of a mainspring was put into a supercritical extraction apparatus, and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under an atmospheric pressure of 15 Mpa. The extract was collected to obtain 10 g of the spring family plant extract.

<Evaluation of cell activation action 1 (dermis fibroblast activation action)>
Evaluation of the dermis fibroblast activation action of the springfish plant extract was performed by the following method. As a sample, a genus plant extract produced by Preparation Method 1 was used.
Normal human dermal fibroblasts manufactured by Kurabo Industries Co., Ltd. (Kurashiki Boseki Co., Ltd.) were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) to which 1% by mass of fetal bovine serum (FBS) was added was used. After culturing for 24 hours, the medium was replaced with 1% by mass FBS-added DMEM medium to which a sample (extract) was added so as to have each concentration shown in Table 1, and further cultured for 48 hours. After removing the supernatant, the medium was replaced with a medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) and cultured for about 2 hours. Thereafter, formazan produced by the opening of the tetrazolium ring was extracted with 2-propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. In the evaluation, in addition to the medium containing the sample, a 1% by mass FBS-added DMEM medium was used as a negative control, and a 5% by mass FBS-added DMEM medium was used as a positive control.
The evaluation results are shown in Table 1 as relative values with the cell activation effect in the negative control as 100.

  As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with the genus plant extract. From this, it was clarified that the spring-like plant extract has an excellent dermal fibroblast activation effect.

<Evaluation 2 of cell activation action (epidermal cell activation action)>
Evaluation of the epidermis cell activation effect of the springfish plant extract was performed by the method shown below. As a sample, a genus plant extract produced by Preparation Method 2 was used.
Human epidermal non-keratinized cells were seeded in a 96-well microplate so that there were 2.0 × 10 ⁴ cells per well. As a seeding medium, commercially available Humdia-KG2 manufactured by Kurabo Industries Co., Ltd. was used. After culturing for 24 hours, the culture medium was replaced with the test medium added with the sample at the concentration shown in Table 2, and further cultured for 24 hours. Subsequently, the medium is exchanged with a medium containing 100 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT), cultured for 2 hours, and then generated by ring opening of the tetrazolium ring. Formazan was extracted with 2-propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values.
The evaluation results are shown in Table 2 as relative values when the cell activation effect in the control using the medium without the sample is taken as 100.

As is clear from Table 2, a significant epidermal cell activation effect was observed in the medium supplemented with the genus plant extract. From this, it was found that the spring plant extract has an excellent epidermal cell activation effect.
From the above, it has been clarified that the springfish plant extract has an excellent cell activation effect.

<Evaluation of whitening action 1 (melanin production inhibitory action)>
Evaluation of the melanin production inhibitory action of the springfish plant extract was performed by the method shown below. As a sample, a genus plant extract produced by Preparation Method 3 was used.
B16 mouse melanoma cells (B16F0 cells) were seeded at 1.8 × 10 ⁴ per dish. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) to which 5% by mass fetal bovine serum (FBS) was added was used. After 24 hours, the sample-added medium adjusted to each concentration shown in Table 4 was replaced with 5% by mass FBS-added DMEM medium, and further cultured for 5 days. After completion of the culture, the cells were detached with trypsin, transferred to a 1.5 mL microtube and centrifuged to obtain a cell precipitate. The color of the obtained precipitate was visually determined based on the determination table shown in Table 3. Evaluation was based on a 5-stage evaluation, and 5% by mass FBS-added DMEM medium containing no sample was used for negative control (determination 5), and 5% by mass FBS-added DMEM medium containing 50 mM sodium lactate was used for positive control (determination 1).
Moreover, in order to evaluate the amount of melanin produced, Solen-350 (Perkin Elmer Japan Co., Ltd.) was added to the precipitate and boiled, and 500 nm was measured with a spectrophotometer (Spectrophotometer U-3010 manufactured by Hitachi High-Technologies Corporation). The absorbance at was measured.
Table 4 shows the obtained results.

  As is clear from Table 4, a significant melanin production-suppressing effect was observed in the medium added with the oyster family plant extract. From this, it was found that the spring-like plant extract has an excellent melanin production inhibitory action.

<Evaluation of whitening action 2 (tyrosinase activity inhibitory action)>
Evaluation of the tyrosinase activity inhibitory action of the springfish plant extract was carried out by the following method. As a sample, a genus plant extract produced by Preparation Method 1 was used.
Normal human epidermal melanocytes manufactured by Kurabo Industries Co., Ltd. were seeded in a 96-well microplate so that the number of cells was 3.0 × 10 ⁴ per well. As a seeding medium, Medium154S manufactured by Kurabo Industries Co., Ltd. was used. After 24 hours, the medium was replaced with a sample-added medium adjusted to each concentration shown in Table 5 with Medium 154S, and further cultured for 48 hours. Next, the cells were completely lysed by exchanging with 75 μL of 1 mass% Triton-X-containing phosphate buffer, and 50 μL of the cells was used as a crude enzyme solution. To the crude enzyme solution, 50 μL of 0.05% by mass L-dopa-containing phosphate buffer serving as a substrate was added and allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm was measured immediately after the addition of the substrate and at the end of the reaction with a microplate reader, and the amount of produced dopamelanin was determined by introducing each measured value into Equation (1).
Formula (1): Amount of produced dopamelanin = {(405 nm value after reaction−405 nm value before reaction)} − 2.166 / 5.238
Moreover, the protein amount of each well was measured by BCA Protein Assay Kit made by PIERCE, and the amount of dopamelanin produced per unit protein amount was determined. The measurement results are shown in Table 5 according to the inhibition rate with reference to the value of the control using the medium with no sample added.

As can be seen from Table 5, when a medium supplemented with the oyster family plant extract was used, a decrease in the amount of melanin production was observed. From this, it has been found that the spring-like plant extract has an excellent tyrosinase activity inhibitory action.
From the above, it has been clarified that the spring family plant extract has an excellent whitening effect.

<Evaluation of neutral fat accumulation inhibitory action>
Evaluation of the neutral fat accumulation inhibitory action in the adipocytes of the springfish plant extract was performed by the following method. As a sample, a genus plant extract produced by Preparation Method 1 was used.
Subcutaneous fat-derived normal human preadipocytes Cryo • HPRAD-SQ (Sanko Junyaku Co., Ltd.) were seeded on a 96-well microplate so that the number was 1.0 × 10 ⁴ per well. PGM medium (containing 10% by mass fetal bovine serum (FBS), 2 mM L-Glutamine, 100 units / mL Penicilline, 100 μg / mL Streptomycine) was used as the seeding medium. Immediately before the cells are saturated, the medium is changed to PGM differentiation medium (containing 10 μg / mL insulin, 1 μM dexamethasone, 200 μM indomethacin, 500 μM Isobutyl-methylxanthine) to which a sample having a concentration shown in Table 6 is added, and induction of differentiation into adipocytes Went. After initiation of differentiation induction, the cells were cultured for 10 to 14 days until the control group matured and many lipid droplets accumulated in the cells. After the cells were collected, the cells were fixed using a 10% neutral buffered formaldehyde solution. After washing with PBS, 0.5 w / v% Oil Red O solution was added and incubated at 37 ° C. for 2 hours. After washing with PBS, methanol was added to extract the dye. After extraction, the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the amount of accumulated triglyceride was measured using the difference between the two measured values.
The measurement results are shown in Table 6 in terms of relative values with the neutral fat accumulation amount being 100 in the control using the medium without the sample.

  As can be seen from Table 6, a significant neutral fat accumulation inhibitory effect was observed in the medium supplemented with the oyster family plant extract. From this, it has been clarified that the springfish plant extract has an excellent neutral fat accumulation inhibitory action.

<Evaluation of antioxidant action 1 (radical scavenging action)>
Evaluation of the antioxidant effect of the springfish plant extract was performed by the following method. As a sample, a genus plant extract produced by Preparation Method 1 was used.
The concentration of each sample was adjusted to 50% by mass ethanol to obtain a sample solution, and 100 μL each was added to a 96-well microplate so that the concentrations shown in Table 7 were obtained. Thereto, 100 μL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution was added, mixed well, and allowed to stand at room temperature in the dark for 24 hours. Thereafter, the absorbance at 516 nm derived from the DPPH radical was measured. When the absorbance of the control when the sample was not added was (A) and the absorbance when the sample was added was (B), the elimination rate of the DPPH radical was introduced into Equation (2) and obtained. The results are shown in Table 7.
Formula (2): Radical scavenging rate = {1- (B) / (A)} × 100 (%)

  As is clear from Table 7, an excellent DPPH radical scavenging effect was observed in the genus genus plant extract. From this, it has been found that the spring-like plant extract has an excellent radical scavenging action.

<Evaluation of antioxidative action 2 (superoxide anion scavenging action)>
Evaluation of the superoxide anion scavenging action of the Amaranthaceae plant extract was performed by the following method. As a sample, a genus plant extract produced by Preparation Method 1 was used.
In a 96-well microplate, 25 μL of a Hanks (+) solution of a spring extract diluted to an arbitrary concentration to a concentration shown in Table 8, and 75 μL of a reaction solution (0.25 mM NBT, 1 mM Hypoxanthine, 0.1 mM EDTA) Containing Hanks (+) solution (pH 7.4)), 25 μL of xanthine oxidase solution (0.0075 Units) was added, and the mixture was incubated at 37 ° C. for 15 minutes. Thereafter, the absorbance at 540 nm was measured with a microplate reader. When the absorbance of the control without addition of the sample was (A) and the absorbance when the sample was added was (B), the value of formula (3) was defined as the radical elimination rate. The evaluation results are shown in Table 8.
Formula (3): {1- (B) / (A)} × 100 (%)

As can be seen from Table 8, the superior extract of superoxide anion was recognized in the Amaranthaceae plant extract. From this, it was found that the spring-like plant extract has an excellent superoxide anion scavenging action.
From the above, it has been clarified that the oyster family plant extract has an excellent antioxidant action.

製法：（１）〜（６）の油相成分を８０℃にて加熱溶解する。一方（７）〜（10）の水相成分を８０℃にて加熱溶解する。これに前記油相成分を攪拌しながら加え、ホモジナイザーにより均一に乳化する。乳化終了後、冷却を開始し、（11）と（12）を順次加え、均一に混合する。Then, the formulation example of the skin external preparation which mix | blended the spring-like plant extract obtained by said each preparation method is shown.
[Example 1] Emulsion formulation:

Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

製法：（１）に（２）及び（３）を溶解する。溶解後、（４）〜（８）を順次添加した後、十分に攪拌し、（９）を加え、均一に混合する。[Example 2] Lotion formulation:

Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

製法：（１）〜（６）の油相成分を８０℃にて加熱溶解する。一方（７）〜（10）の水相成分を８０℃にて加熱溶解する。これに前記油相成分を攪拌しながら加え、ホモジナイザーにより均一に乳化する。乳化終了後、（11）を加え、冷却を開始し、４０℃にて（12）を加え、均一に混合する。[Example 3] Cream formulation:

Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

製法：（１）〜（６）の水相成分を混合し、７５℃にて加熱溶解する。一方、（７）〜（14）の油相成分を混合し、７５℃にて加熱溶解する。次いで、上記水相成分に油相成分を添加して予備乳化を行った後、ホモミキサーにて均一に乳化する。乳化終了後に冷却を開始し、５０℃にて（15）を加える。さらに４０℃まで冷却し、（16）を加え、均一に混合する。[Example 4] Cosmetic liquid formulation:

Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

製法：（１）を（２）に加え、均一に攪拌した後、（３）を加える。均一に攪拌した後、（４）に予め溶解させた（５）を加える。均一に攪拌した後、予め混合しておいた（６）〜（８）を加え、均一に攪拌混合する。[Example 5] Aqueous gel formulation:

Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

製法：（１）と（２）を均一に溶解する。これに、（３）と（４）を順次加え、均一に混合する。[Example 6] Formulating cleansing material:

Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

製法：（１）〜（４）の油相成分を８０℃にて加熱溶解する。一方（５）〜（７）の水相成分を８０℃にて加熱溶解し、油相成分と均一に混合撹拌する。冷却を開始し、４０℃にて（８）を加え、均一に混合する。[Example 7] Facial cleansing foam formulation:

Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

製法：（１）〜（４）の油相成分を混合し、７５℃にて加熱溶解する。一方、（５）〜（７）の水相成分を混合し、７５℃にて加熱溶解し、これに（８）〜（10）の顔料を加え、ホモミキサーにて均一に分散させる。この水相成分に前記油相成分を加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、４０℃にて（11）と（12）の成分を加え、均一に混合する。[Example 8] Makeup base cream formulation:

Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and uniformly dispersed with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

製法：（１）〜（６）の油相成分を混合し、７５℃にて加熱溶解する。一方、（７）〜（10）の水相成分を混合し、７５℃にて加熱溶解し、これに（11）〜（15）の顔料を加え、ホモミキサーにて均一に分散する。油相成分を加え、乳化を行う。乳化終了後に冷却を開始し、４０℃にて（16）と（17）の成分を順次加え、均一に混合する。[Example 9] Emulsion foundation formulation:

Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

製法：（５）と（６）を（11）の一部に溶解して５０℃とし、５０℃に加熱した（４）に撹拌しながら徐々に加える。これを混合した後、７０℃にて加熱溶解した（１）〜（３）に均一に分散する。これに、（７）〜（10）を（11）の残部に７０℃にて加熱溶解したものを撹拌しながら加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、４０℃にて（12）を加え、均一に混合する。[Example 10] Formulation of water-in-oil emollient cream:

Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring, and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

製法：（２）と（３）を混合し、８０℃に加温した後、８０℃に加温した（１）に溶解する。均一に溶解した後、（４）と（５）を加え、攪拌しながら冷却を開始する。４０℃まで冷却し、（６）と（７）を加え、均一に混合する。[Example 11] Pack formulation:

Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

製法：（１）〜（４）を均一に混合する。[Example 12] Bath additive formulation:

Production method: (1) to (4) are mixed uniformly.

製法：（１）〜（６）の油相成分を混合し、７５℃にて加熱溶解後する。一方、（７）〜（10）の水相成分を７５℃にて加熱溶解し、前記油相成分を加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、４０℃にて（11）と（12）の成分を加え、均一に混合する。[Example 13] Hair wax formulation:

Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

製法：（１）〜（４）の成分を混合、均一化する。[Example 14] Hair art formulation:

Production method: Components (1) to (4) are mixed and homogenized.

<Evaluation of moisturizing action 1 (humidity retention)>
A use test using the external preparation for skin of Examples 1 and 3 was conducted, and the moisturizing property of the spring extract was evaluated. At that time, the genus plant extract blended in Comparative Example 1 and Example 3 in which the 50-mass ethanol aqueous solution blended in Example 1 was replaced with the 50% -mass ethanol aqueous solution was replaced with a 50% mass ethanol aqueous solution. The test was conducted in the same manner as Comparative Example 2 as a test sample.

  A group of 15 male and female panelists was used, and each group was blindly used for one month for each group of 4 panelists, and evaluated in three stages: “feel”, “cannot say”, and “do not feel”. Table 9 shows the number of panelists that obtained each evaluation.

  From Table 9, it was found that a clear moisture retention was felt by 80% or more of the panelists in the examples using group in which the spring plant extract was blended. From this, it was clarified that the spring-like plant extract has an excellent moisturizing action.

<Evaluation 2 of moisturizing action (rough skin, texture of skin, transparency of skin)>
The use test using the skin external preparations of Examples 1 and 3 and Comparative Examples 1 and 2 was conducted, and the improvement effect of those symptoms was evaluated with respect to rough skin, skin texture, and skin transparency.

  For each sample, a group of 20 male and female panelists in their 20s to 50s who are worried about rough skin, skin texture, and skin transparency, let each group use the samples blindly for 1 month, Changes in skin condition before and after use were observed and evaluated. As indicators of skin condition, rough skin, skin texture, and skin transparency were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. The rough skin and the transparency of the skin were visually evaluated, and the texture of the skin was observed using a microscope. Table 10 shows the number of panelists that obtained each evaluation.

From Table 10, in terms of rough skin, skin texture, and skin transparency, in Comparative Examples 1 and 2 using groups that did not contain the spring family plant extract, more than half of the panelists showed no improvement. In the example use group in which the extract was blended, a clear improvement was observed in 70% or more of panelists. From this, it was found that the spring-like plant extract has an excellent effect in improving skin symptoms in rough skin, skin texture, and skin transparency.
From the above, it has been clarified that the spring-like plant extract has an excellent moisturizing action.

Claims (6)

A moisturizing agent comprising one or more plants selected from Osmundaceae plants or an extract thereof as an active ingredient. A cell activator comprising, as an active ingredient, one or two or more kinds of plants selected from the family Osmundaceae or extracts thereof. A whitening agent comprising one or more plants selected from Osmundaceae plants or an extract thereof as an active ingredient. A neutral fat accumulation inhibitor comprising as an active ingredient one or more plants selected from Osmundaceae plants or an extract thereof. An antioxidant comprising one or more plants selected from Osmundaceae plants or an extract thereof as an active ingredient. A skin external preparation characterized by containing one or two or more plants selected from Osmundaceae plants or extracts thereof.