KR102206094B1 - Cosmetic composition comprising Pharbitidis Semen extract - Google Patents

Abstract

The present invention relates to a composition for inhibiting peritoneal mesothelial cell death comprising alpha-1 antitrypsin, and more particularly, to a composition for inhibiting peritoneal mesothelial cell death by inhibiting caspase-3, alpha- 1 It relates to a composition for inhibiting peritoneal mesothelial cell death comprising antitrypsin as an active ingredient.
The cosmetic composition comprising the extract of Gyeonwooja provided by the present invention as an active ingredient exhibits an excellent antioxidant effect and wrinkle improvement effect, and thus can be very usefully used in the development of a cosmetic that recovers skin aging caused by various internal and external factors.

Description

Cosmetic composition comprising Pharbitidis Semen extract}

The present invention relates to a cosmetic composition comprising an extract of Gyeonwooja, and more particularly, to a cosmetic composition for improving wrinkles or antioxidants, comprising a Gyeonwooja extract having excellent DPPH active oxygen scavenging activity and collagenase inhibitory activity as an active ingredient.

The skin is largely composed of the epidermal layer, the dermis layer, and the subcutaneous fat layer, and the most basic role is to prevent the evaporation of moisture from our body and to prevent the invasion of harmful ingredients from the outside. Since such skin is always exposed from external stimuli, it has various defense capabilities. In addition, as the skin is a sign of beauty, it can be said that healthy and clean skin is directly related to human beauty.

Human skin undergoes various physical and chemical changes during the aging process. The causes are largely divided into intrinsic aging and photo-aging, and studies on this have been actively conducted. UV rays, stress, disease conditions, environmental factors, wounds, and aging can be caused by activation of reactive oxygen species.If such a condition intensifies, it destroys the antioxidant defenses existing in the living body and damages cells and tissues, leading to adult diseases. And promotes aging. More specifically, skin cells and tissues are destroyed by oxidation of lipids, proteins, polysaccharides and nucleic acids, which are major components of the skin, resulting in skin aging. In particular, the oxidation of proteins cuts the connective tissues of the skin, such as collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, which causes severe excessive inflammatory reactions and disrupts skin elasticity. If this becomes more severe, mutations in the DNA lead to mutations, cancer, and immune function decline.

Therefore, free radicals generated during the body's metabolic process, UV irradiation, and active oxygen mediated by inflammatory reactions are eliminated to protect the cell membrane, and cells that have already been damaged must be regenerated by active metabolism to proliferate cells. It recovers quickly and can maintain healthy skin.

As human skin ages, wrinkles naturally occur on the skin. However, when such wrinkles are exposed to ultraviolet rays or exposed to external pollutants for a long time, the degree of formation is accelerated and wrinkles become deeper and more numerous. The biggest causes of wrinkles are decreased collagen synthesis and decomposition. As a method for inhibiting wrinkle formation, there are generally methods such as inhibiting wrinkle formation by promoting collagen synthesis, or inhibiting wrinkle formation by inhibiting the production or activity of collagen-degrading enzymes.

In order to prevent skin aging, various attempts have been made, such as laser treatment and face shaping, but as a conventional patent related to an anti-aging cosmetic composition using natural extracts rather than an artificial method for aging as described above, Korean Patent Publication No. 10-0562348 B1 "High-functional cosmetic composition containing whitening, wrinkle, and anti-aging cosmetic ingredients containing ethanol extract of Ephedra root", Korean Patent Publication 10-0588830 B1 "Anti-aging cosmetic composition containing white rhizome extract", Korean Registered Patent Publication 10-0443588 B1 ``Anti-aging cosmetic composition containing Mokdan skin extract and Albizia julibrissin extract'', Korean Patent Publication 10-0536744 B1 ``Cosmetic composition having anti-aging effect on skin and its manufacturing method'', etc. There are many of these. However, there is not yet a literature report on the skin aging improvement effect of Gyeonwooja extract, and the optimal preparation method of Gyeonwooja extract to exert skin antioxidant and wrinkle improvement effects has not been studied yet.

Accordingly, the present inventors have conducted intensive research to develop a cosmetic composition for anti-oxidation and skin wrinkle improvement using a new natural extract, and as a result, Gyeonwooja extract exhibits excellent DPPH active oxygen scavenging activity and collagenase inhibitory activity to improve antioxidant and wrinkle improvement. It was discovered that it could be utilized as a composition for use, and the present invention was completed.

Accordingly, an object of the present invention is to provide a cosmetic composition for improving wrinkles comprising the extract of Gyeonwooja as an active ingredient.

Another object of the present invention is to provide an antioxidant composition comprising the extract of Gyeonwooja as an active ingredient.

In order to achieve the above object of the present invention, the present invention provides a cosmetic composition for improving wrinkles comprising the extract of Gyeonwooja as an active ingredient.

In order to achieve another object of the present invention, the present invention provides an antioxidant composition comprising an extract of Gyeonwooja as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition for improving wrinkles comprising the extract of Gyeonwooja as an active ingredient.

The term “gyeonwooja” used in the present invention refers to the seeds of the morning glory of the convolvulaceae family (Pharbitis nil Choisy), and is called Pharbitidis Semen by its scientific name, and also called heukchuk (黑丑) as the name of herbal medicine. Morning glory flowers are native to tropical Asia such as India, and about 256 varieties are known, and black seeds are called black axes and white seeds are called baekchuk. Gyeonwooja has strong saha (瀉下) and diuretic effects, and is used when the body is swollen, chronic pyelonephritis, cirrhosis, etc. In addition, it is effective against seawater and asthma, and is also used to remove constipation and parasites. As a pharmacological action, a strong saha action due to intestinal peristalsis has been reported. Gyeonwooja contains about 2% of parbitin, a kind of resinous polysaccharide, and 11% of fatty acids such as olein, palmitin, and stearin.

When the extract of Gyeonwooja used in the composition of the present invention is obtained by treating Gyeonwooja with an extraction solvent, various extraction solvents may be used. Preferably, a polar solvent or a non-polar solvent can be used. Suitable as polar solvents are (i) water, (ii) alcohols (preferably methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol. Or ethylene glycol), (iii) acetic acid, (iv) DMFO (dimethylformamide) and (v) DMSO (dimethyl sulfoxide). Suitable non-polar solvents include acetone, acetona itryl, ethyl acetate, methyl acetate, fluoroalkane, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1 -Pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, Dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and THF. Most preferably, the extract of Gyeonwooja in the present invention may be a water extract.

The term'extract' as used herein has a meaning commonly used as a crude extract in the art, but broadly includes a fraction obtained by further fractionating the extract. That is, the extract of Gyeonwooja includes not only those obtained by using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, fractions obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through the purification method are also included in the extract of the present invention.

According to an embodiment of the present invention, it was confirmed that the water extract of Gyeonwooja was remarkably excellent in the inhibitory activity of collagenase compared to the 70% ethanol extract.

Gyeonwooja extract used in the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In the present specification, the term "including as an active ingredient" means including an amount sufficient to achieve the efficacy or activity of the following Gyeonwooja extract. The present invention is a composition extracted from the natural plant material, Gyeonwooja, and there is no side effect on the human body even if an excessive dose is administered. Therefore, the upper limit of the quantity contained in the composition of the present invention can be carried out by those skilled in the art by selecting within an appropriate range.

According to an embodiment of the present invention, the extract of Gyeonwooja was found to be remarkably superior in collagenase inhibitory activity compared to other natural extracts, and thus it was confirmed that it can be very useful as a cosmetic composition for wrinkle improvement.

On the other hand, when the cosmetic composition for wrinkle improvement of the present invention is provided in the form of a single extract of Gyeonwooja rather than a complex extract with other natural products, the best effect can be exerted, and preferably provided in the form of a hot water extract of Gyeonwooja alone. In this case, most preferably, the freeze-dried powder of the hot water extract of Gyeonwooja alone may be provided in a form containing at a concentration of 200ug/ml or more.

According to the present invention, the gyeonwooja extract is contained in 0.0001 to 30.0% by weight with respect to the total cosmetic composition, more preferably, the gyeonwooja extract is characterized in that it contains 0.01 to 10% by weight with respect to the entire cosmetic composition. When the content of the extract is less than 0.0001% by weight, the skin wrinkle improvement effect does not appear, and when it exceeds 30.0% by weight, the degree of increase in the skin improvement effect due to the increase in content is insignificant, and there is a problem in safety and stability of the formulation. It is also not economical.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredients, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and It includes a carrier.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, spray, etc. may be formulated, but is not limited thereto. In more detail, it may be prepared in the form of a flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

According to another embodiment of the present invention, it has been confirmed that the extract of Gyeonwooja is excellent in scavenging DPPH active oxygen and can be used as an antioxidant cosmetic composition.

Accordingly, the present invention provides an antioxidant cosmetic composition comprising the extract of Gyeonwooja as an active ingredient.

Unlike the anti-wrinkle effect, the anti-oxidant effect of the extract of Gyeonwooja was confirmed to exhibit very excellent effects in both the hot water extract and the 70% ethanol extract. As described above, it can be expected that the optimal extraction solvents exhibiting antioxidant effect and wrinkle improvement effect are partially different despite the same Gyeonwooja extract, because the active ingredients exhibiting each effect are different, and such findings have not been previously reported. As such, the present inventors disclose for the first time through the present invention.

In the present invention, the "antioxidation" refers to free radicals generated during the metabolic process of the body, ultraviolet irradiation by the sun, and free radicals generated by inflammatory reactions to protect cell membranes, and furthermore, to protect cell membranes, which have already been damaged by active oxygen It refers to the action of regenerating cells so that they can be restored to healthy skin.

Free radicals act on the skin and promote melanin production, causing pigmentation (stains, freckles, etc.), as well as various forms of skin aging (e.g., wrinkles, dry skin, loss of elasticity, etc.). The antioxidant cosmetic composition of the present invention can be used to recover various types of skin damage, that is, to improve skin conditions.

The description related to the extract and the cosmetic composition may be applied in the same manner as described in the cosmetic composition for improving wrinkles.

The cosmetic composition comprising the extract of Gyeonwooja provided by the present invention as an active ingredient exhibits an excellent antioxidant effect and wrinkle improvement effect, and thus can be very usefully used in the development of a cosmetic that recovers skin aging caused by various internal and external factors.

1 is a graph showing the DPPH active oxygen scavenging ability of L-ascorbic acid (positive control), hot water extract of Gyeonja and 70% ethanol extract of Gyunja in percentage compared to the untreated control.
Figure 2 is a graph showing the collagenase inhibitory activity of EGCG (positive control), hot water extract and 70% ethanol extract of cow cow in percentage compared to untreated control group.

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are for illustrative purposes only, and the present invention is not limited thereto.

1. Test substance

Gyeonwooja hot water extract used in the experiment was extracted with hot water for 2 hours by adding 10 times of distilled water to 200 g of Gyeonwooja, filtered, concentrated with a vacuum concentrator (Buchi B-4580, Switzerland), and then completely dried using a freeze dryer. The obtained powder was used in the experiment. The yield of the hot water extract of Gyeonwooja was 2.55%.

The 70% ethanol extract of Gyeonwooja was added with 70% ethanol to 100 g of Gyeonwooja, followed by cold-spit extraction for 24 hours, concentrated with a vacuum concentrator (Buchi B-4580, Switzerland), and then completely dried using a freeze dryer. It was diluted with distilled water and used in the experiment. The yield of the 70% ethanol extract of Gyeonwooja was 5%.

2. Example 1: DPPH active oxygen scavenging activity measurement

DPPH was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA), and L-ascorbic acid was used as a positive control. 100 μL of a solution obtained by diluting the positive control group and the prepared Gyeonwooja extract by concentration and 100 μL of a 60 μM DPPH solution were mixed and reacted in a dark state at room temperature for 30 minutes, and the absorbance was measured at 540 nm. DPPH radical scavenging activity was expressed as a percentage of the scavenging degree of DPPH active oxygen compared to the control without adding the sample.

The results are shown in FIG. 1.

As shown in FIG. 1, the inhibition rate at 100 μg/mL was 97.86 ± 0.08 for the control L-ascorbic acid, 90.98 ± 1.28 for Gyeonwooja hot water extract, and 75.81 ± 2.79 for Gyeonwooja 70% ethanol extract. DPPH radical scavenging activity was found to be higher in hot water extract than ethanol extract.

3. Example 2: Collagenase (collagenase) inhibitory activity evaluation

50 μL of 2units/mL collagenase and 15 μL of each concentration of Gyeonwooja extract sample were added to 35 μL of 50 mM tricine buffer at pH 7.5, and then allowed to stand for 15 minutes. Thereafter, 50 μL of 2 mM FALGPA (N-(3-[2-Furyl] Acryloyl)-Leu-Gly-Pro-Ala) was added and the absorbance was immediately measured at 345 nm. EGCG (Epigallocatechin gallate) was used as a positive control. The collagenase inhibitory activity was expressed as a percentage of the degree of collagenase inhibition compared to the control without adding the sample.

The results are shown in FIG. 2.

As shown in FIG. 2, the inhibition rate at 200 μg/mL was 44.21 ± 1.45 for the control EGCG, 23.12 ± 0.76 for the hot water extract of Gyeonwooja, and 8.04 ± 0.37 for the 70% ethanol extract of Gyeonwooja. Collagenase inhibitory activity was higher in hot water extract than in Gyeonwooja 70% ethanol extract.

4. Comparative Example 1: Evaluation of collagenase inhibitory activity of extracts from other natural products

After confirming that the hot water extract of Gyeonwooja in the above example has excellent collagenase inhibitory activity, ginseng, multi-leaf, licorice, astragalus, and noshoe were used in the same method as the method of preparing Gyunwooja hot-water extract in the preparation step of the “test substance”. (Aloe) and hot water extracts of a mixture thereof were prepared, respectively.

The collagenase inhibitory activity of each of the prepared natural hot water extracts and their complex extracts was performed in the same manner as in Example 2.

The results are shown in Table 1 below.

As can be seen in Table 1, it was confirmed that the collagenase inhibitory activity of the extracts of ginseng, multileaf, licorice, astragalus, nohoe or their complex hot water was significantly lower compared to the extract of Gyeonwooja of the same concentration.

The cosmetic composition provided by the present invention containing the extract of Gyeonwooja as an active ingredient exhibits excellent antioxidant effect and wrinkle improvement effect and can be very usefully used in the development of cosmetics that restore skin aging caused by various internal and external factors. The availability of the award is very good.

Claims (4)

A cosmetic composition for improving wrinkles consisting of Gyeonwooja hot water extract. delete delete delete

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