JP2005538719A5 - - Google Patents
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- Publication number
- JP2005538719A5 JP2005538719A5 JP2004536265A JP2004536265A JP2005538719A5 JP 2005538719 A5 JP2005538719 A5 JP 2005538719A5 JP 2004536265 A JP2004536265 A JP 2004536265A JP 2004536265 A JP2004536265 A JP 2004536265A JP 2005538719 A5 JP2005538719 A5 JP 2005538719A5
- Authority
- JP
- Japan
- Prior art keywords
- reverse transcriptase
- mlv
- synthesized
- full
- length cdna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102100034343 Integrase Human genes 0.000 claims 45
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 45
- 239000002299 complementary DNA Substances 0.000 claims 12
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims 3
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims 3
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 3
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 3
- 239000004472 Lysine Substances 0.000 claims 3
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims 3
- 239000004473 Threonine Substances 0.000 claims 3
- 150000001413 amino acids Chemical class 0.000 claims 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 3
- 229930182817 methionine Natural products 0.000 claims 3
- 238000012986 modification Methods 0.000 claims 3
- 230000004048 modification Effects 0.000 claims 3
- 230000035772 mutation Effects 0.000 claims 3
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims 3
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims 3
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41028302P | 2002-09-13 | 2002-09-13 | |
| PCT/US2003/028802 WO2004024749A2 (en) | 2002-09-13 | 2003-09-15 | Thermostable reverse transcriptases and uses thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005538719A JP2005538719A (ja) | 2005-12-22 |
| JP2005538719A5 true JP2005538719A5 (https=) | 2006-11-02 |
| JP4634799B2 JP4634799B2 (ja) | 2011-02-16 |
Family
ID=31994103
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004536265A Expired - Lifetime JP4634799B2 (ja) | 2002-09-13 | 2003-09-15 | 耐熱性逆転写酵素およびその使用法 |
Country Status (5)
| Country | Link |
|---|---|
| US (4) | US20040209276A1 (https=) |
| EP (2) | EP1554377B1 (https=) |
| JP (1) | JP4634799B2 (https=) |
| AU (1) | AU2003274973A1 (https=) |
| WO (1) | WO2004024749A2 (https=) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9771565B2 (en) | 2000-05-26 | 2017-09-26 | Life Technologies Corporation | Thermostable reverse transcriptases and uses thereof |
| US20040152072A1 (en) * | 2002-07-30 | 2004-08-05 | Invitrogen Corporation | Reverse transcription |
| JP4634799B2 (ja) | 2002-09-13 | 2011-02-16 | ライフ テクノロジーズ コーポレーション | 耐熱性逆転写酵素およびその使用法 |
| US7595179B2 (en) | 2004-04-19 | 2009-09-29 | Applied Biosystems, Llc | Recombinant reverse transcriptases |
| US9783791B2 (en) | 2005-08-10 | 2017-10-10 | Agilent Technologies, Inc. | Mutant reverse transcriptase and methods of use |
| JP5203200B2 (ja) * | 2005-08-10 | 2013-06-05 | アジレント・テクノロジーズ・インク | 変異体逆転写酵素および使用方法 |
| US8374838B2 (en) * | 2007-10-22 | 2013-02-12 | The Wistar Institute | Method for identifying a compound that modulates telomerase activity |
| GB0806562D0 (en) * | 2008-04-10 | 2008-05-14 | Fermentas Uab | Production of nucleic acid |
| IN2012DN05145A (https=) | 2009-11-19 | 2015-10-23 | Solis Biodyne | |
| KR101818126B1 (ko) * | 2011-02-09 | 2018-01-15 | (주)바이오니아 | 열안정성이 증가된 역전사효소 |
| US11465785B1 (en) * | 2012-11-29 | 2022-10-11 | David M. Dodgen | Quick filling and self sealing fluid storage bag assembly |
| JP6221374B2 (ja) * | 2012-12-27 | 2017-11-01 | 東ソー株式会社 | 熱安定性が向上したトリ骨髄芽細胞腫ウイルス逆転写酵素 |
| US9353409B2 (en) * | 2013-01-15 | 2016-05-31 | Jun Euihum Lee | Compositions and methods for RT-PCR |
| DK3043827T3 (da) * | 2013-09-13 | 2019-08-26 | Ionis Pharmaceuticals Inc | Modulatorer af komplement faktor b |
| US9920305B2 (en) * | 2013-10-16 | 2018-03-20 | New England Biolabs, Inc. | Reverse transcriptase with enhanced properties |
| EP3097190A2 (en) | 2014-01-22 | 2016-11-30 | Life Technologies Corporation | Novel reverse transcriptases for use in high temperature nucleic acid synthesis |
| WO2017178986A1 (en) * | 2016-04-12 | 2017-10-19 | Solis Biodyne Oü | Synthetic reverse transcriptases and uses thereof |
| EP3940066A1 (en) * | 2016-09-23 | 2022-01-19 | New England Biolabs, Inc. | A mutant reverse transcriptase |
| KR102709829B1 (ko) * | 2016-11-11 | 2024-09-24 | 바이오 래드 래버러토리스 인코오포레이티드 | 핵산 샘플을 프로세싱하는 방법 |
| US11220677B2 (en) | 2016-12-14 | 2022-01-11 | Takara Bio Inc. | Heat-resistant reverse transcriptase mutant |
| WO2018136404A1 (en) | 2017-01-19 | 2018-07-26 | Asuragen, Inc. | Methods of rna amplification |
| US10988762B2 (en) | 2017-02-16 | 2021-04-27 | Bio-Rad Laboratories, Inc. | Reverse transcriptases and uses thereof |
| US20180312822A1 (en) | 2017-04-26 | 2018-11-01 | 10X Genomics, Inc. | Mmlv reverse transcriptase variants |
| US11802311B2 (en) | 2018-03-15 | 2023-10-31 | Massachusetts Institute Of Technology | Methods of quantifying RNA and DNA variants through sequencing employing phosphorothioates |
| WO2019222523A2 (en) * | 2018-05-16 | 2019-11-21 | Bio-Rad Laboratories, Inc. | Methods for processing nucleic acid samples |
| EP3805380B1 (en) | 2019-10-07 | 2025-12-03 | Qiagen Beverly, LLC | Improved thermostable viral reverse transcriptase |
| EP4073241A2 (en) | 2019-12-11 | 2022-10-19 | 10X Genomics, Inc. | Reverse transcriptase variants |
| CN116547379A (zh) | 2020-07-20 | 2023-08-04 | 综合基因技术公司 | 具有增加的活性和热稳定性的逆转录酶突变体 |
| US12559734B2 (en) | 2020-07-20 | 2026-02-24 | Integrated Dna Technologies, Inc. | Reverse transcriptase mutants with increased activity and thermostability |
| CN113174381B (zh) * | 2021-06-08 | 2023-05-12 | 翌圣生物科技(上海)股份有限公司 | 增强型mmlv逆转录酶突变体及其应用 |
| WO2023235685A1 (en) * | 2022-06-01 | 2023-12-07 | Bio-Rad Laboratories, Inc. | Method and system for detecting reverse transcriptase activity by digital assay |
| WO2024064136A1 (en) * | 2022-09-19 | 2024-03-28 | Domus Diagnostics, Inc. | Rapid molecular diagnostics with unified-one-pot sample processing, nucleic acid amplification, and result readout |
Family Cites Families (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4663290A (en) | 1982-01-21 | 1987-05-05 | Molecular Genetics, Inc. | Production of reverse transcriptase |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| ATE68524T1 (de) | 1985-07-09 | 1991-11-15 | Quadrant Bioresources Ltd | Beschuetzung von proteinen und aehnlichem. |
| US5017492A (en) | 1986-02-27 | 1991-05-21 | Life Technologies, Inc. | Reverse transcriptase and method for its production |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| WO1992006200A1 (en) | 1990-09-28 | 1992-04-16 | F. Hoffmann-La-Roche Ag | 5' to 3' exonuclease mutations of thermostable dna polymerases |
| US4962022A (en) | 1986-09-22 | 1990-10-09 | Becton Dickinson And Company | Storage and use of liposomes |
| US5244797B1 (en) * | 1988-01-13 | 1998-08-25 | Life Technologies Inc | Cloned genes encoding reverse transcriptase lacking rnase h activity |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5498523A (en) | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
| GB8903593D0 (en) | 1989-02-16 | 1989-04-05 | Pafra Ltd | Storage of materials |
| DE553264T1 (de) | 1990-10-05 | 1994-04-28 | Wayne M Barnes | Thermostabile dna polymerase. |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5646016A (en) | 1991-02-06 | 1997-07-08 | Genetics Institute, Inc. | Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules |
| IL103267A (en) | 1991-09-24 | 2004-07-25 | Keygene Nv | Process and kit for amplification of restriction fragment obtained from starting dna |
| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| US5912155A (en) | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
| US6015668A (en) | 1994-09-30 | 2000-01-18 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga and mutants thereof |
| US5556771A (en) | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
| EP0871775A4 (en) | 1995-09-08 | 2002-07-31 | Life Technologies Inc | CLONED DNA POLYMERASES FROM THERMOTOGA AND MUTANTS THEREOF |
| US5688005A (en) | 1996-07-10 | 1997-11-18 | Baldwin Hardware Corporation | Door knob spindle with spring |
| JP2000516468A (ja) | 1996-08-14 | 2000-12-12 | ライフ テクノロジーズ，インコーポレイテッド | 核酸増幅および配列決定のための安定な組成物 |
| WO1998023733A2 (en) | 1996-11-27 | 1998-06-04 | University Of Washington | Thermostable polymerases having altered fidelity |
| EP2184289A1 (en) | 1997-04-22 | 2010-05-12 | Life Technologies Corporation | Methods for the production of ASLV reverse transcriptases composed of multiple subunits |
| JP2002502237A (ja) | 1997-05-12 | 2002-01-22 | ライフ テクノロジーズ，インコーポレイテッド | 核酸分子の生成および精製のための方法 |
| EP2390258A1 (en) | 1997-08-29 | 2011-11-30 | Life Technologies Corporation | High fidelity polymerases and uses thereof |
| US6399334B1 (en) | 1997-09-24 | 2002-06-04 | Invitrogen Corporation | Normalized nucleic acid libraries and methods of production thereof |
| US6136582A (en) * | 1998-01-20 | 2000-10-24 | The Trustees Of Columbia University In The City | Reverse transcriptase of moloney murine leukemia virus with RNA polymerase activity |
| US6183998B1 (en) | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
| JP4193079B2 (ja) | 1998-11-10 | 2008-12-10 | 東洋紡績株式会社 | 変異型逆転写酵素 |
| CN100390275C (zh) | 2000-03-15 | 2008-05-28 | 茵维特罗根公司 | 高保真逆转录酶及其用途 |
| CN1430670A (zh) * | 2000-05-26 | 2003-07-16 | 茵维特罗根公司 | 热稳定逆转录酶及其用途 |
| US7078208B2 (en) * | 2000-05-26 | 2006-07-18 | Invitrogen Corporation | Thermostable reverse transcriptases and uses thereof |
| EP1301205A1 (en) * | 2000-07-12 | 2003-04-16 | Invitrogen Corporation | High fidelity polymerases and uses thereof |
| JP4634799B2 (ja) | 2002-09-13 | 2011-02-16 | ライフ テクノロジーズ コーポレーション | 耐熱性逆転写酵素およびその使用法 |
| GB0806562D0 (en) | 2008-04-10 | 2008-05-14 | Fermentas Uab | Production of nucleic acid |
-
2003
- 2003-09-15 JP JP2004536265A patent/JP4634799B2/ja not_active Expired - Lifetime
- 2003-09-15 AU AU2003274973A patent/AU2003274973A1/en not_active Abandoned
- 2003-09-15 US US10/661,819 patent/US20040209276A1/en not_active Abandoned
- 2003-09-15 WO PCT/US2003/028802 patent/WO2004024749A2/en not_active Ceased
- 2003-09-15 EP EP03759243A patent/EP1554377B1/en not_active Expired - Lifetime
- 2003-09-15 EP EP10178740.6A patent/EP2325303B1/en not_active Expired - Lifetime
-
2006
- 2006-07-25 US US11/492,194 patent/US20070141592A1/en not_active Abandoned
-
2010
- 2010-12-28 US US12/979,899 patent/US8753845B2/en not_active Expired - Lifetime
-
2014
- 2014-05-07 US US14/272,433 patent/US9862938B2/en not_active Expired - Fee Related