AU2003274973A1 - Thermostable reverse transcriptases and uses thereof - Google Patents

Thermostable reverse transcriptases and uses thereof

Info

Publication number
AU2003274973A1
AU2003274973A1 AU2003274973A AU2003274973A AU2003274973A1 AU 2003274973 A1 AU2003274973 A1 AU 2003274973A1 AU 2003274973 A AU2003274973 A AU 2003274973A AU 2003274973 A AU2003274973 A AU 2003274973A AU 2003274973 A1 AU2003274973 A1 AU 2003274973A1
Authority
AU
Australia
Prior art keywords
reverse transcriptases
thermostable reverse
thermostable
transcriptases
reverse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003274973A
Other languages
English (en)
Other versions
AU2003274973A8 (en
Inventor
Gulshan Dhariwal
Gary F. Gerard
Jun E. Lee
Robert Jason Potter
Kim Rosenthal
Michael D. Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of AU2003274973A1 publication Critical patent/AU2003274973A1/en
Publication of AU2003274973A8 publication Critical patent/AU2003274973A8/xx
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2003274973A 2002-09-13 2003-09-15 Thermostable reverse transcriptases and uses thereof Abandoned AU2003274973A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41028302P 2002-09-13 2002-09-13
US60/410,283 2002-09-13
PCT/US2003/028802 WO2004024749A2 (en) 2002-09-13 2003-09-15 Thermostable reverse transcriptases and uses thereof

Publications (2)

Publication Number Publication Date
AU2003274973A1 true AU2003274973A1 (en) 2004-04-30
AU2003274973A8 AU2003274973A8 (en) 2004-04-30

Family

ID=31994103

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003274973A Abandoned AU2003274973A1 (en) 2002-09-13 2003-09-15 Thermostable reverse transcriptases and uses thereof

Country Status (5)

Country Link
US (4) US20040209276A1 (https=)
EP (2) EP2325303B1 (https=)
JP (1) JP4634799B2 (https=)
AU (1) AU2003274973A1 (https=)
WO (1) WO2004024749A2 (https=)

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WO2007022045A2 (en) * 2005-08-10 2007-02-22 Stratagene California Mutant reverse transcriptase and methods of use
JP2011500087A (ja) * 2007-10-22 2011-01-06 ザ ウィスター インスティテュート テロメラーゼ活性を調節する化合物の同定方法
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KR101818126B1 (ko) * 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
US11465785B1 (en) * 2012-11-29 2022-10-11 David M. Dodgen Quick filling and self sealing fluid storage bag assembly
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US9353409B2 (en) * 2013-01-15 2016-05-31 Jun Euihum Lee Compositions and methods for RT-PCR
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JP6769869B2 (ja) * 2013-10-16 2020-10-14 ニユー・イングランド・バイオレイブス・インコーポレイテツド 強化された特性を有する逆転写酵素
JP2017503521A (ja) * 2014-01-22 2017-02-02 ライフ テクノロジーズ コーポレーション 高温核酸合成で使用するための新規な逆転写酵素
US11046940B2 (en) * 2016-04-12 2021-06-29 Solis Biodyne Ou Synthetic reverse transcriptases and uses thereof
WO2018057909A1 (en) 2016-09-23 2018-03-29 New England Biolabs, Inc. A mutant reverse transcriptase
EP4545649A3 (en) * 2016-11-11 2025-06-04 Bio-Rad Laboratories, Inc. Methods for processing nucleic acid samples
US11220677B2 (en) 2016-12-14 2022-01-11 Takara Bio Inc. Heat-resistant reverse transcriptase mutant
WO2018136404A1 (en) * 2017-01-19 2018-07-26 Asuragen, Inc. Methods of rna amplification
CN110382690B (zh) 2017-02-16 2024-05-24 生物辐射实验室股份有限公司 新型逆转录酶及其用途
US20180312822A1 (en) 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
WO2019177684A1 (en) * 2018-03-15 2019-09-19 Massachusetts Institute Of Technology Methods of quantifying rna and dna variants through sequencing employing phosphorothioates
EP3794113A4 (en) 2018-05-16 2022-03-23 Bio-Rad Laboratories, Inc. METHODS FOR PROCESSING NUCLEIC ACID SAMPLES
EP3805380B1 (en) * 2019-10-07 2025-12-03 Qiagen Beverly, LLC Improved thermostable viral reverse transcriptase
EP4073241A2 (en) 2019-12-11 2022-10-19 10X Genomics, Inc. Reverse transcriptase variants
US12435318B2 (en) 2020-07-20 2025-10-07 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
US12559734B2 (en) 2020-07-20 2026-02-24 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
CN113174381B (zh) * 2021-06-08 2023-05-12 翌圣生物科技(上海)股份有限公司 增强型mmlv逆转录酶突变体及其应用
EP4532747A1 (en) * 2022-06-01 2025-04-09 Bio-Rad Laboratories, Inc. Method and system for detecting reverse transcriptase activity by digital assay
WO2024064136A1 (en) * 2022-09-19 2024-03-28 Domus Diagnostics, Inc. Rapid molecular diagnostics with unified-one-pot sample processing, nucleic acid amplification, and result readout

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US5017492A (en) 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US4962022A (en) 1986-09-22 1990-10-09 Becton Dickinson And Company Storage and use of liposomes
US5244797B1 (en) 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5498523A (en) 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
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US7078208B2 (en) * 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
CN1430670A (zh) * 2000-05-26 2003-07-16 茵维特罗根公司 热稳定逆转录酶及其用途
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Also Published As

Publication number Publication date
US20070141592A1 (en) 2007-06-21
EP1554377B1 (en) 2012-11-07
JP4634799B2 (ja) 2011-02-16
EP1554377A2 (en) 2005-07-20
EP1554377A4 (en) 2008-03-19
EP2325303A2 (en) 2011-05-25
US20120021464A1 (en) 2012-01-26
JP2005538719A (ja) 2005-12-22
WO2004024749A3 (en) 2005-05-19
US20140363854A1 (en) 2014-12-11
US20040209276A1 (en) 2004-10-21
US9862938B2 (en) 2018-01-09
WO2004024749A2 (en) 2004-03-25
EP2325303B1 (en) 2013-11-06
EP2325303A3 (en) 2011-08-17
AU2003274973A8 (en) 2004-04-30
US8753845B2 (en) 2014-06-17

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase