JP2005537030A5 - - Google Patents
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- JP2005537030A5 JP2005537030A5 JP2004555256A JP2004555256A JP2005537030A5 JP 2005537030 A5 JP2005537030 A5 JP 2005537030A5 JP 2004555256 A JP2004555256 A JP 2004555256A JP 2004555256 A JP2004555256 A JP 2004555256A JP 2005537030 A5 JP2005537030 A5 JP 2005537030A5
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- Prior art keywords
- polynucleotide
- specific marker
- unit specific
- haplotype
- labeled
- Prior art date
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- 238000000034 method Methods 0.000 claims description 59
- 108091033319 polynucleotide Proteins 0.000 claims description 46
- 102000040430 polynucleotide Human genes 0.000 claims description 46
- 239000002157 polynucleotide Substances 0.000 claims description 46
- 239000003550 marker Substances 0.000 claims description 28
- 108091034117 Oligonucleotide Proteins 0.000 claims description 24
- 102000054766 genetic haplotypes Human genes 0.000 claims description 21
- 108020004414 DNA Proteins 0.000 claims description 19
- 239000002773 nucleotide Substances 0.000 claims description 18
- 238000004458 analytical method Methods 0.000 claims description 16
- 238000001514 detection method Methods 0.000 claims description 16
- 239000000523 sample Substances 0.000 claims description 16
- 102000039446 nucleic acids Human genes 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 15
- 150000007523 nucleic acids Chemical class 0.000 claims description 15
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- 238000009396 hybridization Methods 0.000 claims description 12
- 238000002372 labelling Methods 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
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- 230000000295 complement effect Effects 0.000 claims description 6
- -1 oligo beacon Proteins 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
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- 239000001226 triphosphate Substances 0.000 claims description 4
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- 238000009830 intercalation Methods 0.000 claims description 3
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- 108010010677 Phosphodiesterase I Proteins 0.000 claims 1
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- 229920000642 polymer Polymers 0.000 description 12
- 238000010586 diagram Methods 0.000 description 9
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 8
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- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
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- SAOKZLXYCUGLFA-UHFFFAOYSA-N bis(2-ethylhexyl) adipate Chemical compound CCCCC(CC)COC(=O)CCCCC(=O)OCC(CC)CCCC SAOKZLXYCUGLFA-UHFFFAOYSA-N 0.000 description 3
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- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
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- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
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- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
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- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
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- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000003505 Myosin Human genes 0.000 description 1
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- 102000005936 beta-Galactosidase Human genes 0.000 description 1
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- 229940116332 glucose oxidase Drugs 0.000 description 1
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- 238000003384 imaging method Methods 0.000 description 1
- 239000000138 intercalating agent Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 241000894007 species Species 0.000 description 1
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Images
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37946102P | 2002-05-09 | 2002-05-09 | |
| PCT/US2003/014776 WO2004048514A2 (en) | 2002-05-09 | 2003-05-09 | Methods for analyzing a nucleic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005537030A JP2005537030A (ja) | 2005-12-08 |
| JP2005537030A5 true JP2005537030A5 (enExample) | 2006-06-29 |
Family
ID=32393218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004555256A Withdrawn JP2005537030A (ja) | 2002-05-09 | 2003-05-09 | 核酸を分析する方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20030235854A1 (enExample) |
| EP (1) | EP1540017A4 (enExample) |
| JP (1) | JP2005537030A (enExample) |
| AU (1) | AU2003302463A1 (enExample) |
| WO (1) | WO2004048514A2 (enExample) |
Families Citing this family (85)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE273381T1 (de) | 1997-02-12 | 2004-08-15 | Eugene Y Chan | Verfahren zur analyse von polymeren |
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| US7282330B2 (en) | 2002-05-28 | 2007-10-16 | U.S. Genomics, Inc. | Methods and apparati using single polymer analysis |
| EP1546380A4 (en) * | 2002-05-28 | 2007-02-14 | Us Genomics Inc | METHODS AND APPARATUSES FOR ANALYZING SIMPLE POLYMERS |
| JP2007501003A (ja) * | 2003-08-01 | 2007-01-25 | ユー．エス． ジェノミクス， インコーポレイテッド | 非切断条件下で核酸を分析するための配列特異的エンドヌクレアーゼの使用に関連した方法および組成物 |
| WO2005017205A2 (en) * | 2003-08-04 | 2005-02-24 | U. S. Genomics, Inc. | Nucleic acid mapping using linear analysis |
| US20050042665A1 (en) * | 2003-08-21 | 2005-02-24 | U.S. Genomics, Inc. | Quantum dots and methods of use thereof |
| WO2005047470A2 (en) * | 2003-11-07 | 2005-05-26 | U.S. Genomics, Inc. | Intercalator fret donors or acceptors |
| US7977048B2 (en) | 2004-01-13 | 2011-07-12 | Pathogenetix, Inc. | Detection and quantification of analytes in solution using polymers |
| US7595160B2 (en) | 2004-01-13 | 2009-09-29 | U.S. Genomics, Inc. | Analyte detection using barcoded polymers |
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| WO2006017274A2 (en) * | 2004-07-13 | 2006-02-16 | U.S. Genomics, Inc. | Systems and methods for sample modification using fluidic chambers |
| US7351538B2 (en) | 2004-08-23 | 2008-04-01 | U.S. Genomics | Systems and methods for detecting and analyzing polymers |
| DE602005025782D1 (de) * | 2004-09-21 | 2011-02-17 | Life Technologies Corp | Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas) |
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| WO2007011660A2 (en) * | 2005-07-14 | 2007-01-25 | William Marsh Rice University | Quantum dot probes |
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| JP4767654B2 (ja) * | 2005-10-21 | 2011-09-07 | 株式会社エヌ・ティ・ティ・ドコモ | 分子伝送・分子配送システムおよび分子伝送・分子配送方法 |
| US20080241071A1 (en) * | 2006-07-14 | 2008-10-02 | West Jennifer L | Quantum Dot Probes |
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| JP2006520463A (ja) * | 2003-01-23 | 2006-09-07 | ユー．エス． ジェノミクス， インコーポレイテッド | ポリマー集団を解析するための方法 |
| JP2007501003A (ja) * | 2003-08-01 | 2007-01-25 | ユー．エス． ジェノミクス， インコーポレイテッド | 非切断条件下で核酸を分析するための配列特異的エンドヌクレアーゼの使用に関連した方法および組成物 |
| US20050042665A1 (en) * | 2003-08-21 | 2005-02-24 | U.S. Genomics, Inc. | Quantum dots and methods of use thereof |
| WO2005047470A2 (en) * | 2003-11-07 | 2005-05-26 | U.S. Genomics, Inc. | Intercalator fret donors or acceptors |
| US7977048B2 (en) * | 2004-01-13 | 2011-07-12 | Pathogenetix, Inc. | Detection and quantification of analytes in solution using polymers |
-
2003
- 2003-05-09 WO PCT/US2003/014776 patent/WO2004048514A2/en not_active Ceased
- 2003-05-09 AU AU2003302463A patent/AU2003302463A1/en not_active Abandoned
- 2003-05-09 US US10/435,399 patent/US20030235854A1/en not_active Abandoned
- 2003-05-09 JP JP2004555256A patent/JP2005537030A/ja not_active Withdrawn
- 2003-05-09 EP EP03811981A patent/EP1540017A4/en not_active Withdrawn
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