JP2005532058A5 - - Google Patents
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- Publication number
- JP2005532058A5 JP2005532058A5 JP2004518823A JP2004518823A JP2005532058A5 JP 2005532058 A5 JP2005532058 A5 JP 2005532058A5 JP 2004518823 A JP2004518823 A JP 2004518823A JP 2004518823 A JP2004518823 A JP 2004518823A JP 2005532058 A5 JP2005532058 A5 JP 2005532058A5
- Authority
- JP
- Japan
- Prior art keywords
- polynucleotide
- tag
- probe
- test kit
- degradable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims description 149
- 108091033319 polynucleotide Proteins 0.000 claims description 96
- 102000040430 polynucleotide Human genes 0.000 claims description 96
- 239000002157 polynucleotide Substances 0.000 claims description 96
- 239000000700 radioactive tracer Substances 0.000 claims description 38
- 239000012491 analyte Substances 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 34
- 238000012360 testing method Methods 0.000 claims description 25
- 238000009396 hybridization Methods 0.000 claims description 22
- 238000000926 separation method Methods 0.000 claims description 20
- 108020004414 DNA Proteins 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 12
- 239000002773 nucleotide Substances 0.000 claims description 12
- 125000003729 nucleotide group Chemical group 0.000 claims description 12
- 108091034117 Oligonucleotide Proteins 0.000 claims description 11
- 238000003149 assay kit Methods 0.000 claims description 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000005194 fractionation Methods 0.000 claims description 7
- 108020004999 messenger RNA Proteins 0.000 claims description 7
- 230000037230 mobility Effects 0.000 claims description 7
- 150000001413 amino acids Chemical class 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 6
- 238000001962 electrophoresis Methods 0.000 claims description 6
- 239000012634 fragment Substances 0.000 claims description 6
- 230000015556 catabolic process Effects 0.000 claims description 5
- 238000006731 degradation reaction Methods 0.000 claims description 5
- 230000000813 microbial effect Effects 0.000 claims description 5
- 108020004418 ribosomal RNA Proteins 0.000 claims description 5
- 238000004949 mass spectrometry Methods 0.000 claims description 4
- 238000004587 chromatography analysis Methods 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 238000011158 quantitative evaluation Methods 0.000 claims description 2
- 108091007491 NSP3 Papain-like protease domains Proteins 0.000 claims 2
- 238000010521 absorption reaction Methods 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 230000003115 biocidal effect Effects 0.000 claims 1
- 238000004020 luminiscence type Methods 0.000 claims 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 19
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 12
- 230000003321 amplification Effects 0.000 description 8
- 238000003199 nucleic acid amplification method Methods 0.000 description 8
- 229960002685 biotin Drugs 0.000 description 6
- 235000020958 biotin Nutrition 0.000 description 6
- 239000011616 biotin Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 102000004196 processed proteins & peptides Human genes 0.000 description 6
- 239000004170 rice bran wax Substances 0.000 description 6
- 241000193450 [Clostridium] symbiosum Species 0.000 description 5
- 238000003752 polymerase chain reaction Methods 0.000 description 5
- 241000193452 Clostridium tyrobutyricum Species 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000004445 quantitative analysis Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 241000499912 Trichoderma reesei Species 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000012733 comparative method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 108020005075 5S Ribosomal RNA Proteins 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 241000193171 Clostridium butyricum Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 241000186567 Romboutsia lituseburensis Species 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000005285 chemical preparation method Methods 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 108700022487 rRNA Genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI20021325A FI20021325A0 (fi) | 2002-07-05 | 2002-07-05 | Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi |
| PCT/FI2003/000544 WO2004005545A1 (en) | 2002-07-05 | 2003-07-04 | Method and test kit for quantitative determination of polynucleotides in a mixture |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005532058A JP2005532058A (ja) | 2005-10-27 |
| JP2005532058A5 true JP2005532058A5 (https=) | 2006-08-17 |
| JP4672365B2 JP4672365B2 (ja) | 2011-04-20 |
Family
ID=8564307
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004518823A Expired - Fee Related JP4672365B2 (ja) | 2002-07-05 | 2003-07-04 | 混合物中のポリヌクレオチドの定量のための方法および試験キット |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7361461B2 (https=) |
| EP (2) | EP1537238B1 (https=) |
| JP (1) | JP4672365B2 (https=) |
| AT (1) | ATE384141T1 (https=) |
| AU (1) | AU2003242804B2 (https=) |
| CA (1) | CA2489196C (https=) |
| DE (1) | DE60318723T2 (https=) |
| DK (1) | DK1537238T3 (https=) |
| ES (1) | ES2299727T3 (https=) |
| FI (1) | FI20021325A0 (https=) |
| PT (1) | PT1537238E (https=) |
| WO (1) | WO2004005545A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006090073A2 (fr) * | 2005-02-25 | 2006-08-31 | Comissariat A L'energie Atomique | Procede et dispositif pour separer des cibles moleculaires dans un melange complexe |
| EP1772522A1 (en) * | 2005-10-04 | 2007-04-11 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | Control of preservation by biomarkers |
| US20070190560A1 (en) * | 2006-02-13 | 2007-08-16 | Olga Ornatsky | Element-tagged olignucleotide gene expression analysis |
| EP2677039B8 (en) | 2006-05-10 | 2022-10-05 | DxTerity Diagnostics Incorporated | Detection of nucleic acid targets using chemically reactive oligonucleotide probes |
| US20090011944A1 (en) * | 2007-02-21 | 2009-01-08 | Valtion Teknillinen Tutkimuskekus | Method and test kit for detecting nucleotide variations |
| FI20075124A0 (fi) * | 2007-02-21 | 2007-02-21 | Valtion Teknillinen | Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi |
| US9976177B2 (en) | 2009-04-01 | 2018-05-22 | Dxterity Diagnostics Incorporated | Chemical ligation dependent probe amplification (CLPA) |
| US9745616B2 (en) | 2011-05-17 | 2017-08-29 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
| WO2013085631A1 (en) * | 2011-10-25 | 2013-06-13 | The Board Of Regents Of The University Of Texas System | Methods and compositions for identifying enterovirus |
| JP6695285B2 (ja) | 2014-06-10 | 2020-05-20 | ディクステリティー ダイアグノスティクス インコーポレイテッド | 生物学的試料を採取して安定化するためのデバイス及び方法 |
| CN109715827B (zh) | 2016-05-06 | 2023-01-10 | 明尼苏达大学董事会 | 分析标准品及其使用方法 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| NZ211453A (en) * | 1984-03-22 | 1989-01-06 | Biotechnology Research Enterpr | Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides |
| US4968602A (en) * | 1986-03-05 | 1990-11-06 | Molecular Diagnostics, Inc. | Solution-phase single hybridization assay for detecting polynucleotide sequences |
| AU619170B2 (en) * | 1987-01-09 | 1992-01-23 | Abbott Laboratories | Diagnostic assays using nucleic acid probes |
| US5082830A (en) * | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
| US5470705A (en) * | 1992-04-03 | 1995-11-28 | Applied Biosystems, Inc. | Probe composition containing a binding domain and polymer chain and methods of use |
| US5633134A (en) * | 1992-10-06 | 1997-05-27 | Ig Laboratories, Inc. | Method for simultaneously detecting multiple mutations in a DNA sample |
| US5605798A (en) * | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
| JP3102800B2 (ja) * | 1994-08-19 | 2000-10-23 | パーキン−エルマー コーポレイション | 増幅及び連結反応の共役法 |
| WO1996020212A2 (en) | 1994-12-28 | 1996-07-04 | Buchardt, Dorte | Peptide nucleic acid incorporating a chiral backbone |
| US6136531A (en) * | 1995-05-08 | 2000-10-24 | Roche Diagnostics Gmbh | Method of quantitatively detecting nucleic acids |
| DE19516196A1 (de) * | 1995-05-08 | 1996-11-14 | Boehringer Mannheim Gmbh | Verfahren zum quantitativen Nachweis von Nukleinsäuren |
| US5714386A (en) * | 1996-01-11 | 1998-02-03 | Board Of Trustees Of The Leland Stanford Junior University | Cy7-allophycocyanin conjugates for use in multiplex fluorescence detection assays |
| CN1202260C (zh) * | 1996-01-23 | 2005-05-18 | 佳根基因组学公司 | 利用大小分离技术分析核酸分子的方法和组合物 |
| EP1736554B1 (en) * | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| CN1251609A (zh) * | 1997-02-12 | 2000-04-26 | 尤金·Y·查恩 | 分析聚合物的方法和产品 |
| AU9063398A (en) | 1997-09-12 | 1999-04-05 | Exiqon A/S | Oligonucleotide analogues |
| US6562567B2 (en) | 1998-01-27 | 2003-05-13 | California Institute Of Technology | Method of detecting a nucleic acid |
| DE69940433D1 (de) * | 1998-08-11 | 2009-04-02 | Siemens Healthcare Diagnostics | Im nahen infrarot chemilumineszente akridinium derivate und ihre verwendung |
| US6480791B1 (en) * | 1998-10-28 | 2002-11-12 | Michael P. Strathmann | Parallel methods for genomic analysis |
| US6395486B1 (en) * | 1999-03-15 | 2002-05-28 | Applera Corporation | Probe/mobility modifier complexes for multiplexnucleic acid detection |
| FI115139B (fi) * | 2001-01-10 | 2005-03-15 | Valtion Teknillinen | Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen |
| WO2002097112A2 (en) * | 2001-05-26 | 2002-12-05 | Aclara Biosciences, Inc. | Catalytic amplification of multiplexed assay signals |
| WO2003033741A1 (en) * | 2001-10-16 | 2003-04-24 | Aclara Biosciences, Inc. | Universal e-tag primer and probe compositions and methods |
| AU2002351193A1 (en) * | 2001-11-28 | 2003-06-10 | Mj Bioworks Incorporated | Parallel polymorphism scoring by amplification and error correction |
| ATE414177T1 (de) * | 2002-09-30 | 2008-11-15 | Hoffmann La Roche | Oligonukleotide zur genotypisierung des thymidylat-synthase gens |
| US7354706B2 (en) * | 2003-09-09 | 2008-04-08 | The Regents Of The University Of Colorado, A Body Corporate | Use of photopolymerization for amplification and detection of a molecular recognition event |
| EP1694869A2 (en) * | 2003-11-10 | 2006-08-30 | Investigen, Inc. | Methods of preparing nucleic acid for detection |
-
2002
- 2002-07-05 FI FI20021325A patent/FI20021325A0/fi unknown
-
2003
- 2003-07-04 EP EP03762698A patent/EP1537238B1/en not_active Expired - Lifetime
- 2003-07-04 DE DE60318723T patent/DE60318723T2/de not_active Expired - Lifetime
- 2003-07-04 CA CA2489196A patent/CA2489196C/en not_active Expired - Fee Related
- 2003-07-04 US US10/519,403 patent/US7361461B2/en not_active Expired - Fee Related
- 2003-07-04 ES ES03762698T patent/ES2299727T3/es not_active Expired - Lifetime
- 2003-07-04 AT AT03762698T patent/ATE384141T1/de active
- 2003-07-04 JP JP2004518823A patent/JP4672365B2/ja not_active Expired - Fee Related
- 2003-07-04 DK DK03762698T patent/DK1537238T3/da active
- 2003-07-04 WO PCT/FI2003/000544 patent/WO2004005545A1/en not_active Ceased
- 2003-07-04 PT PT03762698T patent/PT1537238E/pt unknown
- 2003-07-04 AU AU2003242804A patent/AU2003242804B2/en not_active Ceased
- 2003-07-04 EP EP08100037A patent/EP1947200A1/en not_active Withdrawn
-
2008
- 2008-04-18 US US12/105,960 patent/US8143388B2/en not_active Expired - Fee Related
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