CA2489196C - Method and test kit for quantitative determination of polynucleotides in a mixture - Google Patents

Method and test kit for quantitative determination of polynucleotides in a mixture Download PDF

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Publication number
CA2489196C
CA2489196C CA2489196A CA2489196A CA2489196C CA 2489196 C CA2489196 C CA 2489196C CA 2489196 A CA2489196 A CA 2489196A CA 2489196 A CA2489196 A CA 2489196A CA 2489196 C CA2489196 C CA 2489196C
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CA
Canada
Prior art keywords
probes
molecules
affinity
tag
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2489196A
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English (en)
French (fr)
Other versions
CA2489196A1 (en
Inventor
Hans Soederlund
Reetta Satokari
Kari Kataja
Kristiina Takkinen
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VTT Technical Research Centre of Finland Ltd
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VTT Technical Research Centre of Finland Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by VTT Technical Research Centre of Finland Ltd filed Critical VTT Technical Research Centre of Finland Ltd
Publication of CA2489196A1 publication Critical patent/CA2489196A1/en
Application granted granted Critical
Publication of CA2489196C publication Critical patent/CA2489196C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2489196A 2002-07-05 2003-07-04 Method and test kit for quantitative determination of polynucleotides in a mixture Expired - Fee Related CA2489196C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI20021325 2002-07-05
FI20021325A FI20021325A0 (fi) 2002-07-05 2002-07-05 Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi
PCT/FI2003/000544 WO2004005545A1 (en) 2002-07-05 2003-07-04 Method and test kit for quantitative determination of polynucleotides in a mixture

Publications (2)

Publication Number Publication Date
CA2489196A1 CA2489196A1 (en) 2004-01-15
CA2489196C true CA2489196C (en) 2013-06-11

Family

ID=8564307

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2489196A Expired - Fee Related CA2489196C (en) 2002-07-05 2003-07-04 Method and test kit for quantitative determination of polynucleotides in a mixture

Country Status (12)

Country Link
US (2) US7361461B2 (https=)
EP (2) EP1947200A1 (https=)
JP (1) JP4672365B2 (https=)
AT (1) ATE384141T1 (https=)
AU (1) AU2003242804B2 (https=)
CA (1) CA2489196C (https=)
DE (1) DE60318723T2 (https=)
DK (1) DK1537238T3 (https=)
ES (1) ES2299727T3 (https=)
FI (1) FI20021325A0 (https=)
PT (1) PT1537238E (https=)
WO (1) WO2004005545A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2598508A1 (fr) * 2005-02-25 2006-08-31 Commissariat A L'energie Atomique Procede et dispositif pour separer des cibles moleculaires dans un melange complexe
EP1772522A1 (en) * 2005-10-04 2007-04-11 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Control of preservation by biomarkers
DK1991694T3 (da) 2006-02-13 2016-08-01 Fluidigm Canada Inc Genekspressionsanalyse med grundstoftagget oligonukleotid
CA2651815A1 (en) 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US20090011944A1 (en) * 2007-02-21 2009-01-08 Valtion Teknillinen Tutkimuskekus Method and test kit for detecting nucleotide variations
FI20075124A0 (fi) * 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
CA2757300C (en) * 2009-04-01 2018-01-09 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (clpa)
US9745616B2 (en) 2011-05-17 2017-08-29 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
WO2013085631A1 (en) * 2011-10-25 2013-06-13 The Board Of Regents Of The University Of Texas System Methods and compositions for identifying enterovirus
CN106879252B (zh) 2014-06-10 2020-07-17 德克斯特里蒂诊断公司 用于收集和稳定生物样品的装置及方法
CA3023248A1 (en) 2016-05-06 2017-11-09 Regents Of The University Of Minnesota Analytical standards and methods of using same

Family Cites Families (28)

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US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
NZ211453A (en) * 1984-03-22 1989-01-06 Biotechnology Research Enterpr Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides
US4968602A (en) * 1986-03-05 1990-11-06 Molecular Diagnostics, Inc. Solution-phase single hybridization assay for detecting polynucleotide sequences
AU619170B2 (en) * 1987-01-09 1992-01-23 Abbott Laboratories Diagnostic assays using nucleic acid probes
US5082830A (en) * 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
US5470705A (en) * 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
US5633134A (en) * 1992-10-06 1997-05-27 Ig Laboratories, Inc. Method for simultaneously detecting multiple mutations in a DNA sample
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
DE69528706T2 (de) * 1994-08-19 2003-06-12 Pe Corp. (Ny), Foster City Gekoppeltes ampflikation- und ligationverfahren
WO1996020212A2 (en) 1994-12-28 1996-07-04 Buchardt, Dorte Peptide nucleic acid incorporating a chiral backbone
DE19516196A1 (de) * 1995-05-08 1996-11-14 Boehringer Mannheim Gmbh Verfahren zum quantitativen Nachweis von Nukleinsäuren
US6136531A (en) 1995-05-08 2000-10-24 Roche Diagnostics Gmbh Method of quantitatively detecting nucleic acids
US5714386A (en) * 1996-01-11 1998-02-03 Board Of Trustees Of The Leland Stanford Junior University Cy7-allophycocyanin conjugates for use in multiplex fluorescence detection assays
DK0840804T3 (da) * 1996-01-23 2000-07-17 Rapigene Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyremolekyler ved anvendelse af størrelsesseparationsteknikker
EP2369007B1 (en) * 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
WO1998035012A2 (en) * 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
JP4236812B2 (ja) 1997-09-12 2009-03-11 エクシコン エ／エス オリゴヌクレオチド類似体
US6562567B2 (en) 1998-01-27 2003-05-13 California Institute Of Technology Method of detecting a nucleic acid
JP4672144B2 (ja) * 1998-08-11 2011-04-20 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッド 近赤外化学発光性アクリジニウム化合物およびその使用。
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
AU775179B2 (en) * 1999-03-15 2004-07-22 Applied Biosystems, Llc Probe/mobility modifier complexes for multiplex nucleic acid detection
FI115139B (fi) * 2001-01-10 2005-03-15 Valtion Teknillinen Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen
WO2002097112A2 (en) * 2001-05-26 2002-12-05 Aclara Biosciences, Inc. Catalytic amplification of multiplexed assay signals
WO2003033741A1 (en) * 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
DE60235491D1 (de) * 2001-11-28 2010-04-08 Bio Rad Laboratories Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
DE60229890D1 (de) * 2002-09-30 2008-12-24 Hoffmann La Roche Oligonukleotide zur genotypisierung des thymidylat-synthase gens
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
WO2005047521A2 (en) * 2003-11-10 2005-05-26 Investigen, Inc. Methods of preparing nucleic acid for detection

Also Published As

Publication number Publication date
FI20021325A0 (fi) 2002-07-05
JP2005532058A (ja) 2005-10-27
AU2003242804B2 (en) 2008-06-12
JP4672365B2 (ja) 2011-04-20
EP1537238B1 (en) 2008-01-16
EP1537238A1 (en) 2005-06-08
ATE384141T1 (de) 2008-02-15
DE60318723T2 (de) 2009-01-15
US7361461B2 (en) 2008-04-22
PT1537238E (pt) 2008-04-22
US20060035228A1 (en) 2006-02-16
AU2003242804A1 (en) 2004-01-23
DK1537238T3 (da) 2008-06-02
EP1947200A1 (en) 2008-07-23
DE60318723D1 (de) 2008-03-06
CA2489196A1 (en) 2004-01-15
US20080233591A1 (en) 2008-09-25
US8143388B2 (en) 2012-03-27
WO2004005545A1 (en) 2004-01-15
ES2299727T3 (es) 2008-06-01

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Effective date: 20220301

MKLA Lapsed

Effective date: 20200831