JP2005506525A5 - - Google Patents
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- JP2005506525A5 JP2005506525A5 JP2003517572A JP2003517572A JP2005506525A5 JP 2005506525 A5 JP2005506525 A5 JP 2005506525A5 JP 2003517572 A JP2003517572 A JP 2003517572A JP 2003517572 A JP2003517572 A JP 2003517572A JP 2005506525 A5 JP2005506525 A5 JP 2005506525A5
- Authority
- JP
- Japan
- Prior art keywords
- blood
- blood sample
- blood cells
- cells
- nucleated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 210000004369 Blood Anatomy 0.000 claims description 178
- 239000008280 blood Substances 0.000 claims description 178
- 210000003743 Erythrocytes Anatomy 0.000 claims description 91
- 210000003324 RBC Anatomy 0.000 claims description 87
- 210000000601 Blood Cells Anatomy 0.000 claims description 70
- 210000000265 Leukocytes Anatomy 0.000 claims description 70
- 239000000203 mixture Substances 0.000 claims description 57
- 239000003153 chemical reaction reagent Substances 0.000 claims description 53
- 210000002865 immune cell Anatomy 0.000 claims description 49
- 230000002934 lysing Effects 0.000 claims description 43
- 102000001554 Hemoglobins Human genes 0.000 claims description 39
- 108010054147 Hemoglobins Proteins 0.000 claims description 39
- 210000004027 cells Anatomy 0.000 claims description 32
- 230000009089 cytolysis Effects 0.000 claims description 29
- 238000002847 impedance measurement Methods 0.000 claims description 23
- 229940000351 Hemocyte Drugs 0.000 claims description 14
- 210000003677 Hemocytes Anatomy 0.000 claims description 14
- 239000011780 sodium chloride Substances 0.000 claims description 13
- 239000003085 diluting agent Substances 0.000 claims description 10
- 230000002452 interceptive Effects 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- 150000003839 salts Chemical class 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 6
- 238000002943 spectrophotometric absorbance Methods 0.000 claims description 5
- 238000004820 blood count Methods 0.000 claims description 2
- 238000007865 diluting Methods 0.000 claims 4
- 238000002835 absorbance Methods 0.000 claims 2
- 238000000034 method Methods 0.000 description 17
- 239000002245 particle Substances 0.000 description 16
- 238000005259 measurement Methods 0.000 description 14
- 230000002949 hemolytic Effects 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 8
- 210000004940 Nucleus Anatomy 0.000 description 7
- 238000000149 argon plasma sintering Methods 0.000 description 7
- 230000027455 binding Effects 0.000 description 6
- 239000003446 ligand Substances 0.000 description 6
- 230000004069 differentiation Effects 0.000 description 5
- 230000005684 electric field Effects 0.000 description 5
- CXRFDZFCGOPDTD-UHFFFAOYSA-M Cetrimide Chemical compound [Br-].CCCCCCCCCCCCCC[N+](C)(C)C CXRFDZFCGOPDTD-UHFFFAOYSA-M 0.000 description 4
- 125000000217 alkyl group Chemical group 0.000 description 4
- 125000004432 carbon atoms Chemical group C* 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000000684 flow cytometry Methods 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 239000004094 surface-active agent Substances 0.000 description 4
- 206010018910 Haemolysis Diseases 0.000 description 3
- 210000004698 Lymphocytes Anatomy 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 238000007430 reference method Methods 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 150000003536 tetrazoles Chemical class 0.000 description 3
- WPYMKLBDIGXBTP-UHFFFAOYSA-N Benzoic acid Chemical class OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 2
- 210000004494 Erythroblasts Anatomy 0.000 description 2
- RAXXELZNTBOGNW-UHFFFAOYSA-N Imidazole Chemical compound C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 2
- -1 alkaline earth metal salt Chemical class 0.000 description 2
- 125000003342 alkenyl group Chemical group 0.000 description 2
- 125000000304 alkynyl group Chemical group 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000002902 bimodal Effects 0.000 description 2
- 235000010354 butylated hydroxytoluene Nutrition 0.000 description 2
- XFXPMWWXUTWYJX-UHFFFAOYSA-N cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000003287 optical Effects 0.000 description 2
- 239000011236 particulate material Substances 0.000 description 2
- 150000003242 quaternary ammonium salts Chemical class 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- MCTWTZJPVLRJOU-UHFFFAOYSA-N 1-Methylimidazole Chemical compound CN1C=CN=C1 MCTWTZJPVLRJOU-UHFFFAOYSA-N 0.000 description 1
- PQAMFDRRWURCFQ-UHFFFAOYSA-N 2-ethyl-1H-imidazole Chemical compound CCC1=NC=CN1 PQAMFDRRWURCFQ-UHFFFAOYSA-N 0.000 description 1
- 208000007502 Anemia Diseases 0.000 description 1
- 210000003651 Basophils Anatomy 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- 241000219430 Betula pendula Species 0.000 description 1
- 210000001772 Blood Platelets Anatomy 0.000 description 1
- 210000001185 Bone Marrow Anatomy 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 210000000805 Cytoplasm Anatomy 0.000 description 1
- 210000003979 Eosinophils Anatomy 0.000 description 1
- 206010024324 Leukaemias Diseases 0.000 description 1
- 210000001616 Monocytes Anatomy 0.000 description 1
- 210000000440 Neutrophils Anatomy 0.000 description 1
- NNFCIKHAZHQZJG-UHFFFAOYSA-N Potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 150000003841 chloride salts Chemical class 0.000 description 1
- 150000001860 citric acid derivatives Chemical class 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N edta Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 159000000011 group IA salts Chemical class 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 150000002460 imidazoles Chemical class 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N oxane Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- LOAUVZALPPNFOQ-UHFFFAOYSA-N quinaldic acid Chemical compound C1=CC=CC2=NC(C(=O)O)=CC=C21 LOAUVZALPPNFOQ-UHFFFAOYSA-N 0.000 description 1
- 238000001374 small-angle light scattering Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- CUXKZYSCZCNPNX-UHFFFAOYSA-N tetradecan-1-amine;hydrobromide Chemical compound [Br-].CCCCCCCCCCCCCC[NH3+] CUXKZYSCZCNPNX-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/917,533 US6410330B1 (en) | 2001-07-27 | 2001-07-27 | Method for measurement of nucleated red blood cells |
| US16569902A | 2002-06-07 | 2002-06-07 | |
| PCT/US2002/023656 WO2003012429A1 (en) | 2001-07-27 | 2002-07-25 | Method for measurement of nucleated red blood cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005506525A JP2005506525A (ja) | 2005-03-03 |
| JP2005506525A5 true JP2005506525A5 (US06210969-20010403-C00002.png) | 2006-01-05 |
Family
ID=26861630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003517572A Pending JP2005506525A (ja) | 2001-07-27 | 2002-07-25 | 有核赤血球の計測法の方法 |
Country Status (5)
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6509192B1 (en) * | 1992-02-24 | 2003-01-21 | Coulter International Corp. | Quality control method |
| US6916658B2 (en) * | 2001-07-27 | 2005-07-12 | Beckman Coulter, Inc. | Method for measurement of immature granulocytes |
| US8986944B2 (en) * | 2001-10-11 | 2015-03-24 | Aviva Biosciences Corporation | Methods and compositions for separating rare cells from fluid samples |
| US8980568B2 (en) * | 2001-10-11 | 2015-03-17 | Aviva Biosciences Corporation | Methods and compositions for detecting non-hematopoietic cells from a blood sample |
| US7198953B2 (en) * | 2003-10-12 | 2007-04-03 | Beckman Coulter, Inc. | Method of using a reference control composition for measurement of nucleated red blood cells |
| US7195919B2 (en) * | 2003-12-19 | 2007-03-27 | Beckman Coulter, Inc. | Hematology controls for reticulocytes and nucleated red blood cells |
| US7008792B2 (en) | 2004-02-10 | 2006-03-07 | Beckman Coulter, Inc. | Method of measurement of nucleated red blood cells |
| JP4993603B2 (ja) * | 2004-04-07 | 2012-08-08 | ベックマン コールター， インコーポレイテッド | 有核赤血球成分を含む参照対照 |
| US7482165B2 (en) | 2005-08-24 | 2009-01-27 | Beckman Coulter, Inc. | Method of preventing white blood cell interferences to red blood cell measurements of a blood sample |
| US7354767B2 (en) * | 2006-03-16 | 2008-04-08 | Beckman Coulter, Inc. | Reference control composition containing a nucleated red blood cell component made of non-nucleated blood cells |
| KR100833793B1 (ko) * | 2006-05-16 | 2008-05-29 | 세원셀론텍(주) | 골수 유래 골 생성용 유핵 세포 분리 방법 |
| CN101583722A (zh) * | 2006-07-14 | 2009-11-18 | 阿维瓦生物科学股份有限公司 | 从生物学样品检测稀有细胞的方法和组合物 |
| US7754487B2 (en) * | 2006-11-14 | 2010-07-13 | Beckman Coulter, Inc. | Hematology linearity control composition, system and method of use |
| CN101723874B (zh) * | 2008-10-31 | 2013-09-11 | 深圳迈瑞生物医疗电子股份有限公司 | 花菁类化合物及其在生物样品染色中的用途 |
| WO2010056740A1 (en) * | 2008-11-13 | 2010-05-20 | Beckman Coulter, Inc. | Method of correction of particle interference to hemoglobin measurement |
| CN101750476B (zh) * | 2008-12-08 | 2015-06-03 | 深圳迈瑞生物医疗电子股份有限公司 | 血液分析试剂及其使用方法 |
| EP2216095A1 (en) * | 2009-01-27 | 2010-08-11 | Koninklijke Philips Electronics N.V. | Microfluidic device for full blood count |
| US8389293B2 (en) * | 2009-11-17 | 2013-03-05 | Abbott Point Of Care Inc. | Reducing leukocyte interference in competitive immunoassays |
| CN102109430B (zh) * | 2009-12-25 | 2013-11-06 | 深圳迈瑞生物医疗电子股份有限公司 | 有核红细胞模拟粒子，血液质控物及其制备方法和用途 |
| CN109946446A (zh) * | 2010-11-09 | 2019-06-28 | 综合医院公司 | 使用电差分计数器对颗粒计数 |
| US9096885B2 (en) * | 2012-07-05 | 2015-08-04 | Beckman Coulter, Inc. | Method and apparatus for determining white blood cell counts |
| JP6092132B2 (ja) * | 2014-01-29 | 2017-03-08 | シスメックス株式会社 | 血球分析装置 |
| EP3179249A4 (en) * | 2014-08-05 | 2017-07-26 | Fujifilm Corporation | Nucleated erythrocyte sorting method |
| WO2016106688A1 (zh) * | 2014-12-31 | 2016-07-07 | 深圳迈瑞生物医疗电子股份有限公司 | 一种有核红细胞报警方法、装置及流式细胞分析仪 |
| CN107817208A (zh) * | 2016-09-12 | 2018-03-20 | 深圳市帝迈生物技术有限公司 | 一种血细胞计数装置及其白细胞计数修正方法 |
| WO2019206312A1 (zh) | 2018-04-28 | 2019-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析仪异常的报警方法、系统及存储介质 |
| EP3789751A4 (en) | 2018-04-28 | 2021-06-30 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | METHOD AND SYSTEM FOR DETERMINING THROMBOCYTE CONCENTRATION |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2656508A (en) | 1949-08-27 | 1953-10-20 | Wallace H Coulter | Means for counting particles suspended in a fluid |
| US3810011A (en) | 1970-05-25 | 1974-05-07 | Coulter Electronics | Apparatus and method for analyzing the particle volume distribution for a plurality of particles of different size in a quantity of liquid |
| KR970007077B1 (ko) * | 1987-03-13 | 1997-05-02 | 코울터 일렉트로닉스 인커퍼레이티드 | 광산란 기술을 이용한 다중-부분식별 분석 방법 |
| US5242832A (en) | 1990-03-01 | 1993-09-07 | Toa Medical Electronics Co., Ltd. | Reagent for measurement of leukocytes and hemoglobin in blood |
| JP2927979B2 (ja) | 1991-02-22 | 1999-07-28 | シスメックス株式会社 | フローサイトメトリーによる赤芽球の分類方法 |
| US5376878A (en) * | 1991-12-12 | 1994-12-27 | Fisher; Timothy C. | Multiple-aperture particle counting sizing and deformability-measuring apparatus |
| AU6235994A (en) * | 1993-02-25 | 1994-09-14 | Abbott Laboratories | Multipurpose reagent system for rapid lysis of whole blood samples |
| AU1984295A (en) * | 1994-03-11 | 1995-09-25 | Abbott Laboratories | Cyanide-free reagent and method for the determination of hemoglobin |
| US5879900A (en) * | 1994-12-15 | 1999-03-09 | Abbott Laboratories | Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials |
| US5559037A (en) * | 1994-12-15 | 1996-09-24 | Abbott Laboratories | Method for rapid and simultaneous analysis of nucleated red blood cells |
| US5882933A (en) * | 1995-06-08 | 1999-03-16 | Coulter International Corp. | Method for determination of leukocytes and hemoglobin concentration in blood |
| US5817518A (en) * | 1995-12-18 | 1998-10-06 | Coulter International Corp. | Reagent and method for differential determination of leukocytes in blood |
| US5882934A (en) | 1997-01-21 | 1999-03-16 | Coulter International Corp. | Composition and method for hemoglobin and cell analysis |
| US5763280A (en) | 1997-01-21 | 1998-06-09 | Coulter International Corp. | Cyanide-free lytic reagent composition and method for hemoglobin and cell analysis |
| US5917584A (en) | 1997-11-21 | 1999-06-29 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
| US5874310A (en) * | 1997-11-21 | 1999-02-23 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
| JP3886271B2 (ja) | 1998-11-27 | 2007-02-28 | シスメックス株式会社 | 赤芽球の分類計数用試薬及び分類計数方法 |
| US6228652B1 (en) * | 1999-02-16 | 2001-05-08 | Coulter International Corp. | Method and apparatus for analyzing cells in a whole blood sample |
| US6472215B1 (en) * | 2001-07-27 | 2002-10-29 | Coulter International Corp. | Method of analyzing nucleated red blood cells in a blood sample |
| US6410330B1 (en) * | 2001-07-27 | 2002-06-25 | Coulter International Corp. | Method for measurement of nucleated red blood cells |
| US9502897B2 (en) | 2011-02-12 | 2016-11-22 | SolarBread LTD | Systems and methods for photovoltaic micro-inverter power harvesting efficiency increase in shaded conditions |
-
2002
- 2002-07-25 CN CN02814528.3A patent/CN1276252C/zh not_active Expired - Fee Related
- 2002-07-25 EP EP02752578.1A patent/EP1412740B1/en not_active Expired - Fee Related
- 2002-07-25 WO PCT/US2002/023656 patent/WO2003012429A1/en active Application Filing
- 2002-07-25 JP JP2003517572A patent/JP2005506525A/ja active Pending
- 2002-08-23 US US10/226,800 patent/US6673618B1/en not_active Expired - Lifetime
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