JP4268040B2 - 血液サンプル中の有核赤血球の分析法 - Google Patents
血液サンプル中の有核赤血球の分析法 Download PDFInfo
- Publication number
- JP4268040B2 JP4268040B2 JP2003517571A JP2003517571A JP4268040B2 JP 4268040 B2 JP4268040 B2 JP 4268040B2 JP 2003517571 A JP2003517571 A JP 2003517571A JP 2003517571 A JP2003517571 A JP 2003517571A JP 4268040 B2 JP4268040 B2 JP 4268040B2
- Authority
- JP
- Japan
- Prior art keywords
- blood cells
- sample
- red blood
- nucleated red
- blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000004369 blood Anatomy 0.000 title claims description 78
- 239000008280 blood Substances 0.000 title claims description 78
- 210000003743 erythrocyte Anatomy 0.000 title claims description 56
- 238000004458 analytical method Methods 0.000 title claims description 34
- 238000000034 method Methods 0.000 claims description 78
- 210000000265 leukocyte Anatomy 0.000 claims description 66
- 210000004027 cell Anatomy 0.000 claims description 56
- 210000000601 blood cell Anatomy 0.000 claims description 53
- 239000000203 mixture Substances 0.000 claims description 52
- 210000004698 lymphocyte Anatomy 0.000 claims description 49
- 238000005259 measurement Methods 0.000 claims description 43
- 239000003153 chemical reaction reagent Substances 0.000 claims description 36
- 238000002847 impedance measurement Methods 0.000 claims description 25
- 238000000149 argon plasma sintering Methods 0.000 claims description 21
- 238000012351 Integrated analysis Methods 0.000 claims description 13
- 206010018910 Haemolysis Diseases 0.000 claims description 12
- 230000008588 hemolysis Effects 0.000 claims description 12
- 238000003909 pattern recognition Methods 0.000 claims description 12
- 230000004069 differentiation Effects 0.000 claims description 8
- 230000002949 hemolytic effect Effects 0.000 claims description 8
- 238000000691 measurement method Methods 0.000 claims description 8
- 210000001616 monocyte Anatomy 0.000 claims description 6
- 239000003085 diluting agent Substances 0.000 claims description 5
- 210000003651 basophil Anatomy 0.000 claims description 4
- 210000003979 eosinophil Anatomy 0.000 claims description 4
- 210000000440 neutrophil Anatomy 0.000 claims description 4
- 210000003714 granulocyte Anatomy 0.000 claims description 3
- 239000003381 stabilizer Substances 0.000 claims description 2
- 230000006037 cell lysis Effects 0.000 claims 3
- 239000003219 hemolytic agent Substances 0.000 claims 1
- 239000000523 sample Substances 0.000 description 98
- 238000007405 data analysis Methods 0.000 description 20
- 230000009089 cytolysis Effects 0.000 description 8
- 210000003677 hemocyte Anatomy 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 229940000351 hemocyte Drugs 0.000 description 7
- 238000004820 blood count Methods 0.000 description 6
- 230000002159 abnormal effect Effects 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 230000002934 lysing effect Effects 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 210000000170 cell membrane Anatomy 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 238000000684 flow cytometry Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 210000003924 normoblast Anatomy 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 238000004159 blood analysis Methods 0.000 description 2
- 230000011748 cell maturation Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000569 multi-angle light scattering Methods 0.000 description 2
- 238000012758 nuclear staining Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 238000007430 reference method Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012105 stratification Analysis Methods 0.000 description 2
- 206010002065 Anaemia megaloblastic Diseases 0.000 description 1
- 208000000682 Megaloblastic Anemia Diseases 0.000 description 1
- 206010040642 Sickle cell anaemia with crisis Diseases 0.000 description 1
- 208000002903 Thalassemia Diseases 0.000 description 1
- 208000003441 Transfusion reaction Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- -1 aliphatic aldehyde Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000003271 compound fluorescence assay Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 231100001016 megaloblastic anemia Toxicity 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000001374 small-angle light scattering Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1019—Associating Coulter-counter and optical flow cytometer [OFC]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Ecology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
本発明は、血液サンプル中の有核赤血球を分析する方法に関する。前記方法は、以下のステップ:(a)血液サンプルの最初のアリコートを、最初の血球溶解試薬システムに晒して赤血球を溶解し、そして最初のサンプル混合物を形成し;(b)血液サンプルの2番目のアリコートを、2番目の血球溶解試薬システムに晒して赤血球を溶解し、そして2番目のサンプル混合物を形成し;(c)フローセル内の上記最初のサンプル混合物を、直流インピーダンス計測法(DC1)、無線周波インピーダンス計測法(RF)、及び光散乱計測法(LS)を含む検出によって計測し;(d)非絞り込みフロー・アパーチャ(non-focused flow aperture)における第2の直流インピーダンス測定法(DC2)によって上記2番目のサンプル混合物の血球分布を計測し;(e)上記最初のサンプル混合物を計測することで得られた血球分布パターンを分析し、そして有核赤血球を他の細胞型と区別し;(f)上記2番目のサンプル混合物を計測することで得られた血球分布パターンを分析し、そして有核赤血球を他の細胞型と区別し;(g)ステップ(e)及び(f)からの分析の結果を組み合わせることによって得られた特徴の複合解析を実施し、さらに上記複合解析が、有核赤血球を他の細胞型と区別し;そして(h)血液サンプル中の有核赤血球を記録する、を含む。前記方法は、血液サンプル中の有核赤血球の存在を記録することができて、さらに血液サンプル中の100個の白血球あたりの有核赤血球数として有核赤血球の量を記録することができる。
1の態様において、本発明は、血液サンプル中の有核赤血球(NRBCs)を分析するための方法に向けられる。
COULTER(登録商標)GEN・S(商標)血液学分析器により、EDTA-抗凝固処理した新鮮な正常な全血サンプルの最初のアリコートを、吸引し、混合チャンバー内で多量のErythrolyseIIと混ぜて赤血球を溶解させ、続いて大量のStabiLyseと混合して、この最初のサンプル混合物のさらなる溶解反応を遅らせる。前記最初のサンプル混合物を、シース流体、ISOTON(登録商標)III希釈液により絞り込みフローセルにデリバリーした。Erythrolyse(商標)II、StabiLyse(商標)、及びISOTON(登録商標)III希釈液は、Beckman Coulter, Inc. Miami, Floridaの製品である。
5 NRBCs/100 WBCを含む臨床全血サンプルを、実施例1に記載の同じ試薬及び手順を使って処理し、そして同じ条件下、COULTER(登録商標)GEN・S機器により計測した。NRBC濃度を、病院から提供された手作業によりカウントした100個の血球(cell)から得た。
13 NRBCs/100 WBCを含む臨床全血サンプルを、実施例1に記載されたのと同じ試薬及び手順を使用して処理し、そして同じ条件下、COULTER(登録商標)GEN・S機器により計測した。図3A及び3Bは、それぞれ、得られたDC1対RLS、及びDC1対不透過度の散布図である。図のように、第2のリンパ球の一団は、DC1において正常なリンパ球の下方に存在し、そしてNRBCsは、第2のリンパ球の一団の下方の領域に現われた。図3Cは、得られたDC2ヒストグラムである。従って、この場合、NRBC領域に及んだリンパ球集団は、DC2ヒストグラムにおけるリンパ球とNRBCsとの間の分離はなされなかった。
臨床全血サンプルを、実施例1に記載されたものと同じ試薬及び手順を使って処理し、そして同じ条件下、COULTER(登録商標)GEN・S機器により計測した。図4A及び4Bは、それぞれ、得られたDC1対RLS、及びDC1対不透過度の散布図である。図4Cは、得られたDC2ヒストグラムである。この血液サンプルについて、2番目のデータ分析はNRBCsの存在を同定しなかった。しかし、図4Aに示されるように、非常に低いDC1値をもった密度の高い細胞残屑の一団、及びDC1における小さな標準偏差が存在した。このタイプの細胞残屑分布特性は、通常、血液サンプル中のNRBCsの存在と関連しないことが分かり、それはNRBCsが幅広いサイズ分布をもつ傾向にあるという観察と一致している。統合分析は、この血液サンプルについてNRBCsは確認されなかった、そしてそれは手作業による基準の記録と一致していた。
Claims (17)
- 血液サンプル中の有核赤血球の分析方法であって、以下のステップ:
(a)血液サンプルの最初のアリコートを、最初の血球溶解試薬システムに晒して、赤血球を溶解させ、そして最初のサンプル混合物を形成し、
(b)血液サンプルの2番目のアリコートを、2番目の血球溶解試薬システムに晒して、赤血球を溶解させ、そして2番目のサンプル混合物を形成し、
(c)直流インピーダンス計測法(DC1)、無線周波インピーダンス計測法(RF)及び光散乱計測法(LS)によって上記最初のサンプル混合物を計測し、
(d)2番目の直流インピーダンス計測法(DC2)によって上記2番目のサンプル混合物を計測し、
(e)ステップ(c)における上記最初のサンプル混合物の計測から得られたデータに対して最初のパターン認識分析を実施して、有核赤血球の分析に関連する上記DC1、RF、及びLS計測における血液細胞分布パターンの多変数を作成し、そして上記有核赤血球の存在を確認するか又は他の細胞型と有核赤血球とを区別し、
(f)ステップ(d)における上記2番目のサンプル混合物の計測から得られたデータに対して2番目パターン認識分析を実施して、有核赤血球の分析に関連する上記DC2計測における血液細胞分布パターンの多変数を作成し、そして上記有核赤血球の存在を確認するか又は他の細胞型と有核赤血球とを区別し、
(g)ステップ(e)及び(f)において実施された上記最初及び2番目パターン認識の結果を組み合わせることによって得られる特徴の統合分析を実施し、ここで、当該統合分析は上記有核赤血球を計数し、そして
(h)上記血液サンプル中の上記有核赤血球の濃度を記録する、
を含む前記分析方法。 - 前記の有核赤血球の濃度を記録するステップが、前記血液サンプル中の、白血球100個あたりの有核赤血球の数を記録することを含む、請求項1に記載の方法。
- 前記の2番目の直流インピーダンス計測法(DC2)によって前記2番目のサンプル混合物を計測するステップが、非絞り込みフロー・アパーチャによって実施される、請求項1に記載の方法。
- 3番目の直流インピーダンス計測法によって前記非絞り込みフロー・アパーチャを用いて前記2番目のサンプル混合物中に残存している血球をカウントし、そして前記血液サンプルの単位体積あたりの白血球の数を記録することをさらに含む、請求項3に記載の方法。
- 前記有核赤血球量の濃度を記録するステップが、前記血液サンプルの単位体積中の有核赤血球の数を記録することをさらに含む、請求項4に記載の方法。
- 前記2番目の直流インピーダンス計測法(DC2)によって前記2番目のサンプル混合物を計測するステップと、前記3番目の直流インピーダンス計測法によって前記2番目のサンプル混合物中に残存している血球をカウントするステップとが、前記非絞り込みフロー・アパーチャによって同時に実施される、請求項5に記載の方法。
- 前記最初のサンプル混合物の、直流インピーダンス計測法(DC1)、無線周波インピーダンス計測法(RF)、及び光散乱計測法(LS)が、同時に実施される、請求項1に記載の方法。
- 前記光散乱計測法が、光散乱シグナルのメジアン角を使って実施される、請求項7に記載の方法。
- 前記光散乱シグナルのメジアン角が、約10°〜約70°の範囲内にある、請求項8に記載の方法。
- 前記の最初のパターン認識分析が、前記計測値と当該計測値の導関数の各々、2つの上記計測値と当該計測値の導関数の複数の組み合わせ、及び3つの上記計測値と当該計測値の導関数の少なくとも1つの組み合わせから得られる血球分布パターンを分析するステップを含む、請求項1に記載の方法。
- 前記計測値と当該計測値の導関数が、DC1、RF、不透過度(RFとDC1の関数)、LS、RLS(最初の変換光散乱)、及びFLS(2番目の変換光散乱)を含む、請求項10に記載の方法。
- 前記の2つの前記計測値と当該計測値の導関数の組み合わせが、DC1対不透過度、DC1対RLS、及び不透過度対RLSを含む、請求項10に記載の方法。
- 前記の3つの前記計測値と当該計測値の導関数の少なくとも1つの組み合わせが、DC1、不透過度、及びRLSの組み合わせ、並びにDC1、不透過度及びFLSの組み合わせを含む、請求項10に記載の方法。
- 前記最初のパターン認識分析が、リンパ球、単球、好中球、好酸球及び好塩基球を含む5つの白血球亜集団の区別をさらに含む、請求項10に記載の方法。
- 前記2番目のパターン認識分析が、リンパ球、単球及び顆粒球を含む3つの白血球亜集団の区別を含む、請求項1に記載の方法。
- 前記最初の血球溶解試薬システムが、血球溶解剤、及び安定化剤を含む、請求項1に記載の方法。
- 前記第2の血球溶解試薬システムが、血液希釈液、及び血球溶解試薬を含む、請求項1に記載の方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/917,450 US6472215B1 (en) | 2001-07-27 | 2001-07-27 | Method of analyzing nucleated red blood cells in a blood sample |
PCT/US2002/022416 WO2003012428A1 (en) | 2001-07-27 | 2002-07-15 | Method of analyzing nucleated red blood cells in a blood sample |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2004537727A JP2004537727A (ja) | 2004-12-16 |
JP2004537727A5 JP2004537727A5 (ja) | 2006-01-05 |
JP4268040B2 true JP4268040B2 (ja) | 2009-05-27 |
Family
ID=25438797
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2003517571A Expired - Lifetime JP4268040B2 (ja) | 2001-07-27 | 2002-07-15 | 血液サンプル中の有核赤血球の分析法 |
Country Status (4)
Country | Link |
---|---|
US (1) | US6472215B1 (ja) |
EP (1) | EP1417481B1 (ja) |
JP (1) | JP4268040B2 (ja) |
WO (1) | WO2003012428A1 (ja) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6916658B2 (en) * | 2001-07-27 | 2005-07-12 | Beckman Coulter, Inc. | Method for measurement of immature granulocytes |
EP1412740B1 (en) * | 2001-07-27 | 2015-07-08 | Beckman Coulter, Inc. | Method for measurement of nucleated red blood cells |
US6723563B2 (en) * | 2001-12-03 | 2004-04-20 | Streck Laboratories Inc. | Hematology reference control |
US6653137B2 (en) * | 2001-12-03 | 2003-11-25 | Streck Laboratories Inc. | Hematology reference control |
US7198953B2 (en) * | 2003-10-12 | 2007-04-03 | Beckman Coulter, Inc. | Method of using a reference control composition for measurement of nucleated red blood cells |
WO2005043113A2 (en) * | 2003-10-12 | 2005-05-12 | Beckman Coulter, Inc. | Method of using a reference control composition for measurement of nucleated red blood cells |
US7195919B2 (en) * | 2003-12-19 | 2007-03-27 | Beckman Coulter, Inc. | Hematology controls for reticulocytes and nucleated red blood cells |
US7208319B2 (en) * | 2004-02-10 | 2007-04-24 | Beckman Coulter, Inc. | Method of measurement of nucleated red blood cells |
JP4911601B2 (ja) * | 2004-02-10 | 2012-04-04 | ベックマン コールター, インコーポレイテッド | 有核赤血球細胞の計測方法 |
WO2005100979A1 (en) * | 2004-04-07 | 2005-10-27 | Beckman Coulter, Inc. | Reference control containing a nucleated red blood cell component |
JP2006313151A (ja) * | 2005-04-07 | 2006-11-16 | Sysmex Corp | 血液分析装置、試料分析装置及びフローサイトメータ |
US7482165B2 (en) * | 2005-08-24 | 2009-01-27 | Beckman Coulter, Inc. | Method of preventing white blood cell interferences to red blood cell measurements of a blood sample |
US7354767B2 (en) * | 2006-03-16 | 2008-04-08 | Beckman Coulter, Inc. | Reference control composition containing a nucleated red blood cell component made of non-nucleated blood cells |
EP2082224B1 (en) | 2006-11-14 | 2015-05-13 | Beckman Coulter, Inc. | Hematology linearity control composition system and method of use |
US7674622B2 (en) * | 2006-12-22 | 2010-03-09 | Abbott Laboratories, Inc. | Method for determination of nucleated red blood cells and leukocytes in a whole blood sample in an automated hematology analyzer |
JP4817450B2 (ja) * | 2007-01-31 | 2011-11-16 | シスメックス株式会社 | 血液分析装置、血液分析方法およびコンピュータプログラム |
AP2937A (en) * | 2007-04-20 | 2014-07-31 | Gen Hospital Corp | Method for counting cells |
WO2010012002A1 (en) * | 2008-07-25 | 2010-01-28 | Saryna Medical Corporation | Methods and systems for genetic analysis of fetal nucleated red blood cells |
US8512977B2 (en) * | 2008-09-19 | 2013-08-20 | Beckman Coulter, Inc. | Analyzing reticulocytes |
US8603773B2 (en) | 2008-09-19 | 2013-12-10 | Beckman Coulter | Method and system for analyzing a blood sample |
CN101723874B (zh) * | 2008-10-31 | 2013-09-11 | 深圳迈瑞生物医疗电子股份有限公司 | 花菁类化合物及其在生物样品染色中的用途 |
CN101750476B (zh) * | 2008-12-08 | 2015-06-03 | 深圳迈瑞生物医疗电子股份有限公司 | 血液分析试剂及其使用方法 |
US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
CA2970734C (en) * | 2013-12-17 | 2019-09-24 | Alentic Microscience Inc. | Dosimeters including lensless imaging systems |
CN116609243A (zh) * | 2018-04-28 | 2023-08-18 | 深圳迈瑞生物医疗电子股份有限公司 | 血液分析方法、血液分析系统及存储介质 |
CN111812012B (zh) * | 2020-06-29 | 2023-08-11 | 迈克医疗电子有限公司 | 有核红细胞区域的识别方法、装置及血液分析仪 |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2656508A (en) | 1949-08-27 | 1953-10-20 | Wallace H Coulter | Means for counting particles suspended in a fluid |
US4962038A (en) | 1980-06-16 | 1990-10-09 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
US4528274A (en) | 1982-07-06 | 1985-07-09 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
KR970007077B1 (ko) | 1987-03-13 | 1997-05-02 | 코울터 일렉트로닉스 인커퍼레이티드 | 광산란 기술을 이용한 다중-부분식별 분석 방법 |
US5155044A (en) | 1987-03-13 | 1992-10-13 | Coulter Electronics, Inc. | Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples |
JP2935529B2 (ja) * | 1990-03-01 | 1999-08-16 | シスメックス株式会社 | 白血球分類方法および試薬 |
AU613642B2 (en) * | 1987-05-29 | 1991-08-08 | Toa Medical Electronics Co., Ltd. | Method for classifying leukocytes and reagents |
US5040112A (en) | 1988-12-07 | 1991-08-13 | Serono-Baker Diagnostics, Inc. | Method of separating the three major types of blood cells from a white blood cell histogram |
JP2927979B2 (ja) | 1991-02-22 | 1999-07-28 | シスメックス株式会社 | フローサイトメトリーによる赤芽球の分類方法 |
JP3048260B2 (ja) * | 1991-07-29 | 2000-06-05 | シスメックス株式会社 | 白血球分類計数用試料調製方法 |
DE69434942T2 (de) * | 1993-02-25 | 2007-11-29 | Abbott Laboratories, Abbott Park | Mehrzweckreagenzsystem zur schnellen lysierung von vollblutproben |
AU6830894A (en) * | 1993-05-14 | 1994-12-12 | Coulter Corporation | Reticulocyte analyzing method and apparatus utilizing light scatter techniques |
US5559037A (en) | 1994-12-15 | 1996-09-24 | Abbott Laboratories | Method for rapid and simultaneous analysis of nucleated red blood cells |
US5879900A (en) | 1994-12-15 | 1999-03-09 | Abbott Laboratories | Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials |
US5834315A (en) | 1994-12-23 | 1998-11-10 | Coulter Corporation | Cyanide-free reagent and method for hemoglobin determination and leukocyte differentitation |
US5686308A (en) | 1995-06-08 | 1997-11-11 | Coulter Corporation | Reagent and method for differential determination of leukocytes in blood |
US5817518A (en) * | 1995-12-18 | 1998-10-06 | Coulter International Corp. | Reagent and method for differential determination of leukocytes in blood |
US5935857A (en) | 1997-08-01 | 1999-08-10 | Coulter International Corp. | Blood diluent |
US5917584A (en) | 1997-11-21 | 1999-06-29 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
US5874310A (en) | 1997-11-21 | 1999-02-23 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
JP3886271B2 (ja) | 1998-11-27 | 2007-02-28 | シスメックス株式会社 | 赤芽球の分類計数用試薬及び分類計数方法 |
US6228652B1 (en) * | 1999-02-16 | 2001-05-08 | Coulter International Corp. | Method and apparatus for analyzing cells in a whole blood sample |
US6410330B1 (en) * | 2001-07-27 | 2002-06-25 | Coulter International Corp. | Method for measurement of nucleated red blood cells |
-
2001
- 2001-07-27 US US09/917,450 patent/US6472215B1/en not_active Expired - Lifetime
-
2002
- 2002-07-15 WO PCT/US2002/022416 patent/WO2003012428A1/en active Application Filing
- 2002-07-15 EP EP02748164.7A patent/EP1417481B1/en not_active Expired - Lifetime
- 2002-07-15 JP JP2003517571A patent/JP4268040B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US6472215B1 (en) | 2002-10-29 |
EP1417481B1 (en) | 2017-12-20 |
WO2003012428A1 (en) | 2003-02-13 |
JP2004537727A (ja) | 2004-12-16 |
EP1417481A4 (en) | 2005-07-13 |
EP1417481A1 (en) | 2004-05-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4268040B2 (ja) | 血液サンプル中の有核赤血球の分析法 | |
US10859484B2 (en) | Method for determining volume and hemoglobin content of individual red blood cells | |
JP2004537727A5 (ja) | ||
JP4366478B2 (ja) | 赤芽球の識別方法 | |
JP5230648B2 (ja) | 自動血液分析装置による全血試料中の有核赤血球及び白血球細胞の測定方法 | |
EP1049919B1 (en) | Method for differentiation of nucleated red blood cells | |
JP3048260B2 (ja) | 白血球分類計数用試料調製方法 | |
EP1412740B1 (en) | Method for measurement of nucleated red blood cells | |
US6410330B1 (en) | Method for measurement of nucleated red blood cells | |
JP2005506525A5 (ja) | ||
US20180136109A1 (en) | Method for Hematology Analysis | |
JP4087560B2 (ja) | 赤芽球の識別方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20050714 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20050714 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20070607 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20070612 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20070911 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20070919 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20071116 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20080304 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20080603 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20080610 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080818 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20090120 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20090219 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 4268040 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120227 Year of fee payment: 3 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130227 Year of fee payment: 4 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130227 Year of fee payment: 4 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140227 Year of fee payment: 5 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
EXPY | Cancellation because of completion of term |