JP2005179278A - Fat discharge promotor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a fat discharge promotor containing a heretofore unknown tea constituent as an active ingredient. <P>SOLUTION: This fat discharge accelerator is a quite novel fat discharge accelerator containing a tea-derived polysaccharide as an active ingredient. This agent is considered to be effective to prevent obesity by inhibiting the accumulation of fats in the body by inhibiting the absorption of excessive fats derived from meals and discharging them from the body. The accelerator is made from a heretofore eaten material of a natural origin, and is therefore highly safe to humans, and can be administered for a long term without any risk of side effects. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a lipid excretion enhancer containing a polysaccharide derived from tea as an active ingredient and an oral application target comprising an effective amount of the lipid excretion enhancer.

In recent years, while eating habits have been enriched in Japan, the intake of high-fat foods or high-cholesterol foods, which are typical Western foods, has increased. Lipids such as cholesterol and neutral fat are necessary ingredients to make the body, but if the intake of these lipids increases too much, excess lipid accumulates in the body and eventually causes obesity. Obesity is a major problem because it triggers various so-called adult diseases. Therefore, if unnecessary lipid remaining in the body can be discharged out of the body, it becomes difficult for the lipid to accumulate in the body, and as a result, obesity can be prevented.
Against this background, in the food industry, various functional foods are being developed from the viewpoint of preventive medicine. In particular, it is desirable that naturally occurring materials used for edible foods promote the discharge of unnecessary lipids to the outside of the body and prevent obesity, because it is highly safe for the human body and does not need to worry about side effects.
On the other hand, tea has been attracting attention because it has various excellent pharmacological actions. For example, tea catechin, which is a representative of its components, is known to have an action of promoting lipid excretion into feces. (See Patent Document 1 and Non-Patent Document 1 below).
However, the elucidation of the action of tea components has not been sufficiently performed, and there are many unknown parts. Therefore, the search for the action of a tea ingredient that has not been known so far is very significant.
Japanese Patent No. 2812682 J. et al. Nutr. Sci. Vitaminol, Vol. 32, 1986, p. 613-622

  Therefore, an object of the present invention is to provide a lipid excretion enhancer comprising a tea ingredient that has not been known so far as an active ingredient.

  As a result of various studies in view of the above points, the present inventors have found that a polysaccharide derived from tea has an excellent action of promoting lipid excretion into feces. The tea-derived polysaccharide having a pharmacological action has a molecular weight of about 40,000, and the tea-derived polysaccharide mainly composed of L-arabinose, D-ribose, and D-glucose has a hypoglycemic action. A hypoglycemic agent, an antidiabetic agent and a health food containing the polysaccharide as an active ingredient have been proposed (Japanese Patent Laid-Open No. 63-308001). Furthermore, an antihyperlipidemic agent containing this polysaccharide as an active ingredient has been proposed (Japanese Patent Laid-Open No. 2-286620). In addition, a plasma cholesterol-lowering agent has been proposed comprising a polysaccharide obtained by extracting tea leaves with water or warm water, in particular, a polysaccharide having a molecular weight of about 20,000 to about 200,000 (Japanese Patent Laid-Open No. 6-80580). Issue gazette). However, there is no example reported so far on the lipid excretion promoting action of tea-derived polysaccharides, and it is surprising that tea-derived polysaccharides have such actions.

The present invention has been made based on the above findings, and the lipid excretion enhancer of the present invention is characterized in that, as described in claim 1, a tea-derived polysaccharide is an active ingredient.
The lipid excretion enhancer according to claim 2 is characterized in that, in the lipid excretion enhancer according to claim 1, the polysaccharide has a molecular weight of 400,000 or more.
Moreover, the oral application object of this invention is characterized by mix | blending the effective amount of the lipid excretion promoter of Claim 1 or 2 as described in Claim 3.

  The lipid excretion enhancer of the present invention is a completely new lipid excretion enhancer comprising a polysaccharide derived from tea as an active ingredient. Thus, it is considered that by suppressing the absorption of excess lipid derived from meals and excreting it from the body, the increase in lipids in the body is suppressed and it is effective in preventing obesity. The lipid excretion enhancer of the present invention is naturally derived from raw materials that have been conventionally used for food, so it is highly safe for the human body and can be taken continuously for a long time without having to worry about side effects. it can.

  The tea-derived polysaccharide (tea polysaccharide) in the present invention may be a water-soluble polysaccharide or an insoluble polysaccharide as long as it is derived from tea, and is not particularly limited. In general, a polysaccharide is a saccharide other than a monosaccharide or oligosaccharide, and refers to a polymer having a polymerization degree of 10 or more.

  The molecular weight of the tea polysaccharide used as an active ingredient of the lipid excretion enhancer in the present invention is not particularly limited, and can exert a lipid excretion promoting effect at any molecular weight. Those having a molecular weight of 400,000 or more are preferable because they exhibit an excellent lipid excretion promoting action. Tea polysaccharides having a desired molecular weight can be easily obtained by appropriately selecting a filtration membrane. Since the upper limit of the molecular weight is not particularly limited, any size polysaccharide may be used, but it is preferably one that can pass through a microfiltration membrane having a membrane pore size of 1.0 μm. When a component that cannot pass through a microfiltration membrane having a membrane pore diameter of 1.0 μm is included, it is not preferable because a certain effect or more cannot be obtained.

  In the present invention, the constituent sugars of the tea polysaccharide used as an active ingredient of the lipid excretion enhancer are not particularly limited, but preferably contain at least galacturonic acid, galactose and arabinose as constituent sugars. Further, the ratio of the constituent sugar contained in the tea polysaccharide is not particularly limited, but preferably the total constituent ratio of galacturonic acid, galactose and arabinose is 15% by weight or more of the total constituent sugar in the tea polysaccharide, More preferably, it is 30% by weight or more. In addition, the component ratio of each component of galacturonic acid, galactose, and arabinose is not particularly limited.

  Below, the manufacturing method of the lipid elimination promoter of this invention is demonstrated in detail.

  The tea in the present invention means any of leaves, stems, xylem, roots and fruits obtained from a tea tree (Camellia sinensis) belonging to the camellia family, or a mixture of two or more of these, raw or dried products. It does n’t matter. Moreover, it may be any one of ready-made black tea, fermented tea such as puer tea, semi-fermented tea such as oolong tea and baked tea, non-fermented tea such as green tea, pot-roasted green tea, and roasted tea. It may be a mixture of more than one type.

  Tea polysaccharides can be obtained, for example, from tea extracts. The tea extract can be prepared by subjecting the tea as it is or after pulverization to the extraction operation, or after drying and pulverizing as necessary, for the extraction operation. The dried pulverized product can be prepared by drying the tea leaves, stems, xylem, roots and fruits as they are and then pulverizing them, or cutting them raw and then drying them.

  As the extraction method, a generally used method can be adopted, and is not particularly limited. For example, tea (as it is or coarsely-sliced or chopped product) in water, hot water or hot water, or a dry pulverized product thereof (powder) Etc.), a method of performing extraction while heating and stirring, and the like. The obtained extract may be used as it is after removing solids by filtration or centrifugation as necessary, and may be used as it is, or it may be further concentrated, or it may be freeze-dried or spray-dried. May be used after being powdered. Furthermore, you may remove and use components other than tea polysaccharides, such as catechins. The tea extract as used in the present invention is not limited to those prepared by the extraction operation as described above, and any tea extract may be used as long as it contains tea-derived polysaccharides. In addition to the one prepared by the extraction operation as described above, for example, the tea before being subjected to the extraction operation may be used as it is, or the tea leaves that are the extraction residue may be used. In general, commercially available tea extracts may be used. Examples of such commercially available products include Tokyo Food Techno Co., Ltd. “Polyphenone”, Taiyo Kagaku Co., Ltd. “Sunphenon”, and ITO EN Co., Ltd. And the like.

  To prepare a tea polysaccharide from a tea extract, for example, a water-containing organic solvent such as 50-90% ethanol aqueous solution or an organic solvent such as ethanol is added to the tea extract and stirred well. Collect by centrifugation. Then, a water-containing organic solvent such as the above ethanol aqueous solution or an organic solvent such as ethanol is added to the insoluble matter again, and after sufficient stirring, the insoluble matter is collected using centrifugation or the like. After repeating this operation several times, if the collected insoluble matter is dried by a method such as vacuum drying, freeze drying, spray drying or the like, crude tea polysaccharides can be obtained.

  In order to purify the tea polysaccharide from the crude tea polysaccharide, first, water or the like is added to the obtained crude tea polysaccharide and stirred well to obtain a suspension. A method of separating the suspension according to the molecular weight, such as microfiltration, ultrafiltration, or gel filtration, is adopted, and a suitable filtration membrane or gel filtration material is selected according to the desired molecular weight. This can be done by fractionating. For example, in order to purify a tea polysaccharide having a molecular weight of 400,000 or more, the suspension may be permeated through an ultrafiltration membrane having a fractional molecular weight of 400,000. For the desired molecular weight tea polysaccharide, first, a desired molecular weight fraction is obtained from the tea extract, and a water-containing organic solvent such as 50-90% ethanol aqueous solution or an organic solvent such as ethanol is added to this fraction. You may make it obtain.

  The components once fractionated can be further finely separated and purified by changing the size of the filtration membrane or gel filtration material. For example, it is possible to fractionate more than 2 million components from components having a molecular weight of 400,000 or more, or fractionate components that can pass through a microfiltration membrane having a membrane pore diameter of 1.0 μm and have a molecular weight of 400,000 or more. Is possible.

  As described above, the tea polysaccharide purified from the crude tea polysaccharide can be obtained by appropriately selecting a filtration membrane or gel filtration material in accordance with the size of the tea polysaccharide to be obtained.

  Furthermore, after suspending the crude tea polysaccharide in water, warm water or hot water, the precipitate is collected by centrifugation, filtration, etc., and the collected precipitate is washed by repeating the above operation, Insoluble tea polysaccharides can be purified from the crude tea polysaccharides. Water-soluble tea polysaccharides can be recovered from the water-soluble fraction obtained by the above operation. Further, for example, it is possible to fractionate the crude tea polysaccharide in advance into a tea polysaccharide fraction having a molecular weight of 400,000 or more, and then collect the insoluble polysaccharide by centrifugation or filtration. The insoluble tea polysaccharide and water-soluble tea polysaccharide obtained in this way may be used alone or in a mixture at an appropriate ratio.

  The tea polysaccharide obtained as described above can be dried and powdered by a method such as freeze drying or spray drying, if necessary. Moreover, as another method, ethanol can be added to these so that it may become 50-90%, a tea polysaccharide can be precipitated, and it can also be made into powder by drying after collect | recovering deposits.

  Since the lipid excretion enhancer prepared by the method as described above is a component obtained from tea that is drunk daily in large quantities, it is highly safe and can be mass-produced. Further, the production can be performed relatively easily.

  The lipid excretion enhancer of the present invention may be a mixture of tea polysaccharides obtained by the above method as it is, or the tea polysaccharides may be used using ion exchange resins, adsorption resins, gel filtration materials, etc. Individual tea polysaccharides obtained by further fine purification may be used. Further, two or more kinds of tea polysaccharides obtained by purification may be used in combination at a desired mixing ratio. Whichever method is used, the lipid excretion promoting agent of the present invention can exert the effect of promoting the excretion of lipid into feces. The lipid herein may be anything that is generally interpreted, and means at least one selected from total cholesterol, neutral fat, phospholipid, free fatty acid, or total lipid summing up these. Can be interpreted.

  The lipid excretion promoting agent of the present invention exhibits an excellent lipid excretion promoting effect by blending an effective amount in an oral application target. In the present invention, the oral application target may be anything as long as it can be contained in the mouth, but is preferably a food / drink, a pharmaceutical, a quasi-drug, or the like that allows the tea polysaccharide to reach the intestine. Can be exemplified.

  The object to be applied to the oral cavity of the present invention may be in any form as long as it can be blended with tea polysaccharides as an active ingredient of a lipid excretion enhancer, for example, an aqueous solution, a turbid liquid or an emulsion. It may be a liquid form such as a gel, a semi-solid form such as a paste, or a solid form such as a powder, granule, capsule or tablet.

  Among the oral application objects in the present invention, as foods and drinks, for example, instant foods (immediate noodles, cup noodles, retort / cooked food, cooking canned food, microwave food, instant miso soup / soup, canned soup, freeze-dried food, etc. ), Carbonated drinks, citrus fruit juices (grapefruit, orange, lemon, etc.), juice drinks, soft drinks with juice, citrus fruit drinks and fruit drinks, tomatoes, peppers, celery, cucumbers, carrots, potatoes, asparagus Vegetable beverages including vegetables such as soy milk and soy milk beverages, coffee beverages, tea beverages, powdered beverages, concentrated beverages, sports beverages, nutritional beverages, alcoholic beverages, tobacco beverages and other favorite beverages and luxury products, bread, macaroni and spaghetti, Noodles, cake mix, deep-fried flour, bread crumbs, gyoza peel and other flour products, caramel・ Candies, chewing gum, chocolate, cookies / biscuits, cakes / pies, snacks / crackers, Japanese confectionery / rice confectionery / bean confectionery, dessert confectionery, soy sauce, miso, sauces, tomato processed seasonings, mirin, vinegar , Basic seasonings such as sweeteners, flavor seasonings, cooking mixes, curry ingredients, sauces, dressings, noodle soups, spices and other complex seasonings and foods, butter, margarines, mayonnaise, Fats and oils such as vegetable oil, milk / processed milk, milk drinks, yogurts, lactic acid bacteria drinks, cheese, ice creams, prepared powdered milk, milk and other dairy products such as cream, frozen material, semi-cooked frozen food, cooked frozen Frozen foods such as food, canned fish, canned fruits and pastes, fish ham and sausage, marine products, marine delicacy Processed fishery products such as dried fishery products, boiled fish, canned livestock cans / pastes, canned meat, canned fruits, jams / marmalades, pickles / boiled beans, dried agricultural products, processed cereals (cereal processed products) , Baby food, commercial foods such as sprinkles and ochazukenori.

  In addition, among the oral applied objects of the present invention, the pharmaceutical is not particularly limited as long as it can be contained in the mouth with the pharmaceuticals contained in the Japanese Pharmacopoeia. , Liquids, extracts, elixirs, capsules, granules, pills, powders, spirits, tablets, syrups, dip / decoction, troches, fragrances, limonases and the like. The quasi-drug is not particularly limited as long as it is a quasi-drug specified by the Minister of Health, Labor and Welfare and can be included in the mouth. For example, oral liquids, health drinks, disinfectants, antiseptics, vitamin-containing health care Agents and the like.

  The method for blending the lipid excretion promoter of the present invention into the oral application target is not particularly limited. For example, it may be performed by adding the tea polysaccharide of the present invention in a powder form or a liquid form in the preparation stage of the oral application target and homogenizing it. In addition, when preparing the oral application target, a coloring agent, an antioxidant, a fragrance, a corrigent, a surfactant, a solubilizing agent, a preservative, a sweetener, and the like may be used in combination as appropriate. Good.

  The blending amount of the lipid excretion promoter of the present invention with respect to the oral application target is not particularly limited, and it is preferable to appropriately set the blending amount according to the target oral application target. Generally, it is preferably 0.1% by weight or more in the final product, more preferably 1% by weight or more, and most preferably 5% by weight or more. The upper limit of the amount is preferably 50% by weight in the final product. If the amount of lipid excretion enhancer is less than 0.1% by weight, the intended effect of promoting lipid excretion may not be expected. On the other hand, when an excessive amount is blended, when the oral application target is a food or drink, the original taste of the food or drink is impaired, or a certain effect or more cannot be obtained, which is economically disadvantageous. There is.

  The lipid excretion enhancer of the present invention may be ingested by being blended with the oral application target as described above, but can also be ingested as it is. The intake amount varies depending on the intake form, age, weight, etc., but is not particularly limited.

  The lipid excretion enhancer of the present invention does not cause any problems even when used in combination with a conventionally known substance having a lipid excretion promoting action, such as tea catechin. When used in combination with these substances, a more excellent lipid excretion promoting effect can be expected.

  Hereinafter, the present invention will be described in more detail with reference to production examples, test examples and formulation examples. However, the present invention is not limited to this.

Production Example 1: Preparation of crude tea polysaccharide derived from green tea 5 kg of green tea was added to 50 L of hot water at 90 ° C. and extracted for 30 minutes with occasional stirring. The obtained extract was filtered, concentrated under reduced pressure, and then dried by spray drying to obtain about 1.5 kg of a powdery green tea extract.
2 L of 70% ethanol was added to 1.5 kg of the green tea extract obtained in this manner and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 200 g of a crude tea polysaccharide as an insoluble material.

Production Example 2: Preparation of purified tea polysaccharide derived from green tea (Part 1)
2 L of 70% ethanol was added to 1.5 kg of green tea hot water extract (Polyphenon G, manufactured by Tokyo Food Techno Co., Ltd.) and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 350 g of a crude tea polysaccharide as an insoluble material.
5 L of water was added to 350 g of this crude tea polysaccharide and stirred well to obtain a suspension. This suspension was subjected to ultrafiltration membrane (polysulfone membrane PS / 400K, manufactured by Spectrum) having a molecular weight cut off of 400,000. Thus, it was fractionated into a component having a molecular weight of 400,000 or more and a component having a molecular weight of less than 400,000. Each component obtained was concentrated and freeze-dried to obtain 222 g of a tea polysaccharide fraction having a molecular weight of 400,000 or more and 102 g of a tea polysaccharide fraction having a molecular weight of less than 400,000. The ratio of the constituent sugar contained in the tea polysaccharide having a molecular weight of 400,000 or more was such that the total constituent ratio of galacturonic acid, galactose and arabinose was about 30% by weight of the whole constituent sugar in the tea polysaccharide.

Production Example 3: Preparation of purified tea polysaccharide derived from green tea (part 2)
10 L of 70% ethanol was added to 2 kg of green tea hot water extract (Polyphenon G, manufactured by Tokyo Food Techno Co., Ltd.) and stirred well. The insoluble matter was collected by centrifugation, 4 L of 70% ethanol was added to the insoluble matter, and after sufficient stirring, the insoluble matter was collected by centrifugation. This operation was further repeated twice to obtain about 256 g of crude tea polysaccharide as an insoluble material.
10 g of water is added to 256 g of this crude tea polysaccharide and stirred well to obtain a suspension, and the molecular weight is less than 400,000 using an ultrafiltration membrane having a fractional molecular weight of 400,000 (polysulfone membrane PS / 400K, manufactured by Spectrum). These fractions were removed and fractions having a molecular weight of 400,000 or more were concentrated and collected.
Next, a precipitate was collected by centrifuging the obtained fraction having a molecular weight of 400,000 or more. The operation of washing the precipitate with 2 L of water was repeated twice and then freeze-dried to obtain about 118 g of insoluble tea polysaccharide having a molecular weight of 400,000 or more.
On the other hand, the water-soluble fraction obtained upon centrifugation was freeze-dried to obtain about 56 g of water-soluble tea polysaccharide having a molecular weight of 400,000 or more.
Further, the component having a molecular weight of less than 400,000 obtained by ultrafiltration was concentrated and freeze-dried to obtain about 50 g of tea polysaccharide having a molecular weight of less than 400,000.

Production Example 4: Preparation of purified tea polysaccharide derived from green tea (part 3)
2 L of 70% ethanol was added to 1.5 kg of green tea hot water extract (Polyphenon G, manufactured by Tokyo Food Techno Co., Ltd.) and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 350 g of a crude tea polysaccharide as an insoluble material.
5 L of water was added to 350 g of this crude tea polysaccharide and stirred well to obtain a suspension. This suspension was used with a microfiltration membrane (polypropylene membrane TCP-1, manufactured by Advantech) having a membrane pore size of 1.0 μm. Components larger than 1.0 μm were removed. The obtained permeate was fractionated into a component having a molecular weight of 400,000 or more and a component having a molecular weight of less than 400,000 using an ultrafiltration membrane having a fractional molecular weight of 400,000 (polysulfone membrane PS / 400K, manufactured by Spectrum). Each component obtained was concentrated and freeze-dried to obtain 222 g of a tea polysaccharide fraction having a molecular weight of 400,000 or more and 102 g of a tea polysaccharide fraction having a molecular weight of less than 400,000.

Production Example 5: Preparation of purified tea polysaccharide derived from Oolong tea 5 kg of Chinese Oolong tea was added to 50 L of hot water at 90 ° C. and extracted for 30 minutes with occasional stirring. The obtained extract was filtered, concentrated under reduced pressure, and then dried by spray drying to obtain about 1.5 kg of powdered oolong tea extract.
2 L of 70% ethanol was added to 1.5 kg of the oolong tea extract thus obtained and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 200 g of a crude tea polysaccharide as an insoluble material.
To 200 g of this crude tea polysaccharide, 4 L of water was added and stirred well to obtain a suspension. This suspension was subjected to ultrafiltration membrane (polysulfone membrane PS / 400K, And then concentrated and freeze-dried to obtain 87 g of a tea polysaccharide fraction having a molecular weight of 400,000 or more and 90 g of a tea polysaccharide fraction having a molecular weight of less than 400,000.

Production Example 6: Preparation of purified tea polysaccharide derived from black tea 5 kg of Indian black tea was added to 50 L of hot water at 90 ° C. and extracted for 30 minutes with occasional stirring. The obtained extract was filtered, concentrated under reduced pressure, and then dried by spray drying to obtain about 1.5 kg of a powdery black tea extract.
To 1.5 kg of the black tea extract thus obtained, 2 L of 70% ethanol was added and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 210 g of a crude tea polysaccharide as an insoluble material.
To 200 g of this crude tea polysaccharide, 4 L of water was added and stirred well to obtain a suspension. This suspension was subjected to ultrafiltration membrane (polysulfone membrane PS / 400K, And then concentrated and freeze-dried to obtain 80 g of a tea polysaccharide fraction having a molecular weight of 400,000 or more and 96 g of a tea polysaccharide fraction having a molecular weight of less than 400,000.

Production Example 7: Preparation of purified tea polysaccharide from green tea leaves To 1 kg of crushed green tea, 10 L of 70% aqueous ethanol solution was added and stirred at room temperature for 12 hours. Insoluble components were collected by filtration, and the same amount of 70% aqueous ethanol solution was added again and stirred for 8 hours. To the obtained insoluble component, 5 L of 70% ethanol aqueous solution was added and stirred for 4 hours to wash the insoluble component. This operation was repeated once more to obtain 500 g of ethanol-treated green tea leaves.
500 g of ethanol-treated green tea leaves were added to 5 L of hot water at 90 ° C., and extracted for 30 minutes with occasional stirring. The resulting extract 3L was treated with an ultrafiltration membrane having a fractional molecular weight of 400,000 or more (polysulfone membrane PS / 400K, manufactured by Spectrum), and 40 g of a water-soluble tea polysaccharide having a molecular weight of 400,000 or more and a molecular weight. 50 g of tea polysaccharide less than 400,000 was obtained.

Production Example 8: Preparation of purified tea polysaccharide derived from green tea (Part 4)
2 L of 70% ethanol was added to 1.5 kg of green tea hot water extract (Polyphenon G, manufactured by Tokyo Food Techno Co., Ltd.) and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 300 g of a crude tea polysaccharide as an insoluble material.
5 L of water is added to 300 g of this crude tea polysaccharide and stirred well to form a suspension. This suspension is subjected to a molecular weight of 200 using an ultrafiltration membrane (cellulose ester membrane, manufactured by Spectrum) having a molecular weight cut off of 2 million. It was fractionated into more than 10,000 components and less than 2 million components. Each component obtained was concentrated and freeze-dried to obtain 70 g of a tea polysaccharide fraction having a molecular weight of 2 million or more and 171 g of a tea polysaccharide fraction having a molecular weight of less than 2 million.

Test Example 1: Test for promoting lipid excretion by tea polysaccharide (Part 1)
The effect of promoting lipid excretion into feces by the crude tea polysaccharide described in Production Example 1 was examined according to the following test method.
(Test method)
Four-week-old male SD rats were preliminarily raised on a solid diet for 3 days, then divided into groups so that the average body weight was equal, and each group was fed a diet having the composition shown in Table 1 for 10 days. The crude tea polysaccharide described in Production Example 1 was added to the test group, and sucrose was added to the control group instead of the crude tea polysaccharide. During this time, food and water were freely consumed. For each group, feces were collected during the 10-day breeding period of all individuals, and the total cholesterol content, neutral fat content, total lipid content (total cholesterol and neutral fat content) and bile acids in feces The amount was measured and divided by the number of individuals to calculate the amount of each individual per 10 days. The calculation results are shown in Table 2 (phospholipids were excluded from the calculation results because the measurement amount was very small). The breeding room temperature was 23 ° C. with a light period of 8:00 to 20:00 and a dark period of 20:00 to 8:00.

(Test results)
As is apparent from Table 2, in the test group ingested the crude tea polysaccharide, the total amount of cholesterol in the feces, the amount of neutral fat, and the total amount of lipid were significantly increased compared to the control group. As a result, ingestion of the crude tea polysaccharide significantly promoted the excretion of lipid into feces, and exhibited an excellent lipid excretion promoting effect.
In the test group ingested with crude tea polysaccharide, the amount of bile acid was significantly increased compared to the control group. From this, it was confirmed that the crude tea polysaccharide has an action of increasing the amount of bile acids in feces, suggesting the possibility that bile acids have an effect on the excretion of lipids in the feces.

Test Example 2: Test for promoting lipid excretion by tea polysaccharide (Part 2)
The effect of promoting lipid excretion into feces by tea polysaccharide having a molecular weight of 400,000 or more as described in Production Example 2 was examined according to the following test method.
(Test method)
The same experiment as in Test Example 1 was performed using 4-week-old male SD rats. About the tea polysaccharide in feed, it changed into the tea polysaccharide which consists of a fraction with a molecular weight of 400,000 or more as described in manufacture example 2, and was made to ingest the component similar to test example 1 other than that (Table 3 below). reference). For each group, the feces during the 10-day breeding period of all individuals are collected, and the total cholesterol amount, neutral fat amount, phospholipid amount, total lipid amount (total cholesterol, neutral fat and phospholipids in the feces are determined). Total amount) and bile acid amounts were measured and divided by the number of individuals to calculate the amount of each individual per 10 days. Table 4 shows the calculation results. The breeding room temperature was 23 ° C. with a light period of 8:00 to 20:00 and a dark period of 20:00 to 8:00.

(Test results)
As is apparent from Table 4, in the test group ingested with a tea polysaccharide having a molecular weight of 400,000 or more, the total amount of cholesterol in the feces, the amount of triglycerides, the amount of phospholipids, and the total amount of lipids were all compared to the control group. It increased significantly and the effect was superior to the crude tea polysaccharide ingested in Test Example 1. From this, the ingestion of tea polysaccharides having a molecular weight of 400,000 or more significantly promoted the excretion of lipids into feces, and exhibited an excellent lipid excretion promoting effect.
In the test group ingested with a tea polysaccharide having a molecular weight of 400,000 or more, the amount of bile acids was significantly increased compared to the control group. This confirms that tea polysaccharides with a molecular weight of 400,000 or more have the effect of increasing the amount of bile acids in feces, suggesting that bile acids may have an effect on the excretion of lipids in feces. It was done.
Further, in addition to green tea-derived tea polysaccharides, similar tests were performed using Oolong tea-derived or black tea-derived tea polysaccharides. These polysaccharides also had the same effect. From this, it was confirmed that the tea polysaccharide used in the present invention is not limited to the type of tea and has an excellent lipid excretion promoting action.

  Below, the formulation example which mix | blended the lipid excretion promoter in this invention with the oral-application target object is shown. The food / beverage products containing the lipid excretion promoter of the present invention can be ingested deliciously without impairing the original taste / fragrance of the food / beverage products.

Formulation Example 1: Green tea beverage containing lipid excretion promoter 900 mL of water was heated to 60 ° C., and 30 g of green tea (gold) was added thereto and extracted for 6 minutes. After removing the tea husk with a 30-mesh strainer and cooling to 30 ° C. or lower, clarification was performed by filter paper filtration (industrial filter paper No. 26: ADVANTEC, collected particle size = 3 μm), and 760 mL of extract solution Got. This green tea extract is diluted with ion-exchanged water to a drinking concentration (catechin concentration 60 mg%), L-ascorbic acid is added to 0.03% by weight, and then pH 6.1 to 6. It adjusted to the range of 3 and was set as the preparation liquid. 5% by weight of the green tea-derived crude tea polysaccharide obtained in Production Example 1 was added to this blended solution and stirred well. These were hot-pack filled under a temperature condition of 80 ° C. or higher, and then sterilized at 121 ° C. for 10 minutes (F ₀ = 10 or higher) by retort sterilization. After cooling these to room temperature, a green tea beverage containing a lipid excretion promoter was obtained.

Formulation Example 2: Oolong tea drink containing lipid excretion promoter 900 mL of water was heated to 80 ° C., and 30 g of Oolong tea (Oolong G) was added thereto and extracted for 6 minutes. The tea shell was removed, cooled and clarified in the same manner as in Formulation Example 1 to obtain 810 mL of oolong tea extract. This oolong tea extract is diluted with ion-exchanged water so as to have a drinking concentration (catechin concentration of 50 mg%), L-ascorbic acid is added to 0.03% by weight, and then pH 6.1-6. It adjusted to the range of 3 and was set as the preparation liquid. To this blended solution, 5% by weight of the tea polysaccharide derived from Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5 was added and stirred well. These were hot-pack filled under a temperature condition of 80 ° C. or higher, and then sterilized at 121 ° C. for 10 minutes (F ₀ = 10 or higher) by retort sterilization. After these were cooled to room temperature, Oolong tea drink containing a lipid excretion promoter was obtained.

Formulation Example 3: Tea Beverage Containing Lipid Emission Promoter Prepared in the same manner as Formulation Example 2 except that Oolong Tea (Oolong G) in Formulation Example 2 was changed to black tea (Nuwaya Lemon Tea), The contained tea beverage was obtained.

Formulation Example 4: Barley tea beverage containing a lipid excretion enhancer 800 mL of water was heated to 90 ° C., and 40 g of barley tea raw barley was added thereto and extracted for 30 minutes. After removing the tea husk with a 30 mesh strainer and cooling to 30 ° C. or lower, clarification was performed by filter paper filtration (industrial filter paper No. 2: manufactured by ADVANTEC) to obtain 720 mL of an extract. This barley tea extract is diluted with ion-exchanged water so as to have a drinking concentration (Brix 0.4 °), L-ascorbic acid is added to 0.03% by weight, and the pH is 6.0 with sodium bicarbonate. To prepare a preparation liquid. To this blended solution, 5% by weight of the tea-derived tea polysaccharide having a molecular weight of 400,000 or more obtained in Production Example 6 was added and stirred well. These were hot-packed under a temperature condition of 80 ° C. or higher, and then sterilized at 121 ° C. for 20 minutes by retort sterilization. After cooling these to room temperature, a barley tea beverage containing a lipid excretion promoter was obtained.

Formulation Example 5: Mixed tea beverage containing lipid excretion promoter 900 mL of water is heated to 90 ° C. and mixed tea (mixing ratio: 65% oolong tea, 20% black tea, 5% jasmine, 4% crust, 4% hibiscus And Banaba 2%) were added and extracted for 10 minutes. After removing the tea husk with a 30-mesh strainer and cooling to 30 ° C. or lower, clarification was performed by filter paper filtration (industrial filter paper No. 2: manufactured by ADVANTEC) to obtain 820 mL of an extract. This mixed tea extract is diluted with ion-exchanged water to a drinking concentration (Brix 0.2 °), L-ascorbic acid is added to 0.03% by weight, and then pH 6.0 with sodium bicarbonate is added. It adjusted to the range and it was set as the preparation liquid. To this blended liquid, 5% by weight of green tea-derived tea polysaccharide having a molecular weight of 400,000 or more obtained in Production Example 2 was added and stirred well. These were hot-packed under a temperature condition of 80 ° C. or higher, and then sterilized at 121 ° C. for 20 minutes by retort sterilization. After cooling these to room temperature, a mixed tea beverage containing a lipid excretion promoter was obtained.

Formulation Example 6: Orange 50% fruit juice drink containing lipid excretion promoter 6 times concentrated orange fruit juice 84g
Fructose glucose liquid sugar 9.7g
Citric acid 0.06g
L-ascorbic acid 0.022g
5 g tea polysaccharide obtained in Production Example 5
Fragrance appropriate amount The above-described components were dissolved in ion-exchanged water to make a total amount of 1000 mL. This was filled in a container and sterilized by heating at 65 ° C. for 10 minutes to obtain an orange 50% fruit juice drink containing tea polysaccharides derived from Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5.

Formulation Example 7: Orange 100% juice drink containing lipid excretion promoter 1/6 Concentrated orange juice 168 g
10 g tea polysaccharide obtained in Production Example 6
Fragrance appropriate amount The above-described components were dissolved in ion-exchanged water to make a total amount of 1000 mL. This was filled in a container and sterilized by heating at 65 ° C. for 10 minutes to obtain a 100% orange juice drink containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6.

Formulation Example 8: Peach 50% fruit juice drink containing lipid excretion enhancer 1/4 concentrated peach juice 131g
Fructose glucose liquid sugar 9.7g
Citric acid 0.06g
L-ascorbic acid 0.022g
5 g tea polysaccharide obtained in Production Example 2
Fragrance appropriate amount The above-described components were dissolved in ion-exchanged water to make a total amount of 1000 mL. This was filled in a container and sterilized by heating at 65 ° C. for 10 minutes to obtain a peach 50% fruit juice drink containing tea polysaccharides derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2.

Formulation Example 9: Grapefruit juice containing lipid excretion promoter A commercially available grapefruit juice (Sanquist grapefruit juice concentrated and reduced 100%: trade name of Sanquist) derived from oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5 of the present invention 3% by weight of a tea polysaccharide was added to prepare a grapefruit juice containing a lipid excretion enhancer.

Formulation Example 10: Grapefruit juice with pulp containing lipid excretion promoter A tea derived from black tea with a molecular weight of 400,000 or more obtained in Production Example 6 of the present invention in commercially available grapefruit juice with pulp (Gokuri: trade name of Suntory) A grapefruit juice containing 3% by weight of polysaccharide and containing a lipid excretion enhancer was prepared.

Formulation Example 11: Vegetable juice containing a lipid excretion promoter A tea polysaccharide derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2 of the present invention in a commercially available vegetable juice (Kagome vegetable juice: trade name of Kagome Co., Ltd.) Was added to prepare a vegetable juice containing a lipid excretion enhancer.

Formulation Example 12: Coffee beverage containing lipid excretion promoter A tea derived from oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5 of the present invention in a commercially available coffee beverage (black bottle sugar-free: trade name of DyDo DRINCO) A 1% by weight polysaccharide was added to prepare a coffee drink containing a lipid excretion enhancer.

Formulation Example 13: Sports drink containing lipid excretion enhancer Tea polysaccharide obtained in Production Example 6 20 g
Vitamin B ₁ hydrochloride 0.45mg
Vitamin B ₂ 0.2mg
Vitamin C 10mg
Niacin 0.8mg
Pantothenic acid Ca 0.22mg
Ammonium iron citrate 12.57mg
Citric acid 100mg
Fructose 2.5g
Ion-exchanged water was added to the components described above to make a total volume of 200 mL, and a sports drink containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6 was prepared.

Formulation Example 14: Cola granulated sugar containing lipid excretion promoter 5g
Cola base 0.3g
Citric acid 0.05g
5 g tea polysaccharide obtained in Production Example 8
Carbonated water was added to the components described above to make the total amount 100 mL, and a cola containing green tea-derived tea polysaccharides having a molecular weight of 2 million or more obtained in Production Example 8 was prepared.

Formulation Example 15: 1.7 g milk shake butter containing lipid excretion promoter
Nonfat dry milk 5.5g
10 g tea polysaccharide obtained in Production Example 6
5g sugar
Sorbitol 4g
Emulsification stabilizer 0.7g
Ion exchange water was added to the components described above to make a total volume of 100 mL, and the mixture was dissolved by heating to 80 ° C. After homogenizing the milk fat, it was aged at 5 ° C. until the next day. After freezing this, it was rapidly cooled to −40 ° C. and mixed well to prepare a milk shake containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6.

Formulation Example 16: Drink yogurt containing lipid excretion promoter Breasted skim milk (fermented skim milk and ground) 40 g
13g sugar
Stabilizer 0.35g
Fragrance 0.05g
10 g tea polysaccharide obtained in Production Example 6
Ion exchange water was added to the components described above to make a total amount of 100 g, and mixed and dissolved. This was homogenized with a homogenizer to prepare a drink yogurt containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6.

Formulation Example 17: Red Wine Containing Lipid Excretion Promoter A commercially available red wine (Delica Maison Red / Light Type: trade name of Suntory) and obtained from Green Tea with a molecular weight of 400,000 or more obtained in Production Example 2 of the present invention A red wine containing 1% by weight of saccharide and containing a lipid excretion enhancer was prepared.

Formulation Example 18: Beer containing a lipid excretion promoter A commercially available beer (Kirin Lager: trade name of Kirin Brewery Co., Ltd.) containing 1 tea polysaccharide derived from Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5 of the present invention A beer was prepared containing% by weight and containing a lipid excretion promoter.

Formulation Example 19: Soymilk drink containing lipid excretion promoter Soy flour aqueous solution 500 g
90g sugar
5 times concentrated peach juice 20g
Citric acid 3g
Pectin 3g
Milk flavor 0.5g
1g peach flavor
10 g tea polysaccharide obtained in Production Example 6
The above-described components were mixed, and ion exchange water was added to make the total volume 1000 mL. The mixture was sterilized at 92 ° C. for 1 to 3 seconds, and then filled into a container at 87 ° C. to prepare a soy milk beverage containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6. .

Formulation Example 20: 30 g of soup corn starch containing a lipid excretion promoter
5 g tea polysaccharide obtained in Production Example 2
5g sugar
5g salt
Non-fat dry milk 30g
1g meat extract powder
4g of HAP powder
5g sodium glutamate
0.2g white pepper
Celery powder 0.01g
Onion extract powder 5g
Margarine 5g
5g dried carrot
0.3g dried parsley
The ingredients described above are powder-mixed in an instant soup formulation, 200 mL of water is added to 21.5 g of this mixture, heated for 3 to 5 minutes with good mixing, and derived from green tea with a molecular weight of 400,000 or more obtained in Production Example 2. A powder potage soup containing tea polysaccharides was prepared.

Formulation Example 21: Beef stew beef 110 g containing a lipid excretion promoter
Potato (6 slices) 60g
Carrot (6 slices) 50g
215g domiglas sauce
5 g tea polysaccharide obtained in Production Example 5
The above-mentioned materials were added to No. 4 can and mixed well. This was sterilized by retort at 115 ° C. for 90 minutes to prepare a beef stew containing tea polysaccharides derived from Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5.

Formulation Example 22: Retort curry containing a lipid excretion promoter Rose meat 12.4g
0.5g salt
5.2g butter
8.4g potato
Onion 12.4g
Carrot 2.5g
3.6g flour
Curry powder 0.5g
Spice 0.5g
5 g tea polysaccharide obtained in Production Example 5
Rose meat and vegetables were fried separately in a frying pan and placed in a pan. The retort curry containing tea polysaccharides derived from Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5 was prepared by frying the powder to make brown roux and simmering the whole in a pan.

Formulation Example 23: Non-oil dressing containing lipase promoter 30.8 g of vinegar
4g sugar
Salt 0.5g
8.4g thin soy sauce
Dashi soup 26.3g
20g powdered candy
10 g tea polysaccharide obtained in Production Example 6
After mixing the liquid raw material, the powder raw material was dissolved to prepare a non-oil dressing containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6.

Formulation Example 24: Noodle soup containing a lipid excretion promoter (double concentration type)
70mL soy sauce
20g sugar
Mirin 5mL
Boiled bonito broth 9g
Nucleic acid seasoning 3g
2g of salt
5 g tea polysaccharide obtained in Production Example 8
Caramel appropriate amount The above-described components were dissolved in ion-exchanged water to make a total amount of 1000 mL, and a double-concentrated noodle soup containing tea polysaccharides derived from green tea having a molecular weight of 2 million or more obtained in Production Example 8 was prepared.

Formulation Example 25: Yakiniku sauce containing lipid excretion promoter Soy sauce 900 mL
500g miso
350g sugar
Onion paste 100g
200g apple paste
60g sesame oil
250g garlic paste
20g white sesame paste
50 g of tea polysaccharide obtained in Production Example 2
The ingredients described above were dissolved in ion-exchanged water to make a total amount of 2000 mL, and a yakiniku sauce containing green tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 2 was prepared.

Formulation Example 26: Mayonnaise containing lipid excretion enhancer Egg yolk One egg Vinegar 1 teaspoon Salt Suitable amount Salad oil 120mL
Appropriate amount of sugar 5 g of tea polysaccharide obtained in Production Example 3
The ingredients described above were mixed while being slowly added according to a conventional method to prepare mayonnaise containing insoluble tea polysaccharide derived from green tea obtained in Production Example 3.

Formulation Example 27: Hard yogurt containing fat excretion promoter Nonfat milk 90.9 g
5g sugar
5 g tea polysaccharide obtained in Production Example 3
Stevioside 0.05g
Fragrance Small amount Curing agent Small amount After adding a curing agent to skim milk, 3% starter was inoculated and refrigerated when the acidity reached 0.7%. Other raw materials were stirred and mixed, and refrigerated again to prepare hard yogurt containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3.

Formulation Example 28: 7.5 g of ice cream butter containing a lipid excretion promoter
Nonfat dry milk 5g
10g sugar
5 g tea polysaccharide obtained in Production Example 3
Emulsification stabilizer 0.7g
Flour candy (DE 8) 7g
Ion exchange water was added to the components described above to make the total amount 100 g and mixed well. This was heated to 70 ° C., stirred with a homomixer, and then homogenized with a homogenizer. The ice cream containing the insoluble tea polysaccharide derived from the green tea obtained in Production Example 3 was prepared by aging in a refrigerator for 1 day and then rapidly cooling to −40 ° C. after freezing.

Formulation Example 29: Coffee whitener (powder type) containing lipid excretion promoter
Soybean hardened oil 50g
25g Minamata
Emulsifier 0.84g
Cream flavor 0.16g
20 g of tea polysaccharide obtained in Production Example 2
A water-soluble raw material was dissolved in 66.6% hot water with respect to the dry matter, and an emulsifier was dissolved in oil. Both were emulsified at 60 ° C., homogenized, and spray-dried to prepare a coffee whitener (powder type) containing tea polysaccharides derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2.

Formulation Example 30: Pickled seasoning liquid containing lipid excretion promoter Kelp extract 6g
4g bonito extract
4g of salt
1 g of tea polysaccharide obtained in Production Example 2
The said component was melt | dissolved in ion-exchange water, the whole quantity was 100g, and the seasoning liquid for pickles containing the polysaccharide derived from tea green tea with a molecular weight of 400,000 or more obtained by manufacture example 2 was prepared.

Formulation example 31: Jelly containing a lipid excretion promoter 1/5 Concentrated grape juice 10 g
20 g of tea polysaccharide obtained in Production Example 5
15g sugar
Glucose 20g
Minamata 12g
The above components were completely dissolved while heating with ion-exchanged water to make a total amount of 100 g. This was poured into a cup container, covered with a heat seal and cooled to prepare a jelly containing Oolong tea-derived tea polysaccharide having a molecular weight of 400,000 or more obtained in Production Example 5.

Formulation Example 32: Candy containing a lipid excretion promoter Sugar 50 g
Minamata 100g
Citric acid 1g
Perfume 0.4g
Coloring agent 0.1g
50 g tea polysaccharide obtained in Production Example 6
Thirty candies containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6 were prepared by a conventional method using the above-described components according to a candy formulation.

Formulation Example 33: Chewing gum containing a lipid excretion enhancer Vinyl acetate resin 20 g
Plasticizer 3g
Polyisobutylene 3g
Microcrystal wax 2g
Calcium carbonate 2g
40g sugar
Glucose 20g
10 g of tea polysaccharide obtained in Production Example 2
Aspartame 0.3g
Fragrance 1g
Ingredients other than sugar and fragrance were placed in a pan, heated and dissolved, and mixed well. Cooled to 50 ° C., added and mixed with sugar, added fragrance at 40 ° C., molded, allowed to cool, and contains tea polysaccharide derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2 A gum was prepared.

Formulation Example 34: Sweet chocolate bitter chocolate 33.2 g containing a lipid excretion promoter
50g sugar
10 g tea polysaccharide obtained in Production Example 8
Aspartame 0.31g
Cocoa butter 11.1g
The above-described components were mixed well at 40 ° C., and further kneaded for a long time with a reika machine to make the particles fine. It cooled after shaping | molding and the sweet chocolate containing the tea polysaccharide derived from the green tea with a molecular weight of 2 million or more obtained by manufacture example 8 was adjusted.

Formulation Example 35: Jam Orange peel containing lipid excretion promoter 250 g
100g sugar
Citric acid 0.5g
50 g of tea polysaccharide obtained in Production Example 2
The components described above were boiled in a conventional manner, and a jam containing tea polysaccharides derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2 was prepared.

Formulation Example 36: Cookie containing lipid excretion enhancer
74g starch
14g of water
15 g of tea polysaccharide obtained in Production Example 3
2 tsp baking powder
2 teaspoons of salt
1 egg butter 80g
2 tablespoons milk
Thirty cookies containing the green tea-derived insoluble tea polysaccharide obtained in Production Example 3 were prepared by the conventional method using the above-described cookie formulation.

Formulation Example 37: Custard cream containing a lipid excretion enhancer Upper white sugar 10 g
Glucose 5g
Minamata 6.2g
10 g of tea polysaccharide obtained in Production Example 2
3.8g of flour
Modified starch 7g
Nonfat dry milk 1.3g
Margarine 7.9g
Egg yolk (powder) 0.2g
Ion-exchanged water 41.9g
To a mixture of wheat flour, processed starch, egg yolk (powder) in a small amount of water, add the remaining water to 80 ° C and dissolve other ingredients, add it using a whisk and boil on high heat, A custard cream containing green tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 2 was prepared.

Formulation Example 38: 36.8 g of kojiyokan fresh sauce containing a lipid excretion promoter
Kanten 0.8g
7.2 g of water
40g sugar
10 g of tea polysaccharide obtained in Production Example 2
Water, sugar, and tea polysaccharides were added to the swelled water and dissolved by boiling under heating. After shaking this, it was boiled again, added with raw bean paste, and boiled until the total amount was 100 g. A kneaded candy containing green tea-derived tea polysaccharide having a molecular weight of 400,000 or more obtained in Production Example 2 was prepared.

Formulation Example 39: Bread containing a lipid excretion enhancer
3g dry yeast
Shortening 4g
Nonfat dry milk 5g
2g of salt
60g of water
10 g tea polysaccharide obtained in Production Example 3
The above ingredients were blended to prepare bread containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3 by a conventional method.

Formulation Example 40: Spaghetti flour containing lipid excretion promoter 100g
25 g of water
5 g tea polysaccharide obtained in Production Example 3
Powders were mixed and kneaded uniformly while adding water little by little to prepare spaghetti containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3.

Formulation Example 41: Chinese noodles containing a lipid excretion promoter Super-strong powder 70 g
Potato starch 30g
1g of salt
Kansui 2g
48g of water
10 g tea polysaccharide obtained in Production Example 3
Mixing the raw materials described above, kneading, forming, compounding, aging and rolling according to conventional methods to form a noodle band, cut into noodle strings, and containing the insoluble tea polysaccharide derived from green tea obtained in Production Example 3 Chinese noodles were prepared.

Formulation Example 42: Instant Chinese noodles containing lipid excretion promoter Commercially available Chinese noodle powder 60 g
Super strong powder 40g
Potato starch 12g
2g of salt
Commercial kansui 0.3g
35g of water
10 g tea polysaccharide obtained in Production Example 3
The raw materials described above were put into a mixer, mixed, rough rolled, rolled and cut out to obtain noodle strings. The noodle strings were steamed at 100 ° C. and fried in oil at 150 ° C. to prepare instant Chinese noodles containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3.

Formulation Example 43: Frozen udon noodles containing lipid excretion promoter 100g
3g of salt
30 g of water
10 g tea polysaccharide obtained in Production Example 3
The components described above were put in a mixer, mixed, aged, rolled, and cut out to obtain noodle strings. The noodle strings were put in boiling water, boiled for about 5 minutes, and cooled to 10 ° C. with cold water. After taking out the noodle strings for one ball, draining them, putting them in a container with a uniform thickness, quickly freezing at −30 ° C., and insoluble tea derived from green tea obtained in Production Example 3 Frozen udon containing polysaccharides was prepared.

Formulation Example 44: Spring roll skin containing lipid excretion promoter 99g wheat starch
1g flour
Emulsifier 3g
2g salad oil
Sake 2g
2g of salt
43g of water
2 g tea polysaccharide obtained in Production Example 8
The ingredients described above were stirred and kneaded to make a pasty dough, applied to a rotating drum to a thickness of 1 mm and baked, and the resulting dough was cut into 12 cm and 12 cm squares. A spring roll peel containing tea polysaccharides derived from green tea having a molecular weight of 2 million or more was prepared.

Formulation Example 45: Beef and pork sausage containing fat excretion promoter 22g beef
17.3g of water
Salt 1.7g
0.01g salting agent
1g sugar
Sodium glutamate 0.5g
Spice 0.5g
Horse glue 3g
Pork shoulder meat 54g
10 g tea polysaccharide obtained in Production Example 3
The above ingredients were ground and mixed as it was and filled into a film. After being left as salted at 5 ° C. for 12 hours, boiled at 75 ° C. for 90 minutes and then refrigerated, to prepare beef and pork sausage containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3.

Formulation Example 46: Kamaboko Surimi 60g containing a lipid excretion promoter
1.8g of salt
30.4g ice
6g starch
Seasoning 1.8g
6 g tea polysaccharide obtained in Production Example 3
The surimi, salt, and a small amount of ice were mixed, and break mixing was performed with a silent cutter for 5 minutes, and then the remaining ice and raw materials were added and mixed for 10 minutes. The mixture was finished when it became sticky at 15 ° C., molded, and fried with 160 ° C. oil for 4 minutes to prepare kamaboko containing the green tea-derived insoluble tea polysaccharide obtained in Production Example 3.

Formulation Example 47: Oral solution containing lipid excretion enhancer Tea polysaccharide obtained in Production Example 2 10 g
Carrot dry extract 214mg
Dried licorice extract 50mg
Ursodeoxycholic acid 25mg
D-sorbitol 50g
25g white sugar
Polyoxyethylene hydrogenated castor oil 3g
Propylene glycol 5mL
Sodium benzoate 600mg
Butyl paraoxybenzoate 10mg
Citrate buffer appropriate amount Ion-exchanged water was added to the above components to make the total amount 300 mL, and an internal liquid solution containing tea polysaccharide derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2 was prepared.

  The lipid excretion enhancer of the present invention can be added to various oral application objects as described above.

Claims (3)

  A lipid excretion promoter characterized by comprising tea-derived polysaccharide as an active ingredient.   The lipid excretion promoter according to claim 1, wherein the polysaccharide has a molecular weight of 400,000 or more.   An object to be applied to the oral cavity, comprising an effective amount of the lipid excretion promoter according to claim 1.