JP2005095144A - 生物学的分子に結合した化学基を測定する方法 - Google Patents

生物学的分子に結合した化学基を測定する方法 Download PDF

Info

Publication number
JP2005095144A
JP2005095144A JP2004102903A JP2004102903A JP2005095144A JP 2005095144 A JP2005095144 A JP 2005095144A JP 2004102903 A JP2004102903 A JP 2004102903A JP 2004102903 A JP2004102903 A JP 2004102903A JP 2005095144 A JP2005095144 A JP 2005095144A
Authority
JP
Japan
Prior art keywords
biomolecule
fluorescence lifetime
sample
biological molecule
fluorescent marker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2004102903A
Other languages
English (en)
Japanese (ja)
Other versions
JP2005095144A5 (enExample
Inventor
Klaus Doering
クラウス・デーリング
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tecan Trading AG
Original Assignee
Tecan Trading AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tecan Trading AG filed Critical Tecan Trading AG
Publication of JP2005095144A publication Critical patent/JP2005095144A/ja
Publication of JP2005095144A5 publication Critical patent/JP2005095144A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/80Fluorescent dyes, e.g. rhodamine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2004102903A 2003-04-10 2004-03-31 生物学的分子に結合した化学基を測定する方法 Pending JP2005095144A (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH6532003 2003-04-10

Publications (2)

Publication Number Publication Date
JP2005095144A true JP2005095144A (ja) 2005-04-14
JP2005095144A5 JP2005095144A5 (enExample) 2007-05-24

Family

ID=32855168

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2004102903A Pending JP2005095144A (ja) 2003-04-10 2004-03-31 生物学的分子に結合した化学基を測定する方法

Country Status (5)

Country Link
US (1) US7223610B2 (enExample)
EP (1) EP1467211B1 (enExample)
JP (1) JP2005095144A (enExample)
AT (1) ATE393915T1 (enExample)
DE (1) DE502004006979D1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007509317A (ja) * 2003-10-18 2007-04-12 バイエル・ヘルスケア・アクチェンゲゼルシャフト 生物学的試験システムにおける、蛍光寿命の測定による分子修飾の直接的観察

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005523024A (ja) * 2002-04-19 2005-08-04 アマシャム バイオサイエンス ユーケイ リミテッド プロテインキナーゼ及びホスファターゼ活性の測定法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1994800A (en) * 1999-01-15 2000-08-01 Pantherix Ltd. Phosphate release enzyme assay
US6410255B1 (en) * 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US6287774B1 (en) * 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005523024A (ja) * 2002-04-19 2005-08-04 アマシャム バイオサイエンス ユーケイ リミテッド プロテインキナーゼ及びホスファターゼ活性の測定法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007509317A (ja) * 2003-10-18 2007-04-12 バイエル・ヘルスケア・アクチェンゲゼルシャフト 生物学的試験システムにおける、蛍光寿命の測定による分子修飾の直接的観察

Also Published As

Publication number Publication date
ATE393915T1 (de) 2008-05-15
US20040259183A1 (en) 2004-12-23
DE502004006979D1 (de) 2008-06-12
US7223610B2 (en) 2007-05-29
EP1467211A1 (de) 2004-10-13
EP1467211B1 (de) 2008-04-30

Similar Documents

Publication Publication Date Title
US20240003892A1 (en) Heterogeneous single cell profiling using molecular barcoding
EP1451348B1 (en) Methods, reagents, kits and apparatus for protein function analysis
US9746476B2 (en) Real-time analytical methods and systems
Zaman et al. Fluorescence assays for high-throughput screening of protein kinases
Unger-Angel et al. Protein recognition by bivalent,‘turn-on’fluorescent molecular probes
EP2812698B1 (en) Dual-acceptor time-resolved-fret
Klumpp et al. Readout technologies for highly miniaturized kinase assays applicable to high-throughput screening in a 1536-well format
Von Leoprechting et al. Miniaturization and validation of a high-throughput serine kinase assay using the alpha screen platform
JP5085320B2 (ja) 生体反応又は生体内状態変化の複数同時解析法
US9146233B2 (en) Detecting molecular interactions by fluorescence resonance energy transfer on a solid-phase support
US20100041068A1 (en) Deeply quenched enzyme sensors and binding sensors
JP2005095144A (ja) 生物学的分子に結合した化学基を測定する方法
WO2009069980A2 (en) Protein chip for determining kinase or phosphatase activity
JP2008104356A (ja) リン酸化−脱リン酸化反応検出用基質群およびそれを用いた検出方法
Tang et al. The fantastic single-molecule techniques
US7795040B2 (en) Methods, particles, and kits for determining activity of a kinase
US8309298B2 (en) Method for detecting protein
Ploetz et al. Fluorescence resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers
Margulies et al. Protein surface recognition with targeted fluorescent molecular probes
Zhu et al. 14 A Combined Force of Chemical Genetics and Protein Microarrays
Visalli et al. Microarrays as a Tool for Gene Expression Profiling: Application in Ocular and kk
JP2006512905A (ja) ポリ(adp−リボース)ポリメラーゼ(parp)の活性を減少させる化合物を検定する方法
JP2005095144A5 (enExample)
HK1098533A (en) Direct observation of molecular modifications in biological test systems by measuring fluorescence lifetime
CA2714094A1 (en) Methods and apparatus for conducting multiple measurements on a sample

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20070328

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20070328

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100223

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20100519

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20100524

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20100623

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20100628

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20101005