JP2005052142A - Rna単離用の装置及び方法 - Google Patents
Rna単離用の装置及び方法 Download PDFInfo
- Publication number
- JP2005052142A JP2005052142A JP2004221269A JP2004221269A JP2005052142A JP 2005052142 A JP2005052142 A JP 2005052142A JP 2004221269 A JP2004221269 A JP 2004221269A JP 2004221269 A JP2004221269 A JP 2004221269A JP 2005052142 A JP2005052142 A JP 2005052142A
- Authority
- JP
- Japan
- Prior art keywords
- column
- rna
- sample
- silicon carbide
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Filtering Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/631,189 US20050026153A1 (en) | 2003-07-31 | 2003-07-31 | Devices and methods for isolating RNA |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005052142A true JP2005052142A (ja) | 2005-03-03 |
| JP2005052142A5 JP2005052142A5 (enExample) | 2007-09-13 |
Family
ID=33541514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004221269A Pending JP2005052142A (ja) | 2003-07-31 | 2004-07-29 | Rna単離用の装置及び方法 |
Country Status (3)
| Country | Link |
|---|---|
| US (2) | US20050026153A1 (enExample) |
| EP (1) | EP1502951A1 (enExample) |
| JP (1) | JP2005052142A (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008005839A (ja) * | 2006-06-29 | 2008-01-17 | Millipore Corp | 生物学的試料からのrna及びdnaの単離 |
| US7824855B2 (en) | 2004-03-26 | 2010-11-02 | Fujifilm Corporation | Method for selectively separating and purifying RNA and method for separating and purifying nucleic acid |
| JP2022546519A (ja) * | 2019-08-28 | 2022-11-04 | コンタミネーション ソース アイデンティフィケーション エルエルシー | 生物試料に由来する微生物rnaの迅速な分離と収集 |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7569342B2 (en) * | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
| US20080064108A1 (en) * | 1997-12-10 | 2008-03-13 | Tony Baker | Urine Preservation System |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| US20050042660A1 (en) * | 2003-07-31 | 2005-02-24 | Hall Gerald Edward | Devices and methods for isolating RNA |
| US20060099605A1 (en) * | 2004-11-11 | 2006-05-11 | Hall Gerald E Jr | Devices and methods for isolating RNA |
| US20060270843A1 (en) * | 2005-05-26 | 2006-11-30 | Hall Gerald E Jr | Methods for isolation of nucleic acids |
| EP1772522A1 (en) * | 2005-10-04 | 2007-04-11 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | Control of preservation by biomarkers |
| US20070116613A1 (en) * | 2005-11-23 | 2007-05-24 | Donat Elsener | Sample tube and system for storing and providing nucleic acid samples |
| US20080044851A1 (en) * | 2006-06-02 | 2008-02-21 | Epicentre Technologies | Compositions and methods for removal of DNA from a sample |
| US8686129B2 (en) * | 2007-03-20 | 2014-04-01 | Agilent Technologies, Inc. | Methods for the separation of biological molecules using sulfolane |
| GB2505223B (en) * | 2012-08-23 | 2014-11-19 | Thermo Fisher Scientific Baltics Uab | Spin column |
| AU2013310861B2 (en) * | 2012-09-03 | 2019-02-14 | Qiagen Gmbh | Method for isolating RNA including small RNA with high yield |
| US9347056B2 (en) * | 2012-10-26 | 2016-05-24 | Seiko Epson Corporation | Nucleic acid extraction device, and nucleic acid extraction method, nucleic acid extraction kit, and nucleic acid extraction apparatus, each using the same |
| GB2581489B (en) * | 2019-02-15 | 2021-02-24 | Revolugen Ltd | Purification method |
| DE102019108087A1 (de) | 2019-03-28 | 2020-10-01 | Axagarius Gmbh & Co. Kg | Verfahren zur Isolierung von Nukleinsäuren aus Probematerialien |
| DE102020109837B3 (de) | 2020-04-08 | 2020-12-31 | Axagarius Gmbh & Co. Kg | Isolierung von Nukleinsäuren aus Umweltproben mittels magnetischer Partikel |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000009680A1 (en) * | 1998-08-10 | 2000-02-24 | Chiron Corporation | Methods for purifying non-chromosomal nucleic acid molecules from cells |
| US6043354A (en) * | 1996-01-31 | 2000-03-28 | Invitek Gmbh | Method for the simultaneous isolation of genomic DNA and high-purity RNA |
| JP2002507121A (ja) * | 1997-06-25 | 2002-03-05 | プロメガ コーポレイション | Rnaの単離方法 |
| JP2002542780A (ja) * | 1999-04-23 | 2002-12-17 | ハジ−アハマド ユーセフ | 炭化ケイ素を使用した核酸精製法 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3414394A (en) * | 1965-06-21 | 1968-12-03 | Owens Illinois Inc | Sintered glass article and method of making |
| SE377108B (enExample) * | 1970-03-27 | 1975-06-23 | Kanegafuchi Spinning Co Ltd | |
| US4996144A (en) * | 1985-01-25 | 1991-02-26 | Calgene, Inc. | Microassay for detection of DNA and RNA |
| US5869620A (en) * | 1986-09-02 | 1999-02-09 | Enzon, Inc. | Multivalent antigen-binding proteins |
| US5006472A (en) * | 1988-06-03 | 1991-04-09 | Miles Inc. | Enzymatic purification process |
| US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US5437976A (en) * | 1991-08-08 | 1995-08-01 | Arizona Board Of Regents, The University Of Arizona | Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA |
| US5480972A (en) * | 1992-10-30 | 1996-01-02 | The University Of Melbourne | Allergenic proteins from Johnson grass pollen |
| DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
| US5637687A (en) * | 1993-08-31 | 1997-06-10 | Wiggins; James C. | Methods and compositions for isolating nucleic acids |
| US5945515A (en) * | 1995-07-31 | 1999-08-31 | Chomczynski; Piotr | Product and process for isolating DNA, RNA and proteins |
| US5922536A (en) * | 1996-10-18 | 1999-07-13 | Nivens; David E. | Method for nucleic acid isolation using supercritical fluids |
| JP2001522238A (ja) * | 1997-03-31 | 2001-11-13 | アボツト・ラボラトリーズ | 胃腸管の疾患の検出に有用な試薬および方法 |
| US6103192A (en) * | 1997-04-14 | 2000-08-15 | Genetec Corporation | Immobilizing and processing specimens on matrix materials for the identification of nucleic acid sequences |
| US6897066B1 (en) * | 1997-09-26 | 2005-05-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
| DE19746874A1 (de) * | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen |
| ZA99493B (en) * | 1998-01-30 | 1999-07-22 | Akzo Nobel Nv | Method for the isolation of nucleic acid. |
| US6310199B1 (en) * | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| US6270970B1 (en) * | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
| US6548256B2 (en) * | 2000-07-14 | 2003-04-15 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| ATE342355T1 (de) * | 2000-11-13 | 2006-11-15 | Promega Corp | Lysat-klärung und nukleinsäureisolierung mit silanisierten silicamatrizes |
-
2003
- 2003-07-31 US US10/631,189 patent/US20050026153A1/en not_active Abandoned
- 2003-10-24 US US10/693,428 patent/US20050026159A1/en not_active Abandoned
-
2004
- 2004-06-30 EP EP04015364A patent/EP1502951A1/en not_active Withdrawn
- 2004-07-29 JP JP2004221269A patent/JP2005052142A/ja active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6043354A (en) * | 1996-01-31 | 2000-03-28 | Invitek Gmbh | Method for the simultaneous isolation of genomic DNA and high-purity RNA |
| JP2002507121A (ja) * | 1997-06-25 | 2002-03-05 | プロメガ コーポレイション | Rnaの単離方法 |
| WO2000009680A1 (en) * | 1998-08-10 | 2000-02-24 | Chiron Corporation | Methods for purifying non-chromosomal nucleic acid molecules from cells |
| JP2002542780A (ja) * | 1999-04-23 | 2002-12-17 | ハジ−アハマド ユーセフ | 炭化ケイ素を使用した核酸精製法 |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7824855B2 (en) | 2004-03-26 | 2010-11-02 | Fujifilm Corporation | Method for selectively separating and purifying RNA and method for separating and purifying nucleic acid |
| JP2008005839A (ja) * | 2006-06-29 | 2008-01-17 | Millipore Corp | 生物学的試料からのrna及びdnaの単離 |
| JP2008054660A (ja) * | 2006-06-29 | 2008-03-13 | Millipore Corp | 核酸を単離するためのろ過装置 |
| JP2022546519A (ja) * | 2019-08-28 | 2022-11-04 | コンタミネーション ソース アイデンティフィケーション エルエルシー | 生物試料に由来する微生物rnaの迅速な分離と収集 |
| JP7725453B2 (ja) | 2019-08-28 | 2025-08-19 | コンタミネーション ソース アイデンティフィケーション エルエルシー | 生物試料に由来する微生物rnaの迅速な分離と収集 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20050026153A1 (en) | 2005-02-03 |
| EP1502951A1 (en) | 2005-02-02 |
| US20050026159A1 (en) | 2005-02-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2142910C (en) | A method for the purification and separation of nucleic acid mixtures by chromatography | |
| EP2539449B1 (en) | Process for parallel isolation and/or purification of rna and dna | |
| AU745185B2 (en) | Method of isolating RNA | |
| JP2005052142A (ja) | Rna単離用の装置及び方法 | |
| JP2005052142A5 (enExample) | ||
| US6037465A (en) | Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials | |
| JP6096660B2 (ja) | 低分子標的核酸を含む標的核酸を高収量で単離するための方法 | |
| EP1618194B1 (en) | Compositions and methods for using a solid support to purify rna | |
| CN101124321B (zh) | 用于从稳定剂中纯化核酸的组合物和方法 | |
| CN102812118B (zh) | 分离和纯化核酸的方法 | |
| WO2007008722A2 (en) | Method for the isolation of rna from biological sources | |
| CN102822340A (zh) | 分离和纯化核酸的色谱设备和方法 | |
| JP2005151975A (ja) | Rna単離用の方法と装置 | |
| JP3979996B2 (ja) | 固体支持体を使用してrnaを精製するための組成物および方法 | |
| US20060099605A1 (en) | Devices and methods for isolating RNA | |
| CN116555247B (zh) | 一种核酸提取方法 | |
| EP1526176A2 (en) | Devices and methods for isolating RNA | |
| WO2005093065A1 (en) | Improved method of isolating nucleic acids | |
| US20050042660A1 (en) | Devices and methods for isolating RNA | |
| EP1626085A1 (en) | Devices and methods for isolating RNA | |
| US20100291658A1 (en) | Process for separating nonproteinaneous biomolecules, in particular nucleic acids, from proteinaneous samples | |
| US20060223072A1 (en) | Methods of using a DNase I-like enzyme | |
| EP2556156A1 (en) | Method for selective isolation and purification of nucleic acids |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070730 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070730 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20100518 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20101019 |