JP2004535773A5 - - Google Patents
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- Publication number
- JP2004535773A5 JP2004535773A5 JP2002563325A JP2002563325A JP2004535773A5 JP 2004535773 A5 JP2004535773 A5 JP 2004535773A5 JP 2002563325 A JP2002563325 A JP 2002563325A JP 2002563325 A JP2002563325 A JP 2002563325A JP 2004535773 A5 JP2004535773 A5 JP 2004535773A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- acid molecule
- sequence
- molecule
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims description 66
- 108020004707 nucleic acids Proteins 0.000 claims description 66
- 102000039446 nucleic acids Human genes 0.000 claims description 66
- 150000007523 nucleic acids Chemical class 0.000 claims description 66
- 108090000623 proteins and genes Proteins 0.000 claims description 29
- 102000004169 proteins and genes Human genes 0.000 claims description 24
- 238000000137 annealing Methods 0.000 claims description 19
- 210000004027 cell Anatomy 0.000 claims description 13
- 241000894007 species Species 0.000 claims description 13
- 239000012634 fragment Substances 0.000 claims description 11
- 210000000349 chromosome Anatomy 0.000 claims description 8
- 108020004414 DNA Proteins 0.000 claims description 7
- 102000053602 DNA Human genes 0.000 claims description 7
- 108091034117 Oligonucleotide Proteins 0.000 claims description 4
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 claims description 3
- 239000013612 plasmid Substances 0.000 claims description 3
- 241000203069 Archaea Species 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 241000700605 Viruses Species 0.000 claims description 2
- 238000012217 deletion Methods 0.000 claims description 2
- 230000037430 deletion Effects 0.000 claims description 2
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 2
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 244000045947 parasite Species 0.000 claims description 2
- 238000006467 substitution reaction Methods 0.000 claims description 2
- 238000005215 recombination Methods 0.000 claims 7
- 230000006798 recombination Effects 0.000 claims 7
- 101100226347 Escherichia phage lambda exo gene Proteins 0.000 claims 6
- 238000006011 modification reaction Methods 0.000 claims 4
- 210000001236 prokaryotic cell Anatomy 0.000 claims 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 3
- 238000010367 cloning Methods 0.000 claims 3
- 241000588724 Escherichia coli Species 0.000 claims 2
- 101100316841 Escherichia phage lambda bet gene Proteins 0.000 claims 2
- 230000001580 bacterial effect Effects 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 241000219194 Arabidopsis Species 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- 241001646716 Escherichia coli K-12 Species 0.000 claims 1
- 241000701959 Escherichia virus Lambda Species 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 241000018427 Iphisa elegans Species 0.000 claims 1
- 241000588748 Klebsiella Species 0.000 claims 1
- 241000588653 Neisseria Species 0.000 claims 1
- 102000001218 Rec A Recombinases Human genes 0.000 claims 1
- 108010055016 Rec A Recombinases Proteins 0.000 claims 1
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 230000000692 anti-sense effect Effects 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000004520 electroporation Methods 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 238000001727 in vivo Methods 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 239000000178 monomer Substances 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 230000003071 parasitic effect Effects 0.000 claims 1
- 239000002994 raw material Substances 0.000 claims 1
- 230000013120 recombinational repair Effects 0.000 claims 1
- 230000010076 replication Effects 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 238000012546 transfer Methods 0.000 claims 1
- 230000009466 transformation Effects 0.000 claims 1
- 238000011426 transformation method Methods 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
- 210000004436 artificial bacterial chromosome Anatomy 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000269368 Xenopus laevis Species 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000012872 hydroxylapatite chromatography Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0103276A GB0103276D0 (en) | 2001-02-09 | 2001-02-09 | Recombination method |
| GB0120312A GB0120312D0 (en) | 2001-08-21 | 2001-08-21 | Recombination method |
| PCT/IB2002/001415 WO2002062988A2 (en) | 2001-02-09 | 2002-02-08 | Recombination method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004535773A JP2004535773A (ja) | 2004-12-02 |
| JP2004535773A5 true JP2004535773A5 (enExample) | 2008-06-05 |
| JP4355142B2 JP4355142B2 (ja) | 2009-10-28 |
Family
ID=26245704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002563325A Expired - Fee Related JP4355142B2 (ja) | 2001-02-09 | 2002-02-08 | 組換え法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20040101520A1 (enExample) |
| EP (1) | EP1399546A2 (enExample) |
| JP (1) | JP4355142B2 (enExample) |
| CA (1) | CA2436743A1 (enExample) |
| WO (1) | WO2002062988A2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1311661T3 (da) | 2000-08-14 | 2012-11-26 | Us Gov Health & Human Serv | Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner |
| US7521242B2 (en) | 2003-05-09 | 2009-04-21 | The United States Of America As Represented By The Department Of Health And Human Services | Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids |
| US7674621B2 (en) | 2004-05-21 | 2010-03-09 | The United States Of America As Represented By The Department Of Health And Human Services | Plasmids and phages for homologous recombination and methods of use |
| US20070281309A1 (en) * | 2006-05-19 | 2007-12-06 | Massachusetts Institute Of Technology | Microfluidic-based Gene Synthesis |
| GB0909660D0 (en) | 2009-06-04 | 2009-07-22 | Gene Bridges Gmbh | Method of altering nucleic acids |
| GB0922108D0 (en) | 2009-12-17 | 2010-02-03 | Gene Bridges Gmbh | Heterologous hosts |
| GB201009732D0 (en) | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| CN103667331B (zh) * | 2013-12-10 | 2015-09-30 | 南京师范大学 | 重组酶基因bet作为一种大肠杆菌异源蛋白表达融合标签的应用 |
| JP6899564B2 (ja) * | 2016-08-12 | 2021-07-07 | Nexuspiral株式会社 | ゲノム編集方法 |
| CN106995813B (zh) | 2017-03-23 | 2020-06-16 | 山东大学 | 基因组大片段直接克隆和dna多分子组装新技术 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE449844T1 (de) * | 1997-12-05 | 2009-12-15 | Europ Lab Molekularbiolog | Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems |
| DK1311661T3 (da) * | 2000-08-14 | 2012-11-26 | Us Gov Health & Human Serv | Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner |
-
2002
- 2002-02-08 EP EP02722618A patent/EP1399546A2/en not_active Withdrawn
- 2002-02-08 US US10/470,679 patent/US20040101520A1/en not_active Abandoned
- 2002-02-08 WO PCT/IB2002/001415 patent/WO2002062988A2/en not_active Ceased
- 2002-02-08 CA CA002436743A patent/CA2436743A1/en not_active Abandoned
- 2002-02-08 JP JP2002563325A patent/JP4355142B2/ja not_active Expired - Fee Related
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