CA2436743A1 - Recombination method - Google Patents

Recombination method Download PDF

Info

Publication number
CA2436743A1
CA2436743A1 CA002436743A CA2436743A CA2436743A1 CA 2436743 A1 CA2436743 A1 CA 2436743A1 CA 002436743 A CA002436743 A CA 002436743A CA 2436743 A CA2436743 A CA 2436743A CA 2436743 A1 CA2436743 A1 CA 2436743A1
Authority
CA
Canada
Prior art keywords
nucleic acid
acid molecule
recombination
sequence
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002436743A
Other languages
English (en)
French (fr)
Inventor
Joep P. P. Muijrers
Youming Zhang
Adrian Francis Stewart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gene Bridges GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0103276A external-priority patent/GB0103276D0/en
Priority claimed from GB0120312A external-priority patent/GB0120312D0/en
Application filed by Individual filed Critical Individual
Publication of CA2436743A1 publication Critical patent/CA2436743A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002436743A 2001-02-09 2002-02-08 Recombination method Abandoned CA2436743A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0103276A GB0103276D0 (en) 2001-02-09 2001-02-09 Recombination method
GB0103276.2 2001-02-09
GB0120312.4 2001-08-21
GB0120312A GB0120312D0 (en) 2001-08-21 2001-08-21 Recombination method
PCT/IB2002/001415 WO2002062988A2 (en) 2001-02-09 2002-02-08 Recombination method

Publications (1)

Publication Number Publication Date
CA2436743A1 true CA2436743A1 (en) 2002-08-15

Family

ID=26245704

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002436743A Abandoned CA2436743A1 (en) 2001-02-09 2002-02-08 Recombination method

Country Status (5)

Country Link
US (1) US20040101520A1 (enExample)
EP (1) EP1399546A2 (enExample)
JP (1) JP4355142B2 (enExample)
CA (1) CA2436743A1 (enExample)
WO (1) WO2002062988A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1311661T3 (da) 2000-08-14 2012-11-26 Us Gov Health & Human Serv Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner
US7521242B2 (en) 2003-05-09 2009-04-21 The United States Of America As Represented By The Department Of Health And Human Services Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
US7674621B2 (en) 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
US20070281309A1 (en) * 2006-05-19 2007-12-06 Massachusetts Institute Of Technology Microfluidic-based Gene Synthesis
GB0909660D0 (en) 2009-06-04 2009-07-22 Gene Bridges Gmbh Method of altering nucleic acids
GB0922108D0 (en) 2009-12-17 2010-02-03 Gene Bridges Gmbh Heterologous hosts
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
CN103667331B (zh) * 2013-12-10 2015-09-30 南京师范大学 重组酶基因bet作为一种大肠杆菌异源蛋白表达融合标签的应用
JP6899564B2 (ja) * 2016-08-12 2021-07-07 Nexuspiral株式会社 ゲノム編集方法
CN106995813B (zh) 2017-03-23 2020-06-16 山东大学 基因组大片段直接克隆和dna多分子组装新技术

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE449844T1 (de) * 1997-12-05 2009-12-15 Europ Lab Molekularbiolog Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems
DK1311661T3 (da) * 2000-08-14 2012-11-26 Us Gov Health & Human Serv Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner

Also Published As

Publication number Publication date
JP4355142B2 (ja) 2009-10-28
JP2004535773A (ja) 2004-12-02
EP1399546A2 (en) 2004-03-24
WO2002062988A2 (en) 2002-08-15
WO2002062988A3 (en) 2004-01-15
US20040101520A1 (en) 2004-05-27

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued