JP4355142B2 - 組換え法 - Google Patents

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Publication number
JP4355142B2
JP4355142B2 JP2002563325A JP2002563325A JP4355142B2 JP 4355142 B2 JP4355142 B2 JP 4355142B2 JP 2002563325 A JP2002563325 A JP 2002563325A JP 2002563325 A JP2002563325 A JP 2002563325A JP 4355142 B2 JP4355142 B2 JP 4355142B2
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JP
Japan
Prior art keywords
nucleic acid
acid molecule
recombination
protein
sequence
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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JP2002563325A
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English (en)
Japanese (ja)
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JP2004535773A (ja
JP2004535773A5 (enExample
Inventor
ジョープ ピー. ピー. ムイジュラース,
ユーミン ザン,
アドリアン フランシス スチュアート,
Original Assignee
ジーン ブリッジス ゲゼルシャフト ミット ベシュレンクテル ハフツング
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Publication date
Priority claimed from GB0103276A external-priority patent/GB0103276D0/en
Priority claimed from GB0120312A external-priority patent/GB0120312D0/en
Application filed by ジーン ブリッジス ゲゼルシャフト ミット ベシュレンクテル ハフツング filed Critical ジーン ブリッジス ゲゼルシャフト ミット ベシュレンクテル ハフツング
Publication of JP2004535773A publication Critical patent/JP2004535773A/ja
Publication of JP2004535773A5 publication Critical patent/JP2004535773A5/ja
Application granted granted Critical
Publication of JP4355142B2 publication Critical patent/JP4355142B2/ja
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2002563325A 2001-02-09 2002-02-08 組換え法 Expired - Fee Related JP4355142B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0103276A GB0103276D0 (en) 2001-02-09 2001-02-09 Recombination method
GB0120312A GB0120312D0 (en) 2001-08-21 2001-08-21 Recombination method
PCT/IB2002/001415 WO2002062988A2 (en) 2001-02-09 2002-02-08 Recombination method

Publications (3)

Publication Number Publication Date
JP2004535773A JP2004535773A (ja) 2004-12-02
JP2004535773A5 JP2004535773A5 (enExample) 2008-06-05
JP4355142B2 true JP4355142B2 (ja) 2009-10-28

Family

ID=26245704

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2002563325A Expired - Fee Related JP4355142B2 (ja) 2001-02-09 2002-02-08 組換え法

Country Status (5)

Country Link
US (1) US20040101520A1 (enExample)
EP (1) EP1399546A2 (enExample)
JP (1) JP4355142B2 (enExample)
CA (1) CA2436743A1 (enExample)
WO (1) WO2002062988A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1311661T3 (da) 2000-08-14 2012-11-26 Us Gov Health & Human Serv Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner
US7521242B2 (en) 2003-05-09 2009-04-21 The United States Of America As Represented By The Department Of Health And Human Services Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
US7674621B2 (en) 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
US20070281309A1 (en) * 2006-05-19 2007-12-06 Massachusetts Institute Of Technology Microfluidic-based Gene Synthesis
GB0909660D0 (en) 2009-06-04 2009-07-22 Gene Bridges Gmbh Method of altering nucleic acids
GB0922108D0 (en) 2009-12-17 2010-02-03 Gene Bridges Gmbh Heterologous hosts
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
CN103667331B (zh) * 2013-12-10 2015-09-30 南京师范大学 重组酶基因bet作为一种大肠杆菌异源蛋白表达融合标签的应用
JP6899564B2 (ja) * 2016-08-12 2021-07-07 Nexuspiral株式会社 ゲノム編集方法
CN106995813B (zh) 2017-03-23 2020-06-16 山东大学 基因组大片段直接克隆和dna多分子组装新技术

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE449844T1 (de) * 1997-12-05 2009-12-15 Europ Lab Molekularbiolog Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems
DK1311661T3 (da) * 2000-08-14 2012-11-26 Us Gov Health & Human Serv Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner

Also Published As

Publication number Publication date
JP2004535773A (ja) 2004-12-02
CA2436743A1 (en) 2002-08-15
EP1399546A2 (en) 2004-03-24
WO2002062988A2 (en) 2002-08-15
WO2002062988A3 (en) 2004-01-15
US20040101520A1 (en) 2004-05-27

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