JP4355142B2 - 組換え法 - Google Patents
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- JP4355142B2 JP4355142B2 JP2002563325A JP2002563325A JP4355142B2 JP 4355142 B2 JP4355142 B2 JP 4355142B2 JP 2002563325 A JP2002563325 A JP 2002563325A JP 2002563325 A JP2002563325 A JP 2002563325A JP 4355142 B2 JP4355142 B2 JP 4355142B2
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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| PCT/IB2002/001415 WO2002062988A2 (en) | 2001-02-09 | 2002-02-08 | Recombination method |
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| DK1311661T3 (da) | 2000-08-14 | 2012-11-26 | Us Gov Health & Human Serv | Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner |
| US7521242B2 (en) | 2003-05-09 | 2009-04-21 | The United States Of America As Represented By The Department Of Health And Human Services | Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids |
| US7674621B2 (en) | 2004-05-21 | 2010-03-09 | The United States Of America As Represented By The Department Of Health And Human Services | Plasmids and phages for homologous recombination and methods of use |
| US20070281309A1 (en) * | 2006-05-19 | 2007-12-06 | Massachusetts Institute Of Technology | Microfluidic-based Gene Synthesis |
| GB0909660D0 (en) | 2009-06-04 | 2009-07-22 | Gene Bridges Gmbh | Method of altering nucleic acids |
| GB0922108D0 (en) | 2009-12-17 | 2010-02-03 | Gene Bridges Gmbh | Heterologous hosts |
| GB201009732D0 (en) | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| CN103667331B (zh) * | 2013-12-10 | 2015-09-30 | 南京师范大学 | 重组酶基因bet作为一种大肠杆菌异源蛋白表达融合标签的应用 |
| JP6899564B2 (ja) * | 2016-08-12 | 2021-07-07 | Nexuspiral株式会社 | ゲノム編集方法 |
| CN106995813B (zh) | 2017-03-23 | 2020-06-16 | 山东大学 | 基因组大片段直接克隆和dna多分子组装新技术 |
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| DK1311661T3 (da) * | 2000-08-14 | 2012-11-26 | Us Gov Health & Human Serv | Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner |
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| JP2004535773A (ja) | 2004-12-02 |
| CA2436743A1 (en) | 2002-08-15 |
| EP1399546A2 (en) | 2004-03-24 |
| WO2002062988A2 (en) | 2002-08-15 |
| WO2002062988A3 (en) | 2004-01-15 |
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