JP2004532014A - チトクロームｐ４５０類及びその使用 - Google Patents
チトクロームｐ４５０類及びその使用 Download PDFInfo
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- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
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- C12Y114/13119—5-Epiaristolochene 1,3-dihydroxylase (1.14.13.119)
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| US27442101P | 2001-03-09 | 2001-03-09 | |
| US27559701P | 2001-03-13 | 2001-03-13 | |
| PCT/US2002/006912 WO2002072758A2 (en) | 2001-03-09 | 2002-03-08 | Cytochrome p450s and uses thereof |
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| JP2004532014A true JP2004532014A (ja) | 2004-10-21 |
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| US (7) | US7405057B2 (enExample) |
| EP (1) | EP1373476B1 (enExample) |
| JP (1) | JP2004532014A (enExample) |
| AT (1) | ATE513046T1 (enExample) |
| AU (1) | AU2002244262B2 (enExample) |
| CA (2) | CA2440278C (enExample) |
| WO (1) | WO2002072758A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019524104A (ja) * | 2016-07-20 | 2019-09-05 | フイルメニツヒ ソシエテ アノニムＦｉｒｍｅｎｉｃｈ Ｓａ | ベチバー |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US8106260B2 (en) | 1996-04-12 | 2012-01-31 | The Board Of Trustees Of The University Of Kentucky | Chimeric isoprenoid synthases and uses thereof |
| CA2440278C (en) * | 2001-03-09 | 2017-02-28 | University Of Kentucky Research Foundation | Cytochrome p450s and uses thereof |
| US7812227B2 (en) | 2001-11-13 | 2010-10-12 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
| US10266836B2 (en) | 2001-11-13 | 2019-04-23 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
| US7700834B2 (en) | 2001-11-13 | 2010-04-20 | U.S. Smokless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
| US7700851B2 (en) | 2001-11-13 | 2010-04-20 | U.S. Smokeless Tobacco Company | Tobacco nicotine demethylase genomic clone and uses thereof |
| US8592663B2 (en) | 2001-11-13 | 2013-11-26 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
| US7855318B2 (en) | 2001-11-13 | 2010-12-21 | U.S. Smokeless Tobacco Company | Cloning of cytochrome P450 genes from Nicotiana |
| EP1572911A4 (en) * | 2002-03-12 | 2008-06-25 | Us Smokeless Tobacco Co | CLONING GENES OF CYTOCHROME P450 FROM NICOTIANA |
| EP2336305A3 (en) * | 2002-10-16 | 2011-10-05 | U.S. Smokeless Tobacco Company LLC | Cloning of cytochrome P450 genes from Nicotiana |
| RU2237717C2 (ru) * | 2002-12-20 | 2004-10-10 | Институт биоорганической химии им.академиков М.М.Шемякина и Ю.А.Овчинникова РАН | Рекомбинантная плазмида pgbp450f для получения трансгенных растений и способ получения трансгенных растений табака с повышенной продуктивностью и устойчивостью к грибным фитопатогенам |
| US7442785B2 (en) * | 2003-07-24 | 2008-10-28 | The University Of Kentucky Research Foundation | Sesquiterpene synthase gene and protein |
| US8637731B2 (en) | 2003-10-16 | 2014-01-28 | U.S. Smokeless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
| ZA200602938B (en) | 2003-10-16 | 2008-06-25 | Us Smokeless Tobacco Company | Cloning of cytochrome p450 genes from Nicotiana |
| PL1675957T3 (pl) * | 2003-10-16 | 2012-06-29 | U S Smokeless Tobacco Company Llc | Klonowanie genów cytochromu p450 z Nicotiana |
| US8586837B2 (en) | 2004-04-29 | 2013-11-19 | U.S. Smokeless Tobacco Company Llc | Nicotiana nucleic acid molecules and uses thereof |
| ATE530656T1 (de) | 2005-02-23 | 2011-11-15 | Univ North Carolina State | Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene. |
| SI1919514T1 (sl) | 2005-07-05 | 2012-08-31 | Univ California | Polinukleotidi, ki kodirajo encime, ki modificirajo izoprenoid, in postopki za njihovo uporabo |
| US9370160B2 (en) | 2006-12-15 | 2016-06-21 | Altria Client Services Llc | Tobacco inbred plants ALBEX1F and ALBEX1MS |
| US8319011B2 (en) | 2006-12-15 | 2012-11-27 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| US11332753B2 (en) | 2006-12-15 | 2022-05-17 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| US8124811B2 (en) * | 2007-03-20 | 2012-02-28 | Allylix, Inc. | Fragrance and methods for production of 5-epi-β-vetivone, 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one, and 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-1,6-dien-8-one |
| CL2008001592A1 (es) * | 2007-05-30 | 2009-03-06 | Syngenta Participations Ag | Celula vegetal que comprende una construccion de acido nucleico que contine una secuencia nucleotidica que codifica un citocromo p450, y metodo para conferir resistencia a un herbicida en la planta basado en la transformacion de la construccion descrita anteriormente. |
| US8143039B2 (en) * | 2007-06-01 | 2012-03-27 | Sapphire Energy, Inc. | Use of genetically modified organisms to generate biomass degrading enzymes |
| MX2010002721A (es) * | 2007-09-11 | 2010-06-11 | Sapphire Energy Inc | Metodos para producir productos organicos con organismos fotosinteticos y productos y composiciones de los mismos. |
| EP2188376A4 (en) * | 2007-09-11 | 2013-07-10 | Sapphire Energy Inc | MOLECULAR PRODUCTION BY PHOTOSYNTHETIC ORGANISMS |
| CN101939431B (zh) | 2007-11-12 | 2015-01-28 | 北卡罗来纳州立大学 | 通过修饰特定细胞色素p450基因改变烟草生物碱含量 |
| EP2326707B1 (en) * | 2008-08-12 | 2016-11-23 | Evolva, Inc. | Method for production of isoprenoids |
| CA2786301A1 (en) * | 2010-01-07 | 2011-07-14 | Basf Agro B.V., Arnhem (Nl), Zweigniederlassung Wadenswil | Herbicide-tolerant plants |
| CA2786813C (en) | 2010-01-15 | 2023-03-28 | North Carolina State University | Compositions and methods for minimizing nornicotine synthesis in tobacco |
| EP2680689A1 (en) | 2011-02-28 | 2014-01-08 | North Carolina State University | Tobacco inbred plants ncbex1f, ncbex1ms, and nc ex90 |
| US9550815B2 (en) | 2012-01-23 | 2017-01-24 | University Of British Columbia | ABC terpenoid transporters and methods of using the same |
| EP2943059A1 (en) | 2013-01-11 | 2015-11-18 | North Carolina State University | Tobacco inbred plants k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src and hybrid nc196 src |
| US9560830B2 (en) | 2013-03-05 | 2017-02-07 | North Carolina State University | Tobacco inbred and hybrid plants and uses thereof |
| EP3113602A1 (en) | 2014-03-03 | 2017-01-11 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| EP3113604A1 (en) | 2014-03-03 | 2017-01-11 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| US9596822B2 (en) | 2014-03-03 | 2017-03-21 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| US10774314B2 (en) | 2015-08-21 | 2020-09-15 | Manus Bio Inc. | Increasing productivity of E. coli host cells that functionally express P450 enzymes |
| CN106701696B (zh) * | 2016-12-16 | 2020-02-14 | 江苏省中国科学院植物研究所 | 石蒜科植物忽地笑细胞色素p450还原酶1及其编码基因与应用 |
| CN115058437A (zh) * | 2022-05-23 | 2022-09-16 | 浙江理工大学 | 高效促进丹参酮积累的基因SmCYP82U4及其用途 |
Family Cites Families (22)
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| US5306862A (en) | 1990-10-12 | 1994-04-26 | Amoco Corporation | Method and composition for increasing sterol accumulation in higher plants |
| DE4329969A1 (de) | 1993-09-04 | 1995-03-09 | Basf Ag | Verfahren zur rekombinanten Herstellung von Proteinen in Hefe |
| DE4336810A1 (de) | 1993-10-28 | 1995-05-18 | Basf Ag | Verfahren zur rekombinanten Herstellung von Proteinen in Hefe |
| JP3120684B2 (ja) | 1995-02-14 | 2000-12-25 | トヨタ自動車株式会社 | ゲラニルゲラニル二リン酸を合成する変異型ファルネシル二リン酸合成酵素及びそれをコードするｄｎａ |
| US6100451A (en) | 1995-05-18 | 2000-08-08 | Board Of Trustees Of The University Of Kentucky | Pathogen-inducible regulatory element |
| US5981843A (en) | 1995-05-18 | 1999-11-09 | Board Of Trustee Of The University Of Kentucky | Elicitin-mediated plant resistance |
| FR2738840B1 (fr) | 1995-09-19 | 1997-10-31 | Rhone Poulenc Rorer Sa | Souches de levures genetiquement modifiees |
| ATE363908T1 (de) | 1996-04-09 | 2007-06-15 | Univ Edinburgh | Verwendung von 7-alpha-substituierten steroiden zur behandlung von neuropsychiatrischen erkrankungen |
| US5824774A (en) | 1996-04-12 | 1998-10-20 | Board Of Trustees Of The University Of Kentucky | Chimeric isoprenoid synthases and uses thereof |
| US7186891B1 (en) | 1996-04-12 | 2007-03-06 | University Of Kentucky, Research Foundation | Plant cells and plants expressing chimeric isoprenoid synthases |
| US8106260B2 (en) | 1996-04-12 | 2012-01-31 | The Board Of Trustees Of The University Of Kentucky | Chimeric isoprenoid synthases and uses thereof |
| JP2000508902A (ja) | 1996-04-15 | 2000-07-18 | ワシントン ステイト ユニヴァーシティ リサーチ ファウンデーション | タキソール生合成のための組成物および製造方法 |
| WO1998040505A1 (en) * | 1997-03-13 | 1998-09-17 | Dekalb Genetics Corporation | Maize dimboa biosynthesis genes |
| US6083731A (en) * | 1997-06-24 | 2000-07-04 | Washington State University Research Foundation | Recombinant materials and methods for the production of limonene hydroxylases |
| EP2305825B1 (en) | 1998-07-06 | 2015-01-14 | DCV Inc. doing business as Bio-Technical Resourses | Method of vitamin production |
| AR022383A1 (es) | 1998-09-18 | 2002-09-04 | Univ Kentucky Res Found | Sintasas |
| US6368837B1 (en) * | 1999-08-06 | 2002-04-09 | E. I. Du Pont Nemours And Company | Bioproduction of para-hydroxycinnamic acid |
| CA2440278C (en) | 2001-03-09 | 2017-02-28 | University Of Kentucky Research Foundation | Cytochrome p450s and uses thereof |
| US7442785B2 (en) | 2003-07-24 | 2008-10-28 | The University Of Kentucky Research Foundation | Sesquiterpene synthase gene and protein |
| US8124811B2 (en) | 2007-03-20 | 2012-02-28 | Allylix, Inc. | Fragrance and methods for production of 5-epi-β-vetivone, 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one, and 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-1,6-dien-8-one |
| EP3162787A1 (en) | 2007-03-20 | 2017-05-03 | Evolva, Inc. | Compositions comprising 5-epi-beta-vetivone, and its use as a fragrance |
| EP2326707B1 (en) | 2008-08-12 | 2016-11-23 | Evolva, Inc. | Method for production of isoprenoids |
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2002
- 2002-03-08 CA CA2440278A patent/CA2440278C/en not_active Expired - Lifetime
- 2002-03-08 AT AT02709797T patent/ATE513046T1/de not_active IP Right Cessation
- 2002-03-08 US US10/097,559 patent/US7405057B2/en not_active Expired - Lifetime
- 2002-03-08 EP EP02709797A patent/EP1373476B1/en not_active Expired - Lifetime
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- 2002-03-08 AU AU2002244262A patent/AU2002244262B2/en not_active Ceased
- 2002-03-08 JP JP2002571814A patent/JP2004532014A/ja active Pending
- 2002-03-08 WO PCT/US2002/006912 patent/WO2002072758A2/en not_active Ceased
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- 2013-05-03 US US13/986,446 patent/US8722363B2/en not_active Expired - Fee Related
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- 2014-04-02 US US14/243,778 patent/US20140212939A1/en not_active Abandoned
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019524104A (ja) * | 2016-07-20 | 2019-09-05 | フイルメニツヒ ソシエテ アノニムＦｉｒｍｅｎｉｃｈ Ｓａ | ベチバー |
| JP7026671B2 (ja) | 2016-07-20 | 2022-02-28 | フイルメニツヒ ソシエテ アノニム | ベチバー |
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| CA2440278C (en) | 2017-02-28 |
| US20160032256A1 (en) | 2016-02-04 |
| US20100120110A1 (en) | 2010-05-13 |
| US20030166255A1 (en) | 2003-09-04 |
| US8722363B2 (en) | 2014-05-13 |
| CA2440278A1 (en) | 2002-09-19 |
| US20110318797A1 (en) | 2011-12-29 |
| EP1373476A2 (en) | 2004-01-02 |
| US20140212939A1 (en) | 2014-07-31 |
| EP1373476A4 (en) | 2004-10-27 |
| US20160024530A1 (en) | 2016-01-28 |
| US8263362B2 (en) | 2012-09-11 |
| AU2002244262B2 (en) | 2007-07-12 |
| US10184118B2 (en) | 2019-01-22 |
| WO2002072758A2 (en) | 2002-09-19 |
| WO2002072758A3 (en) | 2003-10-30 |
| CA2954889A1 (en) | 2002-09-19 |
| US8445231B2 (en) | 2013-05-21 |
| US7405057B2 (en) | 2008-07-29 |
| EP1373476B1 (en) | 2011-06-15 |
| US20130330793A1 (en) | 2013-12-12 |
| US10184119B2 (en) | 2019-01-22 |
| ATE513046T1 (de) | 2011-07-15 |
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