AU2002244262B2 - Cytochrome P450s and uses thereof - Google Patents

Cytochrome P450s and uses thereof Download PDF

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Publication number
AU2002244262B2
AU2002244262B2 AU2002244262A AU2002244262A AU2002244262B2 AU 2002244262 B2 AU2002244262 B2 AU 2002244262B2 AU 2002244262 A AU2002244262 A AU 2002244262A AU 2002244262 A AU2002244262 A AU 2002244262A AU 2002244262 B2 AU2002244262 B2 AU 2002244262B2
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AU
Australia
Prior art keywords
plant
cell
polypeptide
seq
cytochrome
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Ceased
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AU2002244262A
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AU2002244262A1 (en
Inventor
Joseph Chappell
Lyle F. Ralston
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University of Kentucky Research Foundation
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University of Kentucky Research Foundation
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Publication of AU2002244262B2 publication Critical patent/AU2002244262B2/en
Priority to AU2007221982A priority Critical patent/AU2007221982A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13078Ent-kaurene oxidase (1.14.13.78)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/131195-Epiaristolochene 1,3-dihydroxylase (1.14.13.119)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2002244262A 2001-03-09 2002-03-08 Cytochrome P450s and uses thereof Ceased AU2002244262B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2007221982A AU2007221982A1 (en) 2001-03-09 2007-10-12 Cytochrome P450S and uses thereof

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US27442101P 2001-03-09 2001-03-09
US60/274,421 2001-03-09
US27559701P 2001-03-13 2001-03-13
US60/275,597 2001-03-13
PCT/US2002/006912 WO2002072758A2 (en) 2001-03-09 2002-03-08 Cytochrome p450s and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2007221982A Division AU2007221982A1 (en) 2001-03-09 2007-10-12 Cytochrome P450S and uses thereof

Publications (2)

Publication Number Publication Date
AU2002244262A1 AU2002244262A1 (en) 2003-03-20
AU2002244262B2 true AU2002244262B2 (en) 2007-07-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002244262A Ceased AU2002244262B2 (en) 2001-03-09 2002-03-08 Cytochrome P450s and uses thereof

Country Status (7)

Country Link
US (7) US7405057B2 (enExample)
EP (1) EP1373476B1 (enExample)
JP (1) JP2004532014A (enExample)
AT (1) ATE513046T1 (enExample)
AU (1) AU2002244262B2 (enExample)
CA (2) CA2440278C (enExample)
WO (1) WO2002072758A2 (enExample)

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US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
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US7700851B2 (en) 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
EP1572911A4 (en) * 2002-03-12 2008-06-25 Us Smokeless Tobacco Co CLONING GENES OF CYTOCHROME P450 FROM NICOTIANA
EP2336305A3 (en) * 2002-10-16 2011-10-05 U.S. Smokeless Tobacco Company LLC Cloning of cytochrome P450 genes from Nicotiana
RU2237717C2 (ru) * 2002-12-20 2004-10-10 Институт биоорганической химии им.академиков М.М.Шемякина и Ю.А.Овчинникова РАН Рекомбинантная плазмида pgbp450f для получения трансгенных растений и способ получения трансгенных растений табака с повышенной продуктивностью и устойчивостью к грибным фитопатогенам
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ZA200602938B (en) 2003-10-16 2008-06-25 Us Smokeless Tobacco Company Cloning of cytochrome p450 genes from Nicotiana
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US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
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SI1919514T1 (sl) 2005-07-05 2012-08-31 Univ California Polinukleotidi, ki kodirajo encime, ki modificirajo izoprenoid, in postopki za njihovo uporabo
US9370160B2 (en) 2006-12-15 2016-06-21 Altria Client Services Llc Tobacco inbred plants ALBEX1F and ALBEX1MS
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8124811B2 (en) * 2007-03-20 2012-02-28 Allylix, Inc. Fragrance and methods for production of 5-epi-β-vetivone, 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one, and 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-1,6-dien-8-one
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EP2188376A4 (en) * 2007-09-11 2013-07-10 Sapphire Energy Inc MOLECULAR PRODUCTION BY PHOTOSYNTHETIC ORGANISMS
CN101939431B (zh) 2007-11-12 2015-01-28 北卡罗来纳州立大学 通过修饰特定细胞色素p450基因改变烟草生物碱含量
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Also Published As

Publication number Publication date
CA2440278C (en) 2017-02-28
US20160032256A1 (en) 2016-02-04
US20100120110A1 (en) 2010-05-13
US20030166255A1 (en) 2003-09-04
JP2004532014A (ja) 2004-10-21
US8722363B2 (en) 2014-05-13
CA2440278A1 (en) 2002-09-19
US20110318797A1 (en) 2011-12-29
EP1373476A2 (en) 2004-01-02
US20140212939A1 (en) 2014-07-31
EP1373476A4 (en) 2004-10-27
US20160024530A1 (en) 2016-01-28
US8263362B2 (en) 2012-09-11
US10184118B2 (en) 2019-01-22
WO2002072758A2 (en) 2002-09-19
WO2002072758A3 (en) 2003-10-30
CA2954889A1 (en) 2002-09-19
US8445231B2 (en) 2013-05-21
US7405057B2 (en) 2008-07-29
EP1373476B1 (en) 2011-06-15
US20130330793A1 (en) 2013-12-12
US10184119B2 (en) 2019-01-22
ATE513046T1 (de) 2011-07-15

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