JP2004505988A5 - - Google Patents
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- Publication number
- JP2004505988A5 JP2004505988A5 JP2002518237A JP2002518237A JP2004505988A5 JP 2004505988 A5 JP2004505988 A5 JP 2004505988A5 JP 2002518237 A JP2002518237 A JP 2002518237A JP 2002518237 A JP2002518237 A JP 2002518237A JP 2004505988 A5 JP2004505988 A5 JP 2004505988A5
- Authority
- JP
- Japan
- Prior art keywords
- group
- alkyl
- nucleic acid
- aryl
- binding compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108020004707 nucleic acids Proteins 0.000 claims 87
- 102000039446 nucleic acids Human genes 0.000 claims 87
- 150000007523 nucleic acids Chemical class 0.000 claims 86
- 238000009739 binding Methods 0.000 claims 72
- 150000001875 compounds Chemical class 0.000 claims 69
- 125000000008 (C1-C10) alkyl group Chemical group 0.000 claims 41
- 125000004169 (C1-C6) alkyl group Chemical group 0.000 claims 29
- 125000006853 reporter group Chemical group 0.000 claims 26
- 229910052736 halogen Inorganic materials 0.000 claims 21
- 238000000034 method Methods 0.000 claims 18
- 125000003118 aryl group Chemical group 0.000 claims 16
- 125000000217 alkyl group Chemical group 0.000 claims 15
- 125000006374 C2-C10 alkenyl group Chemical group 0.000 claims 14
- 125000000623 heterocyclic group Chemical group 0.000 claims 13
- 125000001424 substituent group Chemical group 0.000 claims 12
- 125000000304 alkynyl group Chemical group 0.000 claims 11
- 125000006239 protecting group Chemical group 0.000 claims 11
- 125000006375 C2-C10 alkynyl group Chemical group 0.000 claims 10
- 125000003342 alkenyl group Chemical group 0.000 claims 10
- 230000000295 complement effect Effects 0.000 claims 10
- 238000009396 hybridization Methods 0.000 claims 10
- -1 nucleic acid compound Chemical class 0.000 claims 10
- 239000000203 mixture Substances 0.000 claims 8
- 150000003839 salts Chemical class 0.000 claims 7
- QUKPALAWEPMWOS-UHFFFAOYSA-N 1h-pyrazolo[3,4-d]pyrimidine Chemical compound C1=NC=C2C=NNC2=N1 QUKPALAWEPMWOS-UHFFFAOYSA-N 0.000 claims 6
- 125000004093 cyano group Chemical group *C#N 0.000 claims 6
- 229910052760 oxygen Inorganic materials 0.000 claims 6
- 229910052717 sulfur Inorganic materials 0.000 claims 6
- 238000010521 absorption reaction Methods 0.000 claims 5
- 239000007850 fluorescent dye Substances 0.000 claims 5
- 239000007790 solid phase Substances 0.000 claims 5
- 125000004178 (C1-C4) alkyl group Chemical group 0.000 claims 4
- 125000006569 (C5-C6) heterocyclic group Chemical group 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims 4
- 239000003795 chemical substances by application Substances 0.000 claims 4
- 238000003776 cleavage reaction Methods 0.000 claims 4
- 238000004132 cross linking Methods 0.000 claims 4
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims 4
- 230000003834 intracellular effect Effects 0.000 claims 4
- 238000010791 quenching Methods 0.000 claims 4
- 230000000171 quenching effect Effects 0.000 claims 4
- 230000007017 scission Effects 0.000 claims 4
- 125000000464 thioxo group Chemical group S=* 0.000 claims 4
- 229910052757 nitrogen Inorganic materials 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 239000007787 solid Substances 0.000 claims 3
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical class NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 2
- 229930024421 Adenine Natural products 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 2
- 229960000643 adenine Drugs 0.000 claims 2
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 230000037452 priming Effects 0.000 claims 2
- ZXYTVHYAIWRRCA-UHFFFAOYSA-N 2-amino-1,7-dihydropurin-6-one 1H-pyrazolo[3,4-d]pyrimidine Chemical class C1=NC=C2C=NNC2=N1.N1C(N)=NC(=O)C2=C1N=CN2 ZXYTVHYAIWRRCA-UHFFFAOYSA-N 0.000 claims 1
- JJTNLWSCFYERCK-UHFFFAOYSA-N 7h-pyrrolo[2,3-d]pyrimidine Chemical compound N1=CN=C2NC=CC2=C1 JJTNLWSCFYERCK-UHFFFAOYSA-N 0.000 claims 1
- 125000006725 C1-C10 alkenyl group Chemical group 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 claims 1
- 125000006323 alkenyl amino group Chemical group 0.000 claims 1
- 125000006319 alkynyl amino group Chemical group 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 239000001177 diphosphate Substances 0.000 claims 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims 1
- 235000011180 diphosphates Nutrition 0.000 claims 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 230000007062 hydrolysis Effects 0.000 claims 1
- 238000006460 hydrolysis reaction Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000002844 melting Methods 0.000 claims 1
- 230000008018 melting Effects 0.000 claims 1
- 125000000449 nitro group Chemical class [O-][N+](*)=O 0.000 claims 1
- 239000012071 phase Substances 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
- 239000005373 porous glass Substances 0.000 claims 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims 1
- 239000000377 silicon dioxide Substances 0.000 claims 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims 1
- 125000002264 triphosphate group Chemical group [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP00116816 | 2000-08-03 | ||
| EP01109438 | 2001-04-24 | ||
| PCT/EP2001/008850 WO2002012263A1 (en) | 2000-08-03 | 2001-07-31 | Nucleic acid binding compounds containing pyrazolo[3,4-d]pyrimidine analogues of purin-2,6-diamine and their uses |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004505988A JP2004505988A (ja) | 2004-02-26 |
| JP2004505988A5 true JP2004505988A5 (https=) | 2005-02-24 |
| JP4331476B2 JP4331476B2 (ja) | 2009-09-16 |
Family
ID=26071249
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002518237A Expired - Lifetime JP4331476B2 (ja) | 2000-08-03 | 2001-07-31 | プリン−２，６−ジアミンのピラゾロ［３，４−ｄ］ピリミジンアナローグを含有する核酸結合性化合物およびそれらの使用 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US7238795B2 (https=) |
| EP (1) | EP1307469B1 (https=) |
| JP (1) | JP4331476B2 (https=) |
| AT (1) | ATE384731T1 (https=) |
| AU (1) | AU2001278517A1 (https=) |
| CA (1) | CA2416631C (https=) |
| DE (1) | DE60132596T2 (https=) |
| ES (1) | ES2300346T3 (https=) |
| WO (1) | WO2002012263A1 (https=) |
Families Citing this family (72)
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| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
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| US5801155A (en) * | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
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| US6949367B1 (en) * | 1998-04-03 | 2005-09-27 | Epoch Pharmaceuticals, Inc. | Modified oligonucleotides for mismatch discrimination |
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| US7715989B2 (en) * | 1998-04-03 | 2010-05-11 | Elitech Holding B.V. | Systems and methods for predicting oligonucleotide melting temperature (TmS) |
| US6683173B2 (en) * | 1998-04-03 | 2004-01-27 | Epoch Biosciences, Inc. | Tm leveling methods |
| US7045610B2 (en) * | 1998-04-03 | 2006-05-16 | Epoch Biosciences, Inc. | Modified oligonucleotides for mismatch discrimination |
| DE19823454A1 (de) * | 1998-05-18 | 1999-11-25 | Epigenomics Gmbh | Verfahren zur photolithographischen Herstellung von DNA, PNA und Protein Chips |
| EP1261616B1 (en) | 2000-03-01 | 2010-08-18 | Epoch Biosciences, Inc. | Modified oligonucleotides for mismatch discrimination |
| KR20090009660A (ko) | 2007-07-20 | 2009-01-23 | 주식회사 엘지화학 | 표면 개질 아민계 경화제와 이를 포함하는 일액성 에폭시수시 조성물 및 이방 도전성 접착 재료 |
-
2001
- 2001-07-31 WO PCT/EP2001/008850 patent/WO2002012263A1/en not_active Ceased
- 2001-07-31 ES ES01956576T patent/ES2300346T3/es not_active Expired - Lifetime
- 2001-07-31 AT AT01956576T patent/ATE384731T1/de active
- 2001-07-31 EP EP01956576A patent/EP1307469B1/en not_active Expired - Lifetime
- 2001-07-31 AU AU2001278517A patent/AU2001278517A1/en not_active Abandoned
- 2001-07-31 DE DE60132596T patent/DE60132596T2/de not_active Expired - Lifetime
- 2001-07-31 CA CA2416631A patent/CA2416631C/en not_active Expired - Lifetime
- 2001-07-31 JP JP2002518237A patent/JP4331476B2/ja not_active Expired - Lifetime
- 2001-07-31 US US10/333,518 patent/US7238795B2/en not_active Expired - Lifetime
-
2007
- 2007-04-30 US US11/742,416 patent/US8057997B2/en not_active Expired - Lifetime
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