JP2004137168A - Skin care preparation for external use, cell activator and antioxidant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation for external use exhibiting excellent effects on prevention and amelioration of aging symptoms of the skin, and to obtain a cell activator having the excellent effects and an antioxidant having the excellent effects. <P>SOLUTION: An extract from one or two or more species of plants of the genus Sesuvium is formulated in the skin care preparation for external use in order to prevent and ameliorate the aging symptoms of the skin. Furthermore, the extract from the one or two or more species of plants of the genus Sesuvium is used as the cell activator and antioxidant. <P>COPYRIGHT: (C)2004,JPO

Description

【００２３】
表１より明らかなように、ミルスベリヒユ抽出物を添加した培地では、有意な真皮線維芽細胞賦活作用が認められた。特に、ミルスベリヒユ抽出物を０．０８〜１０．０ｍｇ／ｍＬ添加した場合に、ブランクと比較して、危険率１％未満で有意な真皮線維芽細胞賦活作用が認められた。このことから、ミルスベリヒユ抽出物は、優れた真皮線維芽細胞賦活作用を有することが明らかとなった。
【００２４】
次に、セスビウム属（Ｓｅｓｕｖｉｕｍ）植物抽出物の抗酸化作用について示す。試料には、ミルスベリヒユ（Ｓｅｓｕｖｉｕｍ　ｐｏｒｔｕｌａｃａｓｔｒｕｍ）の全草より調製方法１を用いて抽出したミルスベリヒユ抽出物を用いた。
【００２５】
評価は、以下の手順で行った。５０重量％エタノール水溶液にて１０ｍｇ／ｍＬに希釈したミルスベリヒユ抽出物溶液を９６穴マイクロプレートに１００μＬ添加した。次に、０．２ｍＭの濃度になるようにエタノールにて調製した１，１−ジフェニル−２−ピクリルヒドラジル（ＤＰＰＨ）溶液を９６穴マイクロプレートに１００μＬ添加した。攪拌しながら暗所に放置し、１０分後と２４時間後に５１６ｎｍの吸光度を測定した。試料が無添加のブランクの吸光度を（Ａ）、試料を添加したときの吸光度を（Ｂ）としたとき、式（１）の値をラジカル消去率とした。
式（１）　｛１−（Ｂ）／（Ａ）｝×１００（％）
ミルスベリヒユ抽出物の実験結果を表２に示す。
【００２６】
【表２】

【００２７】
表２より明らかなように、ミルスベリヒユ抽出物は優れた抗酸化作用を有することが分かった。
【００２８】
続いて、本発明に係るセスビウム属（Ｓｅｓｕｖｉｕｍ）植物抽出物を配合した皮膚外用剤の処方を示す。実施例１〜５の処方には、表３に示すセスビウム属（Ｓｅｓｕｖｉｕｍ）植物抽出物を配合し、実施例６〜１６の各処方には、それぞれの処方に記載のセスビウム属（Ｓｅｓｕｖｉｕｍ）植物の全草から得られる抽出物を配合した。
【００２９】

製法：（１）〜（６）の油相成分を８０℃にて加熱溶解する。一方（７）〜（１０）の水相成分を８０℃にて加熱溶解する。これに前記油相成分を攪拌しながら加え、ホモジナイザーにより均一に乳化する。乳化終了後、冷却を開始し、（１１）と（１２）を順次加え、均一に混合する。
【００３０】
【表３】

【００３１】

製法：（１）に（２）及び（３）を溶解する。溶解後、（４）〜（８）を順次添加した後、十分に攪拌し、（９）を加え、均一に混合する。
【００３２】

製法：（１）〜（６）の油相成分を８０℃にて加熱溶解する。一方（７）〜（１０）の水相成分を８０℃にて加熱溶解する。これに前記油相成分を攪拌しながら加え、ホモジナイザーにより均一に乳化する。乳化終了後、（１１）を加え、冷却を開始し、４０℃にて（１２）を加え、均一に混合する。
【００３３】

製法：（１）〜（６）の水相成分を混合し、７５℃にて加熱溶解する。一方、（７）〜（１４）の油相成分を混合し、７５℃にて加熱溶解する。次いで、上記水相成分に油相成分を添加して予備乳化を行った後、ホモミキサーにて均一に乳化する。乳化終了後に冷却を開始し、５０℃にて（１５）を加える。さらに４０℃まで冷却し、（１６）を加え、均一に混合する。
【００３４】

製法：（１）を（２）に加え、均一に攪拌した後、（３）を加える。均一に攪拌した後，（４）に予め溶解した（５）を加える。均一に攪拌した後、予め混合しておいた（６）〜（８）を加える。均一に攪拌した後、（９）〜（１０）を順次加え、均一に混合する。
【００３５】

製法：（１）と（２）を均一に溶解する。これに、（３）〜（５）を順次加え、均一に混合する。
【００３６】

製法：（１）〜（４）の油相成分を８０℃にて加熱溶解する。一方（５）〜（７）の水相成分を８０℃にて加熱溶解し、油相成分と均一に混合撹拌する。冷却を開始し、４０℃にて（８）と（９）を加え、均一に混合する。
【００３７】

製法：（１）〜（４）の油相成分を混合し、７５℃にて加熱溶解する。一方、（５）〜（７）の水相成分を混合し、７５℃にて加熱溶解し、これに（８）〜（１０）の顔料を加え、ホモミキサーにて均一に分散させる。この水相成分に前記油相成分を加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、４０℃にて（１１）〜（１３）の成分を加え、均一に混合する。
【００３８】

製法：（１）〜（６）の油相成分を混合し、７５℃にて加熱溶解する。一方、（７）〜（１０）の水相成分を混合し、７５℃にて加熱溶解し、これに（１１）〜（１５）の顔料を加え、ホモミキサーにて均一に分散する。油相成分を加え、乳化を行う。乳化終了後に冷却を開始し、４０℃にて（１６）〜（１８）の成分を順次加え、均一に混合する。
【００３９】

製法：（５）と（６）を（１１）の一部に溶解して５０℃とし、５０℃に加熱した（４）に撹拌しながら徐々に加える。これを混合した後、７０℃にて加熱溶解した（１）〜（３）に均一に分散する。これに（７）〜（１０）を（１１）の残部に７０℃にて加熱溶解したものを撹拌しながら加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、４０℃にて（１２）と（１３）を加え、均一に混合する。
【００４０】

製法：（２）と（３）を混合し、８０℃に加温した後、８０℃に加温した（１）に溶解する。均一に溶解した後、（４）〜（５）を加え、攪拌しながら冷却を開始する。４０℃まで冷却し、（６）〜（８）を加え、均一に混合する。
【００４１】

製法：（１）〜（５）を均一に混合する。
【００４２】
本発明の実施例１〜５について使用試験を行い、シワ，タルミ，肌のハリの改善効果を評価した。その際、実施例１において、配合したミルスベリヒユ抽出物を精製水に代替し、比較例１として同時に使用試験を行った。
【００４３】
各試料について、シワ，タルミ，肌のハリの低下といった症状が顕著に認められる５０〜６０才代の男女パネラー各２０名を一群とし、ブラインドにて１カ月間使用させ、使用前後の皮膚状態の変化を観察して評価した。皮膚症状の指標として、シワ，タルミ，肌のハリについて、「改善」，「やや改善」，「変化なし」の三段階で評価し、表４に各評価を得たパネラー数にて示した。
【００４４】
【表４】

【００４５】
表４より、シワ，タルミ，肌のハリについて、セスビウム属（Ｓｅｓｕｖｉｕｍ）植物抽出物を含有しない比較例使用群においては、半数以上のパネラーに改善は認められなかったが、セスビウム属（Ｓｅｓｕｖｉｕｍ）植物抽出物を配合した実施例使用群においては、６割以上のパネラーに明確な改善が認められた。
【００４６】
以上のように、本発明の実施例においては、従来の比較例よりも、シワ，タルミ，肌のハリといった老化症状の改善に優れた効果を有していた。
【００４７】
【発明の効果】
以上詳述したように、本発明により、皮膚の老化症状の防止・改善に優れた効果を有する皮膚外用剤、優れた効果を有する細胞賦活剤、及び優れた効果を有する抗酸化剤を得ることが出来た。[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to an external preparation for skin, a cell activator, and an antioxidant that exhibit excellent effects for preventing and improving aging symptoms. More particularly, the skin external preparation containing Sesubiumu genus (Sesuvium) plant extracts, and Sesubiumu genus (Sesuvium) cell activator as an active ingredient a plant extract, and Sesubiumu genus (Sesuvium) plant extract as an active ingredient Related to antioxidants.
[0002]
[Prior art]
A decrease in the function of dermal fibroblasts due to aging or the like causes a decrease or degeneration of the dermal matrix such as collagen or elastin, and is an important factor in aging symptoms such as wrinkles and decreased elasticity of the skin. In addition, oxidative damage due to external stress such as ultraviolet rays also causes aging symptoms such as wrinkles, spots, and decreased elasticity of the skin. In the field of skin external preparations so far, various cell activators and antioxidants have been searched for and formulated to prevent or ameliorate the aging symptoms caused by such cell function deterioration and oxidative damage. As the cell activator, the essence of Ponkan (see Patent Document 1), Trichoderma genus, smelt and their extracts (see Patent Document 2), and a chlorella extract with an organic solvent (see Patent Document 3) are known. As an antioxidant, an extract of a plant belonging to the genus Heteroteka of the Compositae family (see Patent Document 4) and an extract of a plant belonging to the genus Sargassum in the family Asteraceae are known (see Patent Document 5).
[0003]
As the prior art Sesubiumu genus (Sesuvium) plants according to the present invention, that the Agrobacterium was allowed to infected tissue culture into Sesubiumu genus plant extracts Fight Aix die steroid from the resulting culture A method for producing a phytoecdysteroid which is characteristic (see Patent Document 6) and the use of a plant of the genus Cesbium as an insect molting hormone high producing plant (see Patent Document 7) are disclosed. However, none of these known inventions discloses any use of a Cesbium plant extract for skin external preparations, cell activators, and antioxidants.
[0004]
[Patent Document 1]
Japanese Patent Application Laid-Open No. 2001-131455 [Patent Document 2]
JP 2000-178198 A [Patent Document 3]
JP-A-11-335293 [Patent Document 4]
JP-A-11-180886 [Patent Document 5]
JP-A-10-182413 [Patent Document 6]
JP-A-1-31797 [Patent Document 7]
JP-A-4-197118 [0005]
[Problems to be solved by the invention]
Conventionally used cell activators and antioxidants often act only in a part of the aging phenomenon, and it is considered that the effect is essentially insufficient as an aging improvement effect. In addition, when formulated in a base of a skin external preparation, to obtain an effective effect, a considerably high concentration must be blended, which may give an undesirable color or odor to the formulation, At present, there are few things that can satisfy all of the effects and stability. For this reason, development of a better active ingredient is expected, and the present invention has been made in view of such circumstances. Accordingly, an object of the present invention is to provide a skin external preparation that exhibits an excellent effect of preventing and improving aging symptoms of the skin, a cell activator having an excellent effect, and an antioxidant having an excellent effect. is there.
[0006]
[Means for Solving the Problems]
The present inventors have conducted studies on cell activating and antioxidant effects of various substances in order to find components excellent in preventing and improving aging symptoms of the skin. As a result, the present inventors have found excellent cell activating and antioxidant effects in a Sesuvium plant extract, and have conducted further studies to complete the present invention.
[0007]
BEST MODE FOR CARRYING OUT THE INVENTION
Plants used as a raw material of the present invention may be any plant of Sesubiumu genus aizoaceae (Aizoaceae) (Sesuvium). The Sesubiumu genus (Sesuvium) plant, mill purslane (Sesuvium portulacastrum), Sesubiumu Erekutsumu (Sesuvium erec tum), Sesubiumu tri Ante Moi des (Sesuvium trianthemoides), Sesubiumu Seshire (Sesuvium sessile), Sesubiumu Berukosumu (Sesuvium verrucosum), Sesubiumu Marichimamu ( Sesuvium maritimum), such as Seth bi Umm Seth Bioi Death (Sesuvium sesuvioides) it is known. Among these, as appropriate as a raw material for reasons such as availability is relatively easy, mill purslane (Sesuvium portulacastrum), Sesubiumu Marichimamu (Sesuvium maritimum), and the like.
[0008]
When using these Sesubiumu genera (Sesuvium) plants, use the extract is generally used. The extraction, the Sesubiumu genus (Sesuvium) plant stems, leaves, flowers, seeds, roots, but may be used any part of such shoots, conveniently utilized, may be used whole herbs. At the time of extraction, it may be used as it is, but in consideration of extraction efficiency, it is preferable to perform extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersion in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, homogenization may be performed with stirring or in an extraction solvent. It is appropriate to set the extraction temperature at a temperature of about 5 ° C. to the boiling point of the extraction solvent or lower. The extraction time varies depending on the type of the extraction solvent and the extraction temperature, but is suitably about 1 hour to 14 days.
[0009]
Examples of the extraction solvent include water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, and ethers such as ethyl ether and propyl ether. Solvents, esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or two or more solvents are selected from these solvents. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Further, one or more supercritical fluids or subcritical fluids such as water, carbon dioxide, ethylene, propylene, ethanol, methanol, and ammonia may be used.
[0010]
Sesubiumu genus (Sesuvium) extracts according to the above solvent plants, can be used it is, concentrated, what dryness or redissolved in water or a polar solvent, or in a range that does not impair these physiological effects It may be used after performing purification treatment such as decolorization, deodorization, and desalting, or after performing fractionation treatment by column chromatography or the like. The extract of the genus Sesuvium , its processed product and the fractionated product may be freeze-dried after each treatment and fractionation, and may be used by dissolving in a solvent at the time of use. It can also be used by being encapsulated in vesicles such as liposomes or microcapsules.
[0011]
The external preparation for skin which exerts an excellent effect on prevention and improvement of aging symptoms in the present invention contains the above-described Sesuvium plant extract. Also, cell activator and antioxidant exhibits excellent effects as an active ingredient Sesubiumu genus (Sesuvium) plant extract according.
[0012]
The amount of Sesubiumu genus (Sesuvium) plant extracts in the present invention can be adjusted depending on the type and purpose and the like of the external preparation for skin, based on the total amount of the external preparation for skin, from 0.0001 to 10.0% by weight Is more preferable, and more preferably 0.001 to 5.0% by weight.
[0013]
The external preparation for skin according to the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders, granules and the like.
[0014]
Note that the external skin preparation according to the present invention, in addition to Sesubiumu genera (Sesuvium) plant extracts, usually pharmaceuticals, quasi drugs, skin cosmetics are formulated hair cosmetic and cleansing compositions, the oil component , Humectants, powders, pigments, emulsifiers, solubilizers, detergents, ultraviolet absorbers, thickeners, drugs, fragrances, resins, antibacterial and fungicides, alcohols and the like can be appropriately compounded. Further, as long as the effects of the present invention are not impaired, it can be used in combination with other cell activators, antioxidants, and plant extracts.
[0015]
【Example】
Further, the features of the present invention will be described in detail with reference to examples. First, a method for preparing a Sesuvium plant extract of the present invention will be described.
[0016]
[Preparation method 1]
10 kg of a 50% by weight aqueous ethanol solution was added to 1 kg of a dry and crushed whole plant of the genus Sesuvium and immersed at room temperature for 7 days. The extract was collected by filtration, and after removing the solvent, an extract of Sesuvium plant was obtained.
[0017]
[Preparation method 2]
9 liters of water was added to 1 kg of the dried and ground material of the whole plant of the genus Sesuvium, and the mixture was extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of Sesuvium plant was obtained.
[0018]
[Preparation method 3]
9 liters of methanol was added to 1 kg of a dry and ground product of the whole plant of the genus Sesuvium and immersed at room temperature for 7 days. The extract was collected by filtration, and after removing the solvent, an extract of Sesuvium plant was obtained.
[0019]
[Preparation method 4]
The whole plant of the genus Sesuvium was put into a supercritical extraction apparatus, and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was recovered to obtain a Sesuvium plant extract.
[0020]
Next, the dermal fibroblast activating effect of a Sesuvium plant extract will be described. As a sample, a Millsbergi extract extracted from the whole plant of Sesuvium portulaca strum using Preparation Method 1 was used.
[0021]
The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle's medium (DMEM) supplemented with 1% fetal bovine serum. After culturing for 24 hours, the medium was replaced with a test medium to which a sample of an arbitrary concentration was added, followed by culturing for another 48 hours. Then, the medium was replaced with a medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) and cultured for 2 hours to remove formazan generated by opening the tetrazolium ring. Extraction was performed with 2-propanol, and the absorbance at 550 nm was measured using a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation was evaluated by the difference between the two measured values. In addition, in order to confirm the validity of the measurement method, Dulbecco's modified Eagle medium (DMEM) supplemented with 5% fetal bovine serum was used as a positive control instead of the medium supplemented with the sample, and the measurement was performed. The evaluation results are shown in Table 1 as relative values with the cell activating effect in the blank where no sample was added as 100.
[0022]
[Table 1]

[0023]
As is evident from Table 1, a significant effect of dermal fibroblast activation was observed in the medium to which the Milles versicolor extract was added. In particular, when 0.08 to 10.0 mg / mL of the Millsberghis extract was added, a significant dermal fibroblast activating effect was observed at a risk factor of less than 1% as compared with the blank. From this, it became clear that the Myrsbergenis extract had an excellent dermal fibroblast activating action.
[0024]
Next, the antioxidant activity of the Sesuvium plant extract will be described. As a sample, an extract of Milles versicolor extracted from the whole plant of Sesuvium portulacastrum using Preparation Method 1 was used.
[0025]
The evaluation was performed according to the following procedure. 100 μL of a Milles versicolor extract solution diluted to 10 mg / mL with a 50% by weight aqueous ethanol solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was allowed to stand in a dark place with stirring, and the absorbance at 516 nm was measured after 10 minutes and 24 hours. When the absorbance of a blank where no sample was added was (A) and the absorbance when the sample was added was (B), the value of equation (1) was defined as the radical scavenging rate.
Formula (1) {1- (B) / (A)} × 100 (%)
Table 2 shows the experimental results of the Millisbergi extract.
[0026]
[Table 2]

[0027]
As is clear from Table 2, it was found that the Myrsbergeniflora extract had an excellent antioxidant effect.
[0028]
Next, the formulation of an external preparation for skin containing the Sesuvium plant extract according to the present invention will be described. The formulation of Examples 1 to 5, blended Sesubiumu genus (Sesuvium) plant extracts shown in Table 3, each formulation of Examples 6-16, Sesubiumu genus according to each formulation (Sesuvium) Plant The extract obtained from the whole plant was blended.
[0029]

Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added thereto with stirring, and the mixture is uniformly emulsified by a homogenizer. After the emulsification is completed, cooling is started, (11) and (12) are sequentially added, and mixed uniformly.
[0030]
[Table 3]

[0031]

Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, followed by sufficient stirring, and (9) is added, followed by uniform mixing.
[0032]

Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added thereto with stirring, and the mixture is uniformly emulsified by a homogenizer. After the emulsification is completed, (11) is added, cooling is started, (12) is added at 40 ° C., and the mixture is uniformly mixed.
[0033]

Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and heated and dissolved at 75 ° C. Next, after preliminarily emulsifying by adding an oil phase component to the above water phase component, the mixture is uniformly emulsified by a homomixer. After completion of the emulsification, cooling is started, and (15) is added at 50 ° C. Further cool to 40 ° C., add (16) and mix uniformly.
[0034]

Production method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, (6) to (8), which have been mixed in advance, are added. After stirring uniformly, (9) to (10) are sequentially added and uniformly mixed.
[0035]

Production method: (1) and (2) are uniformly dissolved. To this, (3) to (5) are sequentially added and uniformly mixed.
[0036]

Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and uniformly mixed and stirred with the oil phase component. Start cooling, add (8) and (9) at 40 ° C and mix uniformly.
[0037]

Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and uniformly dispersed by a homomixer. The oil phase component is added to the water phase component and emulsified by a homomixer. After the completion of the emulsification, cooling is started, and the components (11) to (13) are added at 40 ° C. and mixed uniformly.
[0038]

Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed by a homomixer. Add oil phase components and emulsify. After completion of the emulsification, cooling is started, and the components (16) to (18) are sequentially added at 40 ° C. and uniformly mixed.
[0039]

Production method: (5) and (6) are dissolved in a part of (11) to 50 ° C., and gradually added to (4) heated to 50 ° C. with stirring. After mixing, the mixture is heated and dissolved at 70 ° C., and is uniformly dispersed in (1) to (3). A solution obtained by heating and dissolving (7) to (10) in the remainder of (11) at 70 ° C. is added to the mixture with stirring, and the mixture is emulsified by a homomixer. After completion of the emulsification, cooling is started, and (12) and (13) are added at 40 ° C. and mixed uniformly.
[0040]

Production method: (2) and (3) are mixed, heated to 80 ° C, and dissolved in (1) heated to 80 ° C. After uniform dissolution, (4) and (5) are added, and cooling is started with stirring. Cool to 40 ° C., add (6) to (8), and mix uniformly.
[0041]

Production method: (1) to (5) are uniformly mixed.
[0042]
A use test was performed on Examples 1 to 5 of the present invention to evaluate the effect of improving wrinkles, talmy, and skin firmness. At that time, in Example 1, the blended Milles versicolor extract was replaced with purified water, and a use test was performed simultaneously as Comparative Example 1.
[0043]
For each sample, a group of 20 male and female panelists in their 50s and 60s, each with noticeable symptoms such as wrinkles, tarmi, and reduced skin firmness, was used as a group for one month using blinds, and the skin condition before and after use was examined. The change was observed and evaluated. As indicators of skin symptoms, wrinkles, tarmi, and skin firmness were evaluated in three stages of “improvement”, “slight improvement”, and “no change”, and Table 4 shows the number of panelists who obtained each evaluation.
[0044]
[Table 4]

[0045]
From Table 4, wrinkles, sagging, the elasticity of the skin, in the comparative example using a group containing no Sesubiumu genus (Sesuvium) plant extracts, although improvement in more than half of the panelists were not observed, Sesubiumu genus (Sesuvium) Plants In the group using the example in which the extract was blended, a clear improvement was recognized in 60% or more of the panelists.
[0046]
As described above, in the examples of the present invention, the effect of improving the aging symptoms such as wrinkles, tarmi, and skin firmness was more excellent than in the conventional comparative example.
[0047]
【The invention's effect】
As described in detail above, according to the present invention, it is possible to obtain a skin external preparation having an excellent effect in preventing and improving aging symptoms of the skin, a cell activator having an excellent effect, and an antioxidant having an excellent effect. Was completed.

Claims (8)

An external preparation for skin containing an extract of one or more plants of the genus Sesuvium . Sesubiumu genus (Sesuvium) skin external agent of claim 1 wherein the plant mils purslane (Sesuvium portulacastrum). A cell activator comprising, as an active ingredient, an extract of one or more plants of the genus Sesuvium . Sesubiumu genus (Sesuvium) cell activator according to claim 3, wherein the plant is mils purslane (Sesuvium portulacastrum). An external preparation for skin containing the cell activator according to claim 3 or 4. An antioxidant comprising, as an active ingredient, an extract of one or more plants of the genus Sesuvium . Antioxidants according to claim 6 Sesubiumu genus (Sesuvium) plant is a mill purslane (Sesuvium portulacastrum). An external preparation for skin containing the antioxidant according to claim 6.