JP2003176204A - Algicidal germicide - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To exterminate and prevent various algae and disease damages emerging during laver culture in low concentration in a short time by using a treatment solution which contains one or more kinds of an organic acid and an inorganic acid and iron sulfate and has <1.030 specific gravity of the solution. <P>SOLUTION: This algaecidal germicide contains one or more kinds of an organic acid and an inorganic acid and iron sulfate and has <1.030 specific gravity of the solution and exterminates and prevents various algae and disease damages emerging during laver culture. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a formulation for exterminating algae such as diatoms and aonori and pathogenic fungi such as red rot fungi, vase-shaped bacteria and adherent bacteria which are generated during seaweed cultivation.

[0002]

2. Description of the Related Art In seaweed cultivation, if algae such as diatoms propagate and adhere to the seaweed leaf body or the seaweed net, the growth of the seaweed may be hindered and eventually killed. Even if diatoms that have no problem in growth adhere to the leaves, if the original algae is used as a product, green dry spots will appear in the black dry seaweed and the commercial value will be reduced. Even if you eat it, it will be bitter and not delicious. In addition, red rot bacteria
If vase-shaped bacteria or adherent bacteria are generated, the seaweed may rot and become completely destroyed. Therefore, in order to control diseases such as red rot and weeds such as diatoms, work such as acid treatment and drying is performed. The work of draining has a drawback that it is hard work and poor in workability, but its effect is small. Although the present method of extermination by acid treatment is more effective than drought, it is not sufficient. There are also problems of increased costs and environmental load.

The following treatment agents for seaweed aquaculture have been disclosed so far. Japanese Unexamined Patent Publication (Kokai) No. 50-12125 discloses "Saturated aliphatic monocarboxylic acid having 1 to 4 carbon atoms, saturated or unsaturated dicarboxylic acid having 2 to 4 carbon atoms, glycolic acid, lactic acid, tartaric acid, malic acid, citric acid. And an algicidal agent containing one or more organic carboxylic acids selected from the group consisting of as an active ingredient. JP-A No. 11-286407 describes "a treatment agent for diatom control, which contains lactic acid and / or acetic acid and paraoxybenzoic acid ester". Japanese Examined Patent Publication No. Sho 60-13647 discloses "seaweed aquaculture by exterminating and preventing diseased algae, which is dipped in a treatment solution containing citric acid of 0.3 to 5.0% and pH of 1.0 to 6.0. Law "is described. Japanese Examined Patent Publication No. 60-13648 discloses "a seaweed aquaculture method for controlling weeds and diseases by adding inorganic acids such as hydrochloric acid, nitric acid, sulfuric acid and phosphoric acid to adjust the pH to 1.0 to 4.0". Have been described.

The acid treatment is carried out by removing the seaweed nets one by one and immersing the seaweed in the acid treatment solution for 10 to 15 minutes, and then resealing the seaweed net. Recently, because the number of farms per aquaculture has increased, the time required for treatment per one has to be shortened, and the acid treatment is called acid treatment, in which the ship is dipped while the seaweed net is fixed and the acid treatment is performed. Methods are being adopted. Since the acid treatment can be performed while the seaweed net is fixed, the acid treatment can be performed in 1/5 to 1/10 of the conventional time. However, since the treatment time is as short as 10 seconds to 5 minutes, the conventional 0.1 to 0.5 W / V% acid concentration has no effect and the treatment has been performed at a high concentration of 2 to 10 W / V%. . Still, it is not enough effect. The amount of use has increased dramatically, and the problems of cost increase and environmental pollution load are occurring.

A method has also been reported in which the effect of acid treatment is enhanced by adding an inorganic salt to increase the osmotic pressure (Japanese Patent Laid-Open No. 9-201180). However, in order to increase the specific gravity to 1.030 or more, the treatment liquid should contain several% to 10%.
Must be added. In order to obtain a constant treatment effect, the specific gravity of the treatment liquid must be kept constant,
It is a fairly difficult technique to put a large amount of salt in a continuous operation to keep its concentration constant, and the work efficiency is also poor. Generally, the treatment liquid is adjusted to about 1000 L and 40 to 100 laver nets are treated per day. Therefore, in order to maintain the salt concentration at 5%, the acidic treatment agent 2
0-40 L and 200-400 kg of salt are required. 40
It is difficult and very laborious to carry 0 kg of salt onto a small acid-treated ship and dissolve it to a certain specific gravity. Since seaweed cultivation is carried out in a highly closed inner bay, if all seaweed farmers perform acid treatment using this method, the specific gravity in seawater will increase and this may lead to an upset of the ecosystem. From the above, it is earnestly desired to develop a treatment agent and a treatment method which are highly safe and have a low concentration and excellent efficacy and workability.

[0006]

SUMMARY OF THE INVENTION The object of the present invention is to provide a low concentration of weeds such as diatoms and aonori and pathogenic bacteria such as red-rot fungi, vase-shaped bacteria and adherent bacteria without damaging the seaweed itself. -To provide a treatment agent and a treatment method that can be removed in a short time.

[0007]

[Means for Solving the Problems] As a result of extensive studies to solve the above problems, a treatment liquid containing at least one of organic acids and inorganic acids and iron sulfate and having a specific gravity of less than 1.030. By using the
It was found that disease control can be carried out at low concentration and in a short time. That is, the present invention is as follows. (1) Prevention of weeds and diseases that occur during seaweed cultivation, which is a solution containing one or more of organic acids and inorganic acids and iron sulfate, and has a solution specific gravity of less than 1.030 An algicidal fungicide that does. (2) Acid content is 0.01 to 2 W / V%, and iron sulfate content is 0.01 to 0.7 W / V%. Alga fungicide. (3) A treatment agent obtained by diluting a solution containing 1 to 99 W / V% acid and 1 to 99 W / V% iron sulfate.
The algicidal fungicide according to (1) or (2). (4) Iron sulfate is ferrous sulfate selected from the group consisting of ferrous sulfate, ferric sulfate, and basic ferric sulfate, (1) to (3) for seaweed cultivation Algicidal fungicide. (5) The treatment time is 10 seconds to 5 minutes, the algal disinfectant for seaweed cultivation according to (1) to (4).

As can be seen from the test examples, acid or iron sulfate alone has no effect of controlling red-rot fungi and diatoms, but by combining acid and iron sulfate, red-rot at a low acid concentration of 0.1-2%. It turned out that the bacteria can be exterminated. Moreover, 10
The red-rot fungus can be exterminated in a short time of from 5 seconds to 5 minutes.

The algicidal disinfectant of the present invention is obtained by diluting a treating agent consisting of a mixed solution of 1-99% acid and 1-99% iron sulfate with seawater. The treatment can be carried out with a solution containing acid and 0.01-0.7% iron sulfate. Alternatively, the acid and iron sulfate solutions may be separately diluted and used.

As the iron sulfate used in the present invention, one or more of ferrous sulfate, ferric sulfate and basic ferric sulfate can be used. The concentration of iron sulfate when using the algicidal agent of the present invention is preferably less than 1 W / V%. This is because if it is 1 W / V% or more, the seaweed is damaged and it cannot be used. When displayed with specific gravity,
It is less than 1.030. The specific gravity of seawater at the seaweed farm is
Since it is 1.015 to 1.023 and is optimally less than 1.030 when the specific gravity is 1.023, the preferable specific gravity range of the mixed treatment solution of the acid and iron sulfate of the present invention is 1.018.
It becomes ~ 1.028.

The acid used in the present invention may be one or more of organic and inorganic acids. Although there is no limitation on the type of acid, citric acid, malic acid, tartaric acid, lactic acid, fumaric acid, and amber are highly safe and recognized as food additives in view of safety and environmental load. Acid, acetic acid, gluconic acid, adipic acid, phytic acid,
It is preferable to use acids such as ketoglutaric acid, itaconic acid, phosphoric acid, hydrochloric acid, sulfuric acid and nitric acid. Acid concentration is 0.01
It is preferably ˜2 W / V%. 0.01 W / V%
This is because if it is lower than this, the effect is difficult to obtain, and if it exceeds 2 W / V%, damage to seaweed becomes stronger.

Amino acids, ammonium chloride, sodium nitrate, sodium nitrate, sodium nitrate, potassium nitrate, sodium phosphate, ammonium phosphate, potassium phosphate, ammonium nitrate, ammonium sulfate, and sugars can be added as fertilizer components.

(Test 1) Lactic acid 0.3 W / V% and ferric sulfate 0.01, 0.1, 0.2, 0.3, 0.5,
A solution dissolved in seawater was adjusted to 0.7 or 1.0 W / V%. The nori leaf cells infected with red rot fungus were treated at 10 ° C. for 30 seconds and 1 minute and then washed with sterilized seawater. Two days after the treatment, the control effect of the red rot fungus was examined under a microscope. As a comparative example, 0.3 W / V% lactic acid, 0.01 ferric sulfate,
0.1, 0.2, 0.3, 0.5, 0.7, 1.0W /
A V% solution was prepared. The evaluation criteria of the red rot control effect and the damage degree of seaweed are as follows. The specific gravity of the seawater used was 1.023. The results are shown in Tables 1 and 2. (Red rot control effect) −: No effect +: 20 to 50% control ++: 50 to 80% control +++: 80 to 100% control ++++: 100% control (damage to seaweed) −: No damage +: 20 -50% injury ++: 50-100% injury

[0014]

[Table 1]

[0015]

[Table 2]

(Test 2) Lactic acid 0.2 W / V% and ferric sulfate 0.1, 0.2, 0.5, 0.7, 1.0 W / V
The solution dissolved in seawater was adjusted so that it would be 100%. The nori leaf cells infected with red rot fungus were treated at 10 ° C. for 1 minute and 3 minutes and then washed with sterilized seawater. Two days after the treatment, the control effect of the red rot fungus was examined under a microscope. Lactic acid 0.2 as a comparative example
A W / V% solution was prepared. The specific gravity of the seawater used is
It was 1.023. The evaluation criteria for the effect of exterminating red rot bacteria and the damage degree of seaweed were the same as in Test 1. The results are shown in Table 3.
Shown in.

[0017]

[Table 3]

(Test 3) Lactic acid 0.1 W / V% and ferric sulfate 0.1, 0.2, 0.5, 0.7, 1.0 W / V
The solution dissolved in seawater was adjusted so that it would be 100%. The nori leaf cells infected with red rot fungus were treated at 10 ° C. for 3 minutes and 5 minutes and then washed with sterilized seawater. Two days after the treatment, the control effect of the red rot fungus was examined under a microscope. Lactic acid 0.1 as a comparative example
A W / V% solution was prepared. The evaluation criteria for the effect of exterminating red rot bacteria and the damage degree of seaweed were the same as in Test 1. The specific gravity of the seawater used was 1.023. The results are shown in Table 4.

[0019]

[Table 4]

(Test 4) Ferric sulfate 0.15 W / V% was added to lactic acid 0.01, 0.05, 0.2, 0.3, respectively.
The liquid dissolved in seawater was adjusted to 1.0 or 2.0 W / V%. Nori leaves infected with red rot fungus at 10 ℃
After treatment for 0 seconds, 30 seconds, and 3 minutes, it was washed with sterilized seawater. Two days after the treatment, the control effect of the red rot fungus was examined under a microscope. As a comparative example, lactic acid 0.01, 0.05, 0.2,
Solutions of 0.3, 1.0 and 2.0 W / V% were prepared. The evaluation criteria for the effect of exterminating red rot bacteria and the damage degree of seaweed were the same as in Test 1. Specific gravity of seawater used is 1.023
Met. The results are shown in Table 5.

[0021]

[Table 5]

(Test 5) 0.3 W / V% of lactic acid was added with 0.15 each of ferric sulfate, ferrous sulfate and basic ferric sulfate.
A liquid dissolved in seawater was prepared so that the W / V% was obtained. The nori leaf cells infected with red rot fungus were treated at 10 ° C. for 30 seconds and 1 minute and then washed with sterilized seawater. Two days after the treatment, the control effect of the red rot fungus was examined under a microscope. As a comparative example, ferric sulfate, ferrous sulfate, basic ferric sulfate 0.15 W / V
% Solution was prepared. The evaluation criteria for the effect of exterminating red rot bacteria and the damage degree of seaweed were the same as in Test 1. The specific gravity of the seawater used was 1.023. The results are shown in Table 6.

[0023]

[Table 6]

(Test 6) Ferric sulfate 0.15 W / V% with citric acid, malic acid, acetic acid, tartaric acid, phosphoric acid, respectively
A solution dissolved in seawater was prepared so that hydrochloric acid was 0.3 W / V%. Nori leaf cells infected with red rot fungus 30 at 10 ℃
After the treatment for 1 second and 1 minute, it was washed with sterilized seawater. Two days after the treatment, the control effect of the red rot fungus was examined under a microscope. As a comparative example, a 0.3 W / V% solution of citric acid / malic acid / acetic acid / tartaric acid / phosphoric acid / hydrochloric acid was prepared. The evaluation criteria for the effect of exterminating red rot bacteria and the damage degree of seaweed were the same as in Test 1. The specific gravity of the seawater used was 1.023. The results are shown in Table 7.

[0025]

[Table 7]

(Test 7) A liquid having the composition shown in Table 8 was prepared. Nori leaf bodies with diatoms (Likumophora) attached at 10 ° C
After treatment for 30 seconds and 1 minute, the product was washed with sterilized seawater.
Two days after the treatment, the diatom exterminating effect was examined under a microscope. The evaluation criteria for the diatom control effect and the damage degree of seaweed are as follows. The specific gravity of the seawater used was 1.023.
The results are shown in Table 8. (Diatom extermination effect)-: No effect +: 20-50% extermination ++: 50-80% extermination +++: 80-100% extermination ++++: 100% extermination (seaweed injury degree)-: No injury +: 20-50 % Injury ++: 50-100% injury

[0027]

[Table 8]

[0028]

EFFECTS OF THE INVENTION By using a treatment liquid containing one or more of organic acids / inorganic acids and iron sulfate and having a specific gravity of less than 1.030, the prevention of weeds and diseases caused by seaweed cultivation is prevented. It was found that it can be effectively and safely removed.

Claims (5)

[Claims] 1. A solution containing at least one of organic acids and inorganic acids and iron sulfate, and having a solution specific gravity of less than 1.030, which is a solution of weeds and diseases caused by seaweed cultivation. An algaecidal bactericidal agent for extermination prevention. 2. The acid content is 0.01 to 2 W / V%,
The content of iron sulfate is 0.01 to 0.7 W / V%, and the algaecide fungicide for seaweed cultivation according to claim 1. 3. 1 to 99 W / V% acid and 1 to 99 W / V
The algicidal fungicide according to claim 1 or 2, which is a treatment agent obtained by diluting a solution containing iron sulfate. 4. The seaweed aquaculture according to claim 1, wherein the iron sulfate is an iron sulfate selected from the group consisting of ferrous sulfate, ferric sulfate and basic ferric sulfate. Algicidal fungicide. 5. The algaecidal fungicide for seaweed cultivation according to claim 1, wherein the treatment time is 10 seconds to 5 minutes.