JP2976853B2 - Potato fungus inhibitor and method of using the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a medicament for selectively eliminating potato fungi parasitic in the cells of a laver leaf body and suppressing the occurrence and spread of potato fungi in the laver culture. .

[0002]

2. Description of the Related Art As a disease that causes a great deal of damage in laver culture, white blight, red scab, pot fungus and the like are generally well known. These diseases are caused by various germs attached to the laver leaves and fungi that are parasitic on the cells of the laver, and frequently occur and spread in a short time, and the degree of the spread is severe. Therefore, countermeasures against these diseases are extremely important issues in laver culture.

[0003] As a method of controlling various fungi attached to the laver leaf and the laver net, a method of drying the laver net and a method of freezing and storing (conventional example) have been known for a long time. However, these methods do not kill the pot bacilli sufficiently.

Further, Japanese Patent Application Laid-Open No. Sho 50-112425 (conventional example) discloses saturated aliphatic monocarboxylic acids having 1 to 4 carbon atoms, saturated or unsaturated dicarboxylic acids having 2 to 4 carbon atoms, and glycolic acid. , Lactic acid, tartaric acid, malic acid,
There is disclosed a drug having one or more organic carboxylic acids selected from the group consisting of citric acid as an active ingredient, and a method of immersing a glue net or the like in this drug is disclosed. The conventional example effectively acts as a low-toxicity agent for controlling algae.

[0005] However, since the pot-like fungi are small cells and parasite in the glue cells, even if the organic carboxylic acid as an active ingredient is subjected to a immersion treatment at a very high concentration, the control effect on the pot-like fungi is still small. In addition, because the concentration becomes higher and the pH of the solution becomes lower, it causes the problem that glue cells are killed.In the conventional example, the fungus is selectively removed without damaging the glue cells. It is extremely difficult to do.

In addition, as disclosed in JP-A-59-159725, a method of using a hydrogen chloride preparation in which hydrogen chloride is mixed with seawater and a malachite green preparation at the same time (conventional example); As shown in JP-A-313403, a method (conventional example) of spraying or applying a glue disease controlling agent obtained by dissolving chitosan in seawater to a glue net has been proposed.

However, in the conventional example, there is a problem that handling of hydrogen chloride is dangerous, and malachide green is toxic, so that the image of glue as a health food is deteriorated. In addition, the conventional example is not practical because chitosan has extremely low solubility in seawater.

In addition, JP-A-7-17806 discloses a pesticide containing monosodium fumarate and / or monopotassium fumarate and an organic carboxylic acid (conventional example). It is effective in suppressing

[0009] However, the scab is a disease caused by the scab of the genus Pythium, which is characterized in that the hypha penetrates the glue cells and takes up the cell contents as nutrients, whereas the scab mold is The disease is Lagenidial
It is a disease caused by a kind of fungus of the genus Olpidiopsis of the genus Esca, which does not have a temporary root for nutrient absorption, and is parasitic on the cells of the lamina thallus. It grows, and these stinging disease and pot fungus are completely different diseases. Therefore, it was not possible to predict whether the conventional example would be effective against pot fungus disease.

[0010]

As described above, various proposals have been made for the control of glutinous disease, but none of them has been particularly effective against the fungus disease. In the present invention, in the Nori cultivation and fishing ground where the infestation of the fungal disease has been confirmed, or in the Nori cultivation and fishing ground where the fungal disease has further spread, there is no adverse effect on the laver leaf, and the fungal disease is suppressed in a short time. It is an object of the present invention to provide a potable fungus inhibitor which can be used.

[0011]

The agent for inhibiting fungal disease according to the present invention contains monosodium fumarate and / or monopotassium fumarate, and is 0.1 to 10 times the weight thereof. The gist of the invention is that it contains fumaric acid. Alternatively, the bacillus disease inhibitor of the present invention contains monosodium fumarate and / or monopotassium fumarate, and further contains a water-soluble phosphorus compound in an amount of 0.1 to 10 times (weight ratio) thereof. The gist is that Furthermore, the method of using the pot fungus inhibitor according to the present invention is characterized in that the pot fungus inhibitor is dissolved in water or seawater and used at a pH of 2.3 to 3.0.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, the combination of the above-mentioned chemicals enables the production of glutinous leaf cells even if the pH and the concentration are not so high as to adversely affect the glutinous leaves in glutinous culture. Potassium fungi that are parasitic on.

The active ingredients of the present invention, monosodium fumarate, monopotassium fumarate, fumaric acid (organic carboxylic acid) and phosphorus compounds, can be easily dissolved in water or seawater. And can be dissolved in seawater, so it is convenient to use in the field of Nori culture.

The pot-like fungus inhibitor of the present invention is provided as a powder, a concentrated solution, or a dilute solution. It is recommended that the agent be dissolved in water or seawater before use. P of the lysate
H is preferably from 1.5 to 3.5, more preferably pH
In the range of 2.0 to 3.0, even more preferably pH
2.3 to 3.0. If the pH is lower than 1.5, there is a risk that the glutinous leaf itself will die, while if the pH is higher than 3.5, the control effect is reduced.

The content of the monosodium fumarate and / or the monopotassium fumarate in the present invention is as follows:
It is preferably 0.01 to 3.0% by weight, more preferably 0.03 to 2.0% by weight. If the amount is less than 0.01% by weight, the inhibitory effect on the fungal disease is insufficient, while if the concentration exceeds 3.0% by weight, the inhibitory effect on the fungal disease is saturated. Among these, fumaric acid monosodium salt is most preferable because it is inexpensive.

As described above, the potato fungus inhibitor of the present invention is most effective in the pH range of 2.3 to 3.0.
Therefore, for example, the pH when fumaric acid monosodium salt is dissolved in seawater is 3.5, and the pH is lowered using a water-soluble organic carboxylic acid and / or a water-soluble phosphorus compound.

In the case where disodium fumarate or dipotassium fumarate is used instead of monosodium fumarate or monopotassium fumarate, a water-soluble organic carboxylic acid or a water-soluble organic carboxylic acid must be used to achieve the above pH range. It is not appropriate because the added amount of the sex phosphorus compound becomes too large and adversely affects the Nori leaf.

The organic carboxylic acid in the present invention includes
Among the recognized food additives, compounds that rapidly dissolve and decompose in seawater are recommended. Specifically, lactic acid, tartaric acid, malic acid, citric acid, fumaric acid, succinic acid, gluconic acid and the like are recommended. No. One of these organic carboxylic acids may be used, or a mixture of two or more thereof may be used.

In order to adjust the pH so as to effectively exert the effect of the fungus inhibitor of the present invention, the mixing ratio of the organic carboxylic acid to the monosodium fumarate and / or the monopotassium fumarate is preferably fumaric acid. The organic carboxylic acid is preferably 0.1 to 10 times, more preferably 0.2 to 5.0 times, the unit weight of the monosodium salt and / or monopotassium fumarate.

Examples of the phosphorus compound in the present invention include inosit hexaphosphoric acid, orthophosphoric acid, phosphoric anhydride, phosphorous acid, hypophosphorous acid, and phosphates such as sodium phosphate and ammonium phosphate. These are converted to phosphoric acid when dissolved in seawater, or hydrolyze to phosphoric acid in the presence of an acid, and act to lower the pH. One of these phosphorus compounds may be used, or two or more of them may be used in combination. Phosphorus acts as a nutrient for the growth of glutinous leaves and it is said that the luster of the glue is improved. These advantages also make phosphorus compounds suitable as acids for lowering the pH.

Similarly to the above, in order to adjust the pH so as to effectively exert the effect of the present bacterium fungus inhibitor, the mixing ratio of the phosphorus compound is set to the monosodium fumarate and / or the monopotassium fumarate. 0 .0 per unit weight.
It is preferably 1 to 10 times, more preferably 0.2 to 5.0.
It is twice.

When the potato bacterium inhibitor of the present invention is used by dissolving it in water or seawater and immersing the object in the solution, the immersion time is preferably 1 to 30 minutes. , More preferably in the range of 2 to 15 minutes. If the immersion time is less than 1 minute, the extermination effect is insufficient, and 3
This is because if the immersion is carried out for a long time exceeding 0 minute, the glutinous leaf somatic cells may be killed.

The agent for inhibiting fungal disease according to the present invention can also be used by spraying it onto an object to be treated. In the case of spraying, it is desirable that the inhibitor be washed away with seawater or water within 10 minutes since the leaf cells may be killed if left for a long time. In addition, various additives such as sodium nitrate, sodium hydrogen phosphate, vitamin B ₁₂ , and a chelating agent may be used for the growth of glue and quality improvement.

[0024]

EXAMPLES The present invention will be specifically described below with reference to examples and comparative examples, but the present invention is not limited to these examples.

<Examples 1 to 6 and Reference Example 1> Each of the components of the bacterium fungus inhibitor was dissolved in seawater so as to have the concentrations shown in Table 1 to prepare inhibitor solutions. P of the inhibitor solution
H was as shown in Table 1, and the liquid temperature was 15 ° C. In addition, W-300 used in Example 6 is a commercially available activator (Nori acid treating agent manufactured by Fuso Chemical Industry Co., Ltd.) and contains 4.6% of total phosphorus.

Nori leaf bodies infected with 3 to 5% of the pot mold disease were immersed in the above inhibitor solution for 7 minutes. In addition, the pot-like fungus-infected Nori leaf was prepared by infecting healthy nori leaf with 3-5% of the frozen diseased leaf obtained from the Ariake Fisheries Experimental Station in Fukuoka Prefecture in November 1993.

After the immersion treatment, the laver leaves are washed with sterilized seawater.
And the seaweed PES with nutrients and seaweed leaf (component: NaN
O_Three , Na_Two-glycerophosphate, Fe-EDTA, Vita
Min B ₁₂, Cl, etc.) 200ml to 300ml beaker
And cultured. Culture conditions are 5000Lux
The light period was 10 hours a day, and aeration culture was performed at 15 ° C. Through
After 1 day, 3 days, and 5 days after air culture,
Microscopic observation of several bodies (150x and 600x)
And the ratio of the lesion to the total area.
It was a constant index. Vases grow even after 5 days of culture
If not, it was determined that there was an inhibitory effect.

In the same manner as described above, the glutinous leaf body to which various fungi are adhered was added to the solution of the fungus inhibitor of the present invention for 7 days.
After immersion for a minute, aeration culture was performed, the number of various bacteria adhering to the Nori leaf was observed, and the control rate was determined. At the same time, dead cells of the Nori leaf were also observed, and the Nori leaf dead cell rate was determined. Table 1 shows the results of Examples 1 to 6 and Reference Example 1.

[0029]

[Table 1]

<Comparative Examples 1 to 4> Each component was dissolved in seawater so as to have the concentration shown in Table 2 to prepare a solution of an inhibitor. The pH of the inhibitor solution of each comparative example is as shown in Table 2. The same treatment as in the above example was performed using these inhibitor solutions. Table 2 shows the results.

[0031]

[Table 2]

As can be seen from the above results, in the case of Comparative Examples 1 to 4 in which monosodium fumarate, citric acid, phosphoric acid, or a commercially available activator was used alone, for example, the percentage of vasculature lesions after 5 days Examples 1 to 6 in which monosodium fumarate and / or monopotassium fumarate were used in combination with fumaric acid or a phosphorus compound, although the potency of potato disease was not observed. Showed no adverse effects on the laver leaves, a high inhibitory effect on pot fungus disease, and also suppressed subsequent growth.

In Examples 1 to 6, an effect of controlling various fungi was also confirmed. The germs are bacteria attached to the laver leaves and are not the causative agents of blight, scab, and potato disease. Poor salt absorption. The agent for inhibiting fungal disease of the present invention effectively acts on various fungi, and if the bacteria are clean without adhering to the cell wall surface of the Nori leaf, nutrient absorption is improved and growth is promoted.

<Example 7> In November 1993, at a pole-type laver culture fishery in the Ariake Sea area of Fukuoka Prefecture, 3% of the pot-like fungi parasitized on the laver leaves growing to 8 to 10 cm by expanding the laver net. The test was performed to control potato nets infected with this potato disease.

As a solution of the potato disease inhibitor, a solution prepared by dissolving fumaric acid monosodium salt in seawater to give 0.20% by weight of fumaric acid, 0.10% by weight of fumaric acid, and 0.15% by weight of citric acid is used. (The concentration of the potase disease inhibitor was 0.45% by weight).
The pH of the inhibitor solution was 2.7.

Four nets to be treated were treated at a seawater temperature of 14 ° C. for 5 to 8
Soak for minutes. Nori cultivation was continued after that, and after 3 days and 5
A day later, the inhibitory effect on the fungal disease was observed. As a result, the fungus disease was suppressed, no adhesion of various fungi was observed, and no subsequent growth was observed. The state of the vegetative cells of the glue leaves was extremely healthy, the growth was fast, and soft, high quality glue was obtained.

[0037]

EFFECTS OF THE INVENTION By using the agent for inhibiting fungal disease according to the present invention, the fungus parasitic on the nori leaf cells can be eliminated without adversely affecting the nori leaf body, thereby suppressing the fungal disease. be able to. In addition, even when glue leaves are infested with pot fungus, they can be suppressed in a short time by dipping or spraying with a low concentration pot fungus inhibitor. Safe for you.

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Claims (3)

(57) [Claims] [Claim 1] contains fumaric acid mono sodium salt and / or fumaric acid monopotassium salt, the 0.1
An agent for inhibiting fungal disease, comprising 0 times (weight ratio) fumaric acid . (2) Monosodium fumarate and / or
Contains monopotassium fumarate, and further contains 0.1-1
0 times (weight ratio) of water-soluble phosphorus compound
A fungus disease suppressant characterized by the following. (3) The bacterium fungus control according to claim 1 or 2.
Dissolve the agent in water or seawater and use at pH 2.3-3.0
A method for using a bacterium fungus inhibitor, which comprises: