CN115886035A

CN115886035A - Algae inhibitor and preparation method thereof

Info

Publication number: CN115886035A
Application number: CN202211366541.7A
Authority: CN
Inventors: 林猷宪; 张士轩; 刘家兴; 林骏睿; 林定纬
Original assignee: Inner Mongolia Nonghokang Agricultural Biotechnology Co ltd
Current assignee: Inner Mongolia Nonghokang Agricultural Biotechnology Co ltd
Priority date: 2022-11-01
Filing date: 2022-11-01
Publication date: 2023-04-04

Abstract

The invention belongs to the technical field of algae inhibition, and particularly relates to an algae inhibitor and a preparation method thereof. The algae inhibitor comprises: benzoic acid, edible oil, detergent, trehalose and phosphorus-dissolving bacteria. The benzoic acid, the edible oil, the detergent, the trehalose and the phosphorus-dissolving bacteria are pollution-free materials, and the pollution-free materials are utilized to increase the reproduction of soil microbial flora so as to degrade spirogyra generating factors and effectively kill spirogyra in rice fields. Through field test comparison for 3 years, the effect of the algae inhibitor is far more remarkable than that of copper sulfate, the death rate of the water cotton reaches 99%, and the dead water cotton residues are reduced and decomposed into organic fertilizers which can be absorbed and utilized by soil.

Description

Algae inhibitor and preparation method thereof

Technical Field

The invention belongs to the technical field of algae inhibition, and particularly relates to an algae inhibitor and a preparation method thereof.

Background

Rice is a food crop specially needed in China, and paddy fields are planted in many countries. In the planting process, because the paddy field uses excessive diammonium phosphate or phosphate fertilizer to generate long phosphorization or organic fertilizer is not applied to generate water cotton without decomposition, the water cotton can grow rapidly along with the rise of the temperature after transplanting, and in severe cases, the water cotton grows to cover seedlings, so that the seedlings die, and farmers want to cry and lacrimation.

At present, copper sulfate is generally used for killing spirogyra, although the method has high speed and obvious effect of killing spirogyra, the method has the defects that secondary pollution is generated on a water body, and organisms such as fish and the like are possibly poisoned, so that toxic substances are enriched, and the health of human is harmed. In addition, the harm of soil environmental pollution and heavy metal exceeding standard caused by copper sulfate is far larger than the harm increased by spirogyra.

Disclosure of Invention

Based on the technical problems, the invention provides an algae inhibitor and a preparation method thereof, which utilize pollution-free materials to increase the reproduction of soil microbial flora so as to degrade spirogyra production factors and effectively kill spirogyra in rice fields.

The specific technical scheme is as follows:

the invention discloses an algae inhibitor, which is used for killing spirogyra in a paddy field, and comprises the following components:

benzoic acid with the concentration of 0.3-0.7 g/L;

edible oil with the concentration of 50-75 percent;

20-40% of cleaning agent;

trehalose with the concentration of 3-7%;

the concentration is 0.2-0.4 g/L, 1-3 hundred million/g viable count phosphorus-dissolving bacteria.

In the above technical solution, the algae inhibitor specifically includes:

benzoic acid with a concentration of 0.5 g/L;

edible oil with concentration of 65%;

30% of cleaning agent;

trehalose at a concentration of 5%;

the concentration is 0.3g/L, 2 hundred million/g viable count phosphorus-dissolving bacteria.

In the technical scheme, the edible oil is at least one of sunflower oil, soybean oil and palm oil.

In the technical scheme, the cleaning agent is a detergent.

The invention also discloses a preparation method of the algae inhibitor, which comprises the following steps:

diluting phosphorus-dissolving bacteria with water, and uniformly spraying the phosphorus-dissolving bacteria to a paddy field;

sequentially pouring edible oil, detergent and trehalose into a container, and uniformly stirring to prepare an emulsifier;

mixing benzoic acid with emulsifier, and spraying on paddy field.

In the above technical scheme, add water to dilute the phosphorus-solubilizing bacteria, evenly spray in paddy field, specifically include:

300 g/mu of land phosphorus-dissolving bacteria are diluted by 100 times and evenly sprayed on the paddy field.

In the technical scheme, the edible oil, the detergent and the trehalose are sequentially poured into a container and uniformly stirred to prepare the emulsifier, which specifically comprises the following steps:

firstly, 65% of sunflower oil is poured into a 1000 ml container, then 30% of liquid detergent and 5% of trehalose are sequentially poured into the 1000 ml container, and the mixture is stirred for 5-8 minutes at room temperature to prepare the emulsifier.

In the above technical scheme, mixing benzoic acid and an emulsifier, evenly spraying in paddy field specifically includes:

after 500 g/mu of benzoic acid and the emulsifier are diluted by 300 times, the mixture is evenly sprayed on the paddy field.

Compared with the prior art, the algae inhibitor and the preparation method thereof have the beneficial effects that:

the invention adopts benzoic acid, edible oil, detergent, trehalose and phosphorus-dissolving bacteria which are all pollution-free materials, and the pollution-free materials are utilized to increase the reproduction of soil microbial flora so as to degrade spirogyra generating factors and effectively kill spirogyra in the paddy field. Compared with field tests for 3 years, the algae inhibitor has a remarkable effect compared with copper sulfate, the death rate of the water cotton reaches 99%, and the dead water cotton residues are reduced and decomposed into organic fertilizers which can be absorbed and utilized by soil.

Drawings

FIG. 1 is a flow chart of a method for preparing an algal inhibitor according to the present invention;

FIG. 2 is a schematic view of the growth of cotton in the control area of the present invention;

FIG. 3 is a schematic view of the growth of cotton in the control area according to the present invention;

FIG. 4 is a schematic diagram showing the comparison of the growth of cotton wool in the control area and the control area according to the present invention;

FIG. 5 is a schematic view of the present invention controlling the growth of cotton wool;

FIG. 6 is a schematic view of the growth of the copper sulfate group cotton of the present invention;

FIG. 7 is a schematic diagram showing the growth of cotton wool with a treatment group concentration of 0.5g/L according to the present invention;

FIG. 8 is a schematic diagram showing the growth of cotton wool with a concentration of 0.1g/L in the treatment group according to the present invention;

FIG. 9 is a schematic representation of the growth of cotton wool at a concentration of 0.05g/L in the treatment group of the present invention.

Detailed Description

The invention will be further described with reference to specific embodiments and drawings, but the invention is not limited to these embodiments.

Example 1

In the present example, the parameters relating to the percentages are in mass percent.

benzoic acid with the concentration of 0.3-0.7 g/L; edible oil with the concentration of 50-75 percent; 20-40% of cleaning agent; trehalose with the concentration of 3-7%; the concentration is 0.2-0.4 g/L, 1-3 hundred million/g viable count phosphorus-dissolving bacteria.

As a most preferred embodiment, the algae inhibitor specifically comprises: benzoic acid with a concentration of 0.5 g/L; edible oil with concentration of 65%; 30% of cleaning agent; trehalose at a concentration of 5%; the concentration of the phosphate solubilizing bacteria is 0.3g/L and the viable count is 2 hundred million/g.

As a further preferable embodiment, the edible oil is preferably at least one of sunflower oil, soybean oil, and palm oil. The cleaning agent is preferably a liquid detergent.

The invention adopts benzoic acid, edible oil, detergent, trehalose and phosphorus-dissolving bacteria which are all pollution-free materials, the phosphorus-dissolving bacteria can effectively decompose and control phosphorus fertilizers in soil to generate water cotton factors, the benzoic acid is a food-grade preservative and can kill algae, the sunflower oil, the detergent and the trehalose are prepared into an emulsifier, the emulsifier is a natural surfactant and can prevent oxygen on the water surface from causing water cotton to be incapable of breathing, in addition, the pollution-free materials are utilized to increase the reproduction of soil microbial flora so as to degrade water cotton generation factors, and the reproduction and growth of water cotton spores can be effectively killed. Compared with field tests of 3 years, the formula has the advantages that the effect is more remarkable than that of copper sulfate, the mortality rate of the water cotton reaches 99%, most importantly, the incidence rate of the water cotton is reduced by more than 50% every other year, the survival rate of seedlings is improved, and the division rate of 27377is increased. In addition, the dead water cotton residues are reduced and decomposed into organic fertilizers which can be absorbed and utilized by soil, the fertilizer consumption of the same acre is reduced by 10-15%, and the utilization rates of the phosphate fertilizers and the organic fertilizers are further improved, so that the yield is increased.

Example 2

As shown in fig. 1, the invention also discloses a preparation method of the algae inhibitor, which comprises the following steps:

step S1: diluting phosphorus-dissolving bacteria with water, and uniformly spraying the phosphorus-dissolving bacteria to the paddy field.

Step S2: sequentially pouring edible oil, detergent and trehalose into a container, and stirring to obtain the emulsifier.

And step S3: mixing benzoic acid with emulsifier, and spraying on paddy field.

In this embodiment, can utilize the stirring oscillator to stir, realize even stirring.

The invention uniformly sprays the pesticide to the paddy field by adopting a secondary spraying mode because the benzoic acid has a sterilization effect, and the phosphorus-dissolving bacteria and the benzoic acid have conflict at high concentration, thereby reducing the effect of killing the cotton wool.

Example 3

In the embodiment, taking phosphorus-solubilizing bacteria, sunflower oil, liquid detergent, trehalose and benzoic acid as examples, the specific steps are as follows:

firstly, 300 g/mu of phosphate solubilizing bacteria are diluted by 100 times and evenly sprayed to the paddy field.

Secondly, firstly pouring 65% of sunflower oil into a 1000 ml container, then pouring 30% of liquid detergent and 5% of trehalose into the 1000 ml container, and stirring for 5-8 minutes at room temperature to prepare the emulsifier uniformly.

Finally, after diluting benzoic acid of 500 g/mu with emulsifier 300 times, evenly spraying the mixture on the paddy field.

By adopting the technical scheme in the embodiment 3, the mortality rate of the water cotton reaches 99 percent, and most importantly, the incidence rate of the water cotton is reduced by more than 50 percent every other year, the survival rate of the seedlings is improved, and the division 27377 rate is increased. In addition, the dead water cotton residues are reduced and decomposed into organic fertilizers which can be absorbed and utilized by soil, the fertilizer consumption of the same acre is reduced by 10-15%, and the utilization rates of the phosphate fertilizers and the organic fertilizers are further improved, so that the yield is increased.

Example 4

The cotton wool-removing and killing test in paddy fields was carried out in saline and alkaline fields at the red light farm of Daan city, daan, jilin province, and Jilin province 6 months in 2022. The area is divided into a control area test and a control area test, and the area is 1 hectare.

1. The control area adopts solvents comprising: bacillus safensis (Bacillus safensis) 3000 g/hectare; after 1 hour, preparing 65 percent of sunflower oil, 30 percent of liquid detergent and 5 percent of trehalose into 3000 ml of emulsifier per hectare; benzoic acid 5000 g/ha. The mixed spray was carried out as in example 2.

2. The control area is prepared by adopting solvents comprising: copper sulfate 5000 g/hectare.

In order to overcome the influence caused by uneven spraying, the unmanned aerial vehicle is sprayed by the same unmanned aerial vehicle.

FIG. 2 (a) is a schematic view showing an initial stage of generation of cotton wool in the control area; (b) The figure is a schematic view of the growth of the cotton on the first day after the pesticide is applied to the control area; (c) The figure is a schematic view of the growth of the absorbent cotton in the third day after the pesticide is applied in the control area; (d) The figure is a schematic view of the growth of the cotton wool in the seventh day after the pesticide is applied in the control area; (e) The figure is a schematic view of the growth of the cotton wool in the tenth day after the pesticide is applied to the control area; (f) The figure is a schematic view of the growth of the cotton wool in the twelfth day after the pesticide is applied in the control area; (g) The figure is a schematic diagram of the growth of cotton wool twenty days after the control area is applied.

As can be seen from the graph (b) in fig. 2, no bubble was generated on the first day after the control region was administered, and as can be seen from the graph (d) in fig. 2, the water quality was clear on the seventh day after the control region was administered, whereas with the anhydrous cotton, as can be seen from the graph (e) in fig. 2, the growth was good on the tenth day after the control region was administered, and as can be seen from the graph (f) in fig. 2, the growth was vigorous on the twelfth day after the control region was administered. As can be seen from the graph (g) in FIG. 2, the water quality is clear twenty days after the application of the pesticide to the control area, the rice grows vigorously, and no weeds are generated in the peanuts.

FIG. 3 (a) is a schematic view showing an initial generation of cotton wool in the control area; (b) The figure is a schematic diagram of the growth of the cotton wool on the first day after the drug is applied to the control area; (c) The figure is a schematic view of the growth of the cotton wool on the third day after the pesticide is applied to the control area; (d) The figure is a schematic view of the growth of the cotton wool on the fifth day after the drug is applied to the control area; (e) The figure is a schematic view of the growth of the cotton wool on the tenth day after the drug is applied to the control area; (f) The figure is a schematic view of the growth of cotton wool on the twelfth day after the control area was applied.

As can be seen from the graphs (b) and (c) in FIG. 3, bubbles were generated three days before the administration of the control region, from the graph (d) in FIG. 3, no bubbles were generated on the fifth day after the administration of the control region, from the graph (e) in FIG. 3, from the tenth day after the administration of the control region, from the graph (f) in FIG. 3, and from the twelfth day after the administration of the control region, from the anhydrous cotton.

FIG. 4 is a schematic diagram showing the comparison between the growth of rice and that of rice in the control area and the control area after removing cotton, and it can be seen from FIG. 4 that the investigation result 12 days after the control area is shown on the left side shows that the effect of removing cotton is good, but the color of rice turns yellow, and the number of rice is min 27377. The investigation result 12 days after the control area is shown on the right, the color of the rice is dark green, and more rice grows vigorously and has no weed at 27377.

In red-light farms in Songyuan city of Jilin province, rice production cooperation society in Daan city has field tests for more than 3 years, the effect is remarkable, the effect of removing the water cotton reaches about 99%, and the effect is better than that of copper sulfate (the copper sulfate takes effect in the second day, the formula takes effect in two days, but the death degree of the water cotton is higher than that of the copper sulfate), and the early growth and green return of the rice are promoted, namely, 27377.

Example 5

The invention adopts algae inhibitors with different concentrations for experiments, the algae inhibitors with different concentrations are divided into treatment groups, the control group does not use any medicine at all, the copper sulfate group adopts 1g of copper sulfate for 100 times dilution, and the specific realization result is shown in table 1.

TABLE 1 experimental comparison scheme

From table 1, the following conclusions can be drawn:

1. control group served as control group: the weight of the cotton wool before the test is 5g, the weight of the cotton wool after the test is 5.17g after the test is 3 days, and the weight is 0.17g, which indicates that the cotton wool continues to propagate and grow and is greenish, and the specific structure is shown in (a) diagram and (b) diagram in fig. 5, wherein, (a) is a schematic diagram of the growth appearance of the control cotton wool, and (b) is a schematic diagram of the growth of the control cotton wool.

2. Copper sulfate group: similarly, 200ml of water was added after 24-hour incubation, the weight before the test was 5g, the weight after 3 days was 4.73g, and the weight was reduced by 0.27g, which indicates that the cotton wool inhibited growth and had a withered and yellow color, as shown in (a) and (b) of FIG. 6, wherein (a) is an appearance diagram of the cotton wool of copper sulfate group and (b) is a partially enlarged diagram of the growth of the cotton wool of copper sulfate group.

3. Treatment group concentration 0.5g/L: similarly, 200ml of water was added after 24 hours of culture, the weight before the test was 5g, the weight after 3 days was 3.89g, and the weight was reduced by 1.11g, which indicates that the cotton wool completely inhibited the growth of propagation and died, as shown in (a) and (b) of FIG. 7, wherein (a) is an appearance diagram of the cotton wool growth at a concentration of 0.5g/L in the treated group, and (b) is a partial enlarged diagram of the cotton wool growth at a concentration of 0.5g/L in the treated group.

4. Treatment group concentration 0.1g/L: similarly, 200ml of water was further added after 24 hours of culture, the weight before the test was 5g, the weight after 3 days was 3.67 g, and the weight loss was 1.33 g, which indicates that the cotton wool completely inhibited withering, as shown in (a) and (b) of FIG. 8, wherein (a) is an appearance diagram of the growth of the cotton wool at a concentration of 0.1g/L in the treated group, and (b) is a partial enlarged diagram of the growth of the cotton wool at a concentration of 0.1g/L in the treated group.

5. Treatment group concentration 0.05g/L: similarly, 200ml of water was used for culturing for 24 hours, and then the weight of the composition before the test was 5g, the weight of the composition after 3 days was 4.61g, and the weight of the composition was 0.39g, which indicates that the cotton wool had inhibited the growth of propagation and died, as shown in the (a) diagram and the (b) diagram in FIG. 9, wherein (a) is an appearance diagram of the growth of the cotton wool with the treatment group concentration of 0.05g/L, and (b) is a partial enlarged diagram of the growth of the cotton wool with the treatment group concentration of 0.05 g/L.

The experiments prove that the cotton wool inhibitor has better inhibiting and killing effects than copper sulfate on cotton wool in different concentrations.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification made within the spirit and principle of the present invention should be included within the scope of protection of the present invention.

Claims

1. An algal inhibitor for killing spirogyra in paddy field, comprising:

benzoic acid with the concentration of 0.3-0.7 g/L;

edible oil with the concentration of 50-75 percent;

20-40% of cleaning agent;

trehalose with the concentration of 3-7%;

2. The algal inhibitor of claim 1, wherein the algal inhibitor comprises:

benzoic acid with a concentration of 0.5 g/L;

65% edible oil;

30% of cleaning agent;

trehalose at a concentration of 5%;

the concentration of the phosphate solubilizing bacteria is 0.3g/L and the viable count is 2 hundred million/g.

3. The algal inhibitor of claim 1 or 2 wherein the edible oil is at least one of sunflower oil, soybean oil, and palm oil.

4. The inhibitor according to claim 1 or 2, wherein the detergent is a detergent.

5. The method of claim 1, wherein the method comprises:

sequentially pouring the edible oil, the detergent and the trehalose into a container, and uniformly stirring to prepare an emulsifier;

mixing benzoic acid with emulsifier, and spraying on paddy field.

6. The method for preparing the algae inhibitor according to claim 5, wherein the method for diluting the phosphorus-solubilizing bacteria with water and uniformly spraying the diluted phosphorus-solubilizing bacteria on the paddy field comprises the following steps:

7. The method for preparing algae inhibitor according to claim 5, wherein the step of pouring the edible oil, the detergent and the trehalose into a container, and stirring them uniformly to prepare the emulsifier comprises:

8. The method for preparing the algae inhibitor according to claim 5, wherein the mixing of benzoic acid and the emulsifier and the uniform spraying of the mixture on the paddy field comprises the following steps:

after 500 g/mu of field benzoic acid and an emulsifier are diluted by 300 times, the mixture is uniformly sprayed on a paddy field.