JP2003095961A - Skin beautifying promoter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin beautifying promoter capable of raising water holding properties of the skin and affording the moist skin with a fine texture by oral ingestion. SOLUTION: This skin beautifying promoter is obtained by including an enzymic hydrolyzate of a swallow's nest as an active ingredient. The enzymic hydrolyzate is preferably a hydrolyzate with a protease. The molecular weight of the enzymic hydrolyzate is preferably 2,000-70,000. The skin beautifying promoter preferably contains collagen and/or its enzymic hydrolyzate and further preferably contains at least one kind selected from glucosamine, a nucleic acid, an SOD like substance, an SOD activity inducer and lactoferrin. Furthermore, at least one kind of a cultured product obtained by culturing lactic acid bacteria, enterococci, yeasts or fungi is preferably contained.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a skin beautifying agent capable of improving moisturization of the skin and obtaining finely textured skin by ingestion.

[0002]

2. Description of the Related Art Skin troubles such as rough skin and fine wrinkles are serious problems especially for women. In skin tissue,
As a component that is greatly involved in its water retention and elasticity,
Known are acidic mucopolysaccharides such as hyaluronic acid and chondroitin sulfate, and collagen.

The above-mentioned acidic mucopolysaccharide has a high water retention property,
It binds to collagen, which functions as a pillar of the intercellular matrix, and is distributed in a large amount in connective tissue, cartilage tissue, skin tissue, etc., and serves to maintain the function and morphology of cells.

When the amount of these decreases due to aging or the like, the water retention and elasticity of the skin are lost, causing rough skin and fine wrinkles.

Therefore, in order to prevent and improve skin roughness and fine wrinkles, it is important to maintain the moisture and tension of the skin.

[0006] Conventionally, cosmetics and beauty health foods containing various components having a skin moisturizing property and elasticity maintaining effect are commercially available. Examples of such components include hyaluronic acid, mucopolysaccharides such as chondroitin sulfate described above, proteins such as collagen, trehalose, low-molecular-weight sugars such as sorbitol, vitamins, amino acid derivatives, and the like.
Examples thereof include ceramide, α-oryzanol, oils and fats such as refined camellia oil, and more recently, naturally derived ingredients having high safety tend to be respected.

Specifically, a beauty and health food characterized by containing mucopolysaccharide containing hyaluronic acid and chondroitin sulfate, collagen and nucleic acid (Japanese Patent Laid-Open No. 10-165).
No. 138), an active oxygen scavenging factor, an anti-allergic factor, a skin-improving factor, and an antioxidative factor.
-70), a food comprising conchiolin or a processed product thereof (JP-A-8-173091), a health food having a complex mucopolysaccharide in which a mucopolysaccharide and a peptide are bound (JP-A-9-98739), and a ceramide. Health food (JP-A-11-113530) and the like can be mentioned.

[0008]

However, although the cosmetics containing the above-mentioned components can increase the water retention on the skin surface, the effect is only temporary, and the effect on the skin is fundamentally increased. It did not improve the condition.

Further, even if the above beauty and health food is ingested, the effect is not always satisfactory.

Therefore, an object of the present invention is to provide a skin beautifying agent which, when orally ingested, enhances the water retention of the skin and makes it possible to obtain a moist and fine skin.

[0011]

Means for Solving the Problems As a result of intensive studies to achieve the above-mentioned object, the present inventors have found that the ingestion of the enzymatic decomposition product of the swallow pit gives the skin a moist feeling and less desquamation. Based on this finding, the present invention has been completed.

That is, the skin beautifying agent of the present invention is characterized by containing an enzyme decomposition product of a swallow as an active ingredient.

In the present invention, it is preferable that the enzymatic decomposition product is a decomposition product by a protease.

The molecular weight of the enzymatic degradation product is 2,0.
It is preferably from 00 to 70,000.

According to the present invention, it is possible to provide a skin beautifying agent capable of enhancing the water retention of the skin and obtaining a moist and fine skin by ingestion.

The skin beautifying agent of the present invention preferably further contains collagen and / or an enzymatic decomposition product thereof.
According to this aspect, the synergistic effect of collagen and / or the enzymatic decomposition product thereof and the enzymatic decomposition product of the swallow can enhance the effect of promoting beautiful skin.

The skin beautifying agent of the present invention preferably further contains at least one selected from glucosamine, nucleic acids, SOD-like substances, SOD activity inducers and lactoferrin. According to this aspect, the synergistic effect of each of the above-mentioned components and the enzymatic decomposition product of the calyx can further enhance the beautiful skin promoting effect.

The skin beautifying agent of the present invention preferably further contains at least one culture product obtained by culturing lactic acid bacteria, enterococci, yeasts or fungi. According to this aspect,
The synergistic effect with various physiologically active substances produced by the various microorganisms, such as amino acids, organic acids, vitamin B group, oligosaccharides, peptidoglycan, nucleic acid, etc., can further enhance the skin beautifying effect.

[0019]

BEST MODE FOR CARRYING OUT THE INVENTION The swallow is a nest in which swallows make their saliva into filaments, and its components contain a large amount of glycoproteins and almost no lipids.

Generally, commercially available swallows, from those which are simply cleaned by removing dirt such as hair and feces, to those which are collected by collecting dust from the swallows and molded by repeating bleaching and washing,
Although there are various types, it is preferable to use the swallows used in the present invention that have not been excessively washed or bleached in the pretreatment.

The enzymatic decomposition product of Tsubame, which is an active ingredient of the skin beautifying agent of the present invention, is obtained by decomposing Tsubame or a processed product thereof (for example, an aqueous extract of Tsubaki) with protease or a complex enzyme containing protease. Can be obtained by

The enzymatic decomposition product of the swallow can be prepared, for example, as follows. That is, 10 to 50 times the mass of water is added to the crushed swallow to swell it, and then heat treatment is performed at 60 to 130 ° C. for 5 seconds to 30 minutes.
Then, an appropriate amount of enzyme is added, and the enzyme reaction is performed at the optimum pH and the optimum temperature of the enzyme for 0.5 to 48 hours. After the completion of the enzymatic reaction, the enzyme is inactivated by heat treatment or the like, and the reaction solution is filtered to remove insoluble matter, whereby an enzymatic decomposition product of the swallow can be obtained. In addition, this enzymatic decomposition product may be dried and powdered if necessary. In each of the above steps, operations such as pH adjustment, decolorization, deodorization and the like may be appropriately performed.

On the other hand, in the case of enzymatically decomposing the water extract of the swallow, 1 to 60 ° C. is added to the swallow crushed to a particle size of 2 mm or less, preferably 150 μm or less, by adding 10 to 100 times its mass of water. After standing still for 0.5 to 48 hours or stirring to perform extraction, heat treatment is performed at 60 to 130 ° C. for 5 seconds to 30 minutes, and if necessary, using a water extract obtained by filtration, The enzyme treatment may be performed in the same manner as above.

The molecular weight of the enzyme-decomposed product of the calyx obtained in this manner is preferably 500 to 200,000,
More preferably, it is from 00 to 70,000.

The enzyme-decomposed product of the calyx obtained as described above can be used as it is as the skin beautifying agent of the present invention. Further, excipients, sweeteners, flavors, colorants and the like can be further added to give granules, tablets, capsules and the like.

The effective ingestion amount of the skin beautifying agent of the present invention is 10 to 5,000 mg as an enzyme decomposition product (converted to dry matter) of the swallow.
/ One person a day. Therefore, the content of the enzyme decomposed product of the above-mentioned crevice in the skin beautifying agent of the present invention is preferably such that the enzyme decomposed product can be ingested in an amount of 10 to 5,000 mg / day in terms of dry matter, and 50 to 500 mg can be ingested. Is more preferable.

The skin beautifying agent of the present invention preferably further contains collagen and / or an enzymatic decomposition product thereof. Collagen can be used without particular limitation as long as it has a quality that can be used for food and drink. From the viewpoint of digestion and absorbability, enzymatic degradation products of collagen are more preferably used. The enzymatic decomposition product of collagen can be prepared by decomposing collagen with an enzyme such as protease. The conditions for enzymatic degradation are not particularly limited, and the optimum p of the enzyme used is
It suffices to carry out the enzymatic reaction at H at an optimum temperature. Commercially available enzymatic degradation products of collagen may be used, and for example, “water-soluble collagen peptide PA” (trade name, manufactured by Kyowa Hakko Kogyo Co., Ltd.) and the like can be used.

The content of collagen (as dry matter) in the skin beautifying agent of the present invention is preferably 0.1 to 10 g, and more preferably 1 to 6 g per day.

Collagen has been conventionally used in beauty and health foods, and a synergistic skin beautifying effect can be expected when it is used in combination with an enzymatic decomposition product of the swallow.

Furthermore, the skin beautifying agent of the present invention may contain at least one selected from glucosamine, nucleic acids, SOD-like substances, SOD activity inducers and lactoferrin.

Glucosamine is a natural amino sugar, which is widely distributed as a constituent component of mucopolysaccharides such as cartilage and connective tissue in the body of an animal, and when used in combination with an enzymatic decomposition product of the pit, synergistic skin beautiful effects are obtained. Can be expected.

The content of glucosamine (as dry matter) in the skin beautifying agent of the present invention is preferably 0.5 g or more per day, more preferably 1.0 to 2.0 g.

The nucleic acid can be used without particular limitation as long as it is one generally used in foods. For example, a nuclear protein derived from white salmon such as salmon is treated with acid, alkali or enzyme (for example, trade name). "DNA na", manufactured by Nissan Chemical Industries, Ltd.), a brewer's yeast-derived nuclear protein that is decomposed with an acid and an alkali (for example, trade name "yeast ribonucleic acid", manufactured by Kirin Brewery Co., Ltd.) and the like. To be

Nucleic acid is known to have a skin anti-aging effect, and a synergistic skin beautifying effect can be expected by using it in combination with an enzyme decomposition product of the pit.

The content of nucleic acid (as dry matter) in the skin beautifying agent of the present invention is preferably 0.5 to 2.0 g, more preferably 1.0 to 1.5 g per day.

The SOD-like substance means an antioxidant substance having a superoxide desmutase-like activity, for example, β
-Carotene, vitamin E, vitamin C and the like.

Further, the SOD activity inducer means a substance having an action of enhancing SOD-like activity in the living body by ingestion, and for example, "oxycaine" (trade name, sold by an extract of melon) is used as a raw material. : Combi Corporation)
Etc.

By ingesting an SOD-like substance or an SOD activity inducer, free radicals generated in the living body can be eliminated. Therefore, when used in combination with the enzymatic decomposition product of the swallow, a synergistic skin-beautifying effect can be expected.

The content of the SOD-like substance in the skin beautifying agent of the present invention is, for example, in the case of β-carotene, 1 per day.
It is preferably contained so that 0 mg or more, more preferably 15 to 30 mg can be ingested. Further, in the case of vitamin E, 100 to 1,000 mg per day, more preferably 30
It is preferably contained so that 0 to 700 mg can be ingested.
In the case of vitamin C, 50 to 3,000m per day
g, more preferably 100 to 2,000 mg so that it can be ingested.

The SOD in the skin beautifying agent of the present invention
Content of activity inducer is 5-1,000m per day
g, more preferably 100 to 500 mg so that it can be ingested.

Lactoferrin is an iron-binding glycoprotein having a molecular weight of about 80,000 contained in milk of mammals. Lactoferrin has an effect of moisturizing the skin when orally ingested, and a synergistic skin beautifying effect can be expected by using it in combination with an enzymatic decomposition product of the swallow.

The content of lactoferrin (on a dry matter basis) in the skin beautifying agent of the present invention is preferably 0.5 g or more per day, more preferably 1 to 1.5 g.

Furthermore, the skin beautifying agent of the present invention may contain at least one culture product obtained by culturing lactic acid bacteria, enterococci, yeasts or fungi.

Examples of the lactic acid bacteria include Lactobacillus bulg
aricus , Lactobacillus casei , Lactobacillus acidoph
ilus , Lactobacillus lactis , Streptococcus thermoph
ilus and the like are preferred. The Enterococcus is Enterococcus
faecalis and the like are preferable. As the above yeast, Saccharom
yces serevisiae and the like are preferable. As the above fungus, Asp
ergills oryzae , Aspergills sojae and the like are preferable.

Further, examples of the medium for culturing the above-mentioned microorganism include the following mediums.

The medium for culturing lactic acid bacteria or enterococci is preferably a medium containing 0.5% by mass of yeast extract, 1% by mass of glucose and 1% by mass of polypeptone.

As a medium for culturing yeast, for example, a medium containing 20% by mass of rice leachate and 0.5% by mass of yeast extract is preferable.

As a medium for culturing a fungus, for example, a medium containing 20% by mass of potato leachate, 2% by mass of glucose and 0.5% by mass of yeast extract is preferable.

The potato leachate described above was grated from 20 to 100 g of peeled potatoes, water was added to this, and the mixture was boiled for 1 hour and strained with a cloth, and then water was added to 1,000 m.
This is L. The rice leachate was prepared by adding water to 20 g of rice, boiling it for 1 hour, filtering it with a cloth, and then adding water.
It is set to 000 mL.

In the present invention, the culture medium in which each microorganism is cultured is sterilized and filtered using the above-mentioned medium,
It is possible to use an appropriately concentrated product, or a product obtained by drying or powdering it. Such a microorganism culture contains various physiologically active substances such as amino acids, organic acids, vitamin B group, oligosaccharides (galacto-oligosaccharides, fructooligosaccharides, etc.), peptidoglycan, nucleic acids, etc. When used in combination with an enzymatic degradation product, a synergistic effect of promoting beautiful skin can be expected. The culture conditions are
It may be set appropriately according to the microorganism to be cultured.

The content of the above culture (dry matter conversion) in the skin beautifying agent of the present invention is preferably 10 g or more, more preferably 20 to 40 g per day.

The skin beautifying agent of the present invention may be taken as it is as a drug, a dietary supplement, or a beverage,
It may be ingested by adding it to various foods and drinks such as jelly, gummy candy, candy, gum, snacks, baked goods, retort foods and instant foods.

The amount of the skin beautifying agent of the present invention to be added to the food or drink is preferably 10 to 5,000 mg, in terms of dry matter, of the enzyme decomposition product of the swallow pit per serving of the food or drink, and more preferably 5.
It is 0 to 500 mg.

If the amount of the enzyme-decomposed product of Tsubame to the food or drink is out of the above range, there is a problem that a sufficient skin beautifying effect cannot be obtained or the cost becomes too high.

[0055]

EXAMPLES The present invention will be specifically described below with reference to examples.

Example Commercially available unbleached swallows were crushed by a crusher to obtain a swallow powder of 100 mesh pass (particle size 150 μm or less), and about 50 times (mass) amount of water was added to this swallow powder. 3 at 5 ℃
After swelling for 24 hours, it was heat-treated at 121 ° C. for 15 minutes.

After cooling this treatment liquid, the pH was adjusted,
2% by mass of protease-containing enzyme (trade name "Pancreatin F", manufactured by Amano Pharmaceutical Co., Ltd.) was added to the swallow powder, and the mixture was reacted at 45 ° C for 3 hours.

The pH of this reaction solution was adjusted to 7.0 and the temperature was adjusted to 90 ° C.
The mixture was heated for 5 minutes to deactivate the enzyme, and then filtered to collect the filtrate. After concentrating this filtrate, it was freeze-dried to obtain an enzymatic decomposition product of a swallow.

Test Example / Test Sample: Granular preparation (1.5 g / pack) containing 24% by mass of the enzymatic decomposition product of the swallow obtained in the Example and other excipients, etc. Obtained in the Example Granule-shaped preparation (1.5 g / pack) containing 24% by mass of enzymatic decomposition product of swallow, 42.94% by mass of enzymatic decomposition product of collagen, and 42.94% by mass of enzymatic decomposition product of collagen %, And a granular preparation containing 1.5% and other excipients (1.5 g / pack) As the enzymatic decomposition product of collagen, "water-soluble collagen peptide PA" (trade name, Kyowa Hakko Kogyo Co., Ltd.) (Manufactured by the company) was used. In addition, each test sample contained reduced maltose syrup, dextrin, stevia, and calcium phosphate in the same proportion as an excipient, and erythritol was used for adjusting the mass.

Test method: healthy women in their 20s to 50s 9
Names are Test Group 1 (enzyme hydrolyzate administration group of Tsubame), Test Group 2
The test sample was divided into (enzyme-decomposed product of swallows + enzyme-decomposed product of collagen) and comparative group (enzyme-decomposed product of collagen), and each test sample was ingested continuously for 4 days / pack (6 g / day) for 56 days. received.

Evaluation method: A replica of the skin surface of the subject was collected before the start of the test (T0) and at the end of the test (T2). In addition, the replica collection is based on the replica collection kit Dermotest "DIAGNOSKIN" (brand name, B
IO-EC Laboratory Co., Ltd.). Specifically, a Delmotest thin piece with a circular adhesive part was applied to the surface of the skin on the inner part of the forearm for about 3 times.
Lightly pressure-bonded for 0 seconds, and a replica of the skin surface was collected on the adhesive portion.

Each replica was inspected and photographed with an optical microscope, and these replicas were evaluated based on the evaluation criteria shown in Table 1. This evaluation standard is BIO-E.
It is a score based on the database of C Laboratory.

[0063]

[Table 1]

The results are shown in Table 2. The score in the table means that the higher the score, the higher the moisture retention of the skin and the better the skin condition, and the lower the score, the higher the dryness of the skin and the poorer skin condition. To do.

[0065]

[Table 2]

From the results of Table 2, the test groups 1 and 2 are
It can be seen that the skin condition tends to be improved as compared with the comparison group. In particular, it can be seen that the degree of improvement of the skin condition is large in the test group 2 ingesting the enzymatic decomposition product of the swallow and the enzymatic decomposition product of collagen.

Optical micrographs of a skin replica of each subject are shown in FIGS. 1 to 3 are test group 1 and FIG.
6 to 6 are for the test group 2 and FIGS. 7 to 9 are for the comparison group. In addition, (a) is a replica before the test (T0), and (b) is a replica at the end of the test (T2).

In FIGS. 1 to 6 (test groups 1 and 2), the state at the end of the test had less desquamation and the skin condition was improved compared to the state before the test. I understand. On the other hand, in FIGS. 7 to 9 (comparative group), it can be seen that almost no change is observed between the state before the test and the state at the end of the test.

[0069]

As described above, according to the present invention, it is possible to improve the moisturization of the skin and to obtain a moist and fine skin by containing the enzymatic decomposition product of the swallow pit as an active ingredient. A skin beautifying agent can be provided.

[Brief description of drawings]

1] Subject No. It is a figure which shows the microscope picture of the replica of 1 skin.

2] Subject No. It is a figure which shows the microscope picture of the replica of the skin of 2.

3] Subject No. It is a figure which shows the microscope picture of the replica of the skin of FIG.

4] Subject No. 4 is a view showing a micrograph of a skin replica of FIG.

5] Subject No. 5 is a view showing a micrograph of a skin replica of FIG.

6] Subject No. 6 is a view showing a micrograph of a skin replica of No. 6; FIG.

7] Subject No. It is a figure which shows the micrograph of the replica of the skin of FIG.

FIG. 8: Subject No. 8 is a view showing a micrograph of a skin replica of FIG.

9] Subject No. FIG. 9 shows a micrograph of a skin replica of No. 9.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 35/70 A61K 35/70 35/72 35/72 35/74 35/74 G 38/16 45/00 38/17 A61P 17/16 45/00 A61K 37/12 A61P 17/16 37/14 F term (reference) 4B018 LB08 LB10 MD20 MD23 MD27 MD44 MD69 MD80 MD81 MD85 ME14 MF12 4C084 AA02 AA19 AA22 BA43 MA02 MA52 NA14 ZA891 4C086 AA01 AA02 EA02 EA16 MA03 MA52 NA14 ZA89 4C087 AA01 AA02 BB33 BC01 BC11 BC55 MA02 MA52 NA14 ZA89

Claims (6)

[Claims] 1. A skin beautifying agent comprising an enzyme decomposition product of a swallow as an active ingredient. 2. The skin beautifying agent according to claim 1, wherein the enzymatic decomposition product is a decomposition product of a protease. 3. The enzymatic decomposition product has a molecular weight of 2,000.
The skin beautifying agent according to claim 1 or 2, which is ˜70,000. 4. The skin beautifying agent according to claim 1, further comprising collagen and / or an enzymatic decomposition product thereof. 5. The skin beautifying agent according to claim 1, further comprising at least one selected from glucosamine, nucleic acid, SOD-like substance, SOD activity inducer, and lactoferrin. 6. The skin beautifying agent according to claim 1, further comprising at least one culture product obtained by culturing lactic acid bacteria, enterococci, yeasts or fungi.