CN110157758A - The purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation - Google Patents
The purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation Download PDFInfo
- Publication number
- CN110157758A CN110157758A CN201711436406.4A CN201711436406A CN110157758A CN 110157758 A CN110157758 A CN 110157758A CN 201711436406 A CN201711436406 A CN 201711436406A CN 110157758 A CN110157758 A CN 110157758A
- Authority
- CN
- China
- Prior art keywords
- flos rosae
- rosae rugosas
- rugosas pollen
- fermentation
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000628997 Flos Species 0.000 title claims abstract description 77
- 241000698291 Rugosa Species 0.000 title claims abstract description 77
- 238000000855 fermentation Methods 0.000 title claims abstract description 71
- 230000004151 fermentation Effects 0.000 title claims abstract description 71
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 54
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 47
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 27
- 239000000243 solution Substances 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 23
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 19
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 19
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 19
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 15
- 239000002537 cosmetic Substances 0.000 claims abstract description 12
- 230000036541 health Effects 0.000 claims abstract description 12
- 235000013373 food additive Nutrition 0.000 claims abstract description 11
- 239000002778 food additive Substances 0.000 claims abstract description 11
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 11
- 239000007853 buffer solution Substances 0.000 claims abstract description 7
- 238000003860 storage Methods 0.000 claims abstract description 6
- 239000012047 saturated solution Substances 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 238000000605 extraction Methods 0.000 claims description 18
- 239000000284 extract Substances 0.000 claims description 14
- 239000006228 supernatant Substances 0.000 claims description 14
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 241000220317 Rosa Species 0.000 claims description 13
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 10
- 239000000047 product Substances 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 6
- 238000004090 dissolution Methods 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 5
- 230000009849 deactivation Effects 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000005360 mashing Methods 0.000 claims description 5
- 230000001376 precipitating effect Effects 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 2
- 239000005864 Sulphur Substances 0.000 claims description 2
- MIOPJNTWMNEORI-UHFFFAOYSA-N camphorsulfonic acid Chemical class C1CC2(CS(O)(=O)=O)C(=O)CC1C2(C)C MIOPJNTWMNEORI-UHFFFAOYSA-N 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 241000919496 Erysimum Species 0.000 claims 1
- 238000000527 sonication Methods 0.000 claims 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 16
- 238000010521 absorption reaction Methods 0.000 abstract description 10
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 9
- 239000011575 calcium Substances 0.000 abstract description 9
- 229910052791 calcium Inorganic materials 0.000 abstract description 9
- 230000003078 antioxidant effect Effects 0.000 abstract description 8
- 229910052742 iron Inorganic materials 0.000 abstract description 8
- 150000003254 radicals Chemical class 0.000 abstract description 8
- 230000036039 immunity Effects 0.000 abstract description 7
- 238000001727 in vivo Methods 0.000 abstract description 7
- 230000002708 enhancing effect Effects 0.000 abstract description 6
- 238000005502 peroxidation Methods 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 238000002137 ultrasound extraction Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- MIOPJNTWMNEORI-GMSGAONNSA-N (S)-camphorsulfonic acid Chemical compound C1C[C@@]2(CS(O)(=O)=O)C(=O)C[C@@H]1C2(C)C MIOPJNTWMNEORI-GMSGAONNSA-N 0.000 description 5
- 239000005515 coenzyme Substances 0.000 description 5
- 230000007071 enzymatic hydrolysis Effects 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 238000009210 therapy by ultrasound Methods 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 3
- 125000003118 aryl group Chemical group 0.000 description 3
- 230000003796 beauty Effects 0.000 description 3
- 235000019658 bitter taste Nutrition 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 238000005374 membrane filtration Methods 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 229920006395 saturated elastomer Polymers 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 235000019640 taste Nutrition 0.000 description 3
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 238000002525 ultrasonication Methods 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 241000723346 Cinnamomum camphora Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 206010013935 Dysmenorrhoea Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 208000037093 Menstruation Disturbances Diseases 0.000 description 1
- 206010027339 Menstruation irregular Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- -1 carrotene Chemical compound 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000035613 defoliation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 230000003617 peroxidasic effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Genetics & Genomics (AREA)
- Public Health (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Dermatology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Nutrition Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Cosmetics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation, Flos Rosae Rugosas pollen polypeptide can be used for food additives, cosmetics, in health care product, smash rose to pieces rear cold storage, it is then dissolved in PBS buffer solution, broken pollen solution is obtained after ultrasonic extraction, it is separately added into the protein that ammonium sulfate is configured to the ammonium sulfate saturated solution of different gradient concentrations and collects precipitation, bacillus subtilis seed liquor is inoculated in the fermentation culture containing Flos Rosae Rugosas pollen protein and carries out fermented and cultured, fermentation liquid is obtained into Flos Rosae Rugosas pollen polypeptide after ultrafiltration.Have the beneficial effect that Flos Rosae Rugosas pollen protein peptides are easily absorbed by the body, it can promote absorption of the human body to iron and calcium, Flos Rosae Rugosas pollen polypeptide also has stronger antioxidant activity, it can remove free radical extra in vivo, anti-lipid peroxidation reaction, it is effective to promote body immunity, enhancing skin vigor and elasticity, suitable for food additives, cosmetics, health care product.
Description
Technical field
The present invention relates to pollen protein peptide arts, more particularly, to the use of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation
On the way.
Technical background
Rose, alias are hovered flower, dahurian rose flower, nib flower, are rosaceae Rosa defoliation bush plants, rosette is tender
Beauty, faint scent is simple and elegant, various colors.Rose mainly contains vitamin C, carrotene, thiamine, riboflavin, amaroid, tannin
And the minerals such as potassium, sodium, calcium, magnesium.Rose in vitamin C be content be 50 times of lemon.Human body generation can be enhanced
The ability of collagen, resists the infringement of free radical, and makes skin from uv damage.Rose is warm-natured, sweet in flavor, Xin Xiang
Slight bitter can help internal healthy tendency to run, and push blood circulation, menstruation regulating of becoming silted up of dispelling.Modern scientific research discovery, rose
Contained volatile oil plays the role of promoting bile secretion, can help the digestion of stomach.Roseleaf can both take a shower or skin-protecting face nursing, is
New century beauty and skin care good merchantable brand.During the annual 5-6 month, when rose will open, its fresh and tender bud is won in batches, is passed through
Stringent disinfection, air-dries sterilizing, and bud fragrance is pleasant, there is the work such as qi-regulating and blood, liver soothing and depression relief, lower blood-fat and reduce weight, moisturizing beauty treatment
With.Especially it is of miraculous efficacy to women's dysmenorrhoea, irregular menstruation.Taking it can play the role of enriching blood fostering the spirit of nobility, and nourish appearance.
Extractable attar of rose is spent, dried flower Lei Genke is used as medicine, and it is fiery, beautifying face and moistering lotion peculiar to have effects that heat-clearing disappears.
Summary of the invention
The purpose of the present invention is to provide the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation, Flos Rosae Rugosas pollen albumen is more
Peptide can promote absorption of the human body to other nutrients such as iron and calcium, also have stronger antioxidant activity, can be applied to food
Additive, cosmetics, health care product.
Used microorganism and its bought enterprise in the method for the present invention are as follows: bacillus subtilis, Wuhan Feng Tian biology
Science and Technology Ltd..
The present invention in background technique aiming at the problem that mentioning, the technical solution taken are as follows: the Flos Rosae Rugosas pollen of fermentation method preparation
The purposes of polypeptide, Flos Rosae Rugosas pollen polypeptide can be used for food additives, cosmetics, in health care product, fermentation method preparation
Flos Rosae Rugosas pollen polypeptide not only be easier to be absorbed by the body than Flos Rosae Rugosas pollen albumen itself, human body can also be promoted to other
The absorption of nutrient such as iron and calcium, it is often more important that, Flos Rosae Rugosas pollen polypeptide also has stronger antioxidant activity, it can be with
Free radical extra in vivo is removed, anti-lipid peroxidation reaction can effectively promote body immunity, enhance skin vigor
With elasticity, therefore suitable for food additives, cosmetics, health care product.
Preferably, the method for the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation includes: broken wall treatment, extracts albumen, hair
Ferment, extraction, specifically includes the following steps:
Broken wall treatment: by rose with high-speed tissue mashing machine crush in -18~-20 DEG C cold storage 12-24 hours, by solid-liquid ratio
1g:10-12mL is added to 78-80 DEG C of PBS buffer solution, and the concentration of buffer is 0.1mol/L, pH 7.4, and stirring is straight rapidly
It is dissolved to pollen, ultrasonic treatment then is carried out 15-25 minutes to pollen solution, is then cooled to 40-45 DEG C rapidly, and use water-bath
Heat preservation 6-8 hours, 0-4 DEG C of stirring are extracted 10-12 hours, and broken wall solution is taken to be stored in 0-4 DEG C of environment；Flos Rosae Rugosas pollen is surpassed
Sound broken wall treatment can effectively separate protein and lipid, the cellulose etc. in pollen, significantly more efficient can mention
Take Flos Rosae Rugosas pollen albumen；
It extracts albumen: broken pollen solution is taken into supernatant after low-speed centrifugal 15-20 minutes under 4800-5000r/min revolving speed,
It is 20% that ammonium sulfate to saturation degree is added at a temperature of 3-4 DEG C in supernatant, stands 2-3 hours after dissolution, 3-4 DEG C, 10000-
Above-mentioned solution is centrifuged 10-12 minutes under the conditions of 12000r/min, takes precipitating, ammonium sulfate is added in clear liquid respectively to saturation degree
Aforesaid operations are repeated after being 40%, 60%, 80%, 100%, can successively obtain ammonium sulfate saturation degree is 0-20%, 20-40%, 40-
60%, the water soluble protein of 60-80%, 80-100%, low temperature drying is after protein is merged up to Flos Rosae Rugosas pollen albumen；More sulphur
Sour ammonium saturated concentration, which is extracted, can sufficiently, thoroughly extract the albumen in pollen, realize to the complete of Flos Rosae Rugosas pollen albumen
Effect utilizes, and extraction efficiency is higher；
Fermentation: bacillus subtilis seed liquor is linked into fermentation culture by the 5-11% of nutrient solution volume, in seed liquor
Viable count is 1.0-1.2 × 108A/mL is 30-41 DEG C in temperature, under conditions of shaking speed is 180-200r/min, water-bath
Ferment at constant temperature culture 9-20 hours, after fermentation at boiling water bath enzyme deactivation 10-15 minutes；Fermentation energy is by microorganism producing enzyme and enzyme water
Solution reaction carries out simultaneously, reduces costs, and the raw peptide ending enzyme of producing bacillus subtilis to Flos Rosae Rugosas pollen protein peptides with repairing
Decorations effect makes peptide obtained not only without bitter taste, also has the natural aromatic taste of fermentation；Flos Rosae Rugosas pollen protein peptides are relative to albumen
Molecular weight is lower for matter, it is easier to be absorbed by the body, can also promote absorption of the human body to other nutrients such as iron and calcium, more
Importantly, Flos Rosae Rugosas pollen polypeptide also has stronger antioxidant activity, it can remove free radical extra in vivo, suppression
Peroxidatic reaction of lipid processed can effectively promote body immunity, enhancing skin vigor and elasticity, therefore be suitable for food additive
Add agent, cosmetics, in health care product；
It extracts: fermentation liquid being centrifuged 20-30 minutes under 5000-5500r/min revolving speed, takes supernatant, with 0.45 μm of filter membrane mistake
Filter, then with 10kDa ultrafiltration membrane ultrafiltration, takes filtrate low temperature drying up to Flos Rosae Rugosas pollen polypeptide；It can be with by fermentation liquid centrifugation
Thallus and unfermentable Flos Rosae Rugosas pollen residue are removed, this easy extraction is low in cost, and extraction efficiency is higher.
Preferably, taking 10.0-10.2 parts of Flos Rosae Rugosas pollen albumen, 0.25-0.28 parts of glucose, 0.55-0.58 parts of chlorinations
Sodium, 0.01-0.012 parts of 10- camphorsulfonic acids are dissolved in 150-180 parts of distilled water up to fermentation culture, adjust pH value to
6.7-6.8 sterilizes 20-25 minutes at 115-121 DEG C, takes out, and is cooled to room temperature stand-by；Flos Rosae Rugosas pollen albumen both serves as nitrogen source
But also as the substrate of microorganism enzymatic hydrolysis, rapid fermentation and the enzymatic hydrolysis of microorganism is may be implemented in fermentation culture；Micro 10- camphor tree
Brain sulfonic acid can act on the metal active centres of the portion of Coenzyme of bacillus subtilis, related auxiliary so as to effectively improve
The activity of enzyme promotes the progress of bacillus subtilis tricarboxylic acid cycle, and then enhances the metabolic activity of bacillus subtilis, improves
Fermentation efficiency reduces fermentation time, improves yield and yield.
Compared with the prior art, the advantages of the present invention are as follows: 1) Flos Rosae Rugosas pollen powder both served as nitrogen source but also as micro- life
Rapid fermentation and the enzymatic hydrolysis of microorganism may be implemented in the substrate of object enzymatic hydrolysis, fermentation culture；Micro 10- camphorsulfonic acid can be made
The metal active centres of portion of Coenzyme for bacillus subtilis promote so as to effectively improve the activity of associated coenzymes
Into the progress of bacillus subtilis tricarboxylic acid cycle, and then enhance the metabolic activity of bacillus subtilis, improves fermentation efficiency, drop
Low fermentation time improves yield and yield；2) Flos Rosae Rugosas pollen protein peptides molecular weight for protein is lower, it is easier to quilt
Absorption of human body can also promote absorption of the human body to other nutrients such as iron and calcium, it is often more important that, Flos Rosae Rugosas pollen polypeptide
Also there is stronger antioxidant activity, it can remove free radical extra in vivo, and anti-lipid peroxidation reaction can be effective
Promotion body immunity, enhancing skin vigor and elasticity, therefore suitable for food additives, cosmetics, health care product.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
The method of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation, comprising the following steps:
1) by rose with high-speed tissue mashing machine crush in -18 DEG C cold storage 12 hours, be added to 78 DEG C by solid-liquid ratio 1g:10mL
PBS buffer solution, the concentration of buffer is 0.1mol/L, pH 7.4, and stirring is then molten to pollen until pollen dissolves rapidly
Liquid carries out ultrasonic treatment 15 minutes, is then cooled to 40 DEG C rapidly, and keep the temperature 6 hours with water-bath, and 0 DEG C of stirring is extracted 10 hours,
Broken wall solution is taken to be stored in 0 DEG C of environment；2) by broken pollen solution, low-speed centrifugal takes after 15 minutes under 4800r/min revolving speed
Clear liquid, it is 20% that ammonium sulfate to saturation degree is added at a temperature of 3 DEG C in supernatant, stands 2 hours after dissolution, 3 DEG C, 10000r/
Under the conditions of min by above-mentioned solution be centrifuged 10 minutes, take precipitating, in clear liquid be added ammonium sulfate respectively to saturation degree be 40%, 60%,
80%, aforesaid operations are repeated after 100%, can successively obtain ammonium sulfate saturation degree be 0-20%, 20-40%, 40-60%, 60-80%,
The water soluble protein of 80-100%, low temperature drying is after protein is merged up to Flos Rosae Rugosas pollen albumen；3) fermentation culture contains
10.0 parts of Flos Rosae Rugosas pollen albumen, 0.25 part of glucose, 0.55 part of sodium chloride, 0.01 part of 10- camphorsulfonic acid, 150 parts of distilled water are adjusted
PH value is saved to 6.7, is sterilized 20 minutes at 115 DEG C, is taken out, is cooled to room temperature stand-by；By nutrient solution volume 5% by withered grass bud
Spore bacillus seed liquor is linked into fermentation culture, and the viable count in seed liquor is 1.0 × 108A/mL, in 30 DEG C, 180r/
Under the conditions of min, constant temperature water bath fermented and cultured 9 hours, after fermentation at boiling water bath enzyme deactivation 10 minutes；4) fermentation liquid is existed
It is centrifuged 20 minutes under 5000r/min revolving speed, takes supernatant, taken with 0.45 μm of membrane filtration then with 10kDa ultrafiltration membrane ultrafiltration
Filtrate low temperature drying is up to Flos Rosae Rugosas pollen polypeptide；Fermentation liquid centrifugation can be removed into thallus and unfermentable Flos Rosae Rugosas pollen is residual
Slag, this easy extraction is low in cost, and extraction efficiency is higher.
Embodiment 2:
The method of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation includes: broken wall treatment, extracts albumen, fermentation, extraction, specific to wrap
Include following steps:
1) broken wall treatment: by rose with high-speed tissue mashing machine crush in -20 DEG C cold storage 24 hours, by solid-liquid ratio 1g:12mL
It is added to 80 DEG C of PBS buffer solution, the concentration of buffer is 0.1mol/L, pH 7.4, and stirring is until pollen dissolves, so rapidly
Ultrasonic treatment is carried out 25 minutes to pollen solution afterwards, is then cooled to 45 DEG C rapidly, and keep the temperature 8 hours with water-bath, 4 DEG C of stirrings mention
It takes 12 hours, broken wall solution is taken to be stored in 4 DEG C of environment；Carrying out ultrasonication processing to Flos Rosae Rugosas pollen can effectively will be in pollen
Protein and lipid, cellulose etc. separate, can significantly more efficient extraction Flos Rosae Rugosas pollen albumen；
2) extract albumen: by broken pollen solution, low-speed centrifugal takes supernatant, 4 DEG C of temperature after twenty minutes under 5000r/min revolving speed
It is 20% that ammonium sulfate to saturation degree is added under degree in supernatant, stands 3 hours after dissolution, 4 DEG C, general under the conditions of 12000r/min
Above-mentioned solution is centrifuged 12 minutes, takes precipitating, after addition ammonium sulfate is respectively 40%, 60%, 80%, 100% to saturation degree in clear liquid
Aforesaid operations are repeated, the water that ammonium sulfate saturation degree is 0-20%, 20-40%, 40-60%, 60-80%, 80-100% can be successively obtained
Soluble proteins, low temperature drying is after protein is merged up to Flos Rosae Rugosas pollen albumen；More ammonium sulfate saturated concentrations, which are extracted, to incite somebody to action
Albumen in pollen sufficiently, thoroughly extracts, and realizes and utilizes to the full effect of Flos Rosae Rugosas pollen albumen, extraction efficiency is higher；
3) it ferments: taking 10.2 parts of Flos Rosae Rugosas pollen albumen, 0.28 part of glucose, 0.58 part of sodium chloride, 0.012 part of 10- camphorsulfonic acid
It is dissolved in 180 parts of distilled water up to fermentation culture, adjusts pH value to 6.8, sterilize 25 minutes at 121 DEG C, take out, it is cooling
It is stand-by to room temperature；Bacillus subtilis seed liquor is linked into fermentation culture by the 11% of nutrient solution volume, in seed liquor
Viable count is 1.2 × 108A/mL, under the conditions of 41 DEG C, 200r/min, constant temperature water bath fermented and cultured 20 hours, after fermentation
At boiling water bath enzyme deactivation 15 minutes；Fermentation energy carries out microorganism producing enzyme with enzymatic hydrolysis reaction simultaneously, reduces costs, and withered grass
The peptide ending enzyme that bacillus generates has modification to Flos Rosae Rugosas pollen protein peptides, makes peptide obtained not only without bitter taste, also has
There is the natural aromatic taste of fermentation；Flos Rosae Rugosas pollen protein peptides molecular weight for protein is lower, it is easier to it is absorbed by the body,
Can also promote absorption of the human body to other nutrients such as iron and calcium, it is often more important that, Flos Rosae Rugosas pollen polypeptide also have compared with
Strong antioxidant activity, it can remove free radical extra in vivo, and anti-lipid peroxidation reaction can effectively promote people
Body immunity, enhancing skin vigor and elasticity, therefore suitable for food additives, cosmetics, health care product；
4) it extracts: fermentation liquid being centrifuged 30 minutes under 5500r/min revolving speed, takes supernatant, with 0.45 μm of membrane filtration, then
With 10kDa ultrafiltration membrane ultrafiltration, take filtrate low temperature drying up to Flos Rosae Rugosas pollen polypeptide；Fermentation liquid centrifugation can be removed into thallus
With unfermentable Flos Rosae Rugosas pollen residue, this easy extraction is low in cost, and extraction efficiency is higher.
Embodiment 3:
The method of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation includes: broken wall treatment, extracts albumen, fermentation, extraction, specific to wrap
Include following steps:
Broken wall treatment: by rose with high-speed tissue mashing machine crush in -20 DEG C cold storage 15 hours, add by solid-liquid ratio 1g:10mL
Enter the PBS buffer solution to 78 DEG C, the concentration of buffer is 0.1mol/L, pH 7.4, and stirring is until pollen dissolves, then rapidly
Ultrasonic treatment is carried out 20 minutes to pollen solution, is then cooled to 42 DEG C rapidly, and keep the temperature 7 hours with water-bath, 2 DEG C of stirrings are extracted
10 hours, broken wall solution is taken to be stored in 2 DEG C of environment；Carrying out ultrasonication processing to Flos Rosae Rugosas pollen can effectively will be in pollen
Protein and lipid, cellulose etc. separate, can significantly more efficient extraction Flos Rosae Rugosas pollen albumen；
Extract albumen: by broken pollen solution, low-speed centrifugal takes supernatant, 3 DEG C of temperature under 4800r/min revolving speed after 16 minutes
Under ammonium sulfate to saturation degree is added in supernatant is 20%, stands 2.5 hours after dissolution, 3 DEG C, general under the conditions of 11000r/min
Above-mentioned solution is centrifuged 10 minutes, takes precipitating, after addition ammonium sulfate is respectively 40%, 60%, 80%, 100% to saturation degree in clear liquid
Aforesaid operations are repeated, the water that ammonium sulfate saturation degree is 0-20%, 20-40%, 40-60%, 60-80%, 80-100% can be successively obtained
Soluble proteins, low temperature drying is after protein is merged up to Flos Rosae Rugosas pollen albumen；More ammonium sulfate saturated concentrations, which are extracted, to incite somebody to action
Albumen in pollen sufficiently, thoroughly extracts, and realizes and utilizes to the full effect of Flos Rosae Rugosas pollen albumen, extraction efficiency is higher；
Fermentation: bacillus subtilis seed liquor is linked into fermentation culture by the 10% of nutrient solution volume, the work in seed liquor
Bacterium number is 1.1 × 108A/mL constant temperature water bath fermented and cultured 15 hours, exists after fermentation under the conditions of 37 DEG C, 185r/min
Boiling water bath enzyme deactivation 12 minutes；Fermentation energy carries out microorganism producing enzyme with enzymatic hydrolysis reaction simultaneously, reduces costs, and withered grass bud
The peptide ending enzyme that spore bacillus generates has modification to Flos Rosae Rugosas pollen protein peptides, makes peptide obtained not only without bitter taste, also has
The natural aromatic taste of fermentation；Flos Rosae Rugosas pollen protein peptides molecular weight for protein is lower, it is easier to be absorbed by the body, also
It can promote absorption of the human body to other nutrients such as iron and calcium, it is often more important that, Flos Rosae Rugosas pollen polypeptide also has relatively strong
Antioxidant activity, it can remove extra free radical in vivo, and anti-lipid peroxidation reaction can effectively promote human body
Immunity, enhancing skin vigor and elasticity, therefore suitable for food additives, cosmetics, health care product；
Extract: fermentation liquid being centrifuged 25 minutes under 5400r/min revolving speed, takes supernatant, with 0.45 μm of membrane filtration, then with
10kDa ultrafiltration membrane ultrafiltration, takes filtrate low temperature drying up to Flos Rosae Rugosas pollen polypeptide；By fermentation liquid centrifugation can remove thallus and
Unfermentable Flos Rosae Rugosas pollen residue, this easy extraction is low in cost, and extraction efficiency is higher.
10 parts of Flos Rosae Rugosas pollen albumen, 0.26 part of glucose, 0.56 part of sodium chloride, 0.01 part of 10- camphorsulfonic acid is taken to be dissolved in
Up to fermentation culture in 160 parts of distilled water, pH value is adjusted to 6.7, is sterilized 22 minutes at 120 DEG C, takes out, is cooled to room temperature
For use；Flos Rosae Rugosas pollen albumen had both served as the substrate that nitrogen source is digested but also as microorganism, and microorganism may be implemented in fermentation culture
It is rapid fermentation and enzymatic hydrolysis；Micro 10- camphorsulfonic acid can act on the metal active of the portion of Coenzyme of bacillus subtilis
Center promotes the progress of bacillus subtilis tricarboxylic acid cycle, Jin Erzeng so as to effectively improve the activity of associated coenzymes
The metabolic activity of strong bacillus subtilis, improves fermentation efficiency, reduces fermentation time, improves yield and yield.
The purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation, Flos Rosae Rugosas pollen polypeptide can be used for food addition
Agent, cosmetics, in health care product, the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation is not only easier quilt than Flos Rosae Rugosas pollen albumen itself
Absorption of human body can also promote absorption of the human body to other nutrients such as iron and calcium, it is often more important that, Flos Rosae Rugosas pollen polypeptide
Also there is stronger antioxidant activity, it can remove free radical extra in vivo, and anti-lipid peroxidation reaction can be effective
Promotion body immunity, enhancing skin vigor and elasticity, therefore suitable for food additives, cosmetics, health care product.
Routine operation in operation of the present invention step is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Claims (8)
1. the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation, it is characterised in that: Flos Rosae Rugosas pollen polypeptide is in food additive
Add agent, cosmetics, the application in health care product.
2. the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation according to claim 1, it is characterised in that: the hair
The preparation method of Flos Rosae Rugosas pollen polypeptide of ferment method preparation includes:
Broken wall treatment: by rose with high-speed tissue mashing machine crush in -18~-20 DEG C cold storage 12-24 hours, by solid-liquid ratio
1g:10-12mL is added to 78-80 DEG C of PBS buffer solution, and stirring is until pollen dissolution, then surpasses pollen solution rapidly
Sonication 15-25 minutes, it is then cooled to 40-45 DEG C rapidly, and keep the temperature 6-8 hours with water-bath, stirring takes broken wall flower after extracting
Powder solution is stored in 0-4 DEG C of environment；
It extracts albumen: broken pollen solution is taken into supernatant after low-speed centrifugal 15-20 minutes under 4800-5000r/min revolving speed,
It is 20% that ammonium sulfate to saturation degree is added at a temperature of 3-4 DEG C in supernatant, stands 2-3 hours after dissolution, precipitating is taken after centrifugation,
It is added in clear liquid after ammonium sulfate is respectively 40%, 60%, 80%, 100% to saturation degree and repeats aforesaid operations, can successively obtain sulphur
Sour ammonium saturation degree is the water soluble protein of 0-20%, 20-40%, 40-60%, 60-80%, 80-100%, low after protein is merged
Temperature is drying to obtain Flos Rosae Rugosas pollen albumen；
Bacillus subtilis seed liquor: being linked into fermentation culture by fermentation by the 5-11% of nutrient solution volume, constant temperature water bath hair
Ferment culture 9-20 hours, after fermentation at boiling water bath enzyme deactivation 10-15 minutes；
It extracts: fermentation liquid being centrifuged 20-30 minutes under 5000-5500r/min revolving speed, takes supernatant, with 0.45 μm of filter membrane mistake
Filter, then with 10kDa ultrafiltration membrane ultrafiltration, takes filtrate low temperature drying up to Flos Rosae Rugosas pollen polypeptide.
3. the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation according to claim 2, it is characterised in that: described broken
In wall processing step, the concentration of PBS buffer solution is 0.1mol/L, pH 7.4.
4. the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation according to claim 2, it is characterised in that: described broken
In wall processing step, the temperature for stirring extraction is 0-4 DEG C, and extraction time is 10-12 hours.
5. the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation according to claim 2, it is characterised in that: described to mention
It takes in protein step, the centrifugal rotational speed of ammonium sulfate saturated solution is 10000-12000r/min, and temperature is 3-4 DEG C, when centrifugation
Between be 10-12 minutes.
6. the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation according to claim 2, it is characterised in that: the hair
In ferment step, fermentation culture the preparation method comprises the following steps: take 10.0-10.2 parts of Flos Rosae Rugosas pollen albumen, 0.25-0.28 parts of glucose,
0.55-0.58 parts of sodium chloride, 0.01-0.012 parts of 10- camphorsulfonic acids are dissolved in 150-180 parts of distilled water up to fermented and cultured
Liquid adjusts pH value to 6.7-6.8, sterilizes 20-25 minutes, take out at 115-121 DEG C, be cooled to room temperature stand-by.
7. the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation according to claim 2, it is characterised in that: the hair
In ferment step, the viable count in seed liquor is 1.0-1.2 × 108A/mL.
8. the purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation according to claim 2, it is characterised in that: the hair
In ferment step, fermentation temperature is 30-41 DEG C, shaking speed 180-200r/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711436406.4A CN110157758A (en) | 2017-12-26 | 2017-12-26 | The purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711436406.4A CN110157758A (en) | 2017-12-26 | 2017-12-26 | The purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110157758A true CN110157758A (en) | 2019-08-23 |
Family
ID=67640877
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711436406.4A Withdrawn CN110157758A (en) | 2017-12-26 | 2017-12-26 | The purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110157758A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113413351A (en) * | 2021-07-15 | 2021-09-21 | 合肥卡迪尔生物科技有限公司 | Fermentation liquor with whitening and anti-aging effects, fermented polypeptide, and preparation method and application thereof |
| CN113667708A (en) * | 2021-08-23 | 2021-11-19 | 山东林森生物制品股份有限公司 | Preparation method of plant intercellular substance |
| CN113813209A (en) * | 2021-10-26 | 2021-12-21 | 上海百雀羚日用化学有限公司 | Rose petal fermentation liquor and preparation method and application thereof |
| CN115386611A (en) * | 2022-10-31 | 2022-11-25 | 山东睿鹰制药集团有限公司 | Peony pollen protein polypeptide and preparation method and application thereof |
-
2017
- 2017-12-26 CN CN201711436406.4A patent/CN110157758A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113413351A (en) * | 2021-07-15 | 2021-09-21 | 合肥卡迪尔生物科技有限公司 | Fermentation liquor with whitening and anti-aging effects, fermented polypeptide, and preparation method and application thereof |
| CN113413351B (en) * | 2021-07-15 | 2023-10-20 | 合肥卡迪尔生物科技有限公司 | Fermented liquid and fermented polypeptide with whitening and anti-aging effects, and preparation methods and applications thereof |
| CN113667708A (en) * | 2021-08-23 | 2021-11-19 | 山东林森生物制品股份有限公司 | Preparation method of plant intercellular substance |
| CN113813209A (en) * | 2021-10-26 | 2021-12-21 | 上海百雀羚日用化学有限公司 | Rose petal fermentation liquor and preparation method and application thereof |
| CN113813209B (en) * | 2021-10-26 | 2024-03-22 | 上海百雀羚日用化学有限公司 | Rose petal fermentation liquor and preparation method and application thereof |
| CN115386611A (en) * | 2022-10-31 | 2022-11-25 | 山东睿鹰制药集团有限公司 | Peony pollen protein polypeptide and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110157758A (en) | The purposes of the Flos Rosae Rugosas pollen polypeptide of fermentation method preparation | |
| CN1863526A (en) | Collagen productin enhancer and production process and use thereof | |
| JP4976629B2 (en) | Skin promotion agent | |
| CN107929091A (en) | A kind of extracting method of high activity collagen and its application | |
| JP2022532152A (en) | Soap composition using Yamayosan and soap containing it | |
| CN109864134A (en) | Soybean and peanut or single substance are the method that raw material produces small-molecular peptides milk and powder | |
| CN101238904A (en) | Chickpea bean sprout juice tea and preparation method and application | |
| JP7315986B2 (en) | Spray composition for improving scalp health containing Houttuynia cordata component | |
| CN108498447A (en) | A kind of plant enzyme cleaning bubble liquid preparation | |
| CN111657383A (en) | Machilus thunbergii leaf substitute tea and preparation method thereof | |
| CN101429468A (en) | Method for producing peach blossom oil | |
| CN103122294B (en) | Wine containing SOD (Superoxide Dismutase) component and preparation method thereof | |
| JP2006271235A (en) | Beauty and health beverage, and method for producing the same | |
| KR102153476B1 (en) | Hair permanent agents comprising free cysteamine | |
| CN107951825A (en) | A kind of spot-eliminating skin care product containing Chinese wolfberry fruit dry cell extract and preparation method thereof | |
| CN104206582A (en) | Aquilaria sinensis leaf tea drink and preparation technology thereof | |
| CN106867768A (en) | A kind of Pitaya Flower peach blossom health glutinous rice wine and preparation method thereof | |
| CN104206581A (en) | Aquilaria sinensis flower tea drink and preparation technology thereof | |
| CN113016901A (en) | Rose black tea and preparation method thereof | |
| KR102249711B1 (en) | Composition for enhancing skin elasticity or improving skin wrinkels comprising enzyme treated extract of leguminous plants cultured root | |
| CN102919394A (en) | Tea production technology | |
| KR20180038306A (en) | The manufacturing method rice wine containing stevia ingredients | |
| CN104642573A (en) | Process for making mulberry leaf tea | |
| KR102112963B1 (en) | a composite for dermatitis treatment and improvement using Wild Cultivated Ginseng vinegar | |
| CN106213307B (en) | A kind of method that ginger potato adornment eats homologous high-valued comprehensive utilization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190823 |