JPH01246208A - External use preparation for skin - Google Patents

Abstract

PURPOSE:To obtain external preparation for skin which has effects of preventing skins from being aged, because of its excellent effect of improving skin roughness, softening horny skins, turn over accelerating effect and wound healing acceleration, by using protein-removed thymus extract and vitamins. CONSTITUTION:The external preparation for skin contains 0.0001-5.0wt.% of proteinremoved thymus extract obtained from animal thymus and 0.0001-5.0wt.% of vitamins. The protein-removed extract is obtained by defatting thymuses from animals with ether, combining with a buffer solution of 6.8-7.9pH, hydrolyzing with an organic acid of 3 or lower pH and extracting and adding protease to the residue. The preparation for skin can contain the above-stated essential component as well as other components used usually and is used in the forms of cosmetic lotion, emulsion, cream or ointment.

Description

[Detailed Description of the Invention] [Industrial Field of Application] The present invention is characterized by blending a deproteinized thymus extract with one or more vitamins, which has an effect on improving rough skin, an effect on improving keratin, The present invention relates to a skin preparation for external use that has excellent turnover rate promoting effects, wound healing promoting effects, and prevents skin aging.

[Prior Art] Rough skin is caused by dryness, a decrease in the amount of moisture in the skin, and a decline in the function of corneocytes.

It tends to worsen especially when the air is dry in winter, and to improve this condition, it is necessary to increase skin turnover and maintain normal skin function, and various drugs have been developed. It's here.

Conventional methods include applying sebum-like compositions or moisturizers to the skin surface and covering the skin to prevent dryness, and using vitamin E preparations to promote blood circulation in the skin. , its effect is still not sufficient,
The effect was not what I expected.

[Problems to be Solved by the Invention] As a result of intensive research into a method to prevent skin aging by enhancing the effect of preventing and improving skin roughness, the present inventors found that a deproteinized thymus extract and a type of vitamin Alternatively, we found that the combination of two or more types has the effect of accelerating skin turnover, and as a result of research focusing on this, we combined deproteinized thymus extract and one or more vitamins. The present inventors have discovered that when administered transdermally, this external skin preparation has excellent effects on wound healing, prevention of rough skin, improvement of rough skin, and prevention of aging, and has led to the completion of the present invention.

[Means for Solving the Problems] That is, the present invention is an external skin preparation characterized by containing a deproteinized thymus extract and one or more vitamins.

Hereinafter, the configuration of the present invention will be explained in detail.

The deproteinized thymus extract used in the present invention can be obtained, for example, by the following method.

-15°C after mincing or homogenizing the animal-derived thymus
After degreasing with ether and treating with ether, pH 6.8-7.9
After adding a buffer solution and further adding an organic acid having a pH of 3 or less for decomposition and extraction, protease is added to the residue to obtain a crude extract. This crude extract is separated into a protein-free part and a protein-containing part by dialysis, and the latter is concentrated or freeze-dried, then hydrolyzed with enzymes to the bebutide fraction, residual protein is removed by acid/ethanol precipitation, and It is obtained by combining with the protein-removed portion of the product and concentrating in vacuo to a desired dry residue content, adjusting the pH to around 7, and then sterile filtration.

The amount of deproteinized thymus extract used in the present invention is 0.0001 to 5.0% by weight as a dry solid content in the total amount of the skin external preparation.

In the present invention, in addition to the above deproteinized thymus extract, one or more vitamins are blended.

The vitamins used in the present invention include vitamin A such as vitamin oil, redinol, and retinol acetate, 82 vitamins such as riboflavin, riboflavin butyrate, and flavin adenine dinucleotide, and pyridoxine hydrochloride and pyridoxine dioctanoate. 86 vitamins, L-ascorbic acid, L-ascorbic acid cibalmitic acid ester, vitamin C such as L-ascorbic acid-2-sodium sulfate, calcium pantothenate, D-bantothenyl alcohol, bantothenyl ethyl ether, acederbant Pantothenic acids such as thenyl ethyl ether, vitamin Ds such as ergocalciferol and cholecalciferol, nicotinic acids such as nicotinic acid, nicotinamide, and benzyl nicotinate, α-tocopherol, tocopherol acetate, nicotine MDL-α-tocopherol, Succinic acid D
Vitamin E such as L-α-tocopherol, vitamin P
1 biotin, etc.

The amount of vitamins added is 0゜000 of the total amount of the skin external preparation.
It is 1 to 5.0% by weight.

If the amount of deproteinized thymus extract or one or more types of vitamin A is less than 0.0001% by weight, the effect tends to be poor, and conversely, if the amount is more than 5.0% by weight, it will be effective. We cannot hope for a large increase in .

In addition to the above-mentioned essential ingredients, the external skin preparation of the present invention contains other ingredients commonly used in external skin preparations such as cosmetics and pharmaceuticals, such as avocado oil, palm oil, peanut oil, beef tallow, rice bran oil, jojoba oil, and evening primrose. Oil, carnauba wax, lanolin, liquid paraffin, squalane, isostearyl valmitate, isostearyl alcohol, glycerin tri-2-ethylhexanoate, glycerin, sorbitol, polyethylene glycol, batyl alcohol, collagen, hyaluronic acid, chondroitin sulfate, Humectants such as dextran sodium sulfate, amyl valadimethylaminobenzoate, 2-hydroxy-4-methoxybenzophenone-5-sulfone MNa.

UV absorbers such as urocanic acid, ethyl diisobrobyl cinnamate, antioxidant layers such as sodium erythorbate and parahydroxyanisole, sodium stearyl sulfate, jetanolamine cetyl sulfate, cetyl trimethylammonium saccharin, polyethylene glycol isostearate, arachidic acid Surfactants such as glyceryl and diglycerine diisostearate, preservatives such as ethylparaben and butylparaben, glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, salicylic acid derivatives, anti-inflammatory agents such as hinokitiol, zinc oxide, allantoin, placenta extract,
Glutathione, whitening agents such as saxifrage extract, sagebrush, orensis, chicon, peony, japonica, birch,
Sage, loquat, carrot, aloe, mallow, iris, grape, Japanese lily, loofah, lily, saffron, nebula, perforatum, rosemary, garlic, etc., royal billy, photosensitizer, cholesterol word Conductors, various raccoon amino acids, activators such as spleen extract and larval blood extract, blood circulation promoters such as γ-oryzanol, antiseborrheic agents such as sulfur and thianthol, carboxyvinyl polymer, carboxymethylcellulose, carboxyhuman - Thickeners such as roxyprovir cellulose, fragrances, water, alcohol, coloring agents such as titanium yellow, casamine, safflower red, resin powders such as polyethylene and nylon, etc. can be appropriately blended as necessary.

Further, the dosage form of the skin external preparation of the present invention is arbitrary, and may be, for example, a solubilized system such as a lotion, an emulsified system such as a milky lotion or a cream, or a dosage form such as an ointment or a dispersion.

[Effects of the Invention (Skin improvement effect test on human skin) Skin roughness improvement effect test on human body panel using the lotion obtained in Example 1 and blank lotion (not containing deproteinized thymus extract and tocopherol acetate) I did it.

In other words, the topic is to create a skin replica of the skin surface morphology of a healthy female person (facial) using the replica method using Millisne resin. Observe H using jlifm (17x magnification). Using 25 people (rough skin panel) who were judged to have a skin roughness rating of 1.2 based on the condition of the skin pattern and the llJm condition of the stratum corneum based on the criteria shown in Table 2, the test sample obtained in Example 1 was applied to the left and right half of the face. A blank lotion and a blank lotion were applied once a day for two weeks.

Two weeks later, the skin condition was observed again using the replica method described above and evaluated according to the criteria in Table 1.

(Margin below) Table-1 The results are shown in Table-2.

Table 2 From the results, it was found that the facial area treated with the lotion containing deproteinized thymus extract and tocopherol acetate had a remarkable effect on improving rough skin compared to the facial area treated with the blank lotion.

[Example] Next, the present invention will be explained in detail with reference to Examples. still,
The present invention is not limited thereby. The blending amount is in weight%.

Example 1 Lotion (1) Deproteinized thymus extract 0.05 (2) Tocopherol acetate 0.01 (3) Glycerin 4.0 (4) 1.3-butylene glycol 4.0 (5) Ethanol 7
．． 0 (6) Polyoxyethylene oleyl alcohol 0.5 (7) Methylparaben 0.05 (8) Citric acid 0.05 (9) Sodium citrate 0.1 (10) Fragrance
0.05 (11) Purified water
Residue (manufacturing method) Citric acid, sodium citrate, glycerin, purified water, 1.
3-Butylene glycol, dissolve the deproteinized thymus extract. Separately, polyoxyethylene oleyl alcohol, tocopherol acetate, fragrance, and methylparaben were dissolved in ethanol, and this was added to the above-mentioned purified aqueous solution to solubilize and filtered to obtain a lotion.

(Production method of protein-depleted thymus extract) Frozen calf thymus was cut into pieces, and acetone 501 was added to the
Leave it for an hour, add acetone 251 and leave it for 72 hours.
Remove fat-soluble parts. After separating the acetone layer, 15 L of ethyl ether and 5 to 10 liters of purified water were added to the residue, and -
After standing at 15°C to -25°C for 100 hours or more, centrifugation is performed. Add a buffer solution to the residue and perform separation and extraction twice in neutral conditions (a, b).
Add a solution containing 0.001% to 0.01% zinc and extract twice at pH 3 or lower (c, d).

Protease is added to the residue and reacted for 48 hours or more, and aldonic acid is further added to remove protein. Furthermore, the extract (e) separated for 1 to 2 hours and the previously obtained extract a''-
Combine d and add paraben to adjust pf (7.0:A), and then perform dialysis. After that, sterile filter and store at 4°C (f). Protein-containing parts are hydrolyzed with freeze-dried enzymes. After deproteinization and centrifugation, the resulting deproteinized extract g is concentrated with the previously deproteinized extract f, a preservative is added, and after pH adjustment, the mixture is concentrated until it contains dry solids of about 10z. Obtained by concentrating.

Example 2 Cream (1) Cetostearyl alcohol 3.5 (2) Squalane 40.0 (3) Beeswax 3.0 (4) Reduced lanolin
5.0(5) Ethylparaben
0.3 (6) Polyoxyethylene (20) Sorbitan monopalmitate ester 2.0 (7) Stearic acid monoglyceride 2.0 (8) Deproteinized thymus extract 0.5 Extract described in Example 1 (9) Retinol acetate 2.0 (10)
Liver extract 0.01 (11) Evening primrose oil 0.05 (12) Flavoring
0.03(13) 1.3-butylene glycol 5.0(14) Glycerin
5.0 (15) Purified water
Residue (manufacturing method) (1) (2) (3) (4) (5) (6) (
7), (11), and (12) were dissolved by heating and heated to 75°C. (8), (9), (10), (13), (14),
Add to 15) while stirring. After processing with a homomixer to make the emulsified particles fine, it is rapidly cooled while stirring, and the cream is added once again.

Example 3 Emulsion (1) Deproteinized thymus extract 0.01 Extract described in Example 1 (2) L-ascorbic acid-2-sulfate Na O,0
05(3) Stearic acid 1.5(
4) Seedyl alcohol 0.5 (5) Beeswax 2.0 (6) Polyoxyethylene (10) Monooleic acid ester 1.0 (7) Quince seed extract (5% aqueous solution) 20.0 (8) Hyaluron Acid Ha O,1(9
) Placenta extract 0.05 (10) Propylene glycol 5.0 (11) Ethanol 3.0 (12) Ethylparaben 0.3 (13) Fragrance 11
0.03 (14) Purified water
Residue (manufacturing method) Add fragrance to ethanol and dissolve (alcohol phase).

Propylene glycol and hyaluronic acid Ha are added to purified water, dissolved by heating, and kept at 70°C (aqueous phase). The other ingredients except the Kuisseed extract are mixed, heated and dissolved and kept at 70°C (oil phase). Pre-emulsify by adding oil to the aqueous phase, and homogeneously emulsify using a homomixer. Add the alcohol phase and quince seed extract while stirring. Thereafter, the mixture was cooled to 30° C. while stirring to obtain a milky lotion.

Example 4 Pack (1) Deproteinized thymus extract 0.05 Example 1
Extract as described (2) Cholecalciferol 0.01 (3) Polyvinyl alcohol 15.0 (4) Polyethylene glycol 3.0 (5) Propylene glycol
7.0 (6) Ethanol 10.
0(7) Methylparaben 0.05(8)
Fragrance 1EI 0.05 (9) Purified water Remaining (manufacturing method) Add polyethylene glycol, propylene glycol, methyl paraben, deproteinized thymus extract, and cholecalcifera to purified water and dissolve with stirring.

Next, add polyvinyl alcohol and stir silently.
Add ethanol dissolved in I! A pack was obtained by stirring and dissolving.

Example 6 Ointment (1) Deproteinized thymus extract 0.5 Example 1 Extract of the war (2) Tocopherol acetate 1.0 (3) Resonol palmitate 0.5 (4) Stearyl alcohol 18.0 (5 ) Mokuro
20.0 (6) Polyoxyethylene (10) Monooleate 0.25 (7) Glycerin monostearate 0.3 (8) Vaseline 40.0 (9) Purified water Remaining (manufacturing method) Purified water 70 Keep the temperature at 70°C (aqueous phase) and mix and dissolve the following ingredients at 70°C (oil I■). The oil phase was added to the water phase, uniformly emulsified using a homomixer, and then cooled to obtain an ointment.

Patent applicant: Shiseido Co., Ltd.

Claims (1)

[Claims] 1. A skin preparation for external use, characterized by containing a deproteinized thymus extract and one or more vitamins.