CN107348294A - A kind of bird's nest collagen solid beverage and preparation method thereof - Google Patents

A kind of bird's nest collagen solid beverage and preparation method thereof Download PDF

Info

Publication number
CN107348294A
CN107348294A CN201710553733.1A CN201710553733A CN107348294A CN 107348294 A CN107348294 A CN 107348294A CN 201710553733 A CN201710553733 A CN 201710553733A CN 107348294 A CN107348294 A CN 107348294A
Authority
CN
China
Prior art keywords
bird
nest
collagen
solid beverage
collagen solid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710553733.1A
Other languages
Chinese (zh)
Other versions
CN107348294B (en
Inventor
程彦
邓颖妍
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Funuo Nutrition Technology Co Ltd
Original Assignee
Guangzhou Funuo Nutrition Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Funuo Nutrition Technology Co Ltd filed Critical Guangzhou Funuo Nutrition Technology Co Ltd
Priority to CN201710553733.1A priority Critical patent/CN107348294B/en
Publication of CN107348294A publication Critical patent/CN107348294A/en
Application granted granted Critical
Publication of CN107348294B publication Critical patent/CN107348294B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385—Concentrates of non-alcoholic beverages
    • A23L2/39—Dry compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/62—Clouding agents; Agents to improve the cloud-stability
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/238—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin from seeds, e.g. locust bean gum or guar gum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dispersion Chemistry (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention belongs to food technology field, and in particular to a kind of bird's nest collagen solid beverage and preparation method thereof.Bird's nest collagen solid beverage provided by the invention includes following components and its percentage by weight：Bird's nest powder 5 30%, collagen 35 45%, water-soluble dietary fiber 0.5 1.5%, sweetener 15 28% and guar gum 18 22%.Bird's nest collagen solid beverage provided by the invention has the function that toxin-expelling and face nourishing, lipid-lowering weight-reducing, while can also increase sialic acid content in nursing period milk, promotes baby's early stage intellectual development.

Description

A kind of bird's nest collagen solid beverage and preparation method thereof
Technical field
The invention belongs to food technology field, and in particular to a kind of bird's nest collagen solid beverage and preparation method thereof.
Background technology
After women childbirth, it can often be perplexed by various problems, wherein the most prominent is milk abundance, idiotrophic mistake Caused by surplus, sleep insufficiency skin deterioration, striae of pregnancy, stature change, constipation etc..And in during lactation, women also can lattice Outer concern diet, generally using normal diet as the supplement of nutrition, such as bird's nest, chicken soup, pig's feet, ginger etc., wherein bird's nest is even more The fine work being described as in tonic.The main component of bird's nest is water soluble protein, carbohydrate, trace element and amino acid Deng why bird's nest has high nutritive value, is largely because bird's nest and is rich in sialic acid.Sialic acid is one group of god Derivative through propylhomoserin, it is the necessary nutrient of human brain cognitive development.Saliva in studies have shown that nursing period mother milk Acid has obvious facilitation to baby's early stage intellectual development.However, the edible preparation of bird's nest takes, there is inconvenience.
Collagen is a kind of important foodstuffs of women beauty, has the function that moisturizing, crease-resistant, can supplement needed for skin Nutrition, strengthen Collage Activitv in skin, skin care, anti-aging, beauty, wrinkle-chasing and other effects.At present, also have on the market not Few food using collagen as primary raw material, it is mainly used in women beauty, also there is small part, increase on the basis of collagen Bird's nest powder is added, to increase its cosmetic result.But sialic acid content and indefinite and right in the cosmetics of the powder containing bird's nest The influence of sialic acid is smaller in nursing period milk, is unfavorable for the development of baby.
Chinese patent application CN105795301A discloses a kind of solid beverage with Elmination of toxicant and fat-lowering effect and its preparation Method, the solid beverage, include the composition of following weight fraction：Collagen 5-20 parts, dietary fiber 10-80 parts, the root of kudzu vine Powder 2-10 parts, xylitol 5-20 parts.The invention solid beverage has the function that toxin-expelling and face nourishing, but long-term use can be to nursing period mother Sialic acid content in mother's emulsion has negatively influencing.
Chinese patent application CN106579375A discloses a kind of bird's nest collagen peptide composite powder, by following parts by weight Raw material composition, Isin glue collagen peptide 15-25 parts, bird's nest 3-7 parts, apple powder 25-35 parts, lemon powder 8-12 parts, blueberry powder 12- 16 parts, water-soluble dietary fiber 15-16 parts, vitamin C 1-3 parts, vitamin E 1-3 parts, vitaminB10 .01-0.05 parts, dimension Raw plain B20.01-0.05 parts, vitamin B6 0.01-0.05 parts, vitamin B12 0.01-0.03 parts, beta carotene 1-3 parts and cigarette Acid amides and folic acid 0.1-0.3 parts.The invention bird's nest collagen peptide composite powder long-term use can with coordinating intestines and stomach, fat-reducing, but its Mouthfeel is undesirable, and the influence after taking to sialic acid in milk is smaller.
The content of the invention
In order to solve problems of the prior art, it is an object of the invention to provide a kind of bird's nest collagen solids Beverage and preparation method thereof.Bird's nest collagen solid beverage provided by the invention has the function that toxin-expelling and face nourishing, lipid-lowering weight-reducing, Sialic acid content in nursing period milk can be increased simultaneously, promote baby's early stage intellectual development.
The invention provides a kind of bird's nest collagen solid beverage, including following components and its percentage by weight：
Bird's nest powder 5-30%, collagen 35-45%, water-soluble dietary fiber 0.5-1.5%, sweetener 15-28% and Guar gum 18-22%.Water-soluble dietary fiber is purchased from Shaanxi Sen Fu natural products Co., Ltd.
Further, described bird's nest collagen solid beverage is made up of following components and its percentage by weight：
Bird's nest powder 17.5%, collagen 40%, water-soluble dietary fiber 1%, sweetener 21.5% and guar gum 20%.
Further, the preparation method of the collagen is：
1) fish-skin 2g is taken, adds 100-150mL hydrogen peroxide-sodium hydroxide solution, decolourize 24h at 20 DEG C, and distilled water is clear Wash, airing, obtain degreasing fish-skin；
2) degreasing fish-skin 2g and 0.1-0.3g enzyme obtained by taking step 1), is added in 5-15mL 0.4M acetums, 10-20 DEG C of extraction 48-64h, filters, concentrates, spray drying, produce.
Further, in the step 1) hydrogen peroxide-sodium hydroxide solution by hydrogen peroxide, sodium hydroxide and water in mass ratio (0.5-1.5)：(0.04-0.1)：100 compositions.
Further, in the step 2) enzyme by pepsin and papain in mass ratio 3:1 composition.
Further, one or more of the described sweetener in xylitol, D-sorbite, antierythrite.
Meanwhile present invention provides a kind of preparation method of bird's nest collagen solid beverage, comprise the following steps：
S1 mixes bird's nest powder, collagen, water-soluble dietary fiber, homogeneous, obtains mixture A；
S2 grinds sweetener, guar gum, crosses 60-80 mesh, is well mixed, obtains mixture B；
Mixture A and mixture B are sufficiently mixed by S3, are sterilized, and are dried, are produced.
Collagen is the main component of skin, forms a fine and closely woven elasticated net in skin, pins moisture, supports skin Skin, there is the effect such as moisturizing, dewrinkling spot-eliminating, whitening.The main component of bird's nest is water soluble protein, carbohydrate, micro Why element and amino acid etc., bird's nest have high nutritive value, are to be rich in sialic acid, and sialic acid can improve human body note Recall power and IQ, improve the effect such as body immunity, anti-dementia.The diet of nursing period directly affects nursing period mother breast The content of sialic acid in juice, inventor, which studies, to be found, the intake of bird's nest and collagen can change containing for sialic acid in milk Amount, but water-soluble dietary fiber has when promoting intestines peristalsis indirectly reduces its content, is studied by the later stage, inventor obtains Technical scheme is arrived, the final content for causing sialic acid in nursing period mother's milk significantly improves.
Compared with prior art, bird's nest collagen solid beverage provided by the invention has following technical advantage：
(1) bird's nest collagen solid beverage provided by the invention can recover elasticity of skin with Glycerin, anti-oxidant, Slowing down skin aging, wrinkle is reduced, color spot is eliminated, so as to reach the effect of beautifying face and moistering lotion.
(2) sialic acid content reaches 30-50mg in bird's nest collagen solid beverage provided by the invention, and eats and be easy to Absorb, the content of sialic acid in nursing period mother's milk, more conducively baby's early stage intellectual development can be improved.
(3) bird's nest collagen solid beverage provided by the invention can promote the wriggling of enteron aisle, prevent constipation, effectively Vivotoxin is excluded, the effect of playing toxin expelling, lipid-loweringing, reduce weight.
(4) bird's nest collagen solid beverage formula provided by the invention is simple, in good taste, no fishlike smell, preserves stabilization, Carry, be convenient to take.
Embodiment
Below by specific embodiment the present invention will be further explained explanation, these embodiments are only used for the mesh of illustration , it is not intended to limit protection scope of the present invention.
A kind of bird's nest collagen solid beverage of embodiment 1
The bird's nest collagen solid beverage is made up of following components and its percentage by weight：
Bird's nest powder 5%, collagen 45%, water-soluble dietary fiber 0.5%, sweetener 28% and guar gum 21.5%.
The preparation method of the collagen is：
1) fish-skin 2g is taken, adds 125mL hydrogen peroxide-sodium hydroxide solution, decolourize 24h at 20 DEG C, distilled water cleaning, cool It is dry, obtain degreasing fish-skin；
2) degreasing fish-skin 2g and 0.2g enzyme obtained by taking step 1), is added in 10mL 0.4M acetums, in 20 DEG C of extractions 56h, filter, concentrate, spray drying, produce.
Hydrogen peroxide-sodium hydroxide solution is by hydrogen peroxide, sodium hydroxide and water in mass ratio 1 in the step 1)：0.07： 100 compositions.
Enzyme is by pepsin and papain in mass ratio 3 in the step 2):1 composition.
Preparation method：
S1 mixes bird's nest powder, collagen, water-soluble dietary fiber, homogeneous, obtains mixture A；
S2 grinds sweetener, guar gum, crosses 70 mesh, is well mixed, obtains mixture B；
Mixture A and mixture B are sufficiently mixed by S3, are sterilized, and are dried, are produced.
A kind of bird's nest collagen solid beverage of embodiment 2
The bird's nest collagen solid beverage is made up of following components and its percentage by weight：
Bird's nest powder 17.5%, collagen 40%, water-soluble dietary fiber 1%, sweetener 21.5% and guar gum 20%.
The preparation method of the collagen is similar to Example 1.
Preparation method is similar to Example 1.
A kind of bird's nest collagen solid beverage of embodiment 3
The bird's nest collagen solid beverage is made up of following components and its percentage by weight：
Bird's nest powder 30%, collagen 35%, water-soluble dietary fiber 1.5%, sweetener 15% and guar gum 18.5%.
The preparation method of the collagen is similar to Example 1.
Preparation method is similar to Example 1.
A kind of bird's nest collagen solid beverage of comparative example 1
The bird's nest collagen solid beverage is made up of following components and its percentage by weight：
Bird's nest powder 17.5%, collagen 40%, water-soluble dietary fiber 1%, sweetener 21.5% and guar gum 20%.
Hydrogen peroxide-sodium hydroxide solution in the collagen in step 1) replaces with sodium chloride-sodium hydroxide Solution, remaining step are same as Example 1.
Preparation method is similar to Example 1.
Difference with embodiment 2 is, hydrogen peroxide-sodium hydroxide solution in the preparation method of collagen is replaced with into chlorine Change sodium-sodium hydroxide solution.
A kind of bird's nest collagen solid beverage of comparative example 2
The bird's nest collagen solid beverage is made up of following components and its percentage by weight：
Bird's nest powder 17.5%, collagen 40%, water-soluble dietary fiber 1%, sweetener 21.5% and guar gum 20%.
Enzyme in the collagen in step 2) is pepsin, and remaining step is same as Example 1.
Preparation method is similar to Example 1.
Difference with embodiment 2 is that enzyme is different in the preparation method of collagen.
A kind of bird's nest collagen solid beverage of comparative example 3
The bird's nest collagen solid beverage is made up of following components and its percentage by weight：
Bird's nest powder 17.5%, collagen 40%, water-soluble dietary fiber 1%, sweetener 21.5% and guar gum 20%.
The preparation method of the collagen is similar to Example 1.
Preparation method is similar to Example 1.
Difference with embodiment 2 is, guar gum is replaced with into maltodextrin.
Test example one, toxin expelling experiment
1. test material：Bird's nest collagen solid beverage prepared by embodiment 1-3 and comparative example 3.
2. subjects：Kunming kind male SPF levels mouse 50,6-8 week old, body weight 18-22g.
3. test method：
Mouse is randomly divided into 5 groups, every group 10, respectively 3 groups of control group, embodiment 1-3 groups and comparative example.On an empty stomach, The bird's nest collagen solid beverage (0.03g/mL) prepared with 0.5mL distilled water, embodiment 1-3 and comparative example 3 is filled respectively, Continuously gavage 7 days, after last gives sample each group mouse fasting can't help water 16h, give prepared Chinese ink, animal subject put to death after 20min, Abdominal cavity separation mesenterium is opened, clip upper end is placed on pallet from pylorus, lower extremely ileocecal intestinal tube, small intestine is pulled into straight line Measurement.Length of intestine is " total small intestinal length ", is " prepared Chinese ink propulsion length " from pylorus to prepared Chinese ink forward position.Calculation formula is：Prepared Chinese ink Propulsion rate %=(prepared Chinese ink promotes length/total small intestinal length) × 100%, and weigh stool in mice weight.
4. result of the test：Prepared Chinese ink promotes and stool in mice weight the results are shown in Table 1.
The prepared Chinese ink of table 1 promotes and stool in mice weight result
Group Ink progradation/% Excrement amount/mg
Control group 40.15±6.10 35.56±4.35
1 group of embodiment 60.73±10.44*# 49.53±6.86*#
2 groups of embodiment 68.43±9.85**# 53.85±7.04**#
3 groups of embodiment 64.59±8.63**# 51.75±5.63**#
3 groups of comparative example 50.12±7.21* 42.32±5.62*
Note：* with control group than P ＜ 0.05, * * are with control group than P ＜ 0.01；# is with 3 groups of comparative example than P ＜ 0.05.
As shown in Table 1, the bird's nest collagen solid beverage that prepared by the present invention has significant impetus to mouse small intestine, Illustrate that bird's nest collagen solid beverage of the present invention can substantially increase intestines peristalsis, promote the effect of defecation toxin expelling.With contrast 3 groups of example is compared, and bird's nest collagen solid beverage prepared by 1-3 of the embodiment of the present invention has aobvious to the impetus of mouse small intestine Work property, it is preferable to illustrate that the present invention uses the solid beverage of guar gum preparation to reconstitute rear effect of ejecting poison.
Test example two, lipid-loweringing experiment
1. test material：Bird's nest collagen solid beverage prepared by embodiment 1-3, comparative example 1 and comparative example 3.
2. subjects：SPF levels rat 50, male and female half and half, body weight 60-90g.
3. test method：
Rat is randomly divided into 5 groups, every group 10, respectively 3 groups of control group, embodiment 1-3 groups and comparative example.On an empty stomach, The bird's nest collagen solid beverage (0.03g/mL) prepared with 1mL distilled water, embodiment 1-3 and comparative example 3 is filled respectively, often It once, totally 30 days.Using automatic clinical chemistry analyzer, T-CHOL, triglyceride content in blood are determined.
4. result of the test：T-CHOL, content of triglyceride the results are shown in Table 2 in blood.
T-CHOL, triglyceride content results in the blood of table 2
Known by table 2, compared with control group, bird's nest collagen solid beverage prepared by the present invention is consolidated to total courage of rat Alcohol, content of triglyceride have declined, it is seen that the bird's nest collagen solid beverage plays the role of certain reducing blood lipid.It is although right Ratio 3 groups of T-CHOLs, content of triglyceride have declined, but its effect illustrates that the present invention matches somebody with somebody but not as good as embodiment 1-3 groups 5 more rationally, more preferable to the effect of norcholesterol, triglycerides.
Test example three, sialic acid content test
(1) test material：Bird's nest collagen solid beverage prepared by embodiment 1-3 and comparative example 1-3.
(2) subjects：The puerpera of spontaneous labor, 140 people, age 25-29 year, the 1st tire.
(3) test method：
1. puerpera is divided into 7 groups, every group of 20 people, control group, embodiment 1-3 and comparative example 1-3 groups are denoted as.
2. each group puerpera's early morning in postpartum empty stomach difference drinkable water, bird's nest collagen egg prepared by embodiment 1-3 and comparative example 1-3 White solid beverage, it is used continuously one month, collects milk, and determine sialic acid content in milk.
3. sialic acid content determines in milk
(1) measure dissociated with oligosaccharide bound sialic acid content
500 μ L milk accurately are measured in centrifuge tube, add the solution of trichloroacetic acid protein precipitation of isometric 10%, are mixed Ice bath 10min after even, then centrifuge 30min, supernatant is moved into new centrifuge tube, added in precipitation 500 μ L it is cold 5% three Chloroethene acid elution, merge two into the supernatant of the 1st time in 4 DEG C, 3000r/min centrifugation 30min, Aspirate supernatant after mixing Secondary supernatant.The supernatant of merging adds isometric 80 DEG C of hydrolysis 30min of 0.1mol/L trifluoroacetic acids, cools down, passes through after taking-up 0.22 μm of membrane filtration, the accurate μ L of solution 90 drawn after filtering, 10 μ L DMB, 50 DEG C of lucifuges are added to derive 150min, after cooling Liquid spectrum analysis is carried out, this is sialic acid sum dissociating in breast milk and being combined with oligosaccharide.
(2) measure of protein binding sialic acid content
2mL (0.05mol/L) sulfuric acid is added in precipitation after step 1) processing, 80 DEG C of hydrolysis 120min, is cooled down after taking-up, Through 0.22 μm of membrane filtration, the accurate μ L of solution 90 drawn after filtering, 10 μ L DMB, 50 DEG C of lucifuges are added to derive 150min, cooling Analyzed afterwards by chromatographic condition.This be breast milk in protein bound sialic acid content.
In breast milk Total silicic acid content for it is free, being combined with oligosaccharide and with protein bound sialic acid content it With.
(3) chromatographic test strip part
Chromatographic column：LiChrosorb RP-18 posts (250mm × 4mm, 5 μm), 30 DEG C of column temperature；Fluorescence detector excitation wavelength 373nm, launch wavelength 448nm；Mobile phase is methanol-acetonitrile-ultra-pure water (7:8:85)；Flow velocity is 0.9mL/min；Sampling volume 10μL.DMB derives liquid：8mmol/L DMB, 1.5mol/L glacial acetic acid, 0.25mol/L sodium thiosulfate, 0.25mol/L sulfurous acid Sodium, 0.8mmol/L 2 mercapto ethanols.Derivatization conditions：10 μ L DMB are added in 90 μ L samples or standard items and derive liquid, 50 DEG C are kept away Light derives 150min, and liquid spectrum analysis is carried out after being cooled to room temperature.
4. the measure of sialic acid content in sample
Each 3g of bird's nest collagen solid beverage prepared by Example 1-3 and comparative example 1-3, add in 100mL water, stir Mix uniformly, as sample solution.The assay method of sialic acid content determines with sialic acid content in milk in sample.
(4) result of the test：Sialic acid content measurement result in milk is shown in Table 3, the measure knot of sialic acid content in sample Fruit is shown in Table 4.
Sialic acid content (mg/L) in the milk of table 3
Sialic acid content (mg/L) in the sample of table 4
As shown in Table 3, after the bird's nest collagen solid beverage that prepared by the edible present invention, the sialic acid in puerpera's milk contains The obvious increase of amount.Compared with comparative example 1-3, bird's nest collagen solid beverage prepared by 1-3 of the embodiment of the present invention to puerpera and The effect of baby is more preferable, is more beneficial for the development of baby, and the wherein embodiment of the present invention 2 is most preferred embodiment, is produced after edible January The content of sialic acid adds 17% in woman's milk.
As shown in Table 4,30-50mg salivas are contained in product of the present invention in every bag of (3g) bird's nest collagen solid beverage Acid, hence it is evident that better than comparative example 1-3 groups.
Test example four, sensory testing
1. test material：Bird's nest collagen solid beverage prepared by embodiment 1-3 and comparative example 1-3.
2. subjects：The puerpera of spontaneous labor, 120 people, age 25-29 year, the 1st tire.
3. test method：
Subject is randomly divided into 6 groups, every group of 20 people, is denoted as embodiment 1-3 and comparative example 1-3 groups.Each group is eaten respectively Bird's nest collagen solid beverage (0.03g/mL) prepared by embodiment 1-3 and comparative example 1-3, daily morning is edible on an empty stomach, To its mouthfeel and rear ocular estimate is reconstituted after January, marking evaluation, standards of grading are carried out to cosmetic result：Using ten point system, 10 Point best, 0 point worst, and fraction more high effect is better.
4 result of the tests：Analyses Methods for Sensory Evaluation Results is shown in Table 5.
The Analyses Methods for Sensory Evaluation Results of table 5
As shown in Table 5, the bird's nest collagen solid beverage for preparing of the present invention is in good taste without fishlike smell, reconstitutes rear solution It is bright, skin high resilience can be made after long-term consumption, and with moisturizing, crease-resistant, skin whitening effect.Compared with comparative example 1-3, this The character and consumption of bird's nest collagen solid beverage prepared by inventive embodiments 1-3 are preferable, illustrate inventive formulation it is reasonable, Each component interacts, so as to play beautifying face and moistering lotion, the effect of Elmination of toxicant and fat-lowering, and good in taste, no fishlike smell.

Claims (7)

1. a kind of bird's nest collagen solid beverage, it is characterised in that including following components and its percentage by weight：Bird's nest powder 5- 30%th, collagen 35-45%, water-soluble dietary fiber 0.5-1.5%, sweetener 15-28% and guar gum 18-22%.
2. bird's nest collagen solid beverage as claimed in claim 1, it is characterised in that by following components and its weight percent Than composition：
Bird's nest powder 17.5%, collagen 40%, water-soluble dietary fiber 1%, sweetener 21.5% and guar gum 20%.
3. bird's nest collagen solid beverage as claimed in claim 1 or 2, it is characterised in that the preparation of the collagen Method is：
1) fish-skin 2g is taken, adds 100-150mL hydrogen peroxide-sodium hydroxide solution, decolourize 24h at 20 DEG C, distilled water cleaning, cool It is dry, obtain degreasing fish-skin；
2) degreasing fish-skin 2g and 0.1-0.3g enzyme obtained by taking step 1), is added in 5-15mL 0.4M acetums, in 10-20 DEG C extraction 48-64h, filter, concentrate, spray drying, produce.
4. bird's nest collagen solid beverage as claimed in claim 3, it is characterised in that hydrogen peroxide-hydrogen in the step 1) Sodium hydroxide solution is by hydrogen peroxide, sodium hydroxide and water (0.5-1.5) in mass ratio：(0.04-0.1)：100 compositions.
5. bird's nest collagen solid beverage as claimed in claim 3, it is characterised in that enzyme is by stomach cardia in the step 2) Enzyme and papain in mass ratio 3:1 composition.
6. bird's nest collagen solid beverage as claimed in claim 1 or 2, it is characterised in that described sweetener is selected from wood One or more in sugar alcohol, D-sorbite, antierythrite.
7. the preparation method of the bird's nest collagen solid beverage as described in claim 1-6 is any, it is characterised in that including with Lower step：
S1 mixes bird's nest powder, collagen, water-soluble dietary fiber, homogeneous, obtains mixture A；
S2 grinds sweetener, guar gum, crosses 60-80 mesh, is well mixed, obtains mixture B；
Mixture A and mixture B are sufficiently mixed by S3, are sterilized, and are dried, are produced.
CN201710553733.1A 2017-07-08 2017-07-08 Cubilose collagen solid beverage and preparation method thereof Active CN107348294B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710553733.1A CN107348294B (en) 2017-07-08 2017-07-08 Cubilose collagen solid beverage and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710553733.1A CN107348294B (en) 2017-07-08 2017-07-08 Cubilose collagen solid beverage and preparation method thereof

Publications (2)

Publication Number Publication Date
CN107348294A true CN107348294A (en) 2017-11-17
CN107348294B CN107348294B (en) 2020-09-04

Family

ID=60291857

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710553733.1A Active CN107348294B (en) 2017-07-08 2017-07-08 Cubilose collagen solid beverage and preparation method thereof

Country Status (1)

Country Link
CN (1) CN107348294B (en)

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107969691A (en) * 2017-11-28 2018-05-01 北京同仁堂健康药业股份有限公司 A kind of composition that can effectively improve moisture of skin and preparation method thereof
CN108902940A (en) * 2018-06-28 2018-11-30 燕生（福建）生物工程有限公司 A kind of instant bird's nest collagen Gly-His-Lys and preparation method thereof
CN108902612A (en) * 2018-08-15 2018-11-30 燕之初健康美（厦门）食品有限公司 A kind of uniform caramel bird nest drink of nutrition and preparation method thereof and application method
CN109329690A (en) * 2018-11-22 2019-02-15 兴化市东奥食品有限公司 A kind of novel nourishing type solid beverage
CN109602021A (en) * 2018-06-28 2019-04-12 丽睿客信息科技（北京）有限公司 Nutraceutical containing sialic acid
CN110679808A (en) * 2019-01-22 2020-01-14 余庆东 Colored jam and cubilose beverage and preparation method thereof
CN111034885A (en) * 2019-12-31 2020-04-21 本草元素生物科技(厦门)有限公司 Bird's nest elastin peptide plant beverage and preparation method thereof
CN111165709A (en) * 2020-02-27 2020-05-19 湖州中科锦肽生物科技有限公司 Solid beverage with blood sugar control function and preparation method thereof
CN111955632A (en) * 2020-08-19 2020-11-20 韩绪 Sialic acid-containing beverage and preparation method thereof
CN113243522A (en) * 2021-05-07 2021-08-13 海南大洲金丝燕产业集团有限公司 High-quality fresh stewed cubilose and preparation method thereof
CN113424964A (en) * 2021-06-17 2021-09-24 汤臣倍健股份有限公司 Composition and its use in preparing health food or food for regulating human milk nutrition and immunity components
CN113607830A (en) * 2021-06-15 2021-11-05 嘉必优生物技术（武汉）股份有限公司 Method for detecting sialic acid content in food
CN114158738A (en) * 2021-12-07 2022-03-11 喜园(广东)生物科技有限公司 Antioxidant beautifying cubilose collagen peptide composition and preparation method thereof
CN114711362A (en) * 2022-03-31 2022-07-08 湖北瑞邦生物科技有限公司 Antioxidant cubilose collagen peptide solid beverage and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003095961A (en) * 2001-09-27 2003-04-03 Combi Corp Skin beautifying promoter
CN102406214A (en) * 2011-11-23 2012-04-11 大连普瑞康生物技术有限公司 Solid beverage with skin beautifying function
CN103478811A (en) * 2013-09-11 2014-01-01 南京泽朗农业发展有限公司 Blueberry-blackberry mixed juice beverage and preparation method thereof
CN106360243A (en) * 2016-08-30 2017-02-01 黑龙江省玄鸟生物科技股份有限公司 White fungus composite solid beverage having anti-oxidation function and preparation method of white fungus composite solid beverage
CN106579375A (en) * 2016-11-30 2017-04-26 洛阳华以生物工程有限公司 Edible bird's nest collagen peptide composite powder and preparation technology thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003095961A (en) * 2001-09-27 2003-04-03 Combi Corp Skin beautifying promoter
CN102406214A (en) * 2011-11-23 2012-04-11 大连普瑞康生物技术有限公司 Solid beverage with skin beautifying function
CN103478811A (en) * 2013-09-11 2014-01-01 南京泽朗农业发展有限公司 Blueberry-blackberry mixed juice beverage and preparation method thereof
CN106360243A (en) * 2016-08-30 2017-02-01 黑龙江省玄鸟生物科技股份有限公司 White fungus composite solid beverage having anti-oxidation function and preparation method of white fungus composite solid beverage
CN106579375A (en) * 2016-11-30 2017-04-26 洛阳华以生物工程有限公司 Edible bird's nest collagen peptide composite powder and preparation technology thereof

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107969691A (en) * 2017-11-28 2018-05-01 北京同仁堂健康药业股份有限公司 A kind of composition that can effectively improve moisture of skin and preparation method thereof
CN108902940A (en) * 2018-06-28 2018-11-30 燕生（福建）生物工程有限公司 A kind of instant bird's nest collagen Gly-His-Lys and preparation method thereof
CN109602021A (en) * 2018-06-28 2019-04-12 丽睿客信息科技（北京）有限公司 Nutraceutical containing sialic acid
CN108902612B (en) * 2018-08-15 2021-07-16 燕之初健康美（厦门）食品有限公司 Caramel cubilose beverage with uniform nutrition and preparation method and use method thereof
CN108902612A (en) * 2018-08-15 2018-11-30 燕之初健康美（厦门）食品有限公司 A kind of uniform caramel bird nest drink of nutrition and preparation method thereof and application method
CN109329690A (en) * 2018-11-22 2019-02-15 兴化市东奥食品有限公司 A kind of novel nourishing type solid beverage
CN110679808A (en) * 2019-01-22 2020-01-14 余庆东 Colored jam and cubilose beverage and preparation method thereof
CN111034885A (en) * 2019-12-31 2020-04-21 本草元素生物科技(厦门)有限公司 Bird's nest elastin peptide plant beverage and preparation method thereof
CN111165709A (en) * 2020-02-27 2020-05-19 湖州中科锦肽生物科技有限公司 Solid beverage with blood sugar control function and preparation method thereof
CN111955632A (en) * 2020-08-19 2020-11-20 韩绪 Sialic acid-containing beverage and preparation method thereof
CN113243522A (en) * 2021-05-07 2021-08-13 海南大洲金丝燕产业集团有限公司 High-quality fresh stewed cubilose and preparation method thereof
CN113607830A (en) * 2021-06-15 2021-11-05 嘉必优生物技术（武汉）股份有限公司 Method for detecting sialic acid content in food
CN113607830B (en) * 2021-06-15 2023-08-08 嘉必优生物技术（武汉）股份有限公司 Method for detecting sialic acid content in food
CN113424964A (en) * 2021-06-17 2021-09-24 汤臣倍健股份有限公司 Composition and its use in preparing health food or food for regulating human milk nutrition and immunity components
CN114158738A (en) * 2021-12-07 2022-03-11 喜园(广东)生物科技有限公司 Antioxidant beautifying cubilose collagen peptide composition and preparation method thereof
CN114711362A (en) * 2022-03-31 2022-07-08 湖北瑞邦生物科技有限公司 Antioxidant cubilose collagen peptide solid beverage and preparation method thereof

Also Published As

Publication number Publication date
CN107348294B (en) 2020-09-04

Similar Documents

Publication Publication Date Title
CN107348294A (en) A kind of bird's nest collagen solid beverage and preparation method thereof
CN104322767B (en) A kind of nostoc milk tea, nostoc milk tea powder and preparation method
KR101272602B1 (en) Pomegranate extract containing a high content of ellagic acid, and a method for improving effect of pomegranate extract related to postmenopausal syndrome
CN110876258B (en) Beetroot juice for improving intestinal absorption rate, its preparation method and application
CN109350705A (en) A kind of composition and application with blood-enriching face-nourishing and strengthen immunity effect
CN105795301A (en) Solid beverage with functions of removing toxins and lowering lipid as well as preparation method of solid beverage
CN106722999A (en) A kind of collagen peptide preparation method, the low sugar collagen beauty treatment cake comprising it and preparation method
CN103108644B (en) Fat oxidation or energy metabolism enhancer
KR100699782B1 (en) Food composition for improving liver function comprising a Lonicera caerulea L. var. edulis extract
CN106306921A (en) Method for preparing chitosan oligosaccharide functional beverage with Maca and Cordyceps sinensis and functional beverage made thereby
CN106119044A (en) A kind of looks improving and the skin nourishing cider and preparation method thereof
JP2002068993A (en) Epidermal keratinizing cell propagation-accelerating agent, beautifying food and skin cosmetic
KR100773457B1 (en) Composition promoting hair growth and use thereof
CN103082169B (en) Composite collagenous fruit powder
CN109601894A (en) A kind of egg roll
CN105011047B (en) Children's morning refreshment and preparation method thereof
CN106509523A (en) Deer skin glue beverage
CN101632456A (en) Dwarf lilyturf drink and preparation method of nutrient solution thereof
CN109275898A (en) A kind of replenishing blood and nourishing liver cream and preparation method thereof
CN109757629A (en) A kind of compound yacon liquid meal composition and its preparation and application
CN104886584B (en) Adult's morning refreshment and preparation method thereof
CN104366511A (en) High-density reinforced protein oral liquid
CN109567158A (en) A kind of composition of anti-aging and anti-senile preparation including it
CN1692783A (en) Donkey-hide gelatin milk, and its prepn. method
CN107982513A (en) A kind of salt algae earthworm protein compound recipe health products and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of invention: A bird's nest collagen solid beverage and its preparation method

Effective date of registration: 20230331

Granted publication date: 20200904

Pledgee: China Construction Bank Co.,Ltd. Guangzhou Haizhu Branch

Pledgor: GUANGZHOU FUNUO NUTRITION TECHNOLOGY Co.,Ltd.

Registration number: Y2023980036930

PE01 Entry into force of the registration of the contract for pledge of patent right