CN104322767B - A kind of nostoc milk tea, nostoc milk tea powder and preparation method - Google Patents

A kind of nostoc milk tea, nostoc milk tea powder and preparation method Download PDF

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CN104322767B
CN104322767B CN201410603950.3A CN201410603950A CN104322767B CN 104322767 B CN104322767 B CN 104322767B CN 201410603950 A CN201410603950 A CN 201410603950A CN 104322767 B CN104322767 B CN 104322767B
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nostoc
powder
milk tea
milk
phycobniliprotein
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CN104322767A (en
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王卫
周丰
安彦君
易思富
熊斌
杨胜辉
王远忠
陈牧
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HUNAN YANDI BIOLOGICAL ENGINEERING Co Ltd
CHANGDE YANDI BIOLOGICAL TECHNOLOGY Co Ltd
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HUNAN YANDI BIOLOGICAL ENGINEERING Co Ltd
CHANGDE YANDI BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The present invention relates to field of food, particularly a kind of nostoc milk tea and nostoc milk tea powder and preparation method thereof.Nostoc milk tea of the present invention, comprise following component by weight: the tea extract of 20-30 part, the milk of 40-60 part, the nostoc phycobniliprotein crude extract of 10-20 part, the nostoc polysaccharide gel nutrient solution of 15-25 part, the flavor enhancement of 1-10 part, the sucrose fatty ester of 0.1-0.5 part, the citric acid of 0.1-0.4 part, the sweetener of 4-8 part, the honey of 5-8 part, 0.05-0.2 part thickener, 200 ~ 500 parts of water.Nostoc phycobniliprotein crude extract and nostoc polysaccharide gel nutrient solution join as natural colouring matter and stabilizing agent in milk tea by the present invention, not only make milk tea be in bright colors, and mellow in taste, the more important thing is to have unique medicinal health care function.

Description

A kind of nostoc milk tea, nostoc milk tea powder and preparation method
Technical field
The present invention relates to a kind of drink, particularly a kind of nostoc milk tea, nostoc milk tea powder and preparation method.
Background technology
Nostoc intends being commonly called as of spherical nostoc (NostocsphaeoidesK ü tzing) in Nostoc (NostocVauch), it belongs to Cyanophyta (Cyanophyta), Cyanophyceae (Cyanophyceae), beads Cutleriales (Nostocales), nostocaceae (Nostocaceae).Nostoc also known as green bird's nest, have clear liver and improve vision, effect of beauty treatment moisturizing, also precious rarer than delivering vegetables, glitter gloss as black pearl.In addition, it also has the feature of low-fat low-calorie, is applicable to weight reducing fat-reducing, can be rated as the king of lock water.Nostoc mouthfeel is lubricious, and shape circle look is beautiful, to arrange in pairs or groups the aesthetic feeling of its uniqueness and mouthfeel with any one snack food or beverage.
Moreover, containing 18 seed amino acids in nostoc, comprising eight seed amino acids of needed by human, dry total protein is up to about 56%, crude fat 8.11%, carbohydrate 12.69%, ash content 10.88%, chlorophyll 30.98mg/g, ascorbic acid 5.50mg/g, Vitamin C content is close to fresh jujube, higher than hawthorn more than 5 times, higher than citrus 5 times; Vitamin B1, B2 are higher than general Homonemeae; Be rich in mineral matter and trace element, also have phycobniliprotein and polysaccharide bioactive ingredients, its calcium content is higher than general vegetables simultaneously, is a kind of fabulous Natural Rich Calcium nutraceutical.
In addition, the medical value of nostoc is also precious.Compendium of Material Medica, " property of medicine is examined ", " national Chinese herbal medicine compilation " etc. claim effect of nostoc to be: improving eyesight benefit gas, made us son, antipyretic clearly every, sharp stomach, convulsive seizure due to phlegm-fire can treat, tool beautifying face and moistering lotion, improve the health, replenish the calcium, food anticancer, of a specified duration is prolonged life, dispelling fatigue, restrain, control yctalopia etc.Therefore, nostoc is genuine natural nutrition storehouse, can be rated as the jewelry on the tip of the tongue.
Milk tea has the double nutrition of milk and tea concurrently, not still one of homely cuisines, and the world in fashion.1680, York duchess introduced Britain by tea with the method for fresh milk and granulated sugar, and luxuriant is for the moment agitation, and spreads so far.In recent years, the kind of milk tea emerges in an endless stream, as Coconut Milk Tea, banana milk tea, warm ginger milk tea, rose milk tea, nut milk tea, India's milk tea, Mongolian milk, Xinjiang milk tea, grassland milk tea etc., various intention milk tea brings warm and satisfied to the life of people.Originate from the pearl milk tea in Taiwan, the powder circle that the pitch-black crystalline substance of outward appearance is saturating is added in milk tea, therefore name with " pearl ", separately can add the various batching such as pudding, coconut palm fruit, be modulated into flavour, deeply like by masses, imported into all over the world, only its trace is still not easily seen in European and American areas, and price is also relatively costly.But have not yet to see the relevant report adopting nostoc to prepare milk tea beverage as raw material.
Although also there is Nostoc commune beverage and nostoc juice beverage in prior art, there is lower column defects: (1) high-temperature process, the degraded of nostoc phycobniliprotein can be caused, thus cause nostoc variable color; (2) existing Nostoc commune beverage batching is single, does not focus on the adjustment of mouthfeel; (3) existing nostoc drink of being correlated with is not suitable for cold and cool physique crowd and eats in a large number.
In order to satisfied masses are to the needs of milk tea, be badly in need of a kind of nostoc milk tea beverage of preparation.
Summary of the invention
Instant invention overcomes in prior art the defect preparing nostoc drink, provide a kind of nostoc milk tea, nostoc milk tea powder and preparation method corresponding respectively.
In order to achieve the above object, the technical scheme that concrete employing is following: a kind of nostoc milk tea, comprises following component by weight: the tea extract of 20-30 part, the milk of 40-60 part, the nostoc phycobniliprotein crude extract of 10-20 part, the nostoc polysaccharide gel nutrient solution of 15-25 part, the flavor enhancement of 1-10 part, the sucrose fatty ester of 0.1-0.5 part, the citric acid of 0.1-0.4 part, the sweetener of 4-8 part, the honey of 5-8 part, 0.05-0.2 part thickener, 200 ~ 500 parts of water.
In order to increase nutrition and the aesthetic feeling of milk tea, make milk tea have the mouthfeel of Q bullet, nostoc milk tea of the present invention also comprises the nostoc fresh goods like " pearl ".
" pearl " in tradition pearl milk tea is although have certain nutritive value, but artificial forms, and is unfavorable for the chemical composition of health containing some, and its edible safety is controversial.And nostoc adopts natural water body cultivation, green health, non-harmful edible freshwater algae, not only form is mellow and full, outward appearance is beautiful, and nutritious, is a kind of raw-food material with health-care efficacy of pure natural.
" pearl " in the alternative traditional pearl milk tea of nostoc fresh goods in the present invention, not only nontoxic, delicious flavour, richly chew strength, and nutritious, be the alternative good merchantable brand of pearl in traditional pearl milk tea.
Nostoc fresh goods in the present invention can directly selected from nostoc fresh goods after clean up for subsequent use; Or be the nostoc dry product distilled water of 10-15% by moisture content or drink purified water and soak that selected wherein look black shape circle after its abundant rehydration and the pearl shape spheroid of mouthfeel Q bullet clean up for subsequent use.
Mellow and full for nostoc bright and new pearlite not only introduces in the water and milk system of milk tea by the present invention, and nostoc phycobniliprotein crude extract and nostoc polysaccharide gel nutrient solution (polysaccharide crude extract) are joined in milk tea as natural colouring matter and stabilizing agent, milk tea is not only made to be in bright colors, and mellow in taste, the more important thing is that there is unique medicinal health care function.
Meanwhile, the nostoc phycobniliprotein crude extract in the present invention has oxidation resistant effect, therefore in nostoc milk tea without the need to adding other antioxidant any, as VC, sodium iso-vc, VE etc.
In the present invention, nostoc phycobniliprotein crude extract can select commercially available all products.Preferably, following scheme can be adopted to be prepared:
(1) get nostoc fresh goods, cleaning, dries surface moisture, adopts the anhydrous nostoc fresh goods of multigelation method treatment surface;
(2) collect the bluish violet liquid discharged in step (1) and nostoc organism residue, described bluish violet liquid is nostoc phycobniliprotein crude extract.
In the preparation method of above-mentioned nostoc phycobniliprotein crude extract, concrete, the multigelation method in step (1) refers to, by nostoc fresh goods freezing 1-2 hour at-10 ~-4 DEG C, take out and put 5-10 minute in ambient temperatare, this process 3-5 time repeatedly.
The present invention utilizes nostoc polysaccharide gel nutrient solution as the pure natural thickener of milk tea, and not only mouthfeel is abundant, and fine and smooth smooth, the more important thing is also have high medicinal health care function.In addition nostoc polysaccharide gel nutrient solution also has antibacterial and antisepsis significantly, can be used as Pure natural preservative, substitutes traditional anticorrisive agent.
In the present invention, nostoc polysaccharide gel nutrient solution can select commercially available all products.Preferably, following scheme can be adopted to be prepared:
(1) with the nostoc organism residue 1.5-2.5h that the NaOH solution of 0.1 ~ 0.5mol/L produces in 35-45 DEG C of process nostoc phycobniliprotein coarse body fluid preparation process, then neutrality is neutralized to citric acid;
(2) in step (1) gained solution, add papain according to the ratio of every 100mg material interpolation 0.5-1.0mg papain, at 40-45 DEG C, extract 8-10h, through alkali-enzyme Combined Processing, obtain extract;
(3) extract that step (2) obtains is concentrated into the 1/4-1/3 that volume is original volume, obtains nostoc polysaccharide gel nutrient solution.
In the preparation method of above-mentioned nostoc polysaccharide gel nutrient solution, the extract of step (2) gained adopts phend-sulphuric acid to measure its total sugar content can reach 13%-22%.
Step (3) employing vacuumizes heating and concentrates.
The extraction process of nostoc phycobniliprotein crude extract and nostoc polysaccharide gel nutrient solution combines by the present invention, first from raw material, obtain nostoc phycobniliprotein crude extract, from waste material (nostoc organism residue), obtain nostoc polysaccharide gel nutrient solution again, substantially increase the raw-material utilization rate of nostoc.
Concrete in nostoc milk tea, described flavor enhancement is selected from one or more in peanut powder, Iodine Cardia Salt, rare cream, chocolate powder, preferred rare cream; Described thickener is selected from one or more in maltodextrin, agar, xanthans, preferred maltodextrin; Described sweetener be selected from xylitol, white granulated sugar one or both, preferred xylitol.
Because nostoc feeding habits are cold and cool, therefore the crowd of cold of insufficiency type physique should not eat in a large number.Adopt the method mixed with the Chinese medicine of heat warm in nature by nostoc not only can overcome the cold and cool shortcoming of nostoc, also can realize treatment and prophylactic effect, so also added the nourishing auxiliary material of 0.01-0.2 part in milk tea of the present invention
Described nourishing auxiliary material is syrup, and the raw material of described syrup comprises matrimony vine 12-20 part by weight, Radix Astragali 5-8 part, date 10-20 part, the white granulated sugar of 17-35 part, the dextrin of 2-7 part, the water of 50-100 part.The preparation method of syrup is preparation method conventional in prior art.
The preparation method of nostoc milk tea of the present invention, comprises the following steps:
(1) in the milk of abundant preheating, sucrose fatty ester, nostoc polysaccharide gel nutrient solution, flavor enhancement, thickener, sweetener, honey, citric acid is added, stir after making it fully dissolve and mix with the tea extract of preheating while hot, stir after making it mix and be cooled to temperature≤35 DEG C;
(2) in the mixture of step (1) gained, add nostoc phycobniliprotein crude extract, adopt colloid mill to carry out homogeneous to this water and milk system;
(3) supply water, obtain nostoc milk tea; Or add nourishing auxiliary material or nostoc fresh goods in one or both, supply water, obtain nostoc milk tea.
The preparation method of nostoc milk tea provided by the invention makes nostoc phycobniliprotein crude extract farthest maintain activity.
In order to reach another object of the present invention, the technical scheme that concrete employing is following: a kind of nostoc milk tea powder, comprises following component by weight: the instant tea powder of 0.5-1 part, the milk powder of 10-25 part, the nostoc phycobniliprotein of 0.1-0.5 part slightly carries powder, the nostoc polysaccharide dry powder of 0.1-1, the flavor enhancement of 1-10 part, the sucrose fatty ester of 0.1-0.5 part, the citric acid of 0.1-0.4 part, the sweetener of 4-8 part, the honey of 5-8 part, the thickener of 0.05-0.2 part.
Nostoc milk tea powder of the present invention can also comprise nostoc dry product.Wherein, the milk powder in the present invention can be whole milk powder or skimmed milk power.
The moisture content 10-15% of nostoc dry product.
Concrete in nostoc milk tea powder, described flavor enhancement is selected from one or more in peanut powder, Iodine Cardia Salt, dry cream, chocolate powder, preferred cream powder; Described thickener is selected from one or more in maltodextrin, agar, xanthans, preferred maltodextrin; Described sweetener is selected from one or more in xylitol, white granulated sugar, preferred xylitol.
Nostoc milk tea powder provided by the invention is easy to carry, makes simple, and nutritious, and the nostoc milk tea utilizing nostoc milk tea powder of the present invention to prepare has the bright luster of the nostoc liquid milk tea that the present invention is directly prepared, mouthfeel and nutrition.
Nostoc phycobniliprotein of the present invention slightly carries powder can select commercially available all products.In addition, nostoc phycobniliprotein runic powder can utilize nostoc phycobniliprotein crude extract of the present invention to prepare for raw material, specifically comprises the following steps:
(1) get nostoc phycobniliprotein crude extract high speed refrigerated centrifuge at 4-10 DEG C, abandon precipitation;
(2) adding ammonium sulfate in the supernatant after centrifugal to step (1) makes it reach 20%-30% saturation degree, repeats the centrifugally operated of step (1), abandons precipitation;
(3) adding ammonium sulfate in the supernatant after centrifugal to step (2) makes it reach the saturation degree of 50%-60%, repeats centrifugally operated in step (1), abandons supernatant, obtain sediment;
(4) in 3-5 DEG C of dialysis after step (3) gained sediment being dissolved with phosphate buffer;
(5) the precipitation phosphate buffer after dialysis dissolves, and the thick phycobniliprotein namely obtaining nostoc after vacuum freeze drying slightly carries powder.
Slightly carry in the preparation method of powder at above-mentioned nostoc phycobniliprotein, concrete, time centrifugal in step (1), rotating speed is 10000-14000r/min, and centrifugation time is 30-45min.
Adding ammonium sulfate in step (2) makes it reach 20% saturation degree.
Adding ammonium sulfate in step (3) makes it reach the saturation degree of 50%.
Preferably, the ammonium sulfate added in step (2) and step (3) is solid.
In step (5), cryodesiccated condition is-10 ~-20 DEG C, vacuum 1.3-13 handkerchief, and sublimation temperature is 20-30 DEG C.
The compound method of phosphate buffer is by the Na of 0.2M 2hPO 412H 2the NaH of O solution 61.0ml and 0.2M 2pO 42H 2the solution 39.0ml of O, mixes.
Nostoc polysaccharides dry powder of the present invention can select commercially available all products.In addition, nostoc polysaccharide dry powder can utilize nostoc polysaccharide gel nutrient solution of the present invention to prepare for raw material, specifically comprises the following steps:
(1) ethanol is mixed with nostoc polysaccharide gel nutrient solution, make concentration of alcohol reach 70-80%, namely occur nostoc polysaccharide precipitation;
(2) centrifugal recovery precipitation, obtains nostoc polysaccharide dry product through vacuum freeze drying, obtains nostoc polysaccharide dry powder after pulverizing.
In the preparation method of above-mentioned nostoc polysaccharide dry powder, the ethanol of step (1) is absolute ethyl alcohol or 95% (mass ratio) ethanol.
Adopt pulverizer to be ground into 150-250 object fine powder nostoc polysaccharide dry product in step (2), namely obtain nostoc polysaccharide dry powder.
In step (2), cryodesiccated condition is-10 ~-20 DEG C, vacuum 1.3-13 handkerchief, and sublimation temperature is 20-30 DEG C.
Because nostoc feeding habits are cold and cool, therefore the crowd of cold of insufficiency type physique should not eat in a large number.Adopt the method mixed with the Chinese medicine of heat warm in nature by nostoc not only can overcome the cold and cool shortcoming of nostoc, also can realize treatment and prophylactic effect, so also added the nourishing auxiliary material of 0.01-0.2 part in milk tea powder of the present invention.
Described nourishing auxiliary material is syrup, and the raw material of described syrup comprises matrimony vine 12-20 part by weight, Radix Astragali 5-8 part, date 10-20 part, the white granulated sugar of 17-35 part, the dextrin of 2-7 part, the water of 50-100 part.The preparation method of syrup is preparation method conventional in prior art.
The preparation method of nostoc milk tea powder of the present invention, comprises the following steps:
(1) by instant tea powder, milk powder, sucrose fatty ester, citric acid, flavor enhancement, thickener, sweetener mixing, at the circulated under pressure homogeneous of 30-34MPa, then ultra-high pressure sterilization 10-30min under 400 ~ 600MPa, adopts the method for vacuum nitrogen filling gas to pack it;
(2) adopt that the method for vacuum nitrogen filling gas slightly carries powder to nostoc phycobniliprotein respectively, nostoc Thick many candies dry powder, honey, nostoc dry product are packed;
(3) according to step (1) made pulvis: nostoc phycobniliprotein slightly carries powder: nostoc Thick many candies dry powder: honey=1 bag: 1 bag: 1 bag: 1 bag, milky tea cup is loaded; Or add further 1 bag of nostoc dry product or 1 bag nourishing auxiliary material one or both, load milky tea cup, seal and get final product.
Illustrate: in the preparation method of nostoc milk tea powder of the present invention, because nostoc dry product or nourishing auxiliary material are the components can selecting to add, instead of the component that must add, refer to adding further in step (3) in the raw material of nostoc milk tea powder containing nostoc dry product or nourishing auxiliary material time, add in step (3).
First with the drinking water of temperature, nostoc dry product bubble can be sent out when nostoc milk tea powder of the present invention eats, make its abundant rehydration stand-by; The pulvis hot drink purified water of preparation in step (1) is dissolved simultaneously, while hot nostoc Thick many candies dry powder is dissolved, stir and evenly mix cooling, slightly carry powder with nostoc phycobniliprotein again, honey dissolves, stir and evenly mix, then add the nostoc like " pearl " or the nourishing auxiliary material of abundant rehydration wherein, personal like looked by general nourishing auxiliary material, the product user of cold of insufficiency type physique, suggestion is edible after adding.
And in the present invention, in extraction and milk tea preparation process, the control focusing on process conditions keeps the activity of phycobniliprotein and polysaccharide, particularly by control temperature with first put forward order that phycobniliprotein extracts polysaccharide again and keep the activity of phycobniliprotein, first cool the process modification such as interpolation again during preparation, overcome the defect of high temperature phycobniliprotein activity in other relevant nostoc drink.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1-embodiment 6
Embodiment 1-embodiment 6 is the raw material composition of nostoc milk tea, specifically as shown in table 1:
Table 1: the composition (g) of embodiment 1-embodiment 6 nostoc milk tea
Embodiment 7-embodiment 12
Embodiment 7-embodiment 12 is the raw material composition of nostoc milk tea powder, specifically as shown in table 2:
Table 2: the composition (g) of embodiment 7-embodiment 12 nostoc milk tea powder
Embodiment 13
In embodiment 1-6, the preparation method of nostoc milk tea, specifically comprises the steps:
(1) in the milk of abundant preheating, sucrose fatty ester, nostoc polysaccharide gel nutrient solution, flavor enhancement, thickener, sweetener, honey, citric acid is added, stir after making it fully dissolve and mix with the tea extract of preheating while hot, stir after making it mix and be cooled to temperature≤35 DEG C;
(2) in the mixture of step (1) gained, add nostoc phycobniliprotein crude extract, adopt colloid mill to carry out homogeneous to this water and milk system;
(3) supply water, obtain nostoc milk tea; Or add nourishing auxiliary material or nostoc fresh goods in one or both, supply water, obtain nostoc milk tea.
Embodiment 14
The preparation method of nostoc milk tea powder in embodiment 7-12, comprises the following steps:
(1) by instant tea powder, milk powder, sucrose fatty ester, citric acid, flavor enhancement, thickener, sweetener mixing, again at the circulated under pressure homogeneous of 30-34MPa, then ultra-high pressure sterilization 10-30min under 400 ~ 600MPa, finally adopts the method for vacuum nitrogen filling gas to pack it;
(2) adopt that the method for vacuum nitrogen filling gas slightly carries powder to nostoc phycobniliprotein respectively, nostoc Thick many candies dry powder, honey, nostoc dry product are packed;
(3) according to step (1) made pulvis: nostoc phycobniliprotein slightly carries powder: nostoc Thick many candies dry powder: honey=1 bag: 1 bag: 1 bag: 1 bag, milky tea cup is loaded; Or add further 1 bag of nostoc dry product or 1 bag nourishing auxiliary material one or both, load milky tea cup, seal and get final product.
Embodiment 15
The present embodiment is the preparation of nostoc phycobniliprotein crude extract/slightly carry powder, nostoc polysaccharide gel nutrient solution/polysaccharide dry powder.
1, the preparation of nostoc phycobniliprotein crude extract
(1) get nostoc fresh goods, cleaning, dries surface moisture, adopt the anhydrous nostoc fresh goods of multigelation method treatment surface, concrete operation method is by nostoc fresh goods freezing 1-2 hour at-10 ~-4 DEG C, takes out and puts 5-10 minute in ambient temperatare, this process 3-5 time repeatedly;
(2) collect the bluish violet liquid discharged in step (1) and nostoc organism residue, described bluish violet liquid is nostoc phycobniliprotein crude extract.
2. nostoc phycobniliprotein slightly proposes the preparation of powder
(1) get nostoc phycobniliprotein crude extract high speed refrigerated centrifuge at 4-10 DEG C, time centrifugal, rotating speed is 10000-14000r/min, and centrifugation time is 30-45min, abandons precipitation;
(2) adding solid ammonium sulfate in the supernatant after centrifugal to step (1) makes it reach 20% saturation degree, repeats the centrifugally operated of step (1), abandons precipitation;
(3) adding solid ammonium sulfate in the supernatant after centrifugal to step (2) makes it reach the saturation degree of 50%, repeats centrifugally operated in step (1), abandons supernatant, obtain sediment;
(4) in 4 DEG C of dialysis after step (3) gained sediment being dissolved with phosphate buffer;
(5) precipitation after dialysis is dissolved with a small amount of phosphate buffer, namely obtains the thick phycobniliprotein powder of nostoc after vacuum freeze drying.Concrete cryodesiccated condition is-10 ~-20 DEG C, and vacuum 1.3-13 handkerchief, sublimation temperature is 20-30 DEG C.
Concrete, in the preparation method of the thick phycobniliprotein powder of above-mentioned nostoc, the compound method of phosphate buffer is by 0.2MNa 2hPO 412H 2o solution 61.0ml and 0.2MNaH 2pO 42H 2o solution 39.0ml, mixes.
3, the preparation of nostoc polysaccharide gel nutrient solution
(1) with the nostoc organism residue 2h that the NaOH solution of 0.1 ~ 0.5mol/L produces in 40 DEG C of process nostoc phycobniliprotein coarse body fluid preparation process, then neutrality is neutralized to citric acid;
(2) in step (1) gained solution, papain is added according to the ratio of every 100mg material interpolation 0.5-1.0mg papain, 8-10h is extracted at 40-45 DEG C, through alkali-enzyme Combined Processing, obtain extract, adopting phend-sulphuric acid to measure its total sugar content can reach 13%-22%;
(3) extract that step (2) obtains is concentrated into the 1/3-1/4 that volume is original volume, obtains nostoc polysaccharide gel nutrient solution.
4, the preparation of nostoc polysaccharide dry powder
(1) with 95% ethanol or the mixing of nostoc polysaccharide gel nutrient solution, make concentration of alcohol reach 70-80%, namely have cotton-shaped Thick many candies to precipitate, leave standstill;
(2) freeze drying of centrifugal recovery precipitation final vacuum obtains Thick many candies dry product, adopts pulverizer to be ground into 200 order fine powders.
Experimental example
This experimental example, according to beverage sensory evaluation standard, carries out sensory evaluation to nostoc milk tea prepared by embodiment 1-12 respectively.Taster is tasted when beverage the unknown, each taster all carries out objective scoring according to standards of grading, finally get the intermediate value of all scorings as Appreciation gist, selected trial test number is each 60 people of men and women, comprising each 20 of the men and women of 15-23 year, 24-35 year, 36-45.
According to the characteristic of nostoc and the basic index of judge beverage mouthfeel, be set as follows evaluation standard, as shown in the table.
Evaluation standard
Evaluating result is as shown in the table:
Sample Taste Smell Impurity State Comprehensive grading
Embodiment 1 42 25 13 9 89 points
Embodiment 2 43 27 12 10 92 points
Embodiment 3 44 24 14 8 90 points
Embodiment 4 40 23 13 10 86 points
Embodiment 5 41 25 15 8 89 points
Embodiment 6 45 25 13 9 92 points
Embodiment 7 40 24 15 8 87 points
Embodiment 8 42 26 12 9 89 points
Embodiment 9 41 28 12 9 90 points
Embodiment 10 43 20 15 8 86 points
Embodiment 11 45 24 11 9 89 points
Embodiment 12 44 21 14 9 88 points
Embodiment illustrates: the nostoc milk tea obtained according to the composition proportion preparation of embodiment 1-12, through the objective mouthfeel test and appraisal of experimental example, and liking extensively by each age group taster.Carry out objective mouthfeel to all nostoc milk tea obtain according to the preparation of the minimum of each composition of nostoc milk tea, median and peak proportioning to test and assess.From mouthfeel evaluating result, the milk tea adopting the inventive method to prepare all achieves higher score, meets popular taste, by masses are approved.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.

Claims (10)

1. a nostoc milk tea, is characterized in that, comprises following component by weight: the tea extract of 20-30 part, the milk of 40-60 part, the nostoc phycobniliprotein crude extract of 10-20 part, the nostoc polysaccharide gel nutrient solution of 15-25 part, the flavor enhancement of 1-10 part, the sucrose fatty ester of 0.1-0.5 part, the citric acid of 0.1-0.4 part, the sweetener of 4-8 part, the honey of 5-8 part, 0.05-0.2 part thickener, 200 ~ 500 parts of water;
Described nostoc phycobniliprotein crude extract adopts following scheme to be prepared:
(1) get nostoc fresh goods, cleaning, dries surface moisture, adopts the anhydrous nostoc fresh goods of multigelation method treatment surface;
(2) collect the bluish violet liquid discharged in step (1) and nostoc organism residue, described bluish violet liquid is nostoc phycobniliprotein crude extract;
Described nostoc polysaccharide gel nutrient solution adopts following scheme to be prepared:
(1) with the nostoc organism residue 1.5-2.5h that the NaOH solution of 0.1 ~ 0.5mol/L produces in 35-45 DEG C of process nostoc phycobniliprotein coarse body fluid preparation process, then neutrality is neutralized to citric acid;
(2) in step (1) gained solution, add papain according to the ratio of every 100mg material interpolation 0.5-1.0mg papain, at 40-45 DEG C, extract 8-10h, through alkali-enzyme Combined Processing, obtain extract;
(3) extract that step (2) obtains is concentrated into the 1/4-1/3 that volume is original volume, obtains nostoc polysaccharide gel nutrient solution;
Described flavor enhancement is selected from one or more in peanut powder, Iodine Cardia Salt, rare cream, chocolate powder; Described thickener is selected from one or more in maltodextrin, agar, xanthans; Described sweetener be selected from xylitol, white granulated sugar one or both.
2. nostoc milk tea according to claim 1, it is characterized in that: also comprise nostoc fresh goods, clean up for subsequent use after described nostoc fresh goods is directly selected from nostoc fresh goods or be the nostoc dry product distilled water of 10-15% by moisture content or drink purified water and soak, selected look black shape circle after abundant rehydration and the pearl shape spheroid of mouthfeel Q bullet.
3. nostoc milk tea according to claim 1, is characterized in that: rare cream chosen by described flavor enhancement; Maltodextrin chosen by described thickener; Described sweetener chooses xylitol.
4. the nostoc milk tea according to any one of claim 1-3, it is characterized in that: the nourishing auxiliary material syrup also comprising 0.01-0.2 part, the raw material of described syrup comprises the matrimony vine of 12-20 part by weight, the Radix Astragali of 5-8 part, the date of 10-20 part, the white granulated sugar of 17-35 part, the dextrin of 2-7 part, the water of 50-100 part.
5. the preparation method of nostoc milk tea according to any one of claim 1-4, is characterized in that, comprise the following steps:
(1) in the milk of abundant preheating, sucrose fatty ester, nostoc polysaccharide gel nutrient solution, flavor enhancement, thickener, sweetener, honey, citric acid is added, stir after making it fully dissolve and mix with the tea extract of preheating while hot, stir after making it mix and be cooled to temperature≤35 DEG C;
(2) in the mixture of step (1) gained, add nostoc phycobniliprotein crude extract, adopt colloid mill to carry out homogeneous to this water and milk system;
(3) supply water, obtain nostoc milk tea; Or add nourishing auxiliary material or nostoc fresh goods in one or both, supply water, obtain nostoc milk tea.
6. a nostoc milk tea powder, it is characterized in that, comprise following component by weight: the instant tea powder of 0.5-1 part, the milk powder of 10-25 part, the nostoc phycobniliprotein of 0.1-0.5 part slightly carries powder, the nostoc polysaccharide dry powder of 0.1-1, the flavor enhancement of 1-10 part, the sucrose fatty ester of 0.1-0.5 part, the citric acid of 0.1-0.4 part, the sweetener of 4-8 part, the honey of 5-8 part, the thickener of 0.05-0.2 part;
Described nostoc phycobniliprotein is slightly carried powder and is adopted following scheme to be prepared:
(1) get nostoc phycobniliprotein crude extract high speed refrigerated centrifuge at 4-10 DEG C, abandon precipitation;
(2) adding ammonium sulfate in the supernatant after centrifugal to step (1) makes it reach 20%-30% saturation degree, repeats the centrifugally operated of step (1), abandons precipitation;
(3) adding ammonium sulfate in the supernatant after centrifugal to step (2) makes it reach the saturation degree of 50%-60%, repeats centrifugally operated in step (1), abandons supernatant, obtain sediment;
(4) in 3-5 DEG C of dialysis after step (3) gained sediment being dissolved with phosphate buffer;
(5) the precipitation phosphate buffer after dialysis dissolves, and the thick phycobniliprotein namely obtaining nostoc after vacuum freeze drying slightly carries powder;
Described nostoc polysaccharide dry powder adopts following scheme to be prepared:
(1) with the nostoc organism residue 1.5-2.5h that the NaOH solution of 0.1 ~ 0.5mol/L produces in 35-45 DEG C of process nostoc phycobniliprotein coarse body fluid preparation process, then neutrality is neutralized to citric acid;
(2) in step (1) gained solution, add papain according to the ratio of every 100mg material interpolation 0.5-1.0mg papain, at 40-45 DEG C, extract 8-10h, through alkali-enzyme Combined Processing, obtain extract;
(3) extract that step (2) obtains is concentrated into the 1/4-1/3 that volume is original volume, obtains nostoc polysaccharide gel nutrient solution;
(4) ethanol is mixed with nostoc polysaccharide gel nutrient solution, make concentration of alcohol reach 70-80%, namely occur nostoc polysaccharide precipitation;
(5) centrifugal recovery precipitation, obtains nostoc polysaccharide dry product through vacuum freeze drying, obtains nostoc polysaccharide dry powder after pulverizing;
Described flavor enhancement is selected from one or more in peanut powder, Iodine Cardia Salt, dry cream, chocolate powder; Described thickener is selected from one or more in maltodextrin, agar, xanthans; Described sweetener be selected from xylitol, white granulated sugar one or both.
7. nostoc milk tea powder according to claim 6, is characterized in that: also comprise nostoc dry product.
8. nostoc milk tea powder according to claim 6, is characterized in that: dry cream chosen by described flavor enhancement; Maltodextrin chosen by described thickener; Described sweetener chooses xylitol.
9. the nostoc milk tea powder according to any one of claim 6-8, it is characterized in that: the nourishing auxiliary material syrup also comprising 0.01-0.2 part, the raw material of described syrup comprises the matrimony vine of 12-20 part by weight, the Radix Astragali of 5-8 part, the date of 10-20 part, the white granulated sugar of 17-35 part, the dextrin of 2-7 part, the water of 50-100 part.
10. the preparation method of nostoc milk tea powder according to any one of claim 6-9, is characterized in that, comprise the following steps:
(1) by instant tea powder, milk powder, sucrose fatty ester, citric acid, flavor enhancement, thickener, sweetener mixing, at the circulated under pressure homogeneous of 30-34MPa, then ultra-high pressure sterilization 10-30min under 400 ~ 600MPa, adopts the method for vacuum nitrogen filling gas to pack it;
(2) adopt that the method for vacuum nitrogen filling gas slightly carries powder to nostoc phycobniliprotein respectively, nostoc Thick many candies dry powder, honey, nostoc dry product are packed;
(3) according to step (1) made pulvis: nostoc phycobniliprotein slightly carries powder: nostoc Thick many candies dry powder: honey=1 bag: 1 bag: 1 bag: 1 bag, milky tea cup is loaded; Or add further 1 bag of nostoc dry product or 1 bag nourishing auxiliary material in one or both, load milky tea cup, seal and get final product.
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