JP2003095856A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a skin care preparation comprising an extract from Cummiphora mukul, Beriberis aristata and Pterocarpas marsapim. SOLUTION: The extract from the Cummiphora mukul, Beriberis aristata and Pterocarpas marsapim has excellent active oxygen scavenging actions, hyaluronidase inhibitory, elastase inhibitory and collagenase inhibitory actions and is stable. Furthermore, the skin care preparation comprising the Cummiphora mukul, Beriberis aristata and Pterocarpas marsapim exhibits high safety and excellent antiaging actions.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin which is excellent in preventing aging by containing one or more kinds of extracts selected from Indian Buderium tree, Indian barberry and Indian quino tree. .

[0002]

2. Description of the Related Art In recent years, free radicals and active oxygen have been taken up as factors that oxidize biological components, and their adverse effects have become a problem. It is said that free radicals and active oxygen are generated in the living body to decompose or cross-link living tissues such as collagen and to oxidize oils and fats to form lipid peroxides that damage cells. Such disorders are considered to cause aging such as wrinkles and firmness of the skin, and one of the methods for preventing aging is to add an antioxidant that removes free radicals and active oxygen. Are known. Conventionally, as free radical scavengers used for the purpose of preventing aging, ascorbic acid, tocopherol, 3,5-tert-butyl-4-hydroxytoluene (BHT), superoxide dismutase (SO
D) and the like have been used.

The skin consists of epidermis, dermis and subcutaneous tissue. Among them, the dermis is extremely important for maintaining the structure of the skin,
The firmness of the skin is maintained by the dermal connective tissue formed of hyaluronic acid, elastin, collagen and the like. It is believed that this connective tissue loses its contractile force and further loses its elasticity, resulting in wrinkles and tarmi on the skin.

It is also known that matrix metalloproteases such as elastase and collagenase are activated when the skin is exposed to ultraviolet rays. These enzymes are
It is said to promote wrinkles and tarmi by reducing elastin and collagen, which are the main components of the dermis. Hyaluronidase is also activated during inflammation,
It is known that by lowering the molecular weight of high molecular weight hyaluronic acid, the firmness of the skin is reduced and aging such as wrinkles is caused.

In recent years, many cosmetics for preventing such wrinkles and tarmi on the skin are known, and retinoic acid, α-hydroxy acid, retinol and the like have been reported as the active ingredients thereof. However, these active ingredients have problems with skin irritation and stability. Further, it is known to mix hyaluronidase, elastase and collagenase inhibitor as one of the methods for preventing wrinkles and tarmi. However, as a plant material having an inhibitory effect on these enzymes, the plant of the genus Alderia has not been studied.

[0006]

SOD used for the purpose of preventing skin aging or antioxidant is unstable and difficult to formulate, and tocopherol cannot be said to be sufficiently effective. Further, since BHT and the like which are synthetic compounds have a safety problem and the compounding amount is limited, a natural raw material that is stable and has few side effects is desired, not a chemically synthesized product. Similarly, it is preferable to have a safe and stable hyaluronidase, elastase and collagenase inhibitory action for preventing aging.

From the above, there is a demand for a skin external preparation that is safe, has excellent stability, and is excellent in preventing aging.

[0008]

[Means for Solving the Problems] Due to such circumstances,
As a result of intensive studies by the present inventors, the extracts of Indian Buderium tree, Indian barberry and Indian quino tree have excellent active oxygen scavenging activity, hyaluronidase inhibition, elastase inhibition, collagenase inhibition and tyrosinase inhibitory activity, and stability. It was found to be excellent in. Furthermore, they have found that an external preparation for skin containing the extract is safe and stable and is excellent in preventing aging, and completed the present invention.

Indian Buderium tree (Orchidaceae, Scientific name: Cummiphora muk) used in the present invention
ul), the resin has been used in India and Nepal for folk arthritis, neuralgia, hypertension, etc. Also,
Indian burberry (Barberry family, scientific name Biber)
is aristata) is used for its fruits, bark, root melena, eye diseases, etc., and Indian quino tree (Fabaceae, Scientific name: Pterocarpas marsapi)
In m), the seeds have been used for anti-inflammatory, hemostasis, skin diseases, and bruising. In addition, these plants can be obtained and used as crude drugs from regions such as India and Southeast Asia.

The Indian Buderium tree, Indian barberry and Indian quino tree extracts used in the present invention include plant leaves, stems, bark, flowers, fruits,
It is extracted from a part or whole plant of a plant such as seeds and roots. Preferable ones are those obtained by extraction from Indian Buderium tree resin and bark, Indian barberry fruit and bark, and Indian quino tree seed and resin. The extraction method is not particularly limited, and for example,
It may be one extracted by heating or one extracted at room temperature.

The solvent to be extracted is, for example, water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohol (1,3-butylene glycol). , Propylene glycol, glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran) , Propyl ether, etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.

The extract may be used as it is as an extracted solution, or may be concentrated, diluted, filtered, decolorized with activated carbon, deodorized and the like, if necessary. Further, the extracted solution may be concentrated to dryness, spray-dried, freeze-dried and the like and used as a dried product.

In the external preparation for skin of the present invention, the above extract may be used as it is, and as long as the effect of the extract is not impaired, fats and oils, waxes which are components used in the external preparation,
Hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickeners, pigments, antioxidants,
Ingredients such as a whitening agent and a chelating agent can be added.

The external preparation for skin of the present invention can be used in any of cosmetics, quasi drugs, and pharmaceuticals. Examples of its dosage form include lotion, cream, emulsion, gel, and aerosol. , Essences, packs, cleansing agents, bath agents, foundations, dusting powders, lipsticks, ointments, poultices and the like applied to the skin.

The amount of the above extract used in the present invention is 0.0 based on the total amount of the skin external preparation of the present invention in terms of solid matter.
001% by weight or more, preferably 0.001 to 10% by weight
Is a good combination. If it is less than 0.0001% by weight, it is difficult to expect a sufficient effect. When it is blended in an amount exceeding 10% by weight, it is uneconomical since the enhancement of the effect is hardly recognized. Regarding the method of addition, it may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

[0016]

EXAMPLES In order to explain the present invention in detail, production examples of the extract used in the present invention, prescription examples of the present invention and experimental examples will be given below, but the present invention is not limited thereto. Absent. The parts of the compounding amounts shown in the examples are parts by weight, and% is% by weight.

Production Example 1 Hot water extract of Indian Buderium tree To 40 g of dried Indian Buderium tree resin, 800 mL of purified water was added, extracted at 95 to 100 ° C. for 2 hours, filtered, and the filtrate was concentrated. Then, the mixture was freeze-dried to obtain 8.8 g of a hot water extract of Indian Buderium tree.

Production Example 2 Ethanol extract of Indian Buderium tree 100 g of dried Indian Buderium tree resin
Ethanol (1 L) was added to the mixture, and the mixture was extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 4.2 g of an ethanol extract of Indian Buderium tree.

Preparation Example 3 50% 1,3-butylene glycol extract of Indian Buderium tree 20 g of dried bark of Indian Buderium tree, 200 mL of purified water and 20% of 1,3-butylene glycol
0 mL was added, and the mixture was extracted at room temperature for 7 days and then filtered to obtain 360 g of a 50% 1,3-butylene glycol extract of Indian Buderium tree.

Production Example 4 Indian barberry hot water extract 40 g of dried Indian barberry bark and fruit
Purified water (800 mL) was added thereto, and the mixture was extracted at 95 to 100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized to obtain 1.4 g of a hot water extract of Indian barberry.

Production Example 5 Hot water extract of Indian quino tree 2 L of purified water was added to 100 g of dried bark of Indian quino tree, and the mixture was extracted at 95 to 100 ° C. for 2 hours, filtered, and the filtrate was concentrated. It was freeze-dried to obtain 2.6 g of a hot water extract of Indian quino tree.

Production Example 6 50 of Indian Kino Tree
% 1,3-butylene glycol extract 20 g of dried Indian quino tree seeds and resin
Purified water 200mL and 1,3-butylene glycol 2
00 mL was added, and the mixture was extracted at room temperature for 7 days and then filtered to obtain 360 g of 50% 1,3-butylene glycol extract of Indian quino tree.

Example 1 Lotion Formulation Amount 1. Hot water extract of Indian Buderium tree (Production Example 1) 0.1 part 2.1,3-butylene glycol 8.0 3. Glycerin 2.0 4. Xanthan gum 0.02 5. Citric acid 0.01 6. Sodium citrate 0.1 7. Ethanol 5.0 8. Methyl paraoxybenzoate 0.1 9. Polyoxyethylene hydrogenated castor oil (40 EO) 0.1 10. Perfume proper amount 11. [Production method] Components 1 to 6 and 11 and components 7 to 10 each having a total amount of 100 with purified water are uniformly dissolved, and both are mixed and filtered to obtain a product.

Comparative Example 1 Conventional Lotion A conventional lotion was prepared by replacing the hot water extract of Indian Buderium tree with purified water in Example 1.

Example 2 Cream Formulation Amount 1. Ethanol extract of Indian Buderium tree (Production Example 2) 0.05 parts 2. Squalane 5.5 3. Olive oil 3.0 4. Stearic acid 2.0 5. Beeswax 2.0 6. Octyldodecyl myristate 3.5 7. Polyoxyethylene cetyl ether (20 EO) 3.0 8. Behenyl alcohol 1.5 9. Glycerin monostearate 2.5 10. Perfume 0.1 11. Methyl paraoxybenzoate 0.2 12. Ethyl paraoxybenzoate 0.05 13.1,3-Butylene glycol 8.5 14. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 9 are dissolved by heating and mixed, and the mixture is heated to 70 ° C.
Keep it in the oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. Add the water phase to the oil phase to emulsify, cool with stirring and mix at 10
Is added, and the product is further cooled to 30 ° C.

Comparative Example 2 A conventional cream was prepared by replacing the ethanol extract of Indian Buderium tree with purified water in Example 2 of the conventional cream.

Example 3 Emulsion formulation Compounding amount 1. Hot water extract of Indian Buderium tree (Production Example 1) 0.001 part 2. Squalane 5.0 3. Olive oil 5.0 4. Jojoba oil 5.0 5. Cetanol 1.5 6. Glycerin monostearate 2.0 7. Polyoxyethylene cetyl ether (20 EO) 3.0 8. Polyoxyethylene sorbitan monooleate (20EO) 2.0 9. Perfume 0.1 10. Propylene glycol 1.0 11. Glycerin 2.0 12. Methyl paraoxybenzoate 0.2 13. The total amount is 100 with purified water [Production method] Components 2 to 8 are dissolved by heating and mixed, and the mixture is heated to 70 ° C.
Keep it in the oil phase. Ingredients 1 and 10 to 13 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The water phase is added to the oil phase to emulsify, and the mixture is cooled with stirring, component 9 is added at 45 ° C, and further cooled to 30 ° C to obtain a product.

Example 4 Gel formulation Formulation amount 1. 50% 1,3-butylene glycol extract of Indian Buderium tree (Production Example 3) 1.0 part 2. Ethanol 5.0 3. Methyl paraoxybenzoate 0.1 4. Polyoxyethylene hydrogenated castor oil (60 EO) 0.1 5. Fragrance suitable amount 6.1,3-butylene glycol 5.0 7. Glycerin 5.0 8. Xanthan gum 0.1 9. Carboxyvinyl polymer 0.2 10. Potassium hydroxide 0.2 11. [Production method] Components 2 to 5 and components 1 and 6 to 11 each having a total amount of 100 with purified water are uniformly dissolved, and both are mixed to obtain a product.

[0029]   Example 5 Pack Prescription amount   1. Hot water extract of Indian barberry (Production Example 4) 0.1 part   2. Hot water extract of Indian Kino Tree (Production Example 5) 0.1   3. Polyvinyl alcohol 12.0   4. Ethanol 5.0   5.1,3-butylene glycol 8.0   6. Methyl paraoxybenzoate 0.2   7. Polyoxyethylene hydrogenated castor oil (20EO) 0.5   8. Citric acid 0.1   9. Sodium citrate 0.3 10. Fragrance suitable amount 11. Adjust the total volume to 100 with purified water [Production method] Components 1 to 11 are uniformly dissolved to obtain a product.

Example 6 Foundation prescription compounding amount 1. Hot water extract of Indian Kino Tree (Production Example 5) 1.0 part 2. Stearic acid 2.4 3. Polyoxyethylene sorbitan monostearate (20EO) 1.0 4. Polyoxyethylene cetyl ether (20 EO) 2.0 5. Cetanol 1.0 6. Liquid lanolin 2.0 7. Liquid paraffin 3.0 8. Isopropyl myristate 6.5 9. Carboxymethyl cellulose sodium 0.1 10. Bentonite 0.5 11. Propylene glycol 4.0 12. Triethanolamine 1.1 13. Methyl paraoxybenzoate 0.2 14. Titanium dioxide 8.0 15. Talc 4.0 16. Red iron oxide 1.0 17. Yellow iron oxide 2.0 18. Perfume proper amount 19. [Production method] Components 2 to 8 having a total amount of 100 with purified water are dissolved by heating and kept at 80 ° C to obtain an oil phase. Swell component 9 well into component 19, and then
Ingredients 1 and 11-13 are added and mixed homogeneously. Components 14 to 17 pulverized and mixed with a pulverizer are added to this, and the mixture is stirred with a homomixer and kept at 75 ° C to form an aqueous phase. The oil phase is added to this aqueous phase with stirring, cooled, and component 18 is added at 45 ° C., and the mixture is cooled to 30 ° C. with stirring to obtain a product.

[0031]   Example 7 Bath agent Prescription amount   1. Hot water extract of Indian Buderium tree (Production Example 1)                                                           5.0 copies   2. Sodium hydrogen carbonate 50.0   3. Yellow No. 202 (1) Suitable amount   4. Fragrance suitable amount   5. Adjust to 100 with sodium sulfate [Manufacturing method] Components 1 to 5 are uniformly mixed to obtain a product.

Example 8 Ointment Formulation Amount 1. Hot water extract of Indian barberry (Production Example 4) 0.01 part 2. 50% 1,3-butylene glycol extract of Indian Kino Tree (Production Example 6) 0.5 3. Polyoxyethylene cetyl ether (30 EO) 2.0 4. Glycerin monostearate 10.0 5. Liquid paraffin 5.0 6. Cetanol 6.0 7. Methyl paraoxybenzoate 0.1 8. Propylene glycol 10.0 9. [Manufacturing method] The total amount is 100 with purified water. Components 3 to 6 are dissolved by heating and mixed, and the mixture is heated to 70 ° C.
Keep it in the oil phase. Ingredients 1, 2 and 7-9 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The water phase is added to the oil phase to emulsify, and the product is cooled to 30 ° C. with stirring.

Next, in order to explain the effects of the present invention in detail, experimental examples will be given.

Experimental Example 1 Active oxygen scavenging action Using Production Examples 1, 2, 4 and 5 as samples, the scavenging action of superoxide which is a kind of active oxygen was measured. As a positive control, superoxide dismutase (SO
D) was used. In addition, in order to confirm the stability of the sample,
The sample was stored at 40 ° C. for 2 weeks and the same measurement was performed.

0.45 in 0.1 mL of the sample aqueous solution of each concentration
mL of color development reagent (0.24 mM nitroblue tetrazolium, 0.1 M phosphate buffer containing 0.4 mM xanthine; pH 8.0) and 0.45 mL of enzyme solution (0.1 U /
mL xanthine oxidase / 0.1M phosphate buffer;
pH 8.0) was added and reacted at 37 ° C. for 20 minutes to generate diformazan. Stop solution (69 mM) in this solution
After adding 1.0 mL of sodium dodecyl sulfate aqueous solution), the absorbance at a wavelength of 560 nm was measured. The inhibitory effect of each sample was calculated by the elimination rate obtained from the following formula. As a control, purified water was used instead of the sample, and 0.1 M phosphate buffer (pH 8.0) was used instead of tyrosinase as a blank. Erasure rate (%) = [1- (CD) / (AB)] × 10
0 A: Absorbance of control at 560 nm (OD 56)
0) B: O.V. D. 560 C: O.D. D. 560 D: O. of the sample blank. D. 560 The results of these tests are shown in Table 1. As a result, storage of SOD at 40 ° C. for 2 weeks significantly reduced the active oxygen scavenging activity, but extracts of Indian Buderium tree, Indian barberry and Indian quino tree did not significantly change the scavenging activity. From the above,
The extracts of Indian Buderium tree, Indian barberry and Indian quino tree showed stable and excellent active oxygen scavenging activity.

[0036]

[Table 1]

Experimental Example 2 Hyaluronidase Inhibitory Action In accordance with the method of applying the Morgan-Elson method to the hyaluronidase inhibitory action by using Production Examples 1, 2, 4 and 5 as samples [Food Hygiene Journal, 31, 3 (1990)]. Measured. That is, 175 μL of 0.1 M acetate buffer (pH 4.0) was added to the sample solution, the enzyme activity of hyaluronidase was 400 U / mL, the concentration of hyaluronic acid was 0.4 mg / mL, and the compound of the activator was 48 / mL.
80 to 0.06 mg / mL to bring the total volume to 50
After adjusting to 0 μL, the hyaluronidase reaction was carried out at 37 ° C. for 40 minutes. After the reaction, p-dimethylaminobenzaldehyde reagent was added to develop color, and the absorbance at 585 nm was measured. The inhibitory action of each sample was calculated by the inhibition rate calculated from the following formula. As a control, purified water was used instead of the sample, and as a blank, purified water was used instead of hyaluronidase. Inhibition rate (%) = [1- (CD) / (AB)] × 10
0 A: Absorbance of control at 585 nm (OD 58)
5) B: O.V. D. 585 C: O.D. D. 585 D: O. D. 585

The results of these experiments are shown in Table 2. As a result, the extracts of Indian Buderium tree, Indian barberry and Indian quino tree showed excellent hyaluronidase inhibitory activity.

[0039]

[Table 2]

Experimental Example 3 Elastase Inhibitory Action Using Production Examples 1, 2, 4 and 5 as samples, the elastase inhibitory action was measured. Using a microplate, 50 μL of 0.02 mg / mL elastase Type 3 (manufactured by Sigma) / Tris-hydrochloric acid buffer solution (pH 8.0) is added to 50 μL of the sample solution as an enzyme solution. As a substrate solution, 0.
45 mg / mL N-Succinyl-Ala-Al
a-Ala-ρ-nitroanilide (manufactured by Sigma) /0.2M Tris-HCl buffer (pH 8.0) was added to 10
After adding 0 μL and mixing, react at 37 ° C. for 1 hour, and
The absorbance at 15 nm was measured. The inhibitory action of each sample was calculated by the inhibition rate calculated from the following formula. As a control, purified water was used in place of the sample, and 0.2M Tris-HCl buffer (pH 8.0) was used in place of elastase as a blank. Inhibition rate (%) = [1- (CD) / (AB)] × 10
0 A: Absorbance of control at 415 nm (OD 41)
5) B: O.V. D. 415 C: O.D. D. 415 D: O. of the sample blank. D. 415

The results of these experiments are shown in Table 3. As a result, the extracts of Indian Buderium tree, Indian barberry and Indian quino tree showed excellent elastase inhibitory activity.

[0042]

[Table 3]

Experimental Example 4 Collagenase Inhibitory Action Using Production Examples 1, 2, 4 and 5 as samples, the collagenase inhibitory action was measured. Using a microplate, 50 μL of 0.1 mg / mL aqueous collagenase Type 4 (manufactured by Sigma) as an enzyme solution is added to 50 μL of the sample solution. As a substrate solution, 0.39 mg / mL of Pz-peptide (Pz-Pro-Leu-Gly-Pro-D-
Arg-OH, manufactured by Sigma) -Tris-hydrochloric acid buffer solution (pH 7.1) containing 20 mM calcium chloride was added and mixed to give 3
After reacting at 7 ° C. for 3 minutes, 1 mL of 25 mM citric acid was added to stop the reaction. 5 mL of ethyl acetate was added for extraction, and the ethyl acetate layer was measured for absorbance at 320 nm.
The inhibitory action of each sample was calculated by the inhibition rate calculated from the following formula. As a control, purified water was used instead of the sample, and 20 mM was used instead of collagenase as a blank.
A Tris-hydrochloric acid buffer solution (pH 7.1) containing calcium chloride was used. Inhibition rate (%) = [1- (CD) / (AB)] × 10
0 A: Absorbance of control at 320 nm (OD 32)
0) B: O.V. D. 320 C: O.D. D. 320 D: O.V. of sample blank D. 320

The results of these experiments are shown in Table 4. As a result, the extracts of Indian Buderium tree, Indian barberry and Indian quino tree showed excellent collagenase inhibitory activity.

[0045]

[Table 4]

Experimental Example 5 Use Test 1 Using the lotion of Example 1, the cream of Example 2, the conventional lotion of Comparative Example 1 and the conventional cream of Comparative Example 2, 30 women (21 to 46 years old) ) Was used for a one-month use test. After use, the effect of improving skin wrinkles and tarmi was evaluated by a questionnaire.

The results of these tests are shown in Table 5. As a result, the external preparation for skin containing the extracts of Indian Buderium tree, Indian barberry and Indian quino tree showed an excellent effect of improving wrinkles and tarmi.
During the test period, there was no skin trouble and there was no problem in safety. In addition, there was no problem with the deterioration of the prescription ingredients.

[0048]

[Table 5]

The same usage test was carried out for Examples 3 to 8 and it showed an excellent effect of improving wrinkles and tarmi.

[0050]

From the above, the extract of Indian Buderium tree, Indian barberry and Indian quino tree of the present invention has excellent active oxygen scavenging activity, hyaluronidase inhibition, elastase inhibition and collagenase inhibition activity, It was also excellent in stability. Furthermore, the external preparation for skin containing these extracts showed safe and excellent antiaging action.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61P 39/06 A61P 39/06 43/00 107 43/00 107 (72) Inventor Satoru Nakata Torimi, Nishi-ku, Nagoya-shi 2-7 Machi Nippon Menard Cosmetics Research Institute F-term (reference) 4C083 AA082 AA111 AA112 AA122 AB032 AB232 AB242 AB312 AB442 AC022 AC072 AC102 AC122 AC182 AC242 AC302 AC352 AC392 AC432 AC442 AC482 AC542 AC842 AD092 AD272 AD352 AD512 BB41 CC12 CC05 CC07 CC07 CC05 CC07 CC07 CC25 DD41 EE12 4C088 AB12 AB59 AB63 AC04 AC06 AC08 BA08 BA09 MA28 MA63 NA14 ZA89 ZC41 ZC52

Claims (3)

[Claims] 1. A skin external preparation containing one or more kinds of extracts selected from Indian Buderium tree, Indian barberry and Indian quino tree. 2. An active oxygen scavenger containing one or more extracts selected from Indian Buderium tree, Indian barberry and Indian quino tree. 3. An anti-aging agent comprising one or more kinds of extracts selected from Indian Buderium tree, Indian barberry and Indian quino tree.