JP2001524307A - ヒト椎間円板細胞の調製方法 - Google Patents

ヒト椎間円板細胞の調製方法

Info

Publication number
JP2001524307A
JP2001524307A JP2000522219A JP2000522219A JP2001524307A JP 2001524307 A JP2001524307 A JP 2001524307A JP 2000522219 A JP2000522219 A JP 2000522219A JP 2000522219 A JP2000522219 A JP 2000522219A JP 2001524307 A JP2001524307 A JP 2001524307A
Authority
JP
Japan
Prior art keywords
cells
cell
growth factor
disc
insulin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2000522219A
Other languages
English (en)
Japanese (ja)
Other versions
JP2001524307A5 (enExample
Inventor
ヘインリー，エドワード・ナサニエル，ジュニア
グルーバー，エリザベス・ヘレン
Original Assignee
シャーロット‐メクレンバーグ・ホスピタル・オーソリティ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by シャーロット‐メクレンバーグ・ホスピタル・オーソリティ filed Critical シャーロット‐メクレンバーグ・ホスピタル・オーソリティ
Publication of JP2001524307A publication Critical patent/JP2001524307A/ja
Publication of JP2001524307A5 publication Critical patent/JP2001524307A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2000522219A 1997-11-26 1998-11-24 ヒト椎間円板細胞の調製方法 Pending JP2001524307A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/979,674 US6080579A (en) 1997-11-26 1997-11-26 Method for producing human intervertebral disc cells
US08/979,674 1997-11-26
PCT/US1998/025137 WO1999027077A1 (en) 1997-11-26 1998-11-24 Method for producing human intervertebral disc cells

Publications (2)

Publication Number Publication Date
JP2001524307A true JP2001524307A (ja) 2001-12-04
JP2001524307A5 JP2001524307A5 (enExample) 2005-06-23

Family

ID=25527059

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000522219A Pending JP2001524307A (ja) 1997-11-26 1998-11-24 ヒト椎間円板細胞の調製方法

Country Status (5)

Country Link
US (2) US6080579A (enExample)
JP (1) JP2001524307A (enExample)
AU (1) AU1604599A (enExample)
CA (1) CA2311763C (enExample)
WO (1) WO1999027077A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010532994A (ja) * 2007-07-12 2010-10-21 ディスクジェニクス ヒト円板組織

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE220564T1 (de) * 1997-08-14 2002-08-15 Sulzer Innotec Ag Zusammensetzung und vorrichtung zur reparatur von knorpelgewebe in vivo bestehend aus nanokapseln mit osteoinduktiven und/oder chondroinduktiven faktoren
US7101545B1 (en) * 1997-11-26 2006-09-05 Charlotte-Mecklenburg Hospital Authority Method for using human intervertebral disc cells
US6080579A (en) * 1997-11-26 2000-06-27 Charlotte-Mecklenburg Hospital Authority Method for producing human intervertebral disc cells
ES2478257T3 (es) * 1998-10-06 2014-07-21 Stryker Corporation Composición que comprende una proteína osteogénica seleccionada del grupo que consiste en OP-2, OP-3, BMP-2, BMP-3, BMP-4, BMP-5, BMP-6, BMP-9, BMP-10, BMP-11 , BMP-15, BMP-3B, DPP, Vg-1, Vgr-1, proteína 60A, GDF-1, GDF-2, GDF-3, GDF-6, GDF-7, GDF-8, GDF-9, GDF-10, y GDF-11 para su uso en la reparación de defectos del cartílago no articular
US20020095157A1 (en) 1999-07-23 2002-07-18 Bowman Steven M. Graft fixation device combination
US6179840B1 (en) 1999-07-23 2001-01-30 Ethicon, Inc. Graft fixation device and method
US20030004574A1 (en) * 1999-10-08 2003-01-02 Ferree Bret A. Disc and annulus augmentation using biologic tissue
US6648919B2 (en) * 1999-10-14 2003-11-18 Bret A. Ferree Transplantation of engineered meniscus tissue to the intervertebral disc
JP3261456B2 (ja) * 1999-10-29 2002-03-04 独立行政法人産業技術総合研究所 細胞培養担体及び該担体を用いた細胞の培養方法
US6723335B1 (en) * 2000-04-07 2004-04-20 Jeffrey William Moehlenbruck Methods and compositions for treating intervertebral disc degeneration
AU2001270408A1 (en) * 2000-06-29 2002-01-08 Mount Sinai Hospital Intervertebral disc
US20020127265A1 (en) * 2000-12-21 2002-09-12 Bowman Steven M. Use of reinforced foam implants with enhanced integrity for soft tissue repair and regeneration
US6599323B2 (en) * 2000-12-21 2003-07-29 Ethicon, Inc. Reinforced tissue implants and methods of manufacture and use
CA2365376C (en) * 2000-12-21 2006-03-28 Ethicon, Inc. Use of reinforced foam implants with enhanced integrity for soft tissue repair and regeneration
US6852330B2 (en) 2000-12-21 2005-02-08 Depuy Mitek, Inc. Reinforced foam implants with enhanced integrity for soft tissue repair and regeneration
US7008433B2 (en) * 2001-02-15 2006-03-07 Depuy Acromed, Inc. Vertebroplasty injection device
EP1660001B1 (en) * 2001-08-31 2019-10-09 Orthopeutics, LP Crosslinking reagent for treating vertebral disc disorders
KR100944204B1 (ko) * 2001-09-15 2010-02-26 러쉬 유니버시티 메디컬 센터 중층 연골 조직 및 그것을 조작하는 방법
US20030165473A1 (en) * 2001-11-09 2003-09-04 Rush-Presbyterian-St. Luke's Medical Center Engineered intervertebral disc tissue
US7232802B2 (en) 2001-12-21 2007-06-19 Zimmer Orthobiologics, Inc. Compositions and methods for promoting myocardial and peripheral angiogenesis
US20030138873A1 (en) * 2002-01-22 2003-07-24 Koichi Masuda Tissue engineered cartilage for drug discovery
AU2002235518A1 (en) * 2002-02-04 2003-09-02 Bret A. Ferree Treating degenerative disc disease through transplantation of allograft disc
US7622562B2 (en) 2002-06-26 2009-11-24 Zimmer Orthobiologics, Inc. Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
US7744651B2 (en) * 2002-09-18 2010-06-29 Warsaw Orthopedic, Inc Compositions and methods for treating intervertebral discs with collagen-based materials
US20040054414A1 (en) 2002-09-18 2004-03-18 Trieu Hai H. Collagen-based materials and methods for augmenting intervertebral discs
US20040078090A1 (en) 2002-10-18 2004-04-22 Francois Binette Biocompatible scaffolds with tissue fragments
US7824701B2 (en) * 2002-10-18 2010-11-02 Ethicon, Inc. Biocompatible scaffold for ligament or tendon repair
CN100394989C (zh) * 2002-11-15 2008-06-18 华沙整形外科股份有限公司 包含微粒状基于胶原材料的组合物的制药应用和包含所述组合物的滑膜关节
US20040186471A1 (en) * 2002-12-07 2004-09-23 Sdgi Holdings, Inc. Method and apparatus for intervertebral disc expansion
WO2004069296A1 (en) * 2003-01-31 2004-08-19 Zimmer Orthobiologics Inc. Hydrogel compositions comprising nucleus pulposus tissue
US20060015182A1 (en) * 2003-02-25 2006-01-19 Tsou Paul M Patch material for intervertebral disc annulus defect repair
US8197837B2 (en) 2003-03-07 2012-06-12 Depuy Mitek, Inc. Method of preparation of bioabsorbable porous reinforced tissue implants and implants thereof
ATE488205T1 (de) 2003-03-14 2010-12-15 Depuy Spine Inc Hydraulische vorrichtung zur knochenzementeinspritzung bei perkutaner vertebroplastie
US8066713B2 (en) 2003-03-31 2011-11-29 Depuy Spine, Inc. Remotely-activated vertebroplasty injection device
US8415407B2 (en) 2004-03-21 2013-04-09 Depuy Spine, Inc. Methods, materials, and apparatus for treating bone and other tissue
US8226715B2 (en) 2003-06-30 2012-07-24 Depuy Mitek, Inc. Scaffold for connective tissue repair
US10583220B2 (en) 2003-08-11 2020-03-10 DePuy Synthes Products, Inc. Method and apparatus for resurfacing an articular surface
WO2005030034A2 (en) 2003-09-26 2005-04-07 Depuy Spine, Inc. Device for delivering viscous material
US7316822B2 (en) 2003-11-26 2008-01-08 Ethicon, Inc. Conformable tissue repair implant capable of injection delivery
US7901461B2 (en) 2003-12-05 2011-03-08 Ethicon, Inc. Viable tissue repair implants and methods of use
US7824711B2 (en) 2003-12-11 2010-11-02 Isto Technologies, Inc. Particulate cartilage system
WO2005055877A2 (de) * 2003-12-12 2005-06-23 Co.Don Ag Verfahren zur herstellung von bandscheibengewebe aus degeneriertem bandscheibengewebe
US11395865B2 (en) * 2004-02-09 2022-07-26 DePuy Synthes Products, Inc. Scaffolds with viable tissue
US8657881B2 (en) * 2004-04-20 2014-02-25 Depuy Mitek, Llc Meniscal repair scaffold
US8137686B2 (en) 2004-04-20 2012-03-20 Depuy Mitek, Inc. Nonwoven tissue scaffold
US8221780B2 (en) 2004-04-20 2012-07-17 Depuy Mitek, Inc. Nonwoven tissue scaffold
EP1614402A1 (en) * 2004-07-09 2006-01-11 Paul M. Tsou Patch material for inervertebral disc annulus defect repair
CN106963464B (zh) 2004-07-30 2019-11-05 德普伊新特斯产品有限责任公司 外科用具
US8697139B2 (en) 2004-09-21 2014-04-15 Frank M. Phillips Method of intervertebral disc treatment using articular chondrocyte cells
US9381024B2 (en) 2005-07-31 2016-07-05 DePuy Synthes Products, Inc. Marked tools
US9918767B2 (en) 2005-08-01 2018-03-20 DePuy Synthes Products, Inc. Temperature control system
EP1916964A4 (en) 2005-08-26 2015-11-04 Zimmer Inc IMPLANTS AND METHODS FOR THE REPAIR, REPLACEMENT AND TREATMENT OF JOINT DISEASES
US8360629B2 (en) 2005-11-22 2013-01-29 Depuy Spine, Inc. Mixing apparatus having central and planetary mixing elements
WO2007136673A2 (en) * 2006-05-19 2007-11-29 Medistem Laboratories, Inc. Treatment of disc degenerative disease and compositions for same
US20080004703A1 (en) * 2006-06-30 2008-01-03 Warsaw Orthopedic, Inc. Method of treating a patient using a collagen material
US8399619B2 (en) * 2006-06-30 2013-03-19 Warsaw Orthopedic, Inc. Injectable collagen material
US8118779B2 (en) * 2006-06-30 2012-02-21 Warsaw Orthopedic, Inc. Collagen delivery device
US20080004431A1 (en) * 2006-06-30 2008-01-03 Warsaw Orthopedic Inc Method of manufacturing an injectable collagen material
US8048618B2 (en) * 2006-07-26 2011-11-01 The University Of Hong Kong Method for preparing and preserving a natural allograft intervertebral disk for humans
AU2007297097A1 (en) 2006-09-14 2008-03-20 Depuy Spine, Inc. Bone cement and methods of use thereof
CA2747850C (en) 2006-10-19 2013-05-14 Depuy Spine, Inc. Fluid delivery system
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
US9827023B2 (en) 2007-01-29 2017-11-28 Life Spine, Inc. Craniospinal fusion method and apparatus
US8043342B2 (en) * 2007-01-29 2011-10-25 Polaris Biotechnology, Inc. Craniospinal fusion method and apparatus
US8403965B2 (en) * 2007-01-29 2013-03-26 Polaris Biotechnology, Inc. Vertebra attachment method and system
US8556939B2 (en) * 2008-01-08 2013-10-15 Fraser Cummins Henderson Mathematical relationship of strain, neurological dysfunction and abnormal behavior resulting from neurological dysfunction of the brainstem
US8182511B2 (en) * 2007-01-29 2012-05-22 Polaris Biotechnology, Inc. Craniospinal fusion method and apparatus
US20090036894A1 (en) * 2007-01-29 2009-02-05 Polaris Biotechnology, Inc. Method of treating a neurological condition through correction and stabilization of the clivo-axial angle
AU2008240191B2 (en) 2007-04-12 2013-09-19 Zimmer, Inc. Compositions and methods for tissue repair
WO2009089395A2 (en) * 2008-01-08 2009-07-16 Polaris Biotechnology, Inc. Osteointegration apparatus
WO2010027789A2 (en) 2008-08-25 2010-03-11 University Of South Florida Volume exclusion agent to enhance formation of extracelluar matrix
KR20220100999A (ko) * 2008-11-25 2022-07-18 코오롱 티슈진 인크. 감작 세포 치료요법
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
US11285177B2 (en) 2018-01-03 2022-03-29 Globus Medical, Inc. Allografts containing viable cells and methods thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4485097A (en) * 1982-05-26 1984-11-27 Massachusetts Institute Of Technology Bone-equivalent and method for preparation thereof
US5266476A (en) * 1985-06-18 1993-11-30 Yeda Research & Development Co., Ltd. Fibrous matrix for in vitro cell cultivation
US5032508A (en) * 1988-09-08 1991-07-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5153132A (en) * 1988-06-30 1992-10-06 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Three-dimensional co-culture process
US5496722A (en) * 1988-06-30 1996-03-05 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Method for producing non-neoplastic, three dimensional, mammalian tissue and cell aggregates under microgravity culture conditions and the products produced therefrom
US5422340A (en) * 1989-09-01 1995-06-06 Ammann; Arthur J. TGF-βformulation for inducing bone growth
FR2654625B1 (fr) * 1989-11-22 1992-02-21 Transphyto Sa Procede de fabrication d'un materiau pour osteoplastie a partir d'un tissu osseux naturel et materiau obtenu par ce procede.
US5256418A (en) * 1990-04-06 1993-10-26 Organogenesis, Inc. Collagen constructs
US5964807A (en) * 1996-08-08 1999-10-12 Trustees Of The University Of Pennsylvania Compositions and methods for intervertebral disc reformation
WO1999004720A1 (en) * 1997-07-11 1999-02-04 Reprogenesis Inc. Repair of intervertebral disks
US6080579A (en) 1997-11-26 2000-06-27 Charlotte-Mecklenburg Hospital Authority Method for producing human intervertebral disc cells
US7101545B1 (en) * 1997-11-26 2006-09-05 Charlotte-Mecklenburg Hospital Authority Method for using human intervertebral disc cells
US6340369B1 (en) 1999-08-13 2002-01-22 Bret A. Ferree Treating degenerative disc disease with harvested disc cells and analogues of the extracellular matrix

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010532994A (ja) * 2007-07-12 2010-10-21 ディスクジェニクス ヒト円板組織
US9487753B2 (en) 2007-07-12 2016-11-08 Discgenics Compositions of adult disc stem cells and methods for the treatment of degenerative disc disease
US11168305B2 (en) 2007-07-12 2021-11-09 Discgenics, Inc. Methods for the treatment of degenerative disc disease

Also Published As

Publication number Publication date
AU1604599A (en) 1999-06-15
US7445776B2 (en) 2008-11-04
WO1999027077A1 (en) 1999-06-03
CA2311763C (en) 2014-01-21
US6080579A (en) 2000-06-27
CA2311763A1 (en) 1999-06-03
US20050232903A1 (en) 2005-10-20

Similar Documents

Publication Publication Date Title
JP2001524307A (ja) ヒト椎間円板細胞の調製方法
Gruber et al. Human intervertebral disc cells from the annulus: three-dimensional culture in agarose or alginate and responsiveness to TGF-β1
JP3808900B2 (ja) 結合組織細胞に部分的又は完全に分化した骨髄幹細胞の有効培養物及びヒアルロン酸誘導体より成る三次元の生体親和性で且つ生分解性のマトリックスから構成される生物学的物質
RU2272839C2 (ru) Бессывороточная среда для культуры клеток, используемых для реконструкции костных и хрящевых сегментов (варианты)
US6761887B1 (en) Alginate layer system for chondrogenic differentiation of human mesenchymal stem cells
DE69233029T2 (de) Menschliche mesenchym-stammzellen aus dem mark und monoklonale antikörper spezifisch gegen diese zellen
US6197586B1 (en) Chondrocyte-like cells useful for tissue engineering and methods
Jaffe et al. The neuroectodermal tumor of bone
EP0339607B1 (en) Composition for repair of cartilage and bone and method for their preparation as skeletal tissue implant
US20040077079A1 (en) Improved in vitro method of culturing mammalian cells for autologous cell implantation/transplantation methods
Redlich et al. Bone engineering on the basis of periosteal cells cultured in polymer fleeces
US20120219533A1 (en) Method for the production of intervertebral disk cell transplants and their use as transplantation material
JP2003534792A (ja) 軟骨代替物の培養方法及びその方法により製造されるバイオマトリックス
US20100247601A1 (en) Repair and treatment of bone defect using cells induced by agent produced by chondrocytes capable of hypertrophication and scaffold
US20080187518A1 (en) Production of Osteoclasts from Adipose Tissues
US7101545B1 (en) Method for using human intervertebral disc cells
JP2004254655A (ja) 軟骨の分化誘導方法
US20050142660A1 (en) Conditioned medium for culturing Schwann cells
Fukui et al. Regional Differences in Chondrocyte Metabolism in
AU2002227888A1 (en) An improved in vitro method of culturing mammalian cells for autologous cell implantation/transplantation methods
HK1133035A (en) Methods to maintain, improve and restore the cartilage phenotype of chondrocytes
MXPA97005612A (en) Induction directed by lineage of the differentiation of human mesenquimatosas cells of ori

Legal Events

Date Code Title Description
A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20041220

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20050118

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20050614