JP2001524307A5 - - Google Patents

Info

Publication number

JP2001524307A5

JP2001524307A5 JP2000522219A JP2000522219A JP2001524307A5 JP 2001524307 A5 JP2001524307 A5 JP 2001524307A5 JP 2000522219 A JP2000522219 A JP 2000522219A JP 2000522219 A JP2000522219 A JP 2000522219A JP 2001524307 A5 JP2001524307 A5 JP 2001524307A5

Authority

JP

Japan

Prior art keywords

intervertebral disc

human intervertebral

cells

monolayer

minced

Prior art date

1998-11-24

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims 11
• 238000012258 culturing Methods 0.000 claims 5
• 239000002356 single layer Substances 0.000 claims 5
• 239000013553 cell monolayer Substances 0.000 claims 4
• 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 2
• 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 2
• 238000004113 cell culture Methods 0.000 claims 2
• 238000000338 in vitro Methods 0.000 claims 2
• 230000000644 propagated effect Effects 0.000 claims 2
• ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims 2
• FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical group O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims 1
• 229920000936 Agarose Polymers 0.000 claims 1
• 108091003079 Bovine Serum Albumin Proteins 0.000 claims 1
• 102000004127 Cytokines Human genes 0.000 claims 1
• 108090000695 Cytokines Proteins 0.000 claims 1
• 229940072056 alginate Drugs 0.000 claims 1
• 235000010443 alginic acid Nutrition 0.000 claims 1
• 229920000615 alginic acid Polymers 0.000 claims 1
• 239000012894 fetal calf serum Substances 0.000 claims 1
• 239000003102 growth factor Substances 0.000 claims 1
• 238000010899 nucleation Methods 0.000 claims 1
• 210000002966 serum Anatomy 0.000 claims 1