JP2001286550A - Blood component sampler - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a blood component sampler that can sample desirable platelet-including plasma with fewer leukocytes and erythrocytes mixed, even from a donor with a low hematocrit value and one with a low sampling blood flow. SOLUTION: The blood component sampler 1 comprises a centrifugal separator 20, a turbidity sensor 14 fixed around the outlet of the centrifugal separator, and a control part 13. The control part has a platelet sampling process pattern determining function of using, in a platelet sampling step, a signal detected by the turbidity sensor and a signal change pattern computed from the signal to thus determine which pattern the platelet sampling process follows out of a normal pattern and a plurality of exceptional patters stored, and a platelet sampling step ending time controlling function of ending the platelet sampling step at timing suitable for the pattern determined by the platelet sampling process pattern determining function.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood component collecting apparatus for separating a predetermined blood component from blood.

[0002]

2. Description of the Related Art At the time of blood collection, currently, blood is separated into each blood component by centrifugation or the like, for reasons such as effective use of blood and reduction of the burden on donors, and only components necessary for the transfuser are collected. Blood is collected from components collected and returned to donors. In such a component blood sampling, when a platelet preparation is obtained, blood collected from a donor is introduced into a blood component collection circuit, and a plasma, a centrifugal bowl called a centrifugal bowl installed in the blood component collection circuit is used.
White blood cells, platelets and erythrocytes are separated into four components, platelets among them are collected in a container to obtain a platelet product, and plasma is collected in a container as a raw material of a plasma product or a plasma fraction product,
The remaining white blood cells and red blood cells are returned to the donor.

As a method for collecting platelets, for example, Japanese Patent Application Laid-Open
There is a method disclosed in JP-A-509403. Japanese Patent Publication No. Hei 8-509403 discloses a first method in which a liquid is added to whole blood at a predetermined flow rate and diluted and sent to a centrifugal bowl. Whole blood is sent to a centrifugal bowl, and centrifuged to separate low-density components. After separating the medium density component and the high density component into the first container and taking out the low density component into the first container, the circuit is switched and the low density component is circulated at the first flow rate (constant speed) so as to be circulated in the centrifugal bowl. A second method is disclosed in which a density component region is expanded and a medium density component is extracted while recirculating at a second flow rate (acceleration). In this blood component collection apparatus, the end timing of platelet collection is performed using a change in a signal detected by a line sensor (turbidity sensor) attached to a line near the outlet of the centrifuge. In the normal state, the change pattern of the signal detected by the turbidity sensor at the time of platelet collection falls sharply from the turbidity sensor reference value and rises again from the inflection point (lower limit peak) as shown in FIG. Have an inflection point again at a value lower than the reference value. The blood component collecting apparatus disclosed in Japanese Patent Publication No. Hei 8-509403 utilizes this pattern to determine the end timing of platelet collection. However, when the present inventor examined many blood collection examples, it was found that the change pattern of the signal detected by the turbidity sensor includes not only the normal pattern as described above but also a plurality of exceptional patterns. This is considered to be caused by variations in the blood properties (hematocrit value) of the donor or variations in the blood flow state of the donor. In the case of such an exceptional pattern, if the end timing of platelet collection is determined based on the assumption of a normal pattern, if the target number of platelets to be collected is not reached, or if white blood cells that do not want to collect more platelets at the same time And the case where even red blood cells are collected.

[0004]

SUMMARY OF THE INVENTION A blood component collection apparatus capable of collecting a good platelet-containing plasma with little white blood cells and red blood cells even in a donor having a low hematocrit value or a donor having a low blood collection flow rate. I will provide a.

[0005]

Means for achieving the above object is to separate blood into a plurality of blood components by a centrifuge having a blood storage space therein, and to collect a specific blood component into a bag. A blood collection device, comprising: a first line for connecting blood collection means and an inlet of the centrifuge; and a second line connected to an outlet of the centrifuge. A plasma collection bag having a first tube connected in the middle of the first line and a second tube connected to the second line;
A blood component collection circuit consisting of a platelet collection bag connected to the line, a turbidity sensor attached to the outlet of the centrifuge or the second line, and detection by the turbidity sensor when collecting platelets A platelet collection process pattern determining function of determining whether the platelet collection process is one of a stored normal pattern or a plurality of exceptional patterns using the signal to be performed and the signal change pattern calculated from the signal, At a time suitable for the pattern determined by the platelet collection process pattern determination function,
This is a blood component collecting apparatus having an adjustment function at the end of the platelet collection step for terminating the platelet collection step.

In the platelet collection step, the platelet collection process pattern judgment function utilizes a signal detected by the turbidity sensor, a signal change pattern calculated from the signal, and a circulation speed at the time of platelet collection. Preferably, the platelet collection process is to determine whether the stored normal pattern or a plurality of exceptional patterns are stored. In addition, the blood component collection apparatus, when the platelet collection process pattern determination function determines that any one of a plurality of exceptional patterns, the exception that the platelet collection conditions are determined in the next platelet collection step It is preferable to have a platelet collection condition adjusting function for adjusting the platelet collection conditions to conditions suitable for the pattern. Further, the platelet collection condition adjusting function may adjust the platelet collection condition such that the change pattern of the signal detected by the turbidity sensor at the time of the platelet collection step approaches the stored normal pattern. preferable.

[0007] The blood component collecting apparatus may further include a centrifugal separator driving device for rotating a rotor of the centrifugal separator, and a centrifugal separator side with respect to a connection between the first line and the first tube. A first liquid pump for the first line and a second liquid pump for the third line
Pump, a plurality of flow path opening / closing means for opening and closing the flow path of the blood component collection circuit, the centrifugal separator driving device, the first liquid pump, and the second liquid pump And a control unit for controlling the plurality of flow path opening / closing means, the control unit collects blood to which an anticoagulant is added,
Separating collected blood and collecting the separated plasma into the plasma collection bag, and plasma circulating the plasma in the plasma collection bag collected by the plasma collection step to the centrifuge At least one plasma collection / circulation step comprising the steps of: (a) and (c), after the plasma collection / circulation step is completed, accelerating the plasma circulation speed by the first liquid sending pump to cause the platelets to flow out of the centrifuge. A platelet collection step of causing the platelet collection bag to collect the platelets in the platelet collection bag, and performing a blood return step of returning blood in the centrifuge after the platelet collection step is completed. , The centrifuge drive device, the first liquid feed pump,
It is preferable that the control means controls the second liquid feed pump and the plurality of flow path opening / closing means, and has the function of determining the pattern of platelet collection process and the function of adjusting the end of platelet collection.

[0008] The control unit may control the centrifugal separator driving device, the first liquid feed pump, the second liquid feed pump, and the plurality of flows so that the platelet collection operation is performed at least twice. It is preferable to control the road opening / closing means. Further, the control unit may be configured to perform the first liquid supply pump and the second liquid supply after the plasma collection / circulation step is completed and before the plasma circulation speed by the first liquid supply pump is accelerated. Activating a pump to collect blood to which an anticoagulant has been added, and activating the centrifugal separator driving device to perform a plasma collection step of collecting a predetermined amount of plasma from the blood into the plasma collection bag. It is preferred that Further, the control unit controls the plasma collection / circulation step to be performed twice. In the first plasma collection / circulation step, the plasma in the plasma collection bag is sent to the centrifugal separator at a constant speed. A plasma collection / constant speed circulation step for circulating is performed. In a second plasma collection / circulation step, a plasma collection / accelerated circulation step for circulating the plasma in the plasma collection bag while accelerating the plasma in the centrifugal separator is performed. Preferably, it is controlled to be performed.

[0009] The blood component collection circuit may include a second component.
Buffy coat collection bag connected to the line, the control unit, after the end of the platelet collection step, before the blood return step, the buffy coat outflow from the centrifuge, the buffy coat It is preferable to perform a buffy coat collection step of collecting the buffy coat in a buffy coat collection bag. Further, when the platelet collection operation is further performed after the completion of the buffy coat collection step, the control unit may return the collected buffy coat to the centrifuge before the next plasma collection / circulation step. It is preferable that the first liquid feed pump and the plurality of flow path opening / closing means are controlled so that the coat return step is performed.

[0010] The platelet collecting apparatus includes: a step of collecting blood to which an anticoagulant is added, a step of collecting the separated blood, and a step of collecting the separated plasma in the plasma collection bag; A plasma circulation step of circulating the plasma in the plasma collection bag collected by the step to the centrifugal separator, at least one plasma collection / circulation step; and, after the plasma collection / circulation step, A platelet collecting step of accelerating the plasma circulation speed of the liquid sending pump to allow the platelets to flow out of the centrifugal separator and collect the platelets in the platelet collection bag. It has a function to control the platelet collection operation to be performed at least twice so as to perform a blood return step of returning blood in the vessel. Preferred.

The platelet collecting process pattern judging function uses the signal detected by the turbidity sensor, a signal change pattern calculated from the signal, and a circulation speed at the time of platelet collecting in the platelet collecting step. Determining whether the platelet collection process is a stored normal pattern or a plurality of exceptional patterns, and further, the plasma collection device determines that the first platelet collection process is a normal pattern. A first platelet collection process data storage unit that stores data relating to the first platelet collection process, and a data comparison function for comparing data in the platelet collection process in the next and subsequent platelet collection steps with the first platelet collection process data. Wherein the adjustment function at the end of the platelet collection step includes the data ratio Function and preferably has a function to terminate the platelet collection step, in case of discrepancies over predetermined between compared platelet collection process the data with the first platelet collection step data is detected by. Further, it is preferable that the blood component collection device has a warning function that is activated when a difference of not less than a predetermined value is detected between the initial platelet collection process data and the comparison target platelet collection process data by the data comparison function.

The control unit measures and stores the peak time from the start of the platelet collection step to the detection of the lower limit peak voltage by the turbidity sensor during the first platelet collection operation, and stores the lower limit peak voltage. A comparison function for comparing whether the difference between the turbidity sensor voltage and the lower limit peak voltage at the time of the peak time from the start of the platelet collection step during the second and subsequent platelet collection operations is greater than a predetermined value, The adjustment function at the end of the platelet collection step, the difference between the turbidity sensor voltage and the lower limit peak voltage when the peak time has been reached from the start of the platelet collection step in the second and subsequent platelet collection operations by the comparison function. When it is determined that the turbidity sensor voltage change rate in a predetermined second is larger than a predetermined value, Calculated, and it is preferable calculated voltage change rate is what end the platelet collection step is smaller than a predetermined value. Further, the adjustment function at the end of the platelet collection step of the control unit is configured such that, when the voltage change rate in the predetermined second calculated by the voltage change rate calculation function is larger than the predetermined value, the voltage change rate calculation function again performs the predetermined time. To calculate the second rate of voltage change at
It is preferable that the platelet collection step be ended when the calculated second voltage change rate is smaller than a predetermined value.

Another object of the present invention is to provide a blood component collecting apparatus for separating blood into a plurality of blood components by a centrifugal separator having a blood storage space therein and collecting a specific blood component into a bag. Wherein the blood component collection device comprises: a first line for connecting blood collection means and an inlet of the centrifuge; a second line connected to an outlet of the centrifuge; Blood comprising a plasma collection bag having a first tube connected in the middle of the first line and a second tube connected to the second line, and a platelet collection bag connected to the second line A component collection circuit, an outlet of the centrifuge or the second
A turbidity sensor attached to the line, a centrifuge driving device and a control unit for rotating the rotor of the centrifuge, and the control unit collects and collects blood to which an anticoagulant is added. A plasma collection step of separating the separated blood and collecting the separated plasma in the plasma collection bag;
At least one plasma collection / circulation step including a plasma circulation step of circulating the plasma in the plasma collection bag collected by the plasma collection step to the centrifuge, and after the plasma collection / circulation step, The first
A platelet collection step of accelerating the plasma circulation speed of the liquid sending pump to allow the platelets to flow out of the centrifugal separator and collect the platelets in the platelet collection bag. Controlling the platelet collection operation to perform a blood return step of returning the blood in the vessel to be performed at least twice, and further, the control unit controls the platelet collection operation during the first platelet collection operation The measurement and storage of the peak time from the start of the step to the detection of the lower limit peak by the turbidity sensor, and the storage function of the lower limit peak voltage, and the time from the start of the platelet collection step to the time of the peak time at the time of the second and subsequent platelet collection operations A comparison function for comparing whether the difference between the turbidity sensor voltage and the lower limit peak voltage is larger than a predetermined value, When it is determined by the comparison function that the difference between the turbidity sensor voltage and the lower limit peak voltage at the time of reaching the peak time from the start of the platelet collection step during the second and subsequent platelet collection operations is greater than a predetermined value A voltage change rate calculation function for calculating a voltage change rate of the turbidity sensor for a predetermined second; and a platelet collection step for terminating the platelet collection step when the voltage change rate calculated by the voltage change rate calculation function is smaller than a predetermined value. This is a blood component sampling device having a time adjustment function. When the voltage change rate in the predetermined time period calculated by the voltage change rate calculation function is larger than the predetermined value, the adjustment function at the end of the platelet collection step of the control unit is performed again by the voltage change rate calculation function for a predetermined time period. It is preferable that the platelet collection step is terminated when the calculated second voltage change rate is smaller than a predetermined value.

[0014]

DESCRIPTION OF THE PREFERRED EMBODIMENTS A blood component collecting apparatus according to the present invention will be described with reference to an embodiment shown in the drawings. FIG. 1 is a plan view showing a configuration example of a blood component collection circuit used in the blood component collection device of the present invention. FIG. 2 is a plan view of a cassette housing portion of the blood component collection circuit of FIG. FIG. 3 is a partially cutaway sectional view showing a state in which a driving device is mounted on a centrifuge used in a blood component collection circuit, and FIG. It is a conceptual diagram of one Example of an apparatus. The blood component collection device 1 of the present invention is a blood component collection device for separating blood into a plurality of blood components by a centrifugal separator 20 having a blood storage space therein and collecting a specific blood component into a bag. The blood component collection device 1 includes a first line 21 for connecting a blood collection unit (connection part to the blood collection needle 29 or the blood pool) and an inlet of the centrifuge 20, and an outlet of the centrifuge 20. Collection bag 2 having a second line 22 connected to the first line, a first tube 25a connected to the middle of the first line, and a second tube 25b connected to the second line.
5 and a platelet collection bag 26 connected to the second line
Is attached. The blood component collection device 1 includes a turbidity sensor 14 attached to an outlet or a second line of the centrifuge 20, a signal detected by the turbidity sensor when collecting platelets, and a signal change calculated from the signal. Uses the pattern to determine whether the platelet collection process is one of the stored normal pattern or multiple exceptional patterns, and is suitable for the pattern determined by the platelet collection process pattern determination function. At the point in time, a platelet collection step end adjustment function for terminating the platelet collection step is provided.

The blood component collection device 1 includes a rotor 142 having a blood storage space therein, and an inlet 1 communicating with the blood storage space.
A centrifugal separator 20 having a centrifugal separator 43 having an inlet 43 and an outlet 144 for centrifuging the blood introduced from the inlet 143 in the blood storage space by the rotation of the rotor 142; a connection to the blood collection needle 29 or the blood pool; Inlet 143 of vessel 20
And a first line 21 for connecting the
A second line 22 connected to the outlet 144 of the first anti-coagulant and a third line connected to the first line 21 for anticoagulant injection.
, A first tube 25a connected to the first line 21 and a second tube connected to the second line 22.
The blood component collection device for the blood component collection circuit 2 includes a plasma collection bag 25 having a tube 25b and a platelet collection bag 26 connected to the second line 22. The blood component collection device 1 includes a centrifuge drive device 10 for rotating the rotor 142 of the centrifuge 20,
A first liquid supply pump 11 for the first line 21, a second liquid supply pump 12 for the third line 23, and a plurality of ports for opening and closing the flow path of the blood component collection circuit 2. Flow path opening / closing means 81, 82, 83, 84, 85, 86, 87;
Centrifuge drive 10, first liquid feed pump 11, second
And a control unit 13 for controlling the liquid sending pump 12 and the plurality of flow path opening / closing means. This blood component collection device
Since the apparatus is constituted by two pumps, the size of the apparatus can be reduced.

Therefore, the blood component collecting circuit 2 will be described first. The blood component collection circuit 2 is a circuit for collecting blood components, especially platelets. The platelet collection circuit 2 includes a blood collection device such as a blood collection needle 29, a connection portion to a blood collection device having a blood collection needle or a blood pool connection portion (blood collection device connection portion), a blood collection needle 29 or a blood collection device connection portion, and a centrifuge. 20, a first line 21 (blood collection and blood return line) including a first pump tube 21g, an outlet 144 of the centrifuge 20, and a first line 21.
A second line 22 for connecting the first line 21 to the blood collection needle 29 of the first line 21 and a third line 23 provided with a second pump tube 23a (an anticoagulant injection line). The first tube 25a and the second line 22 connected to the branch connector 21f located on the blood collection needle side of the pump tube 21g of the first line 21.
Collection bag 25 having a second tube 25b connected to the third tube 2 connected to the second line 22
Platelet collection bag 26 with 6a, second line 22
And a fourth line 24 for injecting a liquid (saline) connected to the second line 22. The blood component collection circuit 2 is not a blood collection needle, but a connection unit for connecting to a blood pool such as a blood bag (for example,
(A metal or synthetic resin needle).

As the blood collection needle 29, a known metal needle is used. The first line 21 is connected to the blood collection needle side first line 21 a to which the blood collection needle 29 is connected and the inflow port 14 of the centrifuge 20.
3 and the first line 21b connected to the centrifugal separator. The first line 21a on the blood collection needle side is formed by connecting a plurality of soft resin tubes. The blood collection needle side first line 21a includes, from the blood collection needle side, a branch connector 21c for connection to the third line 23, a chamber 21d for removing bubbles and microaggregates, and a second line 22.
Branch connector 21e for connection with the plasma collection bag 25
Branch connector 21f for connection with the first tube 25a
Is provided. An air-permeable and bacteria-impermeable filter 21i is connected to the chamber 21d. The centrifuge-side first line 21b is connected to a branch connector 21f for connection to the first tube 25a, and has a pump tube 21g formed in the vicinity thereof.

The second line 22 connecting the outlet 144 of the centrifuge 20 and the first line 21 has one end connected to the outlet 144 of the centrifuge 20 and the other end connected to the first line 21. Is connected to the connection branch connector 21e. The second line 22 is a branch connector 22a for connection with the second tube 25b of the plasma collection bag 25 and the third tube 26a of the platelet collection bag 26, and a connection branch for connection with the fourth line 24 from the centrifuge side. The baffle coat collecting bag 27 includes a connector 22b, a branch connector 22c for connection to a tube including a bubble removal filter 22f, and a branch connector 22d for connection to a fourth tube 27a of the buffy coat collection bag 27. One end of the third line 23 is the first line.
Connection branch connector 21c provided on line 21
It is connected to the. The third line 23 is the connector 2
From the side 1c, a pump tube 23a, a filter 23b for removing foreign matter, a chamber 23c for removing bubbles, and a needle 23d for connecting an anticoagulant container are provided. The fourth line 24 is
One end is a branch connector 22b for connection of the second line 22
It is connected to the. The fourth line 24 is the connector 2
From the side 2b, a filter 24a for removing foreign matter and a needle 24b for connecting a physiological saline container are provided.

The plasma collection bag 25 is connected to the first line 21
The first tube 25a and the second tube 25a connected to the branch connector 21f located closer to the blood collection needle than the pump tube 21g
Connected to the branch connector 22a of the line 22 of FIG.
It has a tube 25b. The platelet collection bag 26 includes a third tube 26a connected to the branch connector 22a of the second line 22. The buffy coat collection bag 27 includes a fourth tube 27a connected to the branch connector 22d of the second line 22. The constituent materials of the tubes used for forming the first to fourth lines 21, 22, 23, 24, the pump tubes, and the tubes connected to the bag include, for example, polyvinyl chloride, polyethylene, Polypropylene, polyesters such as PET and PBT, ethylene-vinyl acetate copolymers, polyurethanes, polyester elastomers, thermoplastic elastomers such as styrene-butadiene-styrene copolymers and the like, among which polyvinyl chloride is particularly preferred. preferable. If each tube is made of polyvinyl chloride, sufficient flexibility and flexibility can be obtained, so that it is easy to handle, and it is also suitable for clogging with clamps or the like. Also, as the constituent material of the branch connector described above, the same material as the constituent material of the tube can be used. As the pump tube, a tube having such a strength as not to be damaged even when pressed by a roller pump is used.

Plasma collection bag 25, platelet collection bag 2
6. The buffy coat collection bag 27 is formed by laminating a resin-made flexible sheet material and fusing (thermal fusion, high-frequency fusion, or the like) or bonding the periphery thereof to form a bag. used. As a material used for each of the bags 25, 26, 27, for example, soft polyvinyl chloride is preferably used. As a plasticizer in the soft polyvinyl chloride, for example, di (ethylhexyl) phthalate (D
EHP), di- (n-decyl) phthalate (DnDP)
Etc. are used. Incidentally, the content of such a plasticizer,
The amount is preferably about 30 to 70 parts by weight based on 100 parts by weight of polyvinyl chloride. In addition, each of the bags 25, 2
As the sheet material 6, 27, a polyolefin, that is, a polymer obtained by polymerizing or copolymerizing an olefin such as ethylene, propylene, butadiene, or isoprene or a diolefin may be used. Specifically, for example, polyethylene, polypropylene, ethylene-vinyl acetate copolymer (EVA), a polymer blend of EVA and various thermoplastic elastomers, or a combination thereof arbitrarily. Further, polyesters such as polyethylene terephthalate (PET), polybutylene terephthalate (PBT), and poly-1,4-cyclohexanedimethyl terephthalate (PCHT), and polyvinylidene chloride can also be used.

As the sheet material used in the platelet collection bag 26, it is more preferable to use a sheet material having excellent gas permeability in order to improve the platelet preservability. As such a sheet material, for example, using the above-described polyolefin or DnDP plasticized polyvinyl chloride,
Further, without using such a material, a sheet material of the above-described material is used, and the thickness is relatively thin (for example,
Those having a thickness of about 0.1 to 0.5 mm, particularly about 0.1 to 0.3 mm) are preferable. Further, the platelet collection bag includes, for example, physiological saline, GAC, PAS, PSM-1.
Such a platelet preservation solution as described above may be stored in advance.

The main part of the blood component collecting circuit 2 is of a cassette type as shown in FIG. The blood component collection circuit 2 includes all lines (first line, second line, third line, and fourth line) and all tubes (first tube, second tube, third tube, and fourth line). Tubes) are partially housed and partially hold them, in other words comprise a cassette housing 28 in which they are partially fixed. Both ends of a first pump tube 21g and both ends of a second pump tube 23a are fixed to the cassette housing 28. The pump tubes 21g and 23a are formed into a loop shape corresponding to the shape of the roller pump by the cassette housing 28. It is protruding. Therefore, the first and second pump tubes 21g and 23a can be easily mounted on the roller pump.

Further, the cassette housing 28 has a plurality of openings located in the cassette housing 28. Specifically, the first opening 91 exposing the first line 21 on the blood collection needle side from the pump tube 21g and allowing the first flow path opening / closing means 81 of the blood component collection device 1 to enter, First tube 25a of plasma collection bag 25
And the second opening 92 through which the second channel opening / closing means 82 of the blood component collection device 1 can enter, and the second tube 25b of the plasma collection bag 25 are exposed. The third in which the third channel opening / closing means 83 can enter.
Opening 93, third tube 2 of platelet collection bag 26
The connection between the fourth opening 94, the second line 22, and the fourth tube 27 a of the buffy coat collection bag 27, which exposes 6 a and allows the fourth flow path opening / closing means 84 of the blood component collection device 1 to enter. Exposing the second line 22 located on the centrifugal separator side (upstream side) of the blood sample collection device 1
The fifth opening 95 through which the fifth flow path opening / closing means 85 can enter, between the connection portion with the first line 21 and the connection portion with the fourth tube 27a of the buffy coat collection bag 27 (the The sixth opening that exposes the second line 22 (downstream from the connection between the second line 22 and the fourth tube 27a) and allows the sixth flow path opening / closing means 86 of the blood component collection device 1 to enter. The portion 96 is provided with a seventh opening 97 that exposes the fourth line 24 and allows the seventh flow path opening / closing means 87 of the blood component collection device 1 to enter.

On the inner surface of the cassette housing 28, the above-mentioned branch connector is fixed. Further, near the side surface of the cassette housing 28, a line and a tube protruding from the side surface of the housing are held,
Further, a reinforcing tube for preventing bending at the housing portion is provided. Cassette housing 28
Is a box-shaped body in which the portion shown by the broken line in FIG. 2 can be stored. Then, the cassette housing 28
Is formed of a synthetic resin having a certain degree of rigidity. The blood component collection device 1 includes the cassette housing mounting portion (not shown). For this reason, by mounting the cassette housing 28 on the cassette housing mounting portion of the blood component collecting apparatus 1, each line and each tube of the portion exposed from the opening of the cassette housing 28 automatically correspond to the corresponding channel opening / closing means. Attached to.
This makes it easy to mount the circuit and quickly prepares to collect blood components. In addition, blood component collection device 1
Are provided with two pumps close to the cassette housing mounting portion. For this reason, the cassette housing 2
The mounting of the pump tube exposed from the pump 8 to the pump is also easy.

The centrifugal separator 20 provided in the blood component collection circuit 2 is usually called a centrifuge bowl, and separates blood components by centrifugal force. As shown in FIG. 3, the centrifugal separator 20 has a vertically extending tube 141 having an inlet 143 formed at an upper end thereof, and rotates around the tube 141, and is liquid-tightly sealed to the upper portion 145. Hollow rotor 14
And 2. A flow path (blood storage space) is formed in the rotor 142 along the bottom and the inner surface of the peripheral wall,
An outlet 144 is formed so as to communicate with an upper part of the flow path. In this case, the volume of the rotor 142 is, for example, about 100 to 350 ml. Rotor 142
Is rotated under predetermined centrifugal conditions (rotation speed and rotation time) set in advance by a rotor rotation drive device 10 provided in the blood component collection device 1. By the centrifugation conditions, the blood separation pattern in the rotor 142 (for example,
(The number of blood components to be separated) can be set. In this embodiment, as shown in FIG. 3, blood flows from the inner layer to the plasma layer 131 and the buffy coat layer 132 in the flow path of the rotor 142.
And the centrifugation conditions are set so as to be separated into the red blood cell layer 133.

Next, the blood component collecting apparatus 1 of the present invention shown in FIG. 4 will be described. The blood component collection device 1 includes a centrifuge drive device 10 for rotating the rotor 142 of the centrifuge 20, a first liquid feed pump 11 for the first line 21, and a third line 23. And a plurality of flow passage opening / closing means 81, 82, 83, 84, 8 for opening and closing the flow passage of the blood component collection circuit 2.
5, 86, 87, and a control unit 13 for controlling the centrifuge drive device 10, the first liquid feed pump 11, the second liquid feed pump 12, and the plurality of flow path opening / closing means. Further, the blood component collection device 1 is attached above the turbidity sensor 14 and the centrifuge 20 which are attached to the second line 22 on the centrifugal separator side (upstream side) from the connection portion 22a with the second tube 25b. And a weight sensor 16 for detecting the weight of the plasma collection bag 25. Also, the flow path opening / closing means 81, 82, 83, 84, 8
5, 86 and 87 are all connected to the control unit 13, and their opening and closing are controlled by the control unit 13. further,
The turbidity sensor 14, the optical sensor 15 mounted above the centrifuge 20, and the weight sensor 16 for detecting the weight of the plasma collection bag 25 are also electrically connected to the control unit 13 and output from them. Is input to the control unit 13. The control unit 13 has a control mechanism composed of, for example, a microcomputer and a rotor rotation speed calculation function, and the detection signals from the weight sensor 16, the optical sensor 15, and the turbidity sensor 14 are sent to the control unit 13 at any time. Is entered. The control unit 13 includes the turbidity sensor 14, the optical sensor 1
5. Based on the signal from the weight sensor 16, control the rotation, stop, and rotation direction (forward / reverse) of each pump, and, if necessary, open / close each flow path opening / closing means and rotate the centrifuge separator 10 Control (rotor rotation).

The first channel opening / closing means 81 is provided for opening / closing the first line 21 on the blood collection needle side from the pump tube 21g. Second channel opening / closing means 82
Is provided for opening and closing the first tube 25a of the plasma collection bag 25. The third channel opening / closing means 83
It is provided to open and close the second tube 25b of the plasma collection bag 25. The fourth channel opening / closing means 84 is provided for opening / closing the third tube 26 a of the platelet collection bag 26. The fifth flow path opening / closing means 85 is connected to the second line 22 at a position closer to the centrifugal separator (upstream side) than the connection 22 d between the second line 22 and the fourth tube 27 a of the buffy coat collection bag 27. It is provided for opening and closing. The sixth flow path opening / closing means 86 is provided between the connection portion 21e with the first line 21 and the connection portion with the fourth tube 27a (downstream from the connection portion between the second line 22 and the fourth tube 27a). ) Is provided to open and close the second line 22. Seventh channel opening / closing means 87
Is provided for opening and closing the fourth line 24. The channel opening / closing means includes a line or tube insertion portion, and the insertion portion has a clamp operated by a drive source such as a solenoid, an electric motor, and a cylinder (hydraulic or pneumatic). Specifically, a pneumatic cylinder clamp operated by pneumatic pressure is suitable. The clamp of the passage opening / closing means operates based on a signal from the control unit 13.

As shown in FIG. 3, the rotor driving device 10 holds a rotor rotation driving device housing 151 for accommodating the centrifugal separator 20, a leg 152, a motor 153 as a driving source, and the centrifugal separator 20. Disc-shaped fixed base 1
55. The housing 151 includes the leg 1
It is placed and fixed on the upper part of the reference numeral 52. Further, a spacer 15 is provided on the lower surface of the housing 151 by using a bolt 156.
7, a motor 153 is fixed. Motor 15
A fixed base 155 is fitted to the tip of the third rotating shaft 154 so as to rotate coaxially and integrally with the rotating shaft 154, and the bottom of the rotor 142 is fitted to the upper part of the fixed base 155. A recess is formed. In addition, centrifuge 2
0 is fixed to the housing 151 by a fixing member (not shown). Rotor rotation drive 10
Then, when the motor 153 is driven, the fixed base 155 and the rotor 142 fixed thereto are rotated, for example, at a rotation speed of three.
Spin at 000-6000 rpm.

Also, the rotor rotation driving device housing 1
On the inner wall of 51, the interface of the separated blood components in the centrifuge (for example, plasma layer 131 and buffy coat layer 132)
B, buffy coat layer 132 and erythrocyte layer 133
An optical sensor (interface sensor) 15 that optically detects the position of the interface (interface with the interface) is installed and fixed by a mounting member 158. The interface sensor 15 includes a centrifuge 2
An optical sensor that can scan in the vertical direction along the outer peripheral surface of 0 is used. This sensor includes a light source that irradiates light toward the shoulder portion of the centrifuge 20 and a light receiving unit that receives light reflected from the centrifuge bowl and returned. That is, a light-emitting element such as an LED or a laser and a light-receiving element are arranged in a row, and light reflected by a blood component of light emitted from the light-emitting element is received by the light-receiving element, and the amount of received light is photoelectrically converted. Is configured. Separated blood components (for example, plasma layer 131 and buffy coat layer 13)
Since the intensity of the reflected light differs according to 2), the position corresponding to the light receiving element in which the amount of received light has changed is detected as the position of the interface B. More specifically, the amount of light received by the light receiving unit when the light passing through the centrifuge 20 is filled with a transparent liquid (plasma or water) and when the light is filled with a buffy coat layer It is detected from the difference that the buffy coat layer has reached the light passing portion. The position at which the buffy coat layer is detected is adjusted by changing the position at which light passes through the inside of the bowl. Usually, once the light beam passing position is determined, it is fixed there.

The turbidity sensor 14 is for detecting the turbidity of the fluid flowing in the second line 22, and outputs a voltage value corresponding to the turbidity. Specifically, a low voltage value is output when the turbidity is high, and a high voltage value is output when the turbidity is low. The first liquid supply pump 11 to which the pump tube 21g of the first line 21 is attached and the second liquid supply pump 12 to which the pump tube 23a of the third line 23 is attached are roller pumps and peristaltic pumps. Non-blood contact type pumps such as are preferred. As the first liquid sending pump 11 (blood pump), a pump capable of sending blood in any direction is used. Specifically, a roller pump capable of normal rotation and reverse rotation is used.

The controller 13 collects the blood to which the anticoagulant has been added, separates the collected blood, and collects the separated plasma in a plasma collection bag. At least one plasma collection / circulation step including a plasma circulation step of circulating the plasma in the collected plasma collection bag through the centrifuge; and, after the plasma collection / circulation step is completed, the plasma circulation by the first liquid feeding pump A platelet collection step of accelerating the speed to allow the platelets to flow out of the centrifuge and collect the platelets in the platelet collection bag, and a blood return step of returning the blood in the centrifuge after the completion of the platelet collection step. It is to be done. Specifically, the control unit 13 operates the first liquid supply pump 11 and the second liquid supply pump 12 to collect blood to which the anticoagulant has been added, and the centrifugal separator driving device 1
Activate 0 (with the above calculated value or set value,
Rotating the rotor) to collect plasma from blood 25
A first plasma collection step of collecting a first predetermined amount of plasma, and then temporarily suspending the blood collection and operating the centrifuge driving device 10 (the above-described calculated value or setting). At a constant speed), and a plasma collection / constant speed circulation step comprising a constant speed plasma circulation step (constant speed circulation) in which the plasma in the plasma collection bag is circulated at a constant speed to the centrifuge 20. Then, the first liquid supply pump 11 and the second liquid supply pump 12 are operated to collect blood to which the anticoagulant has been added, and the centrifugal separator driving device 1
Activate 0 (with the above calculated value or set value,
A second plasma collection step of collecting plasma until a predetermined position (for example, a buffy coat layer) is detected by the interface sensor, and after completion of the second plasma collection step, the blood collection is temporarily stopped. And, by operating the centrifugal separator driving device 10 (by rotating the rotor at the above-described calculated value or set value), the acceleration in which the plasma in the plasma collection bag 25 is circulated while being accelerated to the centrifugal separator 20 is accelerated. Plasma circulation step (accelerated circulation)
After the plasma collection / acceleration circulating step comprising the following steps, the plasma circulating speed by the first liquid supply pump 11 is accelerated to cause platelets to flow out of the centrifugal separator 20 to collect platelets. A platelet collection step of collecting the blood in the bag and a blood return step of returning the blood in the centrifuge 20 after the platelet collection step are completed. In the second plasma collection step, the weight of the plasma bag is not detected because the detection is performed by the interface sensor.

Thus, during one platelet collection operation,
The plasma recirculation step of suspending the blood collection and recirculating the collected plasma to the centrifuge is performed at least twice, and the latter half of the plasma recirculation step is accelerated circulation. In this case, the blood cell layer and the buffy coat layer (BC layer) can be suppressed from being excessively compressed, platelets buried in the red blood cell layer can be raised, and can be taken into the BC layer. Further, since the BC layer itself also rises, the separation and alignment of platelets and leukocytes in the BC layer are promoted. For this reason,
It is possible to obtain a platelet-containing liquid (rich platelet plasma) with little leukocyte contamination and high platelet collection efficiency.

Further, the blood component collecting apparatus 1 of this embodiment
The control unit 13 of the centrifugal separator driving device 10 so that the platelet collection operation including the plasma collection / constant-speed circulation step, the plasma collection / accelerated circulation step, the platelet collection It controls the first liquid feed pump 11, the second liquid feed pump 12, and a plurality of flow path opening / closing means. Further, the control unit 13 of the blood component collection apparatus 1 according to the present embodiment determines the plasma circulation speed by the first liquid feeding pump 11 after the platelet collection step and before the blood return step, to the final speed in the platelet collection step. The buffy coat is discharged from the centrifugal separator 20 and the buffy coat is collected in the buffy coat collection bag 27. The buffy coat collecting step is not limited to the above-described method. For example, the plasma circulation speed by the first liquid feeding pump 11 is maintained at the final speed in the platelet collecting step, and the centrifuge 20 This may be performed by lowering the rotation speed of the rotor. Further, the buffy coat collecting step may be performed by making the plasma circulation speed by the first liquid feeding pump higher than the final speed in the platelet collecting step and lowering the rotation speed of the rotor of the centrifuge.

After the buffy coat collection step is completed, the first liquid feeding pump is operated so as to perform a buffy coat return step of returning the collected buffy coat into the centrifuge 20 before the next blood collection step. 11 and a plurality of flow path opening / closing means. And the control unit 13
In the platelet collection step, from the signal detected by the turbidity sensor 14 and the signal change pattern calculated from this signal, the platelet collection process is either a stored normal pattern or a plurality of exceptional patterns. It has a platelet collection process pattern determination function for determining whether or not the platelet collection step is completed at a time suitable for the pattern determined by the platelet collection process pattern determination function. In particular, in the control unit 13 of the blood component collection device of this embodiment,
At the time of the platelet collection step, the signal detected by the turbidity sensor 14, the slope of the signal change calculated from the signal detected by the turbidity sensor 14 input over time, and the circulation speed at the time of the platelet collection step are used. And a platelet collection process pattern judging function for judging whether the platelet collection process is one of a stored normal pattern or a plurality of (specifically, A to E) exceptional patterns. Then, at a time point suitable for each pattern, the platelet collection step is terminated.

In the blood component collecting apparatus of the present invention, the turbidity sensor 14 and the voltage signal of the turbidity sensor 14
3 is provided with an analog / digital converter (not shown) for conversion and transmission to the arithmetic unit 3. The control unit 13 includes an arithmetic unit for signal processing, a storage of signal data and a program for executing pattern recognition and judgment. A storage unit such as a RAM or a ROM for storage is provided. 5 to 10 are diagrams showing a change pattern of a signal detected by the turbidity sensor at the time of collecting platelets. FIG. 5 is a diagram showing a normal change pattern of a signal detected by the turbidity sensor at the time of collecting platelets. FIG. 6 is a diagram illustrating an example (exception pattern A) of an exception pattern of a signal detected by the turbidity sensor during platelet collection. FIG.
FIG. 9 is a diagram showing another example (exception pattern B) of the exception pattern of the signal detected by the turbidity sensor at the time of platelet collection. FIG. 8 is a diagram illustrating another example (exception pattern C) of the exception pattern of the signal detected by the turbidity sensor when collecting platelets. FIG. 9 is a diagram showing another example (exception pattern D) of an exception pattern of a signal detected by the turbidity sensor at the time of collecting platelets. FIG. 10 is a diagram illustrating another example (exception pattern E) of an exception pattern of a signal detected by the turbidity sensor at the time of collecting platelets. In the state where the platelets are flowing out, the signal of the turbidity sensor 14 has changed to show an increase or decrease with time,
A minute time interval (1/1000 second to 2 seconds, preferably 1
Looking at the signal change at (/ 100 sec to 1 sec), the slope of “−” at the time of the start of platelet outflow is “0” near the peak of platelet outflow. Thereafter, since the outflow of platelets gradually decreases, the slope changes to “+”. When the outflow of platelets is completely completed or the outflow of white blood cells and red blood cells starts, this inclination changes from “0” to “−” again. Therefore, the control unit 13 determines the signal change pattern by using the slope.

Then, the control section 13 ends the platelet collection step at a time point suitable for the pattern determined by the signal change pattern determination function. Specifically, in the normal change pattern shown in FIG. 5, the voltage sharply drops with the start of platelet outflow, reaches the lower limit peak where the voltage does not reach the erythrocyte detection voltage, and then the voltage rapidly rises.
Exceeds end voltage. In a normal pattern, the change in slope is
Start of platelet collection → “−” → “0” → “+” → “0”. Then, such a pattern is taken, and the circulation speed at the time of starting platelet collection is 200 ml / min.
If it is less than the normal pattern, the platelet collection is terminated when the end voltage is reached.

One example of the exception pattern (exception pattern A) shown in FIG. 6 is a case where the turbidity sensor voltage does not reach the predetermined value X and the voltage gradually decreases as platelet outflow starts. This pattern is a state in which the platelets flow out but the amount of outflow is extremely small, and may not reach the target number of collected platelets. In this exceptional pattern A, the change in the slope is due to the start of platelet collection →
It becomes "-". In the case of such an exception pattern A,
Platelet concentration (PC concentration) is a predetermined amount (for example, 1 million)
Is stopped (the turbidity sensor voltage does not reach the predetermined value X), and the platelet collection is terminated when the platelet collection amount reaches the set amount. The reason for terminating here is that the outflow of platelets is extremely small, and the target platelet count cannot be collected even if the amount of collected platelets is increased.

The exception pattern B shown in FIG. 7 is a case where the voltage sharply drops with the start of platelet outflow, does not reach the lower limit peak even if it exceeds the red blood cell detection voltage, and the voltage continues to drop. This pattern indicates a situation in which the conditions for collecting platelets have been significantly deviated, and white blood cells and red blood cells have begun to flow out. In exceptional pattern B, the change in slope is
Platelet collection started → "-". In the case of such an exception pattern B, after the platelet concentration (PC concentration) has reached a predetermined amount (for example, 1,000,000) (the turbidity sensor voltage has reached a predetermined value X), the turbidity sensor voltage is At the time when the voltage reaches the red blood cell detection voltage, platelet collection is terminated.
This can prevent red blood cells from being mixed.

The exceptional pattern C shown in FIG. 8 is a case where the voltage sharply drops at the start of platelet outflow and does not exceed the red blood cell detection voltage, but continues to drop. In this pattern, although the outflow of platelets is not so small as in pattern A, it is a situation where the outflow of platelets is smaller than the value that should be outflow. In this case, the target platelet count is reached, but platelet collection conditions are still insufficient. In the exceptional pattern C, the change of the inclination is “platelet collection start →“ − ”. In the case of such an exception pattern C, after the platelet concentration (PC concentration) has reached a predetermined amount (for example, 1,000,000) (the turbidity sensor voltage has reached a predetermined value X), the turbidity sensor voltage is increased. The platelet collection is terminated when the platelet collection amount has reached the set amount without reaching the red blood cell detection voltage and without obtaining the lower limit peak of the voltage. The reason for terminating the process is that the outflow of platelets is decreasing, and if platelets are continuously collected as it is, the concentration of platelets in the bag will decrease.

In the exceptional pattern D shown in FIG. 9, the voltage suddenly decreases with the start of platelet outflow, and after reaching the lower limit peak, the voltage increases but does not reach the end voltage. In this pattern, although the outflow of platelets is not so small as in pattern A, it is a situation where the outflow of platelets is smaller than the value that should be outflow. In this case, the target platelet collection number is reached, but the platelet collection conditions are still insufficient. In the exceptional pattern D, the change in the slope indicates the start of platelet collection →
“−” → “0” → “+”. In the case of such an exception pattern D, after the platelet concentration (PC concentration) has reached a predetermined amount (for example, 1,000,000) (the turbidity sensor voltage has reached a predetermined value X), the turbidity sensor voltage is increased. After the erythrocyte detection voltage has not been reached and the lower limit peak of the voltage has been obtained, the platelet collection is terminated when the platelet collection amount reaches the set amount without the turbidity sensor voltage reaching the end voltage. The reason for terminating is that the lower voltage peak has been obtained and a certain number of platelets has been secured. However, even if platelet collection is continued as it is, the collection efficiency will deteriorate (it takes time until the end voltage is reached). Because the platelet concentration in the bag is lower).

In the exceptional pattern E shown in FIG. 10, the voltage sharply drops with the start of platelet outflow, reaches a lower limit peak when the voltage does not reach the erythrocyte detection voltage, and then the voltage rises sharply without exceeding the end voltage. In this case, the voltage drops again. In this pattern, the change in the slope is from the start of platelet collection → “−” → “0” → “+”
→ "0" → "-". In this case, the platelet collection is terminated when the change in the voltage gradient reaches “−” without reaching the end voltage. This pattern E
In this case, the supplied platelets are not so large, and the outflow of white blood cells and red blood cells occurs following the outflow of platelets. As described above, by combining the method of analyzing the signal of the turbidity sensor with the passage of time and the detection signal of the turbidity sensor, the collection of platelets can be completed before white blood cells or red blood cells flow out.

Further, the control unit 13 has a platelet collection condition adjusting function for adjusting the platelet collection condition in the next platelet collection step when the exception is determined by the platelet collection process pattern determination function. Is preferred. The platelet collection condition adjusting function of the control unit 13 is performed by a platelet collection process pattern determination function.
When it is determined that the condition is one of the plurality of exceptional patterns, it is preferable to adjust the platelet collection conditions to conditions suitable for the determined exceptional pattern in the next platelet collection step. In particular, the platelet collection condition adjustment function preferably adjusts the platelet collection conditions so that the change pattern of the signal detected by the turbidity sensor during the platelet collection step approaches the stored normal pattern. .

Specifically, at the time when the platelet collection is completed, the parameters to be fine-tuned from the platelet collection pattern in the next cycle [actually, the second platelet collection Operation (Next Platelet Collection Step)] Automatically select the fine adjustment amount (the detection timing of the BC interface sensor for the end) (the fine adjustment amount is set in advance as a table value corresponding to the outflow pattern). That is, the detection timing of the BC interface by the sensor 15 for ending the next platelet collection operation (platelet collection step) is changed according to the determined pattern. More specifically, in the case of the exceptional pattern A as shown in FIG. 6, the detection setting of the BC interface is lowered. Lowering the BC interface detection setting means increasing the blood collection volume,
An operation for raising the BC interface position in the centrifugal bowl toward the outflow port is shown. Specifically, it is to increase the detection value (light receiving rate%) of the BC interface sensor. The decrease in the detection setting (detection rate) of the BC interface is preferably about 0.5 to 5%, and more preferably about 1 to 2%. Also, BC
By lowering the interface, the signal change of the turbidity sensor 14 approaches a normal pattern, so that the efficiency of platelet collection is improved.

In the case of the exceptional pattern B as shown in FIG. 7, the detection setting of the BC interface is increased. Raising the BC interface indicates an operation for reducing the amount of blood supplied into the centrifuge bowl (reducing the amount of blood collected) and lowering the BC interface position in the centrifuge bowl toward the inflow port. To increase the detection value (light reception rate%). BC
The decrease in the interface detection setting (detection rate) is 0.5 to
It is preferably about 5%, more preferably about 1 to 2%. By raising the BC interface in this way, the signal change of the turbidity sensor 14 approaches a normal pattern, so that the efficiency of platelet collection is improved.

In the case of the exception pattern C shown in FIG. 8 and the exception pattern D shown in FIG. 9, the detection setting of the BC interface is lowered. As a decrease in the detection setting of the BC interface,
It is preferably about 0.5-5%, more preferably 1-2.
%. Further, it is preferable that the reduction width is adjusted by the signal voltage (final voltage) of the turbidity sensor at the time of collecting platelets. That is, when the final voltage is lower than the predetermined value, it is preferable to make the drop width larger than when the final voltage is higher than the predetermined value. By lowering the BC interface in this way, the signal change of the turbidity sensor 14 approaches a normal pattern, so that the efficiency of collecting platelets is improved.

In the case of the exceptional pattern E shown in FIG. 10, the detection setting of the BC interface is increased. It is preferable that the raising width is adjusted by the circulation speed at the time of collecting platelets. In other words, the circulation speed at the end is a predetermined value (for example, 130 ml
/ Min) or less, it is preferable to make the raising width larger than when it is higher than a predetermined value. Further, the circulation speed at the time of termination is changed to a second predetermined value (for example, 200 ml / mi).
n) In the case described above, the detection setting of the BC interface may not be changed. By raising the BC interface in this way, the signal change of the turbidity sensor 14 approaches a normal pattern, so that the efficiency of platelet collection is improved. As described above, by adjusting, the change pattern of the signal detected by the turbidity sensor during the platelet collection step,
It comes closer to the memorized normal pattern.

Some blood donors show a tendency for platelet outflow to decrease as platelet collection proceeds. In the case of such a donor, there is a case where blood cell outflow occurs without showing a peak of platelet outflow in the second and subsequent platelet collection steps. Therefore, the data in the platelet collection step at the time of the first platelet collection operation, which was normal, is stored and compared with the data at the platelet collection step at the time of the second and subsequent platelet collection operations. When there is a difference, platelet collection is interrupted to prevent blood cell contamination. For this purpose, the control unit uses the signal detected by the turbidity sensor, the signal change pattern calculated from the signal, and the circulation speed at the time of platelet collection in the platelet collection step to perform the platelet collection process. It is to determine whether the stored normal pattern or any of a plurality of exceptional patterns, further, the platelet collection process pattern determination function, when it is determined that the first platelet collection process is a normal pattern, An initial platelet collection process data storage unit that stores data in the initial platelet collection step, and a data comparison function for comparing data in the platelet collection process in the next and subsequent platelet collection steps with the first platelet collection process data, The adjustment function at the end of the platelet collection step of the control unit is It is preferable that the difference equal to or greater than a predetermined between platelet collection process data for the first time platelet collection process the data comparison target times has the function to terminate the platelet collection step if it is detected Ri.

Further, the blood component collecting apparatus is provided with a warning function which is activated when a difference equal to or more than a predetermined value is detected between the initial platelet collecting process data and the comparison target platelet collecting process data by the data comparing function. Is preferred. Specifically, the control unit measures and stores the peak time from the start of the platelet collection step to the detection of the lower limit peak voltage by the turbidity sensor during the first platelet collection operation, and a storage function of the lower limit peak voltage, Second
A comparison function for comparing whether the difference between the turbidity sensor voltage and the lower limit peak voltage at the time of the peak time from the start of the platelet collection step in the subsequent platelet collection operation is greater than a predetermined value. Further, the control unit, as part of the adjustment function at the end of the platelet collection step,
The comparison function has determined that the difference between the turbidity sensor voltage and the first lower limit peak voltage at the time of reaching the peak time from the start of the platelet collection step in the second and subsequent platelet collection operations is greater than a predetermined value. In this case, the voltage change rate of the turbidity sensor for a predetermined second is calculated, and if the calculated voltage change rate is smaller than a predetermined value, the platelet collection step is terminated.

The control function of the control unit at the end of the platelet collection step is performed by the voltage change rate calculating function again when the voltage change rate in the predetermined second calculated by the voltage change rate calculating function is larger than the predetermined value. It is preferable that a second voltage change rate in a predetermined second is calculated, and the platelet collection step is terminated when the calculated second voltage change rate is smaller than a predetermined value. Further, it is preferable that the blood component collection device has a warning function that is activated when a difference of not less than a predetermined value is detected between the initial platelet collection process data and the comparison target platelet collection process data by the data comparison function. Specifically, it is preferable to have a warning function that operates when the calculated second voltage change rate is smaller than a predetermined value. Specifically, the platelet collection device has the following adjustment function at the end of the platelet collection step.

First, the time from the start of platelet collection to the peak of platelet outflow (peak time: Tpeek1) and the peak voltage (Vpeek1) by the turbidity sensor are measured and stored in the platelet collection step in the first platelet collection operation. .
Then, in the platelet collection step in the second and subsequent platelet collection operations, the turbidity sensor voltage (V2) at the time when the first peak time (Tpeek1) has been reached from the start of platelet collection is stored. Then, when the lower limit peak is not detected by the turbidity sensor, it is compared whether V2 is larger than Vpeek1 + predetermined value (in other words, allowable value, for example, 0.1 to 1.0 v). In other words, V2-Vpeek1 is
It is compared whether it is greater than a predetermined value (allowable value). If the difference is larger than the predetermined value, it is determined that there is an abnormal tendency.

In the case of "abnormal tendency", it is determined whether or not to end the platelet collection step using the rate of change or the amount of change in the turbidity sensor voltage for a predetermined second. Specifically, the turbidity sensor voltage (V2a) after a lapse of a predetermined time (for example, about 1 to 5 seconds) and the turbidity sensor voltage (V2) when the first peak time (Tpeek1) is reached
The turbidity sensor voltage change rate during a predetermined time (predetermined second) is calculated, and it is determined whether the turbidity sensor voltage change rate is greater than a first allowable value (for example, -0.5 to +0.5 v). . When the turbidity sensor voltage change rate is equal to or less than the first allowable value, the platelet collection step is terminated. When the turbidity sensor voltage change rate is larger than the first allowable value, the turbidity sensor voltage (V2b) after a lapse of a predetermined time (for example, about 1 to 5 seconds) and the turbidity sensor voltage ( V2a), the turbidity sensor voltage change rate for a predetermined time (predetermined second) is calculated, and the turbidity sensor voltage change rate is set to a second allowable value (for example, -0.5 to +0.5).
v) Determine if it is greater than. When the turbidity sensor voltage change rate is equal to or less than the second allowable value, the platelet collection step is terminated. If the turbidity sensor voltage change rate is larger than the second allowable value, the process returns to the normal program and continues the platelet collection step. Thus, 2
In the platelet collection step in the subsequent and subsequent plasma collection operations, the data on
Judge the situation in the subsequent platelet collection step,
In the case of an abnormal state, platelet collection with less blood cell contamination can be performed by terminating the platelet collection step. Note that the above function is also effective for a platelet collection device that does not have a platelet collection process pattern determination function and a platelet collection step end adjustment function based on the determination function.

Such a platelet collecting apparatus is a blood component collecting apparatus for separating blood into a plurality of blood components by a centrifugal separator having a blood storage space therein and collecting a specific blood component into a bag. The blood component collection device includes a first line for connecting a blood collection unit and an inlet of the centrifuge, a second line connected to an outlet of the centrifuge, and the first line. A plasma collection bag having a first tube connected in the middle of the first tube and a second tube connected to the second line, and a blood component collection circuit including a platelet collection bag connected to the second line,
A turbidity sensor attached to an outlet or the second line of the centrifuge; a centrifuge drive device for rotating a rotor of the centrifuge; and a control unit, wherein the control unit includes Sampling of blood to which a coagulant has been added, separation of the collected blood and collection of the separated plasma in the plasma collection bag, and plasma in the plasma collection bag collected by the plasma collection step At least one plasma collecting and circulating step comprising a plasma circulating step of circulating the plasma to the centrifugal separator, and, after the plasma collecting and circulating step is completed, accelerating the plasma circulating speed by the first liquid sending pump. Performing a platelet collection step of causing the platelets to flow out of the centrifuge and collecting the platelets in the platelet collection bag. The control section controls the platelet collection operation to perform a blood return step of returning blood in the centrifuge to be performed at least twice, and further, the control section controls the platelet collection operation during the first platelet collection operation. The function of measuring and storing the peak time from the start of the platelet collection step to the detection of the lower limit peak by the turbidity sensor, and the storage function of the lower limit peak voltage, and reaching the peak time from the start of the platelet collection step during the second and subsequent platelet collection operations A comparison function for comparing whether or not the difference between the turbidity sensor voltage and the lower limit peak voltage at the time is greater than a predetermined value, and the comparison function allows the peak to be measured from the start of the platelet collection step during the second and subsequent platelet collection operations. The difference between the turbidity sensor voltage and the lower limit peak voltage at the time of arrival is greater than a predetermined value When it is determined, the voltage change rate calculation function for calculating the turbidity sensor voltage change rate for a predetermined second, and when the voltage change rate calculated by the voltage change rate calculation function is smaller than a predetermined value, the platelet collection step is ended. An adjustment function at the end of the platelet collection step to be performed is provided. The platelet collection device and the blood component collection device are the same as those described above, except that the platelet collection process pattern determination function and the platelet collection step end adjustment function based on the determination function are not provided.

The platelet collection operation will be described more specifically.
Anticoagulant prescribed for whole blood (1/8 to 1/2 of whole blood)
0, specifically 1/10), and a predetermined speed (25
0 ml / min or less; preferably 150 to 40 ml
/ Min or less, specifically 60 ml / min or less) to the centrifugal separator 20 via the first line 21 to rotate the centrifugal separator 20 at a predetermined rotation speed (3000 to 6000 rpm,
Preferably, the blood is rotated in the range of 4400 to 5800 rpm to separate the blood into plasma, buffy coat, and red blood cell components. ,
(Preferably, 20 to 30 ml), the blood supply is stopped at the time of collection, and the plasma is pumped under predetermined conditions (at a rate larger than the blood collection volume, 10 to 90 sec at 60 to 250 ml / min, specifically, the first circulation). Is 200ml / min × 30se
At c), a constant-rate plasma circulation is performed, returning to the centrifuge 20 through the first line 21 and the second line 22.

Then, an anticoagulant is again added to whole blood (1/8 to 1/20, specifically 1/10 with respect to whole blood).
At a predetermined rate (250 ml / min or less; preferably, 150 to 40 ml / min or less, specifically,
(60 ml / min or less) to the centrifugal separator 20 via the first line 21 to rotate the centrifugal separator 20 at a predetermined rotation speed (3
000-6000 rpm, preferably 4700-48
00 rpm) to separate blood into plasma, buffy coat, and red blood cell components. When the blood cell interface position inside the centrifuge 20 is detected by the buffy coat interface detection sensor, the blood supply is stopped. Under predetermined conditions (initial speed
80 ml / min, final arrival speed (set speed) 150 to
250 ml / min, acceleration conditions (every second) 2-1
0 ml / min speed increase, circulation time 10 to 90 sec
In c), an accelerated plasma circulation is performed, returning to the centrifuge 20 through the first line 21 and the second line 22. After the last blood collection, the plasma is first and second
Return to the centrifugal separator 20 through the line 22, and increase the acceleration stepwise under predetermined conditions (stepwise acceleration; 0.1 to 99 ml / min / sec, specifically,
2 to 10 ml / min / sec) Platelet collection speed (60
２５０250 ml / min), and collects outflowing platelets from the centrifuge 20 into the platelet collection bag 26. In particular, in the blood component collection device of the present invention, the platelet collection process can be performed based on the signal detected by the turbidity sensor 14 and the signal change pattern calculated from the signal, whether the stored normal pattern or a plurality of exceptional patterns. Is determined, and the platelet collection step ends at a time point suitable for the determined pattern.

Further, in this apparatus, after collecting the platelets, the blood circulation speed is maintained (60 to 250 ml / min, specifically, 200 ml / min), and the centrifuge 20
Lower the number of revolutions (100-30
By lowering the buffy coat by about 0 rpm), the buffy coat that has flowed out is collected, and the collected buffy coat is supplied to the centrifugal separator 20 before performing blood collection in the next cycle. The buffy coat may be collected by accelerating the blood circulation speed to a predetermined speed (equal to or higher than the platelet collection speed, preferably 60 to 250 ml / min, specifically 205 ml / min) after collecting the platelets. Good.

FIG. 11 shows a blood component collecting step (first platelet collecting operation) by the blood component collecting apparatus 4 of this embodiment.
This will be described with reference to the flowchart of FIG. In this embodiment, the platelet collection operation is repeated twice, and further, after the end of the platelet collection step other than the last one, before the blood return step, the buffy coat collection step is performed, and before the next blood collection step. A buffy coat return step for returning the buffy coat to the centrifuge 20 is performed. The seventh flow path opening / closing means 87 is closed, and the fourth line 24 for injecting fluid is not used.
First, the third line 23 and the blood collection needle 29 are primed with an anticoagulant, and then the donor is punctured with a puncture needle. Then, the centrifuge drive device 10 performs a first plasma collection step of rotating the rotor to collect a first predetermined amount of plasma from the blood into the plasma collection bag 25.

When the first blood collection is started, the blood pump 1
1 starts blood collection at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, is also simultaneously driven at a predetermined speed (eg, 1/1 of the blood pump speed).
At 0), an anticoagulant (for example, ACD-A solution) is supplied.
Blood collected from the donor is mixed with ACD-A solution,
It flows through the first line 21, passes through the chamber, the first channel opening / closing means 81, and flows into the centrifuge 20. At this time, the sixth flow path opening / closing means 86 and the fifth flow path opening / closing means 8
5. Second channel opening / closing means 82, third channel opening / closing means 83
Is closed, and the first channel opening / closing means 81 and the fourth channel opening / closing means 84 are open. ACD-A in the centrifuge 20
When the liquid-added blood (ACD-added blood) is supplied, the sterilized air that has entered the centrifuge 20 flows through the second line 22, passes through the fourth channel opening / closing means 84, and enters the platelet collection bag 26. Flows into. Simultaneously with the start of the blood collection step, the centrifuge 20 starts rotating at a predetermined speed, and the centrifuge 20 receives the supply of ACD-added blood while rotating, so that blood is centrifuged in the separator, and the blood is Plasma layer from the inside,
ACD blood (about 270 m) that is separated into three layers, a buffy coat layer (BC layer) and an erythrocyte layer, and exceeds the capacity of the separator
When 1) is supplied, the inside of the centrifuge 20 is completely filled with blood, and the plasma flows out from the outlet of the centrifuge 20. The turbidity sensor 14 attached to the second line 22 connected to the outlet of the centrifuge 20 detects that the fluid flowing in the line has changed from air to plasma.
The control section 13 closes the fourth flow path opening / closing means 84 and opens the third flow path opening / closing means 83 based on the detection signal of the turbidity sensor 14, and collects the plasma in the plasma collection bag 25. I do. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. For this reason, the weight of the plasma collected in the plasma collection bag 25 is the first predetermined amount (10 to 1).
When the control unit 13 increases by 50 g, for example, 30 g),
The first flow path opening / closing means 81 is closed and the second flow path opening / closing means 82 is opened, and the process proceeds to a constant-speed plasma circulation step.

In the constant-velocity plasma circulation step, the blood collection is temporarily stopped, and the centrifuge driving device 10 is operated to remove the plasma collected in the plasma collection bag 25 to the centrifugal separator 20.
At a constant speed. When entering the constant-speed plasma circulation step, the control unit 13 maintains the closed state of the first flow path opening and closing means 81 and the open state of the second flow path opening and closing means 82,
The CD pump 12 stops, and the blood pump 11 operates at a predetermined speed (60 to 250 ml / min, for example, 200 ml / m).
In), the plasma in the plasma collection bag 25 is sent to the centrifugal separator 20 rotating at a predetermined speed through the second channel opening / closing means 82. At the same time, the plasma flowing out of the centrifuge 20 flows into the plasma collection bag 25 through the turbidity sensor 14 and the third channel opening / closing means 83. A predetermined time (10 to 90 seconds, e.g., 3
0 seconds), the control unit 13 closes the second flow path opening / closing means 82, opens the first flow path opening / closing means 81,
To the plasma collection step. The first plasma circulation is preferably performed at a flow rate of at least 60 ml / min or more for 10 seconds or more.

In the second plasma collection step, the first liquid supply pump 11 and the second liquid supply pump 12 are operated to collect the blood to which the anticoagulant has been added, and usually, the amount of plasma in the bag is reduced. When the optical sensor 15 detects the buffy coat layer of the separator due to the increase, this signal is sent to the control unit 13, and the control unit 13 closes the first flow path opening / closing means 81, and The road opening / closing means 82 is opened, and the process proceeds to the accelerated plasma circulation step. Specifically, the first liquid supply pump 11 starts collecting blood at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, is also simultaneously driven at a predetermined speed (eg, 1/1 of the blood pump speed).
At 0), an anticoagulant (for example, ACD-A solution) is supplied.
Blood collected from the donor is mixed with ACD-A solution,
The blood flows into the centrifugal separator 20 rotating at a predetermined speed, and the plasma is collected in the plasma collection bag 25. Usually, when the optical sensor 15 detects the buffy coat layer of the separator due to an increase in the amount of plasma in the bag, this signal is sent to the control unit 13, and the control unit 13 Is closed, the second flow path opening / closing means 82 is opened, and the process proceeds to the accelerated plasma circulation step. In the plasma collection step, plasma is collected until the sensor 15 detects a buffy coat (BC interface: interface between the plasma layer and the buffy coat layer). In the apparatus of this embodiment, FIGS.
As shown in the flowchart of FIG. 8, the detection of the BC interface is performed in each plasma collection step, and if the BC interface is detected during the first plasma collection step, the plasma collection is interrupted. Then, move to the accelerated plasma circulation step.

In the accelerated plasma circulation step, the blood collection is temporarily stopped, and the centrifuge driving device 10 is operated to circulate the plasma in the plasma collection bag 25 to the centrifuge 20 while accelerating. At this time, the blood pump speed is lower than the constant speed plasma circulation step, for example, 60 ml / mi.
n and the final speed is 150-200 ml / mi
Accelerate until n is reached. The acceleration conditions are as follows: a speed of 2 to 10 ml / min increases every second, 200 m
It is performed with an arrival time of 1 / min about 14 to 70 seconds. After the end of the circulation step, the process proceeds to FIG. 12, and a small amount plasma collection step for interface adjustment is performed.

As shown in FIG. 12, in the step of collecting a small amount of plasma for interface adjustment, in order to keep the position of the buffy coat layer constant in the subsequent platelet collection step regardless of the donor, only a predetermined supply amount of red blood cells is provided. Collect blood. The red blood cell supply amount is defined as a value obtained by dividing the blood collection amount by the hematocrit value of the donor, and the blood collection amount is generally about 12 ml. Also in this blood collection, the first liquid supply pump 11 starts blood collection at a predetermined speed (for example, 60 ml / min). At this time,
The second pump, which is an anticoagulant pump, simultaneously supplies an anticoagulant (for example, ACD-A liquid) at a predetermined speed (for example, 1/10 of the blood pump speed). Blood collected from the donor is mixed with the ACD-A solution, flows into the centrifugal separator 20 rotating at a predetermined speed, and a small amount of plasma is collected. The control unit 13 calculates the collection time from the set collection amount and the pump speed, and ends the blood collection when the collection time has elapsed. Then, the control unit 13 closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to a platelet collection step.

After the above steps are completed, the plasma circulation speed of the first liquid feed pump 11 is increased, and the centrifuge 20
Platelets are drained from the inside and platelets are collected in a platelet collection bag 26.
A platelet collection step of collecting platelets. The platelet collection step is also called a so-called acceleration process. In this step, initially, as shown in FIG.
The control unit 13 operates the blood pump so as to accelerate from 0 ml / min to 200 ml / min by 2 ml / min every predetermined time (for example, 1 second), and to operate at 200 ml / min.
min, until the platelet collection process ends
Maintain that speed.

When the platelet collection step starts, the turbidity sensor 14 detects the turbidity of the liquid passing therethrough, and the turbidity is output as a voltage value by the sensor.
3 is input. Blood pump speed increased to 120m
After reaching 1 / min, it is accelerated by 2 ml / min every predetermined time (for example, 1 second) until it is determined from the voltage value of the turbidity sensor that the PC concentration is 300,000 or more. When it is determined that the PC concentration is 300,000 or more,
Shift to FIG. The circulation speed is 200 ml /
If the PC concentration does not become 300,000 or more even after a predetermined time (for example, 10 seconds) has elapsed after reaching min, the collection of platelets is terminated as an abnormal pattern, and the process proceeds to FIG. In addition, the circulation speed at the time of outflow of platelets is referred to as outflow speed.

When platelets flow out, the turbidity of the liquid passing through the turbidity sensor 14 increases, and it is determined from the voltage value output from the sensor whether the PC concentration is 1,000,000 or more. If the PC collection amount (platelet collection weight) has reached the set value,
The platelet collection is terminated, and it is recognized that the exception pattern A has been met. Also, the PC concentration reaches 1,000,000 or more from the voltage value output from the sensor, and thereafter,
When the PC concentration (sensor voltage value) reaches the RBC level (red blood cell detection voltage), platelet collection is terminated at that time, and it is recognized that the exception pattern B has been met. Also, from the voltage value output from the sensor,
If the PC concentration reaches 1,000,000 or more, and then the sensor voltage value does not reach the lower limit peak (PC concentration is the highest value) and the PC collection amount (platelet collection weight) reaches the set value, then the time , The platelet collection is terminated, and it is recognized that the exception pattern C has been met. Also, the PC density is 10% from the voltage value output from the sensor.
If the sensor voltage reaches the lower limit peak (PC concentration is the highest value) after that, the sensor voltage reaches P0,000 or more, and thereafter, P
The slope of the C outflow curve (the slope of the sensor voltage value change) is continuously calculated. Then, after that, when the PC collection amount (platelet collection weight) reaches the set value, at that time,
The platelet collection is terminated, and it is recognized that the exception pattern D has been met. If the PC collection amount (platelet collection weight) reaches the set value in a state where the slope of the PC outflow curve (slope of sensor voltage value change) is continuously calculated, the pattern falls under the normal pattern. At the same time, the platelet collection is terminated at that time. Further, in a state where the slope of the PC outflow curve (the slope of the sensor voltage value change) is continuously calculated, the PC voltage (sensor voltage value) does not reach the set value of the PC collection amount (platelet collection weight). If the slope of the PC outflow curve (the slope of the sensor voltage value change) changes from “+” to “0” without reaching the end voltage, platelet collection is terminated at that time, It is recognized that the exception pattern E has been met. In any of the above cases, the third channel opening / closing means 83 is closed and the fourth channel opening / closing means 84 is closed.
Opens, and the platelet-rich plasma flowing out of the centrifuge 20 is collected in the platelet collection bag 26. Further, the voltage value output from the turbidity sensor 14 is converted into a platelet concentration by the control unit 13, and the platelet concentration in the platelet collection bag 26 during platelet collection is calculated.

When the platelet collection is completed, the process proceeds to the buffy coat collection step, and the process also proceeds to FIG. 14, where post-processing according to the corresponding pattern is performed in the platelet collection process. When proceeding to the post-processing decision, it is analyzed which of the platelet collection process patterns corresponded,
In the case other than the normal pattern, the next BC interface change condition is determined. Specifically, in the case of exception pattern A,
Reduce the BC interface detection setting by 2%. In the case of the exceptional pattern B, the detection setting of the BC interface is increased by 2%. In the exceptional patterns C and D, when the signal voltage (final voltage) of the turbidity sensor at the time of platelet collection is 2 V or more, the voltage is lowered by 2%, and when it is 1-2 V, it is lowered by 3%.
In the case of the exceptional pattern E, the circulation speed (outflow speed) is
If it is less than a predetermined value (for example, 130 ml / min),
2% increase, if it is within a predetermined range (for example, from 130 ml / min to less than 200 ml / min), increase by 1%, and if the outflow speed is 200 ml / min or more, do not change the BC interface detection setting. And Then, the BC interface parameters are automatically rewritten according to the above change conditions.

In the buffy coat collecting step, when the above-described platelet collecting step is completed, the controller 13
Is closed, and the fifth channel opening / closing means 85 is opened. The blood plasma in the plasma collection bag 25 is sent to the centrifugal separator 20 by the blood pump 11, and the liquid (the buffy coat layer has flowed out) from the centrifuge 20 simultaneously flows into the buffy coat collection bag 27. .
In the buffy coat collecting step, the speed of the blood pump 11 is maintained at the final speed in the platelet collecting step, and the centrifugal separator 20 is rotated at a predetermined speed or a rotation speed slightly higher than the predetermined speed (50 to more than the predetermined speed). 2
The rotation speed is increased to about 00 rpm (for example, a rotation speed higher by 100 rpm), whereby the buffy coat flows out from the centrifuge 20 and is collected in the buffy coat collection bag 27. When the amount calculated from the donor's hematocrit value and the amount of collected platelets is collected, the blood pump 11 stops, all valves close, the rotation of the centrifuge 20 stops, and the buffy coat collection step ends.

Next, a blood return step of returning the blood in the centrifuge 20 is performed. The control unit 13 rotates the blood pump 11 in the reverse direction, opens the first channel opening / closing unit 81,
The red blood cell layer remaining in the centrifuge 20 is transferred to the first line 2
Blood is returned to the donor from 1. This is the first time (first time)
When the platelet collection operation is completed, the process proceeds to the step shown in FIG. 15, and a second platelet collection operation is performed. First, as shown in FIG. 15, a buffy coat return step of returning the buffy coat collected by the first platelet collection operation into the centrifuge 20 before the next plasma collection step is performed. When the process proceeds to the buffy coat return step, the control unit 13 rotates the centrifugal separator 20 at a predetermined rotation speed (calculated value of the initial rotor rotation speed), and the fifth flow passage opening / closing device 85 and the fourth flow passage opening / closing device 84 Is opened, and the blood pump 11 is set to a predetermined speed (the default is 100 ml / mi).
Operate in n). The buffy coat contained in the buffy coat collection bag 27 is supplied to the centrifugal separator 20 through the fifth channel opening / closing means 85. The air in the centrifuge 20 is sent to the platelet collection bag 26 through the second line 22 and the fourth channel opening / closing means 84. After the blood pump 11 rotates by the amount of the buffy coat collected, the buffy coat return step ends.

Then, the flow shifts to that shown in FIG. 16, and a small amount plasma collection step, a platelet collection step, a buffy coat collection step, and a blood return step are sequentially performed, and the second platelet collection operation is completed. In this platelet collection step, as shown in FIG. 16, in the first platelet collection step, if the BC interface parameters are automatically rewritten, the correction condition is not used. In the method, platelets are collected under the initial conditions. In addition, after the acceleration starts, if the density change is proper, the process proceeds to FIG. 17 (if the pattern is abnormal, the process proceeds to FIG. 17)
Then, when the actual collection of platelets is started,
In the second platelet collection step, the platelet collection process pattern is determined, the platelet collection is terminated at a time suitable for each pattern, and the BC interface parameters are automatically rewritten when the pattern is recognized as an exception pattern. Will be

Next, the final platelet collection operation shown in FIG. 18 will be described. In this embodiment, the third time is the final time, but the present invention is not limited to this, and the fourth and subsequent times may be the final time of the platelet collection operation. In this case, except for the last time, the operation is the same as the second platelet collection operation (FIGS. 16 and 17). First, as shown in FIG. 18, the buffy coat collected by the second platelet collection operation was centrifuged into a centrifuge 20 before the next plasma collection step.
Perform a buffy coat return step to return within. When shifting to the buffy coat return step, the control unit 13
The centrifuge 20 is rotated at a predetermined rotation speed (for example, 4800 rpm).
m) to open the fifth flow path opening / closing means 85 and the fourth flow path opening / closing means 84, and operate the blood pump 11 at a predetermined speed (the default is 100 ml / min). The buffy coat contained in the buffy coat collection bag 27 is supplied to the centrifugal separator 20 through the fifth channel opening / closing means 85. The air in the centrifuge 20 is supplied to the second line 2
2. It is sent to the platelet collection bag 26 through the fourth channel opening / closing means 84. After the blood pump 11 rotates by the amount of the buffy coat collected, the buffy coat return step ends.

Next, the first liquid feed pump 11 and the second liquid feed pump 12 are operated to collect the blood to which the anticoagulant has been added, and the centrifuge driving device 10 is operated to remove the blood. A first plasma collection step of collecting a first predetermined amount of plasma in the plasma collection bag 25 is performed. When the first blood collection is started, the first liquid supply pump 11 operates at a predetermined speed (for example, 60
(ml / min). At this time, the second pump, which is an anticoagulant pump, also has a predetermined speed (for example,
Anticoagulant (eg, AC at 1/10 of the blood pump speed)
(DA solution). The blood collected from the donor is A
The mixture is mixed with the CD solution, flows through the first line 21, passes through the chamber, the first channel opening / closing means 81, and
Flows into. At this time, the sixth channel opening / closing means 86 and the fifth
, The second channel opening / closing unit 82, the third channel opening / closing unit 83, and the seventh channel opening / closing unit 87 are closed, and the first channel opening / closing unit 81 and the fifth channel opening / closing unit 87 are closed. Channel opening / closing means 85
Is open, and when the ACD blood is supplied to the centrifuge 20, the sterilized air originally contained in the centrifuge 20 passes through the line sensor, the fifth flow path opening / closing means 85, and the buffy coat collection bag 27. Flows into. Simultaneously with the start of the blood collection process, the centrifugal separator 20 starts rotating at a predetermined speed (if the corrected rotor rotational speed has been calculated, if it has not been calculated, otherwise the initial rotor rotational speed calculated value). 20 receives the supply of ACD blood while rotating, the blood is centrifuged in the separator, and the blood is separated from the inside into three layers, a plasma layer, a buffy coat layer (BC layer), and a red blood cell layer. ACD blood over the capacity of the separator (about 2
70 ml), the inside of the centrifuge 20 is completely filled with blood, and the plasma flows out from the outlet of the centrifuge 20. The second connected to the outlet of the centrifuge 20
The turbidity sensor 14 attached to the line 22 detects that the fluid flowing in the line has changed from air to plasma, and the control unit 13 performs the fifth operation based on the detection signal of the turbidity sensor 14. The flow path opening / closing means 85 is closed, and the third
Is opened, and the plasma is collected in the plasma collection bag 25. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. Therefore, when the weight of the plasma collected in the plasma collection bag 25 increases by a predetermined amount (for example, 30 g), the control unit 13 closes the first channel opening / closing unit 81 and sets the second channel opening / closing unit 82 to Release and move to constant-speed plasma circulation step.

And the above-mentioned constant-speed plasma circulation step,
A second plasma collection step and an accelerated plasma circulation step are performed, and the process proceeds to FIG. 19, where a small-volume plasma collection step for interface adjustment, a platelet collection step, and a blood return step are sequentially performed, and the final platelet collection operation is completed. I do. Final round and 2
The difference from the first time is that the bag in which air flows in the above-mentioned constant-speed plasma circulation step is different, and in the last time, the blood return step is performed without performing the buffy coat collection step.

In the final platelet collection step,
As shown in FIG. 19, at the time of the second platelet collection step, if the BC interface parameters are automatically rewritten, the correction conditions are used. Is performed. Also, after acceleration starts,
If the density change is appropriate, the process proceeds to [10] in FIG.
(If it is an abnormal pattern, move to [11] in FIG. 20)
The actual collection of platelets is started, and in this second platelet collection step, as in the first time, the determination of the platelet collection process pattern and the collection of platelets at the appropriate time for each pattern are terminated. Automatic rewriting of the BC interface parameters when recognized as a pattern is not performed.

Next, a specific description will be given of the platelet collection operation of the embodiment having a function of judging the state of platelet outflow using the data of the first platelet collection step at the time of the second and subsequent platelet collection station operations. An anticoagulant is added to whole blood at a predetermined ratio (1/8 to 1/20, specifically 1/10 with respect to whole blood) at a predetermined speed (250 ml / min or less; preferably 150 to 40 ml / min). min or less, specifically, 6
(0 ml / min or less) to the centrifuge 20 via the first line 21 to rotate the centrifuge 20 at a predetermined rotation speed (30
00 to 6000 rpm, preferably 4400 to 580
By rotating at 0 rpm, blood is separated into plasma, buffy coat, and red blood cell components. When the plasma overflows the centrifuge 20, it is collected in a plasma bag, and the plasma is collected in a predetermined amount (10 to 150 ml, preferably 20 to 150 ml). ~ 30ml)
At the time of collection, the blood supply is stopped, and the plasma is collected under a predetermined condition (at a speed higher than the blood collection volume, 60 to 250 ml / min).
And the first circulation is 200 m
At 1 / min × 30 sec), a constant-speed plasma circulation is performed by returning to the centrifugal separator 20 through the first line 21 and the second line 22.

Then, an anticoagulant is again added to whole blood (1/8 to 1/20, specifically 1/10 with respect to whole blood).
At a predetermined rate (250 ml / min or less; preferably, 150 to 40 ml / min or less, specifically,
(60 ml / min or less) to the centrifugal separator 20 via the first line 21 to rotate the centrifugal separator 20 at a predetermined rotation speed (3
000-6000 rpm, preferably 4700-48
00 rpm) to separate blood into plasma, buffy coat, and red blood cell components. When the blood cell interface position inside the centrifuge 20 is detected by the buffy coat interface detection sensor, the blood supply is stopped. Under predetermined conditions (initial speed
80 ml / min, final arrival speed (set speed) 150 to
250 ml / min, acceleration conditions (every second) 2-1
0 ml / min speed increase, circulation time 10 to 90 sec
In c), an accelerated plasma circulation is performed, returning to the centrifuge 20 through the first line 21 and the second line 22.

After the last blood collection has been performed, the plasma is passed through the first and second lines 22 under predetermined conditions.
And stepwisely increase the acceleration under predetermined conditions (stepwise acceleration; 0.1 to 99 ml / min / sec).
c, specifically, 2-10 ml / min / sec) to reach the platelet collection rate (60-250 ml / min),
The platelets that have flowed out from the centrifuge 20 are collected in a platelet collection bag 26. In particular, in the blood component collection device of the present invention, the platelet collection process can be performed based on the signal detected by the turbidity sensor 14 and the signal change pattern calculated from the signal, whether the stored normal pattern or a plurality of exceptional patterns. Is determined, and the platelet collection step ends at a time point suitable for the determined pattern.

Further, in this apparatus, after collecting the platelets, the blood circulation speed is maintained (60 to 250 ml / min, specifically, 200 ml / min), and the centrifuge 20
Lower the number of revolutions (100-30
By lowering the buffy coat by about 0 rpm), the buffy coat that has flowed out is collected, and the collected buffy coat is supplied to the centrifugal separator 20 before performing blood collection in the next cycle. The buffy coat may be collected by accelerating the blood circulation speed to a predetermined speed (equal to or higher than the platelet collection speed, preferably 60 to 250 ml / min, specifically 205 ml / min) after collecting the platelets. Good.

The blood component collection step (first platelet collection operation) by the blood component collection device 4 of this embodiment will be described with reference to the flowchart shown in FIG. In this example,
The platelet collection operation is repeated twice, and after the platelet collection step other than the last one is completed, before the blood return step, a buffy coat collection step is performed, and the centrifuge 20 is placed before the next blood collection step. A buffy court return step for returning this is performed. The seventh flow path opening / closing means 87 is closed, and the fourth line 24 for injecting fluid is not used.

First, the third line 23 and the blood collection needle 29 are primed with an anticoagulant, and then the donor is punctured with a puncture needle. Then, the centrifuge drive device 10 performs a first plasma collection step of rotating the rotor to collect a first predetermined amount of plasma from the blood into the plasma collection bag 25. When the first blood collection is started, the blood pump 11 starts collecting blood at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, simultaneously supplies the anticoagulant (for example, ACD-A liquid) at a predetermined speed (for example, 1/10 of the blood pump speed). The blood collected from the donor is mixed with the ACD-A solution, flows through the first line 21, and the chamber, the first channel opening / closing unit 8
1 and flows into the centrifuge 20. At this time, the sixth flow path opening / closing means 86, the fifth flow path opening / closing means 85,
Are closed, the first channel opening / closing unit 81 and the fourth channel opening / closing unit 8 are closed.
4 is open. When the ACD-A solution-added blood (ACD-added blood) is supplied to the centrifugal separator 20, the sterilized air that has entered the centrifugal separator 20 flows through the second line 22, and the fourth channel opening / closing means 84 After passing through, it flows into the platelet collection bag 26. Simultaneously with the start of the blood collection process, the centrifuge 20 starts rotating at a predetermined speed.
Since the supply of the CD blood is supplied, the blood is centrifuged in the separator, and the blood is separated from the inside into three layers, a plasma layer, a buffy coat layer (BC layer), and a red blood cell layer. Is supplied, the centrifuge 20 is completely filled with blood, and the plasma flows out from the outlet of the centrifuge 20. The turbidity sensor 14 attached to the second line 22 connected to the outlet of the centrifuge 20 detects that the fluid flowing in the line has changed from air to plasma,
Closes the fourth flow path opening / closing means 84 based on the detection signal of the turbidity sensor 14 and
Is released, and the plasma is collected in the plasma collection bag 25. The weight of the plasma collection bag 25 is
Is measured by the control unit 1
3 has been entered. Therefore, the weight of the plasma collected in the plasma collection bag 25 is the first predetermined amount (10 to 150 g,
For example, when it increases by 30 g), the control unit 13 closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to a constant speed plasma circulation step.

In the constant-velocity plasma circulation step, the blood collection is temporarily stopped, and the centrifuge driving device 10 is operated to remove the plasma collected in the plasma collection bag 25 to the centrifugal separator 20.
At a constant speed. When entering the constant-speed plasma circulation step, the control unit 13 maintains the closed state of the first flow path opening and closing means 81 and the open state of the second flow path opening and closing means 82,
The CD pump 12 stops, and the blood pump 11 operates at a predetermined speed (60 to 250 ml / min, for example, 200 ml / m).
In), the plasma in the plasma collection bag 25 is sent to the centrifugal separator 20 rotating at a predetermined speed through the second channel opening / closing means 82. At the same time, the plasma flowing out of the centrifuge 20 flows into the plasma collection bag 25 through the turbidity sensor 14 and the third channel opening / closing means 83. A predetermined time (10 to 90 seconds, e.g., 3
0 seconds), the control unit 13 closes the second flow path opening / closing means 82, opens the first flow path opening / closing means 81,
To the plasma collection step. The first plasma circulation is preferably performed at a flow rate of at least 60 ml / min or more for 10 seconds or more.

In the second plasma collection step, the first liquid supply pump 11 and the second liquid supply pump 12 are operated to collect the blood to which the anticoagulant has been added, and usually, the amount of plasma in the bag is reduced. When the optical sensor 15 detects the buffy coat layer of the separator due to the increase, this signal is sent to the control unit 13, and the control unit 13 closes the first flow path opening / closing means 81 and closes the second flow path. The road opening / closing means 82 is opened, and the process proceeds to the accelerated plasma circulation step. Specifically, the first liquid supply pump 11 starts collecting blood at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, is also simultaneously driven at a predetermined speed (eg, 1/1 of the blood pump speed).
At 0), an anticoagulant (for example, ACD-A solution) is supplied.
Blood collected from the donor is mixed with ACD-A solution,
The blood flows into the centrifugal separator 20 rotating at a predetermined speed, and the plasma is collected in the plasma collection bag 25. Usually, when the optical sensor 15 detects the buffy coat layer of the separator due to an increase in the amount of plasma in the bag, this signal is sent to the control unit 13, and the control unit 13 Is closed, the second flow path opening / closing means 82 is opened, and the process proceeds to the accelerated plasma circulation step. In the plasma collection step, plasma is collected until the sensor 15 detects a buffy coat (BC interface: interface between the plasma layer and the buffy coat layer). In the apparatus of this embodiment, FIGS.
As shown in the flowchart of FIG. 8, the detection of the BC interface is performed in each plasma collection step, and if the BC interface is detected during the first plasma collection step, the plasma collection is interrupted. Then, move to the accelerated plasma circulation step.

In the accelerated plasma circulation step, the blood collection is temporarily interrupted, and the centrifuge driving device 10 is operated to circulate the plasma in the plasma collection bag 25 to the centrifuge 20 while accelerating. At this time, the blood pump speed is lower than the constant speed plasma circulation step, for example, 60 ml / mi.
n and the final speed is 150-200 ml / mi
Accelerate until n is reached. The acceleration conditions are as follows: a speed of 2 to 10 ml / min increases every second, 200 m
It is performed with an arrival time of 1 / min about 14 to 70 seconds. After the end of the circulation step, the process proceeds to FIG. 12, and a small amount plasma collection step for interface adjustment is performed.

As shown in FIG. 12, in the step of collecting a small amount of plasma for adjusting the interface, in order to keep the position of the buffy coat layer in the platelet collecting step performed later irrespective of the donor, a predetermined amount of red blood cells is supplied. Collect blood. The red blood cell supply amount is defined as a value obtained by dividing the blood collection amount by the hematocrit value of the donor, and the blood collection amount is generally about 12 ml. Also in this blood collection, the first liquid supply pump 11 starts blood collection at a predetermined speed (for example, 60 ml / min). At this time,
The second pump, which is an anticoagulant pump, simultaneously supplies an anticoagulant (for example, ACD-A liquid) at a predetermined speed (for example, 1/10 of the blood pump speed). Blood collected from the donor is mixed with the ACD-A solution, flows into the centrifugal separator 20 rotating at a predetermined speed, and a small amount of plasma is collected. The control unit 13 calculates the collection time from the set collection amount and the pump speed, and ends the blood collection when the collection time has elapsed. Then, the control unit 13 closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to a platelet collection step. After the above steps are completed, the plasma circulation speed by the first liquid supply pump 11 is increased,
A platelet collection step is performed in which platelets are discharged from the centrifuge 20 and the platelets are collected in the platelet collection bag 26.
The platelet collection step is also called a so-called acceleration process. In this step, as shown in FIG. 12, initially, the blood pump speed is increased from 60 ml / min to 200 ml / mi.
2 ml / mi every predetermined time (for example, 1 second) until n
The control unit 13 operates the blood pump so as to accelerate by n. When the blood pump reaches 200 ml / min, the control unit 13 maintains the speed until the platelet collection step is completed.

When the platelet collection step is started, the turbidity sensor 14 detects the turbidity of the liquid passing therethrough, and the turbidity is output as a voltage value by the sensor.
3 is input. Blood pump speed increased to 120m
After reaching 1 / min, it is accelerated by 2 ml / min every predetermined time (for example, 1 second) until it is determined from the voltage value of the turbidity sensor that the PC concentration is 300,000 or more. When it is determined that the PC concentration is 300,000 or more,
The process moves to the one shown in FIG. The circulation speed is 200 ml /
If the PC concentration does not reach 300,000 or more even after a predetermined time (for example, 10 seconds) has elapsed after reaching min, platelet collection is terminated as an abnormal pattern, and the process proceeds to FIG. In addition, the circulation speed at the time of outflow of platelets is referred to as outflow speed. When platelet collection is started, platelet collection time measurement is also started. The turbidity of the liquid passing through the turbidity sensor 14 increases due to the outflow of platelets, and it is determined whether the PC concentration is 1,000,000 or more based on the voltage value output from the sensor. If the PC collection amount (platelet collection weight) has reached the set value, platelet collection is terminated at that time, and it is recognized that the exception pattern A has been met. Also, when the PC concentration reaches 1,000,000 or more from the voltage value output from the sensor, and then the PC concentration (sensor voltage value) reaches the RBC level (red blood cell detection voltage), at that time, End of platelet collection and exception pattern B
Recognize that In addition, the PC concentration reaches 1,000,000 or more from the voltage value output from the sensor, and thereafter, the PC collection amount (platelet collection weight) increases without the sensor voltage value reaching the lower limit peak (PC concentration is the highest value). When the set value is reached, platelet collection is terminated at that time, and it is recognized that the exception pattern C has been met. Also, from the voltage value output from the sensor, PC
When the concentration reaches 1,000,000 or more, and thereafter, the sensor voltage value reaches the lower limit peak (PC concentration is the highest value), the control unit determines the platelet outflow lower limit peak time (Tpeak1) and the platelet outflow lower limit peak voltage (Tpeak1). Vpeak1) is stored, and thereafter, the slope of the PC outflow curve (the slope of the sensor voltage value change) is continuously calculated. Thereafter, when the PC collection amount (platelet collection weight) reaches the set value, at that time, the platelet collection is terminated, and it is recognized that the exception pattern D has been met. If the PC collection amount (platelet collection weight) reaches the set value in a state where the slope of the PC outflow curve (slope of sensor voltage value change) is continuously calculated, the pattern falls under the normal pattern. At the same time, the platelet collection is terminated at that time. In a state where the slope of the PC outflow curve (the slope of the sensor voltage value change) is continuously calculated,
The PC outflow curve (slope of sensor voltage value change) is “+” without the PC collection amount (platelet collection weight) reaching the set value and the PC voltage (sensor voltage value) also not reaching the end voltage. "" To "0", the platelet collection is terminated at that time, and it is recognized that the exception pattern E has been met.

In any of the above cases, the third passage opening / closing means 83 is closed and the fourth passage opening / closing means 84 is closed.
Opens, and the platelet-rich plasma flowing out of the centrifuge 20 is collected in the platelet collection bag 26. Further, the voltage value output from the turbidity sensor 14 is converted into a platelet concentration by the control unit 13, and the platelet concentration in the platelet collection bag 26 during platelet collection is calculated.

When the platelet collection is completed, the process proceeds to the buffy coat collection step, and the process also proceeds to FIG. 14, where post-processing according to the corresponding pattern is performed in the platelet collection process. When proceeding to the post-processing decision, it is analyzed which of the platelet collection process patterns corresponded,
In the case other than the normal pattern, the next BC interface change condition is determined. Specifically, in the case of exception pattern A,
Reduce the BC interface detection setting by 2%. In the case of the exceptional pattern B, the detection setting of the BC interface is increased by 2%. In the exceptional patterns C and D, when the signal voltage (final voltage) of the turbidity sensor at the time of platelet collection is 2 V or more, the voltage is lowered by 2%, and when it is 1-2 V, it is lowered by 3%.
In the case of the exceptional pattern E, the circulation speed (outflow speed) is
If it is less than a predetermined value (for example, 130 ml / min),
2% increase, if it is within a predetermined range (for example, from 130 ml / min to less than 200 ml / min), increase by 1%, and if the outflow speed is 200 ml / min or more, do not change the BC interface detection setting. And Then, the BC interface parameters are automatically rewritten according to the above change conditions.

In the buffy coat collection step, when the above-described platelet collection step is completed, the control unit 13
Is closed, and the fifth channel opening / closing means 85 is opened. The blood plasma in the plasma collection bag 25 is sent to the centrifugal separator 20 by the blood pump 11, and the liquid (the buffy coat layer has flowed out) from the centrifuge 20 simultaneously flows into the buffy coat collection bag 27. .
In the buffy coat collecting step, the speed of the blood pump 11 is maintained at the final speed in the platelet collecting step, and the centrifugal separator 20 is rotated at a predetermined speed or a rotation speed slightly higher than the predetermined speed (50 to more than the predetermined speed). 2
The rotation speed is increased to about 00 rpm (for example, a rotation speed higher by 100 rpm), whereby the buffy coat flows out from the centrifuge 20 and is collected in the buffy coat collection bag 27. When the amount calculated from the donor's hematocrit value and the amount of collected platelets is collected, the blood pump 11 stops, all valves close, the rotation of the centrifuge 20 stops, and the buffy coat collection step ends.

Next, a blood return step of returning the blood in the centrifuge 20 is performed. The control unit 13 rotates the blood pump 11 in the reverse direction, opens the first channel opening / closing unit 81,
The red blood cell layer remaining in the centrifuge 20 is transferred to the first line 2
Blood is returned to the donor from 1. This is the first time (first time)
When the platelet collection operation is completed, the process proceeds to the step shown in FIG. 15, and a second platelet collection operation is performed. First, as shown in FIG. 15, a buffy coat return step of returning the buffy coat collected by the first platelet collection operation into the centrifuge 20 before the next plasma collection step is performed. When the process proceeds to the buffy coat return step, the control unit 13 rotates the centrifugal separator 20 at a predetermined rotation speed (calculated value of the initial rotor rotation speed), and the fifth flow passage opening / closing device 85 and the fourth flow passage opening / closing device 84 Is opened, and the blood pump 11 is set to a predetermined speed (the default is 100 ml / mi).
Operate in n). The buffy coat contained in the buffy coat collection bag 27 is supplied to the centrifugal separator 20 through the fifth channel opening / closing means 85. The air in the centrifuge 20 is sent to the platelet collection bag 26 through the second line 22 and the fourth channel opening / closing means 84. After the blood pump 11 rotates by the amount of the buffy coat collected, the buffy coat return step ends.

Then, the flow shifts to that shown in FIG. 16, and a small amount of plasma collection step, a platelet collection step, a buffy coat collection step, and a blood return step are sequentially performed, and the second platelet collection operation is completed. In this platelet collection step, as shown in FIG. 16, in the first platelet collection step, if the BC interface parameters are automatically rewritten, the correction condition is not used. In the method, platelets are collected under the initial conditions. In addition, after the acceleration starts, if the density change is appropriate, the process proceeds to FIG. 22.
Then, when the actual collection of platelets is started,
In the second platelet collection step, the platelet collection process pattern is determined, the platelet collection is terminated at a time suitable for each pattern, and the BC interface parameters are automatically rewritten when the pattern is recognized as an exception pattern. Will be

Further, in the second platelet collection step, as shown in FIG. 22, the measurement of the platelet collection time (T) is started at the same time as the start of platelet collection, and while the turbidity sensor does not detect the lower limit peak, the control unit operates. It is determined whether the platelet collection time from the start of platelet collection has reached the platelet outflow lower limit peak time (Tpeak1) stored in the control unit. If it has arrived, the flow shifts to [12] in FIG. 23, and it is determined whether or not it corresponds to the special platelet collection pattern. First, the platelet outflow lower limit peak time (Tpeak
1) The turbidity sensor voltage (V2) at the time of arrival is stored, and it is determined again whether the turbidity sensor voltage has reached the lower limit peak (PC concentration is the highest value). When the lower limit peak is detected, the process shifts to [13] in FIG. 22 to escape from the special pattern determination program and return to the normal program. If the lower limit peak is not detected, the turbidity sensor voltage (V2) becomes the peak voltage (Vp
eek1) + a predetermined value (specifically, 0.5v). Specifically, it is determined whether V2−Vpeek1 is greater than a predetermined value (permissible value). When the difference is larger than the predetermined value, it is determined that “there is an abnormal tendency”, and the process proceeds to an end determination program.

In the end determination program, the turbidity sensor voltage (V) after a lapse of a predetermined time (specifically, about 2 seconds)
2a) and the turbidity sensor voltage change rate during a predetermined time (2 seconds) is calculated from the turbidity sensor voltage (V2) at the time when the first peak time (Tpeek1) is reached, and this turbidity sensor voltage change rate is the first rate. Is determined to be larger than the allowable value (specifically, -0.2v). If the turbidity sensor voltage change rate is equal to or less than the first allowable value, it is determined that the turbidity sensor is abnormal, and the platelet collection step is terminated. When the turbidity sensor voltage change rate is larger than the first allowable value, the turbidity sensor voltage (V2b) after a lapse of a predetermined time (specifically, about 2 seconds) and the turbidity sensor voltage From (V2a), the turbidity sensor voltage change rate for a predetermined time (predetermined second) is calculated, and it is determined whether or not this turbidity sensor voltage change rate is greater than a second allowable value (for example, -0.1v). If the rate of change of the turbidity sensor voltage is equal to or less than the second allowable value, it is determined to be abnormal and the platelet collection step is terminated.
If the turbidity sensor voltage change rate is larger than the second allowable value, the process exits from the special pattern determination program, returns to the normal program, and continues the platelet collection step.

Next, the final platelet collection operation shown in FIG. 18 will be described. In this embodiment, the third time is the final time, but the present invention is not limited to this, and the fourth and subsequent times may be the final time of the platelet collection operation. In this case, except for the last round, it is the same as the second platelet collection operation (FIGS. 16 and 22). First, as shown in FIG. 18, the buffy coat collected by the second platelet collection operation was centrifuged into a centrifuge 20 before the next plasma collection step.
Perform a buffy coat return step to return within.

At the buffy coat return step, the controller 13 rotates the centrifuge 20 at a predetermined rotation speed (for example, 4800 rpm), and the fifth flow path opening / closing means 85 and the fourth flow path opening / closing means 84 Open the blood pump 1
1 is operated at a predetermined speed (the default is 100 ml / min). The buffy coat contained in the buffy coat collection bag 27 is supplied to the centrifugal separator 20 through the fifth channel opening / closing means 85. The air in the centrifuge 20
It is sent to the platelet collection bag 26 through the second line 22 and the fourth channel opening / closing means 84. After the blood pump 11 rotates by the amount of the buffy coat collected, the buffy coat return step ends.

Next, the first liquid feed pump 11 and the second liquid feed pump 12 are operated to collect the blood to which the anticoagulant has been added, and the centrifuge driving device 10 is operated to remove the blood. A first plasma collection step of collecting a first predetermined amount of plasma in the plasma collection bag 25 is performed. When the first blood collection is started, the first liquid supply pump 11 operates at a predetermined speed (for example, 60
(ml / min). At this time, the second pump, which is an anticoagulant pump, also has a predetermined speed (for example,
Anticoagulant (eg, AC at 1/10 of the blood pump speed)
(DA solution). The blood collected from the donor is A
The mixture is mixed with the CD solution, flows through the first line 21, passes through the chamber, the first channel opening / closing means 81, and
Flows into. At this time, the sixth channel opening / closing means 86 and the fifth
, The second channel opening / closing unit 82, the third channel opening / closing unit 83, and the seventh channel opening / closing unit 87 are closed, and the first channel opening / closing unit 81 and the fifth channel opening / closing unit 87 are closed. Channel opening / closing means 85
Is open, and when the ACD blood is supplied to the centrifuge 20, the sterilized air originally contained in the centrifuge 20 passes through the line sensor, the fifth flow path opening / closing means 85, and the buffy coat collection bag 27. Flows into. Simultaneously with the start of the blood collection process, the centrifugal separator 20 starts rotating at a predetermined speed (if the corrected rotor rotational speed has been calculated, if it has not been calculated, otherwise the initial rotor rotational speed calculated value). 20 receives the supply of ACD blood while rotating, the blood is centrifuged in the separator, and the blood is separated from the inside into three layers, a plasma layer, a buffy coat layer (BC layer), and a red blood cell layer. ACD blood over the capacity of the separator (about 2
70 ml), the inside of the centrifuge 20 is completely filled with blood, and the plasma flows out from the outlet of the centrifuge 20. The second connected to the outlet of the centrifuge 20
The turbidity sensor 14 attached to the line 22 detects that the fluid flowing in the line has changed from air to plasma, and the control unit 13 performs the fifth operation based on the detection signal of the turbidity sensor 14. The flow path opening / closing means 85 is closed, and the third
Is opened, and the plasma is collected in the plasma collection bag 25. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. Therefore, when the weight of the plasma collected in the plasma collection bag 25 increases by a predetermined amount (for example, 30 g), the control unit 13 closes the first channel opening / closing unit 81 and sets the second channel opening / closing unit 82 to Release and move to constant-speed plasma circulation step.

And the above-mentioned constant-speed plasma circulation step,
A second plasma collection step and an accelerated plasma circulation step are performed, and the process proceeds to FIG. 19, where a small-volume plasma collection step for interface adjustment, a platelet collection step, and a blood return step are sequentially performed, and the final platelet collection operation is completed. I do. Final round and 2
The difference from the first time is that the bag in which air flows in the above-mentioned constant-speed plasma circulation step is different, and in the last time, the blood return step is performed without performing the buffy coat collection step.

In the final platelet collection step,
As shown in FIG. 19, at the time of the second platelet collection step, if the BC interface parameters are automatically rewritten, the correction conditions are used. Is performed. Also, after acceleration starts,
If the density change is appropriate, the process proceeds to [10] in FIG.
(In the case of an abnormal pattern, shift to [11] in FIG. 24)
The actual collection of platelets is started, and in the final platelet collection step, as in the first time, the determination of the platelet collection process pattern and the collection of platelets at the appropriate time for each pattern are terminated. Automatic rewriting of the BC interface parameters when recognized as a pattern is not performed. In the final platelet collection step, as in the first time, as shown in FIG. 24, measurement of the platelet collection time (T) is started at the same time as the start of platelet collection, and the measurement is performed while the turbidity sensor does not detect the lower limit peak. The control unit determines whether the platelet collection time from the start of platelet collection has reached the platelet outflow lower limit peak time (Tpeak1) stored in the control unit. If it has arrived, the flow shifts to [14] in FIG. 25, and it is determined whether or not it corresponds to the special platelet collection pattern. First, the turbidity sensor voltage (V) when the platelet outflow lower limit peak time (Tpeak1) is reached.
n) is stored, and it is determined again whether the turbidity sensor voltage has reached the lower limit peak (PC concentration is the highest value). When the lower limit peak is detected, the flow shifts to [15] in FIG. 24 to escape from the special pattern determination program and return to the normal program. If the lower limit peak is not detected, whether the turbidity sensor voltage (Vn) is larger than the peak voltage (Vpeek1) in the first platelet collection step + a predetermined value (specifically, 0.5 V) Compare. Specifically, it is compared whether Vn-Vpeek1 is greater than a predetermined value (allowable value). When the difference is larger than the predetermined value, it is determined that “there is an abnormal tendency”, and the process proceeds to an end determination program.

In the end determination program, the turbidity sensor voltage (V) after a certain time has elapsed (specifically, about 2 seconds)
na) and the turbidity sensor voltage (Vn) at the time when the first peak time (Tpeek1) is reached, the turbidity sensor voltage change rate in a predetermined time (2 seconds) is calculated. Is determined to be larger than the allowable value (specifically, −0.2 V). If the turbidity sensor voltage change rate is equal to or less than the first allowable value, it is determined that the turbidity sensor is abnormal, and the platelet collection step is terminated. If the turbidity sensor voltage change rate is larger than the first allowable value, the turbidity sensor voltage (V2n) after a lapse of a predetermined time (specifically, about 2 seconds) and the turbidity sensor voltage From (Vna), the turbidity sensor voltage change rate for a predetermined time (predetermined second) is calculated, and it is determined whether the turbidity sensor voltage change rate is greater than a second allowable value (for example, -0.1v). If the rate of change of the turbidity sensor voltage is equal to or less than the second allowable value, it is determined to be abnormal and the platelet collection step is terminated.
If the turbidity sensor voltage change rate is larger than the second allowable value, the process exits from the special pattern determination program, returns to the normal program, and continues the platelet collection step.

[0097]

(Embodiment 1) With a circuit configuration as shown in FIG.
An apparatus composed of two ACD pumps and a blood pump was prepared, and the platelet collection performance was compared. Comparative experiments were performed using the same donor at intervals of 2 weeks or more. A comparison of the operation flows evaluated below is shown. n =
Comparison was made in two cases. Platelet count of the obtained concentrated platelet plasma,
Leukocyte counts were measured on a Sysmex® NE-6000. However, the lower limit of WBC measurement of Sysmex is 0.1 × 1.
Since it is 0 ² [Cells / muL], the sample below the lower limit of measurement was measured using the Agegotte [1: 9] method. The amount of platelets collected was 40 ml. The platelet collection operation is performed three times. In the platelet collection step, the above-described normal pattern and the exceptional patterns A to E are determined, the platelet collection ends at a time suitable for each pattern, and the BC interface when the exceptional pattern is recognized. Automatic rewriting of parameters was performed. In the case of the exception pattern A, the detection setting of the BC interface is lowered by 2%, in the case of the exception pattern B, the detection setting of the BC interface is raised by 2%, and in the case of the exception pattern C and the exception pattern D, the platelet If the signal voltage (final voltage) of the turbidity sensor at the time of sampling is 2 V or more, lower by 2%; if it is 1 to 2 V, lower by 3%; Is increased by 2%. In the case of exceptional pattern E, the circulation speed
Is less than or equal to a predetermined value (for example, 130 ml / min), it is increased by 2% and is within a predetermined range (for example, 130 ml / mi).
n less than 200 ml / min), increase 1%,
When the outflow speed is 200 ml / min or more, BC
The interface detection setting was not changed.

The blood collection rate was 40 ml / min and the first circulation condition (200
ml / min, 30 sec), the second circulation condition (initial speed 60 ml / min, arrival speed 170 m) performed after detection of the BC interface
1 / min, acceleration conditions 5 ml / min / sec, acceleration time 22 sec), acceleration conditions during platelet collection (10 ml / min)
min / sec), platelet collection speed (200 ml / mi)
n, to a predetermined concentration of platelet collection), the buffy coat collection speed (195 ml / min, 30 ml), the buffy coat was returned to the centrifuge before blood collection for the second and third times, and the buffy coat was collected for the third time. Did not do. The centrifugal rotation speed was 4800 rpm (Hct = 35%).

(Comparative Example 1) As an apparatus, a normal pattern and an exceptional pattern A to
The procedure was the same as in the example except that the determination of E, the termination of platelet collection at a time suitable for each pattern, and the automatic rewriting of the BC interface parameters when an exceptional pattern was recognized were not performed. The platelet collection operation was performed three times, the blood collection rate was 40 ml / min, and the first circulation condition (200 ml / min, 30 sec) performed after the total amount of plasma was 30 g.
c), the second circulation condition (initial speed 60 m
1 / min, arrival speed 170 ml / min, acceleration condition 5
ml / min / sec, acceleration time 22 sec), acceleration conditions during platelet collection (10 ml / min / sec), platelet collection speed (200 ml / min, up to a predetermined concentration of platelet collection), buffy coat collection speed (195 ml / mi)
n, 30 ml), the buffy coat was returned to the centrifuge before blood collection for the second and third times, and the buffy coat was not collected for the third time. Centrifugal rotation speed is 480
It was set to 0 rpm (Hct = 35%). And the platelet concentration of the concentrated platelet plasma of the collected Example and Comparative Example,
Leukocyte concentration and blood processing volume are as follows.

[0100]

[Table 1]

[0101]

[Table 2]

[0102]

[Table 3]

(Example 2) An apparatus composed of two ACD pumps and a blood pump having the circuit configuration shown in Fig. 4 was manufactured, and the platelet collection performance was compared. Comparative experiments were performed using the same donor at intervals of 2 weeks or more. A comparison of the operation flows evaluated below is shown. n = 2 cases were compared. The platelet count and leukocyte count of the obtained concentrated platelet plasma were determined by Sysmex® NE-6000.
Was measured. However, since the lower limit of the WBC measurement of Sysmex is 0.1 × 10 ² [Cells / muL], the sample below the lower limit of the measurement has Nageotte [1: 9].
It was measured using the method. The amount of platelets collected was 40 ml.

The platelet collection operation is performed three times. In the platelet collection step, the above-described normal pattern and exceptional patterns A to E are determined, the end of platelet collection at a time suitable for each pattern, and when an exceptional pattern is recognized. Of B
Automatic rewriting of the C interface parameter was performed. In addition,
In the case of the exceptional pattern A, the detection setting of the BC interface is set to 2%.
In the case of exception pattern B, the detection setting of the BC interface is increased by 2%. In the case of the exceptional pattern E, it is lowered by 2%, in the case of 1-2 V, it is lowered by 3%.
The interface detection setting is increased by 2%, and in the case of the exceptional pattern E, the circulation speed (outflow speed) is set to a predetermined value (for example, 130
ml / min) or less, increase by 2% and within a predetermined range (for example, 130 ml / min to 200 ml / min).
), The outflow rate is increased by 200% /
In the case of not less than min, the detection setting change of the BC interface is not performed.

When the PC concentration reaches 1,000,000 or more,
Thereafter, when the sensor voltage value reaches the lower limit peak (PC concentration is the highest value), the control unit stores the platelet outflow lower limit peak time (Tpeak1) and the platelet outflow lower limit peak voltage (Vpeak1). Then, in the second and subsequent platelet collection steps, measurement of the platelet collection time (T) is started at the same time as the start of platelet collection. The turbidity sensor voltage (V2) is stored, and the turbidity sensor voltage (V2) is equal to the peak voltage (Vpeek1) +0.5.
When the value is larger than v, the process shifts to the special termination determination program. Then, in the exceptional termination judgment program, 2
If the rate of change of the turbidity sensor voltage calculated from the turbidity sensor voltage (V2a) and the turbidity sensor voltage (V2) after -seconds is -0.2 V or less, it is determined that there is an abnormality, and the platelet collection step is performed. It is to be terminated. Further, when the turbidity sensor voltage change rate is larger than -0.2 v,
If the rate of change of the turbidity sensor voltage calculated from the turbidity sensor voltage (V2b) and the turbidity sensor voltage (V2a) two seconds later is -0.1 V or less, it is determined that there is an abnormality, and the platelet collection is performed. The steps are to be terminated.

The blood collection rate was 40 ml / min and the first circulation condition (200
ml / min, 30 sec), the second circulation condition (initial speed 60 ml / min, arrival speed 170 m) performed after detection of the BC interface
1 / min, acceleration conditions 5 ml / min / sec, acceleration time 22 sec), acceleration conditions during platelet collection (10 ml / min)
min / sec), platelet collection speed (200 ml / mi)
n, to a predetermined concentration of platelet collection), the buffy coat collection speed (195 ml / min, 30 ml), the buffy coat was returned to the centrifuge before blood collection for the second and third times, and the buffy coat was collected for the third time. Did not do. The centrifugal rotation speed was 4800 rpm (Hct = 35%).

(Comparative Example 2) As the apparatus, the normal pattern and the exceptional patterns A to
E other than the determination of E, the termination of platelet collection at a time suitable for each pattern, and the automatic rewriting of BC interface parameters when recognized as an exceptional pattern, and the absence of the special exception termination determination program described above Was the same as in the example. The platelet collection operation was performed three times, the blood collection rate was 40 ml / min, and the first circulation condition (200 ml / min, 30 sec) performed after the total amount of plasma was 30 g.
c), the second circulation condition (initial speed 60 m
1 / min, arrival speed 170 ml / min, acceleration condition 5
ml / min / sec, acceleration time 22 sec), acceleration conditions during platelet collection (10 ml / min / sec), platelet collection speed (200 ml / min, up to a predetermined concentration of platelet collection), buffy coat collection speed (195 ml / mi)
n, 30 ml), the buffy coat was returned to the centrifuge before blood collection for the second and third times, and the buffy coat was not collected for the third time. Centrifugal rotation speed is 480
It was set to 0 rpm (Hct = 35%). And the platelet concentration of the concentrated platelet plasma of the collected Example and Comparative Example,
Leukocyte concentration and blood processing volume are as follows.

[0108]

[Table 4]

[0109]

[Table 5]

[0110]

[Table 6]

[0111]

According to the blood component collecting apparatus of the present invention, at the time of collecting platelets, the platelet collecting process is stored using the signal detected by the turbidity sensor and the signal change pattern calculated from the signal. A platelet collection process pattern determination function for determining whether the pattern is a normal pattern or a plurality of exceptional patterns, and a platelet collection step for ending the platelet collection step at a time suitable for the pattern determined by the platelet collection process pattern determination function An adjustment function at the end of the step is provided. Therefore, it is possible to obtain good platelets containing very little white blood cells and red blood cells.

Further, when the blood component collecting apparatus determines by the above-mentioned platelet collecting process pattern determining function that it is one of a plurality of exceptional patterns, it determines the platelet collecting condition in the next platelet collecting step. A platelet-containing liquid having a platelet collection condition adjustment function of adjusting platelet collection conditions to conditions suitable for the exceptional pattern, having very low leukocyte and red blood cell contamination and having a high platelet collection efficiency ( Concentrated platelet plasma). Further, in the platelet collection device of the present invention,
In the platelet collection step at the time of the second and subsequent plasma collection operations, using the data on the first platelet outflow,
Since a function is provided for judging the situation in the second and subsequent platelet collection steps and terminating the platelet collection step in the case of an abnormal state, platelets with less blood cell contamination can be collected.

[Brief description of the drawings]

FIG. 1 is a plan view showing a configuration example of a blood component collection circuit used in a blood component collection device of the present invention.

FIG. 2 is a plan view of a cassette housing part of the blood component collection circuit of FIG. 1;

FIG. 3 is a partially broken cross-sectional view showing a state in which a driving device is mounted on a centrifuge used in a blood component collection circuit.

FIG. 4 is a conceptual diagram of one embodiment of a blood component collecting apparatus of the present invention in a state where a blood component collecting circuit is mounted.

FIG. 5 is a diagram showing a normal change pattern of a signal detected by a turbidity sensor during platelet collection.

FIG. 6 is a diagram showing an example (exception pattern A) of an exception pattern of a signal detected by a turbidity sensor at the time of collecting platelets.

FIG. 7 is a diagram showing another example (exception pattern B) of an exception pattern of a signal detected by a turbidity sensor at the time of collecting platelets.

FIG. 8 is a diagram illustrating another example (exception pattern C) of an exception pattern of a signal detected by a turbidity sensor during platelet collection.

FIG. 9 is a diagram illustrating another example (exception pattern D) of an exception pattern of a signal detected by a turbidity sensor during platelet collection.

FIG. 10 is a diagram showing another example (exception pattern E) of an exception pattern of a signal detected by the turbidity sensor at the time of collecting platelets.

FIG. 11 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 12 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 13 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 14 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 15 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 16 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 17 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 18 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 19 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 20 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 21 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 22 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 23 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 24 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 25 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

[Explanation of symbols]

 Reference Signs List 1 blood component collecting device 2 blood component collecting circuit 10 centrifuge driving device 11 first liquid feed pump 12 second liquid feed pump 13 control unit 14 turbidity sensor 15 optical sensor 16 weight sensor 20 centrifuge 21 1 line 22 2nd line 23 3rd line 24 4th line 25 Plasma collection bag 26 Platelet collection bag 29 Blood collection needle 100 Blood component collection device

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) // B04B 1/04 B04B 11/04 11/04 13/00 13/00 A61J 1/00 331A

Claims (12)

[Claims] 1. A blood component collection device for separating blood into a plurality of blood components by a centrifugal separator having a blood storage space therein, and collecting a specific blood component into a bag. A first line for connecting the blood collection means and the inlet of the centrifuge, a second line connected to the outlet of the centrifuge, and a middle of the first line. A blood sample collection circuit comprising a plasma collection bag having a first tube and a second tube connected to the second line, and a platelet collection bag connected to the second line; A platelet collection is performed by using a turbidity sensor attached to an outlet or the second line and a signal detected by the turbidity sensor and a signal change pattern calculated from the signal during platelet collection. A platelet collection process pattern determination function for determining whether the process is a stored normal pattern or a plurality of exceptional patterns, and a platelet collection step at a time suitable for the pattern determined by the platelet collection process pattern determination function. A blood component collection device, comprising a platelet collection step end adjustment function for terminating the process. 2. The platelet collection process pattern determination function uses a signal detected by the turbidity sensor, a signal change pattern calculated from the signal, and a circulation speed at the time of platelet collection in the platelet collection step. 2. The blood component collection device according to claim 1, wherein the platelet collection process determines whether the stored pattern is one of a stored normal pattern and a plurality of exceptional patterns. 3. The blood component collection device determines a platelet collection condition in a next platelet collection step when the platelet collection process pattern determination function determines that the pattern is one of a plurality of exceptional patterns. 3. The blood component collection device according to claim 1, further comprising a platelet collection condition adjusting function for adjusting the platelet collection condition to a condition suitable for the selected exception pattern. 4. The platelet collection condition adjusting function adjusts the platelet collection condition such that a change pattern of a signal detected by the turbidity sensor at the time of the platelet collection step approaches a stored normal pattern. The blood component collection device according to claim 3. 5. The blood component collection device according to claim 1, further comprising: a centrifugal separator driving device for rotating a rotor of the centrifugal separator; and a centrifugal separator side from a connection between the first line and the first tube. A first liquid supply pump for the first line, a second liquid supply pump for the third line, and a plurality of valves for opening and closing the flow path of the blood component collection circuit. And a control unit for controlling the centrifugal separator driving device, the first liquid feed pump, the second liquid feed pump, and the plurality of flow path open / close means. Collecting a blood to which an anticoagulant has been added, separating the collected blood and collecting the separated plasma in the plasma collection bag, and the plasma collection bag collected by the plasma collection step Plasma in the centrifuge At least one plasma collecting and circulating step comprising a plasma circulating step to be circulated, and after the plasma collecting and circulating step, by accelerating the plasma circulating speed by the first liquid sending pump, A platelet collection operation in which a platelet collection step of causing platelets to flow out and collecting the platelets in the platelet collection bag is performed, and after the platelet collection step is completed, a blood return step of returning blood in the centrifuge is performed. Controlling the centrifuge drive device, the first liquid feed pump, the second liquid feed pump, and the plurality of flow path opening / closing means, and determining the platelet collection process pattern. The blood component collection device according to any one of claims 1 to 4, further comprising a function and an adjustment function at the end of platelet collection. 6. The plasma collection apparatus according to claim 1, wherein said platelet collection apparatus collects blood to which an anticoagulant is added, separates the collected blood, and collects the separated plasma in the plasma collection bag. Circulating the plasma in the plasma collection bag collected by the step to the centrifugal separator.
A circulating step and, after the plasma collecting and circulating step, a platelet for accelerating a plasma circulating speed by the first liquid supply pump, causing platelets to flow out of the centrifuge and collecting platelets into the platelet collecting bag. A function of controlling a platelet collection operation to be performed at least twice to perform a collection step and to perform a blood return step of returning blood in the centrifuge after the platelet collection step is completed. 6. The platelet collection device according to any one of 1 to 5. 7. The platelet collection process pattern judging function uses a signal detected by the turbidity sensor, a signal change pattern calculated from the signal, and a circulation speed during platelet collection in the platelet collection step. Determining whether the platelet collection process is a stored normal pattern or a plurality of exceptional patterns, and further, the plasma collection device determines that the first platelet collection process is a normal pattern. A first platelet collection process data storage unit that stores data related to the first platelet collection process, and a data comparison function for comparing data in the platelet collection process in the next and subsequent platelet collection steps with the first platelet collection process data. Having the adjustment function at the end of the platelet collection step,
The blood component collection according to claim 6, further comprising a function of terminating the platelet collection step when a difference of a predetermined value or more is detected between the initial platelet collection process data and the comparison target platelet collection process data by the data comparison function. apparatus. 8. The blood component collection device has a warning function that is activated when a difference of a predetermined value or more is detected between the initial platelet collection process data and the comparison target platelet collection process data by the data comparison function. Item 8. A blood component collecting apparatus according to Item 7. 9. The control unit has a function of measuring and storing a peak time from a start of the platelet collection step to a detection of a lower limit peak voltage by the turbidity sensor during a first platelet collection operation, and a storage function of a lower limit peak voltage. A comparison function for comparing whether the difference between the turbidity sensor voltage and the lower limit peak voltage at the time of the peak time from the start of the platelet collection step during the second or subsequent platelet collection operation is greater than a predetermined value, The adjustment function at the end of the platelet collection step is characterized in that the difference between the turbidity sensor voltage and the lower limit peak voltage when the peak time has been reached from the start of the platelet collection step in the second and subsequent platelet collection operations by the comparison function. When it is determined that the turbidity sensor voltage change rate for a predetermined second when it is determined that it is larger than a predetermined value, 9. The blood component collection device according to claim 6, wherein the platelet collection step is terminated when the calculated voltage change rate is smaller than a predetermined value. 10. The adjusting function at the end of the platelet collection step of the control unit, the voltage changing rate calculating function is performed again when the voltage changing rate in the predetermined second calculated by the voltage changing rate calculating function is larger than the predetermined value. 10. The blood component collection device according to claim 9, wherein a second voltage change rate in a predetermined second is calculated according to: and the platelet collection step is terminated when the calculated second voltage change rate is smaller than a predetermined value. 11. A blood component collecting device for separating blood into a plurality of blood components by a centrifugal separator having a blood storage space therein and collecting a specific blood component into a bag. A first line for connecting the blood collection means and the inlet of the centrifuge, a second line connected to the outlet of the centrifuge, and a middle of the first line. A blood sample collection circuit comprising a plasma collection bag having a first tube connected to the second tube and a second tube connected to the second line; a blood component collection circuit including a platelet collection bag connected to the second line; A turbidity sensor attached to an outlet or the second line, a centrifugal separator driving device for rotating a rotor of the centrifuge, and a control unit, wherein the control unit is added with an anticoagulant; Blood Collecting, separating the collected blood and collecting the separated plasma in the plasma collection bag, and circulating the plasma in the plasma collection bag collected in the plasma collection step to the centrifuge. A plasma circulation step comprising at least one plasma collection / circulation step; and, after the plasma collection / circulation step, by accelerating the plasma circulation speed by the first liquid supply pump to allow platelets from the centrifuge. A platelet collection step of causing the blood to flow out and collecting platelets into the platelet collection bag, and performing a blood return step of returning blood in the centrifuge after the platelet collection step is completed. The platelet collection step during the first platelet collection operation. Measurement and storage of the peak time from the start of the plateau to the detection of the lower limit peak by the turbidity sensor, and the storage function of the lower limit peak voltage, and from the start of the platelet collection step at the time of the second and subsequent platelet collection operations to the time at which the peak time has been reached. A comparison function for comparing whether the difference between the turbidity sensor voltage and the lower limit peak voltage is larger than a predetermined value, and the peak time from the start of the platelet collection step in the second and subsequent platelet collection operations by the comparison function. A voltage change rate calculating function for calculating a turbidity sensor voltage change rate for a predetermined second when it is determined that a difference between the turbidity sensor voltage and the lower limit peak voltage at the time is greater than a predetermined value; When the voltage change rate calculated by the rate calculation function is smaller than a predetermined value, the platelet collection step is terminated. Blood component collection apparatus characterized by comprising end adjustment function platelet collection step. 12. The adjustment function at the end of the platelet collection step of the control unit, wherein the voltage change rate calculation function is performed again when the voltage change rate in the predetermined second calculated by the voltage change rate calculation function is larger than the predetermined value. 12. The blood component collection device according to claim 11, wherein a second voltage change rate in a predetermined second is calculated according to: and the platelet collection step is terminated when the calculated second voltage change rate is smaller than a predetermined value.