JP2002126073A - Platelet collection device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a platelet collection device which makes it possible to accurately circulate plasma with a target circulation volume, thus reducing the mixture of white blood cell and red blood cell in the platelet to be collected and retraining a higher efficiency of platelet collection. SOLUTION: The platelet collection device 1 is equipped with the functions to calculate a unit working volume and a volume of blood to be delivered by a blood sending pump 11 for computing an arithmetic volume of blood to be sent per unit working volume of the blood sending pump 11, making use of the working volume of the blood sending pump 11 from the start of sending blood to filling a centrifugal separator 20 with the blood that contains an anticoagulant and a rotating volume of the centrifugal separator 20, and to control the volume by the blood sending pump 11, making use of the arithmetic volume of blood to be sent by computing the unit working volume of the blood sending pump 11 in a step to collect the platelets in a platelet collection bag 26, where the platelet is allowed to flow from the centrifugal separator 20 by accelerating the plasma circulation velocity at least in the operational procedure of collecting platelets by the blood sending pump 11.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a platelet collecting apparatus for collecting platelets from blood.

[0002]

2. Description of the Related Art At the time of blood collection, at present, blood is separated into various blood components by centrifugation or the like for reasons such as effective use of blood and reduction of the burden on donors, and only components necessary for the transfuser are collected. Blood is collected from components collected and returned to donors. In such a component blood sampling, when obtaining a platelet preparation, blood collected from a donor is introduced into a platelet collection circuit, and plasma, leukocytes, platelets, and blood are collected by a centrifuge called a centrifuge bowl installed in the platelet collection circuit. Separated into four components of red blood cells, platelets are collected in a container, platelet preparations and plasma are also collected in separate containers and used as raw materials for plasma preparations or plasma fraction preparations, and the remaining white blood cells and red blood cells are supplied to the donor. A blood return is performed.

As a platelet collection device, for example, Japanese Patent Application Laid-Open
There is one disclosed in Japanese Patent Publication No. 509403. In the apparatus disclosed in Japanese Patent Application Laid-Open No. Hei 8-509403, whole blood is fed into a centrifugal bowl, centrifuged to separate into a plasma component, a platelet component, and a blood cell component. A collection step is performed. In the platelet collection step, while maintaining the rotation speed of the centrifugal bowl, the collected plasma components are circulated in the centrifuge bowl while increasing the speed, and when a predetermined speed is reached, the circuit is switched to extract the platelets. A scheme is disclosed. As a liquid sending pump for circulating plasma,
A roller pump is generally used, and control of the constant speed and acceleration of the pump in the platelet collection step controls the rotation speed of the roller based on the amount of blood pumped per rotation of the roller pump stored in the device. It is done by that.

[0004]

However, when the stored blood transfer amount per rotation of the roller pump is different from the actual blood transfer amount due to factors such as the thickness and material of the pump tube of the platelet collection circuit. There is. In this case, in the above-described platelet collection step, even if the roller pump is rotating at the set pump speed, the actual flow rate may be higher or lower than the set flow rate. If the actual flow rate is higher than the set flow rate, white blood cells or red blood cells may be mixed into the collected platelets. If the actual flow rate is smaller than the set flow rate, the platelet collection rate (collection efficiency) may decrease. It is an object of the present invention to be able to accurately deliver blood plasma at a desired blood supply amount,
It is an object of the present invention to provide a platelet collecting apparatus in which white blood cells and red blood cells are less mixed into collected platelets and have high platelet collection efficiency.

[0005]

Means for achieving the above object are as follows: (1) A rotor having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and the rotation of the rotor A centrifuge for centrifuging the blood introduced from the inflow port in the blood storage space, a first line for connecting a blood collection needle or a blood collection device connection portion and an inflow port of the centrifuge, A second line connected to the outlet of the centrifuge; a third line for anticoagulant injection connected to the first line;
A plasma collection bag having a first tube connected in the middle of the line and a second tube connected to the second line, and a platelet collection circuit comprising a platelet collection bag connected to the second line. A platelet collection device including a blood supply pump provided in a first line, wherein the platelet collection device operates the blood supply pump from the start of blood collection to the time when the centrifuge is filled with anticoagulant-added blood. A platelet collection apparatus comprising: a blood-feeding-pump unit-operating-amount blood-feeding-amount calculating function for calculating a calculated blood-feeding amount per unit operating amount of a blood-feeding pump by using a volume of the centrifuge during rotation. .

(2) In the above-mentioned (1), the platelet collection device may increase the plasma circulation speed of at least the blood supply pump in the platelet collection operation to cause the platelets to flow out of the centrifugal separator so that the platelets are collected by the platelet collection bag. In the platelet collection step of collecting the blood, the blood pump control function for controlling the blood transfer amount of the blood pump using the calculated blood transfer amount calculated by the blood transfer pump unit working amount blood transfer amount calculation function. Preferably, it is provided.

In order to achieve the above object, there are provided (3) a rotor having a blood storage space therein, and an inlet and an outlet communicating with the blood storage space, and the rotation of the rotor causes the rotor to rotate from the inlet. A centrifuge for centrifuging the introduced blood in the blood storage space, a first line for connecting a blood collection needle or a blood collection device connection to an inlet of the centrifuge, A second line connected to the outlet, a third line for anticoagulant injection connected to the first line,
A plasma collection bag having a first tube connected in the middle of the line and a second tube connected to the second line, and a platelet collection circuit comprising a platelet collection bag connected to the second line. A platelet collection device including a blood supply pump provided in a first line, wherein the platelet collection device operates the blood supply pump from the start of blood collection to the time when the centrifuge is filled with anticoagulant-added blood. Using the calculated blood volume calculated from the stored blood transfer volume per unit operating volume and the volume of the centrifuge rotating, the stored blood pump volume per unit operating volume of the blood pump is stored. This is a platelet collection device provided with a blood supply pump unit operation amount blood supply amount correction function for correcting the blood pressure.

(4) In the above-mentioned (3), the platelet collection device may increase the plasma circulation speed of at least the blood supply pump in the platelet collection operation to cause the platelets to flow out of the centrifugal separator, thereby causing the platelets to be collected in the platelet collection bag. In the platelet collection step of collecting the blood, the blood supply pump control function for controlling the blood supply amount of the blood supply pump using the calculated blood supply amount corrected by the blood supply pump unit operation amount blood supply amount correction function. Preferably, it is provided.

(5) In any one of the above (1) to (4), the platelet collection device has a function of storing or calculating the volume of the centrifuge corresponding to the rotation speed of the centrifuge. Is preferred.

(6) In any one of the above (1) to (5), the blood supply pump control function of the platelet collection device uses the calculated blood supply amount after the calculated blood supply amount is calculated. Then, it is preferable to control the blood supply amount of the blood supply pump.

(7) In any one of the above (1) to (6), it is preferable that the platelet collection device includes an operation amount detection unit of a blood feeding pump.

(8) In any one of the above (1) to (7), it is preferable that the blood feeding pump is a roller pump, and the platelet collection device is provided with a rotation amount detecting means of the roller pump. .

(9) In any one of the above (1) to (8), the blood feeding pump is arranged on a centrifugal separator side from a connection portion between the first line and the first tube, Further, the platelet collection device includes a centrifuge drive device for rotating the rotor of the centrifuge, a liquid feed pump for the third line, and opening and closing of a flow path of the platelet collection circuit. A plurality of flow path opening / closing means for performing, a control device for controlling the centrifugal separator driving device, the blood feeding pump, the liquid feeding pump, and the plurality of flow path opening / closing means, further comprising the control device Collecting a blood to which an anticoagulant has been added, separating the collected blood and collecting the separated plasma in the plasma collection bag, and the plasma collection bag collected in the plasma collection step. A plasma circulating step of circulating the plasma in the centrifugal separator through at least one plasma collecting and circulating step; and after the plasma collecting and circulating step, increasing the plasma circulating speed by the blood pump. Performing a platelet collection step of causing platelets to flow out of the centrifuge and collecting the platelets in the platelet collection bag, and returning the blood in the centrifuge after the platelet collection step is completed. It is preferable to control the centrifugal separator driving device, the blood feed pump, the liquid feed pump, and the plurality of flow path opening / closing means so as to perform a platelet collection operation for performing the following.

(10) In the above (9), it is preferable that the control device controls the platelet collection operation to be performed at least twice.

[0015]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A platelet collecting apparatus according to the present invention will be described with reference to an embodiment shown in the drawings. FIG. 1 is a plan view showing a configuration example of a platelet collection circuit used in the platelet collection device of the present invention, FIG. 2 is a plan view of a cassette housing portion of the platelet collection circuit of FIG. 1, and FIG. FIG. 4 is a partially cutaway cross-sectional view showing a state in which a driving device is mounted on a centrifuge used in a platelet collection circuit. FIG. FIG. 5 is a conceptual diagram, and FIG. 5 is a block diagram of a control device used in the platelet collection device of the present invention.

The platelet collection apparatus 1 of the present invention has a rotor 142 having a blood storage space therein, and an inlet 143 and an outlet 144 communicating with the blood storage space.
The centrifuge 20 for centrifuging the blood introduced from the inlet 143 by the rotation of 2 in the blood storage space, the blood collection needle 29 or a blood collection device connection (not shown), and the inlet 143 of the centrifuge 20. A first line 21 for connecting;
A second line 22 connected to the outlet 144 of the centrifuge 20, a third line 23 for injecting anticoagulant connected to the first line 21, and in the middle of the first line 21. Connected first tube 25a and second line 2
A platelet collection circuit 2 for a platelet collection circuit 2 comprising a plasma collection bag 25 having a second tube 25b connected to the second line 22 and a platelet collection bag 26 connected to the second line 22; The blood supply pump 11 provided in the line 21 is provided.

Further, the platelet collection device 1 of the present invention can control the operation amount of the blood feeding pump 11 and the volume of the centrifugal separator 20 during rotation from the start of blood collection to the time when the centrifugal separator 20 is filled with anticoagulant. A blood supply pump unit operation amount blood supply amount calculation function for calculating a calculated blood supply amount per unit operation amount of the blood supply pump 11 by using the function is provided. The platelet collection device 1 of the present invention includes at least the blood pump 1 in the platelet collection operation.
In the platelet collection step of accelerating the plasma circulation speed according to 1 and causing the platelets to flow out of the centrifugal separator 20 and collect the platelets in the platelet collection bag 26, the calculation and transmission calculated by the blood supply pump unit operation amount and the blood supply amount calculation function are performed. It is preferable to provide a blood-feeding pump control function for controlling the blood-feeding amount of the blood-feeding pump 11 using the blood volume.

Further, the platelet collection device of the present invention is not a function for calculating the unit operation amount of the blood supply pump and the blood supply amount, but the function of the blood supply pump 11 from the start of blood collection to the time when the centrifugal separator 20 is filled with the anticoagulant-added blood. Working volume and stored blood pump 11
Using the calculated blood volume calculated from the blood volume per unit operation amount and the volume of the centrifugal separator rotating, the stored blood volume per unit operation amount of the blood pump 11 is corrected. The blood supply pump may be provided with a unit operation amount blood supply amount correction function. In this case, the platelet collection device of the present invention includes a platelet collection step in which at least the blood circulation pump 11 accelerates the plasma circulation speed in the platelet collection operation to cause the platelets to flow out of the centrifuge 20 and collect the platelets into the platelet collection bag 26. In the above, it is preferable to provide a blood feeding pump control function for controlling the blood feeding amount of the blood feeding pump 11 using the calculated blood feeding amount corrected by the blood feeding pump unit working amount blood feeding amount correcting function.

As shown in FIGS. 4 and 5, the platelet collection device 1 includes a centrifuge driving device 10 for rotating the rotor 142 of the centrifuge 20, and a first line 21.
Blood supply pump 11, a liquid supply pump 12 for the third line 23, and a plurality of flow path opening / closing means 81, 82, 83, 84 for opening and closing the flow path of the platelet collection circuit 2.
85, 86, centrifuge drive 10, blood pump 1
1, a control device 13 for controlling the liquid sending pump 12 and the plurality of flow path opening / closing means.

Therefore, the platelet collection circuit 2 will be described first. The platelet collection circuit 2 is a circuit for collecting platelets. The platelet collection circuit 2 includes a blood collection device such as a blood collection needle 29, a connection portion to a blood collection device having a blood collection needle or a blood pool connection portion (blood collection device connection portion), a blood collection needle 29 or a blood collection device connection portion, and a centrifuge. 20 to connect the first line 21 with the first pump tube 21g (blood collection and blood return line) and the outlet 144 of the centrifuge 20 to the first line 21. Of the second line 22 and the first line 21 of the blood supply pump tube 23a.
A third line 23 (an anticoagulant infusion line) having a first tube 25a and a second line 22 connected to a branch connector 21f located closer to the blood collection needle than the pump tube 21g of the first line 21. Plasma collection bag 25 having a connected second tube 25b, a platelet collection bag 26 having a third tube 26a connected to the second line 22, and a buffy coat having a fourth tube 27a connected to the second line 22 A collection bag 27 is provided. The platelet collection circuit 2 may include a connection unit (for example, a metal or synthetic resin needle) for connecting to a blood pool such as a blood bag instead of a blood collection needle.

As the blood collection needle 29, a known metal needle is used. The first line 21 is connected to the blood collection needle side first line 21 a to which the blood collection needle 29 is connected and the inflow port 14 of the centrifuge 20.
3 is connected to the first line 21b on the centrifugal separator side. The first line 21a on the blood collection needle side is formed by connecting a plurality of soft resin tubes. The blood collection needle side first line 21a includes a branch connector 21c for connection to the third line 23, a chamber 21d for removing bubbles and microaggregates, and a second line 22 from the blood collection needle side.
Branch connector 21e for connection with the plasma collection bag 25
Branch connector 21f for connection with the first tube 25a
Is provided. An air-permeable and bacteria-impermeable filter 21i is connected to the chamber 21d. The centrifuge-side first line 21b is connected to a branch connector 21f for connection to the first tube 25a, and has a pump tube 21g formed in the vicinity thereof.

The second line 22 connecting the outlet 144 of the centrifuge 20 and the first line 21 has one end connected to the outlet 144 of the centrifuge 20 and the other end connected to the first line 21. Is connected to the connection branch connector 21e. The second line 22 is connected from the centrifugal separator side to a branch connector 22a for connection with the second tube 25b of the plasma collection bag 25 and the third tube 26a of the platelet collection bag 26, and a tube having a pressure-sensitive filter 22f. Branch connector 22c for connection with the fourth tube 27a of the buffy coat collection bag 27.

One end of the third line 23 is connected to a connection branch connector 21c provided on the first line 21. The third line 23 is connected to the pump tube 23a and the foreign matter removing filter 23 from the connector 21c side.
b, a bubble removing chamber 23c, and an anticoagulant container connecting needle 23d. The plasma collection bag 25 includes a first tube 2 connected to a branch connector 21f located on the blood collection needle side of the pump tube 21g of the first line 21.
5a, a second tube 25b connected to the branch connector 22a of the second line 22. The platelet collection bag 26 includes a third tube 26a connected to the branch connector 22a of the second line 22. The buffy coat collection bag 27 includes a fourth tube 27a connected to the branch connector 22d of the second line 22.

The main part of the platelet collection circuit 2 is as follows.
As shown in FIG. 2, it is of a cassette type. The platelet collection circuit 2 includes all lines (first line, second line, and third line) and all tubes (first tube, second tube, third tube, and fourth tube).
Are partially housed and partially hold them, in other words with a cassette housing 28 in which they are partially fixed. Both ends of the first pump tube 21g and both ends of the liquid feed pump tube 23a are fixed to the cassette housing 28.
g and 23a project from the cassette housing 28 in a loop shape corresponding to the shape of the roller pump. For this reason, the first and liquid feed pump tubes 21g, 23a
Is easy to mount on a roller pump.

Further, the cassette housing 28 has a plurality of openings located in the cassette housing 28. Specifically, the first line 21 on the blood collection needle side portion is exposed from the pump tube 21g, and the first opening portion 91 through which the first flow path opening / closing means 81 of the platelet collection device 1 can enter is provided. The first tube 25a of the collection bag 25 is exposed and the second channel opening / closing means 8 of the platelet collection device 1
Opening 92 through which the plasma collection bag 2 can enter.
5, the third opening 93 through which the third channel opening / closing means 83 of the platelet collection device 1 can enter, and the third tube 26a of the platelet collection bag 26 are exposed and platelet collection is performed. Fourth channel opening / closing means 8 of device 1
The fourth opening 94, the second line 22 through which the fourth
And the fourth tube 27a of the buffy coat collection bag 27
A fifth opening 95 that exposes the second line 22 on the centrifugal separator side (upstream side) from the connection portion with the fifth port 95 and allows the fifth flow path opening / closing means 85 of the platelet collection device 1 to enter; First
Connection with line 21 and buffy coat collection bag 2
7 exposing the second line 22 between the connection portion of the platelet collection device 1 and the second line 22 between the connection portion of the platelet collection device 1 (downstream from the connection portion of the second line 22 and the fourth tube 27a). Sixth opening 96 into which channel opening / closing means 86 can enter.
It has.

On the inner surface of the cassette housing 28, the above-mentioned branch connector is fixed. Further, near the side surface of the cassette housing 28, a line and a tube protruding from the side surface of the housing are held,
Further, a reinforcing tube for preventing bending at the housing portion is provided. Cassette housing 28
Is a box-shaped body in which the portion shown by the broken line in FIG. 2 can be stored. Then, the cassette housing 28
Is formed of a synthetic resin having a certain degree of rigidity.

The platelet collection device 1 includes this cassette housing mounting portion (not shown). For this reason, by mounting the cassette housing 28 to the cassette housing mounting portion of the platelet collection device 1, each line and each tube of the portion exposed from the opening of the cassette housing 28 automatically correspond to the corresponding channel opening / closing means. Be attached. Thus, the circuit can be easily mounted, and platelet collection can be quickly prepared. Further, the platelet collection device 1 is provided with two pumps near the cassette housing mounting portion. For this reason, the cassette housing 28
It is easy to attach the pump tube, which is more exposed, to the pump.

The centrifuge 20 provided in the platelet collection circuit 2 is usually called a centrifugal bowl, and separates blood components by centrifugal force. As shown in FIG. 3, the centrifugal separator 20 has a vertically extending tube 141 having an inlet 143 formed at an upper end thereof, and rotates around the tube 141, and is liquid-tightly sealed to the upper portion 145. Hollow rotor 142
It is composed of A flow path (blood storage space) is formed in the rotor 142 along the bottom and the inner surface of the peripheral wall, and an outlet 144 is formed to communicate with the upper part of the flow path. In this case, the volume of the rotor 142 is, for example,
It is about 100 to 350 ml.

The rotor 142 is rotated under predetermined centrifugal conditions (rotation speed and rotation time) set by the rotor rotation driving device 10 provided in the platelet collection device 1. According to the centrifugation conditions, a blood separation pattern in the rotor 142 (for example, the number of blood components to be separated) can be set. In this embodiment, as shown in FIG.
Blood flows from the inner layer to the plasma layer 13 in the flow path of the rotor 142.
1. The centrifugation conditions are set so that the buffy coat layer 132 and the red blood cell layer 133 are separated.

Next, the platelet collecting apparatus 1 of the present invention shown in FIGS. 4 and 5 will be described. The platelet collection device 1 includes a centrifuge driver 10 for rotating the rotor 142 of the centrifuge 20, a blood pump 11 for the first line 21, and a liquid supply for the third line 23. Pump 12
A plurality of channel opening / closing means 81, 82, 83, 84, 85, 86 for opening and closing the channel of the platelet collection circuit 2;
The apparatus includes a centrifugal separator driving device 10, a blood feeding pump 11, a liquid sending pump 12, and a control device 13 for controlling a plurality of flow path opening / closing means. Further, the platelet collection device 1 includes a turbidity sensor 14 attached to the second line 22 on the centrifugal separator side (upstream side) from the connection portion 22a with the second tube 25b.
Optical sensor 1 mounted above centrifuge 20
5 and a weight sensor 16 for detecting the weight of the plasma collection bag 25.

As shown in FIG. 5, the control unit 13 includes a control unit 50 and a pump controller 5 for the blood pump 11.
3 and pump controller 5 for liquid feed pump 12
4 is provided. The control unit 50 which is a control mechanism of the control device 13
The blood feed pump 11 and the liquid feed pump 12 are electrically connected via pump controllers 53 and 54.
Further, the operation amount detection unit 56 attached to the blood pump 11
Are electrically connected to the control unit 50. As the operation amount detection unit 56, a rotation amount detection unit can be used, and specifically, a rotary encoder can be suitably used. Further, it is also electrically connected to a drive controller 55 provided in the centrifuge drive device (rotor drive device) 10. The control unit 50 also includes data necessary for calculating a calculated blood supply amount in the blood pumping unit operation amount blood supply amount calculation function or the blood supply pump unit operation amount blood supply amount correction function,
A calculation unit for storing the calculation formula and calculating the calculation blood transfer amount is provided.

The platelet collection device 1 of this embodiment stores the amount of blood sent per unit operation amount of the blood feed pump 11,
Hereinafter, this is referred to as a stored blood transfer amount. In addition, the platelet collection device 1 of this embodiment utilizes the working volume of the blood pump 11 and the volume of the centrifuge during rotation from the start of blood collection to the time when the centrifuge 20 is filled with anticoagulant-added blood. Calculate the calculated blood supply amount per unit operation amount of the blood supply pump. And
If there is a difference between the stored blood flow and the calculated blood flow, a correction is made to the stored blood flow as the calculated blood flow, and the calculated blood flow becomes the new stored blood flow, and thereafter, the corrected blood flow is corrected. The blood supply pump is controlled based on the stored blood supply amount. At the start of blood collection, the blood pump is controlled based on the stored blood supply amount stored in advance.

More specifically, the blood collection at the time of the first platelet collection operation is started, and after the blood sensor 202 detects the blood, the total operation amount of the blood pump 11 until the detection of the plasma by the bubble sensor 201 is stored. From the volume of the centrifugal separator rotating at the time of calculation, the calculated blood supply amount per unit operation amount of the blood supply pump 11 is calculated. The operation amount of the blood feeding pump 11 from the time when the blood is detected by the bubble sensor 202 to the time when the blood reaches the centrifugal separator 20, and the amount of blood from the time when the blood flows out of the centrifugal separator 20 until the blood reaches the bubble sensor 201. Blood pump 1
The operation amount of 1 is stored in advance and is excluded from the total operation amount of the blood pump.

Specifically, by dividing the volume of the centrifuge at the time of rotation by the total operation amount (in other words, the total operation unit amount), the calculated blood supply amount per unit operation amount of the blood feeding pump 11 can be reduced. Is calculated. Then, the stored blood flow and the calculated blood flow are compared, and if there is a difference between the stored blood flow and the calculated blood flow, a correction is made so that the stored blood flow is the calculated blood flow. Note that the platelet collection device does not store the blood supply amount per unit operation amount of the blood supply pump 11 and performs the blood collection during the first platelet collection operation under predetermined conditions.
Based on the total amount of operation of the liquid feeding pump until the blood plasma is detected by the centrifugal separator and the stored volume during rotation of the centrifuge, the calculated blood supply amount per unit operation amount of the blood feeding pump 11 is calculated, and the subsequent blood transmission It may be used for controlling a pump.

The blood feeding pump is shown in FIGS.
As shown in (1), in the case of a roller pump, the total operation amount of the pump is detected by the rotation amount detection unit 56. When the liquid sending pump is a peristaltic pump, the total operation amount of the pump is the pump operation time. The detection of the plasma flowing out of the centrifuge 20 is performed by the bubble sensor 20.
The turbidity sensor 14 may be used without using 1.

The platelet collecting apparatus 1 preferably has a function of storing or calculating the volume of the centrifuge corresponding to the rotation speed of the centrifuge. The internal volume of the centrifuge changes because the centrifugal separator receives a larger centrifugal force as the rotation speed increases. That is, the volume of the centrifuge changes according to the rotation speed. In order to accurately calculate the calculated blood supply amount per unit operation amount of the liquid sending pump, it is desirable to calculate using the accurate centrifugal separator volume.
For this reason, the control unit 50 of the plasma separator 1 performs, for example, a rotation speed of the centrifuge at the time of the first platelet collection operation as a storage function of a centrifuge volume corresponding to the rotation speed of the centrifuge. Is a predetermined value, and the volume of the centrifuge at this predetermined value is stored. In addition, the control unit 50 of the plasma separator 1 stores, for example, a correlation equation between the rotation speed and the volume of the centrifuge as a calculation function of the volume of the centrifuge corresponding to the rotation speed of the centrifuge. The volume of the centrifuge at the rotation speed of the centrifuge at the time of the first platelet collection operation may be calculated from the correlation equation.

Then, the control unit 50 determines the unit operation of the liquid feeding pump from the output value from the operation amount detecting unit 56 mounted on the blood feeding pump 11 and the volume of the centrifuge stored or calculated as described above. Calculate the calculated blood transfer volume per volume. Then, the blood supply amount per rotation of the pump roller (in the case of a roller pump) or the blood supply amount per unit time (peristaltic pump) stored in the platelet collection device
However, if the calculated blood supply amount is different from the above, the control unit corrects the stored blood supply amount. It should be noted that the platelet collection device may not store the blood supply amount per unit operation amount. In this case, after the calculated blood supply amount is calculated, the value is stored.

The flow path opening / closing means 81, 82, 83, 8
4, 85 and 86 are all connected to the control device 13, and their opening and closing are controlled by the control device 13. Further, the turbidity sensor 14, the optical sensor 15 mounted above the centrifuge 20, the weight sensor 16 for detecting the weight of the plasma collection bag 25, the bubble sensor 201 and the bubble sensor 202 are also provided by the control device 13 and Signals that are electrically connected and output from them are input to the control device 13. The detection signals from the weight sensor 16, the optical sensor 15, and the turbidity sensor 14 are input to the control device 13 at any time, and the control device 13 includes the turbidity sensor 14, the optical sensor 15, the weight sensor 16, and the bubble sensor. Based on signals from the sensor 201 and the bubble sensor 202, the rotation, stop, and rotation direction (forward / reverse) of each pump are controlled. Control (rotor rotation).

The first channel opening / closing means 81 is provided for opening / closing the first line 21 on the blood collection needle side from the pump tube 21g. Second channel opening / closing means 82
Is provided for opening and closing the first tube 25a of the plasma collection bag 25. The third channel opening / closing means 83
It is provided to open and close the second tube 25b of the plasma collection bag 25. The fourth channel opening / closing means 84 is provided for opening / closing the third tube 26 a of the platelet collection bag 26. The fifth flow path opening / closing means 85 is connected to the second line 22 at a position closer to the centrifugal separator (upstream side) than the connection 22 d between the second line 22 and the fourth tube 27 a of the buffy coat collection bag 27. It is provided for opening and closing.

The sixth flow path opening / closing means 86 is provided between the connecting portion 21e of the first line 21 and the connecting portion of the fourth tube 27a (the connecting portion of the second line 22 and the fourth tube 27a). At a position (downstream side), the second line 22 is provided for opening and closing. The channel opening / closing means includes a line or tube insertion portion, and the insertion portion includes, for example,
It has a clamp that operates with a drive source such as a solenoid, electric motor, cylinder (hydraulic or pneumatic). Specifically, a pneumatic cylinder clamp operated by pneumatic pressure is suitable. The clamp of the flow path opening / closing means operates based on a signal from the control device 13.

As shown in FIG. 3, the rotor driving device 10 holds a rotor rotation driving device housing 151 for accommodating the centrifuge 20, a leg 152, a motor 153 as a driving source, and the centrifuge 20. Disc-shaped fixed base 1
55. The housing 151 includes the leg 1
It is placed and fixed on the upper part of the reference numeral 52. Further, a spacer 15 is provided on the lower surface of the housing 151 by using a bolt 156.
7, a motor 153 is fixed. Motor 15
A fixed base 155 is fitted to the tip of the third rotating shaft 154 so as to rotate coaxially and integrally with the rotating shaft 154, and the bottom of the rotor 142 is fitted to the upper part of the fixed base 155. A recess is formed. In addition, centrifuge 2
0 is fixed to the housing 151 by a fixing member (not shown). Rotor rotation drive 10
Then, when the motor 153 is driven, the fixed base 155 and the rotor 142 fixed to the fixed base
Spin at 000-6000 rpm.

Also, the rotor rotation drive device housing 1
On the inner wall of 51, the interface of the separated blood components in the centrifuge (for example, plasma layer 131 and buffy coat layer 132)
B, buffy coat layer 132 and erythrocyte layer 133
Optical sensor 15 for optically detecting the position of
Are installed and fixed by a mounting member 158. This sensor includes a light source that irradiates light toward the shoulder portion of the centrifuge 20 and a light receiving unit that receives light reflected from the centrifuge bowl and returned. In other words, a light-emitting element such as an LED or a laser and a light-receiving element are arranged in a row, and light reflected by a blood component of light emitted from the light-emitting element is received by the light-receiving element, and the amount of received light is photoelectrically converted. Is configured.

The separated blood components (for example, plasma layer 13
1 and the buffy coat layer 132), the position corresponding to the light receiving element where the amount of received light has changed is detected as the position of the interface B. More specifically,
Based on the difference in the amount of light received by the light receiving portion between the position where the light passes through the centrifuge 20 when the light is filled with a transparent liquid (plasma and water) and when the light is filled with the buffy coat layer, the buffy coat It is detected that the layer has reached the light passage. The position at which the buffy coat layer is detected is adjusted by changing the position at which light passes through the bowl. Usually, once the light passing position is determined, it is fixed there.

The turbidity sensor 14 is for detecting the turbidity of the fluid flowing in the second line 22, and outputs a voltage value corresponding to the turbidity. Specifically, a low voltage value is output when the turbidity is high, and a high voltage value is output when the turbidity is low. The bubble sensor 201 detects a fluid flowing in the first line 21. Specifically, an ultrasonic sensor is used. The bubble sensor 202 is connected to the second line 22.
It detects a fluid flowing in the inside, and specifically, an ultrasonic sensor is used.

The pump tube 21g of the first line 21
Blood pump 11 and third line 23 to which is attached
As the liquid sending pump 12 to which the pump tube 23a is mounted, a non-blood contact type pump such as a roller pump or a peristaltic pump is suitable. In addition, blood pump 1
As 1 (blood pump), a pump capable of sending blood in any direction is used. Specifically, a roller pump capable of normal rotation and reverse rotation is used. The control device is configured to collect the blood to which the anticoagulant has been added, to separate the collected blood and to collect the separated plasma in a plasma collection bag, and to collect the plasma collected by the plasma collection step. A plasma circulation step of circulating the plasma therein to a centrifuge.
Plasma collection and circulation step, and after the plasma collection and circulation step, platelet collection by accelerating the plasma circulation speed by the blood pump to allow platelets to flow out of the centrifuge and collect platelets in the platelet collection bag And a blood return step of returning blood in the centrifuge after the platelet collection step is completed.

The operation of collecting platelets will be specifically described with reference to the flowcharts shown in FIGS. First, the number of platelets to be collected is estimated from the hematocrit value and the platelet concentration of the blood donor that have been measured in advance, and the number of platelet collection operations (cycle number) is set from these values. Then, the third line 23 and the blood collection needle 29 are primed with an anticoagulant, and thereafter, the blood donor is punctured with a puncture needle, and component blood collection is started. An anticoagulant is added to whole blood at a predetermined ratio (1/8 to 1/20, specifically 1/10 with respect to whole blood), and a blood pump (specifically, ,
Roller pump) A predetermined flow rate (250 ml / min or less; preferably 150 to 40
The control device rotates the blood feeding pump so that the flow rate is equal to or less than 60 ml / min (specifically, 60 ml / min or less). The blood collected from the donor is sent to the centrifugal separator 20 via the first line 21 and has a predetermined rotation speed [3000].
８8000 rpm, preferably 4000 to 5800 r
pm], the plasma,
It is separated into buffy coat and red blood cell components. When the plasma flows out of the centrifuge 20, the bubble sensor 201 detects this. The number of rotations of the blood feeding pump from the time when the bubble sensor 202 detects blood to the time when the bubble sensor 201 detects plasma is detected by the rotary encoder 56, and a signal thereof is input to the control unit 50. Then, based on the input signal, the control unit 50
Calculates the total number of rotations of the blood pump 11. At this time, the number of rotations of the blood pump 11 from the time when the blood is detected by the bubble sensor 202 to when the blood reaches the centrifuge 20, and the time until the plasma flows out of the centrifuge 20 and reaches the bubble sensor 201. The rotation speed of the blood pump 11 is stored in advance and is excluded from the total operation amount of the blood pump 11.

The control unit 50 has a function of storing or calculating the volume of the centrifuge 20 at a predetermined rotation speed. The control unit 50 calculates the blood supply amount per rotation of the blood supply pump using the total rotation speed and the volume value of the centrifuge, and calculates the stored blood supply amount per rotation by this calculation transmission. The blood flow is rewritten, and the subsequent flow rate control (in other words, rotation speed control) of the blood feed pump is performed using the calculated blood feed amount. Then, the plasma flowing out of the centrifugal separator 20 is collected in a plasma bag, and a predetermined amount of the plasma (10 to 150 ml,
(Preferably, 20 to 30 ml), the blood feeding is stopped at the time of collection, and the plasma is supplied under predetermined conditions (at a speed higher than the blood collection volume, 60 to 250 ml / min for 10 to 90 seconds, specifically, the first circulation). Is 200ml / min, 30se
At c), a constant-rate plasma circulation is performed, returning to the centrifuge 20 through the first line 21 and the second line 22. The circulating speed in the constant-speed plasma circulation is also controlled using the calculated blood supply amount calculated as described above, so that the plasma can be reliably circulated at the target circulating speed.

Then, an anticoagulant is again added to whole blood (1/8 to 1/20, specifically 1/10 with respect to whole blood).
At a predetermined speed (250 ml / min or less; preferably, 150 to 40 ml / min or less, specifically,
(60 ml / min or less) to the centrifugal separator 20 through the first line 21 to rotate the centrifugal separator 20 at a predetermined rotation speed (3
000-8000 rpm, preferably 4000-58
00 rpm) to separate the blood into plasma, buffy coat, and red blood cell components. When the blood cell interface position inside the centrifuge 20 is detected by the buffy coat interface detection sensor, the blood transmission is stopped. Under predetermined conditions (initial speed 60 ~
80 ml / min, final arrival speed (set speed) 150 to
250 ml / min, acceleration conditions (every second) 2 to 10
ml / min speed increase, circulation time 10-90 sec)
Then, the accelerated plasma circulation is performed by returning to the centrifugal separator 20 through the first line 21 and the second line 22. Since the circulation speed in the accelerated plasma circulation is also controlled using the calculated blood supply amount calculated as described above, the plasma can be reliably circulated at the target circulation speed. Then, under predetermined conditions (blood collection amount: 100 to 2500 / Hct%)
[Ml], specifically, 250 to 1000 / Hct%
[Ml]) A small amount of whole blood is collected while adding an anticoagulant again under the conditions.

After the last blood collection, the plasma is passed through the first and second lines 22 under predetermined conditions.
And stepwisely increase the acceleration under predetermined conditions (stepwise acceleration; 0.1 to 99 ml / min / sec).
c, specifically, 2 to 10 ml / min / sec), platelet collection rate (60 to 300 ml / min; actually 12
0-240 ml / min) and the centrifuge 20
Thus, the outflowing platelets are collected in the platelet collection bag 26. Since the circulation speed in the platelet collection step is also controlled using the calculated blood supply amount calculated as described above, the plasma can be reliably circulated at the target circulation speed. For this reason, there is no reduction in platelet collection efficiency due to the incorporation of white blood cells and red blood cells into the collected platelets due to the target circulation speed exceeding and the target circulation speed not reaching. Specifically, blood collection starts, as shown in FIG.
The blood feeding pump 11 and the liquid feeding pump 12 are operated to collect the blood to which the anticoagulant is added, and the centrifugal separator driving device 10 is operated so that a first predetermined amount of the blood is put into the plasma collection bag 25 from the blood. A first plasma collection step of collecting plasma is performed.

When the first blood collection is started, the blood pump 1
1 starts blood collection at a predetermined speed (for example, 60 ml / min). At this time, the liquid sending pump, which is an anticoagulant pump, is also set at a predetermined speed (for example, 1/10 of the blood pump speed).
Supplies an anticoagulant (eg, ACD solution). The blood collected from the donor is mixed with the ACD solution, flows through the first line 21, passes through the chamber and the first channel opening / closing means 81, and flows into the centrifuge 20. At this time, the sixth passage opening / closing means 86, the fifth passage opening / closing means 85, the second passage opening / closing means 82, and the third passage opening / closing means 83 are closed.
The first flow path opening / closing means 81 and the fourth flow path opening / closing means 84 are open. When the ACD blood is supplied to the centrifugal separator 20, the sterilized air that has entered the centrifugal separator 20 flows through the second line 22, passes through the fourth flow path opening / closing means 84, and enters the platelet collection bag 26. Flows into. Simultaneously with the start of the blood collection process, the centrifuge 20 starts rotating at a predetermined speed, and the centrifuge 20 receives the supply of ACD blood while rotating, so that the blood is centrifuged in the separator, ACD blood (approximately 2) that is separated from the inside into a plasma layer, a buffy coat layer (BC layer) and an erythrocyte layer and exceeds the capacity of the separator.
70 ml), the inside of the centrifuge 20 is completely filled with blood, and the plasma flows out of the outlet of the centrifuge 20. The second connected to the outlet of the centrifuge 20
The bubble sensor 201 attached to the line 22 detects that the fluid flowing in the line has changed from air to plasma, and the control device 13 performs the fourth channel opening / closing based on the detection signal of the bubble sensor 201. The means 84 is closed and the third channel opening / closing means 83 is opened, and the plasma is collected in the plasma collection bag 25. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control device 13. Therefore, when the weight of the plasma collected in the plasma collection bag 25 increases by a first predetermined amount (10 to 150 g, for example, 30 g), the control device 13 closes the first flow path opening / closing means 81 and causes the second Then, the flow path opening / closing means 82 is opened to shift to a constant-speed plasma circulation step.

In the constant-velocity plasma circulation step, the blood collection is temporarily interrupted, and the centrifuge driving device 10 is operated to remove the plasma collected in the plasma collection bag 25 to the centrifugal separator 20.
At a constant speed. When entering the constant-speed plasma circulation step, the control device 13 maintains the closed state of the first flow path opening / closing means 81 and the open state of the second flow path opening / closing means 82,
The ACD pump 12 stops, and the blood pump 11 operates at a predetermined speed (60 to 250 ml / min, for example, 200 ml / m).
In), the plasma in the plasma collection bag 25 is sent to the centrifugal separator 20 rotating at a predetermined speed (initial rotor rotational speed calculation value) through the second flow path opening / closing means 82. At the same time, the plasma flowing out of the centrifuge 20
4. The plasma collection bag 2 through the third channel opening / closing means 83
Flow into 5. When a predetermined time (10 to 90 seconds, for example, 30 seconds) has elapsed since the start of the constant-speed plasma circulation step, the control device 13 closes the second flow path opening / closing means 82 and switches the first flow path opening / closing means 81 to Open to move to the second plasma collection step. The first plasma circulation is at least 60 ml / m
It is preferable to perform the process at a flow rate of at least in for 10 seconds or more.

In the second plasma collection step, the blood supply pump 11 and the liquid supply pump 12 are operated to collect the blood to which the anticoagulant has been added, and the optical sensor is usually operated by increasing the amount of plasma in the bag. When 15 detects the buffy coat layer of the separator, this signal is sent to the control device 13, and the control device 13 closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82. Then, the process proceeds to the accelerated plasma circulation step.

Specifically, the blood feeding pump 11 starts collecting blood at a predetermined speed (for example, 60 ml / min). At this time, the liquid supply pump, which is an anticoagulant pump, simultaneously supplies the anticoagulant (for example, ACD-A liquid) at a predetermined speed (for example, 1/10 of the blood pump speed). Blood collected from the donor is mixed with the ACD solution, flows into the centrifugal separator 20 rotating at a predetermined speed (calculated value of the initial rotor speed), and plasma is collected in the plasma collection bag 25. Normally, when the optical sensor 15 detects the buffy coat layer of the separator due to an increase in the amount of plasma in the bag, this signal is sent to the control device 13 and the control device 13 Is closed, the second flow path opening / closing means 82 is opened, and the process proceeds to the accelerated plasma circulation step. In the second plasma collection step, plasma is collected until the sensor 15 detects a buffy coat (BC interface: interface between the plasma layer and the buffy coat layer).

In the accelerated plasma circulation step, the blood collection is temporarily interrupted, and the centrifuge driving device 10 is operated to circulate the plasma in the plasma collection bag 25 to the centrifuge 20 while accelerating. At this time, the blood pump speed is lower than the constant-speed plasma circulation step, for example, 60 ml / mi.
n and the final speed is 150-200 ml / mi
Accelerate until n is reached. As acceleration conditions, a speed of 2 to 10 ml / min increases every second, 200 m
It is performed with an arrival time of 1 / min about 14 to 70 seconds. After the end of this circulation step, the process proceeds to FIG. 7, and a small amount plasma collection step for adjusting the interface is performed.

As shown in FIG. 7, in the step of collecting a small amount of plasma for adjusting the interface, in order to keep the position of the buffy coat layer constant in the subsequent platelet collecting step regardless of the donor,
Blood is collected for a predetermined supply amount of red blood cells. The supply amount of red blood cells is defined as a value obtained by dividing the blood collection amount by the hematocrit value of the donor, and the blood collection amount is generally about 12 ml. Also in this blood collection, the blood feeding pump 11 operates at a predetermined speed (for example, 60
(ml / min). At this time, the liquid supply pump, which is an anticoagulant pump, is also simultaneously driven at a predetermined speed (for example, 1/10 of the blood pump speed) with an anticoagulant (eg, ACD
-A solution). Blood collected from the donor was AC
It is mixed with the solution D and flows into the centrifugal separator 20 which rotates at a predetermined speed (calculated value of the initial rotor speed) to collect a small amount of plasma. The control device 13 calculates the collection time from the set collection amount and the pump speed, and when the collection time has elapsed,
End blood collection. Then, the control device 13 closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to a platelet collection step.

After the above steps are completed, a platelet collecting step is performed in which the plasma circulation speed by the blood feed pump 11 is accelerated to cause the platelets to flow out of the centrifuge 20 and collect the platelets in the platelet collection bag 26. The platelet collection step is also called a so-called acceleration process. In this step, the blood pump speed is, in this example, 120 ml /
The controller 13 operates the blood pump so as to accelerate from 2 min to 240 ml / min by 2 to 20 ml / min every predetermined time (for example, 1 second), and when the speed reaches a predetermined speed (for example, 200 ml / min). And maintain that speed until the platelet collection process is complete.

When the platelet collection step starts, the turbidity sensor 14 detects the turbidity of the liquid passing therethrough, and the turbidity is output as a voltage value by the sensor, and the output signal is input to the control device 13. When the speed of the blood pump increases and reaches approximately 120 to 240 ml / min, platelets contained in the buffy coat layer remaining in the centrifuge 20 flow out. Turbulence sensor 14 when platelets flow out
When the turbidity of the liquid passing through the portion increases and the voltage value output from the sensor decreases by 0.2 V, the third channel opening / closing means 83 closes and the fourth channel opening / closing means 84 opens, and The platelet-rich plasma flowing out of the separator 20 is collected in the platelet collection bag 26. The voltage value output from the turbidity sensor 14 is converted into a platelet concentration by the control device 13, and the platelet concentration in the platelet collection bag 26 during platelet collection is calculated. The platelet concentration in the platelet collection bag 26 once reaches the maximum concentration and then decreases. At the point in time when it is detected that the maximum concentration has been reached, the platelet collection step ends, and the flow returns to the blood return step.

Next, a blood return step of returning the blood in the centrifuge 20 is performed. The control device 13 controls the blood pump 11
Is rotated in the reverse direction, the first channel opening / closing means 81 is opened, and the red blood cell layer remaining in the centrifuge 20 is returned to the donor from the first line 21. Thus, the first (first) platelet collection operation is completed. Such a platelet collection operation is repeatedly performed for a preset number of cycles. In the platelet collection operation except for the last round, the buffy coat is drained from the centrifuge bowl 20 before the blood return step, and the buffy coat collection step of collecting the buffy coat in the buffy coat collection bag 27 is performed. It is preferable to perform a buffy coat return step of returning to the centrifuge bowl 20 before the plasma collection step.

[0059]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention comprises a stored blood whose specific gravity has been measured in advance, a platelet collection circuit in which the inner diameter of a pump tube has been measured in advance with a circuit configuration as shown in FIG. A platelet collection device to be prepared was prepared. In this platelet collection device, the roller pump 11 is rotated so as to reach the set flow rate based on the blood supply amount per rotation of the roller pump stored in the control device 13. The blood sent by the roller pump is sent to the centrifugal separator 20 via the first line 21, and the blood plasma, buffy coat,
It is separated into each component of red blood cells.

At this time, the rotation speed of the roller pump 11 from the detection of blood by the bubble sensor 202 to the detection of plasma flowing out of the centrifuge 20 by the bubble sensor 201 is detected by the rotary encoder 56. Are input to the control unit 50. And
The control unit 50 calculates the total number of rotations of the roller pump 11 based on the input signal. Note that the number of rotations of the roller pump 11 from when blood is detected by the bubble sensor 202 to when the blood reaches the centrifuge 20 and the roller pump from when blood plasma flows out of the centrifuge 20 to reach the bubble sensor 201. The number of rotations 11 is stored in advance and is excluded from the total number of rotations of the blood feeding pump 11. The control unit 50 stores the volume of the centrifuge 20 at the set rotation speed, and stores the total rotation speed and the centrifuge 20
Is calculated using the volume value of the roller pump, the stored blood transfer amount per rotation is rewritten to this calculated blood transfer amount, and the flow rate control of the blood transfer pump ( In other words, the rotation speed control) is performed using the calculated blood supply amount.

Then, the platelet collection circuit is set in the platelet collection device, and the stored blood is set at a set flow rate of the roller pump 11 of 60 mL / min. Sent inside. Thus, after obtaining the calculated blood supply amount, the stored blood is transferred using the same pump tube to a volume of 620 m.
The roller pump 11 was rotated and sent for a time corresponding to L, and collected in a blood bag. Then, the weight of the preserved blood collected in the blood bag was measured using an electronic balance, and converted into the blood specific gravity measured in advance to obtain a blood transfer amount (actual blood flow rate) (Examples 1 to 3). Further, as described above, the calculation of the blood supply amount per one rotation of the roller pump 11 is not performed, and therefore, the stored blood supply amount per one rotation is equivalent to the stored blood supply amount corresponding to 620 mL. Only the roller pump 11 was rotated to send the blood to the blood bag, and the amount of collected blood (actual blood flow) was measured in the same manner (Comparative Examples 1 to 3).

(Experimental Results) Examples 1-3 and Comparative Example 1
The actual blood flow rates in Tables 1 to 3 were as shown in Table 1.

[0063]

[Table 1]

[0064]

The platelet collection apparatus of the present invention utilizes the operation amount of the blood pump from the start of blood collection to the time when the centrifuge is filled with anticoagulant-added blood and the volume of the centrifuge during rotation. The blood pump has a function of calculating the unit operation amount of the blood pump and the function of calculating the calculated blood supply amount per unit operation amount of the blood pump. Therefore, in the platelet collection device of the present invention, in the platelet collection, the blood supply amount of the blood supply pump is controlled based on the accurate blood supply amount per unit operation amount of the blood supply pump. Platelets in a good state can be collected at a high efficiency without a decrease in platelet collection efficiency due to mixing of white blood cells and red blood cells into the collected platelets and failure to reach the set blood supply amount.

In addition, the platelet collection device of the present invention provides the operation amount of the blood pump from the start of blood collection to the time when the centrifuge is filled with the anticoagulant-added blood and the stored operation amount of the blood pump per unit operation amount. Using the calculated blood volume calculated from the blood volume and the volume of the centrifuge when rotating, the stored blood volume per unit volume of the blood pump is corrected to correct the stored blood volume per unit volume. It has a blood volume correction function. Therefore, in the platelet collection device of the present invention, in the platelet collection, the blood supply amount of the blood supply pump is controlled based on the accurate blood supply amount per unit operation amount of the blood supply pump. Platelets in a good state can be collected at a high efficiency without a decrease in platelet collection efficiency due to mixing of white blood cells and red blood cells into the collected platelets and failure to reach the set blood supply amount.

[Brief description of the drawings]

FIG. 1 is a plan view showing a configuration example of a platelet collection circuit used in a platelet collection device of the present invention.

FIG. 2 is a plan view of a cassette housing part of the platelet collection circuit of FIG. 1;

FIG. 3 is a partially cutaway sectional view showing a state in which a driving device is mounted on a centrifuge used in a platelet collection circuit.

FIG. 4 is a conceptual diagram of one embodiment of the platelet collection device of the present invention with a platelet collection circuit mounted.

FIG. 5 is a block diagram of a control device used in the platelet collection device of the present invention.

FIG. 6 is a flowchart for explaining the operation of the platelet collection device of the present invention.

FIG. 7 is a flowchart for explaining the operation of the platelet collection device of the present invention.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 platelet collecting device 2 platelet collecting circuit 10 centrifuge driving device 11 blood feeding pump 12 liquid feeding pump 13 controller 14 turbidity sensor 15 optical sensor 16 weight sensor 20 centrifuge 21 first line 22 second line 23 Third line 25 Plasma collection bag 26 Platelet collection bag 29 Blood collection needle

Claims (8)

[Claims] A rotor having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and centrifuging blood introduced from the inlet by rotation of the rotor in the blood storage space. A first line for connecting a centrifuge to be separated, a blood collection needle or a blood collection device connection portion to an inlet of the centrifuge, and a second line connected to the outlet of the centrifuge A third line connected to the first line for injecting an anticoagulant; a second tube connected to the first line and a second line connected in the middle of the first line; A platelet collection circuit including a plasma collection bag having a tube and a platelet collection bag connected to the second line, and a platelet collection device including a blood supply pump provided in the first line; Collection The operation of the blood pump is calculated using the operation amount of the blood pump from the start of blood collection to the time when the centrifugal separator is filled with anticoagulant and the volume of the centrifuge during rotation. A platelet collection device, comprising: a blood supply pump unit operation amount for calculating a blood volume; and a blood supply amount calculation function. 2. The platelet collection device according to claim 1, wherein the platelet collection device includes: a platelet collection step of accelerating at least a plasma circulation speed by a blood pump in a platelet collection operation, causing platelets to flow out of the centrifuge, and collecting platelets in the platelet collection bag. A blood supply pump control function for controlling the blood supply amount of the blood supply pump by using the calculated blood supply amount calculated by the blood supply pump unit operation amount blood supply amount calculation function. Item 3. The platelet collection device according to Item 1. 3. A rotor having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and blood introduced from the inlet by rotation of the rotor is centrifuged in the blood storage space. A first line for connecting a centrifuge to be separated, a blood collection needle or a blood collection device connection portion to an inlet of the centrifuge, and a second line connected to the outlet of the centrifuge A third line connected to the first line for injecting an anticoagulant; a second tube connected to the first line and a second line connected in the middle of the first line; A platelet collection circuit including a plasma collection bag having a tube and a platelet collection bag connected to the second line, and a platelet collection device including a blood supply pump provided in the first line; Collection The calculated blood volume calculated from the amount of blood pump operation from the start of blood collection to the time the centrifuge is filled with anticoagulant-added blood and the stored blood volume per unit operation volume and the centrifuge A platelet having a blood-feed-pump unit-operating-amount blood-feeding-amount correcting function for correcting the stored blood sending amount per unit operating amount of the blood-feeding pump using the volume at the time of rotation. Sampling equipment. 4. The platelet collection device according to claim 1, wherein the platelet collection device includes: a platelet collection step of accelerating at least a plasma circulation speed of a blood pump in a platelet collection operation, causing platelets to flow out of the centrifuge, and collecting platelets in the platelet collection bag. A blood supply pump control function for controlling the blood supply amount of the blood supply pump using the calculated blood supply amount corrected by the blood supply pump unit operation amount blood supply amount correction function. Item 4. A platelet collection device according to item 3. 5. The platelet collection device according to claim 1, wherein the platelet collection device has a function of storing or calculating a volume of the centrifuge corresponding to the rotation speed of the centrifuge. . 6. The blood supply pump control function of the platelet collection device controls the blood supply amount of the blood supply pump using the calculated blood supply amount after the calculated blood supply amount is calculated. The platelet collection device according to any one of claims 1 to 5, wherein the platelet collection device is a platelet collection device. 7. The platelet collection device according to claim 1, wherein the platelet collection device includes an operation amount detection unit for a blood pump. 8. The blood supply pump is disposed closer to a centrifuge than a connection portion between the first line and the first tube, and the platelet collection device is provided with the blood pump of the centrifuge. A centrifugal separator driving device for rotating the rotor, a liquid feed pump for the third line, a plurality of flow path opening / closing means for opening / closing a flow path of the platelet collection circuit, A control device for controlling a blood vessel driving device, the blood feeding pump, the liquid feeding pump and the plurality of flow path opening / closing means, further comprising: collecting and collecting blood to which an anticoagulant is added. Separating the separated blood and collecting the separated plasma into the plasma collection bag, and plasma circulating the plasma in the plasma collection bag collected by the plasma collection step to the centrifuge And at least one plasma collection / circulation step consisting of a step and, after completion of the plasma collection / circulation step, accelerating the plasma circulation speed by the blood feeding pump to cause the platelets to flow out of the centrifugal separator and to remove the platelets. The platelet collection step is performed so as to perform a platelet collection step of collecting the platelet in the platelet collection bag, and after the platelet collection step, performing a blood return step of returning blood in the centrifuge. The platelet collection device according to any one of claims 1 to 7, wherein the platelet collection device controls a separator driving device, the blood feeding pump, the liquid feeding pump, and the plurality of flow path opening / closing means.