JP2001231502A - Method for producing cruciferous plant and its processed product - Google Patents

Abstract

PROBLEM TO BE SOLVED: To produce a cruciferous plant containing GABA which has hypotension activities in high concentration, and its dried powder, and further to provide a squeezed juice of the cruciferous plant and a dried powder of the squeezed juice. SOLUTION: A cruciferous plant is subjected to anaerobic treatment and/or temperature-keeping treatment at 25-50 deg.C (GABA-enriching treatment), and the treated product is dried to provide the cruciferous plant or its dried powder. When treating the cruciferous plant, a microwave treatment is carried out to provide the cruciferous plant keeping the green color and maintaining the GABA content, its dried powder, the squeezed juice of the cruciferous plant and the drier powder thereof.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a cruciferous plant rich in γ-aminobutyric acid (hereinafter sometimes referred to as GABA). More specifically, a cruciferous plant that retains green color and has a high content of GABA, its juice,
The present invention relates to those dry powders, their production methods, their foods, beverages, and pharmaceutical raw materials.

[0002]

2. Description of the Related Art Since GABA has a blood pressure lowering effect, foods containing a large amount of GABA have been studied for people with hypertension. For example, conventionally, in the process of producing tea, plucked tea leaves are placed under anaerobic conditions, and GAB is added to the tea leaves.
A so-called GABARON tea in which A is accumulated in a large amount is known. JP-A-8-173111 describes that coffee leaf tea having a high GABA concentration was obtained by treating fresh coffee leaves in an anoxic condition, followed by heat treatment and drying at a high temperature of 110 ° C. or higher. . Further, Japanese Patent Application Laid-Open
No. 2,059,893 discloses a method of irradiating tea leaves with infrared rays.
It is described to improve the content of BA.

[0003] Tea leaves obtained by these methods are extracted by pouring boiling water, and useful ingredients that can be ingested are limited to water-soluble ones.

[0004] On the other hand, cruciferous plants such as kale contain many components such as vitamins, and are attracting attention as health foods. Brassicaceae plants such as kale are squeezed or made into a dry powder for drinking and edible use. Also, cruciferous plants, dry powder,
In order to maintain the green color of the squeezed juice and the dried powder of the squeezed juice, a so-called blanching treatment is generally performed in which the cruciferous plant is treated at a high temperature such as hot water. However, in the hot water treatment, there is a high possibility that the active ingredient disappears, and it is impossible to obtain a cruciferous plant which maintains the active ingredient and retains a green color.

For example, cruciferous plants (for example, cabbage) are shredded, added with vitamin C, heat-treated,
A method of squeezing into juice is disclosed in Japanese Patent Application Laid-Open No. 10-4284.
No. 1 publication. However, this method has a problem that heat-sensitive nutrients are decomposed.

Further, Japanese Patent No. 2796227 describes a method of obtaining dry powder of cruciferous plants by combining preliminary drying for 12 to 13 hours and far infrared rays and treating at a relatively low temperature. . However, the conventional method has a drawback that only the nutrients of cruciferous plants such as kale are available for food. Therefore,
If a nutrient or a component having a certain medicinal effect (for example, GABA) is further added to the cruciferous plant, a large amount of the active component (for example, GAB) is directly produced instead of the above-mentioned extraction.
Since A) can be taken, the effect as a further health food is expected.

[0007]

Accordingly, there is a need for a cruciferous plant dry powder, juice, and juice dry powder to which new nutrients or medicinal ingredients have been added.

[0008]

Means for Solving the Problems As a result of earnest studies on cruciferous plants, the present inventors have found that cruciferous plants contain GABA having a hypotensive action,
The inventors succeeded in increasing the GABA content in cruciferous plants, and completed the present invention. The cruciferous plant was particularly suitable for the treatment for increasing the GABA content, and a cruciferous plant with an increased GABA content could be obtained.

According to the present invention, there is provided a cruciferous plant having an increased GABA content, and the cruciferous plant having an increased GABA content is directly used for food. Also,
By drying the cruciferous plant having the enhanced GABA content, a dry cruciferous plant powder rich in GABA is provided. Furthermore, by squeezing a cruciferous plant with an increased GABA content, a juice rich in GABA is provided, and a dried juice powder rich in GABA is provided. Accordingly, there is provided a health food which has a new added value of having a blood pressure lowering effect in addition to conventional cruciferous plant juice and dried powder.

According to the present invention, GABA is used in an amount of 50 mg / 100 g.
It relates to a cruciferous plant containing the above.

[0011] In a preferred embodiment, the cruciferous plant retains a green color.

According to the present invention, a branching process is performed, and
10 mg / 100 g of γ-aminobutyric acid that retains green color
The present invention relates to a cruciferous plant or a squeezed juice thereof.

[0013] The present invention also provides a method for producing a cruciferous plant containing GABA of 50 mg / 100 g or more, comprising a step of performing anaerobic treatment and / or a heat treatment at 25 to 50 ° C on the cruciferous plant. About.

In a preferred embodiment, the method further includes a step of performing microwave processing.

[0015] In a preferred embodiment, the treated cruciferous plant retains a green color.

Further, the present invention provides a method for preparing GABA at 50 mg / 1.
The present invention relates to the extraction of cruciferous plants containing at least 00 g.

In a preferred embodiment, the juice of the cruciferous plant has a green color.

[0018] The present invention further comprises a step of subjecting the cruciferous plant to anaerobic treatment and / or a heat treatment at 25 to 50 ° C, and a step of pressing the treated cruciferous plant to obtain 50 mg / kg GABA. The present invention relates to a method for producing cruciferous plant juice containing 100 g or more.

Further, the present invention provides a method for squeezing a cruciferous plant, which comprises the step of performing anaerobic treatment and / or heat treatment at 25 to 50 ° C., containing GABA at 50 mg / 100 g.
The present invention relates to a method for producing cruciferous plant juice containing g or more.

In a preferred embodiment, the method for producing a squeezed juice further includes a step of performing a microwave treatment.

In a preferred embodiment, the juice obtained retains a green color.

According to the present invention, GABA is added at 180 mg /
The present invention relates to a cruciferous plant dry powder containing 100 g or more.

Further, the present invention provides a method for producing GABA, comprising the steps of performing anaerobic treatment and / or heat treatment at 25 to 50 ° C. on a cruciferous plant and drying.
The present invention relates to a method for producing a dry powder of cruciferous plants containing at least 80 mg / 100 g.

Furthermore, the present invention relates to a method for preparing GABA at 10 mg /
The present invention relates to a dry powder of a cruciferous plant which retains a green color and contains 100 g or more.

In a preferred embodiment, the composition further contains 0.1 mg / 100 g or more of vitamin B1 and 100 mg / 100 g or more of vitamin C.

The present invention further comprises a step of subjecting the cruciferous plant to microwave treatment, and a step of drying the treated cruciferous plant.
The present invention relates to a method for producing a green cruciferous plant dry powder containing at least mg / 100 g.

In a preferred embodiment, the method further includes a step of performing an anaerobic treatment and / or a heat treatment at 25 to 50 ° C.

Further, the present invention provides a step of subjecting a cruciferous plant to anaerobic treatment and / or a heat treatment at 25 to 50 ° C., a step of blanching the treated cruciferous plant, and a step of blanching. 10 mg / 1 γ-aminobutyric acid, including a step of drying a cruciferous plant
The present invention relates to a method for producing a green cruciferous plant dry powder containing at least 00 g.

In the present invention, GABA is added at 180 mg /
The present invention relates to a dry powder of a cruciferous plant juice containing 100 g or more.

Further, the present invention provides a step of subjecting the cruciferous plant to anaerobic treatment and / or a heat treatment at 25 to 50 ° C., a step of pressing the treated cruciferous plant, and a step of compressing the obtained cruciferous plant. GAB including a step of drying the juice
The present invention relates to a method for producing a dry powder of squeezed cruciferous plant containing 180 mg / 100 g or more of A.

In the present invention, GABA is added at 10 mg / 1.
The present invention relates to a dried powder of squeezed green brassicaceae plant containing at least 00 g.

[0032] In a preferred embodiment, the dried powder of the juice of a green cruciferous plant further comprises:
Vitamin B1 0.1mg / 100g or more, Vitamin C
Of 100 mg / 100 g or more.

The present invention comprises a step of subjecting a cruciferous plant to a microwave treatment, a step of pressing the treated cruciferous plant, and a step of drying the obtained juice. Containing
The present invention relates to a method for producing a dried powder of squeezed green brassicaceae plant.

[0034] The present invention also provides a step of subjecting the juice of cruciferous plants to a microwave treatment, and a step of drying the obtained juice to obtain 10 mg / l GABA.
The present invention relates to a method for producing a dry powder of squeezed juice of a cruciferous plant which retains green color and contains at least 00 g.

The present invention also relates to a step of performing anaerobic treatment and / or a heat treatment at 25 to 50 ° C. on a cruciferous plant, a step of blanching the treated cruciferous plant, A method for producing a dried powder of squeezed green cruciferous plant containing γ-aminobutyric acid of 10 mg / 100 g or more, comprising a step of squeezing the obtained cruciferous plant and a step of drying the obtained juice. About.

Further, the present invention provides a step of performing anaerobic treatment and / or a heat-retaining treatment at 25 to 50 ° C. on the juice of cruciferous plants, and a step of blanching the treated juice of cruciferous plants. , And drying.
The present invention relates to a method for producing a dry powder of squeezed green cruciferous plants, which contains 10 mg / 100 g or more of γ-aminobutyric acid.

The present invention also relates to a method for increasing the GABA content of a cruciferous plant, comprising a step of anaerobically treating the cruciferous plant and / or keeping it warm at 25 to 50 ° C.

The present invention further relates to a method for increasing the GABA content of cruciferous plant juice, which comprises a step of anaerobic treatment and / or a heat treatment at 25 to 50 ° C. of the cruciferous plant juice.

The present invention also provides a process for squeezing a cruciferous plant GABA, comprising the steps of anaerobic treatment of a cruciferous plant and / or heat treatment at 25 to 50 ° C. and pressing the treated cruciferous plant. It relates to a method for increasing the content.

The present invention further relates to a method for preserving the green color of a cruciferous plant, its juice or a dry powder thereof, comprising a step of microwave-treating the cruciferous plant or its juice.

Further, the present invention relates to a cruciferous plant having an enhanced GABA content, a juice of a cruciferous plant having an increased GABA content, a dry powder of a cruciferous plant having an enhanced GABA content, a green-brown crucifer Food, beverage, or pharmaceutical ingredient containing either dry powder of a family of grasses, dried powder of juice of a cruciferous plant with an enhanced GABA content, or dried powder of juice of a cruciferous plant that retains green color About.

In a preferred embodiment, the cruciferous plant is kale, the cruciferous juice is kale juice, the cruciferous dry powder is kale dry powder, and the cruciferous plant is dried. The dried powder of squeezed juice is the dried powder of squeezed kale juice.

[0043]

BEST MODE FOR CARRYING OUT THE INVENTION The plants of the Brassicaceae family used in the present invention include cabbage, broccoli, cauliflower, kale, radish, wasabi, komatsuna and the like.
Of these, kale is particularly preferred. In addition, when referring to a cruciferous plant, it includes the case where it refers to a part of leaves, stems, flowers and the like. Further, the term "brassaceous plant" includes a fragmented cruciferous plant.

The fragmentation of cruciferous plants is performed by a method commonly used by those skilled in the art, such as slicing or chopping, to obtain a fragmented plant. The flakes include those in the form of a slurry, but the slurries are made with a juicer,
The processing is performed by a blender or the like.

The cruciferous plant containing GABA in a specific amount or more of the present invention has been completed by subjecting the cruciferous plant to a treatment for increasing the GABA content (hereinafter referred to as a GABA enrichment treatment). Hereinafter, the GABA enrichment process will be described first.

(GABA-enriched treatment) G of cruciferous plants
The ABA enrichment treatment is performed on a cruciferous plant, preferably a cut, shredded or fragmented cruciferous plant.
The anaerobic treatment, the heat retention treatment at 25 to 50 ° C., or both of them are performed simultaneously.

Anaerobic treatment means treatment with a gas containing almost no oxygen or no oxygen. Including vacuum conditions. As the gas, carbon dioxide gas and nitrogen gas are preferably used.

The method of the heat retaining treatment is not limited. Warm water treatment, infrared irradiation treatment, warm air treatment, incubator treatment and the like can be mentioned. Infrared irradiation treatment is preferably used.

The time of the anaerobic treatment and / or the heat retention treatment is preferably 30 minutes to 24 hours, more preferably 1 to 6 hours. The temperature of the anaerobic treatment and / or the heat retention treatment is about 2
The temperature is preferably 5 to 50C, more preferably about 30 to 45C, and most preferably about 40C. Below 25 ° C,
If it exceeds 50 ° C., the content of GABA is difficult to increase.

GABA treatment also includes storing and storing cruciferous plants in a constant temperature room at a constant temperature (for example, 25 to 50 ° C.).

The anaerobic treatment and / or the heat retention treatment are preferably carried out under such conditions that the cruciferous plant is not dried, but may be carried out under such a condition that the plant is dried.

The above anaerobic treatment and / or heat retention treatment (G
GABA of cruciferous plants obtained by ABA enrichment treatment
The content is increased. For example, as shown in Table 1 of Example 1 of the present invention, the GABA content is increased 3.6 times in broccoli, about 6 times in cabbage, and about 6.2 times in kale.

(Brassic Plant Containing GABA of 50 mg / 100 g or More)
Cruciferous plants containing 100 g or more are obtained by GABA enrichment treatment.

In general, the GABA content of cruciferous plants is
Since kale is the highest and is about 30 mg / 100 g,
A cruciferous plant containing GABA at 50 mg / 100 g or more has never existed before and is novel. A GABA-enriched cruciferous plant of the present invention,
In particular, the content of GABA in kale is preferably 80 mg / 100 g or more, more preferably 100 mg / 100 g or more, further preferably 200 mg / 100 g or more, and furthermore 250 mg / 100 g or more.

As regards the content of GABA, when referring to cruciferous plants and squeezed juice, it refers to the weight based on the wet weight, and when referring to the cruciferous plant dry powder and the squeezed dry powder, the weight based on the dry weight is used. Say.

(Squeezing of Brassicaceae Plants Containing at least 50 mg / 100 g of GABA)
The cruciferous plant juice containing at least mg / 100 g,
For cruciferous plants, preferably cut, chopped or shredded cruciferous plants, GABA
It is obtained by performing an enrichment treatment and then squeezing the GABA-enriched cruciferous plant, and applying a method commonly used by those skilled in the art, such as filtration and centrifugation, if necessary. The term “squeezed juice” in the present specification includes an extract, and includes green juice obtained by pressing, ethanol extract from cruciferous plants, and warm to hot water extract of cruciferous plants.

Further, the GABA of the present invention is used in an amount of 50 mg / 10
Squeezing of cruciferous plants containing 0 g or more is performed by first squeezing a cruciferous plant, preferably a cut, shredded or fragmented cruciferous plant, and if necessary, a person skilled in the art such as filtration and centrifugation. Can be obtained by applying a commonly used method, and also by performing a GABA enrichment treatment on the juice in the same manner as described above.

The above anaerobic treatment and / or heat retention treatment (G
GABA of cruciferous plant juice by ABA enrichment treatment
The content is increased. In general, the GABA content of cruciferous plants is highest in kale, which is about 30 mg / 100 g. Therefore, the extraction of cruciferous plants containing GABA of 50 mg / 100 g or more has never existed before. It is new. The GABA content of the GABA-enriched cruciferous plants of the present invention, especially kale juice, is 8%.
0 mg / 100 g or more is preferable, and 100 mg / 100
g or more, more preferably 200 mg / 100 g or more, and furthermore 250 mg / 100 g or more.

A cruciferous plant that is blanched and contains 10 mg / 100 g or more of γ-aminobutyric acid that retains green color can be obtained by blanching the above GABA-enriched cruciferous plant.
The cruciferous plant juice that is blanched and contains 10 mg / 100 g or more of γ-aminobutyric acid that retains green color is obtained by squeezing the blanched plant after GABA-enrichment treatment or after GABA-enrichment treatment. Obtained by blanching squeezed juice. Most of GABA disappears by the blanching treatment (5 mg / 10
0 g or less), by performing a GABA enrichment treatment and performing a branching treatment to obtain 10 mg / 10
GABA of 0 g or more remains.

(Dry Powder of Brassicaceae Plants Containing at least 180 mg / 100 g of GABA)
The dry powder of cruciferous plants containing 0 mg / 100 g or more is obtained by drying the above-mentioned GABA-enriched cruciferous plants. Usually, GAB of cruciferous plants
The A content is the highest in kale, around 30 mg / 100 g, and when dried, the GABA content is about 170
mg / 100 g. Therefore, GABA obtained by drying the GABA-enriched cruciferous plant,
Cruciferous plant dry powders containing at least mg / 100 g have never been present and are novel.
In addition, a dry powder having a required GABA content can be obtained by appropriately selecting the GABA enrichment treatment method and conditions. For example, 300 mg / 100 g or more, 40
0 mg / 100 g or more, 500 mg / 100 g or more, 1
A dry powder having a GABA content of 000 mg / 100 g or more and 1500 mg / 100 g or more can be obtained. As an upper limit, about 4000 mg / 100 g of dry powder can be obtained.

The drying of cruciferous plants is carried out when the water content is 10%.
Below, it is preferably performed so as to be 5% or less. As the drying method, a method that does not raise the temperature so much, such as hot air drying, far-infrared drying, electromagnetic wave drying, and freeze drying, is preferable from the viewpoint of preserving nutrients. Drying is preferably performed at a temperature as low as possible (for example, 50 ° C. or lower). Even when heating and drying,
For example, the heat treatment may be performed at 50 to 80 ° C, preferably 55 to 65 ° C.

When the cruciferous plant is dried, an alkaline aqueous solution may be attached to the cruciferous plant before the treatment in order to prevent the green color fading.

The dried product is further pulverized to obtain a dry powder. For pulverization, a method commonly used by those skilled in the art is employed. For example, it is pulverized by a crusher, a mill, a blender, a stone mill, or the like. If necessary, the dried and ground crucible can be sieved, and those passing through 30 to 250 mesh may be used as cruciferous dry powder. If the particle size is smaller than that passing through 250 mesh, it is difficult to handle during further processing of the cruciferous plant dry powder,
When the particle size is larger than that passing through 30 mesh, uniform mixing of the cruciferous plant dry powder and other food materials may be hindered.

(Dry Powder of Cruciferous Plant Juice Containing GABA of 180 mg / 100 g or More) GABA of the Present Invention
The dried powder of cruciferous plant juice containing at least 180 mg / 100 g of the cruciferous plant should be dried after GABA-enriched cruciferous plant juice or cruciferous plant juice is subjected to GABA enrichment treatment. Is obtained by Usually, the GABA content of cruciferous plants is the highest in kale, about 30 mg / 100 g, and when this juice is dried, the GABA content is about 170 mg / 100 g. Therefore, a dried powder of a cruciferous plant juice containing 180 mg / 100 g or more of GABA obtained by drying the juice from the GABA-enriched cruciferous plant or the GABA-enriched juice has hitherto been used. And was new.

In the case of cruciferous plants, especially kale, G
By appropriately selecting the ABA enrichment method and conditions,
The amount of GABA contained in the dried powder of the juice is 180 mg
０００4000 mg / 100 g. Preferably 50
0 mg / 100 g or more, more preferably 800 mg / 1
The amount is at least 00 g, more preferably at least 1000 mg / 100 g, most preferably at least 1500 mg / 100 g. In a preferred case, the dried powder of kale juice contains GABA more than 6 times that of conventional dried powder of juice.

As a method for drying the juice, spray drying or freeze drying is preferable. Upon drying, from the viewpoint of maintaining a green color, the pH of the juice may be adjusted in advance to a range of 6 to 9 by means such as adding an alkaline aqueous solution to the juice, and then dried. During drying, if necessary, excipients such as dextrin, cyclodextrin, starch, and maltose may be added.

The above-mentioned cruciferous plant, cruciferous juice, dry powder of cruciferous plant, and dry powder of cruciferous plant juice can maintain a green color by using an alkali or the like. , Tend to change the taste. Usually, when producing dry powder of cruciferous plants, etc., in order to deactivate enzymes involved in alteration (fading of green color, change of flavor) and the like, generally at high temperatures such as hot water treatment and steaming treatment. That is, a so-called branching process is performed. However, it was found that GABA disappeared in the blanching process.

Therefore, the present inventors have studied means to replace the conventional branching processing in which GABA is hardly reduced.
It has been found that the object can be achieved by microwave treatment. Further, after performing the GABA enrichment treatment, by performing a blanching treatment, it was found that a desired amount of GABA remained, and a green cruciferous plant,
It has been found that the squeezed juice or the dried powder described below can be obtained.

(Microwave treatment) The microwave irradiation treatment is mainly intended to deactivate an enzyme that discolors the green appearance of cruciferous plants. On the other hand, excessive microwave irradiation treatment may cause the loss of nutrients and active ingredients such as GABA and vitamins contained in the plant of the family Abnaceae. Therefore, it is desirable that the irradiation of the microwave be performed within a range in which the enzyme involved in the fading is inactivated, the green color can be maintained, and the effectiveness is not lost due to the excessive irradiation. Such a range may be appropriately set based on the output of the irradiation device, the wavelength of the microwave, the irradiation time, the type and the amount of cruciferous plants.

For example, when a microwave of 2450 MHz and 500 W is applied, 0.5 to 10 minutes, preferably 0.5 to 5 minutes, more preferably 0.5 to 5 minutes per 100 g (wet weight) of kale, a cruciferous plant. Process for 5 to 1 minute. If it is less than 0.5 minutes, the enzyme is not sufficiently deactivated, and the color is easily discolored. When the treatment is performed for 10 minutes or more, there is a possibility that the active ingredient is reduced. However, even if reduced, as shown in the examples below, the GA
The BA content is high and the green color is retained.

The blanching treatment includes high-temperature treatments such as the above-mentioned hot water treatment and steaming treatment generally performed by those skilled in the art. When blanching is performed by adding an appropriate amount of sodium bicarbonate or sodium chloride alone or in combination during the treatment, the green color is kept vivid. Of course, there is no problem if the branching processing and the microwave processing are combined.

(Cruciferous Plant Containing GABA of 10 mg / 100 g or More and Retaining Green)
A green cruciferous plant containing mg / 100 g or more can be obtained by microwave-treating a cruciferous plant. Alternatively, it is obtained by performing a microwave treatment after the GABA enrichment treatment. In the blanching treatment described below performed to maintain the green color of the cruciferous plant, the amount of GABA contained in the cruciferous plant is about 2 mg / 100 g. 100
g, preferably 20 mg / 100 g or more, is obtained. Further, by combining the GABA enrichment treatment and the microwave treatment, a green cruciferous plant containing GABA of 50 mg / 100 g or more can be obtained.

Conventionally, as described above, a green cruciferous plant contains only about 30 mg / 100 g of GABA. Therefore, a green cruciferous plant containing GABA of 50 mg / 100 g or more is new. .

By appropriately selecting the GABA enrichment treatment method and conditions, a cruciferous plant containing a necessary amount of GABA can be obtained. For example, 70 mg / 1
00g or more, 100mg / 100g or more, 150mg /
A cruciferous plant having a GABA content of 100 g or more and 200 mg / 100 g or more can be obtained. With respect to the upper limit, a cruciferous plant having a GABA content of up to about 500 mg / 100 g can be obtained.

The above GABA enrichment treatment and microwave treatment are relatively gentle treatments, and therefore, the vitamins contained in the cruciferous plant, particularly vitamin B, are contained.
1. Vitamin C is maintained without loss even after drying. Preferably, vitamin B1 is 0.1 mg / 100
g or more, Vitamin C can be included in cruciferous plants holding green color at 100 mg / 100 g or more.

Furthermore, a green cruciferous plant containing GABA of 50 mg / 100 g or more can be obtained by subjecting a cruciferous plant to GABA enrichment treatment and then to blanching treatment.

(Squeezing of Brassicaceae Plants Containing at least 50 mg / 100 g of GABA)
Of a green cruciferous plant containing 50 mg / 100 g or more can be obtained by performing a combination of a GABA-enriching treatment and a microwave treatment on a cruciferous plant and pressing the treated cruciferous plant. The microwave processing may be performed before the GABA enrichment processing or may be performed after the GABA enrichment processing.

[0078] In addition, juice of a cruciferous plant retaining a green color containing GABA of 50 mg / 100 g or more can also be obtained by combining the juice of a cruciferous plant with a GABA enrichment treatment and a microwave treatment.

Conventionally, as described above, a green cruciferous plant contains only about 30 mg / 100 g of GABA. Therefore, the extraction of a green cruciferous plant containing GABA of 50 mg / 100 g or more is not possible. It is new.

By appropriately selecting the GABA enrichment treatment method and conditions, it is possible to obtain a cruciferous plant juice containing a necessary amount of GABA. For example, 70m
g / 100 g or more, 100 mg / 100 g or more, 200
mg / 100g or more, 300mg / 100g or more GA
It is possible to obtain a juice of a cruciferous plant having a BA content.
With respect to the upper limit, juice of cruciferous plants having a GABA content of up to about 600 mg / 100 g can be obtained.

As described above, the juice of a green cruciferous plant of the present invention preferably contains vitamin B
1 is 0.1mg / 100g or more, vitamin C is 100m
g / 100 g or more.

(Dry Powder of Brassicaceae Plants Containing GABA of 10 mg / 100 g or More Having Green Color) GA
A green cruciferous plant dry powder containing 10 mg / 100 g or more of BA is obtained by subjecting cruciferous plants to microwave treatment. As shown in the examples described below, GABA is 2 mg / 100 g in green dry powder obtained by treating cruciferous plants with hot water.
Only degree is included. On the other hand, the cruciferous plant dry powder that retains the green color obtained by performing the microwave treatment contains GABA in an amount of 10 mg / 100 g or more. Therefore, a dry powder of a green cruciferous plant containing GABA of 10 mg / 100 g or more is novel.

When the cruciferous plant is kale, GABA
Can be 20 mg / 100 g, 100 mg / 100 g
100 g or more, and 150 mg / 100 g
Or more. Combined with GABA enrichment treatment,
200 mg / 100 g or more, further 500 mg / 10
It can be 0 g or more.

As described above, the dried powder of the cruciferous plant which retained green color contained 0.1 mg / vitamin B1.
It may contain 100 g or more, and vitamin C of 100 mg / 100 g or more.

Further, a dry powder of a cruciferous plant which retains green color and contains GABA of 10 mg / 100 g or more can be obtained by subjecting a cruciferous plant to GABA enrichment treatment and then to blanching treatment.

(Dry Powder of Juice of Brassicaceae Plant Containing GABA of 10 mg / 100 g or More) The dry powder of green juice of cruciferous plant containing GABA of 10 mg / 100 g or more is: , Of a cruciferous plant or its juice by microwave treatment. As described above, the green dry powder obtained by performing a conventional blanching treatment such as a hydrothermal treatment on a cruciferous plant contains only about 2 mg / 100 g of GABA. Can not be. Therefore, a dry powder of a green cruciferous plant juice containing GABA of 10 mg / 100 g or more is novel.

When the cruciferous plant is kale, the content of GABA in the dried powder of the juice can be 20 mg / 100 g, 100 mg / 100 g or more, and
It may be 0 mg / 100 g or more. When combined with GABA enrichment treatment, it can be 200 mg / 100 g or more, and even 500 mg / 100 g or more.

As described above, vitamin B1 is contained in the dried powder of the juice of the cruciferous plant having a green color in an amount of 0.1%.
mg / 100g or more, vitamin C is 100mg / 100
g or more.

Further, the dried powder of the cruciferous plant squeezed green containing at least 10 mg / 100 g of GABA is subjected to a GABA enrichment treatment, followed by a blanching treatment. Can also be obtained by drying.

(Utilization of Brassicaceae Plants High in GABA Content) Brassicaceae plants high in GABA content, their dried powder, squeezed juice, and dried powder of squeezed juice obtained in the present invention can be prepared by a person skilled in the art, if necessary. Commonly used methods, for example,
Sterilization may be performed by a method such as airflow sterilization, high-pressure sterilization, or heat treatment. Also, if necessary, excipients, fillers, binders, thickeners, emulsifiers, coloring agents, flavors, food additives, mixed with seasonings, etc., depending on the application, such as powder, granules, tablets, etc. It can be formed into a form, and can be further added to various foods and drinks for use in eating and drinking.

Further, the GA produced by the method of the present invention
A cruciferous plant containing a large amount of BA, its dry powder,
Pure juice of GABA can be purified using juice or juice powder as a raw material. In particular, kale is useful as these raw materials.

[0092]

EXAMPLES (Example 1) Infrared treatment was performed using fresh leaves of kale, fresh leaves of cabbage, and broccoli shredded to a size of about 5 mm. Infrared treatment is performed using a 400 W infrared radiation device, each object is sealed so that moisture does not evaporate, a temperature sensor is inserted, and while controlling the internal temperature to 40 ° C., Irradiated for 1 hour.

After the infrared treatment, GABA (GABA) was measured using an automatic amino acid analyzer under the following conditions. <Operation conditions of automatic amino acid analyzer> Model: JLC-500 / V (JEOL Ltd.) Column: LCR-6, 4 mm x 90 mm (JEOL Ltd.) Mobile phase: lithium citrate buffer (JEOL Ltd.) ) P-21 (pH 2.98, Li 0.105 mol / l) 0 → 16.3 min P-12 (pH 3.28, Li 0.26 mol / l) 16.3 → 36.1 min P-13 (pH 3.46, Li 0.80 mol / l) 36.1 → 56.0 min P-14 (pH 2.83, Li 1.54 mol / l) 56.0 → 63.4 min P-15 (pH 3.65, Li 1.54 mol / l) 63.4 → 80.0 min Reaction solution: Ninhydrin / hydridantine TS (Wako Pure Chemical Industries, Ltd.) ) Temperature: Column 35 ° C (0 → 16.3 min), 64 ° C (15.3 → 31.0 min) 44 ° C (31.0 → 44.4 min), 72 ° C (63.4 → 80.0 min) Reaction tank 135 ° C Flow rate: 0.50 ml of mobile phase / min Reaction solution 0.30 ml / min Measurement wavelength: 570 nm

Table 1 shows the results.

[0095]

[Table 1]

The results in Table 1 indicate that the heat treatment increased the GABA content in cruciferous plants. GABA content increased about 3.6-fold for broccoli, about 5.9-fold for cabbage, and about 6.2-fold for kale.

Example 2 Fresh kale leaves were treated with infrared rays, warm air and warm water. The infrared ray treatment was performed in the same manner as in Example 1. The hot air treatment was performed by blowing hot air at 40 ° C. in an incubator under sealed conditions, and the warm water treatment was performed while keeping the temperature in 40 ° C. hot water. After performing each treatment, GABA was measured. Table 2 shows the results.

[0098]

[Table 2]

The results in Table 2 show that regardless of the treatment method, the GABA content in the kale leaves is increased by the heat treatment. In particular, the infrared treatment increased about 6.7 times, and the hot air treatment and the hot water treatment each increased 3.2 times.
GABA content increased by a factor of 3.8 and 3.8.

(Example 3) Fresh kale leaves, chopped fresh kale leaves having a size of 5 mm, and squeezed juice obtained by crushing and filtering the fresh leaves with a mixer at 40 ° C for 1 hour in the same manner as in Example 1 After that, the GABA content was measured. Table 3 shows the results.

[0101]

[Table 3]

The results in Table 3 indicate that the GABA content was increased regardless of the type of the treatment target, that is, by enriching the fresh leaves, shredded fresh leaves, and fresh leaf juices with GABA. Is shown. GABA was increased by a factor of 6 to 7 or more compared to before the treatment.

(Example 4) Since it was considered that the infrared treatment was effective, the optimum temperature of the infrared treatment was examined. After treating the kale leaves at the temperatures shown in Table 4, GABA was measured. Table 4 shows the results.

[0104]

[Table 4]

The results in Table 4 show that the optimum temperature is around 40 ° C. Below 25 ° C. and above 55 ° C., the GABA content tends to decrease.

(Example 5) Since it was considered that the infrared treatment at 40 ° C was effective, the optimal time of the infrared treatment was examined. After the kale leaves were treated at 40 ° C. for the times shown in Table 5, GABA was measured. Table 5 shows the results.

[0107]

[Table 5]

The results shown in Table 5 show that G is approximately 10 minutes after the start of processing.
It can be seen that the ABA content tends to increase,
In the case of the treatment for 30 minutes or more, it increases about three times, and about 1 to 6
It shows that the maximum value is reached in time.

(Example 6) Next, using fresh kale leaves,
The increase in GABA content by anaerobic treatment was examined.
The anaerobic treatment is as follows: kale leaves are packed in a plastic bag, evacuated and filled with nitrogen gas.
The temperature was kept at 5 ° C., and the test was carried out for the time shown in Table 6. Note that 40
The treatment at ° C. was performed by irradiating infrared rays under anaerobic conditions.
Table 6 shows the results.

[0110]

[Table 6]

The results in Table 6 show that the anaerobic treatment at 25 ° C.
It was found that the GABA content in the kale leaves increased by a factor of three or more, indicating that the GABA content also increased in the anaerobic treatment. Further, it can be seen that the GABA content is increased by about 7.4 times by performing the heat retention treatment (infrared treatment) at 40 ° C.

Example 7 Fresh kale leaves were kept at 40 ° C. for 1 hour (infrared ray treatment), dried with a dryer at 60 ° C. so that the water content became 5% or less, and further crushed with a blender. Thus, a GABA-enriched kale dry powder was obtained. Raw kale leaves not subjected to the heat treatment were similarly treated to obtain a kale dry powder not subjected to the GABA enrichment treatment. Table 7 shows the results of measuring the GABA content of each dry powder.

[0113]

[Table 7]

The results in Table 7 show that the GABA content of the GABA-enriched dry powder was increased about 8.7-fold.

(Example 8) After keeping the raw kale leaves at 40 ° C for 1 hour (infrared treatment), they were crushed and squeezed with a mixer and filtered to remove GABA-enriched kale juice. Juice was obtained. Kale fresh leaves that were not subjected to the heat treatment were similarly treated to obtain kale juice that had not been GABA-enriched. Table 8 shows the results of measuring the GABA content of each juice.

[0116]

[Table 8]

The results in Table 8 show that the GABA enrichment treatment
This indicates that the GABA content in the juice has increased 6.5-fold.

(Example 9) Kale fresh leaves were kept at 40 ° C for 1 hour (infrared treatment), crushed and squeezed with a mixer, and filtered to remove the fiber content. After obtaining the juice, it was freeze-dried and further crushed by a blender to obtain a GABA-enriched kale juice dried powder.
The same process is applied to fresh kale leaves that are not heat-treated.
A dried kale juice powder that was not ABA-enriched was obtained.
Table 9 shows the results of measuring the GABA content of each dried juice powder.

[0119]

[Table 9]

The results in Table 9 show that the GABA-enriched squeezed dry powder has about a 9.6-fold increase in GABA content.

Example 10 Young kale leaves were picked, washed with water, drained, and cut into pieces of about 3 cm square. 100 g of the powder was output from a 2450 MHz, 500 W output device (microwave oven, RE-121 manufactured by Sharp) as shown in Table 1.
The microwave irradiation treatment was performed for the time described in 0.
Then, using a drier (manufactured by Sanyo, MOV-112S), the product was dried at 60 ° C. for 6 hours and pulverized with a blender to obtain kale powder. The water content was less than 5%.

(Comparative Example 1) 100 g of shredded kale
5 ° C, put into 1L hot water, hold for 3 minutes, immediately 5 ° C
And immersed for about 5 minutes to cool. After cooling, the mixture was dehydrated by a centrifuge for about 45 seconds, dried and powdered as in Example 10. However, drying required 10 hours.

(Comparative Example 2) 100 g of shredded kale was
The solution was poured into 1 L of hot water at 95 ° C. containing 7.5 g of sodium chloride and 7.5 g of sodium bicarbonate, dried and powdered as in Comparative Example 1.

Table 10 shows the results. In Table 10, the term "untreated" means that the chopped kale before the treatment was dried as it is or at 60 ° C for 6 hours, pulverized by a blender, and powdered. The figures are dry weight equivalents,
The unit is mg / 100 g. Vitamins were measured by high performance liquid chromatography.

[0125]

[Table 10]

From the results shown in Table 10, the microbe-treated cruciferous plant (kale) retained GABA as compared with the conventional cruciferous plant blanched with hot water or saline added with sodium bicarbonate. further,
Vitamin B1 and vitamin C were also sufficiently retained.

Further, the kale subjected to microwave treatment and the kale obtained by the conventional treatment were each stored in an aluminum package in an environment of 40 ° C. and 75% humidity and shielded from light for 2 months. The green color of the microwave-treated kale did not fade and retained a vivid green color, but the fresh leaves of the untreated kale faded from green and changed to a slightly brownish color.

(Example 11) Kale leaves were picked and
Infrared treatment was performed at 0 ° C. for 1 hour. The infrared treatment was performed using an infrared irradiation device, sealing was performed so that moisture did not evaporate, and control was performed while controlling the internal temperature to 40 ° C. Then, a blanching process was performed with hot water,
The powder was dried in the same manner as described above to obtain a powder. On the other hand, fresh kale leaves cut into pieces of 3 cm square were similarly subjected to infrared treatment to perform GABA enrichment treatment, then blanched with hot water, pressed to obtain juice, and dried to obtain powder. The GABA content of the kale leaf, the juice, the dried powder of the kale leaf, and the dried powder of the juice after the GABA enrichment treatment and the blanching treatment were measured.

As a comparison, without performing the GABA enrichment treatment,
After blanching with hot water, it was dried and powdered.
Table 11 shows the results.

[0130]

[Table 11]

This result indicates that the GABA enrichment treatment-branching treatment leaves GABA, which has been almost completely lost, and that 10 mg / 100 g or more of GABA remains even in plants. . That is, in the comparative example, since the GABA-enrichment treatment was not performed, kale leaves were dried at 1.5 mg / 100 g, and dried kale leaves were dried.
Although only 2 mg / 100 g of GABA remained, in the example, 10.1 mg / 100 g of kale leaves,
14.1 mg / 100 g of GABA remained in the squeezed juice, 67 mg / 100 g of dried kale leaf powder, and 85 mg / 100 g of the squeezed powder remained.

Example 12 Fresh kale leaves were picked and subjected to infrared treatment in the same manner as in Example 11 to enrich GABA. Thereafter, microwave irradiation treatment and drying treatment were performed in the same manner as in Example 10. For comparison, GABA
Without performing the enrichment treatment, the blanching treatment was performed with hot water, and the dried and powdered kale was measured for GABA content. Table 12 shows the results. In addition, the leaf column is obtained by performing only the microwave treatment, and the numerical value is a value with respect to the wet weight. The column of dry powder is a value obtained by drying after microwave treatment, and is a value based on a dry weight.

[0133]

[Table 12]

The results in Table 12 show that G was obtained by the method of the present invention.
ABA is contained at an extremely high concentration of 1200 mg / 100 g. In other words, it indicates that a dry powder of a cruciferous plant which retains green color and contains 240 times or more GABA can be obtained as compared with the conventional method.

As described above, in the conventional method, although the green color is maintained, GABA, vitamin B1 and vitamin C, which are the active ingredients in the kale, almost disappear, but the microwave-treated kale has GABA, vitamin B
It can be seen that 1 and vitamin C are maintained almost as it is, and the usefulness of the cruciferous plant of the present invention is understood.

[0136]

Industrial Applicability According to the present invention, a cruciferous plant having an increased GABA content is provided, and the cruciferous plant having an increased GABA content is directly used for food. Further, by drying the cruciferous plant having the enhanced GABA content, a dry cruciferous plant powder rich in GABA is provided. Furthermore, by extracting a cruciferous plant having an increased GABA content, GABA is obtained.
Is provided, and a dried juice powder is further provided, which is rich in GABA. Accordingly, there is provided a health food which has a new added value of having a blood pressure lowering effect in addition to conventional cruciferous plant juice and dried powder.

Further, in producing a cruciferous plant, a dry powder of a cruciferous plant, a juice of a cruciferous plant, and a dry powder of a juice of a cruciferous plant, the GABA content is reduced by performing microwave treatment. Instead, a dry powder of a cruciferous plant, a cruciferous plant dry powder, a cruciferous plant juice, and a cruciferous plant juice that retains a green color can be obtained.

The obtained Brassicaceae plant having an increased GABA content or a reduced green color without decreasing the GABA content, its dried powder, squeezed juice, or squeezed dried powder can be used as it is for eating and drinking. It can be mixed with excipients, extenders, binders, thickeners, emulsifiers, colorants, flavors, food additives, seasonings, etc., and molded into powders, granules, tablets, etc. depending on the application. It can be further added to various foods and drinks and provided for eating or drinking, or used as a raw material for pharmaceuticals.

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Claims (17)

[Claims] 1. A method for producing a cruciferous plant which retains green color and contains 10 mg / 100 g or more of γ-aminobutyric acid.
Performing an aminobutyric acid enrichment treatment; and (b) performing at least one of a blanching treatment and a microwave treatment on the treated cruciferous plant,
Method. 2. A method for producing a dry powder of cruciferous plants, which retains green color and contains 10 mg / 100 g or more of γ-aminobutyric acid, wherein (a) γ-aminobutyric acid is added to a cruciferous plant. (B) a step of performing at least one of a blanching treatment and a microwave treatment on the treated cruciferous plant; and (c) a cruciferous plant treated with the (b) treatment. A method comprising the step of drying. 3. A method for producing a cruciferous plant juice which retains green color and contains 10 mg / 100 g or more of γ-aminobutyric acid. (B) pressing the treated cruciferous plant; and (c) performing microwave treatment on the pressed juice obtained by pressing. 4. A method for producing a dried powder of a cruciferous plant juice, which retains a green color and contains 10 mg / 100 g or more of γ-aminobutyric acid. performing a γ-aminobutyric acid enrichment treatment;
(B) pressing the treated cruciferous plant;
(C) performing a microwave treatment on the squeezed juice obtained by pressing; and (d) drying the squeezed juice subjected to the (c) treatment. 5. A method for producing cruciferous plant juice, which retains green color and contains 10 mg / 100 g or more of γ-aminobutyric acid. performing a γ-aminobutyric acid enrichment treatment; and (b) performing a microwave treatment on the treated juice. 6. A method for producing a dry powder of a cruciferous plant juice, which retains a green color and contains 10 mg / 100 g or more of γ-aminobutyric acid. On the other hand, the method includes a step of performing γ-aminobutyric acid enrichment treatment; (b) a step of performing microwave treatment on the treated juice; and (c) a step of drying the treated juice of (b). ,Method. 7. A method for producing a cruciferous plant which retains green color and contains 10 mg / 100 g or more of γ-aminobutyric acid, comprising a step of performing a microwave treatment on the cruciferous plant. Manufacturing method. 8. A method for producing a cruciferous plant juice, which retains green color and contains 10 mg / 100 g or more of γ-aminobutyric acid, wherein a microwave treatment is performed on the cruciferous plant juice. A method for producing a cruciferous plant juice, comprising: 9. At least one of a blanching process and a microwave process is performed, and a green color is maintained.
A cruciferous plant other than kale or a juice thereof containing at least 10 mg / 100 g of γ-aminobutyric acid. 10. A dry powder of cruciferous plants other than kale, which has been subjected to at least one of a blanching treatment and a microwave treatment, retains a green color and contains at least 10 mg / 100 g of γ-aminobutyric acid. 11. Furthermore, vitamin B1 is added in an amount of 0.1 mg / day.
The dry powder of cruciferous plants other than kale according to claim 10, which contains 100 g or more and vitamin C of 100 mg / 100 g or more. 12. Drying of juice of cruciferous plants other than kale, which has been subjected to at least one of a blanching treatment and a microwave treatment, retains a green color, and contains 10 mg / 100 g or more of γ-aminobutyric acid. Powder. 13. Further, vitamin B1 is added in an amount of 0.1 mg / day.
13. The dried powder of squeezed cruciferous plants other than kale according to claim 12, comprising at least 100 g and at least 100 mg / 100 g of vitamin C. 14. An anaerobic treatment of at least one plant selected from the group consisting of kale, cabbage, and broccoli or their squeezing and / or 25-
A method for increasing the γ-aminobutyric acid content of the plants or their squeezed products, comprising a step of keeping the temperature at 50 ° C. 15. An anaerobic treatment of at least one plant selected from the group consisting of kale, cabbage, and broccoli or their squeezed and / or 25-
A method for producing kale, cabbage, broccoli, or their squeezed juice with an increased content of γ-aminobutyric acid, comprising a step of keeping the temperature at 50 ° C. 16. A method for producing a dried powder of kale, cabbage, broccoli or a juice thereof having an increased γ-aminobutyric acid content, wherein (a) the method comprises the steps of: performing a γ-aminobutyric acid enrichment treatment; and (b) the treated kale;
Drying the cabbage, broccoli or their juice. 17. A cruciferous plant other than the kale according to claim 9, or a juice thereof, a dry powder of a cruciferous plant other than the kale according to claim 10 or 11, or the kale according to claim 12 or 13. A food, beverage, or pharmaceutical ingredient containing any of the dried powders of cruciferous plant juices other than.