JP2002000228A - Antihypertensive food containing material derived from barley young leaf - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a food material having high antihypertensive effect and comprising a material derived from barley young leaves and another material containing ingredient(s) having antihypertensive activity other than GABA(γ- aminobutyric acid). SOLUTION: This food material comprises a material derived from barley young leaves and another material containing ingredient(s) having antihypertensive activity other than GABA.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to γ-aminobutyric acid,
The present invention relates to a novel food material containing a material derived from wheat young leaves containing vitamins, minerals, dietary fiber and the like in high concentration.

[0002]

BACKGROUND OF THE INVENTION Wheat young leaves are rich in vitamins, minerals and dietary fiber, have antihypertensive effects, adsorb harmful substances, improve intestinal environment, suppress cholesterol absorption, prevent a sharp rise in postprandial blood glucose, superoxide dismutase. (SO
D) has attracted attention as a health food having effects such as activating D). At present, when wheat young leaves are used as a food material, they are mainly used as wheat young leaves powder (Japanese Patent No. 2544302) or as wheat young leaves juice powder.

[0003] Food components having an antihypertensive effect include γ-aminobutyric acid (γ-aminobutyric acid).
c acid, hereinafter referred to as GABA). GABA
Is a kind of amino acid produced in vivo by the decarboxylation of glutamic acid. GABA is present in the brain and spinal cord of mammals and is known to act as a neurotransmitter of the inhibitory system. GABA is also present in plants, and germ rice, green tea and the like contain relatively large amounts of GABA. GA
BA improves cerebral blood flow, increases oxygen supply, and enhances cerebral metabolism.
It is used to treat head trauma, sequelae of the cerebral artery, headache, tinnitus, and decreased motivation.

[0004] As a food that pays attention to GABA, for example, so-called GABARON tea is known, in which tea leaves that have been extracted are placed under anaerobic conditions in the process of producing tea and GABA is accumulated in large amounts in the tea leaves. JP-A-8-173111 discloses that
It is described that coffee leaf tea having a high GABA concentration was obtained by a method of treating fresh coffee leaves in an anoxic condition, followed by heat treatment at a high temperature of 110 ° C. or higher and drying treatment. Further, Japanese Patent Application Laid-Open No. 9-205989 discloses that the tea leaves are irradiated with infrared rays to improve the content of GABA.

[0005]

A material derived from wheat young leaves,
Provided is a food material having an excellent antihypertensive effect in combination with a material containing an ingredient having an antihypertensive action other than GABA.

[0006]

Means for Solving the Problems The present inventors have conducted intensive studies on the antihypertensive effect of a food using a material derived from wheat young leaves, and found that natural wheat young leaves contain GABA, It was prepared so that the antihypertensive effect was synergistically enhanced by the combination of the raw material and a raw material containing an ingredient having an antihypertensive action other than GABA, and GABA in wheat young leaves was retained or enriched. The present inventors have found that the use of a material derived from wheat young leaves further enhances the antihypertensive effect, and completed the present invention.

[0007] The present invention relates to a material derived from wheat young leaves and GABA.
And a material containing an ingredient having an antihypertensive action other than the above.

[0008] Preferably, the material derived from wheat young leaves is treated so that GABA naturally present therein is increased (hereinafter referred to as GABA enrichment treatment).

Preferably, the component having an antihypertensive effect other than GABA is an angiotensin converting enzyme inhibitory component.

[0010] Preferably, the material containing a component having an antihypertensive action other than GABA is a hydrolyzate of milk protein or fish protein or a fermented product of lactic acid bacteria.

Preferably, the material containing a component having an antihypertensive action other than the above GABA is Tochu leaf or an extract thereof.

Preferably, the component having an antihypertensive effect other than GABA is a peptide, a glycoside, or geniposidic acid.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, the material derived from wheat young leaves refers to the powder of wheat young leaves, the fragment of wheat young leaves and its dry powder, the juice of wheat young leaves and its dry powder, the extract of wheat young leaves and its dry powder. And so on.

As a raw material of the raw material derived from wheat young leaves of the present invention, for example, barley, wheat, rye, oat, Italian ryegrass and other young barley leaves (may contain stems) are used. These wheat young leaves are processed as quickly as possible because the green color of the wheat young leaves fades over time after harvesting. Preferably, young leaves harvested at the beginning of tillering or at the beginning of heading (height is about 20 to 40 cm) are optimally used, and more preferably, young leaves of barley are used.

After harvesting, the wheat leaves are washed with water or the like, drained and treated as they are, or cut to an appropriate length (for example, 10 cm) and used.

The wheat young leaves may be sliced. The shredding can be performed by a method commonly used by those skilled in the art, such as slicing or shredding, to shred a plant. Shredding also includes slurrying. The slurrying is performed by a mixer, a juicer, a blender, a mass colloider or the like, and the young wheat leaves become a mushy porridge (a suspension of a liquid and a solid).
Wheat young leaf juice obtained by squeezing after shredding can also be used. Squeezing may be performed by centrifuging and / or filtering the flaked wheat young leaves.

In a preferred embodiment of the present invention, the wheat leaf and the wheat leaf processed as described above are treated so as to increase the GABA contained therein.

The method of GABA enrichment treatment of wheat young leaves includes:
For example, a method including an anaerobic treatment or a heat retention treatment may be mentioned. The GABA enrichment treatment may be performed before the washing.

As used herein, the term “anaerobic treatment”
It means to be kept in anaerobic condition, and it means to treat with almost oxygen-free or oxygen-free gas. Including vacuum conditions. As the gas, carbon dioxide gas and nitrogen gas are preferably used.

There is no limitation on the method of heat retention. Warm water treatment, infrared irradiation treatment, incubator treatment and the like can be mentioned.

The time of the anaerobic treatment and / or the heat retention treatment can be usually 10 minutes to 24 hours. Preferably,
It is performed for 1 to 24 hours, more preferably for 1 to 12 hours. The temperature of the anaerobic treatment or the heat treatment is about 20 to 50
C is preferred, about 30-45 C is more preferred, and 40 C
Before and after is most preferred. If the temperature is lower than 20 ° C. or higher than 50 ° C., the content of GABA is difficult to increase.

The wheat young leaves thus obtained have a higher GABA content than untreated wheat young leaves,
It is increased by a factor of at least two, preferably at least three, more preferably at least five.

When wheat young leaves are used as a food material,
Processing (branching processing) for preventing deterioration (including discoloration such as fading) of the food is performed. For the blanching treatment, various methods are used depending on the form to be treated.For example, hot water treatment, steaming treatment and the like are mentioned, and by this treatment, the material derived from wheat young leaves does not fade. Green color and no change in flavor. However, since GABA is lost in the conventional hot water treatment, a treatment such as microwave treatment that retains GABA and does not cause deterioration (green of wheat young leaves is retained) is preferred.

The hot water treatment is described, for example, in Japanese Patent No. 254430.
It can be performed by the method described in Japanese Patent Publication No. That is, it is performed by immersing young wheat leaves in hot water containing salt and sodium bicarbonate.

The microwave treatment may be performed within a range in which an enzyme involved in fading of young wheat leaves is inactivated and the green color of young wheat leaves is not lost by heating. Such a range can be appropriately adjusted by the output of the microwave irradiation device, the wavelength of the microwave, the irradiation time, and the like. For example, 2450 MHz, 500 g per 100 g (wet weight) of wheat young leaves
When applying a microwave of W, 0.5 to 10
Minutes, preferably 0.5 to 5 minutes, more preferably 0.5 to 5 minutes.
Process for 1 minute. If the time is less than 0.5 minutes, the enzyme is not sufficiently deactivated, and the color is easily discolored. Further, if the treatment is performed for 10 minutes or more, the color tends to fade and the GABA tends to decrease. By microwave treatment, the decomposition and elution of vitamins and minerals in wheat young leaves are prevented, and a dry powder that retains green color can be obtained by drying at a relatively low temperature.

In one embodiment, the barley leaf shreds, juice and extract are treated at normal pressure or under pressure or reduced pressure at 80 to 150 ° C. for 2 to 180 seconds.

The processed product of wheat young leaves obtained in this way may be used as it is, or may be further cut into pieces, squeezed, extracted and used as a food material, or may be further dried and powdered as a food material. .

Dry pulverization is carried out so that the water content is 10% or less, preferably 5% or less, and can be obtained by further pulverizing if necessary.

As the drying method, any drying method such as hot air drying, high pressure steam drying, electromagnetic wave drying, freeze drying, spray drying and the like can be used. Drying should be performed at a temperature as low as possible. Even when heating and drying, for example, 50 to 80
C., preferably 55-65.degree. After adding excipients such as dextrin, cyclodextrin, starch, and maltose, spray drying or freeze drying can also be performed.

The pulverization can be carried out according to a known method, for example, using a crusher, a mill, a blender, a stone mill or the like.

The food material derived from wheat young leaves obtained as described above may be further subjected to, for example,
Sterilization can be performed by any technique known to those skilled in the art, such as autoclaving and heat sterilization.

The wheat young leaf powder is obtained by dry-pulverizing wheat young leaf without performing fragmentation and extraction.

Wheat young leaf juice is obtained by squeezing at any stage of preparing a material derived from wheat young leaves, and this dried powder is wheat young leaf juice powder.

In the raw material derived from wheat young leaves of the present invention, an extract of wheat young leaves may be used. Wheat young leaf extract, at any stage after the GABA enrichment treatment, water, wheat, an extraction solvent commonly used by those skilled in the art, such as an ethanol solution, is added and heated and extracted as necessary, This includes the concentrated version. The wheat young leaf extract powder is obtained by drying and powdering the wheat young leaf extract.

In the present specification, the material derived from wheat young leaves prepared so as to retain GABA refers to a food material derived from wheat young leaves that has been processed so that GABA is not lost.

In the present specification, the material derived from wheat young leaves that has been GABA-enriched refers to a food material derived from wheat young leaves that has been GABA-enriched and processed to retain the increased GABA. .

The amount of GABA contained in wheat young leaf powder varies depending on the preparation method and depends on the conventional method (Japanese Patent No. 254430).
The amount of GABA contained in wheat young leaf powder prepared according to No. 2) is at most about 10 mg / 100 g. On the other hand, the wheat young leaf powder prepared to retain GABA in the present invention contains GABA of at least 20 mg / 100 g, and is usually 10 times or more, preferably 20 times or more as compared with the conventional method. More than twice, more preferably 5
0 times or more, that is, usually 100 mg / 100 g or more, preferably 200 mg / 100 g or more, more preferably 5 mg / 100 g or more.
Contains GABA over 00mg / 100g.

The GABA-enriched wheat young leaf powder in the present invention contains at least 50 mg / 100 g or more of GAB.
A, containing more GABA than wheat young leaf powder prepared so as to retain GABA, usually 2 times or more, preferably 5 times or more, more preferably 10 times or more, that is, usually 200 mg / 100 g or more, preferably Is 500mg /
100 g or more, more preferably 1000 mg / 100 g
The above GABA is included.

The amount of GABA contained in the wheat sprouts powder obtained as described above depends on the preparation method, and the content of GABA contained in the wheat sprouts powder obtained according to the conventional method is as follows. , At most about 50 mg / 100 g.

The wheat young leaf juice powder prepared to retain GABA in the present invention contains at least 100 mg / g
It contains 100 g or more of GABA and is usually 5 times or more, preferably 10 times or more, more preferably 20 times or more, that is, usually 250 m
g / 100 g or more, preferably 500 mg / 100 g or more, more preferably 1000 mg / 100 g or more GA
Contains BA.

The GABA-enriched wheat young leaf juice powder according to the present invention contains at least 250 mg / 100 g or more of GABA, and is usually compared with wheat young leaf juice powder prepared to retain GABA. 2 times or more, preferably 5 times or more, more preferably 10 times or more, that is, usually 500 mg / 100 g or more, preferably 1250 mg
/ 100 g or more, more preferably 2500 mg / 100
g or more of GABA. In addition, these GABA contents are hardly affected by the dry pulverization treatment.

The obtained wheat leaf-derived material can be used for food and drink as it is, but may contain excipients, extenders, binders,
It can be mixed with thickeners, emulsifiers, coloring agents, flavors, food additives, seasonings, etc., and formed into powders, granules, tablets, etc. depending on the application. Can be served for eating and drinking.

Further, since the material derived from wheat young leaves produced by the method of the present invention contains GABA at a high concentration, it can be purified as necessary to obtain a pure GABA product.

The obtained wheat leaf-derived material is used as a raw material for health food in combination with other materials. In particular, a combination with another material containing a component having an antihypertensive effect is effective. Food materials containing such an antihypertensive component include, but are not limited to, deciduous plant Eucombeaceae Eucommia (Tochu), milk protein or fish protein hydrolyzate, or fermented lactic acid bacteria Not done.

If these food materials are formulated in combination with a material derived from wheat young leaves, the components naturally contained therein are:
Vitamins, minerals, dietary fiber,
An additive and / or synergistic effect with a component such as GABA can provide an unprecedented food material. Other materials such as those described above that are combined with materials from wheat young leaves are generally commercially available and readily available. Alternatively, according to the disclosure of the present specification, it can be prepared from raw materials using a method generally known to those skilled in the art.

Specifically, the following can be exemplified as a material that can be combined with a food material derived from wheat young leaves.

Tochu leaves and extracts thereof: Tonaka leaves picked before falling are dried, if necessary, until the water content becomes 10% or less, and then powdered and used as they are, or water or ethanol solution is used. In addition, heat extraction is performed.
The obtained extract is concentrated under reduced pressure to obtain Tochu leaf extract. The obtained Tochu leaf extract may be used as it is, may be powdered, or may be glycoside-containing granules. As a method of forming granules, conventional (reduced pressure) concentration to dryness, freeze drying, spray drying, fluidized bed granulation, roll granulation and the like are used. If necessary, Tonaka leaf extract may be concentrated and / or
Alternatively, it is used after being purified. Tochu leaf extract contains geniposidic acid, syringaresinol di
Glycosides include glycosides such as glucoside, pinoresinol di-O-β-D-glucoside, liliodendrin, asperloside and syringin. Tonaka leaves may be used instead of Tonaka leaves.

Milk protein or fish protein hydrolyzate: Here, milk protein or fish protein hydrolyzate refers to a material containing milk protein or fish protein, which is obtained by converting trypsin, pepsin, thermolysin, chymotrypsin, proteinase, carboxypeptidase,
Means obtained by hydrolysis with one or more proteolytic enzymes including pronase and the like.

As a material containing milk protein or fish protein, animal milk, animal milk casein, skim milk powder, or any fish protein including bonito, krill skim protein, sardine muscle, sardine, mackerel and shrimp octopus can be used. Can be Alternatively, JP-A-7-215889
As described in (1), a material containing angiotensin converting enzyme may be used to obtain a degraded fragment of angiotensin II having angiotensin converting enzyme inhibitory activity.

[0050] The material subject to the action of proteolytic enzymes is usually liquid or solid. The solid material is
Usually, water or hot water is added and mixed, homogenized with strong stirring, pretreated, and then degraded by adding a protease. In general, the amount of protease added is 0.01% based on the amount of liquid material (for solid material, based on the total amount of water and solid material used for pretreatment).
It is added in the range of ~ 5.0% by weight. Hydrolysis is generally between 10 and 80, depending on the proteolytic enzyme used.
C., pH 4-9, between 10 minutes and 30 hours. Although the hydrolysis rate is not particularly limited, the final hydrolysis rate is 1
It is prepared to be 50%. From the obtained reaction solution, inactivate or remove the enzyme added by a method known to those skilled in the art, or isolate and powder the produced peptide, or powder as it is, or add various additives. And then water is removed to obtain peptide-containing granules. As a method of forming granules, conventional (reduced pressure) concentration and drying, freeze drying, spray drying, fluidized bed granulation, roll granulation and the like are used.

Fermented product of lactic acid bacteria: Yogurt produced by fermenting lactic acid bacteria using animal milk as a raw material is exemplified. Typically, lactobacillus (Lacto) is produced from milk.
bacillus sp. ), A bacterium belonging to the genus Streptococcus sp. Is inoculated alone or in combination, and cultured at a temperature of 30 to 45 ° C. for several hours to several days. After culturing, it can be used as it is or after homogenization treatment.

Other food materials that can be combined with wheat wheat leaf-derived materials include one of the above-mentioned food materials, if necessary.
It is selected from species or more and formulated in combination. Wheat young leaf-derived material is a mixture of natural wheat young leaf containing vitamins, minerals, dietary fiber, etc., in an amount sufficient to perform their functions with one or more selected food materials. Is done. The selected one or more food ingredients are mixed with ingredients from wheat young leaves in a form known to those skilled in the art, usually in powder form. Royal jelly,
It contains vitamins, protein, calcium, chitosan, lecithin, etc., and can be added with sugar solution and seasonings to adjust the taste. These can be provided as liquid foods depending on the application or preference. Or capsules such as hard capsules and soft capsules,
It can be formed as a tablet or pill, or into a powder, granule, tea, tea bag or candy shape. These may be eaten as they are, or may be dissolved in water, hot water or milk, and may be drunk, or may be drunk after leaching the components, depending on their shape or taste. The mixing ratio of the wheat leaf material and the selected food material is usually in the range of 1: 9 to 9: 1 (weight ratio), preferably in the range of 1: 4 to 4: 1 (weight ratio). .

[0053]

EXAMPLES The present invention will be described below with reference to examples.
It goes without saying that the present invention is not limited by this embodiment.

1. Preparation of Wheat Young Leaf-Derived Material The wheat young leaf powder and wheat young leaf juice powder used in the present invention were prepared as follows, and the GABA content contained in each was measured using an amino acid automatic analyzer under the following conditions. did.

(Operation conditions of amino acid automatic analyzer) Model: JLC-500 / V (JEOL Ltd.) Column: LCR-6, 4 mm × 90 mm (JEOL Ltd.) Mobile phase: lithium citrate buffer (JEOL Ltd.) Company) P-21 (pH 2.98, Li 0.105 mol / 1) 0 → 16.3 min P-12 (pH 3.28, Li 0.26 mol / 1) 16.3 → 36.1 min P-13 (pH 3.46, Li 0.80 mol / 1) 36.1 → 56.0 min P-14 (pH 2.83, Li 1.54 mol / 1) 56.0 → 63.4 min P-15 (pH 3.65, Li 1.54 mol / 1) 63.4 → 80.0 min Reaction solution: Ninhydrin / hydridantine TS (Wako Pure Chemical Industries, Ltd.) Company) Temperature: Column 35 ° C (0 → 16.3 min), 64 ° C (15.3 → 31.0 min) 44 ° C (31.0 → 44.4 min), 72 ° C (63.4 → 80.0 min) Reaction vessel 135 ° C Flow rate: 0.50 ml of mobile phase / min Reaction liquid 0.30m1 / min Measurement wavelength: 570nm

(Preparation of young barley leaves (conventional method)) about 30 cm
The young leaves of the barley that had grown to a minimum were picked, washed with water, drained, and cut to a length of about 10 cm. 100 g of the cut barley leaves are treated with a conventional method for treating wheat leaves (Patent No. 254).
No. 4302). That is, 100 g of young barley leaves were placed in 1 liter of hot water (95 ° C.) containing 7.5 g of sodium chloride and 7.5 g of sodium bicarbonate, and treated with hot water for 3 minutes. The hot water-treated wheat young leaves were immediately transferred to cold water at 2 to 7 ° C and immersed for about 5 minutes to cool. After cooling, the mixture was centrifuged for about 45 seconds to dehydrate and dried in a dryer at 60 ° C. for 6 hours so that the water content was 5% or less. This is crushed with a stone mill and 2
The powder was crushed so that 90% of the powder passed through a 00 mesh to obtain a young barley powder. The obtained wheat young leaf powder contained about 8 mg of GABA per 100 g.

(Preparation of Barley Juicy Juice Powder (Conventional Method)) Barley young leaf juice powder was prepared according to a conventional method. Barley young leaves were cut and arranged in the same manner as in the conventional method, treated with 1 liter of hot water containing 7.5 g of sodium chloride and 7.5 g of sodium bicarbonate, immediately immersed in water at 2 to 7 ° C. for 5 minutes, and cooled. After cooling, about 4
Centrifuged for 5 seconds to dehydrate. Then, the mixture was pulverized and squeezed by a mixer and filtered to obtain a squeezed juice from which the fiber content was removed. This juice was freeze-dried to obtain a juice powder. The obtained barley young leaf juice powder contained 42 mg / 100 g of GABA.

(Preparation of Barley Young Leaf Powder (Test Example)) Barley young leaves cut and arranged in the same manner as in the conventional method were put into a plastic bag, air was evacuated, and then nitrogen was filled. This was placed in an incubator at 40 ° C. for 6 hours and subjected to anaerobic treatment (GABA enrichment treatment). Next, the wheat young leaves that had been GABA-enriched were subjected to microwave treatment for 1 minute using a microwave oven (RE-121 manufactured by Sharp). This was dried in a drier at 60 ° C. for 6 hours so that the water content was 5% or less, and further crushed with a stone mill so that 90% of 200 mesh passed through to obtain barley young leaf powder.

[0059] Barley young leaves not subjected to the GABA enrichment treatment were similarly treated to obtain barley young leaves. In the obtained wheat young leaf powder, about 250 mg per 100 g of wheat young leaf powder without GABA enrichment treatment was added.
The enriched one contained about 1350 mg of GABA.

(Preparation of Barley Juvenile Juice Powder (Test Example)) Similar to the preparation of barley young leaf powder, barley young leaves that have been subjected to GABA enrichment treatment or not have been subjected to microscopic treatment for 1 minute so that GABA is retained. Wave processed. Next, the mixture was pulverized with a mixer, and further centrifuged and filtered to obtain a juice from which the fiber content was removed. This juice was freeze-dried to obtain a juice powder. The obtained barley young leaf juice powder contained about 600 mg of GABA per 100 g without GABA enrichment and about 3000 mg of GABA with GABA enrichment.

[0061] 2. Preparation of a material containing a component having an antihypertensive action other than GABA A material containing a component having an antihypertensive action other than GABA used in the present invention is prepared as follows, and if necessary, an antihypertensive action is prepared. Angiotensin converting enzyme inhibitory activity (hereinafter, referred to as ACE inhibitory activity) known in the art was measured as one index having the following.

(Measurement of ACE inhibitory activity)
ACE inhibitory activity was measured as described below according to the method described in Japanese Patent No. 70533.

1 g of langacetone powder (rabbit) was added to 10 ml of 0.1 M borate buffer (pH-
After stirring for 2 hours in 8.3), the mixture was centrifuged to precipitate insolubles, and the supernatant was filtered (0.2 μm) to prepare an enzyme solution. Then, using the same borate buffer, a substrate solution containing 5 mM hypril-L-histidyl-L-leucine and 300 mM sodium chloride was prepared. Next, 250 μl of the substrate solution was added to 30 μl of the gel-filtered fraction, stirred, and then incubated at 37 ° C. for 10 minutes. To this, add a 5-fold dilution of the enzyme solution to 100
After adding 30 μl of the enzyme reaction at 37 ° C. for 30 minutes,
The enzyme reaction was stopped by adding 50 μl of 1N hydrochloric acid. Further, 1500 μl of cold ethyl acetate was added, and the mixture was stirred and centrifuged.
Heat at 20 ° C. for 30 minutes to dry the lysate. The obtained dried product was dissolved in 1,000 μl of distilled water,
The absorbance at 8 nm was measured. A blank to which a borate buffer was added was used as a blank. The inhibition rate was calculated by (absorbance when using blank-absorbance when adding sample) / (absorbance when using blank), and the concentration at 50% inhibition was calculated as IC
₅₀ was set.

(Preparation of Hydrolyzate of Milk Protein) A hydrolyzate of milk protein was prepared according to the method described in JP-A-62-270533. Briefly, casein from milk was suspended in 50 mM phosphate buffer (pH-7.4) to prepare a 4% solution (50 ml). Then, trypsin was added to the suspension to a final concentration of 0.1%, and the mixture was incubated overnight at 37 ° C. with gentle stirring. After the reaction, concentrated hydrochloric acid was added to denature trypsin and undegraded casein. The insoluble material was sedimented by centrifugation, and the supernatant was recovered. The obtained supernatant was lyophilized and concentrated, and then washed with water Sephadex G-2.
5 and fractionated by gel filtration chromatography. ACE for each fraction as follows
The inhibitory activity was measured, and the active fraction was collected. This was freeze-dried, fractionated again, the active fraction was collected, and the freeze-dried dried product was used as a milk protein hydrolyzate to be mixed with feed. The ACE inhibitory activity of the milk protein hydrolyzate thus obtained was IC ₅₀ = 1.8 mg / ml.

(Preparation of Fish Protein Hydrolyzate) A fish protein hydrolyzate was prepared according to the method described in JP-A-7-188282. Briefly, fresh sardines were minced and minced. After adding an equal volume of water to 50 g of sardine surimi, the pH was adjusted to 2.0 using a 1N hydrochloric acid solution. Then, pepsin was added to a final concentration of 1%, and the mixture was incubated at 37 ° C. for 20 hours with stirring. The reaction solution was immediately subjected to ultrafiltration (filtration membrane; YM-10, manufactured by Amicon),
The filtrate passed through the filtration membrane was applied to a Dowex 50W × 4 (H ⁺ ) column. Next, after the column was thoroughly washed with distilled water, the adsorbed components were eluted with 2N ammonium hydroxide. The obtained eluate was concentrated under reduced pressure, passed through Sephadex G-25 previously washed with distilled water, and fractionated. The ACE inhibitory activity was measured for each fraction, and the active fraction was collected and lyophilized to obtain a concentrated solution. The gel fraction was again filtered to collect the active fraction, and the freeze-dried product was used as a fish protein hydrolyzate to be mixed with feed. The ACE inhibitory activity of the fish protein hydrolyzate thus obtained was IC ₅₀ = 2.1 mg / ml.

(Preparation of Tochu leaf extract) Patent No. 2579
An extract of Tonaka leaf was prepared according to the method described in JP-A-255. To sum up, the Tochu leaves picked before they fall,
It was dried at 60 ° C. for 6 hours so that the water content was 5% or less. Next, 500g of dried 100g Tonaka leaves
Extracted in 100 ml of distilled water for 30 minutes at 100 ° C. The extracted solution was freeze-dried to obtain a Tochu leaf extract to be mixed with feed.

(Preparation of Fermented Product of Lactic Acid Bacteria)
A fermentation product was prepared according to the method described in JP-A-003228. 1 g of fermented milk (3.5 × 10 ⁸ cells / ml) obtained by inoculating Lactobacillus helveticus in a 9% by weight aqueous solution of skim milk powder sterilized at 115 ° C. for 20 minutes was added to 1
It was inoculated into 00 ml of a 9% by weight aqueous solution of skim milk powder sterilized at 90 ° C. and cultured at 37 ° C. for 24 hours. The obtained culture was freeze-dried to obtain a fermented product to be mixed with feed.

3. Antihypertensive Test Using Spontaneously Hypertensive Rats (SHR) A material containing the anti-hypertensive component other than GABA prepared as described above and the wheat barley leaf powder or wheat young leaf juice powder prepared as described above is shown in Table 1. The suspensions in a 0.5% methylcellulose solution having the composition (weight ratio) shown in No. 1 were used as feeds 1 to 35, respectively. The unit of the numerical values shown in Table 1 is% by weight.

[0069]

[Table 1]

The feeds 1 to 24 shown in Table 1 are feeds containing the food material of the present invention containing wheat young leaf powder or wheat young leaf squeezed powder and a material containing an antihypertensive component other than GABA. It is an example showing the composition. Feed 25 ~
Numeral 34 is a comparative example containing wheat young leaf powder, wheat young leaf squeezed powder, casein hydrolyzate, fish protein hydrolyzate, Tochu leaf extract, or a fermented product of lactic acid bacteria alone.

These diets were administered to 9-week-old spontaneously hypertensive rats (SHR) consisting of 6 rats in each group using a gastric tube.
Oral administration was performed at 0 ml / Kg body weight, and blood pressure was measured 5 hours later. Table 2 shows the results of the blood pressure difference at the start of feed administration and 5 hours after the start of the administration of each group. In Table 2, the blood pressure at the time of the test was the average value of the blood pressure measured for 6 animals in each group at the start of the feed administration, and the blood pressure after 5 hours was the blood pressure measured for 6 animals in each group 5 hours after the food administration. The mean value, and the blood pressure difference of each group were the mean value of the blood pressure at the start of the test,
This is the difference from the average value of the post-hour blood pressure.

[0072]

[Table 2]

As shown in Table 2, feeds 1 to 24 containing the food material of the present invention containing wheat young leaf powder or wheat young leaf juice powder and a material containing an antihypertensive component other than GABA were used. In the group to which the food was administered, the blood pressure was reduced in the range of 18.9 mmHg (feed 2) to 42.3 mmHg (feed 24) as compared to the start of the feed administration at an average value 5 hours after the feed administration. In a group to which feeds 25 to 34 each containing a young leaf powder, wheat young leaf juice powder, casein hydrolyzate, fish protein hydrolyzate, Tochu leaf extract, or a fermented product of lactic acid bacteria alone were administered, 4.0 mmHg (feed) 2
5) Only reduced blood pressure in the range of -14.8 mmHg (feed 30).

The results show that the material derived from wheat young leaves and GABA
Feed containing a combination of ingredients with anti-hypertensive action other than the above is more excellent in anti-hypertensive effect than containing each ingredient alone, and this effect is obtained from wheat young leaves that retain GABA To be enhanced by being prepared or enriched.
That is, it shows that a synergistic anti-hypertensive effect was obtained by combining the material derived from wheat young leaves of the present invention with another material containing an anti-hypertensive component other than GABA.

[0075]

The present invention provides a novel food material containing γ-aminobutyric acid, vitamins, minerals, dietary fiber and the like in high concentrations.

Claims (6)

[Claims] 1. A food material comprising a material derived from wheat young leaves and a material containing a component having an antihypertensive effect other than GABA. 2. The food material according to claim 1, wherein the wheat leaf-derived material has been subjected to GABA enrichment treatment. 3. The component having an antihypertensive action other than GABA is an angiotensin converting enzyme inhibitory component.
The food material according to claim 1. 4. The food material according to claim 1, wherein the material containing a component having an antihypertensive action other than GABA is a hydrolyzate of milk protein or fish protein or a fermented product of lactic acid bacteria. 5. The food material according to claim 1, wherein the material containing a component having an antihypertensive effect other than GABA is Tochu leaf or an extract thereof. 6. The food material according to claim 1, wherein the component having an antihypertensive effect other than GABA is a peptide, a glycoside, or geniposidic acid.