JP3409034B2 - Method for producing cruciferous plant and processed product thereof - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a Brassicaceae plant rich in γ-aminobutyric acid (hereinafter sometimes referred to as GABA). More specifically, a cruciferous plant having a green color and having a high content of GABA, its juice,
The present invention relates to those dry powders, their production methods, their foods, beverages, and pharmaceutical raw materials.

[0002]

2. Description of the Related Art Since GABA has a blood pressure lowering action, foods containing a large amount of GABA have been studied for people with hypertension. For example, conventionally, in the process of manufacturing tea, the plucked tea leaves are placed under anaerobic conditions and GAB is added to the tea leaves.
A so-called Gabalon tea in which a large amount of A is accumulated is known. Japanese Unexamined Patent Publication No. 8-173111 describes that coffee leaf tea having a high GABA concentration is obtained by a method of treating fresh coffee leaves in an oxygen-free state, followed by heat treatment and drying treatment at a high temperature of 110 ° C. or higher. . Furthermore, JP-A-9-
No. 205989 discloses that GA is obtained by irradiating tea leaves with infrared rays.
It is described to improve the content of BA.

The tea leaves obtained by these methods are obtained by pouring hot water into the extract, and the useful ingredients that can be ingested are limited to those soluble in water.

On the other hand, cruciferous plants such as kale contain a large amount of components such as vitamins, and are attracting attention as health foods. Cruciferous plants such as kale are squeezed or made into a dry powder and used for drinking and eating. Also, cruciferous plants, dry powder,
In order to maintain the green color of the juice and the dried powder of the juice, so-called blanching treatment is generally performed in which the cruciferous plant is treated at a high temperature such as hot water. However, with hot water treatment, there is a high possibility that the active ingredient will disappear, and it is not possible to obtain a cruciferous plant that maintains the active ingredient and retains a green color.

For example, a cruciferous plant (for example, cabbage) is shredded, vitamin C is added, and heat treatment is performed.
A method of squeezing juice to obtain juice is disclosed in JP-A-10-4284.
No. 1 publication. However, this method has a problem in that nutrients vulnerable to heat are decomposed.

Further, Japanese Patent No. 2796227 describes a method of obtaining a dry powder of a cruciferous plant by combining preliminary drying for 12 to 13 hours and far infrared rays and treating at a relatively low temperature. . However, the conventional method has a drawback that only the nutrients possessed by cruciferous plants such as kale are edible. Therefore,
When a nutrient or a component having a certain medicinal effect (for example, GABA) is further added to the cruciferous plant, a large amount of the active ingredient (for example, GAB) is directly added instead of the above extraction.
Since A) can be ingested, further effects as a health food are expected.

[0007]

Therefore, there is a demand for a dry powder, a squeezed powder, and a squeezed dry powder of cruciferous plants to which new nutrients or medicinal components are added.

[0008]

Means for Solving the Problems The present inventor has conducted intensive studies on cruciferous plants, and found that cruciferous plants contain GABA having a hypotensive action,
Then, they succeeded in increasing the GABA content in the cruciferous plant, and completed the present invention. Brassicaceae plants are particularly suitable for the treatment for increasing the GABA content, and it was possible to obtain cruciferous plants with an increased GABA content.

According to the present invention, a Brassicaceae plant having an increased GABA content is provided, and the Brassicaceae plant having an increased GABA content is directly used for food. Also,
By drying the cruciferous plant having the increased GABA content, a dry cruciferous plant powder containing a large amount of GABA is provided. Further, by squeezing a Brassicaceae plant having an increased GABA content, a squeezed juice rich in GABA is provided, and further a squeezed dry powder rich in GABA is provided. Accordingly, in addition to the conventional cruciferous plant juice and dry powder, there is provided a health food having a new added value of having a blood pressure lowering action.

In the present invention, GABA is 50 mg / 100 g
The present invention relates to a Brassicaceae plant.

In a preferred embodiment, the cruciferous plant retains a green color.

The present invention is branching processed and
10mg / 100g of γ-aminobutyric acid which keeps green color
The present invention relates to a Brassicaceae plant or squeezed juice thereof.

The present invention also provides a method for producing a cruciferous plant containing GABA in an amount of 50 mg / 100 g or more, which comprises a step of subjecting the cruciferous plant to anaerobic treatment and / or heat retention treatment at 25 to 50 ° C. Regarding

In a preferred embodiment, the method further includes the step of performing a microwave treatment.

[0015] In a preferred embodiment, the treated cruciferous plant retains a green color.

In the present invention, GABA is 50 mg / 1.
The present invention relates to squeezing of cruciferous plants containing at least 00 g.

[0017] In a preferred embodiment, the juice of the cruciferous plant retains a green color.

The present invention further comprises a step of subjecting the cruciferous plant to an anaerobic treatment and / or a heat-retaining treatment at 25 to 50 ° C., and squeezing the treated cruciferous plant to 50 mg of GABA / The present invention relates to a method for producing juice of a cruciferous plant containing 100 g or more.

Furthermore, the present invention includes the step of anaerobically treating and / or heat-retaining at 25 to 50 ° C. for squeezing a cruciferous plant, GABA of 50 mg / 100.
The present invention relates to a method for producing a juice of a cruciferous plant containing g or more.

In a preferred embodiment, the method for producing juice as described above further includes a step of performing a microwave treatment.

In a preferred embodiment, the juice obtained retains a green color.

The present invention also provides GABA at 180 mg /
It relates to a dry powder of a cruciferous plant containing 100 g or more.

In addition, the present invention provides GABA comprising 1 step of GABA including a step of anaerobic treatment and / or a heat retention treatment at 25 to 50 ° C. for a Brassicaceae plant, and a drying step.
The present invention relates to a method for producing a dry powder of a cruciferous plant containing 80 mg / 100 g or more.

Further, according to the present invention, GABA is 10 mg /
The present invention relates to a dry powder of a cruciferous plant having a green color, containing 100 g or more.

Further, in a preferred embodiment, vitamin B1 is further contained in an amount of 0.1 mg / 100 g or more and vitamin C is contained in an amount of 100 mg / 100 g or more.

The present invention further comprises the step of subjecting the cruciferous plant to a microwave treatment, and the step of drying the treated cruciferous plant to GABA 10
The present invention relates to a method for producing a dry powder of a cruciferous plant that retains a green color, containing at least 100 mg / 100 g.

[0027] In a preferred embodiment, the method further comprises a step of performing anaerobic treatment and / or heat retention treatment at 25 to 50 ° C.

In the present invention, the step of subjecting the cruciferous plant to anaerobic treatment and / or the heat retention treatment at 25 to 50 ° C., the step of subjecting the treated cruciferous plant to the blanching treatment, and the blanching treatment 10 mg / 1 of γ-aminobutyric acid including a step of drying cruciferous plants
The present invention relates to a method for producing a dry powder of a cruciferous plant that retains a green color, containing at least 00 g.

In the present invention, GABA is 180 mg /
The present invention relates to a dry powder of cruciferous juice containing 100 g or more.

Further, the present invention provides a step of anaerobically treating and / or heat-retaining the cruciferous plant at 25 to 50 ° C., a step of squeezing the treated cruciferous plant, and the obtained pressing. GAB including the step of drying the juice
The present invention relates to a method for producing a dry powder of squeezed cruciferous plant, which contains 180 mg / 100 g or more of A.

In the present invention, GABA is 10 mg / 1.
It relates to a dry powder of squeezed from a cruciferous plant which retains a green color and contains at least 100 g.

[0032] In a preferred embodiment, the dried powder of the cruciferous squeezed plant which retains the green color further comprises:
Vitamin B1 0.1mg / 100g or more, vitamin C
100 mg / 100 g or more.

The present invention comprises a step of subjecting a cruciferous plant to a microwave treatment, a step of squeezing the treated cruciferous plant, and a step of drying the obtained juice. Contains more than
The present invention relates to a method for producing a dry powder of juice of a cruciferous plant that retains a green color.

Further, the present invention comprises a step of subjecting the juice of the cruciferous plant to microwave treatment, and a step of drying the obtained juice so that GABA is 10 mg / 1.
The present invention relates to a method for producing a dry powder of squeezed from a cruciferous plant that retains a green color, containing at least 00 g.

The present invention also provides a step of anaerobically treating and / or heat-retaining the cruciferous plant at 25 to 50 ° C., a step of blanching the treated cruciferous plant, and a branching treatment. A method for producing a dry powder of squeezed Brassicaceae plant containing 10 mg / 100 g or more of γ-aminobutyric acid, which comprises a step of squeezing the above-mentioned Brassicaceae plant and a step of drying the obtained juice Regarding

Further, according to the present invention, a step of anaerobically treating and / or a heat-retaining treatment at 25 to 50 ° C. on the juice of the cruciferous plant, and a step of blanching the treated cruciferous juice And a step of drying,
The present invention relates to a method for producing a dry powder of squeezed green plant of Brassicaceae, which contains 10 mg / 100 g or more of γ-aminobutyric acid.

The present invention also relates to a method for increasing the GABA content of cruciferous plants, which comprises a step of anaerobically treating and / or heat-retaining cruciferous plants at 25 to 50 ° C.

[0038] The present invention further relates to a method for increasing the GABA content of cruciferous plant juice, which comprises a step of anaerobically treating and / or heat-retaining the cruciferous plant juice at 25 to 50 ° C.

[0039] The present invention also provides a GABA of a cruciferous juice, comprising a step of anaerobically treating a cruciferous plant and / or a heat treatment at 25 to 50 ° C, and a step of squeezing the treated cruciferous plant. It relates to a method of increasing the content.

The present invention further relates to a method for retaining the green color of cruciferous plants, their juices or their dry powders, which comprises the step of subjecting the cruciferous plants or their juices to microwave treatment.

Further, the present invention relates to a Brassicaceae plant having an increased GABA content, squeezing of a Brassicaceae plant having an increased GABA content, a dry powder of a Brassicaceae plant having an increased GABA content, and a rape having a green color. , A beverage, or a pharmaceutical raw material, which contains either a dry powder of a family of plants, a dry powder of a juice of a brassica plant having an increased GABA content, or a dry powder of a juice of a brassica plant that retains a green color. Regarding

[0042] In a preferred embodiment, the cruciferous plant is kale, the cruciferous juice is kale juice, and the cruciferous dry powder is kale dry powder. The dried powder of juice is the dried powder of kale juice.

[0043]

BEST MODE FOR CARRYING OUT THE INVENTION Examples of the cruciferous plant used in the present invention include cabbage, broccoli, cauliflower, kale, radish, wasabi, komatsuna and the like.
Of these, kale is particularly preferable. The term "Brassicaceae" includes a case where a part of leaves, stems, flowers, etc. is mentioned. Furthermore, the term "Brassicaceae plant" includes a fragment of a Brassicaceae plant.

The cruciferous plant is fragmented by a method commonly used by those skilled in the art, such as slicing or shredding, to fragment the plant to obtain a fragment. Some of the fragments are in the form of a slurry, but the slurry is a juicer,
It is processed by a blender or the like.

The Brassicaceae plants and the like containing GABA in a specific amount or more according to the present invention were completed by subjecting the Brassicaceae plants to a treatment for increasing the GABA content (hereinafter referred to as GABA enrichment treatment). Hereinafter, the GABA enrichment process will be described first.

(GABA Enrichment Treatment) G of Brassicaceae
The ABA enrichment treatment is applied to the cruciferous plant, preferably to the cut, shredded or fragmented cruciferous plant.
Anaerobic treatment, heat retention treatment at 25 to 50 ° C, or both are performed at the same time.

Anaerobic treatment means treatment with a gas containing little or no oxygen. Including vacuum state. Carbon dioxide gas and nitrogen gas are preferably used as the gas.

The method of heat retention treatment does not matter. Examples include hot water treatment, infrared irradiation treatment, warm air treatment, incubator treatment and the like. Infrared irradiation treatment is preferably used.

The time for the anaerobic treatment and / or the heat retention treatment is preferably 30 minutes to 24 hours, more preferably 1 to 6 hours. The temperature of anaerobic treatment and / or heat retention treatment is about 2
5-50 degreeC is preferable, about 30-45 degreeC is more preferable, about 40 degreeC is the most preferable. Is it below 25 ° C?
When the temperature exceeds 50 ° C, the GABA content is hard to increase.

The GABA treatment also includes storage and storage of cruciferous plants in a constant temperature room at a constant temperature (for example, 25 to 50 ° C.).

The anaerobic treatment and / or the heat retaining treatment is preferably carried out under the condition that the Brassicaceae plant is not dried, but it may be dried.

The above anaerobic treatment and / or heat retention treatment (G
GABA of the cruciferous plant obtained by ABA enrichment treatment)
The content is increased. For example, as shown in Table 1 of Example 1 of the present invention, broccoli can increase the GABA content 3.6 times, cabbage about 6 times, and kale about 6.2 times.

(Brassicaceae Plant Containing 50 mg / 100 g or More of GABA) 50 mg / gap of GABA of the Present Invention
The Brassicaceae plant containing 100 g or more is obtained by the GABA enrichment treatment.

Generally, the GABA content of cruciferous plants is
The highest amount of kale is around 30mg / 100g,
Brassicaceae plants containing GABA in an amount of 50 mg / 100 g or more have never existed before and are novel. The GABA-enriched cruciferous plant of the present invention,
In particular, the GABA content of kale is preferably 80 mg / 100 g or more, more preferably 100 mg / 100 g or more, further preferably 200 mg / 100 g or more, and further 250 mg / 100 g or more.

Regarding the GABA content, when referring to cruciferous plants and squeezed juice, the weight is based on wet weight, and when referring to the cruciferous plant dry powder and squeezed dry powder, the weight is based on dry weight. Say.

(Squeezing of Brassicaceae Plant Containing 50 mg / 100 g or More of GABA) GABA of the present invention is 50
Juicing of cruciferous plants containing more than mg / 100 g is
As for the cruciferous plant, preferably the cut, shredded or shredded cruciferous plant, GABA is used in the same manner as above.
It is obtained by performing an enrichment treatment, then pressing the GABA-enriched cruciferous plant, and if necessary, applying a method commonly used by those skilled in the art, such as filtration or centrifugation. In addition, the expression "squeezed juice" as used herein is meant to include an extract, and includes green juice obtained by pressing, an ethanol extract from a cruciferous plant, and a hot to hot water extract of a cruciferous plant.

The GABA of the present invention was added in an amount of 50 mg / 10.
The squeezing of the cruciferous plant containing 0 g or more is performed by first squeezing the cruciferous plant, preferably a cut, shredded or fragmented cruciferous plant, and if necessary, a person skilled in the art such as filtration or centrifugation. Can also be obtained by applying the method usually used to obtain the squeezed juice of the cruciferous plant and subjecting the squeezed juice to the GABA enrichment treatment in the same manner as described above.

The above anaerobic treatment and / or heat retention treatment (G
GABA of crucifer squeezed by ABA enrichment treatment)
The content is increased. Usually, the GABA content of the cruciferous plant is the highest in kale, and is about 30 mg / 100 g, so that the squeezing of the cruciferous plant containing GABA of 50 mg / 100 g or more is one that has not existed until now. It is new. The GABA content of the GABA-enriched cruciferous plant of the present invention, particularly the kale juice, is 8
0 mg / 100 g or more is preferable, 100 mg / 100
g or more is more preferable, 200 mg / 100 g or more is still more preferable, and further 250 mg / 100 g can be used.

The cruciferous plant which is blanching-treated and contains 10 mg / 100 g or more of γ-aminobutyric acid retaining a green color can be obtained by subjecting the above-mentioned GABA-enriched cruciferous plant to a blanching treatment.
Cruciferous plant juice containing 10 mg / 100 g or more of blanching-treated and γ-aminobutyric acid that retains green color is GABA-enriched, and then blanched plants are squeezed or GABA-enriched. It is obtained by blanching the juice. Most GABA disappears by blanching treatment (5 mg / 10
(Less than 0 g), GABA enrichment treatment and blanching treatment give 10 mg / 10
0 g or more of GABA remains.

(Dry powder of Brassicaceae containing 180 mg / 100 g or more of GABA) 18 GABA of the present invention
The dry powder of the cruciferous plant containing 0 mg / 100 g or more is obtained by drying the above-mentioned GABA-enriched cruciferous plant. GAB of Brassicaceae
The kale content is the highest in kale, which is about 30 mg / 100 g, and when it is dried, the GABA content is about 170.
It is mg / 100g. Therefore, the GABA-enriched plant of the family Cruciferae was dried to obtain 180 GABA.
The cruciferous plant dry powder containing not less than mg / 100 g is novel because it has not existed until now.
In addition, a dry powder having a required GABA content can be obtained by appropriately selecting a GABA enrichment treatment method and conditions. For example, 300mg / 100g or more, 40
0mg / 100g or more, 500mg / 100g or more, 1
It is possible to obtain a dry powder having a GABA content of 000 mg / 100 g or more and 1500 mg / 100 g or more. As an upper limit, about 4000 mg / 100 g of dry powder can be obtained.

When the cruciferous plant is dried, the water content is 10%.
Hereinafter, it is preferably performed so as to be 5% or less. As the drying method, a method that does not raise the temperature so much, such as warm air drying, far infrared drying, electromagnetic wave drying, and freeze drying, is preferable from the viewpoint of preservation of nutrients. Drying is preferably performed at a temperature as low as possible (for example, 50 ° C. or lower), and even when drying by heating,
For example, it may be carried out at 50 to 80 ° C, preferably 55 to 65 ° C.

When the Brassicaceae plant is dried, an alkaline aqueous solution may be attached to the Brassicaceae plant before the treatment in order to prevent fading of green color.

The dried product is further pulverized to obtain a dry powder. For the pulverization, a method usually used by those skilled in the art is adopted. For example, it is crushed by a crusher, a mill, a blender, a stone mill or the like. If necessary, the dried cruciferous plant crushed product may be sieved and passed through a 30 to 250 mesh to be a cruciferous plant dry powder. If the particle size is smaller than that of 250 mesh, it is difficult to handle during the further processing of the cruciferous dry powder,
If the particle size is larger than that of 30 mesh, the uniform mixing of the cruciferous plant dry powder with other food materials may be hindered.

(Dry powder of crucifer juice containing 180 mg / 100 g or more of GABA) GABA of the present invention
The dried powder of cruciferous juice containing 180 mg / 100 g or more of the above must be squeezed of the above-mentioned GABA-enriched cruciferous plant or GABA-enriched juice of the cruciferous plant, and then dried. Is obtained by Normally, the GABA content of cruciferous plants is the highest in kale, about 30 mg / 100 g, and when this juice is dried, the GABA content is about 170 mg / 100 g. Therefore, the squeezed juice from GABA-enriched cruciferous plants or the dried powder of GABA-enriched squeezed juice containing GABA of 180 mg / 100 g or more, which has been obtained by drying GABA-enriched juice, It did not exist, and is new.

In the case of cruciferous plants, especially kale, G
By appropriately selecting the ABA enrichment treatment method and conditions,
The amount of GABA contained in the dry powder of juice is 180 mg.
It can be ~ 4000 mg / 100 g. Preferably 50
0 mg / 100 g or more, more preferably 800 mg / 1
The amount is 00 g or more, more preferably 1000 mg / 100 g or more, and most preferably 1500 mg / 100 g or more. In a preferred case, the dried powder of kale juice contains 6 times more GABA than the dried powder of conventional juice.

As a method for drying the juice, spray drying or freeze drying is preferable. At the time of drying, from the viewpoint of maintaining the green color, the pH of the juice may be adjusted in advance to a range of 6 to 9 by a means such as adding an alkaline aqueous solution to the juice, and then dried. In addition, an excipient such as dextrin, cyclodextrin, starch, maltose or the like may be added, if necessary, upon drying.

Although the above cruciferous plants, cruciferous juices, cruciferous dry powders, and cruciferous juices dry powders described above can retain a green color by using an alkali or the like. , Tend to cause a change in taste. Usually, when producing dry powders of cruciferous plants, in order to inactivate enzymes etc. involved in alteration (discoloration of green, change of flavor), generally at high temperature such as hot water treatment or steaming treatment. A so-called branching process is performed. However, it was found that GABA disappeared in the branching treatment.

Therefore, the inventors of the present invention have examined the alternative to the conventional blanching treatment in which GABA is less likely to decrease,
It was found that the objective can be achieved by the microwave treatment. In addition, after performing GABA enrichment treatment, it was found that a desired amount of GABA remained by performing a blanching treatment, and a cruciferous plant that retained a green color,
It has been found that the squeezed juice or these dry powders described below can be obtained.

(Microwave Treatment) The microwave irradiation treatment mainly aims to inactivate an enzyme that discolors the green appearance of cruciferous plants. On the other hand, excessive microwave irradiation treatment may cause loss of nutrients such as GABA and vitamins contained in Agarnetaceae plants and active ingredients. Therefore, it is desirable to irradiate the microwave within a range in which the enzyme involved in fading is inactivated and the green color can be retained and the effective component is not lost by the excessive irradiation. Such a range may be appropriately set based on the output of the irradiation device, the wavelength of the microwave, the irradiation time, the type and amount of the cruciferous plant.

For example, when a microwave of 2450 MHz and 500 W is applied, it is 0.5 to 10 minutes, preferably 0.5 to 5 minutes, and more preferably 0. 5 minutes per 100 g (wet weight) of the cruciferous plant kale. Process for 5 to 1 minute. If it is less than 0.5 minutes, inactivation of the enzyme is insufficient and fading easily occurs. If treated for 10 minutes or longer, the active ingredient may decrease. However, even if it is decreased, as shown in the examples described later, it is much more GA than the hydrothermal treatment.
The BA content is high and the green color is retained.

The blanching treatment includes a treatment at a high temperature generally performed by those skilled in the art, such as the above-mentioned hot water treatment or steaming treatment. If blanching is carried out by adding an appropriate amount of sodium bicarbonate or sodium chloride alone or in combination at the time of treatment, the green color is maintained vividly. Of course, there is no problem even if the blanching process and the microwave process are combined.

(Brassicaceae plant that retains green color and contains 10 mg / 100 g or more of GABA)
The cruciferous plant that retains a green color and contains mg / 100 g or more is obtained by subjecting the cruciferous plant to microwave treatment. Alternatively, it can be obtained by a microwave treatment after GABA enrichment treatment. In the blanching treatment described below for maintaining the green color of the cruciferous plant, the amount of GABA contained in the cruciferous family is about 2 mg / 100 g, whereas if the microwave treatment is adopted, GABA is 10 mg / 100
It is possible to obtain a cruciferous plant having a green color, which is contained in an amount of g or more, preferably 20 mg / 100 g or more. Then, by further combining the GABA enrichment treatment and this microwave treatment, a green-colored cruciferous plant containing 50 mg / 100 g or more of GABA can be obtained.

Conventionally, since the green cruciferous plant contains only about 30 mg / 100 g of GABA as described above, a cruciferous plant which retains a green color and contains GABA of 50 mg / 100 g or more is novel. .

By properly selecting the GABA enrichment treatment method and conditions, it is possible to obtain a Brassicaceae plant containing the required amount of GABA. For example, 70 mg / 1
00g or more, 100mg / 100g or more, 150mg /
It is possible to obtain cruciferous plants having a GABA content of 100 g or more and 200 mg / 100 g or more. Regarding the upper limit, it is possible to obtain a Brassicaceae plant having a GABA content of up to about 500 mg / 100 g.

Since the above GABA enrichment treatment and microwave treatment are relatively gentle treatments, vitamins contained in cruciferous plants, especially vitamin B, are included.
1. Vitamin C is maintained without being lost even after drying. Preferably, vitamin B1 is 0.1 mg / 100
g or more, vitamin C, 100 mg / 100 g or more, can be contained in a cruciferous plant that retains a green color.

Further, the plant of the family Cruciferae, which retains a green color and contains GABA in an amount of 50 mg / 100 g or more, can be obtained by subjecting the plant Cruciferae to the GABA enrichment treatment and then the blanching treatment.

(Squeezing of a green-colored cruciferous plant containing 50 mg / 100 g or more of GABA) GABA
The squeezing of the cruciferous plant having a green color containing not less than 50 mg / 100 g is obtained by combining the cruciferous plant with a GABA enrichment treatment and a microwave treatment, and squeezing the treated cruciferous plant. The microwave treatment may be performed before the GABA enrichment treatment or after the GABA enrichment treatment.

The juice of the cruciferous plant which retains the green color and contains GABA in an amount of 50 mg / 100 g or more can also be obtained by combining the juice of the cruciferous plant with a GABA enrichment treatment and a microwave treatment.

Conventionally, green cruciferous plants contain only about 30 mg / 100 g of GABA as described above. Therefore, juice of green cruciferous plants containing GABA of 50 mg / 100 g or more is not squeezed. It is new.

By appropriately selecting the GABA enrichment treatment method and conditions, it is possible to obtain the juice of the Brassicaceae plant containing the required amount of GABA. For example, 70m
g / 100g or more, 100 mg / 100g or more, 200
GA of mg / 100g or more, 300mg / 100g or more
It is possible to obtain juice of cruciferous plants having a BA content.
Regarding the upper limit, it is possible to obtain juice of Brassicaceae plants having a GABA content of up to about 600 mg / 100 g.

In the same manner as above, vitamin B is preferably used in the juice of the cruciferous plant of the present invention which retains green color.
1 is 0.1mg / 100g or more, vitamin C is 100m
g / 100g or more may be contained.

(Dry powder of a cruciferous plant having a green color, containing GABA in an amount of 10 mg / 100 g or more) GA
A dry powder of a cruciferous plant that retains a green color and contains 10 mg / 100 g or more of BA is obtained by subjecting a cruciferous plant to a microwave treatment. As shown in Examples below, GABA is contained in a green dry powder obtained by subjecting cruciferous plants to hot water treatment at 2 mg / 100 g.
Only included. On the other hand, the dry powder of the cruciferous plant having a green color obtained by the microwave treatment contains GABA in an amount of 10 mg / 100 g or more. Therefore, a dry powder of a cruciferous plant that retains a green color and contains GABA in an amount of 10 mg / 100 g or more is novel.

When the cruciferous plant is kale, GABA
Content can be 20 mg / 100 g, 100 mg /
It may be 100 g or more, and further 150 mg / 100 g
Can be more. Combined with the GABA enrichment process,
200mg / 100g or more, further 500mg / 10
It can be 0 g or more.

As in the above case, 0.1 mg of vitamin B1 was added to the dry powder of the cruciferous plant that retained the green color.
100 g or more and 100 mg / 100 g or more of vitamin C may be contained.

Further, a dry powder of a cruciferous plant which retains a green color and contains GABA in an amount of 10 mg / 100 g or more can be obtained by subjecting a cruciferous plant to a GABA enrichment treatment and then a blanching treatment.

(Dry powder of green tea cruciferous juice containing 10 mg / 100 g or more of GABA) Dry powder of green tea cruciferous juice of 10 mg / 100 g or more of GABA is , A cruciferous plant or its juice is subjected to a microwave treatment. As mentioned above, the green dry powder obtained by subjecting Brassicaceae plants to conventional blanching treatment such as hot water treatment contains only about 2 mg / 100 g of GABA, so that the amount of squeezed juice also exceeds that concentration. It cannot be. Therefore, a dry powder of juice of a cruciferous plant that retains a green color, containing GABA in an amount of 10 mg / 100 g or more is novel.

When the cruciferous plant is kale, the content of GABA in the dried powder of juice can be 20 mg / 100 g, 100 mg / 100 g or more, and further 15
It can be 0 mg / 100 g or more. When combined with the GABA enrichment treatment, it can be 200 mg / 100 g or more, and even 500 mg / 100 g or more.

In the same manner as above, vitamin B1 was added to the dry powder of the juice of the cruciferous plant that retained the green color in an amount of 0.1
mg / 100g or more, vitamin C 100mg / 100
g or more may be included.

Furthermore, the dried powder of cruciferous juice that retains a green color and contains GABA in an amount of 10 mg / 100 g or more is obtained by subjecting the cruciferous juice to GABA enrichment treatment and then to blanching treatment. It can also be obtained by drying.

(Use of Brassicaceae Plants Having a High GABA Content) The GABA-rich plants obtained in the present invention, their dry powders, squeezed juices, and dried powders of squeezed juices can be used by those skilled in the art as needed. Commonly used methods, for example,
It may be sterilized by methods such as air flow sterilization, high pressure sterilization, and heat treatment. In addition, if necessary, it is mixed with an excipient, a bulking agent, a binder, a thickener, an emulsifier, a coloring agent, a flavoring agent, a food additive, a seasoning, etc., and powders, granules, tablets, etc. depending on the application. It can be molded into a form, and can be mixed with various foods and drinks and used for eating and drinking.

GA manufactured by the method of the present invention
Brassicaceae plant containing a large amount of BA, its dry powder,
It is also possible to purify a pure GABA product using squeezed juice or squeezed powder as a raw material. Particularly, kale is useful as a raw material for these.

[0092]

EXAMPLES Example 1 Infrared treatment was performed using fresh leaves of kale, fresh leaves of cabbage and broccoli shredded to a size of about 5 mm. Infrared treatment is performed using a 400 W infrared radiating device, each object to be treated is sealed so that water does not evaporate, a temperature sensor is inserted, and the internal temperature is controlled to 40 ° C., Irradiated for 1 hour.

After the infrared treatment, GABA (GABA) was measured under the following conditions using an amino acid automatic analyzer. <Operating conditions of automatic amino acid analyzer> Model: JLC-500 / V (JEOL Ltd.) Column: LCR-6, 4 mm x 90 mm (JEOL Ltd.) Mobile phase: lithium citrate buffer (JEOL Ltd.) ) P-21 (pH 2.98, Li 0.105 mol / l) 0 → 16.3 min P-12 (pH 3.28, Li 0.26 mol / l) 16.3 → 36.1 min P-13 (pH 3.46, Li 0.80 mol / l) 36.1 → 56.0 min P-14 (pH 2.83, Li 1.54 mol / l) 56.0 → 63.4 min P-15 (pH 3.65, Li 1.54 mol / l) 63.4 → 80.0 min Reaction liquid: ninhydrin / hydridanthin reagent (Wako Pure Chemical Industries, Ltd. ) Temperature: Column 35 ℃ (0 → 16.3min), 64 ℃ (15.3 → 31.0min) 44 ℃ (31.0 → 44.4min), 72 ℃ (63.4 → 80.0min) Reaction tank 135 ℃ Flow rate: Mobile phase 0.50 ml / min Reaction solution 0.30 ml / min Measurement wavelength: 570 nm

The results are shown in Table 1.

[0095]

[Table 1]

The results in Table 1 show that the GABA content in cruciferous plants was increased by the heat retention treatment. The GABA content increased about 3.6 times in broccoli, about 5.9 times in cabbage, and about 6.2 times in kale.

Example 2 Fresh kale leaves were treated with infrared rays, warm air, and warm water. The infrared treatment was carried out in the same manner as in Example 1, the warm air treatment was carried out by applying hot air of 40 ° C. in an incubator under sealed conditions, and the hot water treatment was carried out by keeping warm water of 40 ° C. GABA was measured after each treatment. The results are shown in Table 2.

[0098]

[Table 2]

The results in Table 2 show that the GABA content in the kale leaves is increased by the heat treatment regardless of the treatment method. In particular, the infrared treatment increased about 6.7 times, and the warm air treatment and the hot water treatment each increased 3.2 times.
The GABA content was increased 2-fold and 3.8-fold.

(Example 3) Fresh kale leaves, shredded fresh kale leaves into 5 mm, and squeezed juice obtained by crushing fresh leaves and filtering them at 40 ° C for 1 hour as in Example 1. After that, the GABA content was measured. The results are shown in Table 3.

[0101]

[Table 3]

The results shown in Table 3 indicate that the GABA content is increased regardless of the form to be treated, that is, the GABA content is increased by enriching the fresh leaves, shredded fresh leaves and juice of fresh leaves with GABA. Is shown. GABA was increased 6 to 7 times or more as compared with that before the treatment.

Example 4 Since the infrared treatment was considered to be effective, the optimum temperature of the infrared treatment was examined. GABA was measured after the fresh kale leaves were treated at the temperatures shown in Table 4. The results are shown in Table 4.

[0104]

[Table 4]

The results in Table 4 show that the optimum temperature is around 40 ° C. At 25 ° C or lower and 55 ° C or higher, the GABA content tends to decrease.

(Example 5) Since infrared treatment at 40 ° C was considered to be effective, the optimum time for infrared treatment was examined. GABA was measured after treating the fresh kale leaves at 40 ° C. for the time shown in Table 5. The results are shown in Table 5.

[0107]

[Table 5]

The results shown in Table 5 show that the G
It can be seen that the ABA content tends to increase,
If it is treated for 30 minutes or more, it will increase about 3 times, and about 1 to 6
It shows that the maximum value is reached in time.

(Example 6) Next, using fresh kale leaves,
The increase in GABA content due to anaerobic treatment was examined.
For anaerobic treatment, fresh kale leaves are packed in a plastic bag, air is evacuated and filled with nitrogen gas, which is then stored in an incubator for 2 days.
The temperature was kept at 5 ° C. and the time was as shown in Table 6. 40
The treatment at ℃ was performed by irradiating infrared rays under anaerobic conditions.
The results are shown in Table 6.

[0110]

[Table 6]

The results shown in Table 6 are obtained by anaerobic treatment at 25 ° C.
It was found that the GABA content in the fresh leaves of kale was increased 3-fold or more, which shows that the GABA content is increased even in the anaerobic treatment. Further, it can be seen that the GABA content is increased about 7.4 times by performing the heat retention treatment (infrared treatment) at 40 ° C.

Example 7 Fresh kale leaves were heat-treated at 40 ° C. for 1 hour (infrared treatment), dried in a dryer at 60 ° C. so that the water content was 5% or less, and crushed with a blender. Thus, GABA-enriched kale dry powder was obtained. Fresh kale leaves that had not been heat-retained were treated in the same manner to obtain dried kale powder that had not been GABA-enriched. Table 7 shows the results of measuring the GABA content of each dry powder.

[0113]

[Table 7]

The results in Table 7 show that the GABA content of the dry GABA-enriched powder was increased about 8.7-fold.

Example 8 Fresh kale leaves were heat-treated at 40 ° C. for 1 hour (infrared treatment), crushed with a mixer to squeeze, and filtered to remove GABA-enriched kale squeezed. I got the juice. Fresh kale leaves that were not heat-insulated were treated in the same manner to obtain kale juice that had not been GABA-enriched. Table 8 shows the results of measuring the GABA content of each juice.

[0116]

[Table 8]

The results shown in Table 8 are obtained by the GABA enrichment treatment.
It shows that the GABA content in the squeezed juice increased 6.5-fold.

Example 9 Fresh kale leaves were heat-treated at 40 ° C. for 1 hour (infrared treatment), crushed with a mixer to squeeze, and filtered to remove GABA-enriched kale squeezed. After obtaining the juice, it was freeze-dried and further crushed with a blender to obtain a GABA-enriched kale squeezed dry powder.
Fresh kale leaves that have not been heat-insulated are treated in the same manner, and G
A kale juice dry powder not subjected to ABA enrichment treatment was obtained.
Table 9 shows the results of measuring the GABA content of each squeezed dry powder.

[0119]

[Table 9]

The results shown in Table 9 show that the GABA-enriched treated squeezed dry powder increased the GABA content by about 9.6 times.

(Example 10) Fresh young leaves of kale were picked, washed with water, drained and shredded into pieces of about 3 cm square. 100 g of the output was measured with a 2450 MHz, 500 W output device (microwave oven, RE-121 manufactured by Sharp) as shown in Table 1.
The microwave irradiation treatment was performed for the time described in 0.
Then, using a dryer (manufactured by Sanyo, MOV-112S), it was dried at 60 ° C. for 6 hours and pulverized with a blender to obtain a kale powder. The water content was 5% or less.

Comparative Example 1 100 g of shredded kale was mixed with 9
Immerse in 1 liter of hot water at 5 ℃, hold for 3 minutes, immediately 5 ℃
It was transferred to cold water, immersed for about 5 minutes, and cooled. After cooling, it was dehydrated by a centrifugal separator for about 45 seconds, dried in the same manner as in Example 10, and pulverized. However, it took 10 hours to dry.

Comparative Example 2 100 g of shredded kale was
The mixture was poured into 1 L of hot water at 95 ° C containing 7.5 g of sodium chloride and 7.5 g of sodium bicarbonate, dried in the same manner as in Comparative Example 1, and pulverized.

The results are shown in Table 10. In Table 10, "untreated" means that the shredded kale before the treatment was left as it was, or dried at 60 ° C. for 6 hours, pulverized with a blender, and pulverized. The numerical value is the dry weight conversion value,
The unit is mg / 100g. In addition, vitamins were measured by high performance liquid chromatography.

[0125]

[Table 10]

From the results shown in Table 10, GABA was retained in the Brassicaceae plants (kale) that had been subjected to microwave treatment, as compared with the conventional Brassica plants that had been subjected to blanching treatment with hot water or salt water containing sodium bicarbonate. further,
Vitamin B1 and vitamin C were also well retained.

Furthermore, the microwave-treated kale and the conventional kale were each put in an aluminum package and protected from light under the environment of 40 ° C. and a humidity of 75% for 2 months. The green color of the microwave-treated kale did not fade and retained a vivid green color, but the untreated green leaves of the kale faded from green and changed to a color close to brown.

Example 11 Picking fresh leaves of kale and 4
Infrared treatment was performed at 0 ° C. for 1 hour. The infrared treatment was performed by using an infrared irradiation device, sealed so that water did not evaporate, and controlled while controlling the internal temperature to 40 ° C. After that, blanching treatment is performed with hot water to obtain Example 7.
A powder was obtained by drying in the same manner as in. On the other hand, the kale leaves that had been cut into 3 cm squares were similarly subjected to infrared treatment for GABA enrichment treatment, then subjected to blanching treatment with hot water, squeezed to obtain juice, and dried to obtain powder. The GABA content of the kale leaf, the juice, the dried powder of the kale leaf, and the dried powder of the juice extracted after the GABA enrichment treatment and the branching treatment were measured.

As a comparison, without GABA enrichment treatment,
It was blanched with hot water, dried and pulverized.
The results are shown in Table 11.

[0130]

[Table 11]

This result shows that GABA enriched treatment-branching treatment left GABA which was almost completely eliminated in the past, and 10 mg / 100 g or more of GABA remained in the plant. . That is, in the comparative example, since GABA enrichment treatment was not performed, 1.5 mg / 100 g of kale leaves and 5.
Only 2 mg / 100 g of GABA remained, but in the example, 10.1 mg / 100 g of kale leaves,
GABA remained at 14.1 mg / 100 g by squeezing, 67 mg / 100 g by dry powder of kale leaves, and 85 mg / 100 g by squeezing powder.

Example 12 Fresh kale leaves were picked and subjected to infrared treatment in the same manner as in Example 11 to enrich GABA. Then, the microwave irradiation treatment and the drying treatment were performed in the same manner as in Example 10. For comparison, GABA
GABA content of kale which was subjected to blanching treatment with hot water without being enriched and dried and powdered was measured. The results are shown in Table 12. In the leaf column, only microwave treatment was carried out, and the numerical values are values for wet weight. The column of dry powder is a value obtained by drying after microwave treatment and is a value with respect to dry weight.

[0133]

[Table 12]

The results in Table 12 are obtained by the method of the present invention.
ABA is contained at an extremely high concentration of 1200 mg / 100 g. That is, it is shown that a dry powder of a cruciferous plant, which retains a green color, containing 240 times or more GABA as compared with the conventional method can be obtained.

From the above, according to the conventional method, although the green color is retained, GABA, vitamin B1 and vitamin C, which are the active ingredients in the kale, almost disappear, but the microwave-treated kale is GABA, vitamin B.
It can be seen that 1 and Vitamin C are maintained almost unchanged, thus demonstrating the usefulness of the cruciferous plant of the present invention.

[0136]

INDUSTRIAL APPLICABILITY The present invention provides a Brassicaceae plant having an increased GABA content, and the Brassicaceae plant having an increased GABA content is directly used for food. Further, by drying the cruciferous plant having an increased GABA content, a dry cruciferous plant powder containing a large amount of GABA is provided. Furthermore, by squeezing a Brassicaceae plant with an increased GABA content, GABA
A squeezed juice rich in sucrose is provided, and a dry squeezed powder rich in GABA is also provided. Accordingly, in addition to the conventional cruciferous plant juice and dry powder, there is provided a health food having a new added value of having a blood pressure lowering action.

Furthermore, in the production of cruciferous plants, dry powders of cruciferous plants, squeezing of cruciferous plants, dry powder of crushing of cruciferous plants, a microwave treatment is carried out to reduce the GABA content. In other words, a cruciferous plant, a cruciferous dry powder, a cruciferous juice, and a cruciferous juice dry powder having a green color are obtained.

The obtained plant of Brassicaceae, which has a high GABA content or retains a green color without lowering the GABA content, its dry powder, squeezed juice, or squeezed dry powder can be directly used for food and drink. Can be mixed with excipients, fillers, binders, thickeners, emulsifiers, colorants, flavors, food additives, seasonings, etc., and molded into powder, granules, tablets, etc., depending on the application. In addition, it can be blended with various foods and drinks and used for eating or drinking, or can be used as a drug raw material.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI A23L 2/52 A23L 2/00 F A61K 35/78 Q (58) Fields investigated (Int.Cl. ⁷ , DB name) A23L 1 / 30-1/305 A23L 1/212 A23L 2/00-2/52 A23B 7/00-7/06

Claims (17)

(57) [Claims] 1. A holding the green, and the γ- amino butyric acid A method of manufacturing a luer Vrana Plants to contain more than 10 mg / 100 g, with respect to (a) crucifers, 25
For anaerobic treatment at 50 ° C and heat retention treatment at 25-50 ° C
At least one of γ- aminobutyric acid enrichment process among 30
A step of performing the treatment for at least one minute to 24 hours ; and (b) subjecting the treated Brassicaceae plant to at least one of a blanching treatment and a microwave treatment. Wherein holding the green, and the γ- amino butyric acid A method of manufacturing a dry powdered luer Vrana Plants to contain more than 10 mg / 100 g, with respect to (a) crucifers, 25 Anaerobic treatment at 50 ° C and at 25-50 ° C
A step of performing at least one γ-aminobutyric acid enrichment treatment for 30 minutes to 24 hours in the heat retention treatment ; (b) a step of subjecting the treated cruciferous plant to at least one of a blanching treatment and a microwave treatment; And (c) a step of drying the cruciferous plant treated with (b). Wherein holding the green, and the γ- amino butyric acid A method of manufacturing a juice luer Vrana Plants to contain more than 10 mg / 100 g, with respect to (a) Brassica,
Anaerobic treatment at 25 to 50 ° C and heat retention at 25 to 50 ° C
Performing at least one of the treatments for enriching γ-aminobutyric acid for 30 minutes to 24 hours ; (b) pressing the treated Brassicaceae plant; and (c) squeezing juice obtained by the pressing. A method comprising the step of performing a microwave treatment. 4. hold the green, and the γ- amino butyric acid A method for producing a dry powder juice luer Vrana Plants to contain more than 10 mg / 100 g, with respect to (a) Brassica , Anaerobic treatment at 25 to 50 ° C and 25 to 50
A step of performing at least one γ-aminobutyric acid enrichment treatment for 30 minutes to 24 hours in a heat retention treatment at ℃ ; (b) a step of squeezing the treated Brassicaceae plant; (c) obtained by the squeezing A method comprising a step of subjecting the juice to a microwave treatment; and (d) a step of drying the juice treated in the step (c). 5. holds green, and the γ- amino butyric acid A method of manufacturing a juice luer Vrana Plants to contain more than 10 mg / 100 g, with respect to juice of (a) Brassica , Anaerobic treatment at 25-50 ° C and at 25-50 ° C
The step of subjecting at least one γ-aminobutyric acid enrichment treatment to the heat treatment for 30 minutes to 24 hours ; and (b) the step of subjecting the treated juice to a microwave treatment.
Method. 6. holds green, and the γ- amino butyric acid A method for producing a dry powder juice luer Vrana Plants to contain more than 10 mg / 100 g, juice of (a) Brassica Against anaerobic treatment at 25 to 50 ° C. and 25
A step of performing at least one γ-aminobutyric acid enrichment treatment of the heat retention treatment at -50 ° C for 30 minutes to 24 hours ; (b)
A method comprising a step of subjecting the treated juice to a microwave treatment; and (c) a step of drying the treated juice of (b). 7. holding the green, and, .gamma.-amino butyric acid A method of manufacturing a luer Vrana Plants to contain more than 10 mg / 100 g, 2450 MHz, 50 in cruciferous plants
A method for producing a cruciferous plant, comprising a step of performing a microwave treatment in which a microwave of 0 W is irradiated for 0.5 to 10 minutes . 8. hold the green, and the γ- amino butyric acid A method of manufacturing a juice luer Vrana Plants to contain more than 10 mg / 100 g, the juice of cruciferous plant 2450
MHz, 500W microwave for 0.5-10 minutes
A method for producing juice of a cruciferous plant, which comprises the step of performing a microwave treatment in which irradiation is performed for a period of time . 9. Anaerobic treatment at 25 to 50 ° C. and 25 to 50 ° C.
Γ-ami of at least one of the heat treatment at 50 ° C.
Nobutyric acid-enriched treatment is carried out for 30 minutes to 24 hours, and at least one of blanching treatment and microwave treatment is carried out , the green color is retained and 10 mg of γ-aminobutyric acid is obtained. / Brassica plants other than kale or squeezed juice containing 100 g or more. 10. Anaerobic treatment at 25 to 50 ° C. and 25
Γ-A of at least one of the heat insulation treatment at -50 ° C.
The minobutyric acid-enriched treatment is carried out for 30 minutes to 24 hours, and at least one of the blanking treatment and the microwave treatment is carried out , the green color is retained,
A dry powder of a cruciferous plant other than kale containing 10 mg / 100 g or more of γ-aminobutyric acid. 11. Vitamin B1 of 0.1 mg /
Dry powder of a cruciferous plant other than kale according to claim 10, containing 100 g or more and vitamin C of 100 mg / 100 g or more. 12. Anaerobic treatment at 25 to 50 ° C. and 25
Γ-A of at least one of the heat insulation treatment at -50 ° C.
The minobutyric acid-enriched treatment is carried out for 30 minutes to 24 hours, and at least one of the blanking treatment and the microwave treatment is carried out , the green color is retained,
A dry powder of juice of cruciferous plants other than kale containing 10 mg / 100 g or more of γ-aminobutyric acid. 13. Vitamin B1 of 0.1 mg /
A dry powder of juice of a cruciferous plant other than kale according to claim 12, containing 100 g or more and 100 mg / 100 g or more of vitamin C. 14. Anaerobic treatment and / or 25 to at least one plant selected from the group consisting of kale, cabbage, and broccoli or juice thereof.
A method for increasing the γ-aminobutyric acid content of the plants or their juices, which comprises the step of heat-retaining at 50 ° C. 15. Anaerobic treatment and / or 25 to at least one plant selected from the group consisting of kale, cabbage, and broccoli or juice thereof.
A method for producing kale, cabbage, broccoli or a juice thereof having an increased γ-aminobutyric acid content, which comprises a step of heat retention at 50 ° C. 16. A method for producing a dry powder of kale, cabbage, broccoli or juice thereof having an increased γ-aminobutyric acid content, which comprises (a) kale, cabbage, broccoli or juice thereof. Anaerobic treatment and
And / or γ-aminobutyric acid treatment for heat retention at 25 to 50 ° C
And (b) drying the treated kale, cabbage, broccoli or juice thereof. 17. A cruciferous plant other than kale according to claim 9 or its juice, a dry powder of a cruciferous plant other than kale according to claim 10 or 11, or a kale according to claim 12 or 13. A food, beverage, or pharmaceutical ingredient containing any of the dry powders of cruciferous juice other than.