JP2001151690A - Composition for oral cavity - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition which is useful for oral cavities and has an excellent halitosis-preventing effect and a good effect for preventing.improving the inflammation of gums. SOLUTION: This composition for oral cavities, characterized by containing dry Grifola frondosa powder and/or a Grifola frondosa extract.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an oral composition containing a dried powder of maitake or a maitake extract, and more particularly to an oral composition having an effect of preventing and improving gingival inflammation and an excellent effect of preventing bad breath. About things.

[0002]

2. Description of the Related Art Periodontal disease is two major diseases in the oral cavity along with dental caries, and accounts for about half of the causes of tooth loss. Plaque (plaque) bacteria and host defense mechanisms against the disease are involved in the onset of periodontal disease and progression of lesions. Among them, plaque, which is an inflammatory factor, is a major lesion factor, and the invasion is increased by an increase in the number of bacteria in the plaque and its pathogenicity. In addition, when the general condition deteriorates for some reason and the periodontal tissue repair ability and the defense function of the living body decrease, the equilibrium between the host and the bacteria is broken, and periodontal disease develops. At present, it is suggested that leukocytes (particularly multinuclear leukocytes) and monocyte macrophage system play an important role in host defense in periodontal tissue.

[0003] The functions of leukocytes in vivo include migration ability,
There are poor eating ability and bactericidal ability, and leukocytes migrating from the blood vessels of the gingival connective tissue to the gingival sulcus eat poorly foreign substances such as bacteria,
It plays an important role in biological defense mechanisms in periodontal tissue. However, at the same time in the inflamed area,
Leukocytes are also thought to be an important factor in tissue damage, and it has been reported that periodontal tissue is destroyed by leakage of cellular components and excessive production of reactive oxygen, especially in chronic inflammation . Therefore, it is useful to prevent periodontal tissue destruction by active oxygen by using a component that eliminates active oxygen.

On the other hand, halitosis includes physiological halitosis and pathological halitosis. Physiological halitosis refers to bad breath when waking up, bad breath on an empty stomach, bad breath when extremely nervous, bad breath during menstruation, and bad breath due to smoking or ingestion of a special odorant (such as garlic). Pathological halitosis is divided into those caused by systemic factors (bronchitis, diabetes, gastrointestinal diseases, etc.) and those caused locally in the oral cavity (periodontal diseases, caries, etc.). In particular, halitosis due to periodontal disease and dental caries accounts for 80 to 90% of cases, and is a problem that cannot be reduced in terms of dentistry as well as the person's troubles. Bad breath in the oral cavity (especially local inflammation) is caused by anaerobic bacteria decomposing food residues and gingival crevicular fluid on the gums. Its components are various such as sulfur compounds, lower fatty acids, alcohols and aldehydes, and among them, components related to the degree of bad breath are considered to be volatile sulfur compounds such as methyl mercaptan. From such a background, various active oxygen-scavenging components have been conventionally used in oral compositions, and a number of means for preventing and improving gingival inflammation and bad breath have been proposed, but all of them are still sufficiently satisfactory. There is nothing.

[0005]

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and has as its object to provide an excellent oral composition which prevents and improves gingival inflammation and has an effect of preventing bad breath. .

[0006]

Means for Solving the Problems The present inventors have conducted intensive studies in order to achieve the above object, and as a result, by including maitake dry powder and / or maitake extract in an oral composition, The present inventors have found that an oral composition having an excellent effect of preventing and improving gingival inflammation and giving an effect of preventing bad breath can be obtained, and completed the present invention. Therefore, the present invention relates to a composition for oral cavity, characterized by containing a dried maitake powder and / or a maitake extract. Specifically, the Maitake extract is an extract with at least one kind of water and / or a hydrophilic organic solvent selected from the group consisting of raw Maitake, dried Maitake, and dried Maitake.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail. Oral compositions referred to herein include toothpastes, powdered dentifrices, dentifrices such as liquid dentifrices, troches, tablets,
Examples include creams, ointments, patches, mouthwashes, mouthwashes, mouthwashes, chewing gums, and gargles. The raw maitake mushrooms are maitake (Grifola frondo
sa), White Maitake (Grifola albicans), Choreimaitake (Grifola umbellata), Tonmaimaitake (Grifola)
gigantea), and any of the fruiting bodies and mycelia of these maitakes can be used. In recent years, artificial cultivation of the fruit body of Maitake (Grifola frondosa) has become possible, and the use of the fruit body of Maitake is preferred from the viewpoint of securing stable raw materials.

As for the dried maitake, the sun, hot air drying,
Alternatively, any of freeze-dried products can be used. As the dried maitake, maitake dried powder obtained by pulverizing a dried product with a mill may be used. When the dried maitake is directly used for the oral composition, it is preferable to use it with a particle size of about 100 mesh or more. Maitake dry powder is commercially available, and a commercially available product can be used in the present invention.

As the extraction material, at least one selected from the group consisting of raw maitake, dried maitake, and dried maitake powder can be selected, and the raw maitake or dried maitake may be extracted to a certain degree to improve the extraction efficiency. Preferably, maitake dried powder can be used. Water, a hydrophilic organic solvent, and a mixture of water and a hydrophilic organic solvent can be used as an extraction solvent for obtaining an extract of Maitake. As the water, distilled water, purified water, ion-exchanged water,
Both tap water and natural water can be used. As the hydrophilic organic solvent, alcohols, for example, alcohols such as methanol and ethanol, and polyhydric alcohols such as glycerin, 1,3-butylene glycol, and propylene glycol can be used. When a mixture of water and a hydrophilic organic solvent is used as the extraction solvent, the ratio is generally preferably 1: 9 to 9: 1 in terms of mass ratio of water: hydrophilic organic solvent. For example, when water is used as the extraction solvent, the ratio of the extraction material and water is 2 mass or more per 1 mass of raw Maitake mushroom, preferably 5 to 5 mass.
When 10 mass of water is used and dried maitake or dried maitake powder is used, an appropriate amount of 4 mass or more, preferably 10 to 20 mass of water is used per 1 mass of the dried maitake.

The extraction method is not particularly limited, and may be carried out according to a conventional method, for example, under reflux or by immersion. For example, in the case of performing water extraction, from room temperature to 5 minutes to 3 hours under heating, and in the case of performing in a short time, under pressure, under conditions of 100 ° C. or higher, and under pressure using a pressure cooker, etc. Extraction is performed in the range of 5 minutes to 1 hour. The extract obtained as described above may be used as it is, but it can also be used as a dry extract extract powder by conventional drying means such as concentrated fluid extract or concentrated drying, spray drying, vacuum drying or freeze drying. . The extract contains a polysaccharide such as β-glucan or a conjugate of a polysaccharide and a protein, and a purified product can be obtained by precipitation, chromatography, gel filtration, or other means. The resulting purified product can also be used. Particularly when a purified product is used, an excellent effect can be expected. Dry extract powder and purified products can be obtained on the market, and the present invention can also use such commercially available products.

In the oral composition of the present invention, maitake dry powder or the maitake extract obtained as described above can be blended, and from the viewpoint of its effect, the total amount of the oral composition is 0.01%. % By mass or more, preferably 0.01
-20% by mass, more preferably 0.05-10% by mass. The oral composition of the present invention may be prepared according to a conventional method, such as toothpaste, powdered toothpaste, dentifrice such as liquid dentifrice, troche, tablet, cream, ointment, patch, mouthwash, mouthwash, mouthwash, chewing gum. Gargle, etc. The other components are not particularly limited, and components used in various oral compositions can be blended as long as the effects of the present invention are not impaired.

For example, when used for dentifrices, abrasives,
It can be produced by mixing components such as thickeners, sweeteners, preservatives, pigments, pH adjusters, various active ingredients, flavors and solvents. In addition, toothpaste, dibasic calcium phosphate, calcium carbonate, calcium hydrogen phosphate and other abrasives, carboxymethylcellulose sodium, hydroxyethylcellulose, carrageenan, binders such as polyvinyl alcohol, glycerin, sorbitol, propylene glycol, polyethylene glycol, Wetting agents such as 1,3-butylene glycol, foaming agents such as sodium lauryl sulfate and lauroyl sarcosine salt, essential oils such as peppermint, spearmint, xylitol, flavors such as menthol, sweeteners such as saccharin sodium, and parabens. An antiseptic is appropriately blended, and can be produced by using these components according to a conventional method. Powder toothpaste, liquid toothpaste, troche,
Tablets, creams, ointments, patches, mouthwashes, mouth fresheners, mouthwashes, chewing gums, gargles, and the like can also be produced according to standard methods by blending components according to the properties of the products. The oral composition of the present invention may further contain various drugs.

[0013]

Reference Examples, Test Examples, and Examples Hereinafter, the present invention will be described specifically with reference examples, test examples, and examples, but the present invention is not limited to the following examples.

[Reference Example] Manufacture of Maitake mushroom dry powder and Maitake extract extract (1) Maitake mushroom dry powder Approximately 60 ° C to about 80 ° C Hot air was sent to dry.
Initially, the temperature was increased stepwise from 60 ° C and finally dried at 80 ° C for approximately one day. Then, the dried Maitake was crushed with a mill to obtain a fine powder. (2) Maitake extract A 5 kg of raw Maitake was sliced to an appropriate size, immersed in a 20-liter hot water bath (90 ° C. or higher) for 1 hour to perform extraction, and then filtered to obtain a black-brown extract. Was. The extract was concentrated under reduced pressure to normal pressure to obtain a concentrated solution having a Brix value of 30%, and then spray-dried using a spray-drying apparatus to obtain a dry brown powder of Maitake extract having a brownish brown color.

(3) Maitake extract B 1 kg of dried powder of maitake fruit body was treated with 10 liters of ion-exchanged water at 120 ° C. for 30 minutes under pressure, and then filtered to obtain 6.2 liters of a black-brown extract. . The solution was concentrated under reduced pressure to 2 liters, and 2 liters of 95% ethanol was added at room temperature.
A brown substance floating in the liquid or adhering to the wall was formed. These substances were removed by scooping with a wire mesh to obtain a brown solution. The solution was freed of alcohol under reduced pressure, and further concentrated under reduced pressure to a Brix value of about 30% to obtain a dark brown thick liquid. The solution was spray-dried using a spray-drying apparatus to obtain a fine brown powder 185 having a sweet aroma unique to Maitake.
g was obtained.

[0015]

[Test Example 1] Antioxidation of maitake extract (elimination of active oxygen)
Effect (1) Superoxide radical scavenging action (SOD-like action) (Experimental material) As the maitake extract of the experimental material, powdered maitake extract B was used, and the sample was prepared at 100 mg / ml with ultrapure water. The solution was used. (Experimental method) Calcium ion-containing Hanks solution (purchased from Wako Pure Chemical Industries, Ltd.) in a 96-well microplate 116.4
of the sample solution prepared so that the final concentration of the sample is 10 to 0.625 mg / ml, and further 20 μl is added.
5 μl of μM Cypridina luciferin derivative (purchased from Tokyo Chemical Industry Co., Ltd.) and 8.6 μl of 1 mM hypoxanthine (purchased from Wako Pure Chemical Industries, Ltd.) were added. 37 ° C
And then add 50 μl of 1 mU / ml xanthine oxidase (purchased from Wako Pure Chemical Industries, Ltd.)
The total luminescence per minute was measured with a chemiluminescence detector.

(2) Singlet oxygen scavenging action (Experimental method) 135 μl of 133 mM acetate buffer (pH 4.5) was dispensed into a 96-well microplate, 20 μl of the same sample solution as in (1) was added, and a 20 μM Cypridina luciferin derivative was added. (Purchased from Tokyo Kasei Sales Co., Ltd.) 10 μl and 40
0 mM hydrogen peroxide (purchased from Wako Pure Chemical Industries, Ltd.) 1
0 μl was added. After leaving at 25 ° C for 1 minute,
M Sodium hypochlorite (purchased from Wako Pure Chemical Industries, Ltd.) (25 μl) was added, and the total luminescence per minute was measured with a chemiluminescence detector.

The antioxidant effect is obtained by calculating the total luminescence amount (A) when no sample is added and the total luminescence amount (B) when the sample is added. %). In this case, the higher the inhibition rate, the stronger the scavenging effect of active oxygen. The experience shows that the test sample concentration (IC ₅₀ ) showing a 50% inhibition rate is excellent when the concentration (IC ₅₀ ) is 2.5 mg / ml or less. It was determined to have an antioxidant effect. Inhibition rate (%) = [1- (B) / (A)] × 100 The test results are as follows.

[0018]

TABLE 1 Inhibition rate of each sample (%) and an IC ₅₀ value As is clear from the above results, the Maitake extract had a strong scavenging effect on superoxide radicals and singlet oxygen, and had an excellent antioxidant effect.

[0019]

Test Example 2 Deodorizing Effect on Methyl Mercaptan (Experimental Material) A sample solution was prepared using maitake extract B as an experimental material and adjusting the concentration to 100 mg / ml with ultrapure water.
This was added to the reaction system to a final concentration of 10, 5, 1 mg / ml. (Experimental method) To a test tube having a content of 20 ml, 1.5 ml of a 0.1 M phosphate buffer and 1 ml of an optionally prepared sample (only 1 ml of ultrapure water for control) were added to adjust the pH to 7.5.
0.5 ml of a 1 μg / ml solution of methyl mercaptan (purchased from Wako Pure Chemical Industries, Ltd.) was added, immediately stoppered with silicon, stirred with a touch mixer for 5 seconds, and kept in a 37 ° C. water bath. After 6 minutes, inject 5 ml of air with a gas tight syringe,
After stirring for 5 seconds, 5 ml of the headspace gas was extracted, and the peak area of methyl mercaptan was measured by gas chromatography (column temperature: 100 ° C., carrier gas: N ₂ , detector: FPD). The same sample was repeated twice, and the deodorization rate (%) was calculated and evaluated from the average value as follows. Deodorization rate (%) = {(CS) / C} × 100 C: Methyl mercaptan peak area of control S: Methyl mercaptan peak area at the time of sample addition The test results are as follows.

[0020]

[Table 2] Deodorization rate of methyl mercaptan for each sample (%) As is clear from the above results, the Maitake extract had a high methyl mercaptan deodorizing effect.

[0021]

[Test Example 3] Improvement effect of maitake dry powder or maitake extract-containing composition on gingival inflammation (experimental method) Three adult men without systemic disease affecting periodontal disease were given each dentifrice three times a day 10 days, put an appropriate amount (approximately 1 g) on a commercial toothbrush and use it.
ngival index (hereinafter referred to as GI). The observation sites were 6 4 of the upper and lower incisors, the first premolars, and the first molars.
Tooth surface (mesial, distal, buccal, lingual). In addition, each test period is separated by more than one week, to cause inflammation,
Mouth cleaning was stopped for 3 days before the start of the test. The order of using each dentifrice was randomized. The dentifrice used was prepared by mixing dry powder of Maitake or Maitake mushroom extract B as shown in Table 3 with the following common composition 1 (% by mass).

[Common composition 1] Calcium phosphate for dentifrice 50.0% Carrageenan 1.0% Glycerin 20.0% Sodium lauryl sulfate 1.0% Methylparaben 0.1% Saccharin sodium 0.1% Fragrance 1.0% Maitake dried Powder or Maitake extract B Purified water described in Table 3 Total remaining 100.0%

[0023]

Table 3 Formulation of Maitake dry powder or Maitake extract B of each dentifrice ：: blended ×: not blended In the evaluation, the reduction value of the GI after the test with respect to the GI before the test was taken as the improvement degree, and (improvement degree / GI before the test) × 100 was obtained as the improvement rate (%). Table 4 shows the results.

[0024]

[Table 4] Improvement rate by using each dentifrice As shown in Table 4, the oral composition containing the maitake dry powder and the maitake extract B had a higher gingival inflammation improving effect than the non-blended oral composition, and an excellent improvement on the gingival inflammation. It was found to have an effect.

[0025]

[Test Example 4] Deodorizing effect on bad breath (Experimental method) Three healthy adult men (panelers A to C) having normal dentition without severe disease in the oral cavity were taken as subjects and were rinsed with mouthwash. The amount of methyl mercaptan generated from the spontaneously discharged saliva before was used as a control. 20 ml of mouthwash was gargled for 30 seconds, and saliva was collected for 5 minutes immediately after gargling, and the amount of methyl mercaptan generated from the saliva was measured. The amount of methyl mercaptan generated was determined by adding 1 ml of saliva collected to 1 ml of 0.1 M phosphate buffer (pH 7.0) containing 20 mM L-methionine in a vial having a capacity of 30 ml.
Immediately stoppered and stirred. After incubating at 37 ° C. for 24 hours, 300 μl of headspace gas was injected into the gas chromatograph, and the peak height of the generated methyl mercaptan was measured. The mouthwash used was the following common composition 2
(Mass%), and dried maitake powder or maitake extract B was blended as shown in Table 5.

[Common composition 2] Ethanol 10.0% Glycerin 5.0% Polyoxyethylene hydrogenated castor oil 5.0% Menthol 0.7% Fragrance 0.1% Maitake mushroom dry powder or Maitake extract B described in Table 5 Purified water remaining 100.0%

[0027]

Table 5 Formulation of Maitake dry powder or Maitake extract B in each mouthwash ：: compounded ×: not compounded The bad breath prevention effect of the mouthwash was defined as the residual amount (%) of the amount of methylmercaptan generated in saliva after mouthwash, with the amount of methylmercaptan generated from saliva before mouthwash being 100. . The test results are as follows.

[0028]

[Table 6] Residual rate of methyl mercaptan by using each mouthwash (%) As shown in Table 6, the oral composition containing the maitake dry powder and the maitake extract B had a significantly lower residual ratio of methyl mercaptan than the non-formulated oral composition,
It turned out that it was excellent in the bad breath prevention effect.

[0029]

Example 1 Production of toothpaste A toothpaste was produced by a conventional method according to the following formulation (unit: mass%). Calcium hydrogen phosphate anhydrous 20.0 Calcium hydrogen phosphate dihydrate 20.0 Silicic anhydride 5.0 Carrageenan 0.7 Sodium carboxymethyl cellulose 0.3 Glycerin 20.0 70% sorbite solution 10.0 Paraoxybenzoate Methyl acid 0.1 Triclosan 0.1 Saccharin sodium 0.1 Sodium lauryl sulfate 1.5 Maitake extract B 0.1 Perfume 1.0 Purified water Remaining total 100.0%

[0030]

Example 2 Production of toothpaste A toothpaste was produced according to the following formulation (unit: mass%) by a conventional method. Silicic anhydride 20.0 Titanium dioxide 0.5 Carrageenan 1.0 Propylene glycol 3.0 70% sorbite solution 20.0 Glycerin 20.0 Methyl parahydroxybenzoate 0.1 Sodium monofluorophosphate 0.05 Cetylpyridinium chloride 0 1.1 Sodium lauryl sulfate 1.7 Sodium lauroyl sarcosine 0.3 Sodium copper chlorophyllin 0.05 Maitake extract A 0.1 Perfume 1.0 Purified water Remaining total 100.0%

[0031]

Example 3 Production of toothpaste A toothpaste was produced by a conventional method according to the following formulation (unit: mass%). Aluminum hydroxide 30.0 Silicic anhydride 5.0 Sodium chloride 10.0 Sodium carboxymethylcellulose (DS: 0.7) 0.1 Sodium carboxymethylcellulose (DS: 1.0) 0.9 Glycerin 20.0 Sodium lauryl sulfate 1.8 Lauroyl Sarcosine sodium 0.2 Isopropyl methylphenol 0.02 dl-α-tocopherol acetate 0.1 Polyoxyethylene hydrogenated castor oil 3.0 Propyl parahydroxybenzoate 0.05 Maitake extract B 0.1 Perfume 1.0 Purified water Remaining total 100.0%

[0032]

Example 4 A toothpaste was produced by a conventional method according to the following formulation (unit: mass%). Calcium pyrophosphate 32.0 Silicic anhydride 6.0 Sodium carboxymethylcellulose (DS: 0.7) 0.2 Sodium carboxymethylcellulose (DS: 1.0) 0.8 Glycerin 25.0 Glycyrrhizic acid 0.2 Cetylpyridinium chloride 0.05 Epsilon aminocapron Acid 0.1 Maitake extract B 0.1 Perfume 1.0 Purified water Remaining total 100.0%

[0033]

Example 5 A liquid dentifrice was produced by a conventional method according to the following formulation (unit: mass%). Silicic anhydride 10.0 Xanthan gum 1.0 Glycerin 10.0 70% sorbite solution 30.0 Xylitol 1.0 Ethyl parahydroxybenzoate 0.1 Butyl paraoxybenzoate 0.05 Sodium dihydrogen phosphate dihydrate 0. 3 Sodium hydroxide 0.05 Maitake extract A 0.1 Perfume 0.5 Purified water Remaining total 100.0%

[0034]

Example 6 A mouthwash was produced according to the following formulation (unit: mass%) by a conventional method. Denatured Alcohol No. 56 5.0 Polyoxyethylene hydrogenated castor oil 0.5 Glycerin 5.0 Xylitol 25.0 Cetylpyridinium chloride 0.05 Dipotassium glycyrrhizinate 0.05 Ethyl paraoxybenzoate 0.05 Propyl paraoxybenzoate 0.05 Citric acid 0.03 Sodium citrate 0.12 Yellow No. 4 0.0002 Green No. 3 0.0002 Maitake extract B 0.1 Purified water Total remaining 100.0%

[0035]

The oral composition of the present invention has an excellent effect of preventing and improving gingival inflammation and an excellent effect of preventing bad breath by blending a dried powder of Maitake or a Maitake extract. It is.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 9/20 A61K 9/20 9/68 9/68 9/70 9/70 A61P 1/02 A61P 1 / 02 29/00 29/00 (72) Inventor Keiichiro Kondo 3-20-7 Kobuchi, Sagamihara-shi, Kanagawa Prefecture (72) Inventor Yasuo Ohira 2610-4, Oyokawa, Okamachi, Minamiuonuma-gun, Niigata Prefecture (72) Inventor Masataka Watanabe 729-2, Tashita-cho, Utsunomiya-shi, Tochigi (72) Inventor Masahide Takeyama 33-2, Omiya-shi, Saitama F-term (reference) 4C076 AA06 AA11 AA29 AA36 AA49 AA69 AA72 BB22 BB23 CC04 CC09 CC31 DD26 DD29 DD35 DD38 DD43 DD55 DD61 EE32 EE58 4C083 AA111 AA112 AB172 AB222 AB242 AB282 AB292 AB332 AC102 AC122 AC132 AC302 AC432 AC472 AC482 AC622 AC662 AC782 AC792 AC812 AC852 AC862 AC932 AD272 AD352 AD532 AD662 CC41 DD12 DD15 DD17 DD22 DD23 DD31 EE32 EE33 EE34 4C088 AA04 AC01 BA07 BA09 BA10 CA01 CA03 MA17 MA27 MA28 MA32 MA35 MA47 MA57 ZA67 ZB11

Claims (5)

[Claims] 1. An oral composition comprising maitake dry powder and / or maitake extract. 2. The maitake extract is an extract of at least one water and / or hydrophilic organic solvent selected from the group consisting of raw maitake, dried maitake and dried maitake powder. Oral composition as described 3. The oral composition according to claim 1, wherein the composition contains 0.01% by mass or more of Maitake mushroom dry powder and / or Maitake mushroom extract. 4. The oral composition according to claim 1, wherein the composition contains 0.01% to 20% by mass of the dried maitake powder and / or the maitake extract. 5. An oral composition comprising a dentifrice, a dentifrice, a liquid dentifrice, a troche, a tablet, a cream, an ointment, a patch, a mouthwash, a mouthwash,
The oral composition according to any one of claims 1 to 4, which is a mouthwash, chewing gum, or gargle.