JP2001112466A - Cosmetic containing new lactobacillus fermentation metabolite - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic and a bathing agent which contain a new and safe moisturizing agent and/or a new and safe immunodepression-preventing agent which has skin-improving effects with the passage of time, are effective for preventing and treating skin roughness and dry skins, and can further give gloss and tension to the skins. SOLUTION: This moisturizing agent for cosmetics or the immunodepression- preventing agent contains the fermentation metabolite of a new lactobacillus, Lactobacillus delbueckii subsp. bulgaricus TY569 (FERM P-17488) as an active ingredient. In addition, a cosmetic or a bathing agent containing the same.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a humectant for cosmetics, a preventive agent for reducing immunological deficiency for cosmetics and an application thereof to cosmetics and baths containing a fermented metabolite of a specific lactic acid bacterium as an active ingredient. is there.

[0002]

2. Description of the Related Art It is well known that when the water content in the stratum corneum is reduced to 10% or less due to the cold winter season or drying due to sunburn, the skin becomes rough, rough and rough. In addition, aging tends to cause rough skin due to insufficient secretion of sebum on the skin surface or insufficient water in the skin. Rough skin is characterized by drying, exfoliation, texture disturbance, and the like of the stratum corneum of the skin.

[0003] Rough skin due to reduced moisture retention is common in dry skin, but it is natural to consider chronic rough skin as a precursor to wrinkles and fine wrinkles. In addition, when various changes occurring on the epidermis become chronic, the changes often affect the entire skin, sometimes leading to abnormal skin inflammation and changes in the immune reaction system and endocrine system.

[0004] Skin care for such rough skin includes various effects on the skin, such as protecting the skin from excessive ultraviolet rays and dryness, and moisturizing creams on the skin, whose moisturizing function deteriorates with aging. Supplement active ingredients, promote blood circulation with massage and cooling / heating stimuli, improve the supply of nutrients to the skin, etc., to maintain the function of maintaining the homeostasis of the skin. Attempts have been made to use a foundation together.

[0005]

However, conventional cosmetic compositions have not been satisfactory in terms of feel, sustainability of moisturizing effect, safety, and physicochemical stability. In particular, the moisturizing effect and the ultraviolet ray preventing effect of the conventional components are temporary, and it is hard to say that the composition for external use is sufficient for maintaining and regenerating healthy skin or hair, such as prevention or improvement of rough skin and split ends. there were.

[0006]

In view of these circumstances, the present inventors have developed various natural products having a beautiful skin effect against injuries such as ultraviolet rays, and as a result, the fermentation metabolites of the novel lactic acid bacteria have been moisturized and improved. It was confirmed that it had an effect of suppressing immunity decline. Therefore, the present invention can be effectively used as a moisturizing agent and / or a preventive agent for lowering immunity, and is a novel and useful agent for preventing and treating the drying and roughening of the skin or scalp and preventing immunity lowering induced by ultraviolet rays. Provide safe cosmetics and bath preparations.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The “fermentation metabolite of a novel lactic acid bacterium (hereinafter referred to as a lactic acid bacteria fermentation metabolite)” used in the present invention is:
Lactic acid bacteria Lactobacillus delbrueckii bulgaricus TY569 (Lactobacillus delbueckii subsp.bulgari)
FERM P-17488), and the lactic acid bacteria have the following mycological properties. Bacterial morphology when cultured in MRS liquid medium at an initial pH of 5.6 at 37 ° C for 18 hours (1) Bacterial bacillus (2) Gram stain positive (3) Motility None (4) Spores None (5) Catalase None (6) Facultative anaerobic (7) Growth temperature range 30-45 ° C (8) Growth pH range 5.0-7.5 (9) Lactic acid fermentation homo-type (10) Lactic acid illuminating D (-) (11) Sugar Glucose, fructose, mannose and lactose are positive. In the present invention, those obtained by separating and removing an individual from the culture in the title, or those extracted or prepared with a solvent can be used.

Examples of the extraction solvent include water, alcohols (eg, lower alcohols such as methanol, anhydrous ethanol and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, and diethyl alcohol. Esters such as ether, dioxane, acetonitrile and ethyl acetate, and organic solvents such as xylene, benzene and chloroform can be used alone or in any combination of two or more. A combination of objects can also be used.

[0009] The fermented metabolites of lactic acid bacteria are applied humectants,
Drying, concentration, dilution or the like may be arbitrarily adjusted depending on the dosage form and form of the immunological reduction preventive agent, cosmetic or bath agent.

Although the extraction method is not particularly limited, it is usually sufficient if it is within the range of the boiling point of the solvent at normal temperature to normal pressure, and after extraction, filtration or ion exchange resin is used to perform adsorption, decolorization, Purification may be performed to obtain a solution, paste, gel, or powder. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected.
An activated carbon column or the like may be used as a purification treatment means for decolorization or the like, and any ordinary means generally applied to an extracted substance may be selected.

The amount of the humectant or immunosuppressant according to the present invention to be added to cosmetics or baths is usually 0.001% by weight or more (hereinafter expressed as%), preferably 0.01% by weight, as a dried product of the metabolite of fermented lactic acid bacteria. ~ 10% is good. In addition, 0.001
If the content is less than 10%, it is difficult to obtain a sufficient effect, and if the content is more than 10%, the effect of proportion-proportional reflection does not appear, which is uneconomical.

The humectant, the immunosuppressant, the cosmetic and the bath agent of the present invention may further comprise, if necessary,
As long as the effects of the present invention are not impaired, components and additives used in pharmaceuticals, quasi-drugs, cosmetics and the like can be arbitrarily selected and used in combination.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed Oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.) )Such.

(2) waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oils Liquid paraffin, vaseline, paraffin, ozokelide, ceresin, microcrystalline wax and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid Synthetic fatty acids such as, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) alcohols ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, 2-hexyldecanol,
Synthetic alcohols such as isostearyl alcohol and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, sugar or water-soluble polymer compound Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose or its ester, trehalose or its derivative, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, alkylene such as ethylene oxide (C2-C4)
Oxide-added hydroxyalkyl (C2-C
4) chitin or chitosan, low molecular weight chitin or chitosan,
Chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or a salt thereof, hyaluronic acid or a salt thereof, chondroitin sulfate or a salt thereof, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose , Hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide or polypropylene oxide or a crosslinked polymer thereof, carboxyvinyl polymer, Polyethyleneimine and the like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester nonionic surfactants, ester nonionic surfactants, block polymer nonionic surfactants, nitrogen-containing nonionic surfactants, and other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactants, surfactants containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: vitamin C acid or a derivative thereof, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3),
Dihydrotaxelol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K
2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U Such.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a conventional manner according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decoloration, etc. You can choose to serve.

The solvent used for the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later. However, usually, water or a water-soluble organic solvent (for example, ethanol) is used. ,Propylene glycol,
It is preferable to use one or a mixture of two or more selected from among 1,3-butylene glycol). However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations, cosmetics and bath preparations, including protection of the skin and hair, moisturizing, improving feel and texture, imparting flexibility, relieving irritation, relieving stress by fragrance, Cell activation (prevention of cell aging), suppression of inflammation, improvement of skin and hair quality, prevention and improvement of rough skin, hair growth, hair growth, prevention of hair loss, gloss imparting, cleaning effect, relief of fatigue, promotion of blood flow, warm bath In addition to cosmetic effects such as effects, effects such as fragrance, deodorization, thickening, preservation, and buffering can be expected.

Specific plants (crude drugs) as raw materials include:
For example, almonds (Hentomo), eye (Aiba), Blue Kazura
(Seifu Fuji), Aoki (Aoki), Aokiri or Kenasia Ogiri
(Otogi), Aotsumuraji (Kibo), Acacia, Akashoma (Akashoma), Akasuguri "fruit", Akagrape, Akaminoki (Logwood), Akamegawiwa (Akabu Kashiwa), Akane
(Akane grass root), Acacia elegans or Jiou (Jihuang), Agi (Awei), Aki larch, Akinire (Royu skin), Aguahe (Omiten palm), Akebi (Kidori), Asa (Ajihito), Malva Asagao or Asagao (Towushi) ), Hydrangea (hydrangea), ashitaba (morning leaf), adzuki (red azuki bean), acerola, asenyaku (asenyaku), acira (shokuyoukanna), anise, abemaki `` fruit '', avocado, ama, amacha (sweet tea), Amachaworu, Amadokoro (Jade bamboo), Amana (Hikarigi), Amaranthus (Amaranthus), Amaranthus (Hymoptera), Senninkoku, Sugimorigeitou, Hosoaoigaito, Aogetoudo, Haribiyu, Amaranthus hippochondriacus, Amigasayuri or Birnamo Alcanna), Argarrobo (cabbage), Altea, Arnica,
Alpinia or red sea bream (Sou-zuku), aloe (lux),
Aloe vera, angelica, apricot or hon apricot (almond jelly), ansokkou (benzoic), Ikakozolina (bile head), epimedium or potato valerian (inyokaku), rush (light heart grass), knotweed (tiger stick root), yew (first place), Strawberry, fig (fructose `` fruit, leaf ''), strawberry (first time), strawberry (winter aoiko), ichiyakuso (one medicinal herb), ginkgo (ginko `` seed, leaf ''), Itohimehagi (enshi), carob, carob bean ( (Telelexi), Inubiyu (Honagainubiyu), Rice `` seed, seed coat '', Inondo `` seed '', Ibuki-kusou-so, nettle, ylang-ylang, iwa-tobacco (iwa-hi-chi-chi), iwahiba or iwa-matsu (makikashiwa), oolong tea, fennel-wheat, wheat (Chibusanoki), Ukiyagara (Mitsuryo), Uguisukagura `` fruit '', Himeuikyo, turmeric (Utsukin), duckweed (buoying), Usubasaishi Or Keirin Herba Asari or Scots. Recently (Hosokarashi), malva sylvestris, marshmallow, Prunella vulgaris (summer hay), Udo or Angelica (羌活, Udo, Tang Udo), Unyadegato (Uncaria, cat's claw), Uva tea, plum
(Umebashi `` seed, pulp ''), Vulgaris ogre, Uwaurushi (Kusaco peach), Unshu mandarin (Chin skin), Estragon, Ezocogi (Ezo Goka), Echinashi (Hosobamurasakibarengiku), Enishida, Enokitake (Enotake mushroom), Ebisugusa or Cassia tora (Keiko), Elcampre, Elderberry `` fruit '', Elemi, Eryngii or Pre-Lotus Erinji, Enju (Gossip, Sophoraceae rice), Ougi or Kibana-ohagi (Ohgi), Ouren (Yoren), Ookarasuuri
(Karokon), giant grove (civil engineering incense), giant clam (self-defense), giant psyllium (forehead child, forehead grass), toucan hazel (haruko), Obanana okera or okera (white jitsu), Obananasasurube (banaba), Obanayashabushi "fruit", Hawthorn or hawthorn (Yamakoko), Ousei (Naruko lily, Kagikurumabanarukoyuri), Oohisasanoyuki or Sasanoyuki, Barley (barley), Greater grass (Grass grass),
Oka, Okazeri (snake bed), Oguruma (turnover), Okra `` fruit '', Panax ginseng or carrot (carrot), Hypericum or Kogomebai perforated or Periwinkle (Brother's cut grass), Odorikosou (intermittent), Onamimomi (blue ears), Onigurumi, Onidokoro or Tokoro or Nagadokoro (Hikai), Oninoyagara (Tianma), Oniyuri or Sasasayuri or Hakatayuri (Yuri), Ononis, Halibut (Hibaha Yu), Ominaeshi (Loose), Oyuko, Dutch mustard (Crasson), Dutch jelly , Dutch honeybee, olives "fruits, seeds, leaves", oregano, orange "fruits, pericarp", calyxio (ripe yellow), cacao "fruits, pericarp,
Seeds '', oysters (persimmon `` leaves ''), kagi kazura (hootshoo), oysters or kantosou (rensensou), cassia, casino
(Kozoumi `` fruits ''), gajutsu (shajutsu), kasiwa (shaki), cascarilla, cascara sagrada, gypsophila, kanikusa (kinsato), kaniwa, kanokoso (valerian grass root), birch or shidare kamba (shirakaba) , Pumpkin, capoknoki "seed", kahoku sansho (shopper), tama (bamboo yellow), chamomile or roman chamomile, chamiyadee (tsukusa), camu cam
(Duck), Karaku salmon, Calla uri or Sinara cucumber (Gourd melon), Callas bishaku (Midsummer), Raspberry, Guarana `` seed '', Karahoo (Popular), Karaya, Karin (Gourd), Garcinia, Kawamidori, Kawara psycho (Koryosai) ,
Japanese white grass), Kawazusha, Kawaratake, Kawaranadeshiko (stone bamboo) or Ezokawaranadeshiko (Qumugi, Quumiko), Kawaran Ginseng (Aokko), Kawamuragi (Inchinko), Kansui (Kansai), Licorice (Lucifera), Kantarasa, Candelilla, canto, canna, raspberry (ezo strawberry, dutch strawberry, shrimp strawberry, pear strawberry, maple strawberry,
(European strawberry), Kiwi `` fruit, leaf '', Kikarasuuri (Ururone), Kikyo (Kikyo, Kikyo root), Chrysanthemum (Chrysanthemum flower), Simakangiku, Daffodilium), Catalpa (Azumino), Rigid japonica (Yotei) Pheasant (Kikomi), Kizuta, Kidachi aloe, Kidachimina, Kina, Kinoki (Shincona, Akakinoki), Quinoa (Quinoa), Kihada (Huangbashi),
Gymnema sylvesta, cabbage, cabbage `` immature fruit '', cucumber, gyoryu (Nishikawayanagi, Ichiyanagi), Kiraja saponaria, Kiraya, Kiranso (gold pea grass), kumquat `` fruit '', betel, kinmizukiki (senzaku grass), guava "Fruit", Guayule, Quercus Infectria
(Gallery), Kukui Nuts, Kukui (Gugi, Kikougi, Kikou Leaves, Skeletal Bark), Kusagi Kazura (Tenmen Winter), Kuzu (Kuzukane),
Camphor tree, gooseberry `` fruit '', ginseng, yellow gardenia, gardenia, kunugi, kupuasu,
Kumazasa, Kumamatsuura (horse whip grass), Clara (bitter butter), cranberry "fruit", chestnut "seed, fruit, astringent skin", curculigo latifolia "fruit", grapefruit "fruit /
`` Leaves '', buckthorn, black squirrel (needs rescue), black blossoms, or crab (extended life grass), cloves (cloves, cloves), gumbinazuna (sekimei, sekimeishi),
Kaigai (Thorndust, Ikutakeho), Celosia (Rooster, Chicken),
Bay laurel, kenashi sartorii rose (Tobukuryo, Yamakiko), gentian, gennoshoko (Old treasured),
Kemponashi (Kigushi), Koukisekikoku, Kishumitsukan
(Koji, Tachibana, Olivenimikan, Fukuromikan,
Sagami Koji, Ponkan, Santara (Tachibana skin)), Koshinbara (Monthly flower), Kouzi mint, Kozo `` fruit '', Kocha (tea), Kouhone (river bone), Kouhon (straw, Kara straw), Koryan, Koryokyo ( Kojiro), cilantro (fruit), coconut (kohuangren), koganebana (yellow gon), lingonberry (koshibachi), coconut palm (fruit), goshyu (goshuyu), goshoichigo (covered bonsai), pepper (pepper), kopaiba balsam , Coffee `` seed, leaves '', kobushi or magnolia (spicy), burdock (burdock, burdock), kobotdur, sesame (sesame), sesame seeds (ginseng), goji (sesame), scallop or binankazura or Matsubusa, wheat (wheat) ),
Rice or rice bran “red bran, white bran”, rice oil, cola acuminata “seed”, cola vera “seed”, fenugreek “fruit”,
Colombo, Konzulango, Kombu, Konjac, Comfrey (Shinbari grass), Sisal (Sisal Asa), Sakisima button or Sinata button or Sinensensou (Eisensen), Sakura (Oshimashima cherry, Yamazakura, Oyamazakura, Edhigashi, Mamezakura, Miyayamazakura, Someiyoshino, Takanezakura, Kasumizakura, Chojizakura, Kohigan, Satozakura, Kanzakura `` leaves, flowers, fruits, bark (cherry bark) ''), cherries, pomegranate, sasa, sasanqua, sagemodaka (sawashari), sweet potato, sugarcane,
Sugar beet, Sun jujube (acid jujube), saffron
(Bamboo safflower, West safflower), Pomelo `` fruit '', Soapwort, Salmonberry `` fruit '', Sarashina-sho (Masahima), Salvia (Sage), Sawagikyo (Yamakona), Sawagurumi (Yamagomo), Sangre degrad (Croton ), Panax ginseng, Panax ginseng (Sax carrot), Sanshuyu (sanshuyu), Sansho (Sansho), Sandzukon (Yamazone), Shea (Karite), Cyano (fruit), Shiitake (Shitake mushroom), Zion ( (Shien), Dictalis, Shikunshi (Kimiko), Perilla or Aodiso or Chirimenzo or Katamamenjiso (Shiyo-leaf, Shiso), Rosewood, Shinanoki, Shinanohonoki, Chinese Forsythia (Forsythia), Shimeji (Hinshimeji, Shakashimeji, Hatakeshimeji, Oshureshimaji) , Honjimeshi, Shimotamogitake), Spiraea, Potato, Peony (Shakuyaku), Shajin (Shasan), Jasmine (Ma Rika),
Jano-beard (Bakumon winter), Gypsophila paniculata, Sku-shamitsu (sand sand, sand compaction), Juzudama, Palm `` fruit '', ginger (ginger), jadean hydrangea (Joyama), shobu
(Irises, iris roots), shojiku `` fruits '', shirakashi `` seed '', white walnuts `` seed '', white adzuki bean (chicken bone), white iris (small iris), white napatsuta `` flower '', white nanten (minamitenmi), shinkonasakushi Rubra, Jingkoge (Mizuka, Mizuka, Shencho), Cinnamon, Cinnammom Cassia (Kihi), Watermelon (watermelon),
Honeysuckle (gold and silver flowers, Shinobi winter), sorrel (acid pattern), swim berry `` fruit '', stevia, strawberry `` fruit '', suzu psycho (Xu Chang Lord), horsetail (question thorn), suberhide (horse dent,
Horse-tooth Kenko), Plum `` fruit '', Pinus densiflora `` cones '', larch larch, eucalypt, corn walnut, eucalypt, dandelion, horse chestnut (malonie), eucalypt `` fruit '', eucalypt, (Elder), Aesculus serrata (Juniper, Juniper), Achillea millefolium (Mirfoils), Alaska roses, Alaska aurora,
Mistletoe, Common mint or Common mint, Horseradish, Sekisho (stone iris), Red pepper
(Ishi-masu), Cedron (Bowshu-boku), Gentle mallow, Hirohasenega, Senega, Seri, Celery, Senkyuu (Kawakyuu), Senshinren (Shinshinren), Sendan, Senburi (drug), Senna `` Fruits, leaves '', Senninsou (Large) Tate), souka (grass), buckwheat "seed", soybean potato, rhubarb (daisou), radish, soybean, daidai (orange peel, kikomi), takasaburo (dry lotus), takatodai (daiseki), takawarabi ),
Dark sweet cherry "fruit", hollyhock, hollyhock (thyme, morika), hollyhock (hikari), hollyhock, tamarind "seed", onion, tamashiba
(Spicy), Tara tree `` fruits, leaves, root bark '', tarwi, tanjin (dansin), dandelion (Kabukohide) or white dandelion or moko dandelion, dammar, cherry `` fruit '', tigaya `` fruit, root, bud '', chiksetsu Carrots (bamboo ginseng), chicory, chancapiedra (fat bean ginseng), daffodil (daisies), daffodil (buoy), artemisia (aussin), choreimaitake (horingu), chorogi,
Tsukimiso, Tsukuritake (mushroom), Camellia, Camellia, Tsumekusa (lacquer lacustrine), Tsumukusa (duckling grass), Tsuruazuki (red azuki bean), Tsurukudami (Hanshukara), Tsuruna (Banju),
Carrot (four-leaf ginseng), camellia, deiko, teuchigurumi, duberry `` fruit '', tengusa, tencha (tencha),
Tendai-yaku (crowd drug), touga (winter gourd), touokazuzura, pepper (bansho), touki (toki), tokinsenka
(Marigold), cornflower (river cut), corn or corn hair (nanban hair), horned rice mochi (femako),
Gentian or Korean gentian (dragon gall), horsetail
(Pirate), dokudami (jujutsu), radish (root), toshishi or beetle or pear, and eucommia (tochu `` bark,
`` Leaves, roots ''), ash (qin peel), tomato, tragacanth, triacanthos `` seed '', tormentilla, dronoki, troloioi, Niseria berry `` fruit '', potato or yam (herbal medicine), nagikadada (butcher bloom), naginata koju, Nazuna, oilseed rape, oilseed bean or sword bean (sword bean), jujube, jujube (daijutsu), nanawaibara
(Kanekurako), Navena (intermittent), Nameko, Narco lily (Yoshimi),
Peanut (peanut), Nanten (Minami Tenmi), Namban Karamushi (ramie), Nigaki (bitter tree), Japanese Artemisia (bitter), Nikuzuku, Kay or Nikkei or Ceylon Nikkei or Yabnikkei (cinnamon bark) or Keishi (Keishi), Chinese chive (Niriko), elderberry (bone-bearing tree `` fruit, flower, stem, leaf ''), garlic (garlic), nurde (five times), leek, nemoki or neb or nebi or nemuriki or digokbana (health), thistle (Oji) , Noibara (Fruit), Yarrow, Soybean (Maeko), Nobara, Nomomo, Palm, Pineapple "fruit", Hibiscus
(Bussouge, FULIN BUSOUGE, Roselle), Hi-Gindo, Hakamauraboshi (bone crushing), Hakusen (ringworm skin),
Huckleberry `` fruit '', chickweed (reproducible), hazel (haruko), hashidokoro (roth root), basil, lotus (lotus, lotus, lotus root), parsley (Netherland jelly), hamkamugi, batata, hachiku or matake (bamboo-boiled) , Patchouli, mint (light load, light load leaf), adlay (yokuinin), hanasuge (chimbo), banana, hanaminta, hanabishi (siturishi, shishitsuri), vanilla bins, papaya, japonicus (rat malt),
Pahalobobo, hub `` whole grass, stem, leaf '', paprika, hamago or honeysuckle (vine thorn), hamasuge (kosuke), hamabishi (shikoriko), hamanas (maikai flower), hamabafu (beach storm), hamamelis, rose (Rose), agaric (aragaric, agaricus agaricus, usukimori no kasa), haran, pariurus (Prunus avium), parietalia,
Balsamina (Tsurureishi, Nigauri), Harunile (Yellow, Yuhakuhide, Yuba), Harindou, Handaikai (Matsudaikai), Breadfruit, Hiougi (sun dried), Lycopodium carrot
(Party Party), Pu'er Tea (Pu-cha), Pecan Nut, Amaryllidaceae (Shirubi, Toranshasha), Hishi (Ryomi), Pistachio, Beet, Hitotsuba (Stone Reed), Hitotsubeniishida, Hinata no Kozuchi (Cow Knee) ), Cypress, Hiba, Castor, Sunflower, Bell Pepper, Himeuzu (Tenoi), Himegama (Kaba), Himematsutake
(Kawariharatake, Hiromatsutake), Pimenta `` fruit '',
Juniper, juniper, hyouyu `` fruit '', below-flower, hiroha okinagusa (bakuin), loquat `` fruit, leaf,
Stalks '', betel wax (large clothing skin, areca locust), sugar beetle (southern rattan), butterflies, coltsfoot (populations of winter flowers, coppice winter leaves), fujibakama (orchids), fujimame beans (flat beans), grapes `` fruits, peels, seeds, leaves '' , Beech, Fuyumushinatsukutake (Cordyceps), Brazilian liquorice, Brazilian carrot, Blackcurrant "fruit", Blackberry, Plum "fruit", Flucellaria, Blueberry (Prunus americana), Prune, Blonde psyllium, Bundou (green bean) , Hazelnut, loofah, safflower (safflower), henneken, belladonna, berry `` fruit '', Peruvian berg, benceisou or duckweed (Keiten), boysenberry `` fruit '', broom beetle or elderweed or endow or hakigi kokia (ground silk) , Balsam (Hosen, acute offspring, osteophyte), spinach, boufu (windbreak), spinach, ho Suzuki (Toro root), Magnolia obovata (KazuAtsushiBoku, Park), blur (quince), Hosobanaokera (Aoiokera), e buckwheat Roh Kirin Saw (Keiten thirty-seven), Hosobabarengiku, linden (菩醍 trees), button ( "flower,
Leaf, stem, bark "), buttonpi (peony bark), hop, jojoba, bordeaux, white berry" fruit ", hon-oniku
(Nikuju Ryo, Daigei), Hongsek-koku (iron bark stone, sparrow mountain stone mascara), mysurutennansha (tennan star), maitake (maitake), ephedra (mao), maca, macadamia nut, macri
(Sea grass), Magwa (mulberry white bark `` bark, leaves ''), magnolia
Splengeri, Mashua (Tamanousenharen, Kyukonkinrenka), Matatabi (Kiten Tate), Matsukasa, Pine `` leaves, bark, roots '', Matsuhod (Bukuryo), Matico (Cordon Sillo), Maryoram (Hanahakka), Malvanozadine (Bitter ), Mulberry "fruit", quince, mango,
Mangosteen, Mansagna (Andesca beetle), Mansuru walnut, Mandarin `` Fruit '', Mannentake (Lingzhi), Pheasant (Kikomi `` Fruit ''), Mishima saiko (Saiko), Mizuobako or Mizuasagao (Ryu tongoshi), Mizokoshi Lotus), misohagi (senkakuna), beet willow or elder willow
(Henshin), honeysuckle, honeysuckle, green peppermint, mimosa,
Ginger, miracle fruit `` fruit '', myrrh, mirobaran, wheat waragiku, mugwort (tree geun), mukunoki, mukuroji (extended life skin), munya, murasaki (purple root), murasakisikibu or omurasakishikibu (purple bead), murasaki maize, murasaki natsufuji Kunming chicken blood wisteria), swordfish (mother grass), scallop, melaleuca, melissa, melillot,
Melon "fruit", sagebrush, moso bamboo, moje
(Keikoboku), Mokko (Kiko), Japanese Maple,
Peach (peach `` leaves, seeds, flowers, fruits ''), sprouts, morello cherries `` fruits '', moroheiya (burlap), yakawamurasakiimo, yakuchi (masuti), cornflower (cornflower), cornflower mint, yacon, yashabushi ( Yashabushi or Himeyashabushi or Obayayashabushi `` fruit, peel, ears '', yachiyanagi, yatsude (octagonal metal plate), mistletoe (parasitic willow), willow (kawayanagi, tachinyanagi, weeping willow, red eye willow, pussy willow, inuyanagiunyagi, yinagoyanagina , Yamayama willow, palm, yamayanagi, obanyanagi, tyrichkinuyanagi, foxwood willow, bonsai), yanagitade `` leaves, stems '', yabugarashi, yabukouji (purple gold beef), yabu cigarette (tsuruisui, tenmeisei),
Pokeweed (commercial land), alder (yamaharu), yamamo (yang plum skin), sagebrush, eucalyptus, yukinoshita (tiger ear grass),
Yucca or flevifolia, Yuzu `` fruit '', lily, royal grass, mugwort (Aiba), ligan (Rainuma), lime `` fruit '', rye, lacanca `` fruit '', raspberry `` leaf,
Fruits, Latania (Crameria), Rakkyo or Shallot (Shiroi), Lavender, Longan (Longan flesh), Agave (Aono agave, Fukurin Agave), Ryokucha (green tea), Apple `` Fruits, seeds,
`` Leaves, roots '', gentian, rubus or suavisimus,
Ganoderma litchi (Lichi, Lichi nucleus), lettuce (Chisha), red currant "fruit", lemon "fruit", lemongrass, forsythia or Chinese forsythia (forsythia), astragalus, coconut palm, lokon (reed, azuki), loganberry Examples include "fruit", rosemary (mannen wax), rose hip (nobara), wasabi, and valerobium (chiyu).

In addition, other seawaters such as seawater salt, dried seawater, inorganic salts obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud (fango) Species, for example, sea mud or mud of various places such as Italian Fango, German Fango, Eifel Fango, Freiburg Fango, etc. (Ingredients:
Silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese,
Arsenic, water), Shotoku stone.

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidsa, Chlorella ellipsoidia, Aonori (Usubaonori, Susiaonori, Hiraaonori, Bowaoonori, Hosoedaoonori), and seaweed [brown alga: Kombu algae: , Resilicon b, hosomekobu, mishitoshibu), seaweed, hirome, blue seaweed, giant kelp (macrocystis pilifera, macrocystis integrifolia, neocitys luetokeana), hijiki, hibamata), seaweed [red alga: hijirimen, maxa ( Tengsa),
Hirakusa, Omikusa, Obakusa, Katao Bakusa, Yatabegusa, Yuikiri, Shimanegusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Zyakushinkirinsai, Tsunomata, Obatsunomata, Tochaka , Ibotsunomata, Yaleus Banori, Kagius Banori,
Sujiusbanori, haiusbanori, akaminominori] and the like.

Other algae such as green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Kwanomimo, Volvox: Ohikawawari, Volvox, Palmera, Yotsumemo, Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asiaticum gutta, kawashiogusa, marimo, baronia: tamagobaronia, tamabaronia, tamagata: tamagatamo, iwata: husaiwata, surichogita, heraiwata, kurokita, kurokita, kurokita Sakibuchimil, Nagamir, Hiramil, and Casanori:
Casanori, Jusmo: Fusuzumo, Tamazumi,
Species of the genus Mizudzumo, Genus Genus, Genus Colecaet, Genus Genus genus, Species of the genus Cyperaceae: Species of the genus Cyperaceae, the genus of the spider genus;

Blue-green algae (Genus L .: L., L., L., L .: L., mushrooms,
(Hassay, Yuremo genus, Racenmo (Spirulina) genus: Spirulina, Trichodesmium (Aiacacio) genus, etc.).

Brown algae (Piraella spp .: Piraella spp., Spirogyra spp.
Genus Isogawara: Isogawara, Kuroshira Genus: Gunsenkurogashira, Kashirazaki Genus: Kashirazaki, Muchimo Genus: Muchimo, Hiramutimo, Kebelligsa, Amidixa Genus: Amidigsa, Sakibiroamiji, Sanadagusa: Sanadagusa, Fukurinamiji, Common Genus Artemisia spp.
Genus genus: genus pine, genus genus: genus genus: genus genus, genus okinawa, genus okinawazuku, genus phlebosum, genus phlebosum, genus phlebosum: genus futomozuku, genus genus moss: genus genus: genus kuromo, genus moss, genus genus Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Ursigusa,
Betula serrata, Tobacco moss, Laminaria spp. Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakusugusa: Sharks
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Genus: Genus Genus: Genus Filiformis, Genus Turmo: Genus Tsurumo, Genus Anana: Genus Anastasia, Genus Suzume: Genus Musicum, Genus Missiscoma, Genus L. , Oni-kombu, Gohei-kombu, Naga-kombu, Pea-kombu, Ochimitsu-kombu, Toro-kombu genus: Toro-kombu, Antokume genus: Antokume, Kajime genus: Kajime, Tsuruarame, Kurome, Kikuishicombu genus: Kikuishikombu, Nejirekombu: Nekorecome genus, Kuroshimae : Nekoshikambu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Hokumoku, Tamamomoku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametarawara, Tatsukuri, Yasumatamoku, Umitrano, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Saw-moku, Obanoki-mimoku, Genus genus:
Bulukimo, genus cyno (Kayamonoli etc.).

Red algae (Russia genus: Ushikenori, Funorinoushike, Amanori: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acer genus, Acer genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: Sodegarami, rattles: rattle, rattle rattle, hirohatamatamaki genus: hirohatamaitadaki, tamamaidaki genus: tamaitadaki, kagikenori genus: kaginori, kakenenori, tengusa: himetenugosa, haitenugosa, haitenugosa, haitenugusaugou, haitenuganugou, haitenuganugou, haitenugusaugou Genus: hibiroudo, himehibiroudo,
Genus Isommedogi: Genus Isomomedo, Genus genus: Genus medusa, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarekususa: Genus Nisekaregusa, Okitsubara: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana genus
Naminohana, coral genus: rattle, mushroom, shiogosagoromo, iwanokawa: ezukiiwanokawa, kainokawa: kainokawa, kaninote: kaninote, coral genus: coral, coral, mosquito mosquito , Tambanori, slippery,
Genus Isonohana: Nuracusa, Cronulla, Stinging genus, Hirakintoki: Hirakintoki, Matagawa: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Komenori, Tosakamatsu, Aedes pine, Kakraitus: Genus Odonata, Genus Larva Genus Nagaobane, Funori: Hanafunori, Fukuronori, Makuhnori, Karenikusa: Kalexigusa, Tosakamomoki: Hosobanotokamodoki, Hirohanotosakamakiki, Yatsudegatomodoka, Kurosakacamodoki, Nezashinodoka, Nezashinotokada : Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hanagatakarimenia, Honooka: Hounoo, Hikagenoito: Hikagenoito, Usuginu, Nikuhono: Nikuho Noo, Benisunago genera: Benisunago, Susukakebeni genera: Susukakebeni, Okamuragusa genera:
Yamadakusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagiibanodan, Kijinotho genus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurushiramo, Shiramo, Ogogonori, Mizookonori, Kabanori, Tengusamodoki: Hachijotengusamodoki, Fushikrenori: Fushikenori, Namiiwatake: Namiiwatake, Spiraea:
Sponge and genus: ssp.
Genus: crocodile, crocodile, crocodile, lotus spp .: lotus spp. The genus Taogosou: Taoyanagisou, Hanasakura, Shirotsunagi: Skullconi, Shikokonori, Hananoeda: Hanaeda, Hiratagoat: Hiratagoat, Darusu: Dars, Masagoshibari, Anadars, Weedbugsa: Uebaguni, Benifukunori
Species of sarcophagi: sarcophagia, scorpion, scorpion, scorpion genus: syllabus, smelt, ssp. Family: Habutaenori, Konohanori, Suzushironori, Usbeni genus: Usbeni, Hasuginu: Hasujiginu, Nagakonohanori: Nagakonohanori, Suzuginu: Suzuginu, Abasibaginu, Hyusbanori: Kagusubanori, Jauresubanoli, Jaureusubanoli, Jaureusubanori, Jaureusubanoli
Genus Ayanishi: Genus Ayanishi, Genus Ayaginu: Genus Ayaginu, Genus Dassia: Enashidazia, Genus Shimadagi: Genus Isohagi, Genus Shimadada, Genus Dazimodoki: Genus Dazimomodoki, Genus Itogusa: Moletogusa, Genus Genus: Makuri, Yanagina, Hana, Rana Salix var. Genus Isomegusa: Isomegusa, Suginashigusa: Suginashigusa, Isobashi Genus: Isobashi, Fujimatsumo: Fujimatsumo, Sawweed: Mushroom Rihiba, Kawamozuku genera: Kawamozuku, Aokawamozuku, Himekawamozuku, Ideyukogome genera: Ideyukogome, Okichimozuku genera: Okichimozuku,
Genus genus, genus chinorimo: chinorimo, genus thysinori: chisinori).

The axle algae (Charus spider, Shiratamago,
Hossiturimo: Hossiturimo, Lychnotanus genus, Hessian moss: Himejirasamo, Chaboshiramo, Tripera, etc., and yellow algae (Hikarimo: Hikarimo etc.).

Materials derived from animal raw materials include a cockscomb extract, a cow or human placenta extract, an extract of a stomach, duodenum, or intestine of a pig or cow or a decomposition product thereof, and an extract of a spleen of a pig or cow. Product or its degradation product, extract of pig or cow brain tissue, water-soluble collagen, collagen derivative such as acylated collagen, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its decomposition product Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig or bovine blood cell protein degradation product (globin peptide), a pig or bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.),
Cow milk, casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg components, fish meat decomposition products and the like.

(14) Microbial culture metabolite Yeast metabolite, yeast extract extract, natto metabolite, natto extract, rice ferment extract, rice bran ferment extract, euglena extract, lactic acid bacteria ferment metabolite of raw milk or skim milk powder ( Conventional products) and trehalose or derivatives thereof.

(15) α-hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber / blocker Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-
5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, dihydroxydimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-
Aminobenzoic acid derivatives (such as paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate), and methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, Octyl paramethoxycinnamate, paramethoxycinnamic acid
2-ethoxyethyl, sodium paramethoxycinnamate, potassium paramethoxycinnamate, monodiparamethoxycinnamate
Glyceryl-2-ethylhexanoate), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate, etc.), urocanin Acid derivatives (such as urocanic acid and ethyl urocanate), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, Nicotinic acid derivatives, vitamin B6 derivatives, umbelliferone, esculin, benzyl cinnamate, sinoxate, oxybenzone, diamine Kishibenzon, Okutabenzon, sulisobenzone, benzo resorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, Neo Heliopan, ESKA roll,
Zinc oxide, talc, kaolin, etc.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin C or its derivatives (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or its derivatives, kojic acid or its derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, placenta extract , Ellagic acid, lucinol and so on.

(19) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (eg, magnesium phosphate of vitamin C, vitamin C glucoside), hydroquinone or a derivative thereof (eg, hydroquinone benzyl ether), kojic acid or a derivative thereof, vitamin E or a derivative thereof Derivatives thereof, N-acetyltyrosine or derivatives thereof, glutathione, hydrogen peroxide, zinc peroxide, placental extract, ellagic acid, arbutin, rucinol, silk extract, plant extracts (chamomile, mulberry, mulberry, waremokou, clara, mugwort, Honeysuckle, yellowfin, yellowtail, matsuhodo, pearl barley, scotch, hops, hawthorn, eucalyptus, achillea millefolium, althea, keihi, mankeishi, hamamelis, yamaguwa, enmeisou, bellflower, toshishi, seizome, sun dried, mao, senkyu Dokkatsu, Psycho, storm, littoralis, Scutellaria, peony bark, peony, cranesbill, kudzu root, licorice, Fushi aloe, Cimicifuga, safflower, green tea, black tea, Ali elixir), and the like.

(20) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide, hydroquinone or a derivative thereof (hydroquinone benzyl ether) and the like.

(21) Action of promoting turnover / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (Aloe, Ogon, Horsetail, Gentian, Burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odrich, Assemble, Touki, Calendula officinalis, Amacha, Hypericum perforatum, Cucumber, Periwinkle,
Rosemary, parsley).

(22) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, aluminum potassium sulfate, resorcinol, ferric chloride, tannic acid (including catechin compounds) Such.

(23) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(24) Antioxidants Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, Parahydroxyanisole, propyl gallate, sesamol,
Sesamolin, gossypol, etc.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emmeso, Birch, Sage, Rosemary, Minami Amami,
Ages, ginkgo, green tea) etc.

(26) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor,
dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or a salt thereof, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract and the like.

(27) Antibacterial, bactericidal and disinfecting agents acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or its hydrolyzate, alkyldiaminoethylglycine chloride solution, triclosan, sodium hypochlorite, Chloramine T, salad powder, iodine compound,
Iodoform, sorbic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, o-phenylphenol, irgasan CH3565, halocarban, hexachlorophen, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol, Isopropyl methyl phenol, nonionic surfactants (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene Octylphenyl ether, etc.), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde , Hexamine, brilliant green, malachite green,
Crystal Violet, Jamal, Photosensitizer 101
No. 201, Photosensitizer No. 401, N-long-chain acyl basic amino acid derivatives and their acid addition salts, zinc oxide, hinokitiol, kudin and the like.

(28) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid or its salt, lactic acid Sodium, Mini Sasanishiki extract, Bacillus natto metabolite, Natto extract, etc.

(29) Inhibitors of elastase activity diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, mulberry leaves, calyx, gennoshoko, comfrey, sage, seaweed, bollige, buttonpi), seaweed extracts and the like.

(30) Hair Agent Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid and the like.

(31) Antiandrogens Estrogen (estrone, estradiol, ethinylestradiol, etc.), isoflavones, oxendrones and the like.

(32) Peripheral vascular blood flow promoter Vitamin E or a derivative thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(33) Topical stimulants Pepper tincture, vanylamide nonylate, tincture tincture, tincture tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activator Photosensitizer 301, hinokitiol, pantothenic acid or a derivative thereof, allantoin, placental extract, biotin, pentadecanoic acid glyceride and the like.

(35) Antiseborrheic agents pyridoxine or its derivatives, sulfur, vitamin B6 and the like.

(36) Keratolytic agent Resorcin, salicylic acid, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium silicate, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract, etc.

(38) Hair remover strontium sulfate, sodium sulfide, barium sulfide,
Inorganic reducing agents such as calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) and the like.

(39) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(40) Dye agent 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol ,
2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, ortho Aminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-
Diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol,
Picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenyl Paraphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate,
Orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-sulfate
Diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine , Α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, 2-hydroxy
Sodium 5-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfonate, hematein and the like.

(41) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, Coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Greater Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemon grass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(42) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, preservatives / anti-binders, fresheners, stabilizers, emulsifiers, animal and vegetable proteins and their decomposed products, animal and vegetable Vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory and anti-allergic agents, cell activators, keratolytic agents, wound healing agents, foam enhancers, thickeners Agents, oral agents, deodorants / deodorants, bitters, seasonings, enzymes, and the like can be used, and when used in combination therewith, various additive and synergistic effects can be expected.

Further, the humectant and the immunosuppressant of the present invention include:
It can be used in any form of cosmetics and baths such as capsules, powders, granules, solids, liquids, gels, air bubbles, emulsions, creams, ointments, sheets and the like.

Specifically, basic preparations such as medicinal preparations for external use, lotions, emulsions, creams, ointments, lotions, oils, and packs, facial cleansers and skin cleansers, shampoos, rinses, hair treatments, hair styling products, and permanents It can be applied to various products such as hair cosmetics such as cosmetics, hair tonics, hair dyes, hair growth and hair restoration, foundation cosmetics such as lipstick, blusher, eyeshadow, eyeliner, mascara, perfumes, bath agents, etc. .

The method of adding to the humectant, the immune lowering preventive agent, the cosmetic or the bath agent of the present invention may be added in advance or during the production. What is necessary is just to select suitably.

[0070]

EXAMPLES The present invention will now be described with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Production Example 1) A medium containing 6.0% skim milk powder, 3.3% total milk protein, and 0.1% yeast extract contained La
bulgaricus TY569 strain is inoculated with 1% of ctobacillus delbueckii subsp. Then the culture
After flash sterilization at 80 ° C., the solution was centrifuged to remove insoluble solids, thereby obtaining a solution containing a lactic acid bacteria fermentation metabolite.

(Production Example 2) A medium containing 6.0% of skim milk powder, 3.3% of total milk protein, and 0.1% of yeast extract was mixed with La medium.
bulgaricus TY569 strain is inoculated with 1% of ctobacillus delbueckii subsp. Then the culture
After instantaneously sterilizing at 80 ° C, centrifuge, add 10% ethanol and 0.1% methyl paraoxybenzoate to the supernatant to a concentration of 0.1%, and filter through a membrane filter to obtain a solution containing lactic acid bacteria fermentation metabolites. I got

(Production Example 3) In a medium containing 6.0% of skim milk powder, 3.3% of total milk protein and 0.1% of yeast extract, La was added.
bulgaricus TY569 strain is inoculated with 1% of ctobacillus delbueckii subsp. Then culture at 80 ° C
And then centrifuged, and 1,3-butylene glycol was added to the supernatant to a concentration of 30%, followed by filtration through a membrane filter to obtain a solution containing a fermented metabolite of lactic acid bacteria.

(Test 1) Moisturizing Test (Test Method and Evaluation Method) 1) Using an apparatus for measuring the water content of the stratum corneum of the skin (high frequency IMPERDANCE METER model IB 335, manufactured by IBS Co., Ltd.), an outer diameter of 6 mm was used. The electrical conductivity of the skin was measured with an electrode having a center diameter of 1 mm. The measurement site is the inside of the forearm flexion side, and the electrical conductivity of the measurement site is measured in advance. Next, about 1 mL of each sample was applied to the measurement site, an excess sample was removed after 30 seconds, and the electric conductivity was measured every 30 seconds immediately afterwards for 5 minutes, and a moisture retention test was performed. In addition, the sample of the moisturizing test used the lactic acid fermented liquor (dry matter concentration 1.0%) obtained in Production Examples 1 to 3 of the present invention for the test. The test conditions were as follows: temperature: 22 to 24 ° C., relative humidity: 50 to 52%, and the results are shown in FIG.

(Test Results) As shown in FIG. 1, the fermented metabolite of lactic acid bacteria according to the present invention has a higher moisturizing effect and a longer retention time than the conventional fermented metabolite of lactic acid bacteria of the same concentration (comparative example) and aloe vera extract (comparative example). Turned out to be.

(Test 2) Prevention test for immune decline induced by ultraviolet rays It is known that ultraviolet rays (UV-B) decrease the immune function of the skin. This is thought to be because Langerhans cells (hereinafter referred to as LC cells) in the cells constituting the skin disappear or deteriorate in function due to UV-B irradiation. LC cells have a function of transmitting antigen or cancer cell information to immune system cells (T cells) and are considered to be deeply involved in the contact hypersensitivity reaction (Ueda, 9th SC
CJ Seminar text 10 (1996)). In this study, we investigated the effect of UV-induced contact hypersensitivity on the prevention and improvement of skin immunity (Horio et al .: Nisshin Gakkai 105 (12), 1621).
(1995), Uchida: Fragrance Journal 9, 29 (1991)) as an index.

(Test Method) a. Sample The lactic acid bacteria fermentation metabolite obtained in Production Example 2 was concentrated under reduced pressure by distilling off the solvent under reduced pressure, and a hydrophilic petrolatum ointment containing 10% in terms of dry matter concentration was produced and used by a conventional method. Also,
As a comparative example, a conventional fermented metabolite of lactic acid bacteria was tested. b. Measurement of edema swelling rate That is, ultraviolet light (Toshiba FL as a UV-B lamp)
-20S · E lamp) at 50 mJ / cm ² . Immediately thereafter, the test drug was applied to the irradiated site, and the same was applied the next day and the next two days, and the test was performed three times in total. 6 hours after the final application, 0.2% (W / W) DNFB (1-Fluoro-2,4-dinitrobenzene,
(Wako Pure Chemical was dissolved in acetone: olive oil 4: 1)
25 μL of the solution was applied and sensitized. 7 days after the sensitization, 20 μL of 0.2% DNFB was applied to the auricle of one side of the mouse. In the evaluation method, the left and right auricles were cut out with a punch (about 5 mm in diameter) 24 hours after the eruption, the weight of the cut out auricles was measured, and the difference was defined as the auricle swelling value. In addition, the animals which were not subjected to the ultraviolet irradiation and the test drug and were subjected to only the sensitizing operation (unirradiated group) were provided for comparison. In addition, eight 10-week-old C3H / HeN female mice were used per group.

(Test Results) As a result of observing the inflammation of the contact hypersensitivity reaction caused by the DNFB solution performed in this test method, the group irradiated with ultraviolet rays (control) was more inflamed than the group not irradiated with ultraviolet rays (unirradiated). Showed a tendency to decrease inflammation (decreased skin immune response). However, the group to which the lactic acid bacterium fermentation metabolite of the present invention was applied after irradiation with ultraviolet light was shown in FIG.
As shown, the reduction of inflammation was suppressed. From this,
It was confirmed that the fermented metabolite of lactic acid bacteria of the present invention has a preventive effect on the reduction of immune response due to ultraviolet rays. In addition, skin immunosuppression involves LC cell damage, and the various extracts of the present invention have the effect of maintaining LC cells normally. Furthermore, LC cells are EGF (epidermal cell growth factor), F
It is secreted as GF (fibroblast growth factor) and is indispensable for maintaining the skin structure, suggesting that the fermented metabolite of lactic acid bacteria of the present invention is important for maintaining the skin structure.

(Test 3) Safety test (1) Primary skin irritation test The fermented metabolite of lactic acid bacteria obtained in Production Examples 1 to 3 was purified water so as to have a dry matter concentration of about 5.0 W / V%. Japanese white rabbits (female, 3 per group, weight 2.3k)
g) of the skin. The judgment was made 24, 48, and 72 hours after application by the primary irritation scoring method using erythema and edema as indices. The result was negative in all animals without any erythema and edema.

(Test 4) Safety Test (2) Skin Cumulative Irritation Test Similarly, the fermented metabolite of lactic acid bacteria obtained in Production Examples 1 to 3 was purified water so as to have a dry matter concentration of about 5.0 W / V%. Hartley-type guinea pig (female,
Once a day, 5 times a week on the skin of 3 animals per group, weight around 320g)
0.5 mL / animal was applied. Application was performed over two weeks and shaving was performed on the last application day of each week. The determination was made on the day following each application day and the day after the last application day by using the primary irritancy scoring method with erythema and edema as indices. As a result, all animals were judged negative without any erythema and edema over 2 weeks.

(Test 5) Safety test (3) Acute toxicity test Similarly, the lactic acid bacteria-fermented metabolites obtained in Production Examples 1 to 3 were dried under reduced pressure and dried, and fasted for 4 hours before the test.
2,000m in mice (male and female, 5 mice per group, 5 weeks old)
g / kg was orally administered, and the appearance and degree of toxicity symptoms were observed over time. As a result, no abnormalities were observed in all the mice for 14 days, and no abnormalities were observed in the dissection results. Therefore, the LD50 was determined to be 2,000 mg / kg or more.

(Prescription Examples) Production of Various Cosmetics and Bath Agents According to the above evaluation results, the prescription examples are shown below. The prescription examples may be those produced by a conventional method in the production of each product. showed that. Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion% 1. Squalane 5.0 2. Olive oil 3.0 3. Jojoba oil 3.0 4. Cetyl alcohol 2.0 5. Glycerin monostearate 2.0 6. Polyoxyethylene (20) cetyl ether 3.0 7. Polyoxy Ethylene (20) soorbitan monooleate 2.0 8.1,3-butylene glycol 1.0 9. Glycerin 2.0 10. Lactic acid bacteria fermentation metabolite (Production Example 3) 8.0 11. Appropriate amount of fragrance and preservative 12. Purified water 100

(Formulation Example 2) Peel-off pack% 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Lactic acid bacteria fermentation metabolite (Production example 3) 3.0 7. Appropriate amount of fragrance and preservative 8.Purified water 100 residue

(Formulation Example 3) Cold cream% 1. Salicone beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Almond oil 15.0 5. Borax 0.5 6. Lactic acid bacteria fermentation metabolite (Production example 3) 1.0 9. Fragrance , Preservatives appropriate amount 8. Purified water 100 residue

(Prescription example 4) Body soap% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Fermented metabolite of lactic acid bacteria (Production example 1) 1.5 8. pH adjuster qs 9. Preservative Appropriate amount 7.Residual water to be 100

(Formulation Example 5) Shampoo% 1. Triethanolamine lauryl sulfate 6.0 2. Sodium polyoxyethylene lauryl ether sulfate 15.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.16 Lactobacillus fermentation metabolite (Production Example 3) 1.5 7. Appropriate amount of fragrance and preservatives 8. Residue with purified water 100

(Formulation Example 6) Rinse% 1. Stearyltrimethylammonium chloride 3.0 2. Cetostearyl alcohol 3.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Lactic acid bacteria fermentation metabolite (Production example 3) 1.5 6. pH adjuster qs 7.preservative qs 8.purified water 100

(Formulation Example 7) Hair liquid% 1. Ethanol 30.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Fermentation metabolite of lactic acid bacteria (Production Example 2) 1.0 6.Appropriate amount of preservative 7.Residual water to be 100

(Formulation Example 8) Hair tonic% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Fermented metabolite of lactic acid bacteria (Production Example 2) 1.0 5. Perfume and preservative Appropriate amount 6.Remaining amount of purified water 100

(Formulation Example 9) Granule bath preparation% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 3.0 4. Lactic acid bacteria fermentation metabolite (Production Example 1) 2.0

(Prescription Example 10) Coating protective agent A gauze or liniment cloth is impregnated with a prescription liquid prepared by mixing a fermented metabolite of lactic acid bacteria (Production Example 3), an antibiotic, an anti-inflammatory and the like in an appropriate amount, and attached to the wound. Alternatively, the fermented metabolite of lactic acid bacteria may be directly applied locally and covered with gauze or the like.

(Test 6) Use effect test The effect of the cosmetic of the present invention actually used was examined. The usage test was conducted for a person suffering from rough and dry skin, and a person with no gloss or tension on the skin.
Was applied to the face and hands over 3 months. As a control, a mixture prepared by removing the lactic acid-metabolized fermented product of the present invention from a milk by the same method was used. The evaluation was performed according to the following criteria.
The numbers in the table represent the number of persons as shown in FIGS. No one complained of abnormal skin during the use period.

"Beautiful skin / moisturizing effect" Effective: Rough skin and dry skin were improved. Slightly effective: rough and dry skin slightly improved. Ineffective: No change from before use.

[0095]

"Skin feel improving effect" Efficacy: Skin gloss and tension increased, and skin was improved. Slightly effective: Skin gloss and tension increased slightly, and skin was improved. Ineffective: No change from before use.

[0097]

(Test Results) As shown in Tables 1 and 2, it was confirmed that the fermented metabolite of the lactic acid bacterium of the present invention significantly improved the rough and dry skin, and gave the skin a gloss and firmness.

[0099]

EFFECT OF THE INVENTION The cosmetics and bath preparations containing the fermented metabolite of lactic acid bacteria of the present invention have a moisturizing action and / or an immunopreventive action, and further prevent or improve rough and dry skin, or provide a glossy skin. You can give it tension. In addition, skin immunosuppression is closely related to LC cell damage, and the fermented metabolite of lactic acid bacteria of the present invention has an effect of maintaining LC cells normally, and is safe for humans and animals.

[Brief description of the drawings]

FIG. 1 is a graph showing the water content of the stratum corneum of a fermented metabolite of lactic acid bacteria of the present invention.

FIG. 2 is a diagram showing the preventive effect of the fermented metabolite of lactic acid bacteria of the present invention on immune reduction induced by ultraviolet light.

────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] April 4, 2000 (200.4.4)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0007

[Correction method] Change

[Correction contents]

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The “fermentation metabolite of a novel lactic acid bacterium (hereinafter referred to as a lactic acid bacteria fermentation metabolite)” used in the present invention is:
Lactic acid bacteria Lactobacillus delbrueckii bulgaricus TY569 (Lactobacillus delbrueckii subsp.bulgar) on a culture medium mainly composed of milk raw materials such as raw milk or skim milk powder
icus TY569) (FERM P-17488), which is obtained by inoculation culture. The lactic acid bacterium has the following bacteriological properties. Bacterial morphology when cultured in MRS liquid medium at an initial pH of 5.6 at 37 ° C for 18 hours (1) Bacterial bacillus (2) Gram stain positive (3) Motility None (4) Spores None (5) Catalase None (6) Facultative anaerobic (7) Growth temperature range 30-45 ° C (8) Growth pH range 5.0-7.5 (9) Lactic acid fermentation homo-type (10) Lactic acid illuminating D (-) (11) Sugar Glucose, fructose, mannose and lactose are positive. In the present invention, those obtained by separating and removing an individual from the culture in the title, or those extracted or prepared with a solvent can be used.

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0071

[Correction method] Change

[Correction contents]

(Production Example 1) A medium containing 6.0% skim milk powder, 3.3% total milk protein, and 0.1% yeast extract contained La
Inoculating 1% of ctobacillus delbrueckii subsp.bulgaricus TY569, and culturing at 40 ° C. for 3 days. Then the culture
After flash sterilization at 80 ° C., the solution was centrifuged to remove insoluble solids, thereby obtaining a solution containing a lactic acid bacteria fermentation metabolite.

[Procedure amendment 3]

[Document name to be amended] Statement

[Correction target item name] 0072

[Correction method] Change

[Correction contents]

(Production Example 2) A medium containing 6.0% of skim milk powder, 3.3% of total milk protein, and 0.1% of yeast extract was mixed with La medium.
Inoculating 1% of ctobacillus delbrueckii subsp.bulgaricus TY569, and culturing at 40 ° C. for 3 days. Then the culture
After instantaneously sterilizing at 80 ° C, centrifuge, add 10% ethanol and 0.1% methyl paraoxybenzoate to the supernatant to a concentration of 0.1%, and filter through a membrane filter to obtain a solution containing lactic acid bacteria fermentation metabolites. I got

[Procedure amendment 4]

[Document name to be amended] Statement

[Correction target item name] 0073

[Correction method] Change

[Correction contents]

(Production Example 3) In a medium containing 6.0% of skim milk powder, 3.3% of total milk protein and 0.1% of yeast extract, La was added.
Inoculate 1% of ctobacillus delbrueckii subsp.bulgaricus TY569 and incubate at 40 ° C for 3 days. Then culture at 80 ° C
And then centrifuged, and 1,3-butylene glycol was added to the supernatant to a concentration of 30%, followed by filtration through a membrane filter to obtain a solution containing a fermented metabolite of lactic acid bacteria.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 7/075 A61K 7/075 7/08 7/08 7/50 7/50 35/74 35/74 G A61P 17/16 A61P 17/16 37/04 37/04 // C12P 1/04 C12P 1/04 Z (C12N 1/20 (C12N 1/20 E C12R 1: 225) C12R 1: 225) (C12P 1 / 04 (C12P 1/04 Z C12R 1: 225) C12R 1: 225) (72) Inventor Kohei Hasebe 318-1 Asagi, Masamasa-cho, Motosu-gun, Gifu Prefecture Inside Ichimaru Falcos Co., Ltd. (72) Inventor Kiyotaka Tanaka Gifu Prefecture 318 Ichimaru Falcos Co., Ltd., 318, Masaki, Masamitsu-cho, Motosu-gun F-term (reference) 4B064 AH19 CA02 DA20 4B065 AA30X CA50 4C083 AA031 AA032 AA082 AA122 AB152 AB312 AB352 AC022 AC072 AC102 AC122 AC182 AC242 AC352 AC422 AC432 AC442 AC442 32 AC542 AC642 AC692 AC782 AC902 AD042 AD112 AD512 BB51 CC01 CC05 CC07 CC23 CC25 CC33 CC38 CC39 DD22 DD23 DD27 DD31 EE06 EE10 EE12 EE13 EE42 FF01 4C087 AA01 AA02 BC56 CA10 MA32 MA63 NA14 ZA91 ZA92 ZB09

Claims (4)

[Claims] 1. A novel lactic acid bacterium Lactobacillus delbrukki vulgarica TY569 (FER) having the following mycological properties and producing an effective ingredient for cosmetic use:
MP-17488). Bacterial morphology when cultured in MRS liquid medium at an initial pH of 5.6 at 37 ° C for 18 hours (1) Bacterial bacillus (2) Gram stain positive (3) Motility None (4) Spores None (5) Catalase None (6) Facultative anaerobic (7) Growth temperature range 30 to 45 ° C (8) Growth pH range 5.0 to 7.5 (9) Lactic acid fermentation homo-type (10) Lactic acid illuminating D (-) (11) Sugar Glucose, fructose, mannose and lactose are positive 2. A humectant for cosmetics comprising the fermented metabolite of the novel lactic acid bacterium according to claim 1 as an active ingredient. 3. An agent for preventing immunosuppression for cosmetics, comprising the fermented metabolite of the novel lactic acid bacterium according to claim 1 as an active ingredient. 4. A cosmetic or bath preparation containing the fermented metabolite of the novel lactic acid bacterium according to claim 1.