JP2000515385A5 - - Google Patents

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Publication number
JP2000515385A5
JP2000515385A5 JP1998508130A JP50813098A JP2000515385A5 JP 2000515385 A5 JP2000515385 A5 JP 2000515385A5 JP 1998508130 A JP1998508130 A JP 1998508130A JP 50813098 A JP50813098 A JP 50813098A JP 2000515385 A5 JP2000515385 A5 JP 2000515385A5
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JP
Japan
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JP1998508130A
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English (en)
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JP2000515385A (ja
JP3905132B2 (ja
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Priority claimed from US08/692,590 external-priority patent/US5707812A/en
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Publication of JP2000515385A publication Critical patent/JP2000515385A/ja
Publication of JP2000515385A5 publication Critical patent/JP2000515385A5/ja
Application granted granted Critical
Publication of JP3905132B2 publication Critical patent/JP3905132B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Figure 2000515385
Figure 2000515385
Figure 2000515385
JP50813098A 1996-08-06 1997-08-05 Peg存在下でのカラムクロマトグラフィーを使用するプラスミドdnaの精製 Expired - Fee Related JP3905132B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/692,590 US5707812A (en) 1996-08-06 1996-08-06 Purification of plasmid DNA during column chromatography
US08/692,590 1996-08-06
PCT/US1997/013647 WO1998005767A1 (en) 1996-08-06 1997-08-05 Purification of plasmid dna by peg-precipitation and column chromatography

Publications (3)

Publication Number Publication Date
JP2000515385A JP2000515385A (ja) 2000-11-21
JP2000515385A5 true JP2000515385A5 (ja) 2005-04-07
JP3905132B2 JP3905132B2 (ja) 2007-04-18

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Family Applications (1)

Application Number Title Priority Date Filing Date
JP50813098A Expired - Fee Related JP3905132B2 (ja) 1996-08-06 1997-08-05 Peg存在下でのカラムクロマトグラフィーを使用するプラスミドdnaの精製

Country Status (7)

Country Link
US (1) US5707812A (ja)
EP (1) EP0920497B1 (ja)
JP (1) JP3905132B2 (ja)
AT (1) ATE287948T1 (ja)
CA (1) CA2262820C (ja)
DE (1) DE69732360T2 (ja)
WO (1) WO1998005767A1 (ja)

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US20030045492A1 (en) * 1997-08-13 2003-03-06 Tang De-Chu C. Vaccination by topical application of recombinant vectors
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US20040009936A1 (en) * 1999-05-03 2004-01-15 Tang De-Chu C. Vaccine and drug delivery by topical application of vectors and vector extracts
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US20020010145A1 (en) * 1999-07-12 2002-01-24 Willson Richard C. Apparatus, methods and compositions for biotechnical separations
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DE10006591B4 (de) * 2000-02-11 2007-03-29 Eppendorf Ag Verfahren zur Aufreinigung von Nukleinsäuren
PT102491B (pt) 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
US7074333B2 (en) * 2001-01-19 2006-07-11 Millipore Corporation Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration
DE10113815A1 (de) * 2001-03-21 2002-10-02 Eppendorf Ag Verfahren zur Isolierung von Plasmiden oder Proteinen aus suspendierten Bakterien- oder Hefezellen
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
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KR100502473B1 (ko) * 2001-11-08 2005-07-20 동아제약주식회사 플라스미드 dna의 분리방법
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내dna 또는 rna로부터 리포폴리사카라이드를제거하는 방법
US6833238B2 (en) * 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
US20040018559A1 (en) * 2002-07-26 2004-01-29 Applera Corporation Size-exclusion ion-exchange particles
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
AU2003293042A1 (en) * 2002-12-23 2004-07-29 Vical Incorporated Process for purification of plasmid dna
WO2004108260A2 (en) * 2003-05-30 2004-12-16 Advisys, Inc. Devices and methods for biomaterial production
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
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US7504243B2 (en) 2004-03-19 2009-03-17 The United States Of America As Represented By The Department Of Health And Human Services Methods for the production of biliverdin
US7527929B2 (en) 2004-07-30 2009-05-05 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
EP1856262B1 (en) 2005-01-31 2012-08-15 Merck Sharp & Dohme Corp. Upstream and a downstream purification process for large scale production of plasmid dna
ATE476505T1 (de) * 2005-02-11 2010-08-15 Invitrogen Dynal As Verfahren zur isolierung von nukleinsäuren mit verwendung von ethylenglykol-multimeren
US7524510B2 (en) * 2005-02-23 2009-04-28 The Uab Research Foundation Alkyl-glycoside enhanced vaccination
CA2604909A1 (en) * 2005-04-15 2006-10-26 The Government Of The United States Of America, As Represented By The Se Cretary, Department Of Health And Human Services Methods and compositions for producing an enhanced immune response to a human papillomavirus immunogen
WO2007065025A2 (en) * 2005-11-29 2007-06-07 Wisconsin Alumni Research Foundation Method of dna analysis using micro/nanochannel
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
GB2445441B (en) * 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
WO2008150826A1 (en) * 2007-05-31 2008-12-11 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
US20090227011A1 (en) * 2008-02-29 2009-09-10 Shizhong Chen Methods of purifying plasmid dna
WO2014059188A1 (en) 2012-10-12 2014-04-17 Sage Science, Inc. Side-eluting molecular fractionator
JP6381628B2 (ja) 2013-03-18 2018-08-29 キアゲン ゲーエムベーハー 生物試料の安定化
CA2964487C (en) 2014-10-15 2024-03-19 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
US20170239658A1 (en) * 2014-10-16 2017-08-24 Ezra S. Abrams Methods and systems for controlling the shear modulus of genomic length dsdna molecules
CN108699101B (zh) 2015-11-20 2022-04-19 塞奇科学股份有限公司 用于基因组dna片段的靶向纯化的制备型电泳方法
AU2018250330A1 (en) 2017-04-07 2019-09-19 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic DNA purification
SG11202112350XA (en) * 2019-05-15 2021-12-30 Translate Bio Inc Methods for purification of messenger rna
CN115612684A (zh) * 2022-11-01 2023-01-17 北京启辰生生物科技有限公司 Gmp级质粒dna大规模简化生产方法

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JPH01304886A (ja) * 1988-05-31 1989-12-08 Sekisui Chem Co Ltd Dnaの精製方法
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA

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