JP2000503109A - 糖タンパク質のグリコシル化の特性表示方法および糖タンパク質の生物学的利用能のインビトロ測定方法 - Google Patents
糖タンパク質のグリコシル化の特性表示方法および糖タンパク質の生物学的利用能のインビトロ測定方法Info
- Publication number
- JP2000503109A JP2000503109A JP9507136A JP50713697A JP2000503109A JP 2000503109 A JP2000503109 A JP 2000503109A JP 9507136 A JP9507136 A JP 9507136A JP 50713697 A JP50713697 A JP 50713697A JP 2000503109 A JP2000503109 A JP 2000503109A
- Authority
- JP
- Japan
- Prior art keywords
- glycoproteins
- glycoprotein
- glycan
- charge
- glycosylation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.仮説電荷数(N)を用いて糖タンパク質のグリコシル化の特性を表示する方法 において、電荷数は、 a)糖タンパク質のグリカンプールを単離し、 b)そのプールを、イオン交換クロマトグラフィーにより、主として電荷に 応じて分画化し、 c)電荷に応じて分離されたピーク群(グリカン)の面積によって表される %分画を求め、 d)中性(アシアロ)、モノシアロ(MS)、ジシアロ(DiS)、トリシアロ(T riS)、テトラシアロ(TetraS)およびペンタシアロ(PentaS)範囲におけるピ ーク群の面積によって表される%分画にそれぞれ0(アシアロ)、1(MS)、2 (DiS)、3(TriS)、4(TetraS)および5(PentaS)を乗じ、ついで e)各場合に得られる積を合計する ことによって決定する方法。 2.請求項1記載の方法を用いて糖タンパク質について得られた電荷数をインビ ボ測定で得られた標準値と関連づける、糖タンパク質の生物学的利用能のインビ トロ測定方法。 3.請求項1記載の方法を用いて糖タンパク質について得られた電荷数を計算で 得られた標準値と関連づける、糖タンパク質の生物学的利用能のインビトロ測定 方法。 4.請求項1記載の方法を用いて糖タンパク質について得られた電荷数を請求項 1記載の方法を用いて標準プレパレーションについて得られた標準値と関連づけ る、糖タンパク質の品質同一性を測定する方法。 5.請求項1記載の方法を用いて糖タンパク質について得られた電荷数を 計算で得られた標準値と関連づける糖タンパク質の品質同一性を測定する方法。 6.グリカンプールは糖タンパク質から加ヒドラジン分解によって遊離させる請 求項1記載の方法。 7.グリカンプールは糖タンパク質から酵素的に(PNGアーゼFを用いて)遊離 させる請求項1記載の方法。 8.イオン交換クロマトグラフィーはHPAE-PAD(パルス電流滴定検出による高− pH陰イオン交換クロマトグラフィー)である請求項1記載の方法。 9.糖タンパク質のグリコシル化状態の特徴を表示する請求項1記載の方法。 10.糖タンパク質の生物学的利用能をチェックする請求項2または3記載の方法 。 11.細胞工学技術によって製造された糖タンパク質の品質同一性をチェックする 請求項4または5記載の方法。 12.診断補助剤としておよび/または診断薬としての請求項1記載の方法の使用 。 13.疾患に特徴的な糖タンパク質のグリコシル化状態(電荷数N)によって種の 疾患状態をチェックする請求項1記載の方法。 14.疾患に特徴的な糖タンパク質のグリコシル化状態(電荷数N)によって患者 の疾患状態をチェックする請求項1記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19527054A DE19527054A1 (de) | 1995-07-26 | 1995-07-26 | Verfahren zur Charakterisierung der Glycosylierung von Glycoproteinen sowie zur in-vitro Bestimmung der Bioverfügbarkeit von Glycoproteinen |
DE19527054.1 | 1995-07-26 | ||
PCT/EP1996/002319 WO1997005490A1 (de) | 1995-07-26 | 1996-05-30 | Verfahren zur charakterisierung der glycosylierung von glycoproteinen sowie zur in-vitro bestimmung der bioverfügbarkeit von glycoproteinen |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2000503109A true JP2000503109A (ja) | 2000-03-14 |
JP2000503109A5 JP2000503109A5 (ja) | 2004-07-15 |
JP3631495B2 JP3631495B2 (ja) | 2005-03-23 |
Family
ID=7767674
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50713697A Expired - Fee Related JP3631495B2 (ja) | 1995-07-26 | 1996-05-30 | 糖タンパク質のグリコシル化の特性表示方法および糖タンパク質の生物学的利用能のインビトロ測定方法 |
Country Status (13)
Country | Link |
---|---|
US (1) | US6096555A (ja) |
EP (1) | EP0843821B1 (ja) |
JP (1) | JP3631495B2 (ja) |
KR (1) | KR19990035943A (ja) |
AT (1) | ATE206524T1 (ja) |
AU (1) | AU6003296A (ja) |
CA (1) | CA2227743C (ja) |
DE (2) | DE19527054A1 (ja) |
DK (1) | DK0843821T3 (ja) |
ES (1) | ES2162652T3 (ja) |
NO (1) | NO980276L (ja) |
PT (1) | PT843821E (ja) |
WO (1) | WO1997005490A1 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004271273A (ja) * | 2003-03-06 | 2004-09-30 | Japan Science & Technology Agency | 高等植物の糖リン酸の網羅的分析法 |
JP2012513030A (ja) * | 2008-12-19 | 2012-06-07 | モメンタ ファーマシューティカルズ インコーポレイテッド | 修飾グリカンに関する方法 |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020144310A1 (en) * | 2000-01-28 | 2002-10-03 | Lightfoot David A. | Isolated polynucleotides and polypeptides relating to loci underlying resistance to soybean cyst nematode and soybean sudden death syndrome and methods employing same |
US20030096281A1 (en) * | 2001-09-14 | 2003-05-22 | Ganesh Venkataraman | Methods of making glycomolecules with enhanced activities and uses thereof |
KR100982922B1 (ko) * | 2002-01-17 | 2010-09-20 | 론자 바이올로직스 피엘씨 | 단백질을 생산할 수 있으며 무글루타민 배지에서 성장할 수있는 글루타민-요구성 인간세포 |
EP1587408A4 (en) * | 2002-12-20 | 2007-09-05 | Momenta Pharmaceuticals Inc | GLYCAN MARKER FOR DIAGNOSIS AND MONITORING OF DISEASES |
DE102005050580A1 (de) * | 2005-10-21 | 2007-04-26 | Siegfried Biologics Gmbh | Verfahren zur Charakterisierung der Qualität von Sialoglycoproteinen über eine Isoformenzahl I und dessen Verwendung |
AU2011237442A1 (en) | 2010-04-07 | 2012-10-18 | Momenta Pharmaceuticals, Inc. | High mannose glycans |
US9170249B2 (en) | 2011-03-12 | 2015-10-27 | Momenta Pharmaceuticals, Inc. | N-acetylhexosamine-containing N-glycans in glycoprotein products |
US20160145589A1 (en) | 2011-06-24 | 2016-05-26 | Green Cross Corporation | Composition and formulation comprising recombinant human iduronate-2-sulfatase and preparation method thereof |
WO2013181575A2 (en) | 2012-06-01 | 2013-12-05 | Momenta Pharmaceuticals, Inc. | Methods related to denosumab |
KR101380740B1 (ko) * | 2012-06-29 | 2014-04-11 | 쉐어 휴먼 제네텍 세러피스, 인코포레이티드 | 이듀로네이트-2-설파타제의 정제 |
US9150841B2 (en) | 2012-06-29 | 2015-10-06 | Shire Human Genetic Therapies, Inc. | Cells for producing recombinant iduronate-2-sulfatase |
US10450361B2 (en) | 2013-03-15 | 2019-10-22 | Momenta Pharmaceuticals, Inc. | Methods related to CTLA4-Fc fusion proteins |
WO2014186310A1 (en) | 2013-05-13 | 2014-11-20 | Momenta Pharmaceuticals, Inc. | Methods for the treatment of neurodegeneration |
WO2015057622A1 (en) | 2013-10-16 | 2015-04-23 | Momenta Pharmaceuticals, Inc. | Sialylated glycoproteins |
-
1995
- 1995-07-26 DE DE19527054A patent/DE19527054A1/de not_active Withdrawn
-
1996
- 1996-05-30 EP EP96917465A patent/EP0843821B1/de not_active Expired - Lifetime
- 1996-05-30 US US09/000,307 patent/US6096555A/en not_active Expired - Lifetime
- 1996-05-30 DK DK96917465T patent/DK0843821T3/da active
- 1996-05-30 CA CA002227743A patent/CA2227743C/en not_active Expired - Lifetime
- 1996-05-30 AU AU60032/96A patent/AU6003296A/en not_active Abandoned
- 1996-05-30 PT PT96917465T patent/PT843821E/pt unknown
- 1996-05-30 AT AT96917465T patent/ATE206524T1/de active
- 1996-05-30 WO PCT/EP1996/002319 patent/WO1997005490A1/de not_active Application Discontinuation
- 1996-05-30 KR KR1019980700604A patent/KR19990035943A/ko not_active Application Discontinuation
- 1996-05-30 DE DE59607840T patent/DE59607840D1/de not_active Expired - Lifetime
- 1996-05-30 ES ES96917465T patent/ES2162652T3/es not_active Expired - Lifetime
- 1996-05-30 JP JP50713697A patent/JP3631495B2/ja not_active Expired - Fee Related
-
1998
- 1998-01-21 NO NO980276A patent/NO980276L/no not_active Application Discontinuation
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004271273A (ja) * | 2003-03-06 | 2004-09-30 | Japan Science & Technology Agency | 高等植物の糖リン酸の網羅的分析法 |
JP2012513030A (ja) * | 2008-12-19 | 2012-06-07 | モメンタ ファーマシューティカルズ インコーポレイテッド | 修飾グリカンに関する方法 |
Also Published As
Publication number | Publication date |
---|---|
DE59607840D1 (de) | 2001-11-08 |
DK0843821T3 (da) | 2002-01-28 |
ES2162652T3 (es) | 2002-01-01 |
NO980276D0 (no) | 1998-01-21 |
PT843821E (pt) | 2002-03-28 |
KR19990035943A (ko) | 1999-05-25 |
CA2227743A1 (en) | 1997-02-13 |
AU6003296A (en) | 1997-02-26 |
CA2227743C (en) | 2008-01-29 |
EP0843821A1 (de) | 1998-05-27 |
US6096555A (en) | 2000-08-01 |
EP0843821B1 (de) | 2001-10-04 |
WO1997005490A1 (de) | 1997-02-13 |
MX9800690A (es) | 1998-09-30 |
NO980276L (no) | 1998-01-21 |
ATE206524T1 (de) | 2001-10-15 |
JP3631495B2 (ja) | 2005-03-23 |
DE19527054A1 (de) | 1997-01-30 |
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