IT1271005B - Separazione di acidi nucleici per elettroforesi capillare in gradienti termici e in soluzioni viscose di polimeri - Google Patents

Separazione di acidi nucleici per elettroforesi capillare in gradienti termici e in soluzioni viscose di polimeri

Info

Publication number
IT1271005B
IT1271005B ITMI941864A ITMI941864A IT1271005B IT 1271005 B IT1271005 B IT 1271005B IT MI941864 A ITMI941864 A IT MI941864A IT MI941864 A ITMI941864 A IT MI941864A IT 1271005 B IT1271005 B IT 1271005B
Authority
IT
Italy
Prior art keywords
polymers
separation
thermal gradients
viscous solutions
nucleic acids
Prior art date
Application number
ITMI941864A
Other languages
English (en)
Inventor
Pier Giorgio Righetti
Cecilia Gelfi
Original Assignee
Pier Giorgio Righetti
Cecilia Gelfi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pier Giorgio Righetti, Cecilia Gelfi filed Critical Pier Giorgio Righetti
Priority to ITMI941864A priority Critical patent/IT1271005B/it
Publication of ITMI941864A0 publication Critical patent/ITMI941864A0/it
Priority to AU35652/95A priority patent/AU689648B2/en
Priority to EP95932707A priority patent/EP0781411A1/en
Priority to PCT/EP1995/003561 priority patent/WO1996008715A1/en
Priority to JP8509892A priority patent/JP3015106B2/ja
Publication of ITMI941864A1 publication Critical patent/ITMI941864A1/it
Application granted granted Critical
Publication of IT1271005B publication Critical patent/IT1271005B/it

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44734—Arrangements for investigating the separated zones, e.g. localising zones by thermal means

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

La presente invenzione si riferisce all'uso di gradienti termici nel tempo, per la separazione di frammenti di DNA, amplificati per PCR, sia normali che contenenti mutazioni puntiformi, in elettroforesi zonale capillare in presenza di soluzioni viscose di polimeri, il controllo della temperatura del capillare avendo luogo tramite l'uso di software dedicato, con rivelazione dei frammenti di DNA tramite eccitazione di fluorescenza a raggio laser. E' altresì possibile operare con soluzioni di poliacrilamidi in particolare costituite da monomeri N - sostituiti, quali N, N' - dimetil acrilamide e N - acriloil amino etossi etanolo.
ITMI941864A 1994-09-12 1994-09-12 Separazione di acidi nucleici per elettroforesi capillare in gradienti termici e in soluzioni viscose di polimeri IT1271005B (it)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ITMI941864A IT1271005B (it) 1994-09-12 1994-09-12 Separazione di acidi nucleici per elettroforesi capillare in gradienti termici e in soluzioni viscose di polimeri
AU35652/95A AU689648B2 (en) 1994-09-12 1995-09-11 Separation of nucleic acids by capillary electrophoresis in thermal gradients in viscous polymer solutions
EP95932707A EP0781411A1 (en) 1994-09-12 1995-09-11 Separation of nucleic acids by capillary electrophoresis in thermal gradients in viscous polymer solutions
PCT/EP1995/003561 WO1996008715A1 (en) 1994-09-12 1995-09-11 Separation of nucleic acids by capillary electrophoresis in thermal gradients in viscous polymer solutions
JP8509892A JP3015106B2 (ja) 1994-09-12 1995-09-11 粘性ポリマー溶液中の温度勾配でのキャピラリー電気泳動による核酸の分離

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI941864A IT1271005B (it) 1994-09-12 1994-09-12 Separazione di acidi nucleici per elettroforesi capillare in gradienti termici e in soluzioni viscose di polimeri

Publications (3)

Publication Number Publication Date
ITMI941864A0 ITMI941864A0 (it) 1994-09-12
ITMI941864A1 ITMI941864A1 (it) 1996-03-12
IT1271005B true IT1271005B (it) 1997-05-26

Family

ID=11369544

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI941864A IT1271005B (it) 1994-09-12 1994-09-12 Separazione di acidi nucleici per elettroforesi capillare in gradienti termici e in soluzioni viscose di polimeri

Country Status (5)

Country Link
EP (1) EP0781411A1 (it)
JP (1) JP3015106B2 (it)
AU (1) AU689648B2 (it)
IT (1) IT1271005B (it)
WO (1) WO1996008715A1 (it)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9912743D0 (en) * 1999-06-02 1999-08-04 Proteome Sciences Plc Method and kit for ligand assay
JP2003532073A (ja) * 2000-04-25 2003-10-28 スペクトラメディックス コーポレイション 高温条件下での生体分子の変性剤−フリー電気泳動
WO2002061410A1 (en) 2000-09-01 2002-08-08 Spectrumedix Corporation System and method for temperature gradient capillary electrophoresis
RU2180229C1 (ru) * 2001-01-19 2002-03-10 ООО Научно-производственное фармацевтическое предприятие "Полидан" Способ получения растворов натрия нуклеоспермата
US6872530B2 (en) 2002-04-24 2005-03-29 Spectrumedix, Llc Method for determining the presence of DNA variants using peptide nucleic acid probes
US7303879B2 (en) 2003-07-31 2007-12-04 Applera Corporation Determination of SNP allelic frequencies using temperature gradient electrophoresis
KR20060105787A (ko) 2003-11-21 2006-10-11 가부시키가이샤 에바라 세이사꾸쇼 액체를 이용한 마이크로칩 장치
EP1760159A1 (en) * 2005-08-31 2007-03-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. A method of electrophoresis of nucleic acid molecules
JP6992385B2 (ja) * 2017-10-02 2022-01-13 株式会社島津製作所 電気泳動用分離媒体、電気泳動用試薬キット、及び電気泳動方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3023793B2 (ja) * 1988-02-16 2000-03-21 アプライド バイオシステムズ インコーポレイテッド 毛管電気泳動方法及びその装置
ATE145010T1 (de) * 1989-08-19 1996-11-15 Qiagen Gmbh Verfahren und vorrichtung zur trennung und detektion von komponenten eines stoffgemisches durch temperaturgradienten-gelelektrophorese
US5066382A (en) * 1990-01-25 1991-11-19 Spectra-Physics, Inc. Thermal control for capillary electrophoresis apparatus

Also Published As

Publication number Publication date
EP0781411A1 (en) 1997-07-02
JPH10502738A (ja) 1998-03-10
WO1996008715A1 (en) 1996-03-21
AU3565295A (en) 1996-03-29
JP3015106B2 (ja) 2000-03-06
ITMI941864A1 (it) 1996-03-12
ITMI941864A0 (it) 1994-09-12
AU689648B2 (en) 1998-04-02

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Effective date: 19970926