IT1244983B - Procedimento per rivelare sequenze di acidi nucleici e kit per la sua utilizzazione. - Google Patents

Procedimento per rivelare sequenze di acidi nucleici e kit per la sua utilizzazione.

Info

Publication number
IT1244983B
IT1244983B ITRM910286A ITRM910286A IT1244983B IT 1244983 B IT1244983 B IT 1244983B IT RM910286 A ITRM910286 A IT RM910286A IT RM910286 A ITRM910286 A IT RM910286A IT 1244983 B IT1244983 B IT 1244983B
Authority
IT
Italy
Prior art keywords
procedure
kits
detecting
nucleic acids
probe
Prior art date
Application number
ITRM910286A
Other languages
English (en)
Inventor
Giorgio Martinazzo
Stanislao Marcolini
Roberta Bichi
Elisabetta Turchetti
Original Assignee
Raggio Italgene Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Raggio Italgene Spa filed Critical Raggio Italgene Spa
Priority to ITRM910286A priority Critical patent/IT1244983B/it
Publication of ITRM910286A0 publication Critical patent/ITRM910286A0/it
Priority to PCT/EP1992/000929 priority patent/WO1992019775A1/en
Priority to EP92909161A priority patent/EP0583284A1/en
Priority to AU16499/92A priority patent/AU666648B2/en
Priority to CA002109518A priority patent/CA2109518A1/en
Priority to JP4508516A priority patent/JPH06509464A/ja
Publication of ITRM910286A1 publication Critical patent/ITRM910286A1/it
Application granted granted Critical
Publication of IT1244983B publication Critical patent/IT1244983B/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

E' descritto un procedimento per rivelare sequenze di acidi nucleici per ibridazione in fase omogenea che utilizzi almeno due oligonucleotidi, uno come sonda separatrice ed uno come sonda rivelatrice. Il procedimento è da utilizzare in tutti quei casi in cui sia necessario una doppia selezione per determinare i segnali falsi positivi ed è particolarmente adatto per rivelare segmenti di DNA contigui provenienti da cromosomi diversi o da zone diverse dello stesso cromosoma in seguito a fenomeni di traslocazione cromosomica, trasposizione, ricombinazione intergenica o intragenica, inserzione.la presente invenzione riguarda anche corredi (kits) diagnostici per attuare detto procedimento da utilizzarsi anche per la diagnostica clinica.
ITRM910286A 1991-04-29 1991-04-29 Procedimento per rivelare sequenze di acidi nucleici e kit per la sua utilizzazione. IT1244983B (it)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ITRM910286A IT1244983B (it) 1991-04-29 1991-04-29 Procedimento per rivelare sequenze di acidi nucleici e kit per la sua utilizzazione.
PCT/EP1992/000929 WO1992019775A1 (en) 1991-04-29 1992-04-29 Assay and kit for the detection of chromosomal abnormalities
EP92909161A EP0583284A1 (en) 1991-04-29 1992-04-29 Assay for the detection of chromosomal abnormalities
AU16499/92A AU666648B2 (en) 1991-04-29 1992-04-29 Assay and kit for the detection of chromosomal abnormalities
CA002109518A CA2109518A1 (en) 1991-04-29 1992-04-29 Assay and kit for the detection of chromosomal abnormalities
JP4508516A JPH06509464A (ja) 1991-04-29 1992-04-29 染色体異常の検出用アッセイおよびキット

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITRM910286A IT1244983B (it) 1991-04-29 1991-04-29 Procedimento per rivelare sequenze di acidi nucleici e kit per la sua utilizzazione.

Publications (3)

Publication Number Publication Date
ITRM910286A0 ITRM910286A0 (it) 1991-04-29
ITRM910286A1 ITRM910286A1 (it) 1992-10-29
IT1244983B true IT1244983B (it) 1994-09-13

Family

ID=11400116

Family Applications (1)

Application Number Title Priority Date Filing Date
ITRM910286A IT1244983B (it) 1991-04-29 1991-04-29 Procedimento per rivelare sequenze di acidi nucleici e kit per la sua utilizzazione.

Country Status (6)

Country Link
EP (1) EP0583284A1 (it)
JP (1) JPH06509464A (it)
AU (1) AU666648B2 (it)
CA (1) CA2109518A1 (it)
IT (1) IT1244983B (it)
WO (1) WO1992019775A1 (it)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5015568A (en) * 1986-07-09 1991-05-14 The Wistar Institute Diagnostic methods for detecting lymphomas in humans
WO1995011990A1 (en) * 1993-10-29 1995-05-04 Raggio-Italgene S.P.A. Assay for the detection of genetic abnormalities
US7175995B1 (en) 1994-10-27 2007-02-13 Thomas Jefferson University TCL-1 protein and related methods
US5985598A (en) * 1994-10-27 1999-11-16 Thomas Jefferson University TCL-1 gene and protein and related methods and compositions
DE19610255B4 (de) * 1996-03-15 2004-11-04 Universität Heidelberg Verfahren zur Herstellung von Nukleinsäuresequenzen und Verfahren zum Nachweis von Translokationen zwischen Chromosomen
US5824478A (en) * 1996-04-30 1998-10-20 Vysis, Inc. Diagnostic methods and probes
US6994971B1 (en) * 1999-10-08 2006-02-07 University Of Utah Research Foundation Particle analysis assay for biomolecular quantification
JP5059271B2 (ja) 2000-03-31 2012-10-24 パーデュー・リサーチ・ファウンデイション リガンド免疫原複合体を用いる処置方法
WO2003018835A2 (en) * 2001-08-23 2003-03-06 Hvidovre Hospital Method for rapid detection of haplotypes
JP4708029B2 (ja) 2002-10-11 2011-06-22 エラスマス・ユニバーシティー・ロッテルダム Ｐｃｒベースのクローン性研究のための核酸増幅プライマー
WO2005059176A1 (en) * 2003-12-15 2005-06-30 Institut Pasteur Repertoire determination of a lymphocyte b population
ATE421998T1 (de) * 2003-12-15 2009-02-15 Pasteur Institut Ermittlung des repertoires von b-lymphozyten populationen
CZ302581B6 (cs) * 2010-06-04 2011-07-20 Masarykova Univerzita Zpusob detekce chromozomální translokace t(11;14)(q13;q32) a oligonukleotidy pro použití pri tomto zpusobu
JP7279633B2 (ja) * 2017-03-29 2023-05-23 東洋紡株式会社 核酸の検出方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4701409A (en) * 1984-11-15 1987-10-20 The Wistar Institute Detection of B-cell neoplasms
CA1272443A (en) * 1985-02-22 1990-08-07 Nanibhushan Dattagupta Solution-phase dual hybridization assay for detecting polynucleotide sequences
US5024934A (en) * 1988-03-14 1991-06-18 The Board Of Regents, The University Of Texas System Detection of minimal numbers of neoplastic cells carrying DNA translocations by DNA sequence amplification
DE68919706T2 (de) * 1988-05-10 1995-04-13 Du Pont Verfahren zum schnellen nachweis von nukleinsäuren.
DE3815913A1 (de) * 1988-05-10 1989-11-23 Sandoz Ag Neue reninhemmer, verfahren zu deren herstellung und deren verwendung

Also Published As

Publication number Publication date
WO1992019775A1 (en) 1992-11-12
ITRM910286A0 (it) 1991-04-29
EP0583284A1 (en) 1994-02-23
AU1649992A (en) 1992-12-21
CA2109518A1 (en) 1992-10-30
JPH06509464A (ja) 1994-10-27
ITRM910286A1 (it) 1992-10-29
AU666648B2 (en) 1996-02-22

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Legal Events

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0001 Granted
TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19960423